TS-DW - User manual
Contents
1. Fig 5 Control pane and corrected values 6 Specifications The unit is designed for operation in cold rooms incubators and closed laboratory rooms at ambient temperature from 4 C to 40 C in a non condensing atmosphere and maximum relative humidity 80 for temperatures up to 31 C decreasing linearly to 50 relative humidity at 40 C 6 1 Temperature specifications e Setting range sista de eA Aaa A 25 C to 100 C COnttOl FANGS ssrt nera tae 5 C above RT to 100 C Setting reSOlUtion eee cee ececcee cece ceceeae cee eeeeeaeeeceeeeseeeeeeseeeeaeeeeeseees 0 1 C Stability at 37 C ieoi onceng i 0 1 C e Maintaining accuracy at 37 C oo ee see eneeeeeneeteeeeeeneeeeenaes 0 5 C e Uniformity over the platform at 37 C aiiin 0 1 C e Average heating speed from 25 C to 100 C eee ceeeeeeereeees 4 C min e Time of platform heating from 25 C to 37 C woe eeeenteeteeteeeeneeeeee 4min e Temperature calibration option e Calibration coefficient range eee ceeeeteeeteeesteeeenees 0 936 1 063 0 063 Data for 75 filled microplates For B 2E Eppendorf thermoblock Other block specifications are different 100 Temperature C 0 00 0 30 1 00 1 30 2 00 2 30 3 00 3 30 4 00 4 30 Time h mm Fig 6 Kinetics of liquid heating inside a single cell in a plate total volume 1000 mcl 12 6 2 General specifications Speed range siise irnia A R PN EEEa2. from the device Remove the four knurled screws lift the block without damaging the cable and disconnect the plug fig 1 1 Select the new thermoblock and install it according to the point 3 4 4 Operation Recommendations during operation A 4 2 Please check the deep well plates before using be sure that plates are heat resistant resistant Don t heat the deep well plates over the melting point of the material they are made of It is recommended to fill wells up to 75 of the rated volume for efficient mixing Caution Hot surface Platform surface becomes very hot during use Always use protective cotton gloves to install or remove test plate when set temperature is higher than 60 C Connect external power supply unit to a grounded power socket and set the power switch located on the rear panel of the unit to position ON The display will turn on with the upper line Set showing time speed and temperature set earlier and the lower line Actual showing current readings of the same parameters platform temperature C which automatically starts rising according to the temperature set in the upper line The time of temperature stabilisation depends on the initial temperature Setting the parameters 4 3 4 4 4 5 Use the readings in the upper line of the display Set while setting the required parameters Setting time TIME Using the A and W keys Fig 2 1 set the required working time interv
3. 250 1400 rpm Speed setting reSOlUtion s sies p a a aeii 10 rpm Maximal speed deviation OCZ SONO Meon a E E EE a AEE R tae 2 FOF TAOO g o an PNE AEE TAA A TT 0 7 ORME E E E E A A TE AT 2 mm Digital time Setting ccnercnie eee ien 1min 96h Time Setting reSOlUtion sisine aap eotea 1 min Maximal continuous operation time ccccececeeeeeeceeeeeeeeeceeeeeeeeeaeeeeeeeeea 96h recommended interval between operation sessions not less than 8 hours DIS PAY wie E E E E E A E 16x2 symbols LCD DIMENSIONS aieeaa a a EE 240x260x160 mm Input current power CONSUMPTION seeeeeeeeeierereerereeeenea 12V 3 7 A 45 W External power supply unit in AC 100 240 V 50 60 Hz out DC 12 V WeIQHt 3 E iets inate A Goths anak avait E 5 1kg for Eppendorf deep well 96 1000 ul microplates Block parameters is in section 6 specification B 2S for Sarstedt MegaBlock deep well microplates 96 x 2 2 ml wells Temperature maintenance stability at 37 C Temperature maintenance precision at 37 C Uniformity over the platform at 37 C Replacement parts Description Catalogue number Rubber belt 122x6x0 6 mm BS 000000 S18 Accurate within 10 Biosan is committed to a continuous programme of improvement and reserves the right to alter design and specifications of the equipment without additional notice Tafi 7 2 7 3 7 4 8 1 8 2 8 3 8 4 Maintenance If the unit requi
4. D Medical Biological Research amp Technologies Thermo shaker for deep well plates TS DW Sa Operating Manual for version Certificate V 2A01 Contents Safety Precautions General Information Getting Started Operation Calibration Specifications Maintenance Warranty and Claims CON OA RF WN gt Declaration of conformity 1 Safety precautions The following symbols mean A Caution Make sure you have fully read and understood the present Manual before using the equipment Please pay special attention to sections marked by this symbol N Caution Hot surface Platform surface becomes very hot during use Always use protective cotton gloves to install or remove test plate when set temperature is higher than 60 C GENERAL SAFETY Use only as specified in the operating manual provided The unit should be saved from shocks or falling The unit must be stored and transported in a horizontal position see package label After transportation or storage keep the unit under room temperature for 2 3 hrs before connecting it to the electric circuit Use only cleaning and decontamination methods recommended by the manufacturer Do not make modifications to the design of the unit ELECTRICAL SAFETY Connect only to the external power supply unit with voltage corresponding to that on the serial number label Use only the external power supply unit provided with this product Ensure that the power switc
5. al in hours and minutes increment 1 min Pressing the key for more than 3 sec will increase the increment rate Setting speed RPM Using the A and WV keys Fig 2 2 set the required speed increment 10 rpm Pressing the key for more than 3 sec will increase the increment rate Setting temperature T C Using the A and W keys Fig 2 3 set the necessary temperature increment 0 1 C Pressing the key for more than 3 sec will increase the incrementrate 00 00 1400 25 0 set A a a STOP 0000 25 0 Actual hr min RPM T C permo Smeer TS DW TIME RPM T C RUN STOP bioSan Fig 2 Control Panel A Caution Heating temperature maintenance process does not stop when the timer is finished Platform thermal regulation can be turned off only by setting the required temperature below 25 C the display will show OFF T C set point In this mode TS DW can be used in the cold rooms as a mixing device without thermoregulation Settings can be changed during operation Program execution 4 6 4 7 4 8 4 9 4 10 4 11 4 12 After the thermal stabilisation of the Thermo Shaker when the set and current temperature readings become the same Place the deep well plate on the platform and close the lid Press the RPM RUN STOP key Fig 2 4 The platform will start rotating and the timer indicator will start counting up the time interval with 1 min precision Note If the rotation speed is set to z
6. ating PSU 20i Multi RS 60 os arai Bio RS 24 Cell growing ass PF NEW nprs TS DW yi ja P Applications wo es ES Microbiology gt a oe Extraction N PST 60HL s A Cellgrowing NTS X with heating RIG gt PSU 101 Applications EED HA ane _ D lt A Hybridization MSV 3500 PSU 2T ES 20 _ NEW with heating Applications MPS 1 oom A 5 DNA analysis oe ts No hake Genome sequence 4 MR 1 lt 3 Applications ey Agglutination NEW Ly Extraction Multi Bio 3D CvP 2 A a Gel staining _ _ a destaining _ oer aces e a E J x E TS 100 with heating Go gt 4 TS 100C with heating AS Cr and cooling m J a i V 32 MR 12 Applications Agglutination wag gt Extraction e NS Blot hybridisation w Gel staining destaining a amp a gt EMI _ Level of liquid 10 10 ml 10 mi 10 10 ml Erlenmeyer flasks and Cultivation flasks Petri dishes vacutainers and tubes up to 50 ml PCR plates microtest plates and Eppendorf type tubes
7. ce of the set and actual temperature in the plate wells Features of TS DW meet the highest expectations of users according to many parameters Fast reaching of specified mixing speed and maintenance of equal amplitude of rotation throughout the Thermo shaker block Stability of maintaining the set temperature in a wide range throughout the Thermo shaker s block surface With the help of the temperature calibration function the user can calibrate the unit approx 6 of the selected temperature to compensate differences in the thermal behaviour of plates from different manufacturers LCD display indicates set and current values of temperature speed and time of operation Quiet motor operation compact size prolonged service life TS DW was designed using the multi system principle which allows using it as three independent devices Incubator Plate shaker Thermo shaker The device can be used in cytochemistry forinsitu reactions immunochemistry forimmunofermentative reactions biochemistry forenzyme and protein analysis molecular biology for nucleic acids isolation 3 1 3 2 Getting started Unpacking Remove packing materials carefully and retain for future shipment or storage of the unit Examine the unit carefully for any damage incurred during transit The warranty does not cover in transit damage Complete set Thermo shaker set includes Standard set TS DW Thermo Shaker for de
8. d in the event that warranty or post warranty service comes necessary Complete the table below and retain for your records Model TS DW Thermo shaker for deep well plates Serial number Date of sale 14 9 Declaration of conformity Declaration of Conformity Equipment name TS DW Type of equipment Thermo Shaker for deep well plates Directive EMC Directive 2004 108 EC Low Voltage Directive 2006 95 EC RoHS 2011 65 EC WEEE 2002 96 EC amp 2012 19 EU Manufacturer SIA BIOSAN Ratsupites 7 build 2 Riga LV 1067 Latvia Applied Standards EN 61326 1 Electrical equipment for measurement control and laboratory use EMC requirements General requirements EN 61010 1 Safety requirements for electrical equipment for measurement control and laboratory use General requirements EN 61010 2 010 Particular requirements for laboratory equipment for the heating of materials EN 61010 2 051 Particular requirements for laboratory equipment for mixing and stirring We declare that this product conforms to the requirements of the above Directive s a or lt O TE Signature Svetlana Bankovska Aleksandr Shevchik Managing director Engineer of R amp D 12 QS 2014 ALOS 1014 Date Version 2 01 December 2014 om R m wae wir eee we Bio RS 24 CEB e PST 60HL 4 PST 100HL Applications with heating with heating ES 20 60 ah b with he
9. ep well plates ee eeeeeeeeeeeteeeenees 1 pce external power supply UNIt cece cece ce eeeeeeeeeeeeeeseceeeeeeseeaeeeeeeeeeeeeeeeees 1 pce POWON CONG ioira she cendelel aed eecpletd din dates ceaapeseenlhaan dete liaandanenee 1 pce Spare TUDDEN belt rner esau ier aa egea ena E e aE an Ea ani 2 pcs Operating Manual Certificate eee eee ener ananena aa 1 pce Optional accessories thermal block 2E for Eppendorf deep well plates eee on request thermal block B 2S for SarstedtB deep well plates 0 00 on request Setup place the unit upon even horizontal non flammable surface 30 cm away from any flammable materials remove protective film from the display plug the external power supply unit into the socket at the rear side of the unit connect the power cord to the external power supply unit Thermoblock installation if thermoblock is not installed Caution Thermoblock installation and replacement have to be performed only when the Power switch is turned off and external power supply is disconnect from the device Choose the thermoblock connect the plug to the contact terminal according to the scheme on Fig 1 1 on the underside of the thermoblock Make sure that the connector is mounted tightly Align the thermoblock so that the connector pins are facing the right side of the unit Secure with the four knurled screws Fig 1 Thermoblock connection Changing blocks Disconnect the external power supply unit
10. ero pressing RPM RUN STOP key will start the timer but the platform will not move After finishing the program after the set time elapses the platform motion will stop and the timer will show the flashing reading STOP accompanied by the repetitive sound signal until the RPM RUN STOP key is pressed If the working time is not set or is reset and the timer indicator in the upper line shows 00 00 pressing the RPM RUN STOP key will start continuous operation of the device with countdown timer in the lower line Actual until the RPM RUN STOP key is pressed again If required there is possibility to restart the timer when it is running Press the TIME RUN STOP key once Fig 2 5 to stop the timer Press the TIME RUN STOP key again to restart the timer The platform motion can be stopped at any time by pressing the RPM RUN STOP key In this case the program realisation and the platform motion will stop and the timer will switch into the STOP mode saving previously set time Press the RPM RUN STOP key to repeat the operation with the same time and speed Caution At the end of the set time period the platform movement is stopped automatically but the heating can be stopped only manually by reducing the temperature using the Y T C key Fig 2 3 lower button till the OFF sign appears in the upper line Set of the display Caution When lid is open the platform and lid heating surfaces will remain hot Please take necessary care and
11. h and external power supply are easily accessible during use Do not plug the unit into an ungrounded power socket and do not use an ungrounded extension lead Disconnect the unit from electric circuit before moving Disconnect the external power supply unit from power socket to turn off the unit If liquid penetrates into the unit disconnect it from the external power supply unit and have it checked by a repair and maintenance technician Do not operate the unit in premises where condensation can form Operating conditions of the unit are defined in the Specifications section DURING OPERATION Do not leave the operating unit unattended Do not impede the platform motion Do not operate the unit in environments with aggressive or explosive chemical mixtures Please contact manufacturer for possible operation of the unit in specific atmospheres Do not operate the unit if it is faulty or has been installed incorrectly Do not use outside laboratory rooms Do not check the temperature by touch Use a thermometer BIOLOGICAL SAFETY It is the user s responsibility to carry out appropriate decontamination if hazardous material is spilt on or penetrates into the equipment 2 General information Thermo shaker TS DW is designed for shaking deep well plates in the thermostatic regulation mode A distinctive feature of BioSan plate thermo shakers is the patented two side plate heating that allows to achieve full corresponden
12. ng parameters will be shown on the display Fig 4 set emperature Set p 00 00 1000 40 Actualp STOP 0000 40 0 hr min RPM TC ammperal 7 re Fig 3 Control panel in operation mode 5 10 A 5 11 5 12 Use the temperature with multiplier coefficient readings to set the new temperature value Using the Temp A and W keys Fig 2 3 change the calibration coefficient Fig 5A 1 so that the new temperature value Fig 5A 2 corresponds to the autonomic sensor temperature In our example the calibration coefficient will be 0 974 in range 0 936 up to 1 063 increment 0 001 Caution This calibrating coefficient will correct temperature through all the operation range After finishing the calibration press the RPM RUN STOP key Fig 2 4 once to save the changes and exit the calibration mode The display will show calibrated temperature as shown on Fig 5B 3 and the unit will continue thermal stabilization according to the previously set temperature calibration D inner sensor set mode temperature temperature sct 40 0 40 0 1 000 40 0 40 0 1 calibration 3 modified J actual temperature multiplied coefficient Y temperature by previous coefficient Fig 4 Control panel in calibration mode set temperature A E 40 0 40 0 iai 00 00 1000 40 0 0 974 39 0 40 0 STOP 0000 39 0 i ibrati modified calibrated calibration I ifi O 3 eens coefficient temperature
13. res maintenance disconnect the unit from the electric circuit and contact Biosan or your local Biosan representative All maintenance and repair operations must be performed only by qualified and specially trained personnel Standard ethanol 75 or other cleaning agents recommended for cleaning of laboratory equipment can be used for cleaning and decontamination of the unit Rubber belt replacement For maintenance of reliable operation of the device the producer recommends to replace rubber belts after 1 5 years or 2000 hours of operation time Disconnect the external power supply unit from the device Remove 4 fixation screws on the device bottom and remove the bottom plate Replace the rubber belt Fig 7 Re assemble the device Fig 7 Rubber belt replacement Warranty and Claims The Manufacturer guarantees the compliance of the unit with the requirements of Specifications provided the Customer follows the operation storage and transportation instructions The warranted service life of the unit from the date of its delivery to the Customer is 24 months Contact to your local distributor to check availability of extended warranty If any manufacturing defects are discovered by the Customer an unsatisfactory equipment claim shall be compiled certified and sent to the local distributor address Please visit www biosan lv Technical support section to obtain the claim form The following information will be require
14. use protective cloth gloves at temperatures over 60 C After finishing the operation set the power switch located on the rear panel of the unit in position O Off and disconnect the external power supply from electric circuit 5 1 5 2 5 3 5 4 Calibration The device is pre calibrated at the factory calibrating coefficient is 1 00 for operation with temperatures measured by a sensor installed in the heating block To enter the calibration coefficient hold the TIME RUN STOP key Fig 2 5 pressed for more than 8 s to activate calibration mode The calibration coefficient will be shown on the display Fig 4 1 Set 1 000 value using the A and W keys Fig 2 3 as shown on Fig 4 1 to restore the factory settings Press the RPM RUN STOP key once to exit the calibration mode Calibration procedure 5 5 5 6 5 7 5 8 5 9 10 Install autonomic sensor 0 5 C accuracy into the plate well placed on the platform Close the lid Set the required temperature in operation mode e g 40 C After the unit reaches the set temperature when the set and current tempe rature readings equal leave the unit for 30 min for thermal stabilization Let us assume that the readings of independent sensor is 39 C but the display s actual temperature is 40 C Fig 3 Then it is necessary to add 1 C correction Hold TIME RUN STOP key Fig 2 5 pressed for more than 8 s to activate calibration mode The followi
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