Before initial use of this instrument, please carefully
Contents
1. 18 3 Main Interface x p 22 3512 o a e ERR e CUR Te det B e de ces REP UG ERE RU 22 3 2 Basic OperatiOn ce aeree pas ee bote b IR esce eto etie e Me estes 22 SASAR DEIT AATA U EE E E EE E 25 e i Aaaa o E E 26 959 Koo MNAL IATE OR T EEEE 29 Chapter VI Firmware upgrading 32 1 Device config ration u n pe pente ete pede poche usu Eo eee ze da 32 2 Way to upgrade instrument software 32 Chapter VII Failure analysis and handling U U 39 1 Failure analysis and handling 39 Attachment 1 Wiring 41 Attachment 2 Value and Meaning of Parameter Setting 42 LifePro Thermal Cycler Introduction Chapter Introduction The contents in this chapter include introduction of PCR technology examples of PCR application introduction of Thermal Cycler and features of LifePro Thermal Cycler 1 Introduction of PCR technology Polymerase chain reaction PCR is a nucleic acid amplification that simulates natural DNA replication in vitro It takes 2 DNA chains to be amplified as template uses a pair of artificially synthesized oligonucleotide primer as mediator and amplifies certain DNA fragment quickly and specifically by thermostable DNA polymerase Ever since PCR2. Temperature control precision lt 0 1 Temperature fluctuation lt 0 2 Maximum gradient 30 C Minimum gradient 1 Scope hot lid temperature setting 30 C 110 C Scope of hot lid working temperature 105 C 5 C File saving amount 299 pieces Energy consumption maximum input power 600W Operation mode Continuous operation mode Outline dimension 351mmx279mmx250mm LxWxH Fuse 250V 8A 5x20 PC interface RJ45 Weight kg 10 LifePro Thermal Cycler Features Caution If the hot lid temperature setting is lower than module temperature setting the hot lid temperature shown on screen is higher than hot lid setting Software functions Edit the file including file number temperature and time of each segment cycle times setting file view revision and deletion automatic file connection gradient temperature setting the maximum paragraph step and cycle times for editable file are 5 16 and 99 respectively The scope for gradient temperature setting is 1 C 30 C Save the file Revise the file adjust the rate for temperature rise fall refine temperature and time for cycling automatically and display and calibrate the time Display the temperature time cycle times setting and real time value at file running including sound prompt total rest time estimation for file running failure protection and alarming Stop the file running including pause the
3. Time indicate the default system time after system start 1 2 2 Default File Default User indicates the default running program name and user after system start 1 2 3 Control Mode indicates the default temperature control mode after system start including Block and Tube choices a Block mode only concerns the temperature control for module during temperature rise fall Now that the temperature rise fall of reagent lags behind the module the actual temperature change of reagent is different from the program we set The actual time that the reagent reaches the set temperature is far less than the set time see figure below for relation between module temperature and reagent temperature 100 97 94 91 88 85 82 79 76 73 Temperature module reagent LifePro Thermal Cycler Instruction for operation b Tube mode considers the temperature rise fall of reagent that lags behind the module There is an overshoot when it reaches the set temperature so that the reagent reaches the set temperature faster At the set time same with Block mode obviously the actual time that reagent reaches set temperature is prolonged See figure below for relation between module temperature and reagent temperature 100 97 94 91 88 85 82 79 76 73 70 module reagent Temperature
4. Mode Serial Number 3898 Firmware Ver Ver 4 0 BIOS Ver Ver 2 1 Status Data Mode Settings Click the Modify check box to modify configuration x Cancel Select Real com Mode under Operating Mode choices Operating Node 1 Port s Selected 1st port is Port 1 Operating Mode TCP Server Mode Real COM Mode TCP Server Mode Server TCP Client Mode UDP Mode TCP Server Mode Settings Miscellaneous Optional Local TCP Port 4001 TCP Alive Check Timeou 7 REO Select Real Com Mode Inactivity Timeout Max Connection 0 0 65535 ms Data Packing Optional Delimiter 1 0 ff Delimiter 2 O ff Force Tx Timeout 0 65535 ms J OK X Cancel Click COM MAPPING in function list LifePro Thermal Cycler Firmware upgrading Hetwork Enabler Administrator CON Mapping File Function COM Mapping View Help Mapping 0 COM add ta rg et device NET IP Address Parameter Mapping Port Monitor IP Address Report remove target device 2008 1 3 14 26 33 Found Network Enabler Module s 1 2008 1 3 14 35 18 Configuration Changed NE 4110S 00 30 E8 10 E2 B7 2008 1 3 14 35 39 Configuration Changed NE 4110S 00 30 E8 10 E2 B7 2008 1 3 14 35 49 Configuration Changed NE 4110S 00 30 E8 10 E2 B7 2008 1 3 14 37 22 Configuration Changed 41105 00 90 E8 10 E2 B 7 Enabler Administrator CON Mapping
5. Caution Program version might upgrade without notice User can visit www bioer com cn to check and download the latest program version LifePro Thermal Cycler Failure analysis and handling Chapter VII Failure analysis and handling This chapter introduces the failures the instrument might have and the way to analyze and handle 1 Failure analysis and handling No Failure Analysis Handling Check power and Power is not connected connect The screen doesn t turn on and a Fuse is burnt iu i 20N 1 du du sound is not heard after power on Switch is damaged Replace switch Others Contact supplier manufacturer A du du sound is heard after power The plug that connects 2 on Please insert Block is shown on module and host Screen a du alarm is heard machine is loose Please check error is shown on screen while file running and one of the following is prompted Module sensor is 3 Temperature sensor disconnect damaged in n Temperature sensor disconnect contact Temperature sensor2 disconnect Temperature sensor3 disconnect Please check error is shown Heater sensor is h Contact supplier or 4 screen while file running and Heat damaged or in poor P I manufacturer sink sensor disconnect is prompted contact Please check error is
6. MAC Address IPAddress _ Configuratiaq tig COM Mapping Monitor Port Monitor 3X9 IP Address Report configuration Message Log 0 Monitor Log 0 No Time Description 5 2008 1 3 14 15 25 Double click network number searched in list column open property page and select Network Configuration Information Auto Warning IPAddress Repot Password DigitallO SerialCMD Model 1 f Operating Mode NE 41105 Basic Sera perating Accessible IPs MAC Address Modify 00 30 E8 10 E2 B7 um Mode masu Serial Number 3898 Firmware Ver Ver 4 0 BIOS Ver Ver 2 1 Status Data Mode Settings Click the Modify check box to modify configuration OK x Cancel Select Server Mode under Operating Mode LifePro Thermal Cycler Firmware upgrading Operating Node 1 Part s Selected 1st port is Port 1 Operating Mode TCP Server Mode Real COM Mode Server TCP Server Mode TCP Client Mode UDP Mode TCP Server Mode Settings Local TCP Port 4001 Max Connection 0 65535 ms Data Packing Optional Delimiter 1 0 ff Hex Delimiter 2 0 ff Force Tx Timeout 0 65535 ms y OK Cancel Click OK close Network Enabler Administrator 12 Restart the instrument access interface for instrument system parameter setting and set system parameters again
7. C 1 2 4 Hotlid indicates the on off status of hot lid 1 2 5 Key Sound and End Sound indicate the sound to press key and the sound at program finish respectively See attachment 2 Value and Meaning of Parameter Setting of this manual for specific index of each parameter above Caution 1 Press Save to confirm the system parameter setting or the system parameter won t change 2 Itcalibrates the clock inside the instrument as per Beijing time upon ex factory 3 Itis recommended to check the date and time again for the first time to use 1 3 Gradient module temperature distribution features The gradient module uses 3 line temperature control unit The gradient temperature is distributed as curve as shown in figure below the set temperature is 40 and the set gradient temperature is 20 The temperature gradient in each hole row on module is nor uniform The temperature difference on hole rows at center is higher than the that at outside Therefore at gradient module operation the temperature of each hole row shown on instrument governs Gradient graphs 60 58 56 52 50 48 46 44 42 40 Temperature C N C 5 6 7 8 9 10 11 12 Column left to right LifePro Thermal Cycler Instruction for operation 2 How to input output PCR program 2 1 Connect to USB flash memory USB connection interface Press UsbLink in main interface to access the interface for USB connection If U
8. 00 390 E8 10E2 87 input IP address Serial Number 3898 Netmas 255 255 255 0 input subnet mark Gateway Firmware Ver et Ver 4 0 P Configuration Static DNS Server 1 BIOS Ver Ver 21 DNS Server 2 Status Modify Data Mode public Click the Modify check box to modify configuration OK Open the property page select the Operating Mode items click the settings button User s Manual LifePro Thermal Cycler Connection to computer Configuration Information Auto Warming IP Address Report Password Digital lO Serial CHD Model perating Mode 41105 Basic Network perating Accessible IPs MAC Address Modify 00 30 E8 10 E2 B7 le Mode Serial Number 3898 Firmware Ver Ver 4 0 BIOS Ver Ver 2 1 Status Data Mode Settings Click the Modify check box to modify configuration OK x Cancel Select TCP Server Mode under Operating Mode Operating Node 1 Port s Selected 1st port is Port 1 perating Mode TCP Server Mode Real COM Mode Server Mode TCP Client Mode UDP Mode TCP Server Mode Settings Miscellaneous Optional Local TCP Port 4001 TCP Alive Check Timeout 7 ES Select TCP Server Mode 33 min Inactivity Timeout Max Connection 0 0 65535 ms Data Packing Optional Delimiter 1 O ff Delimiter 2 O ff Hex Force Timeo
9. Cancel Step 5 click Install Step 2 click Next m Setup Network Enabler Administrator Select Destination Location Where should Network Enabler Administrator be installed Setup will install Network Enabler Administrator into the following folder To continue click Next If you would like to select a different folder click Browse Browse J Atleast 2 5 MB of free disk space is required lt Back Next gt Cancel Step 4 Click Next Setup Network Enabler Administrator Ready to Install Setup is now ready to begin installing Network Enabler Administrator on your amp computer cS Click Install to continue with the installation or click Back if you want to review or change any settings Destination location C Program Files Network Enabler Administrator Additional tasks Additional icons Create a desktop icon Cancel Install Step 6 complete installation click Finish User s Manual of LifePro Thermal Cycler Connection to computer A m Setup Network Enabler Administrator Setup Network Enabler Administrator Installing Please wait while Setup installs Network Enabler Administrator on your computer Completing the Network Enabler Administrator Setup Wizard Setup has finished installing Network Enabler Administrator on your computer The application may be launched by selecting the installed icons Extracting files C Progr
10. file running stop the file running recover automatically after power off Caution The above mentioned software function is merely for reference The software function would be modified without notice LifePro Thermal Cycler Preparations Chapter IIl Preparations This chapter introduces the structure of instrument keyboard for operation function of buttons and preparations before start Please get familiar with the contents in this chapter for the first time to use this instrument 1 Structure diagram Note DHot lid Keyboard screen USB interface Ventilation hole O Block CORJA5 interface 8 Power switch 9 Power socket with fuse Caution The fuse specification must be 250 8A 5x20 Use qualified product to replace the fuse Contact fuse supplier or manufacturer when necessary LifePro Thermal er Preparations 2 Keyboard diagram 3 Instruction for buttons N Pi ABC DE GHI JK e Character Key to input um characters press button continuously to input letters Display mode switch key switch display mode between curve and text during running Plus minus key switch the plus minus for program temperature editing and time refining Cursor left right key press once to move the cursor left right Cursor up down key press once to move the cursor up down Confirmation key press button to accept the setting value on screen at file s
11. for paragraph editing where you can set cycle amount and cycle starting segment The figure shows that it cycles from segment 1 to segment 3 and cycles for 10 times lt Repeat to press Cycle key to add paragraphs maximum is 5 Press Delete to delete the current paragraph immediately Move the cursor up down to enter previous or next paragraph lt gt lt gt 0 0 0 00 02 00 0 0 0 00 01 45 0 0 0 00 00 00 0 0 0 00 Press Save key to access the interface for file saving Press Back key to return to the interface for file list Special caution 1 Please save the new edited PCR program before running or the file can t run 2 If Time is set as hot lid is closed automatically when module temperature control works Cycle1 From to e e e Cycle2 From to Cycle5 From to Relation of segment and paragraph in the file LifePro Thermal Cycler Instruction for operation Interface for file saving Press character key in this interface to input file name press confirmation key to move the cursor forward Select User and File Name by up down key Delete character by left key move cursor by right key Press Caps Key to shift between capital and lower Hotlid off 2009 05 1 10 05 00 lee case At upper left on screen it indicates User PCR1 capital or lower case as shown in the figure m File Name PCR1 lt Press Main key to return to main
12. is correct it shows the dialog box below File Saved If not it shows File input error sc 3 Output file to USB flash memory Press Back to return to interface of USB flash memory connection lt Press cursor up down to select copy file user lt Turn page by cursor left right key if user number is more than 30 lt Press View to see device user file list lt Press Back to return to interface of device user list Press cursor up down key to select copy file lt Turn page by cursor left right key if user number is more than 30 lt Press to output file lt If copy is correct it shows the dialog box below File Saved If not it shows File output error Caution Instruction for operation USB file list Hotlid off 2009 05 1 10 05 00 Filet File2 Press Left Right Key to Previous Next Page Copy Hotlid off 2009 05 1 10 05 00 Local User1 User2 Press Left Right Key to Previous Next Page Device file list Hotlid off 2009 05 1 10 05 00 Local Filet File2 Press Left Right Key to Previous Next Page Copy USB reads writes 500 users each user has 2 000 files USB reads writes bioer file at USB root directory in fixed directory 1 2 3 Each user is the next subfolder under bioer fi
13. of GB4793 1 Standard and it is for indoor use a Instrument earth In order to avoid electrical shock accident the input power wire of instrument shall be reliably earthed This instrument uses 3 core earth plug and this plug is provided with a third earth leg is merely used for earth type power socket and is a safety unit If the plug is impossible to insert into the socket the socket shall be correctly fixed by electrician otherwise the earth plug would lose safe protection function b Keeping apart from the live circuit The operator shall not disassemble instrument without permission Only qualified professionals are allowed to replace elements or adjust the machine inside Replacement of elements under energizing state shall be prohibited c Use of power supply Before turning on AC power make the power voltage consistent with the instrument required one a 10 deviation is allowed Make the rated load for power socket not below instrument s maximum load d Power wire Generally this instrument uses accompanied power wire The damaged power wire shall be replaced by one of the same type and the same specification It can t be repaired The power wire shall neither be compressed nor be laid in gathered personnel location e Insertion and withdrawal of power wire At insertion and withdrawal of power wire the plug shall be correctly held with hand At insertion the plug shall be completely and tightly inserted into the soc
14. off the power supply withdraw the power plug from the power socket contact the supplier or ask qualified repair personnel for treatment Liquid drops into inside of the instrument The instrument is rained or watered The instrument works abnormally especially generates abnormal sound or odor The instrument is dropped or its casing is damaged The function of instrument obviously changes 3 Instrument label BS HOT SURFACE Warning The instrument is adhered with mark SURFACE During operation of instrument or a period immediately after completion of running of a program any portion of body shall not be contacted with metallic portion module or hot lid nearby the said mark otherwise skin would be burnt 4 Maintenance of instrument In order to ensure full contact and good heat conduction between the test tube and the conical holes the conical hole on the module shall be regularly cleaned with clean soft cloth soaked with a little absolute alcohol The stained surface of this instrument if any shall be cleaned with soft cloth soaked with cleaning paste Warning At cleaning of instrument its power supply shall be cut off The instrument surface shall not be cleaned with corrosive cleaning agent 5 After sales service The warranty content and scope are shown in the warranty sheet Note After unpacking immediately check the goods against the packing list In case of damaged or miss
15. 00 01 00 Seg 6 550C 50 00 01 00 setting details Select the file to be copied Select Copy File menu under File menu User s Manual LifePro Thermal Cycler Connection to computer Paste file Select User item under device list File can t be overlaid if it is already existed and open Prompt File is only copied and pasted between computer and device File can t be overlaid if it is already existed and open Delete file Select file to be deleted Select Delete File menu under File menu Prompt File can t be deleted if it is open Change file name Select file to change name Select Change File Name menu under File menu Input file name and click OK Prompt File name can t be changed if it is open Change File Name Do you want to change file name of Computer gt TTTT gt 1 Enter new file name The file name can contain only letters and numbers Z OK x Cancel Change file password Select file to change password Select Change File Password menu under File menu to bounce dialog box Input old password and new password click OK Prompt File name can t be changed if it is open User s Manual LifePro Thermal Cycler Connection to computer Change File Password Do you want to change password of Computer gt TTTT gt 1 Enter old password Enter new password The password can contain only numbers Enter new passw
16. 37 22 Configuration Changed 41105 00 91 Double click the network number setting property mapping page it searched in list column and select Advanced Settings setting as shown below User s Manual LifePro Thermal Cycler Connection to computer CON Port Settings Port Number 1 Part s Selected 1st port is Port 1 Basic Settings Advanced Settings Serial Parameters Tx Mode Classical FIFO Fast Flush Only Flush Local Buffer Select Serial Parameters setting as shown below CON Port Settings Port Number 1 Selected 1st port is Port 1 Basic Settings Advanced Settings Serial Parameters Baud Rate 9500 B Parity Data Bits Stop Bits Flow Control X Cancel Click OK click confirmation apply the setting finally click remove device and complete mapping setting Caution 1 Several devices online can t use a same IP address 2 Configure the device again if this instrument is used for the first time 3 Set same subnet for several devices online 4 The device doesn t support the function of acquiring IP address automatically 5 Use switch to connect for several devices online use cross network cable to connect if HUB is used User s Manual of LifePro Thermal Cycler Connection to computer 3 Main interface 7 f GenePro Filter all Y eq106 Equipment state display z eq104 96G s a Main display area 54 System Gy History
17. 84 B 51 5 a test yer B Run File Set system parameters Double click Settings sub item under System items to set system parameters GenePro User File Tools Help 22 5 5 3300506 31328156 2 Q Computer eq104 gt system setting El Fiter SG ut Default B ss 106 B okfile Default hotlid temperature oper 51 C s B fl Beep alarm when run finish du eq104 96G eq106 Key 8 b User Temperature control mode 54 System eq104 User Default file a huang Default user a 984 B 51 5 test System M History B RunFile Module A Default hotlid temperature Beep alarm when run finish Key beep Temperature control mode Default user Default file Module B Default hotlid temperature Beep alarm when run finish Key beep Temperature control mode Default user Default file User s Manual LifePro Thermal Cycler Connection to computer 3 3 User menu How to 2 Create new file 22 Change user name 2 Paste user 2 Delete user 22 Import user 2 Export user Create a user Select device name sub item in device list or any user name sub item in device name sub item Select create user menu under User menu it bounces a user dialog box Input user name and click OK Create User Do you want
18. Caution The Bioer Co reserves the right to modify this manual at any time without notice The manual contains copyright protected and patented material Without prior written consent from Hangzhou Bioer Technology Co Ltd any part of the manual shall not be duplicated reproduced or translated into any other language Before initial use of this instrument please carefully read this manual File No BYQ60630000000ESM File version July 10 2009 Version 1 0 LifePro Thermal Cycler Important Notes Important Notes 1 Usual practice Caution Important information is contained in any item and should be carefully read Failure to observe the instruction would result in damage or abnormal function of instrument Warning The warning message requires extremely careful operation of a certain step Failure to observe the instruction would result in serious personal injury 2 Safety During operation maintenance and repair of this instrument the following basic safety notes shall be observed In case of failure to follow these measures or the warnings or notes indicated herein the basic protection provided by the instrument its safety criteria of design and manufacture and its predicted use range would be impaired Hangzhou Bioer Technology Co Ltd shall be irresponsible for the consequence resulted from user s failure to observe the following requirements Note This instrument conforms to Class I and Protection Class IP20
19. File Function COM Mapping View Help DONS s Function COM Mapping 0 COM Network Enabler Admit IP Address Parameter Configuration Mapping Monitor Port Monitor IP Address Report 2008 1 3 14 26 33 Found Network Enabler Module s 1 2008 1 3 14 35 18 Configuration Changed 41105 01 2008 1 3 14 35 39 Configuration Changed 41105 01 2008 1 3 14 35 49 Configuration Changed NE 41105 0 2008 1 3 14 37 22 Configuration Changed 41105 00 90 E8 10 E2 B7 Click button for add target device N Network Enabler Administrator CON Mapping File Function COM Mapping View DON Function COM Mapping 1 COM Network Enabler Admi No _ Model IPAddress _ Pon COM Port Mode Parameter Configuration 1921680245 17 LHi Performance FIFO Ena 3600 None 8 1 Nor if COM Mapping Monitor Port Monitor AX IP Address Report Message Log 5 Monitor Log 0 Time Description 2008 1 3 14 26 33 Found Network Enabler Modulefs 1 2008 1 3 14 35 18 Configuration Changed NE 41105 00 90 E8 10 E2 87 2008 1 3 14 35 39 Configuration Changed NE 4110S 00 90 E8 10 E 2 87 2008 1 3 14 35 49 Configuration Changed NE 4110S 00 90 E8 10 E 2 87 2008 1 3 14 37 22 Configuration Changed NE 41105 00 90 E8 10 E2 87 LifePro Thermal Cycler Firmware upgrading Double lick network number setting mapping property pa
20. GP Setting Equipment list 3 1 Tool bar Amend user name Input file Copy file Change file code Grads calculate Output user Paste user New file Delet file Link equipment T yd eu Input user Copy user Copy user Save file Equipment setup Paste file Setup user Delet user Edit file file Disconnect link 3 2 Basic operation Open device or this computer s file Click run Select type and volume for test tube Click OK to run Prompt Select device before running if file is saved in computer 22 User s Manual LifePro Thermal Cycler Connection to computer View running file Run File A B is shown below the device name when device is running file Double click Run File A B to see running file contents and device running status Prompt Click pause stop to pause stop equipment GenePro User File Tools Help 28828668 23008 30 23415 Sra a Computer eq104 gt Run File E Fiter fan gt Et 7 Temp tine AutsbetionTerp AutoExtienrime sce eqi 6 2 20 0 00 20 4 0 B 00 50 4 0 2 3 0 00 20 4 0 2 eq104 96G 2 eq106 4 0 00 40 40 3 Y _ User 54 System eq104 5 User huang 984 B 51 3 5 Begin Segnent End Segnent Cycle Times a8 test 4 System B B okfile Cuvette volume 60ul Cuvette type 0 5ml State Run
21. SB Hotlid off 2009 05 1 10 05 00 flash memory is inserted as the figure at left shows Press Back to return to main The Instrument has Connected UsbDisk interface Please Choose Input or Output File lt V Press Input to select file input from USB flash memory it shows USB user list Press Output to select to output file Press F1 to Back USB flash memory it shows local file Press F4 to Input File user list Press F5 to Output File Back Interface of USB connection fail If no USB flash memory is inserted as the Hotlid off 2009 05 1 10 05 00 figure at left shows press Back to return to main interface No disk connection Press F1 to Back T 2 Input file from USB flash memory Press Back to return to interface of USB flash memory connection Press cursor up down to select copy file user Turn page by cursor left right if user number is more than 30 Press View to see USB file list Hotlid off 2009 05 1 10 05 00 User1 User2 Press Left Right Key to Previous Next Page LifePro Thermal Cycler Press Back to return to interface of USB flash memory user list Press cursor up down to select copy file Turn page by cursor left right if user number is more than 30 Press Copy to input file If copy
22. alias click Save to save setting Prompt The setting comes into valid only after software restart P setting must match the device User s Manual of LifePro Thermal Cycler Connection to computer Server Setting 9 192 168 0 222 001 192 188 0 243 amp Delete 192 168 0 240 003 192 168 0 241 004 td Save Cf Close LifePro Thermal Cycler Firmware upgrading Chapter VI Firmware upgrading 1 Device configuration Connect device to computer by network cable cross B type connection network cable 2 Way to upgrade instrument software 1 Power on open instrument press F1 on instrument to access software upgrading preparation status and find Prepare to upgrade on screen 2 Run Network Enabler Administrator program It communicates through virtual device mapping while instrument upgrading mE N Network Enabler Administrator Configuration File Function Configuration View Help 22 eon search button Function Configuration 0 Network Enabler Module s Network Enabler Admit No Model MAC Address IP Address Status Configuration M Mapping uy 4 Monitor Port Monitor 10 IP Address Report configuration mapping lt gt Message Log Monitor Log No Time Description How 2008 1 3 14 15 25 3 Configuration Click s
23. am Files Network Enabler Administrator NEADMIN exe ITITITITITITITITITITITITITITITITIT Click Finish to exit Setup 2 2 Device configuration 2 2 1 Connect the device to computer by network cable cross B type connection network cable compute device 2 2 2 Run Network Enabler Administrator Monitor Port Monitor IP Address Report mapping No Tm Description Now 2008 1 3 14 15 25 User s Manual LifePro Thermal Cycler Connection to computer 2 2 3 Configuration Click the search button and the tool searches instrument automatically Network Enabler Administrator CON Mapping Eile Function COM Mapping View Help 9gom en Function COM Mapping 1 COM J Network Enabler Admit Model IP Address Port Port Mode Parameter Configuration 3500 None 8 1 Nor fif COM Mapping Monitor Port Monitor X IP Address Report instrument network number it searched lt gt lt Message Log Monitor Log No Time Description Double click the network number in the list column that it searched open the property page and select network items Configuration Information Aute Warning IPAddress Report Password DigtallO Seria CMD Model i Network 41105 Base Seis Operating Mode Accessible IPs Modify MAC Address IP Address 182 168 0245
24. earch button the tool searches device automatically LifePro Thermal Cycler Firmware upgrading Network Enabler Administrator CON Mapping File Function COM Mapping View Help oomen Function COM Mapping 1 COM Network Enabler dmi No IP Address Parameter Configuration 9600 None 8 1 No Monitor Port Monitor X IP Address Report Network device number it searched Message Log Monitor Log 0 No Time Description Double click the network number searched on list column open the property page and select network Configuration Information Auto Warning IPAddress Repot Password DigitallO SerialCMD Model I perating Mode NE 41105 Basic perating Accessible IPs MAC Address Modify 00 30 E8 10 E2 B7 Pot as Mode Heal Mode Serial Number 3898 Firmware Ver Ver 4 0 BIOS Ver Ver 2 1 Status Data Mode Settings Click the Modify check box to modify configuration OK X Cancel Open the property page select Operating Mode and click setting LifePro Thermal Cycler Firmware upgrading Configuration Information Auto Waming IPAddress Report Password DigtallO SerialCMD Model z perating Mode 41105 Basic Network Serial erating Accessible IPs MAC Address Modify 00 90 8 10 E2 B7 Pot Alias OP Mode
25. ed goods please immediately contact the supplier After qualification of acceptance properly fill the product acceptance sheet and send or fax the copied sheet to the supplier for filing and maintenance After unpacking the packing box and packing materials shall be well kept for future repair For the damage of instrument occurring during transporting to the repair department due to improper package Hangzhou Bioer Technology Co Ltd shall bear no liability User s Manual of LifePro Thermal Cycler Content CONTENT Chapter Introduction 1 1 Introduction of PCR 1 2 Example of PCR technology application 1 3 Features of LifePro Thermal Cycler 1 4 Use of LifePro Thermal 1 leurucdirul c 3 1 Normal working conditions 3 2 Transportation and storage conditions 3 3 uu Q PM 3 Software f fictlons tete eene d Ee iret ounce cfe eo dex duda 4 Chapter 5 1 Structure diagram ge eet eec b eae eee cr tee ee Coe 5 2 Keyboard 6 3 Instruct
26. er repetitive sequence genetic map construction detect DNA polymorphism sperm drawing physical map construction sequencing expression map and etc Forensic medicine crime scene specimen analysis HLA DQ analysis and etc Oncology pancreas cancer rectal cancer lung cancer thyroid cancer melanin cancer hematological malignant tumor and etc Histology population biology genetic cluster research evolution research animal protection research ecology environmental science experimental genetics and etc Paleontology archeology and museum specimen analysis and etc Botany zoology animal inflectional disease diagnosis plant pathogen detection and etc 3 Features of LifePro Thermal Cycler LifePro Thermal Cycler is a PCR instrument with multiple functions and several applications Below are its features Use advanced thermoelectric refrigeration technology from ITI of US and thermoelectric refrigeration elements made under quality management of Japan which are steady and reliable in quality Temperature can be changed as fast as 4 C second The speed to increase or decrease temperature can be set as per need of experiment Several modes for temperature control better compatibility of reagent Large LCD screen user friendly operation interface simple and easy operation Power off protection available programs can continue after power recovery Height and pressure of hot lid can be set for different reaction
27. etting Menu key press button to accept the item above the button on screen LifePro Thermal Cycler Preparations 4 Check before start Check the following before insert the plug and power on the instrument 1 Whether the voltage of power supply matches the voltage required by instrument see Chapter II for requirements of power supply 2 Make sure the power line pug has inserted in power socket reliably 3 Earth the power line reliably 5 Start Power on turn power to the amplifier gives a du du sound and the fan starts to run which indicates the power supply is available Self testingeeeeee is shown on screen and then the instrument takes about 1 to 2 minutes for self testing Please wait with patience The main interface appears if no problem is found then you can edit view modify or delete the PCR amplification file LifePro Thermal Cycler Instruction for operation Chapter IV Instruction for operation This chapter explains how to edit view modify and delete the PCR amplification file how to run PCR amplification file and how to set the instrument system parameters in detail Warning If the instrument sound or display is abnormal after start or failure alarm and indication is shown during self testing please turn off the power immediately and contact the supplier Caution If the sample amount for test is less than countersink amount on module please distribute the sample test tube as un
28. ge searched in list column and select Advanced Settings setting as shown below CON Port Settings Port Number 1 Part s Selected 1st port is Port 1 Basic Settings Advanced Settings Serial Parameters Mode Classical Fast Flush Only Flush Local Buffer Jf OK X Cancel Select Serial Parameters setting as shown below CON Port Settings Port Number 1 Part s Selected 1st port is Port 1 Basic Settings Advanced Settings Serial Parameters Baud Rate Parity Data Bits Stop Bits Flow Control y OK Cancel Click OK click confirmation and apply the setting 4 Run Upgrade exe 5 Press select upgrading program bin in computer 6 Press start the computer begins to upgrade software in instrument and the instrument screen prompts Upgrade begin 7 The instrument prompts Configuring Mode during software upgrading 8 The instrument prompts Upgrade finished when software upgrading is finished LifePro Thermal Cycler Firmware upgrading 9 Firmware upgrading is finished 10 Click remove device remove device mapping 11 Recover the configuration Open Network Enabler Administrator select configuration function Network Enabler Administrator Configuration Bee File Function Configuration View Help 222 een Function Configuration 0 Network Enabler Module s j Network Enabler Admit Model
29. iform as possible In this way it ensures that the hot lid presses at the test tube top steadily at file running the module bears uniform load and the temperature change in test tubes keeps uniform Caution Turn the knob at anti clockwise direction as per diagram before covering the lid clockwise rotation for pressing anti clockwise rotation for loosing until a dang dang sound is heard Cover the lid and turn the knob at clockwise direction until a dang dang sound is heard and then turn another step When a dang dang sound is heard when knob is rotated at first regardless of clockwise or anti clockwise press the unlocking device by finger turn the knob at the opposite direction for two steps and then rotation knob on unlocking device can be released 1 How to run PCR program Power on the thermal cycler gives a du du sound which indicates the power supply is available Self testing is shown on screen and the instrument takes about 1 to 2 minutes for self testing Please wait with patience The main interface appears if no problem is found Main interface Shown on the upper left of main interface is the status of hot lid setting Hotlid off 2009 05 1 10 05 00 Shown in the middle of main interface is the information such as the Bioer information software version module type default BIOER program default user control mode sample amount and so on yee TM RUE 5 File to access the i
30. ion for buttons 22 ta t tert ds Nadi e Penn t Etpe recen FRENIS E AEn daii 6 4 Check before start opto ete Ib te eter ite Pepe edu 7 EVI c 7 Chapter IV Instruction for operation U UU eene nnne nennen nnn uuu uuu 8 1 How to run PCR program s eee ict i sui eee e dti ee eed e da lal tne Pe ed a Sede 8 1 1 How to edit POR programs Certe tree o ee revera eet ve ee od erc Dave ea S tea 9 1 2 How to set system 12 1 3 Gradient module temperature distribution features 13 2 How to input output PCR 14 2 1 Connect to USB flash 14 2 2 Input file from USB flash memory 14 2 3 Output file to USB flash 15 Chapter V Connection to computer 16 1 Software Installation Reit ete e E femi dete denas 16 2 Online setlflRgu uuu 16 2 1 Toolsoftware installation 2 reto upas nn ett e aep D ri bee PO bre Dani dn 16 2 2 Device configuration 17 2 2 1 Connect the device to computer by network cable cross B type connection 17 2 2 2 Run Network Enabler Administrator 17 22 3 Configuration te nie ER Rara
31. ket and at withdrawal heavily pulling the wire shall not be allowed f Placement of instrument This instrument shall be fixed in a low RH and low dust place apart from water source e g water pool and water pipeline and the room shall be well ventilated and free of corrosive gas or and interference of strong magnetic field The instrument shall not be placed in a wet or dusty location The openings on this instrument are for ventilation circulation and in order to avoid over heat of the LifePro Thermal Cycler Important Notes instrument body they shall not be blocked or covered When a single set or several sets of instrument are used the interval between its surrounding ventilation opening and its nearest object shall not be below 50cm or 100cm Also don t use the instrument on loose or soft surface or the air inlet at instrument bottom might be blocked Excessive temperature would impair the test performance or result in fault of instrument This instrument shall not be used in location subjected to direct sunshine The instrument shall be kept away from hot gas stove and all other heat source If the instrument is to be set aside for a long time the power plug shall be withdrawn and the instrument covered with soft cloth or plastic film to avoid entrance of dust 9 Notes during operation During test cares shall be taken to prevent liquid from dropping onto the instrument Caution In any of the following cases immediately cut
32. le 4 Don t plug USB flash memory in out quickly To protect device and USB flash memory from damage the device will terminate the USB function until next run if USB flash memory is plugged in out quickly 5 The operation for dual module is same It only connects USB flash memory at start interface of two modules User s Manual LifePro Thermal Cycler Connection to computer Chapter V Connection to computer 1 Software installation Click Setup exe in accompanied disc install the LifePro computer connection program which is able to control 30 LifePro instruments on one computer 2 Online setting LifePro is equipped with embedded online module inside and tools for setting are available 2 1 Tool software installation Step 1 click and run nead setup exe aag Welcome to the Network Enabler Administrator Setup Wizard Setup Network Enabler Administrator This will install Network Enabler Administrator 2 11 on your computer It is recommended that you close all other applications before continuing Click to continue or Cancel to exit Setup Cancel Step 3 select destination location click Next Setup Network Enabler Administrator Select Additional Tasks Which additional tasks should be performed Select the additional tasks you would like Setup to perform while installing Network Enabler Administrator then click Next Additional icons lt Back Next gt
33. lt 2 interface Password lt Press Save key to save the file When Password Check file is saved normally the system shows File have been saved Please continue another operation Press confirmation key to make it disappear If file has the Main Caps Save same name with another the system asks to name again Rename or continue to save Save Press number key to set and save password Press to access the interface for file running if Control Mode is Block now press to bounce the dialog box below if Control Mode is Tube now Select Sample Volume 050 It is the same with main interface confirm the password input the sample type and micro liter amount of sample size Caution 1 File name and user name are not case sensitive 2 Please remember the password 3 The password should be 0 9 numeric characters File running interface 1 As the file runs normally at Now Hot lid off 2009 05 1 10 05 00 Running keeps blinking The system User File Bioer Bioer shows File run over when running is completed lt Press Stop it shows Confirm stop running Temp 95 0C lt Select stop stop or continue Start as per information Time 01 00 lt Press Pause it shows Now pause running you can select continue Remain Time 00h56m Start as per information lt Press View File to view the edited PCR program lt Pre
34. name 11 characters in 11 Default User in system maximum length In this mode it comes to constant temperature status once it reaches Block target temperature the time that reagent temperature at target temperature is less than Temperature set 888 Fig 1 12 Control Mode control mode In this mode it comes to constant temperature status after 3 C overshoot once it Tube reaches target temperature the reagent temperature approaches target temperature quite fast see Fig 2 LifePro Thermal Cycler Attachment 2 Hot lid off Off 14 Holid Hot lid on 30 110 15 Key Sound Key tone yes no Yes No Buzz Prompt tone at 16 Run End Sound running finish Yes No yes no Continuous buzz Temperature Temperature Memorandum
35. ning Total time 04h 21m Remaining time 04h 21m Hotlid temp 20 5C Module temp 20 7C 54 55 0 53 400 40 secs eed ER 52 30 0 ect 50 secs Double click history sub item under System items to view historical information User File Tools Help 2238888 3030R08eR0DH TTaalos 12 2 Computer eq104 gt file run log a Fiterfal ga vi StartTine EndTine B 2007 11 30 15 57 22 Run satos okfile Bh 2007 11 29 10 15 53 2007 11 29 10 16 31 Complete EK u2 2007 11 29 10 14 17 2007 11 29 10 15 37 Complete eq104 96G 5 88 eq106 2007 11 29 10 13 39 2007 11 29 10 14 08 Complete mem User 2007 11 29 10 12 52 2007 11 29 10 13 27 Complete Lh System 5 9 eq104 User huang a 984 B 51 5 test System amp Setting B RunFile User s Manual LifePro Thermal Cycler Connection to computer View instrument device storage status Double click System to view instrument device storage status GenePro DER User File Tools Help s 9 lt e 3 3 3 i q 3 e 3 bal 5 8 amp 9 E Computer eq104 gt system state Har ET Sedul a Filter all B ss Total memory size 51200 eq106 okfile 5 B Used memory size 3072 z ad u2 F eq104 96G eq106 Free memory size 48128 p User Free memory scan store about 34 files 94 System eq104 User huang a 9
36. nterface for Block B 96G_ file list Default User BIOER lt Press System to access the interface Default File PCR1 for system parameter setting Control Mode Tube lt Press to confirm running mode and test tube type if file password is not asked press to bounce the interface below if file password is asked File UsbLink System Press number key to input the password lt Press Back to return to main interface lt Press Accept to access the interface for file running if Control Mode is Block now press Accept to bounce the dialog box for test tube volume setting if Control Mode is Tube now LifePro Thermal Cycler Press to select the test tube type in this interface lt gt lt gt Press Back to return to main interface Press Next to access the interface for file running if Control Mode is Block now press Next to bounce the interface below if Control Mode is in Tube mode now Press Character key to input the micro liter amount of sample size Different sample sizes have different overshoot temperature and lasting time when temperature control mode is Tube lt Press Back to return to main interface lt Press to access the interface for file running 1 1 How to edit PCR program Press File in main interface to access the interface for file list As shown in figure there are 3 files in the file list U
37. ord again Use empty password M OK X Cancel After file is edited click Save File item under File menu to save file Select user item on local computer Select Export File menu under File menu Select file to import and click Open Prompt File can t be imported if file is already existed and open Select file to be exported Select Export File menu under File menu Select path for export and click OK Prompt File can t be exported if it is open 3 5 Tool menu How to Connect device Disconnect Calculate gradient Change device IP and device name Connect device Select device not connected click Connect menu Prompt User s Manual LifePro Thermal Cycler Connection to computer One device can only connect to one computer Select device not connected click Disconnect menu Calculate gradient Select gradient menu Input gradient initial point and gradient value Select 8 12 96 holes as calculation module type Click Calc User File Tools Help 8232288 200 0 20 DM Computer Grad Temp a A 12 34 Temp 30 Gradient 24 Hotter 30 5 31 7 33 7 EER 54 0 Change device IP and device name Select Server Setting item under Tool menu to bounce the dialog box for setting Click Add to add line Click Delete to delete current line Fill in IP address and
38. r hot lid is damaged manufacturer Temperature sensor in hot lid is damaged Chip is in poor contact 10 Unusual character is shown screen gue Chip is damaged i Micro switch is Contact supplier or 11 Button on panel can t work damaged Tm anufacturep Set hot lid 12 Reagent in reaction tube evaporates Hot lid temperature is not set Hot lid is set as OFF temperature as per How to Set System Parameters in 1 2 of Chapter in this manual Reaction tube is not placed uniformly Adjust hole position on reaction tube try to place symmetrically Reaction tube is covered tightly Please cover reaction tube tightly before put into instrument 13 System parameter setting is invalid It didn t press Save to confirm system parameter setting Please refer to 1 2 of Chapter in this manual Caution 1 It is forbidden for user to open the case and check by oneself in warranty period Please contact supplier or manufacturer in time if any failure needs to open the case to check 2 Since temperature control characteristics rate of temperature rise fall stability fluctuation and etc of different brands and each instrument are specific and biotic experiment itself has uncertainty and vulnerable to outside influence PCR program that runs well on one instrument might fail to reach same effect on another Therefore please adjust PCR running prog
39. ram to get ideal performance once instrument is changed LifePro Thermal Cycler Attachment1 Attachment 1 Wiring diagram This diagram is for reference only and subjected to change without notice LifePro Thermal Cycler Attachment 2 Attachment 2 Value and Meaning of Parameter Setting Scope of setting 13 Sample Volume Sample size ul 10 200 No Parameter Meaning value Note Temperature 1 Temp C control point 4 0 99 0 Celsius Temperature MN Temperature control time 2 Time control tima press plus minus to is infinitely long close mm ss set before hot lid at same time 00 00 59 59 Rate 0 1 4 0 3 Ramp C S temperature Maximum rate of nu temperature rise fall Celsius second Temperature 4 Temp increment in each 0 0 9 9 cycle Celsius 5 TS Time increment in 0 00 9 59 each cycle m ss Cycle1 Paragraph number 1 5 x00 Cycle times in this 1 99 paragraph 6 First segment in X any segment Cycle1 x00 From 00 to From 00 this paragraph number already 00 existed to 00 Last segment in lt X 15 this paragraph Gradient 7 Grad temperature 0 30 Celsius 8 Date Date YY MM DD DU CUM Year month day 9 Time Time HH MM SS 00 00 00 23 59 59 Hour minute second 40 Default File Default file name 11 characters in in system maximum length Default user
40. rd is asked LifePro Thermal Cycler Instruction for operation lt Press to access the interface for file running when Control Mode is Block press to bounce the dialog box below if Control Mode is Tube now Select Sample Volume 050ul It is the same with main interface confirm the password input the sample type and micro liter amount of sample size Note 1 on upper right of user and file indicates this file is the default for system running 2 File can be modified in the interface for system parameter setting Interface for file editing Segment and paragraph can be edited in this The file i segments 2009 091 10 05 00 and paragraphs while segments contained in paragraphs Cycle times can be set for segments in paragraphs see figure below for relation Press up down or left right key to move the cursor the place where cursor is are highlighted Press Eee character key to change the parameter S m x HT setting Press plus minus key to change the LU plus minus symbol lt Press Seg key for segment editing where you can set temperature Temp lasting time Time rate for temperature rise fall Ramp temperature increment Cycle1 x10 From 01 to 03 in each cycle Temp and time increment in each cycle Time Press Delete to delete the segment where cursor is lt Press Cycle key
41. se the up down key to select user when blinking bar blinks in the User column and the file for selected user is shown in the File Name column shift to File Name by right click then the blinking bar blinks in the File Name column select corresponding User file by up down key lt Press Edit to edit the file pointed by cursor Press New File to edit a new file lt Press Delete it asks Confirm delete file select Delete or Back as per information lt Press to confirm running mode Hotlid off 2009 05 1 10 05 00 Instruction for operation Test tube type selection BIOER Bioer Technology Co Ltd Version 2008 1 1 Block Default User Default File PCR1 Control Mode Tube Select Tube Type 0 2ml Back Next Hotlid off 2009 05 1 10 05 00 BIOER Bioer Technology Co Ltd Version 2008 1 1 Block PERE Default User BIOER Default File PCR1 Control Mode he Select Sample Volume 050 Hotlid off Interface for file list 2009 05 1 10 05 00 No User OER BIOER2 BIOER3 File Name Save Time PCR1 2009 05 1 2009 05 1 2009 05 1 PCR3 New File Delete test tube type if file password is not asked press Run to bounce the dialog box below if file Input Password passwo
42. shown on Hot lid sensor is supplier or 5 screen while file running and Hotlid damaged in poor pp in manufacturer sensor disconnect is prompted contact Ventilation hole is Remove things that blocked block ventilation hole Dig terere Connection wire is ne pin 6 changes obviously or temperature loose fasten the bolt on control is not accurate Sur terminal if it is loose Refrigeration plate is Contact supplier or damaged manufacturer Q temperatur Please adjust ambient humidity is too high and temperature and Rate of module temperature fall slows Vie ape ration humidity properl condition of instrument y propery 7 down obviously it can t fall below Refrigeration plate is room temperature damaged Contact supplier Fan is damaged manufacturer doesn t run Temperature sensor is damaged Contact supplier 8 Moe tle can Or eoo All refrigeration plates manufacturer are damaged Hot lid status is set as OFF in system Set hot lid status as a tete Gail parameter setting certain temperature interface LifePro Thermal Cycler Failure analysis and handling Hot lid closes automatically since temperature control time is set as in file editing interface Set temperature control time in digital form Hot plug in is loose Heating element Contact supplie
43. ss Skip to skip the current temperature section and go to the next temperature LifePro Thermal Cycler lt Press to shift between running interface 1 and running interface 2 1 2 How to set system parameters Press System in main interface to access the interface for system parameter setting Press confirmation key to move the cursor to the next item Roll and select the items with by cursor up down key or press character key to input information for other items press cursor left right key to move the cursor Press Back to return to main menu lt Press Save to save parameters Special caution press Save to confirm after system parameter setting or the system parameter won t change dc Instruction for operation File running interface 2 Hotlid off 0 2 55 0 3c 72 0 4 04 0 1 00 02 00 01 45 00 00 File Name PCR1 User BIOER 1c Temp 00 0 Total Time 00h 56m Time 00 00 2009 05 1 10 05 00 Now Running eee Cycle1 01 of 10 Remainig Time 00h 56m Pause Stop Start View File Skip Interface for system parameter setting Hotlid off Date Time Default User Default File Control Mode Hotlid Key Sound Run End Sound 2009 05 1 10 05 00 IINE N i Yes N Back 1 2 1 Date
44. technology was invented by Mullis in 1985 and thermostable DNA polymerase was discovered by Erlich in 1988 this simple fast specific and sensitive technology has been widely applied to life science areas with astonishing speed Remarkable achievements have been witnessed in cytology virology oncology genetic disease forensic medicine animal plant immunology and etc It is a milestone in contemporary molecular biology development 2 Example of PCR technology application scope Research gene clone DNA sequencing mutation analysis gene recombination and fusion protein regulating DNA sequence identification transposition insertion site mapping genetic modification detection synthetic gene construction clone construction carrier expression certain gene s incision enzyme diversity detection and etc Clinical diagnosis bacteria spirochete mycoplasma Chlamydia mycobacterium rickettsia diphtheria bacillus pathogenic E Coli dysentery bacillus aeromonas hydrophilic and C difficile and etc virus HTLV HIV HBV HCV HPVS EV CMV EBV HSV measles virus rotavirus parvovirus B19 and etc parasite malaria and etc human genetic disease Lesh Nyhan syndrome Mediterranean anemia hemophilia BMD DMD cystic fibrosis and etc Immunology HLA typing T cell receptor or antibody diversity qualification autoimmune disease gene mapping lymphokine quantification and etc Human genome project DNA mark generated from scatt
45. to create new user Computer Enternew username The user name can contain only letters and numbers M OK x Cancel Change user name Select Change user name e Select Change User Name menu under User menu it bounces the dialog box to change user name Input user name and click OK Prompt User name can t be changed if the file under user name is open Change User Name Do you want to change user name of Computer s 123 Enternewusername The user name can contain only letters and numbers vw OK X Cancel User s Manual LifePro Thermal Cycler Connection to computer Copy user Select user name to be copied by device list Select Copy User menu under User menu Paste user Select User sub item in device list or device item in device list Select Paste User menu under User menu Prompt User can t be pasted if copy user is already existed and file is open Paste is only done between local computer and device Delete user 1 Select user to be deleted by device list e 2 Select delete User menu under User menu Prompt User can t be deleted if file under user is open Select local computer Select Import User menu under User menu Select the directory for import click Open Prompt User can t be imported if user to be imported is already existed and user file is open Select user to be exported by local computer Select Export User menu
46. tubes which prevent reagent evaporation and pollution effectively Connect to ordinary computer by RJ45 standard interface which is easy for software upgrading 4 Use of LifePro Thermal Cycler Integrating a number of advanced technologies LifePro Thermal Cycler meets the demand of LifePro Thermal Cycler Introduction different PCR experiments and rank top in similar products LifePro Thermal Cycler is attached with 1 0 2 ml standard centrifugal tube 2 Strip of 8 tube 3 Strip of 12 tube 4 Full half apron plates LifePro Thermal Cycler This chapter introduces the usage transportation storage conditions of the instrument as well as Chapter Il Features its basic parameters performance and functions 1 Caution Normal working conditions Ambient temperature 10 30 Relative humidity lt 70 Power supply 220V 50Hz Before using the instrument please make sure the working condition meets the above requirements Especially the power line must be reliably earthed 2 Transportation and storage conditions 3 Ambient temperature 20 55 Relative humidity lt 80 Basic parameters Specification Sample size TC 96 G H b A 96x0 2ml 96 micro plate 12x8 test tube bar 8x12 test tube bar Scope of temperature 4 99 Heating Rate max gt 4 0 Cooling Rate max gt 4 0 Temperature uniformity lt 0 2 55
47. under User menu Select the directory for export click OK Prompt User can t be exported if user file to be exported is open 3 4 File menu How to 2 Create new file 2 Open and edit file 2 Copy file 2 Paste file 2 Delete file 2 Change file name 2 Change file password 2 Save file 2 Import file 2 Export file Create new file Select user to create file User s Manual LifePro Thermal Cycler Connection to computer Select Create New File menu under File menu to bounce dialog box 3 Input file name and password click OK Create File Do you want to create new file in 1 of Computer Enter new file name The file name can contain only letters and numbers Enter password The password can contain only numbers Enter password again Useempty password Jf OK x Cancel Double click user sub item in device list open file list double click edit file or create new file access the interface to edit file Edit file contents click Save to save file Prompt All steps can be viewed from graphs User must input password to edit file if GenePro password is required by file 28 RG0U8R8 RUM e sl amp o I Computer gt 1 gt 1 10 00 50 00 01 00 01 00 01 00 01 00 01 00 10 00 Begin Segnent 1 5 Seg04 720 E Seg02 550 Seg 3 6
48. ut 0 655535 ms SOK X Cancel Click OK to complete IP address setting Click the COM Mapping in function list User s Manual LifePro Thermal Cycler Connection to computer Network Enabler Administrator COE Mapping File Function COM Mapping View Help COM Mapping 0 COM COM Port Parameter P Address Report add target device 9 remove target device Message Log 5 Monitor Log 0 No Time Description 2008 1 3 14 26 33 Found Network Enabler Module s 1 2008 1 3 14 35 18 Configuration Changed NE 4110S 00 30 E8 10 E2 B7 2008 1 3 14 35 39 Configuration Changed NE 4110S 00 90 8 10 2 7 2008 1 3 14 35 49 Configuration Changed NE 4110S 00 90 8 10 2 7 2008 1 3 14 37 22 Configuration Changed NE 4110S 00 90 8 10 2 7 Hetwork Enabler Administrator CON Mapping File Function COM Mapping View Help DOMSA Function COM Mapping 1 COM Po Network Enabler Admit i IP Address COM Port Mode Configuration Mapping Monitor Port Monitor XE IP Address Report Message Log 5 Monitor Log Description 2008 1 3 14 26 33 Found Network Enabler Module s 1 2008 1 3 14 35 18 Configuration Changed NE 41105 00 90 8 10 2 87 2008 1 3 14 35 39 Configuration Changed 41105 00 90 8 10 2 7 2008 1 3 14 35 49 Configuration Changed 41105 00 90 E8 2008 1 3 14
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