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Electrophoresis -EGel and Transilluminators
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1. Conon one pane NN ecc T R Electrophoresis E Gel System E Preparation 1 Pias the Version into a electrical sedente adapto 1 plug The base will perform a rie selt et With no gel in the boc no light tell be illuminated an the baoe ae sh tive Lo Insert the E Gel gel with the comb in place into the right side hele ides pace here shoul bean adie cic See 3 A steady red light will illuminate when the E Gel gel is correctly inserted E NI Top Bottom 259 CPLS Morin 2018 Page 38 Electrophoresis E Gel System Basic Operations Basic Operation Interrupt Operation of the PowerBase Version 4 is contralled by two buttons top of the base The left button is for a double comb and the right button is for a Fall single comb run oe the label on the unit To select different electrophoresis runs for the PowerBace do one of the following Pressand release the button OR Press and hold the button Each electrophoresis run is indicated by the light next to the button Please read the instructions on the next page for more details Se ql Electrophoresis E Gel System Pre run and Running Gel You mst presen get for unutes th
2. Electrophoresis ElChrom Power Unit Controls CPLSMorson gota Maia Ferrovie Saree STEP START Sip STARTIed Led avenge node Lad Tius xece Led Voltage ices cras Cuin timer swatch CLEAR rich Trading sede Tensen at ospiti Vine Dissley ape dor ia nins mur Bega STOP mode STAR Turc geom ad or antec node vices set poirt Dexsesekeciemo oent eet point tue et nade Potties CORE rel 3 of reviens ning Kid rezati Display ouput set vus Page pens atu m e imd ee ende EL 2 STANT c ith Te START LED Twine ard de Late operan on fe sitar ot mure one tt Te be wade Lege C VOLTE MIAME wl ict nding te preme consi Sori geo ease cut fr Hose rion le tang cer These accra resort Sarge in eras ons hector ri dus VO cop be sip A dolay to cupit aes ae lrg as READ NG cw hoo ma FEADING me Las ft ho ot aes os
3. release either button to stop the beeping The light wel tumm to a steady Jed ight Remove gal trom Me now sean the Pat pel tha transilluminator and or perform other analysis Please note that he bands dics 29 mite COO Eune Electrophoresis E Gel System Trouble Shooting you fad Qut you deed a dient run than you tended sae below teyou ase at nd agen lst BE you are run then eov ble Check te gel and see the keding eis rumning the amount of ime remaining timmer to remind you to check on cernes on gel imme pre puse Remember cu one mode to another will eta the ran For Scamp i fo minute po chase ta 13 mantes o ued Te ge el 2 sopping onmi iege turns to a tended an a rapid beeping eus te Bosse sin Far Mode Press and ries Rebus torem Ray Mode Bai Mode caused one ot su Teilen Er ver Dekere casate Diecon Preis and apie es ose 3 tage 107 arcis un Adaptor Use oniy UL Listed Class Adaptor nuded IE T TT TT umm
4. E Gel Agarose 1 2 typical E Gel Powerbase FOTO Phoresis Fotodyne wrnerr a 7 ElectroPhoresis Elchrom Origins FlashGel ref Flectroblotter BBRBared by Bob Morison Instrumentation Specialist J ransilluminator CPLSMorson ina Original Sep 2008 Rev Apr 2012 Pme Electrophoresis ORIGINS by Elchrom Scientific Width 55 cm Depth 41 cm Height 22 cm Weight 18kg Gel compartment D 10 8 x W 27 8 Tho ORIGINS by Eichrom comprises THE most cost effective alternative to capillary electrophoresis better results for lower costs Zo Technical Features Integrated system for coolingihoating external water bath nor temperature amy probo required Integrated temperature control constant temperature of the running buffer independent of running time and voltage Integrated pump for buffer circulation guarantees constant temperature and eliminates pH gradients even in the vicinity of the electrodes Parallel double electrodos on either of the gel compartment provide a homogeneous and uniform electric field Working temperature range 4 C 55 Link to Elchrom ORIGINS Technical Manual pdf Link to Elchrom GEPS Power Unit User Manual Link to Elchrom Starter Kits for 2100U 110voll STLCC CPLSMoeison 4 2013 Paez Electrophoresis ORIGINS Maior Features Power Description 1 Onoff button
5. Gel Cutter Tool UVPBS 0002 01 Ultra Violet Products 85 0002 01 CPLSMorson Electrophoresis UV Transilluminator BRDG MINI UV TRANSILLUM TM 10 1154 21476 040 Supplier UVP Transilluminator Benchtop Model M 10E 6W Single intensity for detection of nucleic acids in all gel documentation applications filter decreases solarization UV blocking cover included s Wavelength 302nm UV Filter size 10x10cm E 114Hx26Wx18 4Dcm 1154 60Hz ETL ETLe certified On Off UV Blocking cover lid Analog dial set at detachable for cleaning 302 or 365nm GPLSMomison Page 29 Electrophoresis FOTO Phoresis Minivisionary System Camera Hood and Filters Receives power and commands from Minivisionary Controller Sends video signal to Thermal Printer tor copies Simple Inexpensive Thermal Prints No Computer Needed MiriVislonary Workstations are designed to simply and rapidly document electrophoresis gels in both research and educational laboratories These stand alone CCD based imaging systems allow the acquisition of very low light fluorescent gels Inexpensive thermal prints can be produced for about 1 10 the price of conventional fim with the touch of a button Fotodyne FOTO Analyst MiniVisionary FD62310 System Photodocumantation FOTO Analyst MiniVisionary Hood Mount System CCD camera Hood C
6. 2 Cables connection As to Power supply 3 Display a 4 Navigation for a ti Display E 1 4 Description of the back side 1 On Ott bution 2 Fuses AT 8 15 230 V AC 4 Serial number STLCC CPLSMoeison iota ERR EPDRCSCA QUATIT PT QURTENT TIUS buffer 28 above the unperelectoc Set up the butter o ap propriate temperature Du setting Te houc os OFF Ter perature changed by one ofthe artical anaw arc Saed oy pese ng SET button Select and prepare the gel or elcetrophorcoio EEror precast gel to ready bu orly roc t remove ham the packaging eno acanding o tableon page 10 Gut te aluminium on one site the plastic bag with the gelou aed cut the ries on 3 ses Pee she o Grip the gel wih forcopz astro plate backing overang Place the gel into the ORIGINS unit Turn cf the the gal wth lorcecs the laste overtang sire thal tapped reer he paste hacking or in tra sample wale Dna Web Mini Bl gok lom ono bag or Mim cami be run simultaneous y Catamaran Gel eer cote ino aaro yur gl a conor oston reap mone koorma era i
7. Taylor Angela BioLynx Sales BioLynx Quotations Boadeker Elizabeth Jennifer S Barban JoAnn L i Norris Richard J Morrison Robert G Staerk Becky Gaetano Denechaud Subject RE Biolynx Inc Set Up As New Vendor W9 Needed Good morning Angi BioLynx Inc is now the Distributor of Eichrom products in the United States We appreciate you contacting us Our Accounting Dept wii be sending an email with the information required to set up an account to purchase from us Please note the email address wil be chromspac com Chromatographic Specialties Inc is our sister company ng wil process your orders and send confirmation via email team would be pleased to provide you with any information you require For more information regarding 25 BioLynx Inc visit our website at www biolynx ca Lam a contact within the office to answer any questions you may have Sincerely Gadtane Denechaud Gaatane Denechaud Customer Service Coordinator Coordinatrice du Service la Cliente BloLynx Inc www blolynx ca 1 613 498 2126 ext 327 Toll Free in Canada Sans Frais au Canada 1 888 599 5969 gavtaned biolynca STLCC CPLSMoeison 4 2013 Electrophoresis E Gel System Powerbase V4 0 15 and 30 min Observe Status light per instructions on next page control buttons Power plug must fit securely in slot If status lights do not
8. show with an E gel in place the plug may be loose Use pliers to crimp the circular plug to more elliptical shape to tighten the grip in the slot Make sure both base pole electrodes are clean Scrape if necessary Secure E gel Agarose packet by inserting right side first then snap left side into place ETHROG or Invitrogen E Gel Power Base Ver 4 Invitrogen E Gel Power Base self contained unit with a adaptor that plugs directly into a wall Powerbase outputs 8 8 8 0VDC and maintains a current 0138 42 18 min and 30 min timed program so that it wil shut off automaticaly when electrophoresis is complete E Gel Pre Cast Agarose Gel Electrophoresis System Link to Egel Powerbase User Manual E Gel Agarose 1 2 typical Page 19 10 11 Electrophoresis E gel Basic Operations Before putting E gel cartridge in the powerbase attach the circular end plug of the power adapter cord into the hole in the base and then plug the adapter itself into the 110V Outlet A brief self test of flashing lights and beeping will occur then no lights until step 2 is completed Insert the E gel cartridge into the base right side first then press the left side down There should be an click sound as the cartridge snaps into place and a steady red light will appear Ready Mode No button pushes are required to reach this state A two minute pre run with the E Gel comb in place required before you w
9. 7 35 32 x 23 4 Thermo Sci BIA 27372 226 Mini Gel systems have a small footprint and can run 5 34 samples or 400 and 600mL systems or 8 48 samples for amp 00mL systems on ono gel The butfer chamber features lane visualization strips which aro sikscreened on tho platform to provide clear view of sample wells Lane markers and fluorescent rulers provide accurato fragment size determination of DNA when compared to known standards Durable acrylic construction and research qualty components give the buffer chamber a long life Platinum electrodes and gold plated banana plugs are corrosion protected and electrode placement ensures a uniform electric Leakresistant gasketed ultraviolet transmission rays allow tape free casting in the buffer chamber The trays have comb positions and allow DNA bands to be viewed on a transilluminator without removing the gel Optional multioad UVT trays have 12 Slots for multiple sample running and allow samples to be loaded directly from a 96 well late into an agarose gel This design is based on the fixed spacing required to accommodate multichannel pipats at least eight channels and 96 well plates Heavy duty one piece combs eliminate tno need for comb assembly The SuperSafe safety lid eliminates the risk of accidental shock System is supplied with a 0 91m 2 safety power cord attached STLCC CPLSMoeison 4 2013 Page 24 Electorphoresis Agarose Gel Guidelines Edvotek K
10. minutes or less Watch DNA migrate at your bench in real time without UV light Enjoy outstanding resolution and exquisite sensitivity The FlashGel System consists of enclosed disposable precast agarose gel cassettes and a combination of electrophoresis and transilluminator Cassettes contain procast prestained agarose gels and buffer need for gel preparation bufer addition gel staining The FlashGel Dock is an electrophoresis apparatus with a transilluminator that provides both separation and detection FlashGel Starter Kit includes FlashGel Dock FlashGel Cassettes 1 ml FlashGol Loading Dye 1150 pl FlashGol DNA Marker See Brochure for details Page 36 Electrophoresis E Gel System Quick Guide Te tabis is provided belou toe your convenience auier Operating modes and electrophoresis rune deze below You age the Reference guide tope o E Gab Venio Emm Acn Soi te iper No light Gf no cca Red m None Steady red ving tough ge base pe Pee nime Steady geen omi baton Tiens er iens Aves Besser Pacing et Sorun acan messes mede Es siue See Troubleshooting ext page
11. one piece Lighted display indicates when the two minute pre run is complete and whan to load samples See details Includes Power cord and manual Required Accessories Double comb or singla comb E Gels CPLSMorson Electrophoresis Typical Gel Setup Onon Voltage or Amps di control Typically to maintain 95 volts Red Electrical during run Digital Volt Pops expose Voor fetal and Amp readout expand thin strips Window Electrical Rocker Switch Set to constant Volts or Amps per lab procedure or start end of plate Typically 95 Volts Safety Notes 1 Connect all wiring Rad Electrical Probe goes to far or and probes before turning the unit on 2 Nevertouch the receiving end of plate probes or power supply box while the hia device is on fener eise 3 Tum off the device out expose the before disconnecting metalic portion and electrical probes expand the thin metal strips to Increase friction in the female socket 4 Do not handle the device with wet hands or on wet countertop 5 6 CPLSMorson ina Electrophoresis Typical Instructions and Guidelines Place the cover on the electrophoresis unit making sure to have the negative black electrode at the well e
12. 95 pris paper san Normal Size 33 Sac NTSC Sec PAL Side Vandsacpe Seo NTSC 8 4 Sec PAL Picture Nowa Approx 4 ches Picture Side 4 x52 inches Picture Size Square x inches Dual Power Supply AC 100 120 50 AC 200 240V He Power Consumption 1 51008 amos Dimensions I x Dx H6 38135 inches Weigh 6 16 Regulatory Approvals UL2601 1 C222 NeO FCC Class A TUV GS TUV GM EMC mode Fisher Scientific Catalog Paper Thermal Printer Fotodyne Approx 260 prints roll 3 x 4 in 7 6 x 10 2cm Accessory for MiniVisionary Printer FD602012 Fotodyne 60 2012 Electrophoresis Thermal Printer Paper Replacement 1 Press to open printer door 2 Locate KP65HMce thermal paper in the cabinet above printer Open foil and place in carousel with end at top of the roll 3 Insert new paper roll with shiny face on outside whiter flat face on inside of roll 4 Extend paper 2cm outside of cut edge 5 Close door and resume printing STLCC_GPLS Monson Varlable Intensity STLCC CPLS Morrson Electrophoresis PhotoDoc It System MIDSCI Cat 4 97 0274 04 PhotoDoc lt System w M 20V Transilluminator 20x20cm Digicam Color camera 10 0 megapixeis SD memory card 16 MB minimum Power cord included bateres not required
13. Darkroom UV blocking vier window Lightweight and compact Handles easy transport ol the hood Fits on top ofthe transiluminator Emission Fiters Er Red 570 6400 Transituminator The system is designed for use with UVP s Benchtop UV Transituminators Select trom 302 UV or 2UV model wih 3021m and Fiter sizes 20x20cm or 21x28em 20V and M 26V feature variable intensity setings Color Printer 300 x 300 resolution in prints USB connectivity 108 prts included Dimensions 7 x5 x25 in 178 x 127 x64 mm Combination Lock 3 dit resettable combination 2t cable Dimensions 17H x 13 25W x 65D in 432x337 x 241mm Pane 34 Electroblotter Semi Dry Ellard HEB 2020 The HEB 2020 accepts gels up to 20 cm x 20 cm Completely enclosed id and drip retaining base For safely power leads must be disconnected to open unit Color coded lid base and leads prevents reversal of polarity Solid construction of 3 16 inch red and 1 4 inch black 3 8 inch laminated base with 1 4 inch thick graphite platos Advantages of Horizontal Semi Dry Electro Bloter Transfer time one hour at constant 0 8mA square cm at room temperature Increases capacity to six gels Reduces Buffer Requirements to only 200 ml No cooling required Standard power supplies can be used Safety Cover STLCC GPLS Morison dots RELATED INFORMATION VWR Catalog Website Owi Series Semi Dry Electroblotters Thermo Scientific Supplier Thermo Scie
14. constantly measured and power calculated When one of the pre set parameters is exceeded the generator stops and will keep that parameter constant How important is the resistance of an electrophoresis unit The resistance of an electrophoresis unit depends on its size gel thickness amount of buffer buffer conductivity and temperature This resistance wil normally decrease in time due to a slowly increasing temperature Electrophoresis units which have resistance below the minimum load resistance of a power supply will trigger an alarm and or cause fuses to blow Read the output voltage and current during a run to measure the resistance and use above formula to calculate the value How to keep a constant voltage during a run Program the desired voltage and a higher current and power then the maximum expected values Current gt Voltage Resistance Power gt Voltage x Current How to keep a constant current during a run Program the desired current and higher voltage and power then the maximum expected values Voltage gt Current x Resistance Power gt Voltage x Current How to keep a constant power during a run Program the desired power and a higher voltage and current then the maximum expected values Voltage gt Current x Resistance Current gt Voltage Resistance STiCC CPLSMorson goa Page ss
15. e place vetore Inui your camper to esr pope ar our DNA regent 1 Pros and hold either button ushered ght a flashing green Might sitire fat tne Lut pres he dd 2 Athe ond ofthe pre automatically of The flashing ereen Tight wil change oa Mashing red ight he Peter vl beep rapidi Press and lease sae tn te Deep Neroni one Deap The ight eange gem a flashing ed igit gh Ramage te com em gel pli it ght ip Fora doublons pl encre De o7 gorily pesna aside her comb Remove any id ati prezent an ne vl Lond your samples into wel For move ample psp and Lending poser Running the 3 Alles Loading te gl hove om the appropriate conditions Gel ios depending onde Vou 2 Using the Veron 4 noose nun foe inge cmi IS aile rem for deti cant 1 For the 30 aminute cun press and release 3D anin button to tre 30 ute electrophoresis The tane oa stend areen o pres and release the 15 min baton ine light appears to indicate he begining ce saute Note Tae vins time ge may 15 on 17 incer or doubler and Caren e El go uty st of the ed of en ur The EG Poche Veios agis ln nih dig rd gj su apid beeping Press
16. e uomen ETSY urt Prec onte paste ace by oni cag IMPORTANT when Wide MiniS 4x25andS 4x13geks Thegolm ct sored such ia na cranral tai 4 25 5 13 sectors Inewi aorrirg ofthe Catamaran S2010 The orar Tow the h DNA tha upper aseton Electrophoresis ORIGINS Mode of Operation Running Precast at 20C Pgi of 2 Pages A ZE sarpe wee enough ro ainw tre Check the correct Catamaran positionts sides mus rest Tha plastic over ang in ihe carter f te cnarrbar enc not on the gel Sample wells nasci 1 be paralel to the secticces Electrophoresis ORIGINS Mode of Operation pg 2 2 Buller level should be about 2 3 irm above the upper platinum wire see rrarked level on the leit side Remove excacs butter with a 20 50 rl sylinge if necessary mploo Prior to loading ewitch off the Pump Loacicarnp oc Ont acing amp 4 5 The with of the opata tps Pages Electrophoresis ElChrom Power Unit Controls m 9 Link to Elchrom GEPS Power Unit User Manual pdf
17. ill load samples into the wells Press and hold either the 15 or 30 min button for a few seconds the steady red light turns into a flashing green light for the 2 minute run At the end of the the flashing green light will turn to flashing red and a rapid beep will sound Press and release either button to stop the beeps and return to a steady red Remove the comb from the E gel cartridge carefully lifting straight out and then clear up any gel residue near the wells Prepare and load your samples into the wells per the E gel manual guidelines For a single comb gel press the 15 min button a steady blue light will appear for the duration of the run For a double comb gel press the 30 min button a steady green light will appear You may interrupt the run at any time by pressing either button once and then again to restart but you must manually time the remaining portion to avoid overrunning the gel At the end of the run periods the steady light will return to a flashing red light and beeps will sound Press either button to stop the beeping and return the light to a steady red Remove the E gel carefully from the powerbase and you are now ready for the snc drensiliuminater and or other analysis Bands will diffuse within 20 minutes however Electrophoresis E Gel Rig BRDG Ethrog Biotechnologies Purchase through Fisher Scientific S11191ND Ethrog Biotechnologies No 65 0017 Abase and power supply together in
18. it Hotlink to Edvotek Guidelines for Gel Electrophoresis Pdf 24 pgs Electrophoresis Phase Lock Gel PLG Eppendorf Manual Acus Phase gt lt Aqueous Phase Organic Phase lt Phase Lock Barrier Phase Lock Gel gt spin Trapped Organic Phaso Plus Protein amp Contaminants Pl 4 4se Lock Gel PLG Hotlink to Eppendorf Phase Lock Gel manual Pdf 24 pgs STLCC CPLSMoeison Electrophoresis FOTO Phoresis Operation 8 6 x 10 8 cm viewing surface UV Blocking Cover with interlocking safety switch and indicator Perfect positioning of mini gels for Pholodocumentation Notched acrylic viewing trame for holding standard Poti plato Two S watt 300 nm bulbs Cooling fan Replacement Bulbs 200 nm 9 watt 5 7 8 pin o pin 11 2122 Interlock STiCC CPLSMorson inta Electrophoresis Transilluminator UVP LM26 Transilluminator UV Benchtop LM 26 20 Single Intensity 100 115V 8w Midrange Wavelength 302 365nm E Ballast Purple Base Stainless Steel Filter Assembly 21 x 26cm filter surface w UV blocking cover 9 50 x 13 25W x 4 758Pr Includes UVG 5000 hour long life filter and hinged UV blocking cover with adjustable height On Off UV Blocking cover lid detachable for cleaning Analog dial set at 302 or 365nm
19. le 5 10 mide protocol Go om sample acing o recovery in just ve minutas Recover samples drectiy trom tne casete witout band exci or purtieation Visualize sample recovery without UV Ig and recover at 0 100 Dock s an electrophoresis apparatus wih a Iha provides visualzafon of both DNA and RNA The FlashGel camera captures gel images the system righi at he benchtop wih complete gl un and image in hai minutos cases contain precast prestained agarose gels and butler wih no need or gel preparation aden or gel staining Ordering Information DNA starter kit includes the FlashGel Dock nine DNA cassettes with precast prestained agarose gels 1 2 agarose and butler in 12 1 well single tier format loading and DNA marker 100bp 4kb RNA starter kit includes nine RNase free RNA cassettes that fully enclose the gel 1 2 stain and running buffer in 12 1 single tier formaldehyde sample buffer RNA marker and molecular biology water Page 42 Electrophoresis Power Voltage Current Resistance What are the relations between Voltage Current Power and Resistance Power W Voltage V x Current Resistance 0 Voltage V Current A How does a power supply react affer pressing RUN The internal generator will start building up the high voltage at the output terminals while voltage and current are
20. m SESE meme Orne Prset tore Ried B wom Electrophoresis E Gel BRDG FlashGel Lonza Lonzaf VWR DNA Starter Kit 57026 95015 612 FlashGel systems consist ol enclosed disposable precast agarose cassettes and a combination electrophoresis and transilaminator un FlashGet DNA system separates DNA in 2 7 minutes wih outstanding resolution and allows monitoring of el runs right at the bench without UV ight The sensitive sytem also 5 20 mes raduction n DNA levels saving boh me and money RNA system is a fast and sente fool ior analysis of sample and opiized unique requirements of ANA Recommended lor variation and analysis tota ANA quei checks of nave RNA and checking RNA degradation and mRNA The system provides results m 30 minute or less avoiding usa o hazardous reagents and contaminating RNase RNA fragmen aro analyzed on the FlstGe system using Ihe same last and simple procedure used for DNA Separation complete n less than eight minces and ANA samples are ready or imaging within 10 20 minutas FlashGet system fa RNA 5 20 imes sensitivo than sthdum bromide ges lt 10ng per band are car detected conserving precious RNA samples system for DNA recovery eiminates agarose gel preparation and avers highly fient recovery ee from inhibitors and UV duced damage simp
21. nd of the gel Insert the electrode cords into the proper inputs of the power supply Set the power supply on low and turn it on Set the voltage at 90 95 volts Check to make sure bubbles are forming in the buffer on the platinum wire electrodes along the ends of the gel box Watch the gel closely for 2 5 minutes to make sure that the dye is migrating in the correct direction you hear an audible alarm or any of small red lights Fuse or gt 500 come during the run turn off the unit immediately and unplug The audible alarm and overcurrent lights indicate potential breakdown of the gel mixture or higher than normal temperatures in the gel For further information on Voltage Current and Resistance see the following slides If the Gel Rig Electrode probes pop out or become lose during the run Turn off and unplug the Power Supply and remove each electrode Inspect each electrode probe end by exposing the metallic portion Use a sharp tool to spring the thin strips on the metallic probe ends to create a better grip in the power supply female sockets Allow the tracking dye to migrate 4 5 centimeters from the wells so that the molecular weight marker fragments separate sufficiently Turn off the power supply unplug it and disconnect the electrodes from it Then remove the lid from the gel box on Electrophoresis Horizontal Mini Owl Series 400ML Mini Gel System 400 ML Size 16x 9 5 x B 65 16 x 334 x 28 32 8
22. no Eh E v mo tct op ues E Surge de wis ring depreme mense ot decer Elie yh url He sparo pra outset ae START sus wren censes he Leds dorky Boule eius srg fee nck Pe To ig Goreme mode bt at the ta perse ond rese vie Char Se ve Urale sperase LED NLT M LLARe Ihe rorreonaling cut set tess reaches ing the use of fern fe ne Sy ces i curl clue Me aeae paw MOCE e is dwing edet he of te 6 nen run hes ben conie depess cesse aput tare rue bete rover cos Fr he oe rt Tum Electrophoresis Elchrom Power Unit Output without Timer Mode 1 I We green LED on the left of the TIMER is aligniec depress Seto to the TER ten geen cn he lot che BR Electrophoresis 2 bers he CIAR iot cane es Elchrom Power Unit ren wien minutes desessing fe incendia and decens Output with Timer To range tho etingo une V m A chos inrosee me ela Mode bepress he START swich vidste START LED Tinte nd fe Leds WI diy ees Derereri m me ouput ew bee ono ot
23. ntific Rapidly transfer proteins or nucleic acid molecules trom polyacrylamide or agarose gels to membranes Reduce Southern Northern and Western batting to less than one hour Solid plat platinunvitanium and stainless steel electrodes are highly conductive and allow transfers at low voltages without external cooling systems Plate electrodes provide a uniform electric field for efficent even transfers Lid and attached power cord are safety nterlocked to prevent electric shock Lid is designed to ensure proper orientation of electric feld Minimal buffer is needed for accurate transfers the amount Contained within the blotting fiter paper is sufficient Easy to turn knobs allow uniform pressure to be applied across the entre blotting stack for even transfers Use electroblottor 27372 374 for mini to full size gels Unit is designed specificaly for the transfer from polyacrylamide or agarose gels to membranes Electroblotter 27372 378 is ideal forthe transfer of nucleic acids sequencing gols to nylon membranes Pages STLCC CPLSMoeison Cambrex FlashGel System The FlashGel System is the fastest way to separate DNA and the only to watch DNA migration as it happens This revolutionary new tool separates DNA in 2 7 minutes Monitor gel runs right at the bench without UV light The highly sensitive system allows a 5X reduction in DNA amount saving both ime and money Gat results in 5
24. ontroller Ethidium romido coomassie blue melhyleno blue fiters Thermal printer Cable Support frame 120V 60Hz Minivisionary Controller set copies then use this PRINT button Automatically sends commands to the Thermal Printer Gets commands trom controller PC or Minivisionary Has options for brightness and multiple copies Link to Fotodyne Brochure on Minivisionary pdf CPLSMorson igna Page 20 Electrophoresis FOTO Phoresis Focusing and Aperture on Camera CPLSMorson Focusing Adjust focusing and aperture on camera to align with markings on the dial as shown below A target of B seems to work well for the Fotodyne unit at BRDG Page it Electrophoresis MITSUBISHI P93W Monochrome Printer Copy Use ONLY if you want to set Multiple copies from each press the PRINT button on the Minivisionary Controller Paper replacement press to open door see next slide for details STLCC_GPLS Monson Pret Method Thermal pining on thermal paper Dot Density 325 dp Resolution NTSC 220 x 00 dts PAL 1280 x 600 Greyscale 2 ve input and ouput Memory 10 Fames mage ses Normal Side landscape Mut Normal Square 1 1 and expanded i 05020 Piin Capacty Normal Approx 280 prints paper Save made Side 150 prints paper save modo Square 1 1 Approx 1
25. rophoresis Elchrome Load Samples N Prepare 30 mM TAE buffer 1 Fill the apparatus with 1 9 1 30 mM TAE buffer 2 3 mm Electrophoresi above the upper electrode Switch the ORIGINS ON by ON OFF button Set the ElChrom Short Temperature to 9 Set Pump Delay to 12 min Manual GMS gels Set the Timo Every value has to be confirmed by pressing SET button at 9C Pg 1 2 Frob Wo Running Elchrom ready to use GMA gels POR roc 7M stock ston Mwet SSCP in ORIGINS hy Elchrom at D e B Heat ho amplos at 0550 far 6 min end quichy paco the tubes in water 3 mit When samples are roady take the gel 2 place in the ORIGINS by Elchrom and fix it with the catamaran frame Fead Switch the Pump and the Time OFF Load 9 ul of the samples quickly and close the lid Start Power Supply and Time on ORIGINS ON When electrophoresis is completed take the gel out using the forceps 5 and the needle Place the gel on the 1 and detach it from the plastic backing with nylon string for taster staining or blotting 6 Staining tray on the shaker 7 7 Page 18 Stain tho gel in the Electrophoresis ElChrom Short Manual GMS gels at 9C Pg 2 2 Running Elchrom ready to use GMA gels or SSCP in ORIGINS by Elchrom at 9 C Page 17 Electrophoresis Elchrom Gels Distributor Jan2012 Sent Monday Docembor 17 2012 1008 AM
26. the modes Lede MILLIAPS rine na carg ne parenetrconratmg Sikh of decreases ir eng or Pose corners ning the pogesses Trese charges romesent m Fe doctophocae rt cuo E anae dew he set ving he un depress READING swich HI decim We tutu set mies os Erg READING is depressed neo he TE ADING toss Lage vil jo curptect ice Kor Pree secos Pun sich base displaying he cta cup dues 5 ie pestie change ser elise wroutcezcesng te STCP change te output et values da the un te or devesse cor re recumolrg ani wives duina he couse we nan START Mie Fe sty sino thee MI St ce brat xac Vues ug seconds rd en swe beck 1 sta scil out omoota MOLE LED a Tara p VERTI air ate eu the E conlra porre nut ste M T To esmbich te tonstet mode Er ocelint cet Tu o ced fo dostod onc oft output t LED MILLIANPS Vues te set me lop fe capote suben
27. tnn CPLSMorson oth of sem eot Electrophoresis ElChrom Power Unit Controls CPLSMorson gota Maia Ferrovie Saree STEP START Sip STARTIed Led avenge node Lad Tius xece Led Voltage ices cras Cuin timer swatch CLEAR rich Trading sede Tensen at winder set trie ia nins mur Bega extat STOP mode STAR Turc geom ad or antec node vites set poirt Dexsesekecrezo oent eet point tue et nade Potties CORE s elem 3 of reviens nang Kid rezati Display ouput set vus Page 10 Electrophoresis Elchrom Prep of Precast Gels pg 10 11 of manual Electrophoresis Capillary Prep of Samples Pg 12 13 manual En ET my nnn San on ni te purus rer mnt ri ri at eminere tay sty Electrophoresis Elchrom Mode of Operation pg 14 15 Manual Ll STLCC CPLSMoeison iota Electrophoresis Elchrom Mode of Operation pg 16 17 manual en omani et ii Elect
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