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N07848_ver2.5 MagMAX Express User Manual

1. 1 Warning Ensure that the mains switch Fig 4 3 on the bottom left of the back Connect the instrument to a correctly installed line power outlet which has a protective conductor that is grounded Warning Never operate your instrument from a power outlet that has no ground connection Fig 5 3 Connecting the mains supply cable 5 3 Operational check 1 First switch the instrument ON The instrument performs initialization tests and adjustments The initialization tests are so called self tests 2 The display quickly shows the internal software version and after that the display shows one of the protocols in the internal memory This happens when the initialization tests and adjustments have been completed 3 We recommend that you carry out an empty run for example a demonstration to verify proper instrument operation Applied Biosystems MagMAX Express User Manual Rev B June 2011 20 Chapter 6 Routine Operation 6 Routine Operation 6 1 Switching on Before you switch on the MagMAX Express ensure that the voltage on the rating label at the bottom left of the back panel Fig 4 3 corresponds to the local voltage 6 2 How to use the keypad and display The liquid crystal display is a 20 character by 2 row line LCD The lower line of the display can show the steps of the protocol while the processing is running Furthermore if errors occur they will be displayed on the lower row The keypad and
2. CAUTION Remove Transport Lock Before Use is fastened to the instrument to attract the user s attention Fig 5 7 The tag in Fig 5 7 and the black arrows in Fig 5 2 indicate where the transport lock piece is situated Remove the tag and release the transport lock Ensure that the transport lock has been released before you put the instrument into operation Applied Biosystems MagMAX Express User Manual Rev B June 2011 18 Chapter 6 Routine Operation Fig 5 1 MagMAX Express transport lock tag present before release of the lock piece 1 To remove the transport lock unscrew the three screws Fig 5 2 with the 2 5 mm hexagonal screwdriver supplied 2 Remove the transport lock piece 3 Save the transport lock piece and screws and the transport lock warning tag for future transportation Tag attached here Fig 5 2 Location of the transport lock screws Applied Biosystems MagMAX Express User Manual Rev B June 2011 19 Chapter 6 Routine Operation 5 2 2 How to ensure the startup panel is in the OFF position 2 Note Connect the mains supply cable to the ma ns power socket Fig 5 3 at the bottom left of the back panel If any other type of mains supply cable than supplied is needed use only cables certified by the local authorities Before you plug in the power cable ensure that the voltage on the rating label at the bottom left of the back panel Fig 4 3 corresponds to the local voltage
3. 8 data bits 1 stop bit no parity Flow control XON XOFF Performance specifications Microplate type disposable Magnetic rods fixed Capacity Vessel volumes Vessel type disposable Tip comb disposable Magnetic particle size Collecting efficiency Incubating temperature Keyboard Display Software Special design 8 x 12 grid format 2 x 12 format 24 samples run 2 x 12 20 200 ul Special microstrip 1 x 8 wells Special design 1 x 12 format ca gt 1 um 99 vessel washing buffer Ambient temperature i e no incubation START STOP two cursor keys LCD 1 8 fixed internal protocols loading through the external PC KingFisher Software can be used to create and modify protocols Applied Biosystems MagMAX Express User Manual Rev B June 2011 28 Chapter 10 Warranty Information 8 2 Safety specifications The MagMAX Express fulfills the following requirements EN 61010 1 1993 A2 1995 IEC 61010 1 1990 A1 1992 A2 1995 The safety specifications are also met under the following environmental conditions in addition or in excess to those stated in the operating conditions Altitude Up to 2000 m Temperature 5 C 40 C Mains supply fluctuations 10 from nominal Installation category overvoltage category ll according to IEC 60664 1 see Note 1 Pollution degree 2 according to IEC 60664 1 see Note 2 o Note 1 The installation ca
4. A3 The MagMAX Express magnets are made of material that is very stable The magnetic field will not get weaker Magnets can be replaced if needed Usually there is no need to replace them but it can be done for example if they are mechanically damaged during runs for example the plate is not correctly positioned during the run Changing the magnets can be carried out by local technical service personnel O4 How strong are the magnets Can they disturb for example some sensitive equipment A4 The magnetic field is situated just around the magnetic tips so it is very local Therefore the magnetic field should not cause any problems with surrounding equipment However if you touch your floppy disk with the magnetic rod you will lose all your data Generally a normal PC screen may also detect the magnetic field with a minor distortion of the image Q5 What happens if forget to insert the tip combs into the tip comb holder and the magnetic particles stick to the magnetic rods Ab Wipe the magnetic rods with a soft cloth or tissue paper soaked in a mild detergent solution soap solution or alcohol Q6 What if magnetic particles remained in the sample well A6 If the starting material is too viscose the magnetic rods will not be able to collect the particles Dilute the sample and check that the sample is properly homogenized lysed Q7 What if magnetic particles are attached to the tip combs A7 This happens som
5. Fig 4 2 Closed lids protect the processing against environmental contamination The operating principle employed is MPP nverse magnetic particle processing technology Fig 4 6 Rather than moving the liquids the magnetic particles are moved from microwell to microwell containing specific reagents in contrast to the external magnet method Magnetic particles are transferred with the aid of magnetic rods covered with disposable specially designed plastic tip combs AB EE Ses Fig 4 5 MagMAX Express magnetic particle processor Applied Biosystems MagMAX Express User Manual Rev B June 2011 14 Chapter 4 Functional Description 4 3 Principle of the MagMAX Express magnetic particle processor Magnetic rod uw Transfer Tip XXX P i Magnetic particle Collection of Magnetic particles Release of Ready suspension before magnetic particles on the tip surface magnetic particles suspension collection Fig 4 6 Inverse magnetic particle processing 4 4 Working with a magnetic rod Working with magnetic particles can be divided into five separate processes collecting particles releasing particles washing particles incubation concentration 4 4 1 Collecting magnetic particles During the collection of the magnetic particles the magnetic rod bar is fully inside the tip The magnetic rods together with the tip comb s move slowly up
6. If for any reason it becomes necessary to return material to Applied Biosystems contact Applied Biosystems Technical Support or your nearest Applied Biosystems subsidiary or distributor for a return authorization RA number and forwarding address Place the RA number in a prominent location on the outside of the shipping container and return the material to the address designated by the Applied Biosystems representative Applied Biosystems MagMAX Express User Manual Rev B June 2011 38 Chapter 12 Glossary and Abbreviations 11 Ordering Information Code Instrument System 4387987 MagMAX Express 100 240 V 11 1 List of accessories Code Item Quantity N07848 MagMAX Express User Manual 1 4388452 MagMAX Express Tip Comb 50 pcs box 4388474 MagMAX Express Plate 200 50 pcs box 11 2 List of spare parts Code Item Quantity 11272711 Plate tray 1 2305290 Serial cable F9 F25 for RS 232C port 1 5186980 KingFisher Software 1 11 3 List of recommended spare parts Code Item 1 2 unit s year 10 units year 21040820 H motor assembly 21040850 M motor assembly 24070740 Free wheel assembly 1 24072290 Magnetic plate assembly 1091910 Tooth belt T2 5 330 1 1092250 Tooth belt T2 5 265 1 2004460 PCB PSP 01 1 2004470 PCB CSU 02 1 2 2004480 PCB CSU 01 1 21040811 Power supply 1 2 1 1 3 1 Contact the Applied Biosystems technical service department Applied Biosystems MagMAX Express User Manual Rev
7. 1 or 2 Instruments exposed to biological agents in Hazard Group 1 or 2 situated in a Biosafety Level Containment Level 1 or 2 laboratory or exposed to any human source material or cell culture including clinical samples a Follow Universal Precautions and wear all applicable personal protective equipment Place the instrument in a well ventilated area b Apply a freshly made solution of 1 10 v v dilution of 5 25 sodium hypochlorite e g Clorox bleach with water to accessible product surfaces A spray dispenser may help to ensure complete coverage C Keep surfaces wet for at least 15 minutes then wipe dry d Repeat steps b and c e Clean all decontaminated surfaces with water to remove residual bleach and wipe dry If an alternative to 1 10 v v dilution of bleach is used for instrument decontamination please provide details in written form and attached to the Certificate of Instrument Decontamination Hazard Group 3 or 4 Instruments situated in Biosafety Level Containment Level 3 or 4 laboratories must be decontaminated by the customer using an internationally approved sterilization procedure The customer must then move the instrument to either a Containment Level 1 or 2 laboratory for service Applied Biosystems employees are not permitted to enter Biosafety Level Containment Level 3 or 4 laboratories It may not be possible for Applied Biosystems to service or transport these instruments 2 Radioactive Materials Ap
8. 232C ZS GA NM Fig 4 3 MagMAX Express rear view 4 1 3 Internal view ea wull Plate carrier Tip comb holder Tip comb slot Fixed magnetic rod holder P GA NM Fig 4 4 MagMAX Express internal view Applied Biosystems MagMAX Express User Manual Rev B June 2011 13 Chapter 4 Functional Description 4 2 MagMAX Express magnetic particle processor The MagMAX Express magnetic particle processor Fig 4 5 is designed for maximum two 96 well plates and special microstrips which are compatible with the tip comb The plate s is are kept stationary and the only moving assembly is the processing head with tip combs and magnetic rods The head consists of two vertically moving platforms One is needed for the magnetic rods 2 x 12 pieces and the other one for the plastic tip combs Fig 4 4 One plate contains twelve separate microstrips and one sample processing uses one microstrip containing eight wells One tip comb containing twelve tips is used for processing twelve samples at a time within one plate Before starting the magnetic particle processing via the keypad and display the samples and reagents are dispensed into the wells and the tip comb s is are loaded into its their slot s The plate s is are placed onto the moving plate carrier in the correct position and the plate carrier is pushed into the end position During the operation the front and top lids can be closed Fig 4 7 or open
9. B June 2011 39 12 Glossary and Abbreviations Chapter 12 Glossary and Abbreviations Frame The frame where the special microstrips are placed Magnetic rod The rods which are magnetic and collect magnetic particles The rods do not collect the particles on their own the magnetic rods must always be protected by a tip comb Microstrip The wells where all the reagents and samples are located and where the or microwell strip processing takes place Plate Frame microstrips 12 pieces Tip comb Protects the magnetic rod A tip comb always has to be fitted into the tip comb holder slot during processing 12 1 Keywords for web pages affinity separation amplification Applied Biosystems beads cloned DNA cloning DNA DNA complex DNA immobilization DNA purification DNA strand separation DNA template double stranded DNA dsDNA encapsulated magnetic microspheres genome genomic DNA hybride selection MagMAX Express magnetic beads magnetic microspheres magnetic particles magnetic separation microspheres nucleotide oligo dT particles PCR PCR amplification PCR product post PCR purification primer probes processing processor purification RNA mRNA RT PCR separation separator silica coated beads silica coated microspheres silica coated particles streptavidin coated beads streptavidin coated microspheres streptavidin coated particles subtraction libraries superpa
10. Serial Number AB Instrument Description Reason for return service RMA or RA Number if required Decontamination Information and Procedures 1 Biological Agents Has this instrument been exposed to viable biological agents YES o NO o If YES please state the viable biological agent s their Hazard Group s and Biosafety Level Category of Containment If YES please describe the procedures used to decontaminate the instrument 2 Radioactive Materials Has this instrument been exposed to radioactive materials YES o NO o If YES please identify the radioactive isotopes If YES please describe the procedures used to decontaminate the instrument If YES are the radioactivity survey results at or below local background level YES o NO o Attach the results to this form orin the US only for service work excluding any transportation at levels designated to be clean or safe as stated in the Customer s regulatory approved Site Radioactive Materials License 3 Hazardous Chemicals Has this instrument been exposed to chemicals that are very toxic in quantities harmful to human contact carcinogenic mutagenic toxic for reproduction sensitizing and or which have not yet been fullytested YES D NO D If YES please identify the hazardous chemicals If YES please describe the procedures used to decontaminate the instrument The Customer understands and agrees that decontamination is critical to issues of health and safety
11. and that thoroughly completing this Certificate is essential Customer represents and warrants to Applied Biosystems that it performed all decontamination procedures as described in this Certificate and completed this Certificate accurately truthfully and in full Customer hereby assumes all responsibility and liability for and shall defend and indemnify Applied Biosystems against injury or damage of whatever kind incurred by Applied Biosystems its employees contractors and or agents that result directly or indirectly from Customer s breach of this representation and warranty The Customer accepts that Applied Biosystems has no obligation to repair service or transport any product if this Certificate is not completed in full Name Title Company Phone Signature Date Page 1 of 2 AB Part No 4343055 rev D Applied Biosystems MagMAX Express User Manual Rev B June 2011 46 Appendix A Certificate of Instrument Decontamination Applied Bibsystems Examples of Instrument Decontamination Procedures 1 Biological Agents The following procedures are based on widely used industry standards for instrument or part hereinafter referred to as instrument decontamination but the Customer must assess the suitability of these methods for the biological agents concerned Some instruments may require decontamination by an outside service refer to your User Manual or Applied Biosystems Representative for further information Hazard Group
12. display are shown in Fig 6 7 Le Applied AB Biosystems MagMAX Express Fig 6 1 MagMAX Express keypad The keys available are two cursor keys or arrow keys direction keys to select the processing step to be run To change to the previous next protocol by scrolling up x To change to the next protocol by scrolling down two function keys START To initiate the processing step STOP To pause terminate the processing step In short STOP paused START the instrument continues after a Pause step STOP paused STOP the processing is terminated Applied Biosystems MagMAX Express User Manual Rev B June 2011 21 Chapter 6 Routine Operation 6 3 How to START e First of all slide the plate carrier out Fig 6 2 mE Y E m SS K Fig 6 2 Loading the plate carrier e Load only maximum two special plates Fig 6 5 onto the plate carrier for processing at a time The plates have to be positioned so that the cut corners face left down Fig 6 2 They also have to be in their places Slide the plate carrier fully back in AB GEET Cut corner Fig 6 3 Positioning of the plate s and insertion of the tip comb s Applied Biosystems MagMAX Express User Manual Rev B June 2011 22 Chapter 6 Routine Operation e The tip comb s always has have to be in its their slot s and fully inserted Fig 6 3 The instrument also functions with either one microplate or one to twelve mi
13. nia due ra dad dude eegend 8 17 39 49 V MI 12 13 NECARE 12 ld 13 PR A A A A A AAA AE A A ee tege 13 Me CEET 18 20 21 28 30 SOLO CUO E tia ii li EE a dea da 20 21 W WV ATI GS TEEN 18 19 20 31 WA ANOVA A DA A Ac Cera edd a 8 17 35 Wells ES 18 28 Applied Biosystems MagMAX Express User Manual Rev B June 2011 44 Chapter 14 Table of Figures 14 Table of Figures Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig 3 1 MagMAX Express magnetic particle prOCOSS OT seen 10 4 1 MagMAX Express front view with lids closed 12 4 2 MagMAX Express front view with lids open 12 4 3 MagMAX Express rear Wie 13 4 4 MagMAX Express internal wie 13 4 5 MagMAX Express magnetic particle prOCOSS OT eene nnn 14 4 6 Inverse magnetic particle Groceseimg esee nasse nnn 15 4 7 Concentration step during magnetic particle processing 16 5 1 MagMAX Express transport lock tag present before release of the lock piece s 19 5 2 Location of the transport lock SCreWs sss seinen enenatis nnns 19 5 3 Connecting the mains supply cable s dtr ot n ae a eet 20 6 1 Mag MAX Express keypad EE 21 6 2 Loading INE plate Cartier uoc e ER ERLEBT DEEP rea 22 6 3 Positioning of the plate s and insertion of the tip combtsl nens 22 6 4 Tip comb specially designed for the MagMAX Express 23 6 5 Plate con
14. the procedure recommended below or some other corresponding decontamination procedure must be performed We strongly recommend that the complete decontamination procedure is performed before relocating the instrument from one laboratory to another Decontamination is not required for the proper functioning of the instrument Example of decontaminants Ethanol 70 Virkon solution 1 3 Glutaraldehyde solution 4 Chloramine T Microcide SO 1 64 Warning Always use disposable gloves and protective clothing and operate in a well ventilated area 1 Prepare the decontaminant for example 200 ml 4 glutaraldehyde solution or another agent recommended by your safety officer 2 Empty the plate carrier Ensure that you are wearing disposable gloves 3 Switch OFF the power and disconnect the mains supply cable Fig 5 3 4 Disinfect the outside of the instrument using a cloth dampened with 70 ethanol 5 Place the instrument in a large plastic bag Ensure that the lids are open 6 Place a cloth soaked in the prepared solution into the bag Ensure that the cloth does not make contact with the instrument 7 Close the bag firmly and leave the instrument in the bag for at least 24 hours 8 Remove the instrument from the bag 9 Clean the instrument using a mild detergent Applied Biosystems MagMAX Express User Manual Rev B June 2011 26 Chapter 7 Maintenance 10 Remove any stains using 70 ethanol 11 After performing t
15. Applied Biosystems MagMAX Express User Manual Rev B June 2011 47 Appendix B MagMAX Express Brief User s Guide 16 Appendix B MagMAX Express Brief User s Guide e Switch the MagMAX Express on p 20 e Pipette the samples reagents and washing buffers into the microwells of the plate s p 46 e Load the plate s onto the plate carrier p 22 e Insert the tip comb s into the tip comb holder slot s p 22 e Select the desired protocol from the keypad and display p 21 e Press the START button p 22 e After the end of the run remove the plate and tip combs p 24 e Maintain your MagMAX Express instrument regularly p 25 IS Applied A Bibtystems MagMAX Express el Fig 16 1 MagMAX Express keypad Applied Biosystems MagMAX Express User Manual Rev B June 2011 48 Appendix C MagMAX Express Feedback Form Please send to Applied Biosystems Fax 512 651 0201 17 Appendix C MagMAX Express Feedback Form Cat no Serial no PURCHASED BY PURCHASED FROM Company Institute Distributor Department Address Address Tel Tel Fax Date of delivery Internet home page Date of purchase Your research area Dr O Mr D Mrs O Ms O Job title Position Surname block capitals First name block capitals Internet e mail address Excellent Above As Below Comments expectations expected expectations Reagent kit Instructions D O O L1 Instrument User manual D O L1 O Operat
16. B June 2011 10 Chapter 3 Introduction to the MagMAX Express 3 3 Advantages of processing magnetic particles with the MagMAX Express The MagMAX Express magnetic particle processor provides several advantages relating mainly to the principle of operation in that it Enables automation of complicated manual steps Enables simultaneous processing and purification for example of double stranded DNA Enables a quicker reaction and a more efficient wash due to the simultaneous shaking during incubation and washing Facilitates a good collection of bead bound sample due to the efficiency of the magnet Prevents carry over due to the fact that the same tip is only used for one sample and furthermore that the reagents have been pipetted into their own wells different reagents are not added into the same well Is easy to carry out the whole processing with the aid of an internal program Applied Biosystems MagMAX Express User Manual Rev B June 2011 11 Chapter 4 Functional Description 4 Functional Description 4 1 Instrument layout 4 1 1 Front view 1 Keypad 2 Display 3 Front lid 4 Top lid Fig 4 1 MagMAX Express front view with lids closed Fig 4 2 MagMAX Express front view with lids open Applied Biosystems MagMAX Express User Manual Rev B June 2011 12 Chapter 4 Functional Description 4 1 2 Back view ON OFF switch Mains power supply socket Cooling air outlet Serial port RS
17. ED BIOSYSTEMS WILL NOT BE BINDING ON APPLIED BIOSYSTEMS UNLESS IN A WRITING SIGNED BY AN EXECUTIVE OFFICER OF APPLIED BIOSYSTEMS THIS WARRANTY IS LIMITED TO THE BUYER OF THE PRODUCT FROM APPLIED BIOSYSTEMS AND IS NOT TRANSFERABLE Some countries or jurisdictions limit the scope of or preclude limitations or exclusion of warranties of liability such as liability for gross negligence or willful misconduct or of remedies or damages as or to the extent set forth above In such countries and jurisdictions the limitation or exclusion of warranties liability remedies or damages set forth above shall apply to the fullest extent permitted by law and shall not apply to the extent prohibited by law Damages Claims and Returns Damages If shipping damage to the product 1s discovered contact the shipping carrier and request inspection by a local agent Secure a written report of the findings to support any claim Do not return damaged goods to Applied Biosystems without first securing an inspection report and contacting Applied Biosystems Technical Support for a Return Authorization RA number Applied Biosystems MagMAX Express User Manual Rev B June 2011 37 Chapter 10 Warranty Information Claims After a damage inspection report is received by Applied Biosystems Applied Biosystems will process the claim unless other instructions are provided Returns Do not return any material without prior notification and authorization
18. ER i FO D Ried ee ethene 12 4 1 1 Eront VIEW dere c n bep ette ee acea ENNER 12 4 1 2 Back view ot nig b CENE REOR PENIS 13 4 1 3 Internal Vie HER 13 42 MagMAX Express magnetic particle Drocessor nc nn rn nn nn 14 43 Principle of the MagMAX Express magnetic particle processor ccconccccnonnnnnncnannnncnananns 15 4 4 Working with a magnetic rode 15 4 4 1 Collecting magnetic parttcles eene nnne nnne 15 4 4 2 Releasing magnetic particles AAA 15 4 4 3 Washing magnetic particles enne nnne n nnne nennen nns 16 4 4 4 lnGubation A A ees eee 16 4 4 5 Changing the volume during the magnetic particle processing sssss 16 5 SE AAA Ee ENEE 17 5 1 What to do upon Celivery cc tina dia CERE 17 5 1 1 How to NIT 17 5 1 2 Checking delivery for completeness nennen nnne 17 5 1 3 Checking for damage during transport 17 5 1 4 Environmental requirements eene nennen ennt nnn nene nne 17 5 1 5 THINGS to aVoir EE 18 5 1 6 Technical prere quisites itr rrr ti 18 5 2 Setups before you put the instrument into operation ooooccconccnnccnanonnnonaninnncnannnnnnrnnnnannnnnnnss 18 5 2 1 How to release the transport lock sess nnn 18 5 2 2 How to ensure the estartup ENEE EEN 20 5 32 Operational Check xr A o di es 20 6 Routine Operation iii 21 GU AS Wit MING OM ies ooo rto rire E pere a cee 21 6 2 How to use the keypad and deplay enne 21 6 3 Howto START edel ee e vedi a e t LE des A e a dep Ud YE nex
19. Era 22 Ga SPOON EE 24 Applied Biosystems MagMAX Express User Manual Rev B June 2011 4 Table of Contents AAPP TS 25 7 1 Regular and preventive maintenance sess esee eene nnn enne nnn nen 25 7 1 1 How to clean the plate carier nennen nnne enn nnn 25 7 1 2 How to clean the magnetic rode 25 7 1 3 How to clean the processing chamber 26 7 2 Disposal of materials reete xen eR ped XR ERR e dua x Pee Reg 26 7 3 Decontamination Drocedure seen enne en nene n entrer sees nn nennen nns 26 7 4 Howto pack for service aei teniente een rante ee nnn enn Ee Een n Ri Re YR en a 27 Zb Service C NTAC S Sinear e e ee na 27 7 6 Disposal of the mnstrument eene nnne nnne nnn nennen nennen entren enne nns 27 8 Technical Specifications aa 28 8 1 General specifications oooocccnonnccccnnnnocncnnanonnnnnnnonononnnno nn nn naar a nr nnne nnne nn rr naar nnne ener 28 8 2 Satety Specifications vdd de FORTE PERO ad 29 8 3 In conformity with the requirements oooocccccnnnonccnnnnonnnnnanannnonnnnonnnnnnno nn nn nnnnr arc n naar are enne nnn 30 9 Troubleshooting Guide iii ia aaa 31 9 1 Error messages and warnings a aeee aaa cnn enano rr rre 31 9 2 Troubleshooting Guide eiii SEENEN SEENEN Ee HERR 32 9 3 Frequently asked questions FAQ about the MagMAX Express eene 33 9 4 Service request Drotocol ener nnne nnn enne ener nnne nnn 34 10 Warranty ge E 2 i erteeterei decere pace aE D p
20. HE REMEDIES PROVIDED HEREIN ARE THE BUYER S SOLE AND EXCLUSIVE REMEDIES WITHOUT LIMITING THE GENERALITY OF THE FOREGOING IN NO EVENT SHALL Applied Biosystems MagMAX Express User Manual Rev B June 2011 36 Chapter 10 Warranty Information APPLIED BIOSYSTEMS BE LIABLE WHETHER IN CONTRACT TORT WARRANTY OR UNDER ANY STATUTE INCLUDING WITHOUT LIMITATION ANY TRADE PRACTICE UNFAIR COMPETITION OR OTHER STATUTE OF SIMILAR IMPORT OR ON ANY OTHER BASIS FOR DIRECT INDIRECT PUNITIVE INCIDENTAL MULTIPLE CONSEQUENTIAL OR SPECIAL DAMAGES SUSTAINED BY THE BUYER OR ANY OTHER PERSON OR ENTITY WHETHER OR NOT FORESEEABLE AND WHETHER OR NOT APPLIED BIOSYSTEMS IS ADVISED OF THE POSSIBILITY OF SUCH DAMAGES INCLUDING WITHOUT LIMITATION DAMAGES ARISING FROM OR RELATED TO LOSS OF USE LOSS OF DATA FAILURE OR INTERRUPTION IN THE OPERATION OF ANY EQUIPMENT OR SOFTWARE DELAY IN REPAIR OR REPLACEMENT OR FOR LOSS OF REVENUE OR PROFITS LOSS OF GOOD WILL LOSS OF BUSINESS OR OTHER FINANCIAL LOSS OR PERSONAL INJURY OR PROPERTY DAMAGE NO AGENT EMPLOYEE OR REPRESENTATIVE OF APPLIED BIOSYSTEMS HAS ANY AUTHORITY TO MODIFY THE TERMS OF THIS LIMITED WARRANTY STATEMENT OR TO BIND APPLIED BIOSYSTEMS TO ANY AFFIRMATION REPRESENTATION OR WARRANTY CONCERNING THE PRODUCT THAT IS NOT CONTAINED IN THIS LIMITED WARRANTY STATEMENT AND ANY SUCH MODIFICATION AFFIRMATION REPRESENTATION OR WARRANTY MADE BY ANY AGENT EMPLOYEE OR REPRESENTATIVE OF APPLI
21. MagMAX Express User Manual Rev BX ES G ed ms O Copyright 2008 2011 Applied Biosystems All rights reserved Information in this document is subject to change without notice Applied Biosystems assumes no responsibility for any errors that may appear in this document APPLIED BIOSYSTEMS DISCLAIMS ALL WARRANTIES WITH RESPECT TO THIS DOCUMENT EXPRESSED OR IMPLIED INCLUDING BUT NOT LIMITED TO THOSE OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE IN NO EVENT SHALL APPLIED BIOSYSTEMS BE LIABLE WHETHER IN CONTRACT TORT WARRANTY OR UNDER ANY STATUTE OR ON ANY OTHER BASIS FOR SPECIAL INCIDENTAL INDIRECT PUNITIVE MULTIPLE OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR ARISING FROM THIS DOCUMENT INCLUDING BUT NOT LIMITED TO THE USE THEREOF Limited Use Label License Veterinary Diagnostics The purchase of this product conveys to the purchaser the limited non transferable right to use the purchased amount of the product only to perform veterinary diagnostic services including reporting the results of purchaser s activities for a fee and for the purchaser s own internal research No right to resell this product or any of its components is conveyed expressly by implication or by estoppel For information on obtaining additional rights please contact outlicensing lifetech com or Out Licensing Life Technologies 5791 Van Allen Way Carlsbad California 92008 No right under any other patent claim such as claims to methods a
22. ONS EE 28 Bet E 30 RI Waler 24 25 26 27 34 Disposal of insti rrietit io rada aia ia tilda izo fas 27 Disposal of materials cocido ceret tree Ec edere cere dte Wien ee redet eee Eua rt Bee 26 RIVE 11 23 33 40 41 E Environmental requirements ida gate t dc A e de de ED tuno 18 Error unt EE 8 31 F EA A A A A EE A A A A Sada abateaitiitea bed AA 33 FeegdbaekEotm i a e en Deeg EE e 17 49 Frame n Eeer tee eege EE a ge EE ege e EE 32 40 Frequently asked We EE LTE 33 G GIOSSARY E 40 H How to clean the magnetic rode 25 How to clean the plate carier ENEE 25 How to clean the processing chamber 25 How to disinfect the Instrument 24 25 26 34 How to ensure startup e pr EE ERO DP RENE ee aes eme EE MERE 22 How to install the MagMAX Express coomcccccocccoconncononononcnnnnnnnnn eee nn cnn cnn rre 17 29 How to maintain the instrument esses nnnennn nnn nenish sina sn sn rr 2b How to packfor service hene Fede edes iei tenete t aee tet eee mute ere cea aee ede ovde a a a qa x 27 34 Applied Biosystems MagMAX Express User Manual Rev B June 2011 42 Chapter 13 Index How to release the transport lock ee 18 How to setup the instrument before you put it into operation nnn 18 Howto SHUT WR dmca erre Depende dE DEEN dee debe dae ebria Pelr dr PR P T e 24 HOWTO SWITCH D iiec irai ae irren cr Feo en ha bn eed em eee raa Fs wa Fade voe EY a oe R a Ce ah Cre Ea Pn dia 21 23 How to troubleshoot the ms
23. ailable in the Control Console IMPORTANT To verify your client connection to the database you need a valid user ID and password 2 1 6 Safety Alert Words Safety alert words also appear in user documentation For more information see Safety Symbols and Markings on page 6 2 2 How to Obtain More Information 2 2 1 Related Documentation The following related documents are shipped with the system e Applied Biosystems MagMAX Express User Guide Describes the Applied Biosystems MagMAX Express hardware and software and provides information on preparing maintaining and troubleshooting the system e Applied Biosystems MagMAX Express Service Guide Provides explanatory procedures for servicing the Magnetic Particle Processors 2 3 How to Obtain Support For the latest services and support information for all locations go to http www appliedbiosystems com then click the link for Support At the Support page you can e Search through frequently asked questions FAOs e Submit a question directly to Technical Support e Order Applied Biosystems user documents MSDSs certificates of analysis and other related documents e Download PDF documents e Obtain information about customer training e Download software updates and patches In addition the Support page provides access to worldwide telephone and fax numbers to contact Applied Biosystems Technical Support and Sales facilities Applied Biosystems MagMAX E
24. and down in the microwells and the magnetic particles are collected onto the wall of the tips The magnetic rods together with the tip comb s having collected the magnetic particles can be lifted out of the microwells and transferred into the next microwells 4 4 2 Releasing magnetic particles After collection of the magnetic particles the magnetic rods together with the tip comb s are lifted from the microwells the magnetic rods are lifted off and the tip comb s is are lowered into the next microwells containing a reagent Magnetic particles are released by moving the tip comb s up and down several times with considerably high speed until all the particles have been mixed with the substance in the next reaction Applied Biosystems MagMAX Express User Manual Rev B June 2011 15 Chapter 4 Functional Description 4 4 3 Washing magnetic particles Washing the magnetic particles is a frequent and an important processing phase Washing is a combination of the release and collection processes in a microwell filled with washing solution To maximize washing efficiency the magnetic rods together with the tip comb s are designed to have minimized liquid carrying properties 4 4 4 Incubation To keep the magnetic particle suspension evenly mixed in long running reactions the tip comb s can be moved up and down from time to time 4 4 5 Changing the volume during the magnetic particle processing The volume of the first micr
25. be cleaned with a mild laboratory detergent or alcohol Warning If any surfaces have been contaminated with biohazardous material a mild A sterilizing solution should be used Note Do not autoclave any part of this instrument 7 1 1 How to clean the plate carrier Keep the plate carrier surface clean to avoid dust and dirt entering into the instrument Clean the plate carrier surface at least once a week using a soft cloth or tissue paper soaked in a mild detergent solution SDS soap solution or alcohol If you have spilt infectious agents on the plate carrier disinfect according to Section 7 3 Decontamination procedure 7 1 2 How to clean the magnetic rods If required clean the magnetic rods using a soft cloth or tissue paper soaked in a mild detergent solution SDS soap solution or alcohol Applied Biosystems MagMAX Express User Manual Rev B June 2011 25 Chapter 7 Maintenance 7 1 3 How to clean the processing chamber If required clean the processing chamber using a soft cloth or tissue paper soaked in a mild detergent solution SDS soap solution or alcohol 7 2 Disposal of materials Refer to local regulations for the disposal of infectious material Warning The samples can be potentially infectious Dispose of all used microplates AN disposable gloves syringes disposable tips etc as biohazardous waste 7 3 Decontamination procedure If there is any risk of contamination with biohazardous material
26. c particle processor including installation and operating instructions Read the manual in its entirety prior to operating the instrument This user manual has been designed to give you the information you need to e Install the MagMAX Express e Use the MagMAX Express in routine the processing step e Perform basic maintenance procedures This user manual also describes all the features and specifications of the MagMAX Express hardware and on board In Chapter 9 Troubleshooting Guide you will find explanations of all error messages and a problem solving guide The user should be familiar with the contents of Chapter 7 on maintenance For warranty and ordering information refer to Chapters 10 Warranty Information and 11 Ordering Information In an effort to produce useful and appropriate documentation we appreciate your comments on this document to your local Applied Biosystems representative 2 1 Preface 2 1 1 Purpose of This Guide The Applied Biosystems MagMAX Express User Guide provides reference material related to the Applied Biosystems MagMAX Express Magnetic Particle Processor It describes the system theory of operation and how to prepare maintain and troubleshoot the system 2 1 2 Audience This guide is intended for novice and experienced MagMAX Express users who will e Install the Applied Biosystems MagMAX Express e Perform routine runs using the Applied Biosystems MagMAX Express
27. crostrips depending on the amount of samples At least one tip comb Fig 6 4 always has to be inserted into either of the narrow slots meant for the tip comb Fig 6 4 Tip comb specially designed for the MagMAX Express e Close the front lid if you want to The front lid protects the instrument against environmental contamination Note The front lid and or top lid can be left open if desired This action does not break 1 the run e Select the name of the method for example Genomic DNA 1 e Use the START key to initiate the run For startup refer to Section 6 1 Switching on Note The samples are collected immediately after the run and transferred into other 1 tubes for longer storage A AAA 4 ote 4224 Fig 6 5 Plate containing microstrips specially designed for the MagMAX Express Applied Biosystems MagMAX Express User Manual Rev B June 2011 23 Chapter 6 Routine Operation 6 4 Shutdown Switch the MagMAX Express off by pressing the power switch Fig 4 3 at the bottom left of the back panel of the instrument into the OFF position Warning Remove any plate still on the plate carrier Dispose of all microplates as A biohazardous waste Warning Remove any tip combs still in the instrument Dispose of tip combs as A biohazardous waste Wipe the plate carrier surface and the neighboring instrument surface with a soft cloth or tissue paper moistened with distilled water a mild detergen
28. d Olsvik Advances in Biomagnetic Separation Eaton Publishing Pp 61 71 Lambert K N and Williamson V M 1993 DNA library construction from small amounts of RNA using paramagnetic beads and PCR Nucleic Acids Res 21 775 776 Lee Y H and Vacquier V D 1992 Reusable cDNA libraries coupled to magnetic beads Anal Biochem 206 206 207 Rudi K Kroken M Dahlberg O J Deggerdal A Jakobsen K S and Larsen F 1997 Rapid universal method to isolate PCR ready DNA using magnetic beads BioTechniques 22 506 511 Sinclair B 1998 To bead or not to bead Applications of magnetic bead technology Scientist 12 16 19 Applied Biosystems MagMAX Express User Manual Rev B June 2011 41 Chapter 13 Index 13 Index A tele LEE 40 About BET EE 8 ACCESS OMIES a Fed el mad dai E 17 39 Ad iia 50 Advantages of processing magnetic Darticles EE 11 ADPIC ION iii di A a AA A Hr 6 33 41 49 B Brief WSO SGU Ae Eden deen ea a AR d 48 C Cable ati Ga ee e Eegen gege Ee 20 26 39 Certificate of DECONTAMINATION ooonononnnnnnnnnnnnnononananananononan aran n nn ano nnknu unuunu nine tis a daas assa nada dana dada 27 34 46 erm 17 20 32 34 EST EE 18 25 26 33 magtietic OS A DUE ERR 25 processing Et un LEE 25 Concentration tds a Fh re a KENE aii baaa 15 16 D DECONTAMINATION oi cia a A a ca e dy tc ad Fr un gs 27 34 46 Certificate nocilla Fla LEE Fra re ETE ed Ra DU o dre a ides 27 34 46 DIMENSI
29. d documentation provided by the manufacturer for future use 5 1 2 Checking delivery for completeness Check the enclosed packing list against order If any parts are missing contact your local Applied Biosystems representative or Applied Biosystems 5 1 3 Checking for damage during transport Visually inspect the transport package the instrument and the accessories for any possible transport damage If the carton has been damaged in transit it is particularly important that you retain it for inspection by the carrier in case there has also been damage to the instrument Visually check all interconnections in the basic instrument Check that there are no loose parts inside the instrument If any parts are damaged contact your local Applied Biosystems representative or Applied Biosystems 5 1 4 Environmental requirements When you set up your MagMAX Express avoid sites of operation with excess dust vibrations strong magnetic fields direct sunlight draft excessive moisture or large temperature fluctuations e Make sure the working area is flat dry clean and vibration proof and leave additional room for cables lids etc e Make sure the ambient air is clean and free of corrosive vapors smoke and dust e Make sure the ambient temperature range is between 10 C 50 F and 40 C 104 F Applied Biosystems MagMAX Express User Manual Rev B June 2011 17 Chapter 6 Routine Operation e Make sure relative humidit
30. d maintenance items replaceable products or components used on or in the instrument are themselves warranted to be free of defects in materials and workmanship for a period of ninety 90 days Applied Biosystems MagMAX Express User Manual Rev B June 2011 35 Chapter 10 Warranty Information Applied Biosystems warrants that chemicals and other consumable products will be free of defects in materials and workmanship when received by the buyer but not thereafter unless otherwise specified in documentation accompanying the product Applied Biosystems warrants that for a period of ninety 90 days from the date the warranty period begins the tapes diskettes or other media bearing the operating software of the product if any will be free of defects in materials and workmanship under normal use If there is a defect in the media covered by the above warranty and the media is returned to Applied Biosystems within the ninety 90 day warranty period Applied Biosystems will replace the defective media Applied Biosystems does not warrant that the operation of the instrument or its operating software will be uninterrupted or error free Warranty Period Effective Date Any applicable warranty period under these sections begins on the earlier of the date of installation or ninety 90 days from the date of shipment for hardware and software installed by Applied Biosystems personnel For all hardware and software installed by the buyer or a
31. decontamination procedure has been carried out before packing Refer also to Section 7 3 Decontamination procedure Ensure that the Certificate of Instrument Decontamination see Appendix A as well as the return authorization number RGA are sent with the instrument The Applied Biosystems technical service department will keep you up to date with the progress of service and provide you with any further details you might need for example on maintenance serviceability troubleshooting and replacement Applied Biosystems MagMAX Express User Manual Rev B June 2011 34 Chapter 10 Warranty Information 10 Warranty Information Instrument Warranty Information Computer Configuration Applied Biosystems supplies or recommends certain configurations of computer hardware software and peripherals for use with its instrumentation Applied Biosystems reserves the right to decline support for or impose extra charges for supporting nonstandard computer configurations or components that have not been supplied or recommended by Applied Biosystems Applied Biosystems also reserves the right to require that computer hardware and software be restored to the standard configuration prior to providing service or technical support For systems that have built in computers or processing units installing unauthorized hardware or software may void the Warranty or Service Plan Limited Product Warranty Limited Warranty Applied Biosystems warrants tha
32. e Perform planned maintenance procedures 2 1 3 Assumptions This guide assumes that your Applied Biosystems MagMAX Express has been installed by an Applied Biosystems technical representative This guide also assumes that you have a working knowledge of the Windows operating system 2 1 4 Text Conventions This guide uses the following conventions e Bold text indicates user action For example Type 0 then press Enter for each of the remaining fields e talic text indicates new or important words and is also used for emphasis For example Applied Biosystems MagMAX Express User Manual Rev B June 2011 8 Chapter 2 About the User Manual Before analyzing always prepare fresh matrix e A right arrow symbol gt separates successive commands you select from a drop down or shortcut menu For example Select File 2 Open gt Spot Set Right click the sample row then select View Filter gt View All Runs 2 1 5 User Attention Words Two user attention words appear in Applied Biosystems user documentation Each word implies a particular level of observation or action as described below Note Provides information that may be of interest or help but is not critical to the use of the product IMPORTANT Provides information that is necessary for proper instrument operation accurate chemistry kit use or safe use of a chemical Examples of the user attention words appear below Note The Calibrate function is also av
33. ential to transmit infectious diseases Follow all applicable local state provincial and or national regulations Wear appropriate protective equipment which includes but is not limited to protective eyewear face shield clothing lab coat and gloves All work should be conducted in properly equipped facilities using the appropriate safety equipment for example physical containment devices Individuals should be trained according to applicable regulatory and company institution requirements before working with potentially infectious materials Read and follow the applicable guidelines and or regulatory requirements in the following e U S Department of Health and Human Services guidelines published in Biosafety in Microbiological and Biomedical Laboratories stock no 017 040 00547 4 http bmbl od nih gov e Occupational Safety and Health Standards Bloodborne Pathogens 29 CFR81910 1030 http www access gpo gov nara cfr waisidx 01 29cfr1910a 01 html e Your company s institution s Biosafety Program protocols for working with handling potentially infectious materials Additional information about biohazard guidelines is available at http www cdc gov Applied Biosystems MagMAX Express User Manual Rev B June 2011 7 Chapter 2 About the User Manual 2 About the User Manual This user manual has been written for the actual end user for example laboratory technician and provides information on the MagMAX Express magneti
34. etimes but it will not affect the yield because the sample has been released from the particles Q8 Are the volumes of reagents in each well critical A8 We strongly recommend that you keep the given volumes within certain limits to avoid failure in performance of the chemical reactions and the processor Applied Biosystems MagMAX Express User Manual Rev B June 2011 33 Chapter 10 Warranty Information 9 4 Service request protocol If the MagMAX Express requires service contact your local Applied Biosystems representative or Applied Biosystems Do not under any circumstances send the instrument for service without any prior contact It is imperative to indicate the fault and nature of the required service This will ensure a faster return of the instrument to the customer Your local Applied Biosystems representative or distributor will take care of sending a complaint form that is the Warranty Claim Technical Sheet to the Applied Biosystems technical service department The Warranty Claim Technical Sheet contains a more detailed description of the fault symptom or condition Give all the necessary information to the distributor who will fill out and forward the Warranty Claim Technical Sheet to the Applied Biosystems technical service department Check Section 7 4 How to pack for service You will find instructions on how to proceed before shipping the instrument for service to Applied Biosystems Check that any necessary
35. his decontamination procedure enclose a signed and dated Certificate of Decontamination both inside the transport package and attached to the outside of the package 7 4 How to pack for service When you ship the instrument for service remember to Inform about the use of hazardous materials Decontaminate the instrument beforehand Install the transport lock Pack the instrument according to the enclosed packing instructions Use the original packaging to ensure that no damage will occur to the instrument during shipping Any damage will incur additional labor charges e Enclose a dated and signed Certificate of Instrument Decontamination see Appendix A both inside and attached to the outside of the package in which you return your instrument or other items e Enclose the return authorization number RGA given by the Applied Biosystems representative e Indicate the fault after you have been in touch with your local Applied Biosystems representative or Applied Biosystems See Section 8 1 for details on storage and transportation temperatures 7 5 Service contracts We recommend you maintain and service the instrument regularly every twelve months on a contract basis by the manufacturer s trained service engineers This will ensure that the product is properly maintained and gives trouble free service Contact the Applied Biosystems technical service department for more details 7 6 Disposal of the instrument e Warning Deco
36. imatiOn EE 8 39 P Packing ER a ia Aen aaa 17 27 34 IASTHUICTIONS a ad 17 27 MS a e Vida aa uae 17 POR iaa SE ege A A ee iu mitur Epica idi eg Sen 33 40 41 Plate EE 10 13 14 22 23 24 25 26 32 33 39 40 48 CM A a iaa 14 22 24 25 26 48 Plate Carrera e A A PORE D ga 14 22 24 25 26 48 Processing OEA A te eee any 14 Protocol cde EE a EE Eeer 10 21 33 34 41 48 R Releasing the transport lock vicio metti A UP LEER ee db eased EE 18 SI WEE EE 40 41 RRE le WEE A0 Applied Biosystems MagMAX Express User Manual Rev B June 2011 43 Chapter 13 Index S elle EE 10 11 14 18 23 26 28 33 40 48 SOVIET a sl 27 28 32 33 34 el Le 27 pack TEE 27 34 request lge ee EE 34 elle VE a das 24 DECHE EI HS ebe tidad deed gedet ratio mele red a e A det ees 8 28 29 general inu Ice iaa 28 PEO un Ee EE 28 MY tc 29 Status EE 20 21 23 Streptavidin coated magnetic particles oonoccccnnnnccconnnocancnnononnnonononn nn nora rra r narran arranca 40 edel LEET 6 T Table Of Figures MI 45 TIP COMAD e 10 13 14 15 16 22 23 24 28 32 33 40 48 Transport iconen 17 18 19 27 28 damages rose tio Lco ee o duit S mtd t eU i ELE RIT 17 o ee Emme 18 19 27 packages EG 17 27 lieri lel le Te ln e DEET 8 31 32 34 e Ile tt di ME 32 U Upa S t Rn I e m ee 17 User manua berast cio ec tiet eer edat nad dao nter decere eda a
37. ional reliability D O L1 O Design O L1 O O Ease of use D O O L1 Operational costs D O O O Customer support D O O O Further instrument system developments desired Further applications desired Where did you first learn about the product Would you like to receive information about other Applied Biosystems products Applied Biosystems MagMAX Express User Manual Rev B June 2011 49 18 Appendix D Addresses For the latest information on products and services visit our website at http www appliedbiosystems com Manufactured by Distributed by Thermo Fisher Scientific Oy Applied Biosystems Ratastie 2 P O Box 100 850 Lincoln Center Drive FI 01621 Vantaa Foster City CA Finland 94404 USA Tel 358 9 329 100 Fax 358 9 3291 0415 Tel 650 570 6667 www thermo com 800 874 9868 Applied Biosystems MagMAX Express User Manual Rev B June 2011 50
38. k that there is nothing preventing the track from moving left or right Check that there is nothing preventing the magnetic rods from moving up and down Ensure that the tip comb is fully in Check that there is nothing preventing the magnetic rods from moving up and down Contact authorized service Position the plate s correctly Push the microstrip s fully into the frame Position the plate s correctly Push the microstrip s fully into the frame Applied Biosystems MagMAX Express User Manual Rev B June 2011 32 Chapter 10 Warranty Information 9 3 Frequently asked questions FAQ about the MagMAX Express Q1 What does the MagMAX Express system do A1 Applied Biosystems now offers a complete MagMAX Express system the magnetic particle processor for the purification and processing of DNA RNA proteins and cells The processor handles particles automatically according to the preloaded purification protocols For more information on these applications contact Applied Biosystems Q2 Do need a PC to control the processor A2 Normally the protocol s is are preloaded by Applied Biosystems and no PC is needed for routine use KingFisher Software can be used to create new protocols or modify existing protocols if needed PC modified protocols are then downloaded into the processor for routine use Q3 Will the magnets get weaker If so how long can they be used Can they be replaced
39. ln c 42 14 Table Of e UE 45 15 Appendix A Certificate of Instrument Decontamination EE 46 16 Appendix B MagMAX Express Brief User s Guide een 48 17 Appendix C MagMAX Express Feedback Form c cccccssseececssseeeeeesseeeeeenseaeeeeenseeeeeenseaes 49 18 Appendix D Addresses 1o e rdg opo ep ip e dene SEENEN 50 Applied Biosystems MagMAX Express User Manual Rev B June 2011 3 Table of Contents Table of Contents 1 Safety Symbols and MarkingS ocoonnncccnononcccccnoncnccnnonnnononnonnnnnrnnnnnnnrnnnnnnrnnnnnnnr rr n nnne nenne nnne nnn nnns 6 1 1 Chemical Safety Guidelines ener nnne nennen nns 7 2 Bl0hazard WE ne RT H 2 About the User Manual rere tree tonne ge Teens aiii EE See 8 VADE Ee 8 2 1 1 Purposes or This e Le TEE 8 2 1 2 le EE 8 2 1 3 Ee e EE 8 2 1 4 A EE 8 2 15 User Attention WOrds mi een treo ii ri 9 2 1 6 Safety Alert WOrdS EE 9 2 2 How to Obtain More Information 9 2 2 1 Related DOCUMENTATION EEN 9 2 3 Howto Obtain Sup POM EE 9 3 Introduction to the MagMAX Evpnress ek NEEN 10 3 1 lach X 10 3 2 Principle of operation iei ore rr ero XGA e Ea er RO AE eX T e bud ES 10 3 3 Advantages of processing magnetic particles with the MagMAX Express 11 4 Functional Description ier reri eigener O a a 12 4 1 Instrument layout rte tere ha Pei Ee ER
40. luding CENELEC Common Modifications US and CA National differences Installation Category Overvoltage Category Il Pollution Degree 2 EN 61010 1 2001 Ed 2 EMC performance EN 50081 1 1992 Generic emission standard Residential commercial and light industry EN 50082 1 1997 Generic immunity standard Residential commercial and light industry EN 61326 1 1997 A1 1998 Product family standard A2 2001 A3 2003 Test standards Performance limits EN 55022 1998 Class B 150 kHz 1 GHz EN 61000 3 2 1995 A1 1998 Class A A2 1998 A13 1997 A14 2000 EN 61000 3 3 1995 EN 61000 4 2 1995 A1 1998 4 kV CD 8 kV AD Criteria B EN 61000 4 3 1996 A1 1998 3 V m 80 MHz 2 GHz Criteria A EN 61000 4 4 1995 1 kV Criteria B EN 61000 4 5 1995 2 kV line to ground 1 kV line to line Criteria B EN 61000 4 6 1996 3 Vims 150 kHz 80 MHz Criteria A EN 61000 4 11 1994 3096 10 ms Criteria B 60 100 ms Criteria C 100 20 ms Criteria B gt 9596 5 s Criteria C Applied Biosystems MagMAX Express User Manual Rev B June 2011 30 9 Troubleshooting Guide 9 1 Error messages and warnings Error message Warning Command error Cannot move track Head position error Head timeout Track position error Track timeout Magnets pos error Magnets timeout Serial number error Serialno already set Too high pos offset Invalid parameter Out of memory No acceleration tbl NV parameters lost Progmem full Ti
41. me already expired Chapter 10 Warranty Information Applied Biosystems MagMAX Express User Manual Rev B June 2011 31 9 2 Troubleshooting guide Chapter 10 Warranty Information Error Cause Action e Cannot move The track cannot be moved Press STOP If the fault cannot be remedied track because the head is not up then switch the power on and off e Head position The calculated head position does Check that there is nothing preventing the error not agree with the head home head from moving up and down sensor signal e Head timeout The head has not arrived at the home sensor within the allowed time e Track position The calculated track position does error not agree with the track home sensor signal e Track timeout The track has not arrived at the home sensor within the allowed time e Magnets position The calculated magnetic rod error position does not agree with the rod home sensor signal e Magnets timeout The magnetic rods have not arrived at the home sensor within the allowed time e Nonvolatile The nonvolatile parameters of the parameters lost instrument are lost e Clattering noise Plate s and or microstrip s is are not in place e Last release poor Plate s and or microstrip s is are not in place Check that there is nothing preventing the head from moving up and down Check that there is nothing preventing the track from moving left or right Chec
42. ntaminate the instrument prior to disposal See Section 7 3 and AN Appendix A Certificate of Instrument Decontamination on decontamination e WEEE symbol Do not dispose of this product as unsorted municipal waste Follow local municipal waste ordinances for proper disposal provisions to reduce the environmental impact of waste electrical and electronic equipment WEEE e European Union customers Call your local Applied Biosystems Customer Service office for equipment pick up and recycling See http www appliedbiosystems com for a list of customer service offices in the European Union e For more information contact your local Applied Biosystems representative Applied Biosystems MagMAX Express User Manual Rev B June 2011 27 Chapter 8 Technical Specifications 8 Technical Specifications 8 1 General specifications Technical specifications Overall dimensions Weight Operating conditions indoor use Transportation conditions Storage conditions Mains power supply Power consumption Heat dissipation Computer interface W x D x H 290 x 290 x 310 mm 11 4 x 11 4 x 12 2 in ca 10 kg 23 lbs 10 C 40 C RH 90 max 40 C 70 C packed in transport packaging 25 C 50 C packed in transport packaging 100 240 Vac 50 60 Hz nominal Automatic voltage detection 80 VA max 50 VA standby 273 BTU max Serial RS 232C port Baud rate 9600 Character format 1 start bit
43. nyone other than Applied Biosystems and for all other products the applicable warranty period begins the date the product is delivered to the buyer Warranty Claims Warranty claims must be made within the applicable warranty period or for chemicals or other consumable products within thirty 30 days after receipt by the buyer Warranty Exceptions The above warranties do not apply to defects resulting from misuse neglect or accident including without limitation operation with incompatible solvents or samples in the system operation outside of the environmental or use specifications or not in conformance with the instructions for the instrument system software or accessories improper or inadequate maintenance by the user installation of software or interfacing or use in combination with software or products not supplied or authorized by Applied Biosystems and modification or repair of the product not authorized by Applied Biosystems Warranty Limitations THE FOREGOING PROVISIONS SET FORTH APPLIED BIOSYSTEMS SOLE AND EXCLUSIVE REPRESENTATIONS WARRANTIES AND OBLIGATIONS WITH RESPECT TO ITS PRODUCTS AND APPLIED BIOSYSTEMS MAKES NO OTHER WARRANTY OF ANY KIND WHATSOEVER EXPRESSED OR IMPLIED INCLUDING WITHOUT LIMITATION WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE WHETHER ARISING FROM A STATUTE OR OTHERWISE IN LAW OR FROM A COURSE OF DEALING OR USAGE OF TRADE ALL OF WHICH ARE EXPRESSLY DISCLAIMED T
44. oe Era duco se TEuc Ege as SEE ego SEE 35 11 Ordering InformatiOn roce centia ee dicere dada 39 11 1 List Of ACCESO MES eade rta pe p deed nece erede Ee EEN 39 11 2 List of spare parts ede ci add dela dnb RE AER 39 11 3 List of recommended spare parts 39 12 Glossary and AbbreviatiONsS ooonoccnnnnncccnnoncccccnonnncononnnnncnnnnnnnnncnnn nn nn nnn nnne rn nan nr nnne nennen nnns 40 12 1 Keywords for web pages 40 12 2 literati alte Ate ime Lace Lebe i MEM Ee a iMd Aen LAM EAT dd 41 13 DY AO ee LH 42 14 ues lc cR 45 15 Appendix A Certificate of Instrument Decontamination EE 46 16 Appendix B MagMAX Express Brief User s Guide een 48 17 Appendix C MagMAX Express Feedback Form EE 49 18 Appendix E nudi pide rete etse Tr Ra Ren 50 Applied Biosystems MagMAX Express User Manual Rev B June 2011 5 Chapter 1 Safety Symbols and Markings 1 Safety Symbols and Markings These symbols are intended to draw your attention to essential information and alert you to the presence of hazards as indicated SAFETY SYMBOLS USED IN THE MagMAX EXPRESS Power ON Power OFF O Serial number ET Catalog number Date of manufacture BA Consult instructions for use WEEE symbol This product is required to comply with the European Union s Waste Electrical amp Electronic Equipment WEEE Directive 2002 96 EC WARNING MARKINGS USED IN THE DOCUMENTATION Warning risk of electric shock H Warning biohazard ri
45. owell can be larger than the volume of the next microwell and this is used for concentration purposes see Fig 4 7 below Y Transfer r v A y E gt El N j d v v j o rd Collection of Transfer into Release of Ready magnetic particles a low volume well magnetic particles suspension in a from a large volume low volume well well Fig 4 7 Concentration step during magnetic particle processing Applied Biosystems MagMAX Express User Manual Rev B June 2011 16 Chapter 6 Routine Operation 5 Installation 5 1 What to do upon delivery 5 1 1 How to unpack Move the unpacked instrument to its site of operation Unpack the MagMAX Express instrument and accessories carefully with the arrows on the transport package pointing upwards The following notes and instructions are sent with the instrument and are immediately available when you open the package packing instructions packing list MagMAX Express Feedback Form Warranty Certificate card MagMAX Express User Manual Note Do not touch or loosen any screws or parts other than those specially 1 designated in the instructions Doing so might cause misalignment and will void the instrument warranty Retain the original packaging for future transportation The packaging is designed to assure safe transport and minimize transit damage Use of alternative packaging materials may invalidate the warranty Also retain all instrument relate
46. ply an industry standard radioactivity decontaminant e g Radiacwash Rad Con or equivalent to the instrument and wipe surfaces as directed by the decontaminant manufacturer Survey the instrument with an appropriate radioactivity measuring instrument e g Geiger Counter or scintillation counter Satisfactory decontamination is defined as survey results at or below background level or in the US only for service work excluding transportation levels designated to be clean or safe as stated in the Customer s regulatory approved Site Radioactive Materials License Repeat steps a and b until satisfactory survey results are achieved and attach the results to this form 3 Hazardous Chemicals a Areas exposed to hazardous chemicals should be washed with an acceptable solvent such as ethyl alcohol or isopropyl alcohol b Rinse with detergent and water Mass Spectrometer Mass Spectrometers cannot be decontaminated at the customer s site Complete disassembly is required For PUMPS only a Drain any oils or other fluids and vent purge all gasses b Cleanthe outside of pump using the procedures described above c Seal all openings and securely package the pump to prevent damage Please note that Applied Biosystems cannot accept any instruments or parts that may be contaminated with viable biological agents harmful quantities of hazardous chemicals or radioactive materials Page 2 of 2 AB Part No 4343055 rev D
47. pparatus or reagents is conveyed expressly by implication or by estoppel TRADEMARKS Applera Applied Biosystems and AB Design are registered trademarks and MagMAX is a trademark of Applera Corporation or its subsidiaries in the U S and or certain other countries All other trademarks are the sole property of their respective owners Part Number N07848 Rev B June 2011 Applied Biosystems MagMAX Express User Manual Rev B June 2011 Contents Contents 1 Safety Symbols and MarkingS ocooonocccnononocccononcnccnonncnnncnnnnnnnnnnnnn cnn nnnnnnn nn nennen nn nnne nnne 6 2 About the User Manual aiii eie er eire poco iex HEC ec Eo tii 8 3 Introduction to the MagMAX Express ENEE 10 4 Functional Description iore tritt peer rre nere pne a Per ade npo an ic Dan eae Run noa ENK ard gps 12 5 EAE a a E A a ED T 17 6 Routine Operations sicsices sccsessscccecesccceeesscccuvceasceeeessocesedsnsecvevasceceveshocesvvssoceebavescecey shoceswysboceuvescees 21 7 Maintenant EE 25 8 Technical Specifications ciere eere enne ctia eene ax Eve ch dae ee anrea ducens 28 9 Troubleshooting Guide rere creer repe cre epe EL Rene cR FREE Re RE CHE e EXE DE Fee EE Re ER AERE 31 10 Warranty Information cn ciment eene eben ce 35 11 Ordering Information iio iii CE ARAN A cra a pe ENEE SNE 39 12 Glossary and Abbreviations cerise erecti tac licitadores 40 13
48. ramagnetic beads superparamagnetic microspheres template Applied Biosystems MagMAX Express User Manual Rev B June 2011 40 Chapter 12 Glossary and Abbreviations 12 2 Literature Aasheim H C Deggerdal A Smeland E B and Hornes E 1994 A simple subtraction method for the isolation of cell specific genes using magnetic mono disperse polymer particles Biotechniques 16 4 716 721 Coche T Dewez M and Beckers M C 1994 Generation of an unlimited supply of subtracted probe using magnetic beads and PCR Nucleic Acids Res 22 1322 1323 Cudjoe K S 1999 Immunomagnetic particle based techniques Overview In Eds Robinson R K Batt C A and Patel P D Encyclopaedia of Food Microbiology Academic Press London UK Pp 1088 1094 Hornes E and Korsnes L 1990 Magnetic DNA hybridisation properties of oligonucleotide probes attached to superparamagnetic beads and their use in the isolation of poly A mRNA from eukaryotic cells Genet Anal Tech Appl GATA 7 145 150 Jakobsen K S Breivold E and Hornes E 1990 Purification of mRNA directly from crude plant tissues in 15 minutes using oligo dT microspheres Nucleic Acids Res 18 3669 Jakobsen K S Haugen M Saeboe Larsen S Hollung K Espelund M and Hommes E 1994 Direct mRNA isolation using magnetic Oligo dT Beads A protocol for all types of cell cultures animal and plant tissues In Eds Uhl n M Hornes E an
49. sk Warning risk of personal injury to the operator or a safety hazard to the surrounding area OTHER MARKINGS USED IN THE DOCUMENTATION Note marks a hint important information that is useful in the optimum 1 operation of the system or an item of interest Applied Biosystems MagMAX Express User Manual Rev B June 2011 6 Chapter 1 Safety Symbols and Markings 1 1 Chemical Safety Guidelines To minimize the hazards of chemicals e Read and understand the Material Safety Data Sheets MSDSs provided by the chemical manufacturer before you store handle or work with any chemicals or hazardous materials e Minimize contact with chemicals Wear appropriate personal protective equipment when handling chemicals for example safety glasses gloves or protective clothing For additional safety guidelines consult the MSDS e Minimize the inhalation of chemicals Do not leave chemical containers open Use only with adequate ventilation for example fume hood For additional safety guidelines consult the MSDS e Check regularly for chemical leaks or spills If a leak or spill occurs follow the manufacturer s cleanup procedures as recommended in the MSDS e Comply with all local state provincial or national laws and regulations related to chemical storage handling and disposal 1 2 Biohazard Warning Biological samples such as tissues body fluids infectious agents and blood of humans and other animals have the pot
50. t SDS sodium dodecyl sulfate or soap solution If you have spilt infectious agents on the plate carrier disinfect with 70 alcohol or some other disinfectant see Section 7 3 Decontamination procedure Applied Biosystems MagMAX Express User Manual Rev B June 2011 24 Chapter 7 Maintenance 7 Waintenance 7 1 Regular and preventive maintenance For reliable daily operation keep the instrument free of dust and liquid spills Abrasive cleaning agents are not recommended because they are likely to damage the paint finish We recommend you clean the case of the instrument periodically to maintain its good appearance A soft cloth dampened in a warm mild detergent solution will be sufficient Clean the outside of the instrument and the plate carrier with clean low pressure compressed air or a cloth dampened with water or a mild detergent when necessary Although the MagMAX Express is constructed from high quality materials you must immediately wipe away spilt saline solutions solvents acids or alkaline solutions from outer surfaces to prevent damage be Note Painted surfaces can be cleaned with most laboratory detergents Dilute the cleaning agent as recommended by the manufacturer Do not expose painted surfaces to concentrated acids or alcohols for prolonged periods of time as damage may occur Clean the display areas with a mild laboratory detergent The keypad has a wipe clean surface Plastic covers and surfaces can
51. t all standard components of its Applied Biosystems MagMAX Express Magnetic Particle Processor will be free of defects in materials and workmanship for a period of one 1 year from the date the warranty period begins Applied Biosystems will repair or replace at its discretion all defective components during this warranty period After this warranty period repairs and replacement components may be purchased from Applied Biosystems at its published rates Applied Biosystems also provides service agreements for post warranty coverage Applied Biosystems reserves the right to use new repaired or refurbished instruments or components for warranty and post warranty service agreement replacements Repair or replacement of products or components that are under warranty does not extend the original warranty period Applied Biosystems warrants that all optional accessories supplied with its Applied Biosystems MagMAX Express Magnetic Particle Processor such as peripherals printers and special monitors will be free of defects in materials and workmanship for a period of ninety 90 days from the date the warranty begins Applied Biosystems will repair or replace at its discretion defective accessories during this warranty period After this warranty period Applied Biosystems will pass on to the buyer to the extent that it is permitted to do so the warranty of the original manufacturer for such accessories With the exception of consumable an
52. taining microstrips specially designed for the MagMAX Express ssssssssss 23 16 1 Mag MAX Express keypad i iiie bas tue t e RAT e EE M ra be s 48 Applied Biosystems MagMAX Express User Manual Rev B June 2011 45 15 Appendix A Certificate of Instrument Decontamination Applied Bibsystems Certificate of Instrument Decontamination This document must be completed in full and signed by the Customer a before returning an instrument or part hereinafter referred to as instrument to an Applied Biosystems Repair Center and b prior to any on site service to be performed by Applied Biosystems 1 Referto page 2 for special instructions concerning Mass Spectrometers 2 The form must be attached to the outside of transport packaging prior to returning the instrument to an Applied Biosystems Repair Center for repair maintenance trade in or loan European customers of Thermal Cyclers should fax the form to Applied Biosystems Customer Services and enclose a copy in the shipping container 3 The form must be given to the Applied Biosystems Engineer prior to any service of an instrument that has been located within a laboratory in which biological agents hazardous chemicals or radioactive materials have been used Please note that Applied Biosystems cannot accept any instruments that may be contaminated with viable biological agents harmful quantities of hazardous chemicals or radioactive materials AB Instrument Number
53. tegory overvoltage category defines the level of transient overvoltage which the instrument is designed to withstand safely lt depends on the nature of the electricity supply and its overvoltage protection means For example in CAT Il which is the category used for instruments in installations supplied from a supply comparable to public mains such as hospital and research laboratories and most industrial laboratories the expected transient overvoltage is 2500 V for a 230 V supply and 1500 V for a 120 V supply The pollution degree describes the amount of conductive pollution present in the operating environment Pollution degree 2 assumes that normally only nonconductive pollution such as dust occurs with the exception of occasional conductivity caused by condensation Both of these affect the dimensioning of the electrical insulation within the instrument Applied Biosystems MagMAX Express User Manual Rev B June 2011 29 Chapter 8 Technical Specifications 8 3 In conformity with the requirements The MagMAX Express bears the following markings Type 700 100 240 Vac 50 60 Hz 80 VA CE mark CSA monogram C Tick The MagMAX Express conforms to the following requirements 2006 95 EC Low Voltage Directive 2004 108 EC Electromagnetic Compatibility Directive EMC 2002 96 EC Waste of Electrical and Electronic Equipment Safety performance EN 61010 1 1993 A2 1995 IEC 61010 1 1990 A1 1992 A2 1995 inc
54. trument ona nano nar rr narra rra narrar rr 32 How to npack ie etuer Re A IA 17 How to use the keypad and display EN 21 How to work with a magnetic rod eeeseseseseessee nennen nnns nen nnn seni ian 15 Ineubatiori a ei pete criar eoo ELE coed e EENHEETEN EE 11 15 16 28 CEV e EE 8 17 18 27 29 SUMEN UU iia 12 TEN Use iia 10 Inverse magnetic particle processing oconconcncccnccnnnnnnnnnnnnnonccnnnnnnnnnnn nn nr nr 14 15 K Keypad and display 0 i 10 14 21 48 Ee 21 28 Keywords Tor WED pages ii rint rand n Zeg Ee E PEE i Pes nn arredi 40 Kite eiecit p reed te Toe ood trt vibe eee c E d cete ae be eee Ee Ote cra draco a eet 10 49 L Biet Lee Re Ee EE 21 LIE A de e ac E Bea ege state a e nde needs 41 M MagMAX Express 8 10 11 12 13 14 15 17 18 19 21 23 24 25 29 30 33 34 39 46 48 49 Magnetic particle processor issin ennan a nennen nnne nnn nnn nnn nnn nnne nnns 8 10 11 14 15 33 Magnetic particles 2er enini rabo onore a 10 11 14 15 16 33 40 collection Of ss eege edel dia 15 release of uniti tati ii nre os iem Dra an Pire ere Pare Eb ea cr STAT RR Drs Loa Y DA EAE E Fx SE YEA Ran Rd 15 WASH E EE NN Em 15 16 Mag ietic rod EE LLL 10 13 14 15 16 25 28 32 33 40 MAIS Soe ege ee Eege DEE eg ee ere 13 20 26 28 29 EI a Un BEE 25 27 48 TT Ee ER 8 25 27 34 MliCTOST EPEE A a a ad aia 10 14 23 28 32 40 O Operational check da 20 Ordering imfOr
55. xpress User Manual Rev B June 2011 9 Chapter 3 Introduction to the MagMAX Express 3 Introduction to the MagMAX Express 3 1 Intended use The MagMAX Express magnetic particle processor Fig 3 7 is intended for professional research use by trained personnel The instrument is intended for automated transfer and processing of magnetic particles in a microwell scale Use for self testing is excluded It is recommended that Good laboratory Practices GLP are followed to guarantee reliable analyses 3 2 Principle of operation The MagMAX Express magnetic particle processor Fig 3 7 is designed for the automated transfer and processing of magnetic particles in a microwell scale The principle of the MagMAX Express system patents pending is based on the use of magnetic rods covered with the disposable specially designed tip combs and microstrips These are provided with the reagent kit The instrument functions without any sucking or aspiration parts or devices Samples and reagents including magnetic particles are dispensed into the wells according to the corresponding kit instructions The use of microplate format allows the use of microwell scale volumes and existing liquid handling tools The steps of the protocol that are selected by the user via the keypad and display are preloaded in the on board software Fig 3 1 MagMAX Express magnetic particle processor Applied Biosystems MagMAX Express User Manual Rev
56. y is between 10 and 90 non condensing Leave sufficient space at least 10 cm at both sides and at the back of the unit to allow adequate air circulation The MagMAX Express does not produce operating noise at a level that would be harmful No sound level measurements are required after installation Note Do not operate the instrument in an environment where potentially damaging 1 liquids or gases are present 5 1 5 Things to avoid Do not smoke eat or drink while using the MagMAX Express Wash your hands thoroughly after handling test fluids Observe normal laboratory procedures for handling potentially dangerous samples Use proper protective clothing Use disposable gloves Be sure the working area is well ventilated Never spill fluids in or on the equipment pacemaker or metallic prostheses should not use this product A pacemaker or prostheses may be affected or damaged if it comes in very close contact with a strong magnetic field Warning This product contains strong permanent magnets People wearing a 5 1 6 Technical prerequisites Place the instrument on a normal laboratory bench The net weight of the unit is approx 10 kg 23 Ibs The instrument operates at voltages of 100 240 Vac and a frequency range of 50 60 Hz 5 2 Setups before you put the instrument into operation 5 2 1 How to release the transport lock Note THERE IS ONE TRANSPORT LOCK PRESENT Fig 5 2 A white tag with the red text

N07848_ver2.5 MagMAX Express User Manual

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