SC-100A Manual
Contents
1. manual 3 Version 1 0c 9 Titrate the sample by adding the SO gt Titrant dropwise from the syringe or buret being sure to note the starting volume mark on the syringe or buret Try to accomplish the titration as rapidly as possible in 2 minutes or less but be careful near the endpoint so as not to overrun it here dispense one or two drops at a time Be sure to maintain stirring or swirling throughout the entire procedure 10 During the titration the LCD display will show transient currents the red STOP LED will briefly illuminate and the beeper will sound beep beep These transient indicators will last longer and longer as you approach the endpoint Take the endpoint as the first addition of Titrant that causes the display to exceed 50 and the red LED and beeper to stay on for longer than 15 seconds or a count of 20 beep beeps Itis important to maintain stirring or swirling to detect the endpoint well Read the endpoint volume off of the syringe or buret 11 The free SO gt content is calculated as 64 V N 1000 ppm SO 2 S where V mL Titrant needed to reach the endpoint N normality of the Titrant S mL sample If you use 25 mL of sample as directed and the Titrant is 0 0156 N as supplied then the calculation is simply ppm SO 20 V ie 20 times V For total SO 1 Place 25 mL wine or must in the titration vessel 2 Add 10ml 1N sodium hydroxide and mix well Let stand approximately 10 mi2. RANTIES IN THESE TERMS AND CONDITIONS ARE IN LIEU OF ALL OTHER WARRANTIES EXPRESS OR IMPLIED INCLUDING WITHOUT LIMITATION ANY WARRANTIES OF MERCHANTABILITY NONINFRINGEMENT OR FITNESS FOR A PARTICULAR PURPOSE SAID WARRANTIES BEING EXPRESSLY DISCLAIMED 2 Buyer agrees that its sole and exclusive remedy against Vinmetrica shall be limited to the repair and replacement of Materials or parts of Materials provided Vinmetrica is promptly notified in writing prior to the expiration of the warranty period specified above of any defect Vinmetrica s liability for any damages due Buyer shall be limited to the purchase price of the Materials 3 VINMETRICA S MAXIMUM LIABILITY FOR ALL DIRECT DAMAGES INCLUDING WITHOUT LIMITATION CONTRACT DAMAGES AND DAMAGES FOR INJURIES TO PERSONS OR PROPERTY WHETHER ARISING FROM VINMETRICA S BREACH OF THESE TERMS AND CONDITIONS BREACH OF WARRANTY NEGLIGENCE STRICT LIABILITY OR OTHER TORT WITH RESPECT TO THE MATERIALS OR ANY SERVICES IN CONNECTION WITH THE MATERIALS IS LIMITED TO AN AMOUNT NOT TO EXCEED THE PRICE OF THE MATERIALS IN NO EVENT SHALL VINMETRICA BE LIABLE TO BUYER FOR ANY INCIDENTAL CONSEQUENTIAL OR SPECIAL DAMAGES INCLUDING WITHOUT LIMITATION LOST REVENUES AND PROFITS HAZARDS AND TOXICITY All Materials offered by Vinmetrica are intended for use by individuals who are familiar with laboratory procedures and their potential hazards The Materials contain chemicals which may be harmful if misu
3. ary to be extremely accurate in this step with these pipettes 2 mL is roughly the amount that fills the bulb with a single thorough squeeze Caution the Acid reagent is corrosive and can cause damage to clothing skin and eyes The Reactant solution should not be ingested We recommend use of laboratory safety glasses and latex or nitrile gloves during this procedure If any solutions contact skin or eyes flush with plenty of water 5 If you are using a magnetic stirrer turn it on to stir at a moderate rate Be sure the stir bar will not strike the electrode in the next step 6 Rinse the electrode briefly with distilled water Insert the electrode into the titration vessel so that the tip is completely submerged to just above the circulation gaps cutouts at the tip of the electrode Ts If you are stirring manually begin now use a constant moderate swirling motion If the electrode is not held in a stand hold it against the side of the vessel with one finger and grasp the vessel with the remaining fingers so that the two move together Figure 2 Figure 2 Manual stirring technique Hold the electrode against the side of the vessel and swirl gently add titrant with other hand 8 Verify that the current is less than 50 and the green PROCEED LED is lit If the current is greater than this and or the red STOP LED is lit and the buzzer sounds your sample has less than 2 ppm SO and there is no need to proceed SC 100A
4. ay but continues to operate without impairment to any function Replace the batteries as soon as practicable When the battery level drops too far the instrument does not operate It rapidly flashes the low battery icon for 3 0 seconds beeps and shuts itself off Auto Shut off The SC 300 shuts off after 30 minutes If this happens unexpectedly just press the POWER button to resume from where you were 2 Attach the SO electrode via the BNC connector protruding from the top Remove any protective cover from the electrode tip some electrodes are shipped without one Put the electrode on its side or hang it from an electrode stand if you have one The SO electrode is sturdy with its plastic housing but do take care not to let things touch or strike the platinum wires they are somewhat fragile and will break if bent and straightened repeatedly Electrode care when done always rinse with DI water and let air dry There is no need to store the electrode in any kind of solution and the protective cap put on for shipping only should not be replaced OPERATION Turn on the instrument by pressing the POWER button briefly Note depressing the POWER button longer than two seconds at start up will cause the instrument to enter Test Mode see Appendix The instrument will go through a power up sequence After a few seconds the instrument will start You should see the display show a value less than 50 usually 0 0 and the green Proceed LED sho
5. bi Vinmetrica SC 100 AT M Oinmetrica User Manual The Vinmetrica SC 100A is a simple and robust device that provides high accuracy in determination of sulfite SO2 levels in wine These are essential parameters to control in the effort to make high quality wines Theory of operation Sulfite SO2 The SC 100A with the SO electrode and reagents provided can be used to determine sulfite or SO2 levels in wine musts and other samples It relies on the Ripper titration based on the quantitative reaction of the SO with iodine generated during the titration which oxidizes the SO in the sample under acid conditions IO 51 6H gt 31 3H2O generation of iodine from iodate SO b H20 gt 27 S03 2H reaction of SO and iodine When all the SO is titrated at the endpoint excess iodine appears in solution This is detected as current with the SO electrode and signaled by audible and visual indicators The endpoint is much more sensitive than the starch color change commonly employed for Ripper titration and it is sharp and clear even when titrating red wines and musts From the known concentration of the titrant and its volume required to reach the endpoint the free SO is simply calculated Materials provided in the kit Vinmetrica SC 100A SO controller unit Part number SC100A 1 SO Electrode Part number SC100 3 blue polycarbonate housing SO Reagent set Part number SC 100 2 SO Titrant so
6. ired Description 0 Version None The version number of the instrument software is displayed The instrument goes through a 1 Burn in None continuous burn in cycle exercising sound LEDs and display 2 NA NA Not used by the 100A Red LED flashes and display shows blinking 3 SO SOz probe or SO After a brief pause the SO current in probe simulator e g nanoamperes is displayed For values 450 kOhm resistor under 10 one decimal place is shown 4 NA NA Not used by the 100A display shows 000 5 SO Voltage SO probe or The raw voltage output from the SO probe simulator instrument s current amplifier is displayed as X XX volts XXX if less than 1 00 6 DAC Test None The Digital Analog Converter DAC is Disconnect probe cycled through its 32 levels 7 Battery Install two AA The battery voltage is displayed as X XX Voltage batteries volts SC 100A manual 6 Version 1 0c Section Equipment Required Description 8 Character Set None Every ASCII character space to is displayed Due to the limitations of the 7 segment format some characters are not used by the software 9 Number None The display cycles through showing Display every possible digit and every decimal point 10 Sound Test None The beeper is turned on continuously SC 100A manual Version 1 0c
7. lution 0 0156N Acid Reagent Reactant solution One 5 mL syringe Two 3 mL polyethylene transfer pipettes One 25 mL serological pipette One 100 mL polypropylene beaker Se ie Figure 1 The SC 100A US and international patents pending SC 100A manual 1 Version 1 0c Things you will need 1 Two standard AA batteries alkaline type 2 Distilled water it s handy to have a wash bottle or similar for rinsing see Vinmetrica product number SC100 17 at http vinmetrica com wine analysis products _ 3 Optional magnetic stirrer and stir bar electrode stand 10 mL glass burette and lab support stand Available from Vinmetrica http vinmetrica com glassware lab support stands magnetic stirrers 4 Optional Sodium Hydroxide solution 1N concentration if you want to do total SO2 Available from Vinmetrica Part number SC100 7 http vinmetrica com wine analysis products Setting up the instrument 1 The SC 100A Figure 1 runs on two standard AA batteries alkaline cells recommended To insert the batteries open the battery housing on the bottom of the back of the unit by removing the two screws and gently prying off the lid Install the batteries as shown by the and markings then close the housing If desired you can prop the unit up using its folding stand Low Battery Detection When the battery level is getting low the instrument shows a low battery icon on the upper left side of the displ
8. nutes 3 Using the transfer pipettes add 8 mL Acid Reagent and 2 mL Reactant solution to the vessel Remember that if you are using the transfer pipettes in the kit 2 mL is the amount that fills the bulb with a vigorous squeeze so dispense four of these for the Acid Reagent 4 Proceed from step 5 in the Free SO2 procedure above The result calculated will be total rather than free ppm SO Finishing up l Turn off the instrument 2 Rinse the SO electrode and syringe with distilled water Let air dry 3 Store all reagents tightly capped and away from heat and sunlight 4 Discard waste samples and solutions in accordance with local regulations Acidic solutions can be neutralized by slow addition of baking soda sodium bicarbonate with stirring until effervescence ceases Technical assistance info vinmetrica com tel 760 494 0597 see also our FAQ section at www vinmetrica com 64 mg SO mmol SO V mL N megq mL 1000 mL L 2 meq mmol SO S mL SC 100A manual 4 Version 1 0c WARRANTIES AND LIABILITIES 1 The materials provided in the kit as described on pages 1 and 2 above Materials are warranted as follows The SC 100A instrument electrode and non reagent accessories are warranted against defects in workmanship for 12 months from date of purchase The reagents are warranted to perform as described herein up until any stated expiration date or 6 months after purchase whichever is later THE WAR
9. sed Due care should be exercised with all Materials to prevent direct human contact Keep out of reach of children Vinmetrica 1945 Camino Vida Roble Suite Carlsbad CA 92008 www vinmetrica com 760 494 0597 info vinmetrica com Copyright 2010 12 Sportsman Consulting LLC DBA Vinmetrica All rights reserved SC 100A manual 5 Version 1 0c Appendix Test Mode Test Mode provides various special functions that may be useful in testing the device for example if troubleshooting is necessary Test Mode is entered when the POWER button is pressed longer than 2 seconds while turning the instrument on Remove the pH electrode if it is attached Test Mode is organized into sections Press the POWER button briefly to step to the next section After the last section Test Mode restarts the first The Stop LED red is illuminated when an error is detected by the instrument The Proceed LED green is illuminated to indicate no error detected The green LED does not guarantee proper functioning it only indicates that no problem could be automatically detected The user should make careful observations to discern proper operation To exit Test Mode hold the POWER button down 5 10 seconds until the instrument shuts off If the device does not shut off after 10 seconds of holding down the button move to the next section by releasing then pressing again the POWER button briefly then try to exit again Section Equipment Requ
10. uld be on Initial use The first time you use your system a new electrode may need conditioning to settle down We recommend you place the electrode in about 30 mL of distilled water and add about 2 mL of the acid solution as in step 4 below With the instrument on and electrode attached allow the system to equilibrate for 10 15 minutes By that time the current should be below 50 and the green Proceed LED should be on If not allow the electrode to sit in the solution for up to 4 hours the instrument can be turned off during this time If after this time you still don t get the Proceed condition contact us for assistance SC 100A manual 2 Version 1 0c Procedures Free SO 1 Make sure the SO electrode is attached Turn on the instrument 2 Fill the syringe by drawing up the SO2 Titrant Expel bubbles and set the plunger on the syringe to a readable point preferably the 5 0 mL point Note the 5 0 mL setting allows determination of up to 100 ppm SO in a standard 25 mL wine sample If you are using a buret instead fill it now 3 Place 25 mL wine or must in the titration vessel We recommend using the 25 mL pipette provided in the kit draw sample up to the 0 mL mark then dispense the sample into your titration vessel by letting the tip of the pipette touch the side of the vessel while the sample drains 4 Using the transfer pipettes add about 2 mL Acid Solution and 2 mL Reactant solution to the vessel It is not necess
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