EDP™ - Pipette Supplies
Contents
1. 105 130V 50 60Hz for domestic models A totally discharged Power Pack will be completely charged in approximately 14 hours A more elegant way to charge the EDP Power Pack is to use the accessory Rapid Charge Stand The Stand provides a convenient resting place for the EDP pipette Whenever the instrument is placed on the stand charging occurs automatically via the special external contacts on the sides of the drive module While the instrument is being charged on the Stand the symbol FC will appear on the display and all functions are inoperative Operation may be resumed as soon as the instrument is removed from the Stand The Rapid Charge Stand delivers current to the Power Pack much more rapidly than the wall transformer A totally discharged Power Pack can be completely charged in 1 1 2 hours Special circuitry in the Stand meters current and monitors the temperature of the Power Pack battery via a temperature probe contained in the Power Pack itself The Stand delivers current under carefully controlled conditions which assure that damage to the Power Pack will not occur When the Power Pack is fully charged the red rapid charging light goes off and the Rapid Charge Stand delivers a small maintenance current that allows the EDP pipette to be safely left on the stand for as long as desired 24 FIGURE 11 1 CONNECTING THE WALL TRANSFORMER 12 DISPOSABLE TIPS The requirements for dispos2. For these reasons operation with the Power Pack installed is recommended With the Power Pack installed the electronic drive module should always be connected to a charger when not in use This will assure that the Power Pack is well charged and minimize instances of discharge during use If the instrument will be stored without using for an extended period the Power Pack should be fully charged and then removed This will prevent discharge during storage Whenever the Power Pack is installed the drive module is on Even though the actuator is not moving the piston there is a continuous small power consumption by the micro computer electronics If the Power Pack becomes discharged during operation of the instrument the drive module will emit an audible warble and the message Lob will appear in place of the volume on the numeric display Whenever this occurs the instrument should be attached to a charger immediately after completing the current pipetting cycle If the wall trans former is used operation of the instrument may continue without interruption after a few seconds The Portable Power Pack can only be recharged while it is installed in an EDP drive module 23 Recharging is Simple With the wall transformer simply plug the transformer cord into the receptacle on the drive module Be sure the transformer is plugged into an active AC recepta cle which supplies power matching the specification on the charger
3. nominal volume range Each model consists of an electronic drive module and a replaceable liquid end All six models incorporate the same electronic drive module The liquid ends are interchangeable and may be purchased separately Each liquid end contains a precisely machined and polished chrome plated stainless steel piston which moves up and down during volume measurements The distance moved determines the volume measured The chamber below the piston connects to a disposable polypropylene tip via a column of air The disposable tip is the only part of the instrument touching the liquid Tips are easily changed between samples to avoid carryover The drive module controls the movement of the piston Power for movement is supplied by a special electronic linear actuator An on board microcomputer on a chip monitors the position of the piston and sends commands to the actuator The position of the piston at the beginning and end of each stroke is controlled solely by the microcomputer There are no mechanical stops The microcomputer communicates operating parameters and status to the user via a liquid crystal display and a tone generator It receives commands from the user via a 13 key keyboard and is activated by a convenient pistol grip trigger Power for the EDP pipette can be supplied either via a lightweight cord from a miniature wall transformer power supply or for fully portable operation from the optional Portable Power Pack acces
4. the volume indicator will change to display this smaller volume The last aliquot is normally discarded Q 13 What happens when dispense in the TITRATE mode A First if a non zero value was set for V2 the V2 symbol will appear on the volume indica tor and this volume will be dispensed quickly the first time the trigger is pulled Next or if V2 was zero pulling the trigger will cause the piston to start moving downward slowly If the trigger is held in the rate of movement of the piston will accelerate gradu ally increasing the rate at which liquid is dispensed until a maximum rate is reached You can feel this in the movement of the actuator as you operate the pipette If the trigger is released then pressed again the sequence of events repeats First slow movement then gradual acceleration to a maximum This allows you to approach an endpoint rapidly by holding the trigger or very gradually by tapping the trigger so that the piston always moves downward very slowly 20 At all times during the dispense cycle the volume display records the total volume dispensed In titrating to an endpoint it is always best to dispense with the end of the disposable tip actually immersed 2 3 mm into the liquid in the receiving vessel This promotes immedi ate mixing and avoids endpoint errors Q 14 How can empty the remaining liquid from the tip rapidly after dispensing only a portion of the aspirated volume in the MULTIDISPENS
5. 5 LIQUID MEASUREMENT MODES en 6 QUICK REFERENCE GENERAL ee 7 QUICK REFERENCE PIPETTE MODE 8 QUICK REFERENCE MULTIDISPENSE MODE 10 QUICK REFERENCE TITRATE MODE 12 QUICK REFERENCE DILUTE MODE ee 14 QUESTIONS AND ANSWERS eee 16 CHARGING THE POWER PACK eren 23 DISPOSABLE TIPS ns serene ddorien ed 25 HINTS FOR PROPER PIPETTING ee 27 CHANGING LIQUID ENDS nenn 29 REMOVING THE TIP EJECTOR ee 31 CARE AND CLEANING OF LIQUID ENDS 32 TROUBLESHOOTING eeen 35 PERFORMANCE SPECIFICATIONS 36 ORDERING INFORMATION eee 37 WARRANTY INFORMATION ee 39 1 DESCRIPTION RAININ EDP Electronic Pipettes measure liquid volumes from one fo 2500 microliters accurately and precisely Volume measurements may occur in any of four different modes PIPETTING DISPENSING TITRATING and DILUTING Trigger Drive Module Liquid End Disposable Tip FIGURE 1 1 DRIVE MODULE TRIGGER LIQUID END DISPOSABLE TIP Encoder Plug Display Keyboard Tip Ejector Button FIGURE 1 2 ENCODER PLUG DISPLAY KEYBOARD TIP EJECTOR BUTTON Six EDP models have nominal volume ranges of 10 25 100 250 1000 and 2500 microliters respectively Actual volumes are adjustable and may be changed rapidly and effortlessly by simple keyboard entries The recommended working range for each model is 10 to 100 of the
6. E or TITRATE mode A Press the key sequence F I O This will cause the piston to immediately move down emptying the tip Then after a brief pause the instrument will execute a blowout cycle and the piston will return to the home position and be ready to pick up the next sample Q 15 What is the purpose of the air segment in the DILUTE mode A It prevents contamination of the sample in its original container by diluent If you wish you can wipe the outside of the tip after the air segment stroke before aspirating the sample Q 16 Can the EDP pipette be used for reverse mode pipetting of serum plasma and other liquids of moderate viscosity A Yes Use the MULTIDISPENSE mode with V1 set to a slightly higher volume than desired and V2 set to the desired volume The pickup cycle will then aspirate a slight excess of liquid The first press of the trigger in the dispense cycle will dispense the desired volume The second press will dispense the excess to be discarded The third press will initiate the blowout cycle and return the piston to the home position 21 Q 17 Can the EDP pipette be used to measure the volume of liquid in a container A Yes if you know approximately how much liquid is present Place the EDP pipette in the TITRATE mode and set V1 to the approximate volume and V2 to zero Now pick up the liquid in the container If all the liquid was not picked up the volume approxim
7. EDP Electronic Pipette Six pipettes for volume ranges from 0 5 uL to 2 5 mL 100 pL model shown RAININ Foreword CONTENTS For best results read this manual completely before using your EDP Electronic Pipette WARNING This equipment generates and uses radio frequency energy and if not installed and used properly i e in strict accordance with the instructions manual may cause harmful inter ference to radio communications It has been tested and found to comply with the limits for a Class A computing device pursuant to Subpart J of Part 15 of FCC Rules which are designed to provide reasonable protection against such interference when operated in a commercial environment Operation of this equipment in a residential area is likely to cause interference in which case the user at his own expense will be required to take whatever measures may be required to correct the interference N Prices and specifications are subject to change without notice EDP RAININ Certified and Clean Pak are trademarks of Rainin Instrument LLC EDP Pipettes are manufac tured under U S Patent Nos 4 671 123 Other U S and national patents issued and pending Copyright 1984 2003 Rainin Instrument LLC 1 0 2 0 3 0 4 0 5 0 6 0 7 0 8 0 9 0 10 0 11 0 12 0 13 0 14 0 15 0 16 0 17 0 18 0 19 0 20 0 DESCRIPTION 4 enge 2 UNPACKING INSTRUCTIONS 000 0 een 4 INSTALLING PORTABLE POWER PACK
8. HOME DOWN Display and Tone READY PICKUP Pull trigger beep PICKUP Pull trigger DISPENSE DISPENSE wr CUL p BLOWOUT after pause beep y after pause Holding trigger in delays this step beep beep TO SET MODE Press Fi 1 TO SET VOLUME Press OND lE Desired volume TO CLEAR EMPTY OR RETURN HOME C Press F 0 7 QUICK REFERENCE MULTIDISPENSE MODE 2 OPERATION PICKUP DISPENSE DISCARD READY VI REMAINDER BLOWOUT READY PISTON P Di a POSITION TO SET MODE M 2 Press F 2 TO SET NEW VOLUMES V1 Press 1 o ol o E Repeat HOME dl Desired pickup volume i v2 Press 2 1 0 E C i DOWN y Desired dispense volume Pull Pull Pull Pull gt gt gt gt trigger trigger trigger trigger after TO RE ENTER SAME VOLUME ee V1 PRESS E V2 PRESS E Holding trigger in delays TO CLEAR EMPTY this step OR RETURN HOME Press F o Display and Tone beep beep on last beep beep beep beep DISPENSE DISPENSE Ort IL 2071 Z Ol Ve M 8 0 QUICK REFERENCE TITRATE MODE 3 OPERATION PICKUP DISPENSE DISCARD READY VI V2 REMAINDER PISTON er i POSITION
9. P pipette remember volumes A Yes Once a volume setting has been specified in any of the four operating modes that setting will be remembered and used until it is changed by keyboard entries from the user For example An instrument in the PIPETTE mode is set to measure 75 microliters The instrument is then used in the DILUTE mode to mix 90 microliters of diluent with 10 microliters of sample If the instrument is returned to the PIPETTE mode it will be ready to again measure 75 microliters without further keyboard entries When the instrument is next placed in the DILUTE mode it will still be set to aspirate 90 microliters of diluent and 10 microliters of sample This retention of previous volume settings applies to every mode regardless of how many other modes have been used in between as long as power has been applied to the instrument continuously and the encoder plug has not been removed Removing power from the instrument will result in loss of volume settings from memory The instrument will initialize to its default values when power is restored 17 Q 6 What does it mean when the volume indicator on the display is flashing A If the keyboard is enabled symbol KB showing at lower left of display a flashing volume display means a volume entry is in progress or is being called for In modes requiring two volumes the symbol V1 or V2 at the right of the display tells you which volume is being called for The
10. Pack becomes sufficiently low an initialization sequence may occur In this event any stored volume specifications will be lost and must be re entered Q 10 What does it mean if I try to enter a volume and nothing happens A Normally you will know that the EDP drive module is accepting a volume entry because the digital volume indicator begins to flash as soon as the first numeric key is pressed If this does not occur it is probably because the keyboard is locked or the keyboard is not enabled Check the lower left portion of the display for the letters KB and the absence of the word locked See Section 4 for toggling procedures Continued 19 The keyboard lock is for protection When the EDP pipette is used to repeat the same program over a long period of time locking the keyboard eliminates the possibility of unwanted volume or mode changes Q 11 Why should the last aliquot be discarded in the MULTIDISPENSE mode A If V1 is not a perfect multiple of V2 then the final aliquot will be smaller than all the rest Also even if V1 is a perfect multiple the last aliquot will be less accurate than all the others Q 12 How do I know when I have reached the last aliquot in a MULTIDISPENSE sequence A When the next to last aliquot has been dispensed the tone generator will beep and the symbol V2 will disappear from the right side of the display If the last aliquot is smaller than all the others
11. TAND WITH EDP PIPETTE IN PLACE FIGURE 12 1 RAININ CERTIFIED TIP SEAL SURFACE ORIFICE 25 Tips for use with EDP are EDP 100 Maximum Volume 100 uL Bulk Tip Cat No RC 250 RC 250 RC 1000 RC 2500 Rack Tip Cat No RT 250 RT 250 RT 1000 RT 2000 Presterilized Tip Cat No RT 250S RT 250S RT 250S RT 250S RT 1000S RT 2000S EDP 1000 EDP 2500 1000 pL 2500 pL SpaceSaver Cat No GPS 250 GPS 250 GPS 250 GPS 250 GPS 1000 Green Pak Cat No GP 250 StableStak Cat No SS 250 StableRak Cat No SR 250 DODE 26 13 HINTS FOR PROPER PIPETTING EDP Electronic Pipettes are calibrated to deliver accurate volumes under the following conditions 1 The liquid pipetted is water or any liquid with a density not significantly different from a viscosity not significantly greater than and a vapor pressure not significantly greater than that of water 2 The temperature of the room the pipetting instrument and the liquid are all in the range 21 5 C 1 C 3 The pipette is kept vertical during pipetting During aspiration of sample the end of the disposable tip should be immersed 2 to 3 mm below the liquid surface 4 There is no significant adherence of liquid to the surfaces of the disposable tip during pipetting Each tip should be rinsed once before pipetting to assure the best accuracy 5 The pipette tip is touching the wall of the receiving vessel during dispensing The tip should be moved along the w
12. UP HOME or Pull hold Hold trigger trigger trigger Display and Tone beep read volume beep dispensed PICKUP DISPENSE T mm Vi U 1 OOCL A ty KB Pull trigger BLOWOUT after pause Holding trigger in delays this step beep READY oo TO SET MODE T Press F 3 TO SET NEW VOLUMES V1 Press 110110 O E Desired p v2 Press 1115 7 E Desired initial dispense volume TO RE ENTER SAME VOLUME V1 PRESS E V2 PRESS E TO CLEAR EMPTY OR RETURN HOME C Press FIJO beep beep ckup volume 9 QUICK REFERENCE DILUTE MODE 4 OPERATION PISTON POSITION UP HOME DOWN Display and Tone READY PICKUP Pull trigger PICKUP DILUENT VI beep Pull trigger PICKUP ri _ ro PICKUP AIR beep Pull trigger PICKUP SAMPLE V2 beep PICKUP oir OL Vo DISPENSE Pull trigger after pause BLOWOUT after pause Holding trigger in delays this step READY beep beep TO SET MODE D Press F 4 TO SET NEW VOLUMES V1 Press 9 o ONE Desired diluent volume V2 Pre
13. able pipette tips are fairly demanding Tips must seal properly on the EDP shaft and must be flexible and smooth on their inte rior surfaces so that the shaft is not damaged Tips must be free of flash and particulates Microscopic flash at the orifice can limit preci sion and accuracy by causing unwanted retention of liquid by the tip Also the orifice must be the correct size and consistent in size and geometry from tip to tip Both interior and exterior surfaces must be clear smooth and hydrophobic to avoid unwanted retention of liquid as droplets or film which can reduce accuracy and repro ducibility RAININ EDP Electronic Pipettes are designed and cali brated for use with RAININ Certified Disposable Tips Performance to specification by EDP Pipettes is guaran feed only when RAININ Certified Tips are used as recom mended Rainin Instrument LLC accepts no responsibility for damage to EDP Pipettes or for poor liquid measure ment performance resulting from use of non standard pipette tips of inferior quality RAININ Certified Tips are molded from the highest quality virgin polypropylene plastic Only the finest molds and the best possible molding procedures are used Molds are inspected and maintained regularly to assure confor mance with high quality standards Samples from each lot of tips are inspected microscopically to assure that every lot meets these standards Orifice FIGURE 11 2 RAPID CHARGE S
14. all at the end of each dispensing step to remove any excess liquid outside the tip EDP Electronic Pipettes remove many of the operator variables from the pipetting process Inaccuracies due to human error in locating the exact stop positions at the end of the piston stroke or in releasing or pressing the pushbutton too fast or too slow do not exist So long as the above conditions apply most operators will be able to obtain accurate and precise liquid measurements Even when conditions differ from the ideal EDP pipettes can produce good liquid meas urements With liquids much denser than water for example the actual volume measured will be less than the setting on the pipette but consistency from sample to sample will still be excellent If absolute accuracy is important the volume error can be compensated by applying a correction factor to the volume setting Continued 27 28 Liquids of viscosity moderately higher than water such as serum and plasma can be measured accurately by using the reverse mode pipetting strategy to aspirate a larger volume than is dispensed See Section 10 Question 16 By pipetting in reverse mode residual liquid on the interior wall of the disposable tip becomes a less important factor in determining volumetric accuracy Warm and cold liquids can be measured with good accuracy and precision as long as a consistent pipetting rhythm is maintained from sample to sample in order to minimize differenc
15. ation was too small Dispense the liquid back into the container reset V1 to a higher approximate volume and try again If all the liquid was picked up check the tip to see if there is an air segment below the liquid Hold or quickly squeeze and release the trigger until the air has been expelled and the liquid in the tip just reaches the orifice Read the volume indicator and subtract this reading from V1 to get the actual volume of liquid present After taking the reading hold the trigger or press F O to return the liquid in the tip to the container 22 11 CHARGING THE POWER PACK Operating the EDP Electronic Pipette with the Portable Power Pack installed provides maximum freedom of movement and ease of use At the option of the user the EDP pipette may also be operated with the Power Pack removed and the wall transformer connected This has the advantage of providing a slight reduction in weight which may be desirable for long uninterrupted liquid measurement procedures However portability is limited and operating the pipette with the cord attached requires more care and coordination Also any interruption of line current when operating without the Power Pack will cause all stored volumes to be lost from memory The pipette will execute the 8 second initialization sequence when power is restored Any liquid held in the tip at the time power is interrupted will be dispensed during the initialization sequence
16. ccuracy of results as long as both samples and standards are measured with the same instrument EDP Specifications Accuracy Precision Maximum Model Volume pL pL pL s EDP 10 10 1 0 1 0 5 0 04 EDP 25 25 1 0 1 0 3 0 05 EDP 100 100 0 8 0 3 0 2 0 1 EDP 250 250 0 8 0 5 0 15 0 15 EDP 1000 1000 0 8 3 0 13 0 6 EDP 2500 2500 0 8 8 0 12 2 NOTE To determine specification multiply volume setting by the percent figure Compare to the microliter figure The specified limit is the larger value 19 ORDERING INFORMATION All EDP Electronic Pipettes parts and subassemblies may be ordered from Rainin Instrument LLC By phone 8am 8pm EST 800 4 RAININ 800 472 4646 By fax 510 564 1617 or 781 938 1152 7500 Edgewater Drive Oakland CA 94621 Rainin Road Woburn MA 01801 Order online www rainin com The following catalog numbers are discontinued replaced by EDP Plus models OLD NEW CAT NO CAT NO DESCRIPTION Complete Pipettes EDP 10 EP 10 Electronic Pipette 10 uL with Wall Transformer EDP 25 EP 25 Electronic Pipette 25 uL with Wall Transformer EDP 100 EP 100 Electronic Pipette 100 uL with Wall Transformer EDP 250 EP 250 Electronic Pipette 250 uL with Wall Transformer EDP 1000 EP 1000 Electronic Pipette 1000 uL with Wall Transformer EDP 2500 EP 2500 Electronic Pipette 2500 uL with Wall Transformer EP 10ML Electronic Pipette 10 mL with Wall Transformer Ac
17. cessories 6100 080 same Portable Power Pack 6101 049 same EDP Rapid Charge Stand 105 130V 50 60Hz US 6101 048 same EDP Rapid Charge Stand 210 260V 50 60Hz UK 6101 047 same EDP Rapid Charge Stand 210 260V 50 60Hz Euro Continued 37 38 OLD NEW CAT NO CAT NO DESCRIPTION 6100 065 6100 461 6100 066 same 6100 067 same 6100 068 same 6100 069 same 6100 030 same 6100 032 6100 081 6100 462 6100 063 6100 064 UX 6100 044 EX Liquid Ends Liquid End 10 uL Liquid End 25 uL Liquid End 100 uL Liquid End 250 uL Liquid End 1000 uL Liquid End 2500 uL Liquid End 10 mL Replacement Control module Control module for all volumes Replacement Parts Wall Transformer 105 130V 50 60Hz US Wall Transformer 210 260V 50 60Hz UK Wall Transformer 210 260V 50 60Hz Euro 20 WARRANTY All EDP Electronic Pipettes are warranted for one year against defects in materials and workmanship For warranty information regarding the warranty or repair of any EDP instrument please call Rainin Instrument LLC at 800 472 4646 39 RAININ Rainin Instrument LLC 7500 Edgewater Drive Box 2160 Oakland CA 94621 0060 www rainin com PI 17 RI WEB
18. d attached A The encoder plug in the head of the drive module must match the liquid end It is the user s responsibility to verify this Whenever the liquid end is changed the encoder plug must also be changed A good way to check for the correct volume is to observe the LCD display following the 8 second initialization sequence after the encoder plug is inserted The numeric display should show the nominal volume of the liquid end WARNING When changing the encoder plug the keyboard must be unlocked Removing or changing the encoder plug when the keyboard is locked has no effect and could result in measurement errors if a different liquid end has been attached Q 4 What happens if no encoder plug is present A If the keyboard is not locked the numeric display will show and the instrument will not operate If the keyboard is locked the instrument will behave as if the previously pres ent encoder plug were still in place NOTE If the EDP instrument is to be operated at a single volume setting for a large number of tests this feature can be used to ensure that the volume setting can not be accidentally changed Simply set the volume desired Then lock the keyboard remove the encoder plug and store it in a safe place The instrument will operate properly only at the previ ously set volume If the keyboard is unlocked the instrument will not function until the encoder plug is reinserted Q 5 Does the ED
19. e the volumes match Plug the encoder into the receptacle on the head of the drive module As soon as the encoder plug is inserted the EDP pipette should execute the 8 second initialization sequence as if power had just been have been applied NOTE The keyboard must have been unlocked for this to occur FIGURE 14 4 ATTACHING THE LIQUID END At the end of the initialization sequence check the display The instrument should be in the PIPETTE mode and the volume indicator should show the nominal full scale volume of the liquid end you have attached The instrument is now ready for use 15 REMOVING THE TIP EJECTOR Except for the 2500 microliter model each EDP liquid end has an integral tip ejector a movable cylinder surrounding the liquid end and extending up through the retainer ring The tip ejector is operated by pressing the tip ejector button on the head of the drive module In certain applications requiring access to liquids in very deep narrow containers it may be necessary to operate the pipette without the tip ejector To remove the tip ejector proceed initially as if you were changing the liquid end The tip ejector is held in place by the same threaded retainer ring which attaches the liquid end to the drive module Rotate the retainer ring counter clockwise and remove the liquid end from the drive module Then slip the retainer ring and the tip ejector off the shaft Leaving the tip ejector spring
20. es are followed and proper care and mainte nance procedures described earlier in this manual are exercised The EDP pipette will not accept volume settings that are outside the allowable range for each liquid end However it is the responsibility of the user to ensure that the proper liquid end encoder plug is inserted in the control module If the Power Pack does not carry sufficient charge for accurate pipetting the audible tone will warble and the word Lob will appear on the display If the Power Pack is completely discharged the instrument will not operate or emit any sound Recharge the Power Pack See Section 11 before seeking service assistance If anything confusing happens during operation of the EDP pipette this can virtually always be corrected by clearing the pipette To clear the pipette press the key sequence F O The piston will return to the home position and the keyboard will be enabled The one instance where this will not work is covered in Section 10 Question 8 One more general remedy for confusing problems is to remove power or remove the encoder plug from the drive module briefly and then replace it Be sure the keyboard is unlocked if you remove the encoder This will cause the EDP pipette to reset and go through the normal 8 second initialization routine At the end of this routine the instrument will be in the PIPETTE mode and the volume setting will be the nominal full scale volume The
21. es in heating and cooling effects within the pipette A new disposable tip should be used with each sample when measuring liquids with temperatures much different from ambient Maintaining a consistent pipetting rhythm is always a good idea This will assure the best reproducibility from sample to sample In the MULTIDISPENSE and TITRATE modes best results are obtained if all dispensing is performed without delay after aspirating the sample Delays will exaggerate the effects of heat transfer if the temperatures of the sample the instrument and the room are not perfectly matched This will decrease accuracy and precision 14 CHANGING LIQUID ENDS Any of the six available liquid ends can be rapidly mounted on the EDP Drive Module To remove the liquid end first move the EDP pipette to the home position by pressing F o The letters KB should be visible at the lower left on the display If the keyboard has been locked unlock it Now rotate the retainer ring counterclockwise until it is free and pull the liquid end away from the drive module Remove the encoder plug from the head of the drive module and store this with the liquid end Always disassemble each liquid end inspect for salts and residues and clean if necessary before storing for an extended period FIGURE 14 3 REMOVING THE ENCODER PLUG FIGURE 14 1 FIGURE 14 2 ROTATING THE REMOVING THE RETAINING SEAL LIQUID END NOTE When handlin
22. g the encoder plug do not touch the lower surface Skin oil can contami nate the plug leading to poor electrical contact and improper function Should this occur the situation can be corrected by swabbing the lower surface of the encoder plug with isopropanol 29 30 CAUTION When attaching a new liquid end always install the liquid end on the drive module before inserting the encoder plug If the encoder plug is inserted while no liquid end is in place the EDP pipette can not execute a valid initialization cycle To attach first hold the liquid end vertical with its tip resting on a soft surface such as a book or paper towel Move the drive module into place above the liquid end so the actuator pin of the drive module engages the seat on the piston retainer Rotate the liquid end so the tip ejector hole in the shaft is directly below the tip ejector pin Be sure the tip ejector pin passes through the hole Engage the keyway in the liquid end with the key on the drive module Press the drive module down snugly Then thread the retainer ring carefully onto the drive module and tighten NOTE The 2500 microliter liquid end has no tip ejector Just engage the actuator pin thread the liquid end carefully onto the drive module and tighten Store the retainer ring and tip ejector spring for future use with other liquid ends Remove the new encoder plug from the package Check the labels on the plug and the liquid end and be sur
23. in place replace the retaining ring on the liquid end without the tip ejector m Finally reattach the liquid end to the drive module Note that it is not necessary to remove the encoder plug from the drive module when removing the tip ejector Thus any volume specifications in memory will be retained CAUTION Be careful not to press the trigger during this operation FIGURE 15 1 REMOVING THE TIP EJECTOR FROM THE LIQUID END 31 16 CARE AND CLEANING OF LIQUID ENDS For accurate liquid measurement it is important to maintain the integrity of seals between the liquid end and the disposable tip and between the piston and the interior of the liquid end Each pipette should be checked periodically for proper sealing Mount a disposable tip set the instrument to its nominal full scale volume in the PIPETTE mode and aspirate that volume of water Then hold the instrument in the vertical position and observe the tip for 1 2 minutes During that period there should be no loss of liquid from the tip or droplet formation at the orifice For a proper seal between the liquid end and the tip it is important to use only RAININ Certified or Clean Pak disposable tips as recommended in Section 12 of this manual These tips are designed for proper fit with sealing surfaces matching the design of the pipette shaft and proper flexibility to ensure a good seal It is important to avoid nicking or scratching the seali
24. instrument should now function normally Note that this procedure should be applied only as a last resort It has the disadvantage that any existing volume specifications will be lost from the microcomputer memory and must be re entered EDP pipettes are constructed from the highest quality mechanical and electrical compo nents and designed for trouble free operation However in the event that a real electro mechanical malfunction should occur Rainin Instrument LLC maintains a complete serv ice department to correct the problem Please call RAININ for instructions regarding service for your EDP instrument 35 18 PERFORMANCE SPECIFICATIONS Performance specifications for EDP Electronic Pipettes are of two types ACCURACY means the closeness with which the dispensed volume approximates the volume setting of the instrument Accuracy is specified as Mean Error the maximum amount by which the mean value of a large number of replicate measurements of the same volume will deviate from the set volume The specification applies to water in the ambient temperature range of 18 C to 25 C PRECISION refers to the scatter of individual measurements around the mean of a series of measurements It is expressed as standard deviation Specifications for precision are always tighter than for accuracy In most experiments where sample measurements are compared to standards it is the specification for preci sion that will determine the a
25. measurement The movement will stop after about 8 seconds and the instrument will be silent At this time the digital volume display on the LCD panel should become visible Should these initial events fail to occur consult the troubleshooting information in Section 17 of this manual Attach a disposable tip to the liquid end by pressing the shaft into the tip with a firm twist ing motion The EDP pipette is now ready for use NOTE If you have purchased the optional Portable Power Pack accessory the Power Pack may be installed in the EDP drive module and the instrument can then be operated without the power cord attached Before installing the Power Pack it is a good idea to operate the EDP pipette under power from the wall transformer a few times to be sure that everything is functioning properly 3 INSTALLING PORTABLE POWER PACK When you are ready to install the Portable Power Pack first disconnect the transformer cord from the instrument Then remove the cover from the power pack compartment The compartment is located at the rear of the drive module next to the power cord receptacle Plug the cord on the Power Pack into the cord from the pipette which you should have found inside the compartment Be careful to connect the two halves of the plug in the proper orientation The plug is designed so that it can only be connected one way Improper connection could result in damage to either the Power Pack or the EDP pipette When
26. ng surface of the pipette shaft Should the shaft become scratched and fail to produce an air tight seal this may sometimes be corrected by rotating the sealing surface of the shaft inside a small piece of very fine emery cloth Take care during this procedure to remove only the minimum amount of material necessary to restore a proper seal Should this measure fail the shaft should be replaced The seal between the stainless steel piston and the interior of the pipette shaft is formed by a self lubri cating PTFE ring and a fluoroelastomer O ring The PTFE ring extends through the O ring and only the PTFE ring contacts the surface of the piston See Figure 16 1 The O ring actually compresses the PTFE ring against the piston to maintain a proper seal even after moderate seal wear The O ring itself also forms the stationary seal between the PTFE ring and the shaft FIGURE 16 1 TYPICAL PISTON SEAL ASSEMBLY The most important factor in maintaining the integrity of the piston seal is keeping it clean Never allow liquid to enter the shaft where it can come into contact with the piston or the seal The EDP pipette helps you by controlling the rate of upward movement of the piston to avoid splashing In addition whenever liquid is present in the pipette tip the instrument should be held vertically Never invert the instrument or lay it on its side with liquid in the tip Never pull liquid into the shaft by operating the instrumen
27. or wear by sliding it along the piston The seal should offer a slight but definite resistance to movement on the piston The seal should not fall down the piston under its own weight If no resistance is observed the seal should be replaced WARNING Never apply grease to the seal assembly of an EDP pipette The PTFE seal is self lubricating Grease or lubricant residue will alter the effective diameter of the piston and produce errors in liquid measurement Reverse the procedure to reassemble the liquid end Make sure the PTFE seal and O ring are properly positioned on the piston Compress the piston retainer against the piston spring and rotate clock wise to engage the retainer catch Finally replace the liquid end on the drive module Attach a disposable tip and test for proper sealing as described above 2500 MICROLITER LIQUID END The piston assembly is threaded into the shaft Use the seal wrench Cat No 6100 231 to remove and replace the assembly as in Figure 16 3 Reinsertion is most easily done with the shaft inverted After tightening with the wrench push the piston and release to test for free movement If the piston does not return freely the O ring and seal are not properly seated FIGURE 16 3 2500 uL LIQUID END WITH SEAL WRENCH 34 17 TROUBLESHOOTING The EDP Electronic Pipette is very self protective There is actually very little that can go wrong assuming proper pipetting techniqu
28. os oem Iroser sna sous A single volume of liquid is 1 PIPETTE 9 q 2 blank aspirated then dispensed Press FI 1 A single volume of liquid V1 is aspirated then partially M dispensed stepwise as a series Press IFI 2 of equal but smaller volumes V2 2 MULTIDISPENSE A single volume of liquid V1 is aspirated then partially dispensed under user control A smaller volume V2 may be T 3 TITRATE specified to be dispensed Press F 3 1 immediately During dispens ing the display shows the total volume dispensed Two separate volumes V1 V2 of different liquids are aspirated sequentially separated by a 4 DILUTE small air segment Both liquids D are dispensed together Volume Press F 4 V1 the diluent is usually larger than volume V2 the sample 5 QUICK REFERENCE GENERAL KEYBOARD DISABLED PRESS m KEYBOARD ENABLED PICKUP O Ta OE A KEYBOARD LOCKED PICKUP LIL Lit ty KB locked L PRESS F 9 KEYBOARD UNLOCKED PICKUP UU OU Ay AUDIBLE TONES ON PRESS F 8 fi AUDIBLE TONES OFF Keyboard entries other than those shown on this page will be accepted only when the keyboard is enabled and unlocked 2 PRESS PRESS r 6 QUICK REFERENCE PIPETTE MODE 1 OPERATION PISTON POSITION UP
29. r tently set to a very small value especially if the user cannot remember what value was entered for V1 In this case almost any value for V2 will be illegal and the drive module will repeatedly give the error signal for every volume entered If this situation should occur it can best be corrected by entering the smallest possible value for V2 The small est possible value would be 1 1000 of the nominal full scale volume 1 500 for 25 250 and 2500 microliter instruments The display should now return to V1 and permit a legal volume entry sequence to be executed The same difficulty may occur in the DILUTE mode if the value entered for V1 is very close to the nominal full scale volume of the instrument The solution is the same Enter the smallest possible value for V2 to escape then enter the desired legal values for V1 and V2 Q 9 How do I know when the Power Pack needs recharging A The instrument will intermittently warble and display the message Lob on the volume display When this occurs the Power Pack should be recharged by placing the instrument on the Rapid Charge Stand or by attaching the wall transformer If the wall transformer is used operation of the instrument may continue with the cord attached In no case should the instrument continue to be operated without recharging when the low battery signal arises since accurate measurements cannot be guaranteed under these conditions Also if the charge on the Power
30. sory which mounts in a compartment in the drive module The Portable Power Pack can be recharged by connecting the wall transformer The pipette can be operated from the transformer while charging Alternately the EDP pipette with Portable Power Pack installed can be recharged by plac ing it on the Rapid Charge Stand accessory The Rapid Charge Stand provides a conven ient resting place for the pipette when not in use 2 UNPACKING INSTRUCTIONS In addition to this instruction manual the EDP package should contain 1 The EDP Electronic Pipette 2 The wall transformer Carefully remove each part from the package and inspect for shipping damage If any part appears to have been damaged in shipment a damage claim should be filed immedi ately with the shipping carrier Rainin Instrument LLC can assist you in filing a claim Be sure to save the shipping container and all packing materials until the instrument has been checked out and found to function properly To operate the EDP pipette first plug the wall transformer into a convenient power outlet 105 130 volt 50 60Hz or 210 260 volt 50 60Hz matching the specification printed on the transformer Then plug the power cord from the transformer into the receptacle provided on the back of the EDP drive module As power is connected you should feel the drive module begin to purr and move The instrument will execute an initialization routine to properly position the piston for liquid
31. ss 1 0 lo Desired sample volume TO RE ENTER SAME VOLUME V1 PRESS E V2 PRESSE TO CLEAR EMPTY OR RETURN HOME C Press FO 10 QUESTIONS AND ANSWERS Q 1 Is it necessary to enter the decimal point when setting a volume A No If the volume is a whole microliter the decimal point and zeros following it need not be entered In fact with the nominal 1000 and 2500 microliter instruments all possible volume settings are whole microliters and the decimal point is not displayed If the deci mal point is pressed when setting a volume on either of these instruments all following digits will be ignored Q 2 What is the smallest possible increment in a volume setting A For 10 100 and 1000 microliter instruments the smallest possible increment in a volume setting is 1 1000 of the nominal range of the instrument For 25 250 and 2500 microliter instruments the smallest increment is 1 500 of the nominal range With the 25 microliter instrument volume settings are rounded down to the next lower 0 00 or 0 05 microliter With the 250 microliter instrument volume settings are rounded down to next lower 0 0 or 0 5 microliter The 2500 microliter instrument rounds down to the next lower multiple of 5 microliters Q 3 Since all EDP models have the same electronic drive module how does the drive module know the nominal volume of the liquid en
32. t without the proper disposable tip attached Periodically the liquid end should be disassembled for inspection First remove the liquid end from the drive module It is not necessary to remove the encoder plug during this procedure Now depress the spring loaded piston retainer and rotate it counter clockwise a fraction of a turn to disengage the retainer catch Then pull the piston assembly out of the shaft The seal will normally remain attached to the piston If the seal is not attached to the piston invert the shaft and tap it lightly until the seal drops out Inspect the piston the seal and the inside of the shaft for dirt and particulate contamination espe cially salt residues If any part is not perfectly clean remove the contamination by rinsing with a good solvent such as distilled water or methanol The surface of the piston should maintain a high polish as when new If this surface loses its polish or becomes scratched corroded or pitted seal wear will accelerate drastically If any of these conditions exist the piston assembly should be replaced FIGURE 16 2 REMOVING THE PISTON ASSEMBLY FROM THE LIQUID END 33 Always allow the piston assembly and shaft to dry completely before reassembling The piston may be dried with a very soft cloth However paper towels or any other material which may scratch the piston surface should not be used Before reassembling the liquid end check the piston seal f
33. the Power Pack is connected the instrument should purr move and execute the 8 second initialization sequence exactly as it did when power was first applied from the wall transformer If this does not occur but the pipette was operating properly when powered by the wall transformer the Power Pack may have discharged during storage and transit See Section 11 of this manual for recharging instructions Fold the cord so that the plug is positioned neatly on top of the Portable Power Pack Slide the Power Pack into the compartment with the excess cord to the right as shown in Figures 3 1 and 3 2 Once the Power Pack has been installed and fitted snugly in its compartment on the EDP drive module replace the compartment cover FIGURE 3 1 FIGURE 3 2 POSITION PLUG ABOVE POSITION EXCESS CORD POWER PACK TO THE RIGHT 4 LIQUID MEASUREMENT MODES The EDP Electronic Pipette can operate in four distinct liquid measurement modes described in Table 4 1 When power is first applied or after changing the liquid end the EDP pipette defaults to the PIPETTE mode The mode may be changed whenever the keyboard is active The keyboard is active when the symbol KB is visible at the lower left of the display and the word locked is absent If the keyboard is not active see Section 5 To change the mode press the FUNCTION key F followed by the appropriate numeric key as indicated in Table 4 1 Table 4 1 mo
34. volume entry sequence must be completed before the instrument can be used for measurements Q 7 How can an erroneous volume entry be corrected A If the enter key has already been pressed and you are in the PIPETTE mode simply repeat the volume entry with the correct volume If you are in any mode other than the PIPETTE mode press the enter key a second time then repeat the volume entry If the enter key has not yet been pressed when you discover your mistake pressing the sequence Flo clears the erroneous entry and causes the display to flash the previous volume setting The correct volume can now be entered Q 8 What happens if the volume entered is an illegal value A The instrument will emit a warbling sound the volume display will show the message Err for approximately one second and the illegal volume will not be accepted The user must proceed by entering a legal value The following volume entries are illegal 1 Any volume greater than the nominal full range volume 2 Zero or any volume less than the smallest incremental volume Exception In the TITRATE mode Zero is a legal value for V2 3 In the MULTIDISPENSE and TITRATE modes any volume V2 greater than V1 4 In the DILUTE mode any volume V1 or V2 which causes the sum V1 V2 to exceed 101 of the nominal full range volume WARNING In the MULTIDISPENSE and TITRATE modes a possible trap exists if V1 has been inadve
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