WIZ manual Vs 0.2beta _AJA
Contents
1. Click CONNECT to start communication check all the values against the test certificate received together the analyzer E SYSMEDIA WIZ CONTROL PANEL V 8 07 Main STATUS eral Direct comman d Graph if Result Edit Disk and fi mmands for active methods Single Wash Prime Dilution ratio Dilution factor Sample and reference visualization Temperature_1 setting EEE CMETHE C WET METH1 METH sees Sut THANT METH2 METH2 VISET START DILUTION C METH3 METH4 METH3 METH3 Ref 0 Ref 1 Ea 4 TNE START CALIBRATIONS 3 METH4 METHS Se eI LEDS ete Read dilution ratio Read dilution fact START READ MONITOR and ATC r Read temp values Send POX 000 MONITOR PARAMETERS SET READ DATE AND TIME ITE iT gt READ ae ae Lo ae READ water LED current setting gt READ DATE TIME FROM INSTRUMENT LED N i DAC VAL a UPDATE WITH LOCAL PC DATE TIME gt READ Page 34 of 37 WIZ manual Vs 0 2beta AJA 6 Switch to the Operativity folder 7 Select Method then start a first Wash 8 Repeat Start Wash to fill up the WIZ hydraulics with E SYSMEDIA WIZ CONTROL PANEL V 8 07 UKAS QUALITY MANAGEMENT ISO 9001 2008 273 Certificate number AJAEU 10 12116 9 Switch to the graph folder and click on Direct commands Graph and O D display E SYSMEDI2. Page 25 of 37 WIZ manual Vs 0 2beta AJA ISO 9001 2008 273 Certificate number AJAEU 10 12116 Reagent canister is now ready to be fitted on the WIZ 4 24 Reagents Canister mounting Page 26 of 37 WIZ manual Vs 0 2beta AJA ISO 9001 2008 273 Certificate number AJAEU 10 12116 1 Insert the reagent canister on the proper holder on the top of the WIZ taking care to match the driving pin located on the left side Push gently until the connector fit completely i f ATER a B ej ANALY Page 27 of 37 WIZ manual Vs 0 2beta AJA ISO 9001 2008 273 Certificate number AJAEU 10 12116 2 Fix the cylinder by screwing firmly the top screw then open the reagent connector rotating CCW the cylinder body to allow reagents to flow inside the probe 4 2 5 External canister for DI water and toxic waste Di water and toxic waste are contained in 2 external bags 600 mL each External bags are inserted in an external canister the connected to the WIZ from top connectors Page 28 of 37 WIZ manual Vs 0 2beta AJA ISO 9001 2008 273 Certificate number AJAEU 10 12116 List of operations a b c d e f Fit both bags inside the canister Mount the connection tubes Position upside down the Canister and fill the DI water bag with a 50mL syringe Pinch the DI water bag tube Mount the canister on the WIZ Connect both bags to respective positions Page 29 of 37 WIZ manual Vs 0
3. e multi beam fiber optic colorimeter with Power supply 12 Vdc provided through the main silicon detector connecting cable Fluorimetric excitation 370 nm emission Power absorption 3W in stand by 8 W mean 420 470 nm 1 cm during analysis Type of analysis sequential batch Weight in air 8 Kg Analysis interval programmable Dimensions 140 mm _ diameter x 520mm Measuring time 30 minutes for a full 4 height analytical unit 70mm diameter x 200mm parameters cycle height reagents canister Number of parameters up to 4 Environmental requirements WIZ should be ae operated with the ambient temperature ranging M h 1 en m SA CEDI n from 4 C to 40 C A temperature near 0 C may Body material PVC freezes the reagents and the calibrant a Operating temperature 4 40 C temperature over 30 C will reduce the reagents Hydraulic connections std 3 2x1 6 mm stability and life Waste directly discharged in water toxic Standard package includes wastes collected in a separate bag located analytical and electronic units Reagent expiring min 4 6 weeks depending 5 m submergible cable on the method WIZ panel software start up kit Autonomy up to one 45 days depending on analysis frequency Optional accessories Additional R t ist Hardware industrial PC onal Reagent canister 104 CPU externally programmable by serial communication port Data output RS232 serial port Page 6 of 37 WIZ manual
4. ANALYTICAL TECHNOLOGIES Version Prepared Verified Controlled Approved Issued PM TM QM MD osx S a S Production Sales and Service Via Paduni 2A 03012 Anagni FR Tel 39 0775 776058 Fax 39 0775 772204 e mail anagni systea it Web site http www systea it ISO 9001 2008 273 Certificate number AJAEU 10 12116 SYSTEA SpA WIZ Probe USER MANUAL Ver 1 0beta SUMMARY l INI RODUCTION ener E A ARAA 4 1 1 ANECA ET E paa E S cataneennsteetsueamaednadiasens sactheanastewtemaastdns 5 EEE e Ea a E E E EE rea E E N E E E A T E 5 1 2 W o a A e E E N O 6 1 3 FlowChart Standard Nutrients version ccccceccccesecccseccceecsceessceeseeeueceeseceeeecseeuss 7 Z ANALYTICAL CYCLES DESCRIPTION 0 cccsccccsseesssesessssesseeseseseesesseseseenssenes 8 2l LOOP ai calc CTO e E E 8 22 SAO e E E E T E cpeasadgaassSuduamvasaneenns 8 2 3 PRIN ANY S15 oo vais a te ete ecle E E aie ete cclsnaesa ee hecien assent a eet ensanee da E E 8 Zee WRC AC CIE TM CCT aca ae secre A nations averse E A A E ET 8 2o M a E E E E E E aeetennctdate 8 29 ReACtiOMmanG OD reddine aicese E 9 LA E OS e A E 9 DWV AS ate E E E E A S A O E E teers eececcteeceee 9 2 4 CADORE CIC oae E E E E AE RA 9 3 YY TZ OW TeV EW serere E A EN NS 10 l External OY CP VC W seonilesseidhe renea Ea eaa 10 3 2 Analytical and electronic internal ViCW ccccccccecccccceeeeeeesssseseseeeecceeeeeeeeeeeeeaaaaas 11 32A AMARC Al nemal VIEW sss
5. 2beta AJA ISO 9001 2008 273 Certificate number AJAEU 10 12116 Com o x po gt Las 4 gt y h A he a at AWT gt a Bare r et aes p A T a ET a a in s Bags canister assembly front view Page 30 of 37 WIZ manual Vs 0 2beta AJA ISO 9001 2008 273 Certificate number AJAEU 10 12116 Mount the canister on the WIZ Connect both bags to WIZ Page 31 of 37 WIZ manual Vs 0 2beta AJA ISO 9001 2008 273 Certificate number AJAEU 10 12116 4 3 Power and sample connection When mounting is completed final assy looks as follows Page 32 of 37 WIZ manual Vs 0 2beta AJA ISO 9001 2008 273 Certificate number AJAEU 10 12116 Connect the power cable to the power connector located on the top of WIZ wait for booting about 45 60 seconds then connect the sample line Note that power cable includes also the serial connections On the power side of the WIZ cable is available as standard an DB9 RS232 connector WIZ now is ready for start up as described in following chapters 5 Operation WSIZ can be controlled and operated trough an external software the WIZ Control Panel a The WIZ Control Panel Running on PC under Windows OS dialogues with the probe using the RS 232 serial port are available from the external electric connector The Control Panel allows to edit modify the run settings display graph the real time Optical Density measured by the colorimeter
6. 6 Complete mounting by screwing gently the fixing screw on top of the frame Page 20 of 37 WIZ manual Vs 0 2beta AJA ISO 9001 2008 273 Certificate number AJAEU 10 12116 c Open the reagent connector D A Te x 5 pak gt TA s q y Di vue i E B 7 7 s Che wy Y s i p s T i a CA TAN i l a 5 7 i y Ga A ery x a r wa 4 k a ct a L lt d Remove the stopper form the reagent inlet to be filled Page 21 of 37 WIZ manual Vs 0 2beta AJA ISO 9001 2008 273 Certificate number AJAEU 10 12116 e Open the Roll On stopper f Connect the filling syringes as for following Steps DI water syringe wash the bag al least twice by injecting 40 mL of Di water then aspirate back the wash water discharge the water after each wash and refill the syringe with fresh DI water before next wash Remove the DI water syringe Reagent syringe filled with 25mL of reagent prime the bag by injecting 25mL of reagent aspirate back the reagent and discharge Reagent syringe filled with 50mL of reagent inject 40 45mL of reagent in the bag Close the Roll On stopper Remove the syringe Switch to the next bag Page 22 of 37 WIZ manual Vs 0 2beta AJA 7 ISO 9001 2008 273 Certificate number AJAEU 10 12116 After all bags are filled close the reagent connector then remove the bag frame Wash the filling tool by connecting a waste syringe the line and open the r
7. display the sample measures stored by the unit edit modify the WIZ monitor settings download the memory buffer with measured data in text format WIZ software allows to manage all the operations normally required by the user during measurements Page 33 of 37 WIZ manual Vs 0 2beta AJA 6 Start up 5 All 1 Turn On the WIZ 2 Turn On the PC after booting run the WIZ Panel 3 On the Main folder first select the COM used by the PC then click on Connect like 4 The bottom message box will prompt the user when connection will be established fields Working Calibrants Calibrants OD etc will be updated with the values stored inside the WIZ Please SYSMEDIA WIZ CONTROL PANEL Y 8 07 ISO 9001 2008 ALITY 010 MANAGEMENT 273 Certificate number AJAEU 10 12116 After Setup WIZ can be connected to a 12 V power supply and a PC with the WIZ panel SW installed PC has to be connected trough a serial port to the WIZ All following steps are given only for Method 1 repeat all steps for all methods included in the WIZ delivery Note following software images may be not updated to the last software revision use them only as general reference for more detailed and updated information please use the document WIZ Panel SW manual Method 1 Method aj Method 2 Method 2 2 Metho Method 3 Method 3 Method 4 Method 4
8. properly sealed and specifically tested under water pressure Upon receipt check for any visual and or external damages taking care to check the conditions of all electrical and hydraulics connections WIZ Reagent Canister is delivered fully assembled To setup WIZ a J Fill all bags Fill all reagents bag or only the reagent bags for the active methods Fill the Calibrant bag Fill the water bag Connect the Reagent canister on the WIZ and open the reagents connector Connect the water line and the toxic waste line Connect the sample line Power on the WIZ 12 V DC Wait for booting Connect laptop to the serial port Run WIZ control Panel See the software manual for details For first setup run twice a Reagent Prime after reagent changeover one prime is enough Run a Wash Run Reagent blank check stored OD Blank against the OD Blank reported on the factory test Run Calibration check stored OD cal against the OD Blank reported on the factory test WIZ is now ready for deployment or any other use Page 13 of 37 WIZ manual Vs 0 2beta AJA aman ISO 9001 2008 273 Certificate number AJAEU 10 12116 4 2 Reagents Canister Set up 4 2 1 Reagents Canister description Extracts the reagent bags frame from the PVC cylinder have access to the bags Each bag is numbered has two ports one is used to connect the bag to the connector trough a Teflon tube and one is closed and is used to refill the bag trough a syringe B
9. A WIZ CONTROL PANEL V 8 07 Commands for active methods Single Wash Prime Di STATUS 18 Go back to the main folder 19 Click on Disconnect 20 Click on Connect ilution ratio 1 r Dilution factor r Sample and reference visualization Temperature_1 setting j METH1 METH1 Sample Q Sample 1 START ANALYSIS PEN Maa EH i MANT METH2 METH2 RVISET START DILUTION C METH3 METH4 METH3 METH3 Ref 0 Ref 1 See 9 mme START CALIBRATIONS i METH4 METH4 Read dilution ratio Read dilution fact START READ r MONITOR and ATC r Read temp values Send POX 000 r MONITOR PARSMETERS SET READ DATE AND TIME Lec READ LED current setting gt READ DATE TIME FROM INSTRUMENT LED N C f DAC VAL UPDATE WITH LOCAL PC DATE TIME gt READ 21 The bottom message box will prompt the user when connection will be established 22 All fields Working Calibrants Full scale Calibrants OD Reagent Blank OD will be updated with the values stored inside the Start Graph to check the measured OD check the value against the Water OD value reported on the test report received with the analyzer NOTE When the real time display is active all other commands are ignored to send any other command it is necessary first to click on STOP Graph 10 Repeat from 8 if graphs and consequently OD value is not stable meaning that the hydraulics is not completely filled wit
10. Vs 0 2beta AJA ALITY QU MA AGEMENT ISO 9001 2008 273 Certificate number AJAEU 10 12116 1 3 FlowChart Standard Nutrients version WIZ FlowChart NH3 OPA NO3 UV PO4 amp NO2 Mixer gt N LT a 1 VK RED F C 20 mm 128 525 nm 129 A 880 nm Manifold valve position front view To detector ef 1s 125 Oo FLUORIMETER F C 10 mm O e 189 370 nm ON T O O 190 370 nm OFF 171 V10 af 10 e Waste WT Toxic Waste WN Normal Waste 52 56 58 Pump direct fast 175 Pump reverse 83 Pump direct slow Vi B V2 A V3 A v4 O V5 A V6 A Dilution codes for V10 and V11 V10 177 X Y where X on sample Y off buffer B V11 178 X Y where X on sample Y off diluent H Normally sample time X is 1 second Reset V10 V11 dilution 179 O C1 R1 PO4 Color O C2 R2 PO4 Ascorbic O C3 R3 NO2 NO3 SAA O C4 R2 NO2 amp R2 NO2 NO3 NED O C5R1I NO2 amp R3 NO2 NO3 DTPA O C6 NH3 OPA O C7CAL V1l VK6 O O ap Q Y LG 108 133 1 b C10 H S Wash DRS of 37 SAMPLE Solution WIZ manual Vs 0 2beta AJA ISO 9001 2008 273 Certificate number AJAEU 10 12116 2 ANALYTICAL CYCLES DESCRIPTION 2 1 Loop Flow Reactor WIZ analytical reactor includes all necessary devices mechanical hydraulics optics sequentially connected to form a ring defined as micro Loop Flow Reactor in short wLFR The uLFR hydraulic diagram is shown in the previ
11. aaa e Ce eS perme e container staat was a l a aa 2 Start Analysis START BLANKS o so MONITOR and RTC r Read temp values Send POX 000 r MONITOR PARAMETERS SET READ DATE AND TIME See f ompmac ___ Date Eoo _ gt Maintenance m Ests UMAC Me O o Ss READ arameters m Activate MAC Monitor LED curren t setting lt START gt gt READ DATE TIME FROM INSTRUMENT LED N C i Sampling frequency Minutes Recalibratiori pes 1 UPDATE WITH LOCAL PC DATE TIME gt BEAD ave frol osition a D STATUS 6 2 Start Monitor 1 Switch to Operativity folder 2 Click on Read Monitor Parameters from uMac to update the Sampling Frequency Recalibration frequency and Save from position fields with the data stored in the NPA PRO memory Page 36 of 37 WIZ manual Vs 0 2beta AJA E SYSMEDIA WIZ CONTROL PANEL Y 8 07 ALITY 010 MANAGEMENT 273 Certificate number AJAEU 10 12116 ISO 9001 2008 a 3 Check if the data downloaded form the Note Direct comman d Graph il Result Edit if Disk and fil 7 DERE NS fer active metheds e Dilution ratio Dilution factor Sample and reference visualization Temperature_1 sett WIZ Memory are matching the actual arses cm cums Fe De C e needing if not change the values and click START DILUTION C METH3 C METH4 METH3 E m mE i E a E on U
12. ags numbering match the reagents number as reported in the hydraulic layout A Reagent canister guard B Reagent bags and frame C Reagent Canister cap D Fixing screw E Reagent Connector F Open Close key Bottom Details Page 14 of 37 WIZ manual Vs 0 2beta AJA ISO 9001 2008 273 Certificate number AJAEU 10 12116 4 2 2 Reagent and Calibrant bags filling To fill the regents amp calibrant bags use the filling tool delivered with the WIZ as for following procedure Extract the reagent frame from the canister guard take care that when delivered the frame has a safe lock on the bottom that has to be removed before frame extraction Mount the frame on the filling tool Open the reagent connector Remove the cap from the line inlet Open the Roll On stopper of the line to be filled Connect a 50mL syringe with DI filled with Di water Wash the bag with DI water repeat twice Connect a 50mL syringe filled with 25mL of reagent Prime the bag with all 25mL then empty the bag and waste the regent used for priming Refill the syringe with 50mL of reagent then fill the bag with 40 45mL Close the Roll On stopper amp remove the syringe Repeat for all lines Close the reagent connector Remove the frame form the filling tool Insert the frame in the canister guard taking care do not pinch any tube a Extract Ragents frame To extract reagent frame take care to remove the safety lock on the b
13. diluted calibrant the calibration cycle runs same steps as described in 2 Analysis Page 9 of 37 WIZ manual Vs 0 2beta AJA AWA STEA 3 WIZ OVERVIEW ISO 9001 2008 273 Certificate number AJAEU 10 12116 3 1 External overview WIZ consists of PVC canisters 1 WIZ Probe including a hydraulic manifold b electronic boards c Detectors d Etc 2 Reagent Canister including Reagent and calibrant bags Water bags Toxic Waste bag Multireagent connector Reagent Canister Wiz top view Page 10 of 37 WIZ manual Vs 0 2beta AJA 3 2 Analytical and electronic internal view 3 2 1 Analytical internal view Front side A Manifold amp valves B Pump Motor C Pump Head D Colorimeter Flowcell assy E Fluorimeter Flowcell assy F Heating bath G UV Digestor H UV Digestor ballast I Electronic Enclosure Page 11 of 37 WIZ manual Vs 0 2beta AJA ISO 9001 2008 273 Certificate number AJAEU 10 12116 Back Side QUALITY MANAGEMENT ISO 9001 2008 273 Certificate number AJAEU 10 12116 3 2 2 Electronic compartment internal view E TESA i titithe T TEELT Be EEN JE ret A ae eS a od eal AN ae i CPU and ne MPIO bbard Page 12 of 37 WIZ manual Vs 0 2beta AJA ISO 9001 2008 273 Certificate number AJAEU 10 12116 4 SETUP 4 1 General check WIZ probe is delivered fully tested with the Electronic and Analytical module
14. for in situ long term measurements based on a new analytical technology named uLFA micro Loop Flow Analysis WIZ allow to analyse sequentially up to four nutrients can be installed on a buoy or any other floating support and can be submerged down to 8 m depth It is also designed for hydrology applications where the probe can be used as a portable field analyzer for on site measurements The basic WIZ configuration allows to measure NH3 NOQ2 NQO3 NO2 PO in water or seawater other parameters are available on request Possible configurations are WIZ 1 single parameter WIZ 2 two parameters WIZ 3 three parameters WIZ 4 four parameters Basic Wiz includes Analytical reactor with a miniaturised valves manifold the pump and the flow cell connected to the light sources and the detector through optical fibers Electronic compartment with power supply board PC 104 CPU board preamlipfier board and detector etc WIZ is delivered as standard with a Reagents Canister containing the a series of bags to store reagents calibrant water and wastes The Reagent Canister is connected to WIZ analytical reactor trough a 10 positions connector allowing fast connection disconnection of the whole canister Patent pending Page 4 of 37 WIZ manual Vs 0 2beta AJA UALITY Q MANAGEMENT ISO 9001 2008 273 Certificate number AJAEU 10 12116 1 1 Analytical cycle WIZ can run sequentially one or more anal
15. h DI water 11 Switch back to the Operativity folder 12 Select Method 1 Start a Prime to fill the Method 1 reagent lines 13 Repeat for all methods 14 Start blanks 15 Switch to Graph folder to follow the OD during the reaction process 16 Start calibrations 17 Switch to Graph folder to follow the OD during the reaction process Page 35 of 37 WIZ manual Vs 0 2beta AJA Ww UKAS QUALITY MANAGEMENT ISO 9001 2008 273 Certificate number AJAEU 10 12116 WIZ Take note of the new Calibrant x OD and Reagent Blank OD and check w Ew them against the test certificate received i to ether the anal Zer Switch remote COM port a Telephone number Method 1 Method 3 Method 2 Method 3 C etree prO r Method 3 Method 3 Method 3 Method 3 C Pees Method 4 si Method 4 sd Pereda Method n me me a s ar r Click CONNECT to start communication ATER ANALY ER A SYSMEDIA WIZ now is ready for one of following 6 1 Analyse a spot sample E SYSMEDIA WIZ CONTROL PANEL V 8 07 1 Switch to Operativity folder Commands for active methods Single Wash Prime r Ciluti id r g isn ratio ution factor r Sample and referer C METH C METH METH1 METH1 Sample p THANT METH2 METH2 swam ionic 2 Insert the sample line in the sample START ANALYSIS l l XVISET t 1
16. he three way injection valves and the mixer allow a fast and effective mixing Page 8 of 37 WIZ manual Vs 0 2beta AJA ISO 9001 2008 273 Certificate number AJAEU 10 12116 2 3 3 Reaction and OD reading The reaction takes place in the colorimeter flow cell the software allows the graphic monitoring of the reaction development up to the end point If method requires heating to reach the colour development i e NH3 the sample is heated in a proper device then moved inside the detector As soon the reaction reaches the end point O D is measured and stored 2 3 4 Calculations Reagent Blank OD stored and used to calculate the calibration factor Calibrant OD stored and used to calculate the calibration factor OD Start Sample Blank OD OD End Sample final OD OD Start Sample concentration OD End Reagent Blank OD x Calibration factor 2 3 5 Wash Valves S L on SAMPLE all other valves off while pump P runs Di water form V11 enter the loop and comes out form V8 As soon the washing time expire pump P stops and S L are switched off the loop remains filled with DI water ready for a new run 2 4 Calibration cycle Allows the instrument recalibration and QC check using a concentrated calibrant solution that when diluted match to the calibration span Calibration solution is pumped inside the loop while is diluted by modulating the calibrant valve VK5 between calibrant ad Di water After filling the loop with the
17. oll on stopper while the syringe is aspirating form the tube to remove the reagents inside the tube After all lines are empty wash one by one with DI water syringe if DI water flow out from the reagent connector dry with paper 4 2 3 Reagents canister assembly After all bags are filled reagents canister may be mounted and fitted on the WIZ 1 Identify inside the canister guard the pin A and the bottom squared holder B Page 23 of 37 WIZ manual Vs 0 2beta AJA ISO 9001 2008 273 Certificate number AJAEU 10 12116 2 Identify on the bags frame the 3 Indentify on the bottom of the frame the squared white two cuts that have to be matched plastic part that allow to open close the reagent flow with the pin A d Insert the bags frame inside the canister taking care to match the reference points as showed in and 2 If 1 and 2 match correctly the bottom squared white plastic showed on 3 match the square holder B of the canister guard During insertion may be necessary rotate slightly the frame to fit perfectly Take care to avoid tube and bags pinch during assembly Page 24 of 37 WIZ manual Vs 0 2beta AJA QUALITY MANAGEMENT ISO 9001 2008 273 Certificate number AJAEU 10 12116 Correct mounting allow the reagent connector to come out from the canister bottom for few millimetres if not mounting is not correct and the squared white plastic did not match the holder on the bottom of the canister
18. ottom of the Reagent Canister Then Page 15 of 37 WIZ manual Vs 0 2beta AJA ISO 9001 2008 273 Certificate number AJAEU 10 12116 a l Remove the canister cap a 2 Extract the Reagent bags frame by pulling up the Fixing screw Help extraction by pushing gently from the bottom the reagent connector out coming form the canister guard Bags frame extracted Page 16 of 37 WIZ manual Vs 0 2beta AJA ISO 9001 2008 273 Certificate number AJAEU 10 12116 Bottom part to push gently Pull up the fixing screw Page 17 of 37 WIZ manual Vs 0 2beta AJA AWA OSIEA b Mount the frame on the filling tool b 1 Filling tool descripion ISO 9001 2008 273 Certificate number AJAEU 10 12116 A Reagent connector B Reagent connector arm close open C Roll On stoppers D Reagent Inlets E Filling Syringe use dedicated syringe for each reagent b 2 1 Be sure all Roll on stopper are closed b 2 3 Identify the male pin on the filling tool and female pin on the bottom of the reagent frame Page 18 of 37 WIZ manual Vs 0 2beta AJA ISO 9001 2008 273 Certificate number AJAEU 10 12116 b 2 4 Insert the reagent connector arm that allow to open close the reagent connector for filling Page 19 of 37 WIZ manual Vs 0 2beta AJA ISO 9001 2008 273 Certificate number AJAEU 10 12116 b 2 5 Mount the bags frame taking care to match the male and female pins b 2
19. ous figure shown components allows to run up to four chemistries with the possibility to select two different detectors colorimetric and fluorimetric and on the colorimetric detector 2 different wavelengths Valves S L V8 and V7 allows to open the loop to allow pumping of sample standard or blank solution SAMPLE position or to keep the loop closed LOOP position to allows mixing of reaction product The reagents and the calibrant are introduced in the loop by the pump an effective and fast mixing is ensured by a mixer mounted just after the pump 2 2 Sampling With valves S L open the pump P is activated the sample is pumped trough VK6 V11 VK5 and V7 inside the loop passing through the mixer and the colorimetric flow cell then it flows out to the waste through V8 The loop including the flow cell is now filled with sample the system measure and store the Sample Blank value that will be subtracted from the final OD of the reaction product 2 3 Analysis Following operations are valid for Sample Reagent Blank and Calibration 2 3 1 Reagent injection Valves S L are in SAMPLE then reagent valve connected to the reagent bag is switched on while the pump P is activated for the programmed time to inject an defined quantity of reagents inside the loop 2 3 2 Mixing Valves S L are positioned in LOOP position the pump P is activated to allow a fast mixing of sample and the reagents The particular internal layout of t
20. pdate to store the new settings inside START BLaNks ead diution ratio Read diution fact stant READ Send POX 000 epi lO SET READ DATE AND TIME ia p gt e100 4 Click on Start to start monitoring oe READ gt READ DATE TIME FROM INSTRUMENT pacvat STATUS UPDATE WITH LOCAL PC DATE TIME gt READ 1 Before start monitoring WIZ will wait for a time equal to the Sampling frequency to allow the deploy of the probe in water In case of Laboratory tests where the user may need the first results immediately switch back to the Operativity folder and click on Start Analysis WIZ will start immediately then will wait for the next analysis until the Sampling frequency time expire 2 After clicking Start the WIZ timer counting the elapsed time on the Sampling frequency will remain active also if the probe will be shutted down This is to allow WIZ deploy without PC connection Page 37 of 37 WIZ manual Vs 0 2beta AJA
21. rinin Mewetasaeesanssciistecadenieneden 11 32 2 Electronic compartment internal VIEW cccccccccccccccececeeeeeessssssseeeececceeeeeseeeaaaqags 12 4 ETOP oe circ mecitese E E E E E 13 4 1 SINS E E E E E A N EN 13 4 2 Rean mts Canister Set Dee 14 42 1 Reagents Canister AeSCription ccccccccccccccccccceeeeeeessseseeeeeeececeeeeceeeeeeseaaeaeseeeeeees 14 4 2 2 Reagent and Calibrant bags filling snennonessssssssseeeeeerersssssssssssssssseseereeresssssssssss 15 423 ARCAGCEIIS CAMISTCR assembly ienesis E aE a a 23 Page 2 of 37 WIZ manual Vs 0 2beta AJA NWA SIEA ISO 9001 2008 273 Certificate number AJAEU 10 12116 424A Reagents Canister MOUNDE sierici E nE EE EEEE E 26 4 2 5 External canister for DI water and toxic Waste cccccesseeeecceceeeeeeeeeeaaaeeeeeeseeeees 28 4 3 Power and Sample connection ccccsssssseececceceeeeeeeeceaaeaeesesseseeseeeeeeeeeeeeeeeeeeaaaaags 32 4 3 1 Power amp Sample line connection eeesssssssssssssssseeeeteeeressssssssssssssseeereeereessssssssss 33 OPa O E E EA 33 l PA OUD e e E E E sataaces 34 6 1 Analyse aopo Sap e A scutenntaeetuenee 36 6 2 SEMONO aaaea ENE N AN E E N 36 Page 3 of 37 WIZ manual Vs 0 2beta AJA T O igi e e AAAA AY ORANI i s ly 7 UKAS QUALITY MANAGEMENT ISO 9001 2008 273 Certificate number AJAEU 10 12116 1 INTRODUCTION WIZ Probe is an innovative automatic chemical analyser
22. ytical cycles chemistries the necessary steps to complete an analytical cycle are Sample introduction in the analytical reactor Sample blank measure and storage OD Blank Reagents injection with proper sequence and timing Sample and reagents mixing Heating if needed Reaction time Reaction Optical Density measure OD End and storage a oe ae a E Calculation of the sample concentration using the stored calibration factor The analytical cycle during monitoring is repeated for the next active method up to four times 1 1 Calibration cycle The mixed concentrated calibrant is a solution including all the analytes to be detected It is placed inside the Reagent Canister in a 50mL bag and is automatically diluted during calibration cycles A calibration cycle replicates exactly the Analysis cycle steps introducing in the analytical reactor a diluted calibration solution instead of the sample Calibration cycle is repeated for each method and the calibration factor is automatically calculated and stored Page 5 of 37 WIZ manual Vs 0 2beta AJA ISO 9001 2008 273 Certificate number AJAEU 10 12116 1 2 WIZ technical data Technical data Programming functions provided by the external Measured parameters 4 WIZ control panel software Standard NH3 NO3 NO2 NO2 PO External connections 6 pole submergible cable other methods available on request for serial data communication and 12 Vdc power Detectors supply
Download Pdf Manuals
Related Search