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DR 3800

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1. 1 10 mm square cuvette sample cell adapter A 2 1 inch Pour Thru and multi path adapter B 3 1 inch round cell adapter C 3 5 2 Installation of the cuvette sample cell adapters 1 Open the cell compartment 2 Select the correct adapter for the cuvette sample cell type 3 Insert the adapter so the arrow on top of the adapter points to the left Figure 5 on page 16 and the orientation tab fits the groove in the compartment opening The cuvette sample cell type imprint should be legible on the adapter Figure 4 Note The arrow on top of the adapter indicates the direction of the light beam path 15 Installation Figure 5 Installation of a cuvette sample cell adapter Installation 3 5 3 Use of the light shield for measurements The light shield Figure 6 prevents light interference when using 13 mm and 16 mm vial tests and must be in place before measurements can be taken in cell compartment 1 The light shield is required only when using 13 mm or 16 mm vial tests The DR 3800 is shipped with the light shield installed Remove the light shield before using cell compartment 2 The light shield can be stored in the Adapter Box Figure 8 on page 19 Figure 6 Light Shield 17 Installation Installation of the light shield 1 Open the cell compartment 2 Insert the light shield so the arrow on the light shield points to the left and the orientation tab fits the groove in the compartment opening Fig
2. coooccocnncconoconoconocancccononanenannns 9 Sample Blank ii lc 55 Sample LR GE 26 Creada ataca 26 Ee HE 27 Save as User Program ooccocccccccocncoccos 43 54 84 86 Scale UNIES EE 91 93 97 98 SEHR EIST al 33 DEICCE VIEW sitio 92 Sel Check EE 21 send Dala eoni 37 43 54 90 97 Service TIME iii A aae 111 Single Headmg 43 Single Wavelength 24 43 45 59 62 83 84 85 SOMWA niran a a 56 102 SPOZINGAUOMS sear N 7 Standard Addition 43 78 Standard Adjust iii adria 43 47 Stored Dal iia e 36 Stored Programs 24 42 43 44 46 47 48 50 sending Dala rusia dr 43 Stray Light Check munidad 105 System CHECK A ee fe ag 21 111 System Checks 24 101 103 105 106 107 108 A A 112 T TIME CCTV A id 97 98 Time Course 24 38 39 40 41 97 99 100 Time Course Scan Reading ccseccsecseceeeeeeeeenees 98 HMOT ebe 28 43 44 54 83 86 TOUGH S CICCIN aii rias 23 Troubleshooting 121 U Unpacking the instrument oocccoccncccnconocononcnannononos 11 leier 55 102 leie leg KEE 50 User Programs sonido oc 24 57 V VE E S er a Deep 14 Verification ME ais 108 VIEW Table ii as 97 VIS Lamp Lamp Control Camp TISIONY iia dai lia 110 W AA STA ES y haere eee ree tee A ne ee eet 127 WV AV SISTING EID ida 83 86 Wavelength Check cccceceeccseceseceeesceceeeeeeeeeeeeees 103 Wavelength Range cccceccecceceeeceeceeceeeensneeseeees 91 Wav
3. 11 Plug the instrument in CD D EP EB ZA E Ga E CD D A Gz ea D D a gt A GE EE GD GD GD GD Gap GD GD Ga Gap GD GD GD Figure 11 Base of DR 3800 with filter grid 1 Filter grid 3 Filter pad 2 Phillips screw 119 Maintenance 120 Section8 Troubleshooting Problem Display screen Likely Cause Action Attention Measurements with barcode cuvettes Insert the light shield Please insert the light shield generally require the light shield Press OK Absorbance gt 3 5 The measured absorbance exceeds 3 5 IS ie Sm AN pea nino measurement Concentration too high Calculated concentration is higher than Dilute the sample and repeat the 999999 measurement Error Barcode controlnumber Deviation to the stored data Data updating Please update program data Error Program not available Barcode cuvette test missing Please update program data Error The cuvette is soiled or there are Clean the cuvette allow the particles to Clean Cuvette undissolved particles in the cuvette settle Error Selfcheck stopped Please check the lamp Please close the lid Error xx Data updating Check the lamp and replace if Self Check Test stops while starting the Necessary instrument Close the lid Press Start Again Error Selfcheck stopped Self Check Test stops while starting the Please remove the cuvette instrument Please close the lid Remove the cuvette sample cell from the cell compartment
4. Press OK Error Selfcheck stopped Hardware error Error x Contact the manufacturer or a sales Electronic defect representative and indicate the error number Error E Reduce ambient light Avoid direct sun Too much ambient light The instrument sensors detects too light ght Move device into shade much ambient light D Wi or close the lid ose the lid Negative result The calculated result is negative Check the concentration of the sample Check the programming Error in the test database user No evaluation Contact the manufacturer or a sales database representative The measured absorbance is above the Dilute the sample and repeat the Over measuring range ees calibration range of the test measurement If possible select a test with a lower measurement range or use a cuvette with a longer path length The measured absorbance is below the calibration range of the test Under measuring range 121 Troubleshooting 122 Section 9 Replacement Parts Description Tungsten Lamp Cuvette adapter 10 mm A Cuvette adapter 1 inch round C Light shield Cuvette adapter for Pour Thru Cell B Power supply external USB Memory Stick USB Interface Cable 1 m USB Keyboard keyboard layout US USB Barcode Scanner hand held scanner Pour Thru Kit Adapter Box Sample cells glass 1 inch square 10 mL matched pair Sample cells glass 1 cm square matched pair Sample cell glass
5. Wavelength 546 nm Total Drift 0 0010 Abs Initial Reading 0 0000 Abs Final Reading 0 0005 Abs 0 15 min 0 0005 Abs Last 46s 0 0001 Abs 6 8 3 6 Verification kit tic a AAA es p verification Kit 1 2 oO tic a AAA ee p Nominal Yalues 3 Set Number Valid until BVA 50 3 NGos1 NG5 Z NGllys Ho BGeO Cancel Edit OK 108 The last reading and slope for each 15 minute interval are displayed At the end of the hour the overall values are calculated and displayed The test proceeds to completion Press Cancel to return to Optical Checks The Verification Kit see Section 9 on page 123 is designed for periodic monitoring of scattered light photometric accuracy and the wavelength accuracy of the spectrophotometers When results exceed allowable tolerances given in the quality control certificate to the test record contact the manufacturer 0004 19 OCT 2007 gt 2 6 Abs 1 493 Abs 0 591 Abs 0 302 Abs 360 9 nm 807 0 nm Press Verification Kit in the Optical Checks menu Press Nominal Values Press Edit An automatic menu guidance queries values filters wavelength nominal values and tolerances given in the quality control certificate to the following specifications e Stray Light e Photometric accuracy e Wavelength accuracy Press OK when all values are entered and the overview is displayed Advanced Operations 5 Press Verification 6 Insert the ad
6. X Wavelonath To enter the measurement wavelength Use the alphanumeric keypad to enter the measurement 9 wavelength The entered wavelength must be in the range from 320 1100 nm This functions as a stopwatch It helps to ensure that the steps of an analysis are correctly timed e g reaction times wait times etc can be exactly specified When the specified time has elapsed an acoustic signal is emitted The use of the timer has no influence on the measurement program Timer icon Concentration Factor Multiplication factor for converting absorbance values into concentration values Concentration To select the position of the decimal point in the calculated concentration readings Resolution 83 Advanced Operations Single Wavelength WIS Lt Options Concentration Concentration Factor Off Resolution 0 01 Reading Mode Save as Single User Program Trans EE v Instrument Options Return Setup Table 11 Single wavelength setup options continued Single Reading Mode A reading is only displayed after a measurement has been carried out press Read standard setting see section 6 4 2 on page 85 Continuous Reading Mode After the zero measurement all readings are displayed automatically and continuously see section 6 4 3 on page 85 Reading Mode Save as User Program To store the selected parameters as a User Program Call up saved measurement data wavelength scans or time courses see section 5 3 on Rec
7. 1 After performing a procedure clean glass cuvettes sample cells with cleaning agents 2 Afterwards rinse the cuvettes sample cells several times with tap water and then thoroughly with deionized water Important Note Glass cuvettes sample cells that have been used for organic solvents such as chloroform benzene toluene etc must be rinsed with acetone before being treated with cleaning agents In addition another rinse with acetone is necessary as a final treatment step before the cuvettes sample cells are dried 115 Maintenance 7 2 Lamp replacement 1 Remove the cuvette vial from the cell compartment 2 Switch the instrument off 3 Unplug the power cord WARNING Burn Hazard Wait until the lamp cools down Contact with the hot lamp can cause burns 4 Use a screwdriver to remove the cover from the back of the instrument the screws may be slotted or cross head 5 Remove the cover 6 Carefully fold the fan forward see instrument label 1 116 7 8 9 13 14 15 16 17 10 11 12 Maintenance 1 WARNING Burn Hazard Wait until the lamp cools down Contact with the hot lamp can cause burns Push down on the pressure spring see instrument label 2 Remove the halogen lamp complete with the plug panel see instrument label 3 Carefully unplug the halogen lamp from the plug panel Important Note Hold the lamp by the fitting only Avoid touching
8. Fax 204 694 5134 canada hach com HACH LANGE LTD Unit 1 Chestnut Road Western Industrial Estate IRL Dublin 12 Tel 353 0 1 46 02 5 22 Fax 353 0 1 4 50 93 37 info hach lange ie www hach lange ie HACH LANGE SA Motstraat 54 B 2800 Mechelen T l 32 0 15 42 35 00 Fax 32 0 15 41 61 20 info hach lange be www hach lange be HACH LANGE S R L Via Riccione 14 l 20156 Milano Tel 39 02 39 23 14 1 Fax 39 02 39 23 14 39 info hach lange it www hach lange it HACH LANGE S R O Lesansk 2a 1176 CZ 141 00 Praha 4 Tel 420 272 12 45 45 Fax 420 272 12 45 46 info hach lange cz www hach lange cz HACH LANGE 8 Kr Sarafov str BG 1164 Sofia Tel 359 0 2 963 44 54 Fax 359 0 2 866 04 47 info hach lange bg www hach lange bg HACH LANGE E P E 27 Avlidos str GR 115 27 Athens Tel 30 210 7777038 Fax 30 210 7777976 info hach lange gr www hach lange gr Repair Service in Latin America the Caribbean the Far East Indian Subcontinent Africa Europe or the Middle East Hach Company World Headquarters P O Box 389 Loveland Colorado 80539 0389 U S A Tel 001 970 669 3050 Fax 001 970 669 2932 intt hach com DR BRUNO LANGE GES MBH Industriestra e 12 A 3200 Obergrafendorf Tel 43 0 27 47 74 12 Fax 43 0 27 47 42 18 info hach lange at www hach lange at DR LANGE NEDERLAND B V Laan van Westroijen 2a NL 4003 AZ Tiel Tel 31 0 344 63 11 30 Fax 31 0 344 63 11 50
9. The User Programs database is empty when the instrument leaves the factory and is used to accommodate programs created by users specific needs Here are a few examples of entries e Programming of user created procedures The analysis procedure must be developed first before it can be programmed The user must define or determine the program sequences calculation formulas measurement wavelengths factors measuring range limits etc e Modified tests e Assignment of user programs to the favorites menu for frequently used tests e Creation of a specific selection of methods and tests Press User Programs in the Main Menu and then Program Options The Program Options menu contains several input and editing options Table 6 User Programs weng El Program Options Add to EN Favorites Edit Delete Select by Program Cancel Number Options Table 6 Program options user programs G Fe OO Select New to program a new user program Note The first time Program Options is selected only the New option is available The other options remain inactive grey until the first program has been created Add to favorites Select Add to favorites to add an existing user program to the list of frequently used programs COS Select Edit to modify an existing program Select Delete to remove a program from the list of user programs The program will be Delete l simultaneously deleted from the Favorites list 6 1 1 Prog
10. configurations 1 Press Password in the Instrument Setup menu 2 In order to highlight the Security List assign a password Press Set Password 33 Standard Operations Instr ae C ZU 3 New Password Use the alphanumeric keypad to enter a new Password up to MA 10 characters each and press OK to confirm dE abe Apc DEF cut CE The access to the Security List is activated e BER Cancel Inst poet Cte ZEN 4 Password Press Security List to lock various functions for unauthorized users Security CG of C On Instp oae Et mban z 3 Security List 5 Highlight the desired functions to control 6 Confirm the Security List with OK to return to the Password menu Mew Program Edit Program 7 Press On to highlight the new settings of the Security List Delete Program pdate Software 8 Enter the new Password again to confirm Cancel OK 9 Press OK to return to Instrument Setup Note The alphanumeric keypad to the Password inquiry appears when a user tries to reach a locked setting 34 Standard Operations 5 2 7 1 Password deactivation a i Password 1 Press Password in the Instrument Setup 2 Use the alphanumeric keypad to enter the former Password and press OK to confirm Instr eens es Ot y Password 3 Press Off to deactivate the settings of the Security List paa 4 Press OK to return to Instrument Setup Off On Set Password Security List Note Use this
11. limits automatically so that the total range can be displayed 4 Press OK to confirm 5 Press Return to return to the scan mode After the parameters have been selected the instrument must be blanked then the sample can be analyzed 1 Insert the blank cuvette cell into the cuvette sample cell holder Press Zero The blank reading is shown on the display 2 Insert the sample cuvette cell into the cuvette sample cell holder Press Read Start collecting time course kinetic data Note During the measurement the Zero and Read keys change to Mark and Stop Time Course 6 7 3 Analysis of time course data Time Course 2 400 min Options 400 mm Options Advanced Operations e Select Mark to mark the next data point collected This mark is not used by the instrument but is available for the user and may indicate a significant event such as the addition of a sample or other reagent The mark is also shown in the table e Select Stop to stop taking sample readings After the data are collected the following manipulations can be done on the graphic data The Time Course Program is complete if e the sound is turned on the instrument beeps when the readings are done e the graph is shown fullsize e the scaling of the x axis occurs automatically e the Cursor functions in the vertical navigation bar are highlighted 99 Advanced Operations 6 7 3 1 Navigation of Cursor Function Zoom Functi
12. nenu span ee gn Timer Invert Timer 01 00 Shake Timer 00 30 Timer 15 00 General Timer Cancel OK Coo o 10 Al nn a an _ senses wT A Timer mm s5 00 00_ BEE Cancel OK C o 44 Some procedures do not require the use of timers Other procedures require several timers These timers are pre programmed into each Stored Program along with a description of the activity to be performed during the timed period 1 Press the Timer icon on the display 2 Press OK to start the first timer The timer will count down on the screen 3 To start the next timed activity for the Stored Program press the Timer icon and OK Note Press Close to view the measurement screen while the timer is running The time will be shown left side bottom instead of the date Note Press Cancel to stop the timer midway through the countdown Note The Timer will beep when the end of the running time is reached A general purpose timer is also available in many programs When the timer icon is visible press the icon and select General Timer A new screen will appear Enter the length of the timed interval and press OK to start the timer The timer will beep when the timed interval ends Standard Operations 5 4 4 Set the reading mode Optic Reading Mode 1 To highlight the required mode press Reading Mode 2 Selectthe required mode then press OK then Return to return to the result display Ge Single Readi
13. 1 4 Store a user program User Program 950 The input of the basic data is complete An overview of the variable Mame User Program program data is displayed 1 To enter more specifications or change existing ones highlight the appropriate line and press Edit 2 Select Store to save the user program 3 Press the PC Printer icon to send the program data to a printer or to a USB memory stick connect the USB memory stick to the USB interface first Cancel Note The test data will be formatted in the preinstalled PrgData folder as a csv and as a lst file 4 To transfer the program data from one instrument to another create a new folder on the USB memory stick named dbhlc and copy the Ist files into this folder 5 Use the USB memory stick to update the instrument software 6 1 1 5 Additional user defined parameters and functions In addition to the previously defined basic data additional parameters and functions can be defined for user programs e measuring range upper and lower limits e timer functions e chemical forms wg GE a e Upper Limit Upper and lower limits of the measuring range It is possible to enter a maximum upper and minimum lower measurement value An error message is displayed if a reading Is above the upper limit or below the lower limit 6 Highlight the appropriate line in the overview of the program data and press Edit 7 Set On and press the 0 000 key to enter the measur
14. 3 1 Unpack the instrument 3 2 Operating environment WARNING Electrical and Fire Hazards Use only the provided power supply Only qualified personnel should conduct the tasks described in this section of the manual The DR 3800 Spectrophotometer comes packaged with the following items e DR 3800 spectrophotometer e Dust cover e External power supply including 4 adapters for EU UK USA and AUS China e Adapter Box e 3 different cuvette sample cell adapters A B and C e Light shield already installed in the instrument e DR 3800 User Manual e Quick start guide DR 3800 e CD ROM containing the HACH procedures Note If any of these items are missing or damaged contact the manufacturer or a sales representative immediately The following conditions are necessary to ensure correct instrument operation and accurate results e Place the instrument firmly on an even surface Do not push any objects under the instrument e Maintain an ambient temperature of 10 to 40 C 50 to 104 F for proper instrument operation e The relative humidity should be less than 80 moisture should not condense on the instrument e Leave at least a 15 cm 6 in clearance at the top and on all sides for air circulation to avoid overheating of electrical parts e Do not operate or store the instrument in extremely dusty damp or wet locations e Keep the surface of the instrument the cell compartment and all accessories clean and dr
15. 340 nm 1 Press Stray Light Check in the Optical Checks menu wavelength 340 nm 2 Remove any cuvette sample cell from the cell compartment Please remove sample cuvette 3 Insert the adapter A for 10 mm rectangular cells in the cell and touch Zero compartment 2 Press Zero Cancel Zero OPE ARARE a E Stray Light Check 4 Insert the sample cuvette cell or reference filter into the cell compartment 2 Close the cell compartment Press Start Wavelength 340 nm Reading 13 Cancel Start O ti we Lt Lt Stray Light Check 5 The Average and Std Deviation are calculated from O 100 successive absorbance measurements The result is Wavelength 340 nm displayed and is to be compared with the nominal standard NN data given in the quality control certificate of the sample 7 3 0421 Abs cuvette cell Average 3 248 Abs Note Failings and passings will be defined by the user Std Deviation 0 059 Abs Cancel Start 6 Press Cancel to return to Optical Checks 105 Advanced Operations 6 8 3 4 Absorbance check O tic Pa AAA ee P Absorbance Check Wavelength 546 nm Please remove sample cuvette and touch Zero Cancel Zero A O tic xz aale es P Absorbance Check Wavelength 546 nm 0 6130 Abs and touch Read 1 of 10 Cancel Read A O tic mL als p Absorbance Check E Wavelength 546 nm 0 6123 Ext Average 0 612 Ext Std Deviation
16. 50 mm Sample cell with cap plastic 1 cm 10 mL Hach Data Trans PC software for data transfer Certified test filter set for self checks Verification Kit 6 precision glass filters with target values Dust cover Filter pad Citizen PD 24 Thermal Printer 1 roll of paper LINK2SC software SD SanDisk card USB adapter and user manual LZV565 LZV583 LZV584 LZV646 LZV585 LZV610 2946900 5924000 LZV582 LZV566 5940400 LZV743 2495402 2095100 2629250 4864302 LZY274 LZV537 HYHO20 A23766 2960100 LZV774 123 Replacement Parts 124 Section 10 Contact Information HACH Company World Headquarters P O Box 389 Loveland Colorado 80539 0389 U S A Tel 800 227 HACH 800 227 4224 U S A only Fax 970 669 2932 orders hach com www hach com HACH LANGE GMBH Willst tterstrafe 11 D 40549 Dusseldorf Tel 49 0 2 11 52 88 320 Fax 49 0 2 11 52 88 210 info hach lange de www hach lange de DR BRUNO LANGE AG Juchstrasse 1 CH 8604 Hegnau Tel 41 0 44 9 45 66 10 Fax 41 0 44 9 45 66 76 info hach lange ch www hach lange ch HACH LANGE APS Akandevej 21 DK 2700 Br nsh j Tel 45 36 77 29 11 Fax 45 36 77 49 11 info hach lange dk www hach lange dk HACH LANGE LDA Av do Forte n 8 Frac o M P 2790 072 Carnaxide Tel 351 214 253 420 Fax 351 214 253 429 info hach lange pt www hach lange pt HACH LANGE KFT Hegyalja t 7 13 H 1016 Budapest Tel 36 06 1 225 7783 Fa
17. 60 6 1 1 3 Calibration settings for single and multi wavelength mode ooccoocccccccocncccccnonccncnnocononnnonononcnnns 62 6114 Store a user Te e TE EE 66 6 1 1 5 Additional user defined parameters and functions oocccocncccnccncnnncnconncncnnnncnnnnnnnnncnnnnnanonnnonnnnnos 66 6 1 2 Free programming program type ui id 68 6 1 2 1 Measurement ee 68 6 1 2 2 Enter a new element of a measuring Sequence cccocccccncccccccnconnnonnnonnnoncnoncnonanoncnnnnnonaronanonnrnnaninos 69 6 1 2 3 Enter the calibration formula evaluation formula coooccccoccncooncncncnnconnnnnnnnonnnnnncnnnnnnnnnnnnnnnnnnns 71 O E AAA A A O ON 14 6 1 2 5 Save a free programming user program occcocccncncccncccnccocnnonnonnnononononononnnanonnnnonnnnnanonanenanenannannnnns 74 ER WE De IEC a User PO Mi id EE 75 6 1 4 Add edit and delete user programs from the favorites list ooccocccoccccnconnconccnnconncanoconconocanicanons 75 OLA Ee ere el 76 OAZ EE 76 6 og E eG B e epee o O EEE A O II een a a en eee Re ae eee ee 76 Table of Contents 02 AVON Programas ie 17 62221 hecall a TAVOLIC progra israel 17 6 2 2 Delete a favorite program A is 17 6 3 Standard Addition monitoring checking results ooocccoccccnnccocncocnconnconnnnnnnnonnnonnnnnnnonnnnnnnnnnnnnnnnnonnnnnaninaninos 78 6 3 1 Complete a standard addition a a slds 79 6 4 Single Wavelength absorbance concentration and transmittance measurements ccocccnc
18. Operations a Chemical Form 6 1 1 2 Multi wavelength settings User EA Units es Cancel ME 60 4bsorbance Formula Chemical form Enter the chemical formula used in the display to represent the analysis parameter Use the alphanumeric keypad to enter the chemical form and press Next to enter Calibration settings If the Multi Wavelength mode is selected the following parameters can be defined Units Select the required units from the list and press Next Note Units of measure not included in this list can be added in the edit program under Program Options Edit Select Units Edit and then New Absorbance formula multi wavelength program type The Absorbance Formula menu is used to define the wavelengths and the coefficients used in the formula The absorbance formula defines the calculation for the multi wavelength measurement Press the appropriate key to edit the input Press the Formula key In the displayed list select the formula for the program and press OK to confirm wm Absorbance Formula o KiAi1 Kz Az K1M1 Kz2572 K3 452 Kid1 Kz2A2 K343 Kaa Ki 1fKzAz K1A1 KzA72 KzHM3 KiB4 Kz25721 K243 Ka4B4 wm Absorbance Formula Ma K1B1 K 47 K 3A4 Ka4Ba Au Az Az Ma o ZU 600 500 Ki Ka Ka Ka 1 0000 1 0000 1 0000 1 0000 el e Cancel E ae wm z Concentration Resolution Advanced Operations List of available absorbance formulas A is the ab
19. Options Insert the blank cuvette cell into the cuvette sample cell holder Press Zero Note In the reading mode Continuous only the Zero key is shown to start the reading The reading sequence is started automatically Insert the sample cuvette cell into the cuvette sample cell holder Press Options and then the Store icon to store the displayed data in the Data Log Note For data storage see section 5 3 1 on page 36 85 Advanced Operations 6 5 Multi Wavelength mode measurements at more than one wavelength In the multi wavelength mode absorbance values can be measured at up to four wavelengths and the results can be mathematically processed to obtain sums differences and relationships Absorbance measures the amount of light absorbed by the sample in units of Absorbance Transmittance measures the percentage of the original light that passes through the sample and reaches the detector Turning on the concentration factor allows selection of a specific multiplier for converting absorbance readings to concentration In a graph of concentration versus the absorbance the concentration factor is the slope of the line Concentration is calculated using a single factor for each wavelength which is input by the user 6 5 1 Set the reading mode at different wavelengths Press Multi Wavelength in the Main Menu Press Options for Parameter Setup Multi Wavelength VIS Options Concentration Concentratio
20. be compared with the nominal standard data given in the quality control certificate of Wavelength 807 nm the sample cuvette cell Peak 807 0 nm 4 Press Cancel to return to Optical Checks Cancel Start 6 8 3 2 Noise check The Noise Check test is used to test the photometric noise in the instrument However this test can be used to test noise at any wavelength and at an absorbance level determined by using a sample 1 Press Noise Check in the Optical Checks menu 2 Press A to input the wavelength 3 Input the wavelength and press OK to confirm Cancel Zero x 103 Advanced Operations Optic CSL A AS Noise Check 4 Press Zero Wavelength 546 nm eroing Cancel 5 Insert sample cuvette cell into the cell compartment 2 Wavelength 546 nm 6 Press Start Reading Cancel MN noise Check 7 The result will be displayed Thirty readings are averaged for E the blank The Average and Std Deviation are calculated Wavelength 546 nm from 100 consecutive absorbance readings Noise 0 6137 Abs Average 0 6156 Abs e Noise Std Deviation 0 0001 Abs e Sample Sample 131 46 nA Oo Average 131 49 n Reference 8 Press PC amp Printer icon to send data to a PC or Printer 9 Press Cancel to return to Optical Checks 104 Advanced Operations 6 8 3 3 Stray light check The Stray Light test is used to measure the stray light in the instrument at
21. be displayed Units Choice of absorbance or transmittance Send Data To send Data to a printer computer or USB memory stick Type A Call up saved measurement data wavelength scans or time courses see section 5 3 on Recall Data page 36 Instrument Setup Basic data of the instrument see section 5 2 on page 25 97 Advanced Operations Ti me HA e woo A Time amp Interval Total Time 5 Minutes Reading Interwal 12 seconds Cancel OK Pr EE Optic Scale amp Units Limits CG Abs Scale CG Auto C Manual 0 300 Abs nu 3 500 Abs Cancel OK A 6 7 2 Time course scan reading Time Course VIS 400 nm Options 98 Time interval 1 Press Time amp Interval in the Options menu 2 Input the total time and the reading time and press OK to confirm Note In total 500 measuring steps are possible To select a total time and a time interval that would cause this number of measurements to be exceeded the time interval is defined automatically and the OK key is inactivated Scale 8 units 1 Press Scale amp Units in the Options menu 2 Highlight Abs or T as the required units 3 Highlight Auto or Manual scaling on the graph s y axis Note If manual scaling is selected use the alphanumeric keypad to set the limits Y min and Y max The graph is adjusted to display only the values in the selected range If automatic scaling is selected the instrument sets the
22. display Note If undiluted samples are used set the dilution factor off Chemical Form Some of the stored tests methods allow to select the chemical form and the measuring range Edit To modify an existing program Save as User Program as User Save as User Program To store the selected parameters as a User Program Dilution Factor Off On Call up saved measurement data wavelength scans or time courses see section 5 3 on Recall Data page 36 Instrument Setup Instrument Setup Basic data of the instrument see section 5 2 on page 25 54 5 5 5 Sample blank 302 Ammonium After blank value corr 117 l gt EP 2006 14 10 45 Ah Options 5 5 6 Update of a barcode test Main tes Barcode controlnumber Please update program data Standard Operations Turbidity and color in the sample matrix can falsify the results of a photometric analysis The interference factors come from the sample or are created by reactions with the reagents The influence of turbidity and or color can be eliminated or reduced by taking a sample blank reading In the barcode mode a special cuvette vial TNT 919 containing the sample blank is placed in cell compartment 1 after the sample reading has been taken and is automatically measured The sample reading is then corrected by adding or subtracting the blank value The final result is displayed with the message After blank value corr Some barcode tests do not require a
23. function to delete the former Password or to enter a new one 5 2 8 Select color Select one of the four preset color palettes in the Select Color menu we i Select Color 1 Press Select color in the Instrument Setup A color chart list will appear background E 2 Select a color category to highlight the color for the display 3 Press OK to return to Instrument Setup r 5 cancel ok 35 Standard Operations 5 3 Store recall send and delete data 5 3 1 The data log 5 3 1 1 Auto manual data storage 10 Aluminum Alumin 13 SEP 2006 14 49 13 Abs W Trans ww Options The data log will store up to 1000 readings taken in the modes Stored Programs Barcode Programs User Programs Favorite Programs Single Wavelength and Multi Wavelength A complete record of the analysis is stored including the Date Time Results Sample ID and Operator ID The data storage parameter indicates whether data are to be stored automatically or manually in which case the user has to decide which data to store 1 Press Store On Off in the Options menu e With the Store On setting all measurement data are stored automatically e With the Store Off setting no measurement data are stored However this setting can be changed to Store On in the result display through configuration The reading currently shown in the display is then stored Note When the instrument s memory data log is full the oldes
24. info hach lange nl www hach lange nl HACH LANGE S L U Edif Arteaga Centrum C Larrauri 1C 2 PI E 48160 Derio Vizcaya Tel 34 94 657 33 88 Fax 34 94 657 33 97 info hach lange es www hach lange es HACH LANGE S R O Ro n cka 21 SK 831 07 Bratislava Vajnory Tel 421 0 2 4820 9091 Fax 421 0 2 4820 9093 info hach lange sk www hach lange sk HACH LANGE SU ANAL Z S STEMLER LTD T Hilal Mah 75 Sokak Arman Plaza No 9 A TR 06550 ankaya ANKARA Tel 90 0 312 440 98 98 Fax 90 0 312 442 11 01 bilgi hach lange com tr www hach lange com tr 125 Contact Information 126 Section 11 Limited Warranty Hach Company warrants its products to the original purchaser against any defects that are due to faulty material or workmanship for a period of one year from date of shipment unless otherwise noted in the product manual In the event that a defect is discovered during the warranty period Hach Company agrees that at its option it will repair or replace the defective product or refund the purchase price excluding original shipping and handling charges Any product repaired or replaced under this warranty will be warranted only for the remainder of the original product warranty period This warranty does not apply to consumable products such as chemical reagents or consumable components of a product such as but not limited to lamps and tubing Contact Hach Company or your distributor to initiate
25. linear curve corresponds to the standard setting Press Next Curve to display the polynomial 2nd order curve Press Next Curve again to display the polynomial 3rd order curve Advanced Operations 9 Press Force 0 to change the setting from Off to On The curve then passes through the origin of the coordinate system Note This may have an adverse effect on the correlation coefficient r2 10 Press Table to display the table again 11 When the table has been completed and the curve type has been chosen press Done when the graph is displayed or Exit when the table is displayed Go to section 6 1 1 4 on page 66 Calibration by entering the formula wee 4 Calibration Press Enter Formula and press Next Enter Yalues Read Standards Enter Formula ce Cancel User nen Enter Formula 2 Press the formula key A list of available formulas linear and 2nd and 3rd order polynomial is displayed Up to 4 coefficients can be entered S z depending on the selected formula Press the required formula 0 0000 5 0000 0 0000 3 Depending on the selected formula the required coefficients a b C are displayed Press the coefficient keys and enter the corresponding values via the alphanumeric keypad After es Cancel each entry press OK to confirm Note The coefficients can have 5 digits and can have a positive or a negative sign C a b cA 65 Advanced Operations 6 1
26. safety procedures and the correct use of chemicals and to carefully read all relevant Material Safety Data Sheets Normal operation of this instrument may involve the use of hazardous chemicals or biologically harmful samples e The user must observe all cautionary information printed on the original solution containers and safety data sheet prior to their use e All waste solutions must be disposed in accordance with local and national law e The type of protective equipment must be selected according to the concentration and amount of the dangerous substance at the specific workplace The DR 3800 Spectrophotometer is a VIS spectrophotometer with a wavelength range of 320 to 1100 nm The instrument comes with a complete set of application programs and multi language support The DR 3800 Spectrophotometer contains the following application modes Stored Programs pre installed tests Barcode Programs User Programs Favorite Programs Single Wavelength Mode Multi Wavelength Mode Wavelength Scan and Time Course Mode The DR 3800 Spectrophotometer provides digital readouts in direct concentration units absorbance or percent transmittance When a user generated or programmed method is selected the menus and prompts direct the user through the test This menu system can also be used to generate reports statistical evaluations of generated calibration curves and to report instrument diagnostic checks Section 3 Installation
27. the Producer for disposal at no charge to the user Note For return for recycling please contact the equipment producer or supplier for instructions on how to return end of life equipment producer supplied electrical accessories and all auxiliary items for proper disposal This symbol indicates that the instrument contains a Class 1 LASER device Data 0 3 mW 650 nm General Information 2 1 3 Class 1 LASER A Class 1 LASER is installed in this instrument Class 1 LASERS are products where the radiant power of the LASER beam accessible the accessible emission is always below the Maximum Permissible Exposure value Therefore for Class 1 LASERS the output power is below the level at which it is believed eye damage will occur Exposure to the beam of a Class 1 LASER will not result in eye injury Class 1 LASERS may therefore be considered safe However Class 1 LASER products may contain LASER systems of a higher Class but there are adequate engineering control measures to ensure that access to the beam is not reasonably likely Examples of such products include LASER printers and compact disc players CDRH assession number 0510555 02 Data 0 3 mW wavelength 650 nm 2 1 4 Chemical and Biological Safety 2 2 Overview of product 10 DANGER Potential Chemical Biological Exposure Hazards Handling chemical samples standards and reagents can be dangerous Users of this product are advised to familiarize themselves with
28. to Favorites and press OK to confirm The program can now be selected from Favorite Programs menu in the Main Menu 9 9 Barcode Programs Standard Operations A special barcode reader in cell compartment 1 automatically reads the barcode on the 13 mm cuvette vial as the cuvette vial completes a single rotation The instrument uses the barcode identification to automatically set the correct wavelength for the analysis and calculates the result immediately with the help of the stored factors In addition measured values are recorded at 10 different positions during a rotation A special outlier elimination program is run and then the average of the measured values is calculated Cuvette vial errors and soiling are recognized and highly precise results are obtained Figure 10 Cell compartment 1 for barcode 13 mm 16 mm cuvettes vials 51 Standard Operations 5 5 1 Complete a barcode 13 mm test vial Main Menu O Stored HI Barcode Programs Programs User Favorite Programs Programs Reading barcode Please wait Wavelength Scan System Instrument Checks Setup Barcode Programs VISE 320 nm Please insert barcode cuvette 21 SEP 2006 14 01 00 2 59 ee MAaT 200F 12 56 23 il Options 52 1 Insert the light shield in cell compartment 2 2 Prepare the barcode 13 mm cuvette vial in accordance with the working procedure and insert the cuvette vial in cell compartment 1 e When a coded cuvette sam
29. warranty support Products may not be returned without authorization from Hach Company Limitations This warranty does not cover e Damage caused by acts of God natural disaster labor unrest acts of war declared or undeclared terrorism civil strife or acts of any governmental jurisdiction e Damage caused by misuse neglect accident or improper application or installation e Damage caused by any repair or attempted repair not authorized by Hach Company e Any product not used in accordance with the instructions furnished by Hach Company e Freight charges to return merchandise to Hach Company e Freight charges on expedited or express shipment of warranted parts or product e Travel fees associated with on site warranty repair This warranty contains the sole express warranty made by Hach Company in connection with its products All implied warranties including without limitation the warranties of merchantability and fitness for a particular purpose are expressly disclaimed Some states within the United States do not allow the disclaimer of implied warranties and if this is true in your state the above limitation may not apply to you This warranty gives you specific rights and you may also have other rights that vary from state to state This warranty constitutes the final complete and exclusive statement of warranty terms and no person is authorized to make any other warranties or representations on behalf of Hach Company Limi
30. 0 press Instrument Update in the System Checks menu 5 Connect the USB stick to the USB interface on the DR 3800 section 3 4 on page 13 Press OK The link is established automatically and the software is updated 6 Press OK to return to the System Checks menu Note When the instrument software has been updated a prompt to restart the instrument is displayed For each optical check the measured results must be evaluated against user specific requirements The check options do not define tolerances 1 Press Optical Checks in the System Checks menu The Optical Checks menu contains programs for checking the wavelength accuracy stray light and photometric accuracy An optional test filter set Verification Kit Section 9 on page 123 containing 6 precision glass filters target values tolerances and instructions is available and is recommended as an aid for carrying out comprehensive in house instrument checks Advanced Operations 6 8 3 1 Wavelength check The Wavelength Check test is used to check wavelength accuracy at 807 nm 1 Press Wavelength Check in the Optical Checks menu Wavelength 807 nm 2 Insert the adapter A for 10 mm rectangular cells in the cell compartment 2 and insert the sample cuvette cell Neodym or Please insert sample cuvette BG20 2 in the adapter Close the cell compartment and touch Start Press Start Cancel Start P Wavelength Check 3 The result is displayed and is to
31. 0 000 Ext Cancel Zero Zu The Absorbance Check test is used to test the photometric accuracy and repeatability of the instrument This test can be used to test absorbance at any wavelength by a specific sample or test filter set Refer to section 6 8 3 6 on page 108 1 Press Absorbance Check in the Optical Checks menu 2 Press A to input the wavelength 3 Input the wavelength and press OK to confirm 4 Remove any cuvette sample cell from the cell compartment and press Zero 5 Insert sample cuvette cell into the cell compartment and press Read 6 10 Replicates of blanking and reading lead to results 7 The result is displayed and is to be compared with the nominal data of the sample cuvette cell 8 Press Cancel to return to Optical Checks 106 Advanced Operations 6 8 3 5 Drift check The Drift Check test is used to test the stability of the instrument Note The Drift Check runs 1 hour 1 Press Drift Check in the Optical Checks menu 2 Press A to input the wavelength 3 Input the wavelength and press OK to confirm Cancel 4 Press Start Cancel 5 The Drift Check runs 1 hour Wavelength 546 nm This test takes a reading every minute for one hour Every fifteen minutes linear regression is used to calculate the slope 0 0002 Abs rate of change for the previous fifteen minute interval 59 56 Cancel 107 Optic gt CSL A AS Advanced Operations Drift Check
32. 22 Section5 Standard Operations 5 1 Overview 5 1 1 Tips for the use of the touch screen The entire screen is touch activated To make a selection press the screen with a fingernail fingertip pencil eraser or a stylus Do not press the screen with a sharp object such as the tip of a ball point pen e Do not place anything on top of the screen to prevent damage or scratching on the screen e Press keys words or icons to select them e Use scroll bars to move up and down long lists very quickly Press and hold the scroll bar then move up or down to move through the list e Highlight an item from a list by pressing it once When the item has been successfully selected it will be displayed as reversed text light text on a dark background 5 1 2 Use of the alphanumeric keypad Instr San en Cte a Sample ID cecon 123 STU YWX YZ_ Cancel e geff This display is used to enter letters numbers and symbols as needed when programming the instrument Unavailable options are disabled grayed out The icons on the right and left of the screen are described in Table 1 The central keypad changes to reflect the chosen entry mode Press a key repeatedly until the desired character appears on the screen space can be entered by using the underscore on the YZ_ key Note A USB keyboard with US keyboard layout or a USB Barcode handset scanner can be used for input see Section 9 on page 123 Table 1 Al
33. ES O y 64 Calibration by reading standards Press Read Standards and press Next To enter the standard concentrations in the displayed table press the symbol Use the alphanumeric keypad to enter the standard concentration Press OK Press the symbol again see arrow and enter the next standard concentration Repeat this sequence until all standard conentrations maximum of 24 solutions have been entered Highlight the line with the appropriate concentration and insert the cuvette cell with the corresponding standard solution Insert the zero solution into the cell compartment Press Zero Insert the first standard solution into the cell compartment Press Read Insert the second standard solution into the cell compartment Press Read Repeat this sequence until all the standard solutions have been measured maximum of 24 solutions The entered and measured data are displayed in the table Note To delete a standard concentration highlight the appropriate line and press the Delete icon The timer icon shown in the display helps to ensure when necessary that the steps of an analysis are correctly timed e g reaction times wait times etc can be exactly specified When the specified time has elapsed an acoustic signal is emitted The use of the timer has no influence on the measurement program 7 When the data have all been entered and the measurements have all been completed press Graph The
34. H of the sample in the correct range g Is the pipette volume correct 2 Check the used reagents by repeating the standard addition procedure with freshly prepared reagents Detection rate Conclusion The originally used reagents were defective Check the standard solution Repeat the standard addition procedure with a freshly prepared standard solution Detection rate Conclusion The originally used standard solution was defective If none of these measures resolves the problem please contact the manufacturer or a sales representative 6 3 1 Complete a standard addition Carry out the standard addition in accordance with the corresponding procedure There are two different methods Peak volume Standard addition Defined volumes of a standard solution are added step by step to an already analyzed sample The sample is measured again after each addition Sample volume A defined volume of a standard solution of known concentration is added to the volume of sample specified in the procedure and the sample is measured after each addition In most cases three different standard solutions are prepared and the procedure is repeated for each of them Note The units and chemical forms used for the sample are used for the standard solutions Be careful to ensure that to use the correct units for subsequent entries 79 Advanced Operations 10 Aluminum Alumin Peak volume sample volume methods 1 Select Store
35. Instrument Setup or if an Operator ID is already assigned select the Operator ID symbol immediately in the results screen Use this option to enter up to 100 Sample Identification tags up to 13 characters each into the instrument Sample IDs can be used to specify the sample location or other sample specific information 1 Press Sample ID in the Instrument Setup 2 Press New to enter a new Sample ID 3 Use the alphanumeric keypad to enter a new Sample ID Note If a USB Barcode handset scanner see Section 9 on page 123 is connected Sample IDs can also be scanned Sample IDs can also be entered with a USB keyboard 4 Press OK to confirm Instr een AER Ot Sample ID el Number Neu Delete OK 5 2 3 Date and time Instr am SA a 8 Date amp Time Date Em SEP 2006 Time 24Hour Cancel e Standard Operations 5 To number the Sample IDs sequentially e g Inflow 01 etc select Add Number e Use the arrow keys to specify the first number of the sequence e Use the key between the arrow keys to enter the first number of the sequence using the alphanumeric keypad 6 Press OK to return to Instrument Setup 7 The Sample ID is activated Each Sample ID is automatically numbered in ascending order after a measurement The number is shown in parentheses behind the Sample ID Note To remove a Sample ID highlight the ID and press Delete Note A Sample ID can be entered or change
36. LD DOC022 53 00782 DR 3800 USER MANUAL January 2009 Edition 1 Hach Company 2009 All rights reserved Printed in Germany ph sk Table of contents Section 1 PeECciMICACON CN T DECTION 2 E EC HR te MAN ed 9 E EE t te DEE 9 Zi Use or nazald Mile rei en E 9 2 1 2 P PECAUMIONIANY e 9 ZAS Glass LASER tri dades is 10 2 1 4 GRemical and Biological Safety Tod 10 2 2 ONES WO ele ele e a ici 10 SECTION SIMS UE ue BEE 11 1 WMPACK AMS MASIA A E 11 9 2 Operating CNVIFOMIMEN EE 11 3 9 OWE COMM CCUOMS xis tds 12 FOLIC CUO ias 13 3 5 Cell compartments cuvette sample cell adapters light shield and Adapter BOX ooccooccoccccnccocccocncoccocnconnnanos 14 3 5 1 Cell compartments and adapters oi ici 14 3 5 2 Installation of the cuvette sample cell adapters cooocccocccocncccnncocococnononononnnncncnnnonnnnnnronnnnoncnnnnnnnnnninoos 15 3 5 3 Use of the light shield for measurements occoccnccocccccncnccncncncconoconononoconnonnnonnnonnnnonnnonnnnnnnonanenannnannenanenono 17 os A a A 19 o BOAN P AIT A Ee 20 Seccion SAn UP ca late 21 4 1 Power the instrument on and off is 21 AZ AMO UA EN e a A a E E 21 A lO NEC a aii lala 21 Section 5 Standard Operations iii io 23 e OV CIVIC aieiaa a 23 5 1 1 Tips for the use of the touch SCA eege eege EE Edge 23 5 1 2 Use Of the alphanumene keypad EE 23 FA MAN NEN era a r S EA 24 AS A 25 BS A A O 25 SEAS le EE 26 de Daiana UMSS daba 27 9 2 4 Display and sound Preferences o a a
37. T P PO P total P PO total P20 P20 total Save as i Cancel Default p A K s 7 The chemical form of the test result of a number of parameters can be selected individually In the result display press the unit e g mg L or the chemical representation of the evaluation form e g PO P A list of possible evaluation forms is displayed from which the required form can be selected Press OK to confirm Another way of changing the standard setting is 1 In the result display press Options gt More gt Chemical Form A list of available evaluation forms appears 2 Select the required chemical form and press OK to confirm Note The selected chemical form is displayed but does not become the default To change the default see section 5 5 3 1 5 5 3 1 Change of the default setting of the chemical form 1 Insert the blank or sample cuvette vial depending on the working procedure into the cell compartment 2 Inthe result display press Options gt More gt Chemical Form 3 A list of available evaluation forms appear Select the new default setting 4 Press Save as Default The current result and all further measurements will be displayed in the new chemical form 53 Standard Operations 5 5 4 Basic test specific and sample specific data settings Press Options to change test or sample specific settings 349 Phosphate WIS Lt Options CH Send Reading Mode l Data Single iets i Diluti
38. a A a i 99 6 7 3 1 Navigation of a time scan or a time scan analysis oocccocncconccocncocnccononononnnnnnnnnnnnnnnnnnonnnonnnnnos 100 MS o A 101 6 6 1 uge Et ug alte lg ut le EE 101 6 8 2 Upgrade of the instrument software oocccoccccccoccnoconoconoconnnonconononononnnnnnnnnnnnonanonnonannnannnnnenannnaninnnnnss 102 6 9 3 OPUCal CHOC ut e ici de del add 102 630 351 Wavelength EE 103 o aie cceln dsc crete es ete eco O ae aoc aeaeeten 103 AE Silay MOM CNSC ts 105 a A A a I 106 o Kee ee eet Ee 107 0 80 00 NEEN Mesias ee 108 6 6 A E EA 110 OOS LaMO NISTO deene EE en 110 6 0 01 1 Acto Ne 110 0 30 SONICOUN aia bes 111 6 97 Il ge le E EE 112 SECTION 7 MalntenancCe ii E co DA eee ae etorri 115 TA CICANING TE QUIENES rai 115 en EN Specropnolometer EE 115 AAA AA eee ee ere er eee ree eee ae 115 Pleo TEE une e 115 1 2 Lamp ACME ica 116 TS Fiter e ab a E Le 118 ESA Filter Dad A ege Ee GE EE 118 DECTION S Troubleshooting e ee 121 Section 9 Replacement AUS cia daa liada 123 Section 10 Contact information seisseen ira 125 Section TT Limited MECHER ii de id 127 La Le lt gt EE 129 Table of Contents Section1 Specifications Specifications are subject to change without notice Performance specifications Operating Mode Transmittance Absorbance and Concentration Source Lamp Gas filled Tungsten visible Wavelength Range 320 1100 nm Wavelength Accuracy 1 5 nm wavelength range 340 900 nm Wavelen
39. aa 28 DZ DILAIND COMOL EE 29 320 PC and A a casted 30 2051 PANE SEUD rd la 30 320 A eee en eee eee 32 520 9 PACH Data RTE 32 92 Oe el E e rr A E ee ee ee e dee ad e 33 PAS WN OL CU iiss a a a a A 33 O62 Li ASS lee e Le EE 35 OCIS CU OOM ee ee 35 Table of Contents 5 3 Store recall send and aelete dala ali at a 36 So 8 Ka E We e BEE 36 302k 1 AUTO MANU Re Ee EE 36 5 3 1 2 Recall stored data from the data log occcocccoccoccncocncconccocnonononononanonnnnonanonanonanonannonnnonnnnnnanos 36 So Send data Tom NG Gala lOO a ata 37 5 3 1 4 Delete stored data from the data log oocccoccccccoocncconccnonononoconononnonanonanonononnnnonanonanonannenanos 38 5 3 2 Store recall send and delete data from wavelength scan and time course ccoocccccccccccccnccncnnncnnnoncnanonons 38 5 3 2 1 Data storage from wavelength scan or time course occoocccncncnccnnccncncnnncnnnononnnnnnnnnnnnonanonanonanonnnoss 38 5 3 2 2 Recall stored data from wavelength scan or time course oocccocncocncocncoccnoncnnncnnnnnnnnnonanonaconannnnonos 39 5 3 2 3 Send data from wavelength scan or time course ooccoccccncccccncocncnnnncncnnonncnnnonnnnnnnnonononanonnnnoncnnnnnns 40 5 3 2 4 Delete stored data from wavelength scan or time course oooccccocnccccccocncocnconnnnnnnnnnnnnnnnnnnnnnnnnnnnnos 41 349 OCA EIERE EE 42 5 4 1 Select a saved test method entering user specific basic data 42 9 4 2 Stored EIERE Ee 42 O43 SE Of pr
40. aes GERGEN 1 Press the Store icon in the Options menu after a reading is taken Reference of 400 A 500 nm Integral 279 00 Main Menu Options Wavelength Scan The Store Data list will be displayed 15 SEP 06 14 20 33 Scan 7 400 600 nm amp 001 nm i 2 Press Store to save the current scan to the highlighted 16 SEP 06 09 19 40 Scan 6 400 600 nm A 001 nm numbered line A scan can also be overwritten 16 SEP 06 09 26 44 Scan 4 400 600 nm 4 001 nm 16 SEP 06 09 29 14 Scan 10 400 600 nm 4 001 nm Scan 11 Cancel as 38 Standard Operations 5 3 2 2 Recall stored data from wavelength scan or time course Wavelength Scan 14 20 33 400 600 nm 4 001 nm 09 19 40 400 600 nm 4 001 nm 00 26 44 400 600 nm 4 001 nm 09 29 14 scan 10 400 600 nm 001 nm 18 SEP 06 10 01 32 Scan 11 400 600 nm 4 001 nm al an Table Graph Menu Options Wavelength Scan 1 599 Abs 400 A 500 nm Integral 219 00 Main EA Menu summary Options Wavelength Scan nm Abs Min Max nm Abhe Min Max 0 494 401 0 504 403 Peak 0 500 405 0 453 Valley 407 0 475 409 0 481 411 Integral 279 00 MEWN aih Summary Options 1 Press Recall Data in the Main Menu a Select Wavelength Scan or Time Course to recall data b Ifa program is already in progress press Options gt More gt Recall Data 2 Press Graph to look at details Note Press View Summary to return to the Rec
41. all Data page 36 Instrument Setup Basic data of the instrument see section 5 2 on page 25 Concentration Factor Concentration factor 1 Press Concentration Factor Off in the Options menu Press On to highlight this feature 2 Press the Factor key and use the alphanumeric keypad to enter the factor by which absorbance readings are to be multiplied Press the Unit key to select the units for concentration measurements or to create a new unit 3 Press OK to confirm Optic Concentration Resolution Concentration resolution ca 1 Press Concentration Resolution in the Options menu C 0 1 2 Select the resolution and press OK to confirm amp 0 01 C 0 001 Cancel OK 84 GG Single Reading Continuous Readings 320 nm 1 0 921 s 2 25 SEP 2006 09 14 24 3 h Options 1 Advanced Operations Reading mode To highlight the required mode start by pressing Reading mode Select the required mode then press OK then Return to return to the result display Insert the blank cuvette cell into the cuvette sample cell holder Press Zero Note The Read key is only active after the zero measurement has been completed Insert the sample cuvette cell into the cuvette sample cell holder Press Read For data storage see section 5 3 1 on page 36 6 4 3 Take single wavelength measurements continuous readings Single Wavelength EROS 4 0 916 foe EEE 09 15 28 el
42. all Data list 3 Press Table to look at details Note Press View Summary to return to the Recall Data list 39 Standard Operations 5 3 2 3 Send data from wavelength scan or time course 15 SEP 06 Scan 16 SEP 06 scan 16 SEP 06 Scan 4 18 SEP 06 Scan 10 18 SEP 06 Scan 11 IA 400 600 nm 4 001 nm 09 19 40 400 600 nm 4 001 nm 00 26 44 400 600 nm 4 001 mm 09 29 14 400 600 nm A 001 nm 10 01 32 400 600 nm 4 001 nm W Graph W Table Options Cursor Mode CH Track Integral S Return 40 Wavelength Scan Send Data Instrument Setup Option 1 Press Recall Data in the Main Menu and then Wavelength Scan or Time Course 2 Press Options and then the PC amp Printer icon to send the data to a USB memory stick to a printer or to a PC with Hach Data Trans e When a printer is connected select how to send the data to the printer graph table or both graph and table e When a USB memory stick is connected the files will be automatically sent as CSV files Comma Separated Value to a file WLData Wavelength Scan Data or TCData Time Course Data to the USB memory stick The file name will be formatted as ScanData_X csv Wavelength Scan Data or TCData_X csv Time Course Data X number of scans 1 20 For further processing use a spreadsheet program Note The advice Data already exist Overwrite appears when the files were al
43. and reference scan is indicated highlighted at each position of the cursor Note A black and an orange box are shown in the left corner of the display The orange box relates to the reference scan and the black one relates to the current wavelength scan Press the black or orange small box in the left upper corner on the screen to switch between the actual wavelength scan and reference scan Advanced Operations 6 7 Time course of absorbance transmittance The Time Course Mode is used to collect data in either absorbance or transmittance for a user specified length of time After the data are collected they can be displayed in either graphic or tabular format 6 7 1 Time course setup parameters Press Time Course mode in the Main Menu Press Options to configure parameters Time Course VIS Xi Options E Send Time amp Interval View 25 SEP 2006 10 18 03 Table h Instrument Options Return Setup Table 15 Time course setup options For further Options f To input the total time for data collection and the time interval between the collection of data Time amp Interval points To input the wavelength setting To display readings in absorbance transmittance or concentration This can be changed after View Table sample data are collected Scale In the automatic scaling mode the y axis is automatically adjusted so that the total scan is displayed ES The manual scaling mode allows sections of the scan to
44. apter A Figure 4 on page 15 in cell compartment 2 em verification 7 Remove any cuvettes cells from the cell compartment and press Start verification 8 Insert the different filters in the given order one after the other Press Next after inserting a filter fog Saree eo fe ae stray Light Check insert filter 109 Advanced Operations Optic gt CSL za AS Yerification Stray Light Check KY450 3 PASS Nominal Value gt 2 8 Abs Photometrical Accuracy NG9 f1 PASS Nominal Value 1 493 Abs Actual Value 1 493 Abs 6 8 4 Output checks 6 8 5 Lamp history O wm Lamp History YISIBLE Hours 50 0 Reset YIS Cycles 62 NE 6 8 5 1 Factory service O wes Service Code 110 After the last measurement the results are displayed 9 Press the PC amp Printer icon to send the data to a USB memory stick PC or to a printer The files will be stored automatically as CSV file Comma Seperated Value The file name will be formatted as Verification csv If a printer is connected a test printing of the current screen will be printed The Lamp History menu provides the amount of time that the lamp has been on Hours After a lamp is replaced and the Lamp History is reset the display of the total operating time is reset to 0 1 Press Lamp History in the System Checks menu 2 Press Reset VIS and the Visible Lamp will be reset 3 Press OK to return to System Checks T
45. ata to a printer computer or USB memory stick Type A Integral On Off The integral gives the area and the derivative of the integral gives the original function 90 Advanced Operations Wavelength Scan WIS Options Cursor Mode CH Send Track Data EE Scale amp Units n Reference of A nm pas en eed RIL PABLO BF h Ret Instrument Options EL Setup Table 13 Wavelength scan setup options continued A A Scale In the automatic scaling mode the y axis is automatically adjusted so that the total scan is displayed The manual scaling mode allows sections of the scan to be displayed Units Choice of absorbance or transmittance Scale amp Units Call up saved measurement data wavelength scans or time courses see section 5 3 on Recall Data page 36 Instrument Setup Instrument Setup Basic data of the instrument see section 5 2 on page 25 y auri meth Onan me A E An o Wavelength EEE Setting wavelength 400 em 1 Press the key in the Options menu to select the wavelength range and the wavelength step Step 2 Press the upper left key to open the numeric keypad and select the minimum wavelength Press OK to confirm inm 2 nm C 5nm 3 Press the upper right key to open the numeric keypad and Cancel OK select the maximum wavelength Press OK to confirm PM Note Do not select the same wavelength for minimum and maximum 4 Highlight the required wavelength
46. ated or deactivated with the control boxes on the left of the Timer 3 Timer 4 display In the next column a selection can be made from a list of names that describe the corresponding work step In the third column the times for each active timer are entered 6 1 2 1 Measurement process The measurement process defines the handling and the measurements of the test e At which and how many wavelengths should measurements be completed e How many absorbance measurements must be completed e When should the zero measurement and the sample measurement be completed e Are waiting times necessary between measurements 68 Advanced Operations e Should individual program sequences be repeated The elements of a measuring Sequence such as zero and sample measurements and the timer s reaction times waiting times etc are individually defined 6 1 2 2 Enter a new element of a measuring sequence weg Measurement Process Users HEA Measurement Process Important Note Each component of the measurement process must be entered in the order in which it will be completed 1 Highlight the Measurement Process line in the data overview and press Edit 2 Press Edit again and then New Content and definition of the keys Z key Zeroing 1 Press the Z key to program a zero measurement Confirm with OK 2 Press New and then Zeroing and use the alphanumeric keypad to enter the wavelength at which the
47. cccncnnccnnncncocnnononos 83 6 4 1 Set up Single Wavelength mode n nnannnnnoannannnnnennonnsnrnnnrrnrorrsnrrnrrrrrrrrsrrsnrrrernrrorrsrrnnernernrrnernernrrerenneenen 83 6 4 2 Take single wavelength measurements single reading oocccocccccncccnccnnnnncncnononanonanonncnonononanonanonnnos 85 6 4 3 Take single wavelength measurements CONTINUOUS readings occcccccccnccocncccnncnnnonnnoncncnnncnncnnncnnnancnnos 85 6 5 Multi Wavelength mode measurements at more than one wavelength cccooccoonccccncocncconcnncnnncnnnnncnnnnnns 86 6 5 1 Set the reading mode at different wavelengths nannonnnnnnnnnnnnnnnnnnnnnnnnnnnnsrnnnrnnrnnrrnrrsnrnnrnnrnnrrsnrernrnrenne 86 6 5 2 Complete a measurement in the multi wavelength mode oocconcncccncoccncncnccnncncncnnconncnoncnonanonnnnonononanonnnos 89 6 6 Wavelength Scan mode recording of absorbance and transmittance SpectrUMS occcocccociccncocnconcccncnnnonnnonos 90 6 6 1 SeCUUD ING wavelength Scan dd na O 90 6 62 Wavelength Scan reading EE 93 6 6 2 1 Navigation of the wavelength scan graph or a wavelength scan analysis ooccoccccocncocncno 94 6 6 3 Work with reference scans EE 95 6 7 Time course Of absorbance transmittance ooccoocncccncconncocnnccnnnnnnnnnnnnnnnnnnnonnnnnnnnnnnnnnnnnnnnnnnnonnnnnnnnnnnnnnnnnnnaninaninns 97 6 7 1 Time course setup parameters aci Ee 97 6 1 2 TME COUrSE SCAM FEAGING EE 98 0 2 3 Analysis Orme Course datar ci
48. culating the concentrations Enter calibration formula C1 1 Highlight the Formula line in the data overview and press Edit 2 Highlight the line C1 Off and press Edit 3 Select C1 Off again and press Edit The display changes to C1 On 4 Highlight the next line C1 to define the formula and press Edit 71 Advanced Operations User testes ones Refer to Table 10 for detailed information on the Edit Edit Formula C 1 formula keys Note The evaluation formula is built up successively in the display in accordance with the input Select Abs Note The arrow key deletes the most recently entered element of the Conc Variables formula New F 2 Number x SE rre Abs Yariables Press the Abs Variables key to select from the displayed list the required element of the defined Abs Variables measuring sequence and therefore the corresponding measurement wavelength so that this can be taken into account in the formula e Cancel User paese MEA Wew Number Press New Number to enter a new factor or constant ca Cancel 72 Advanced Operations Table 10 Edit formula key descriptions continued Press x to enter a mathematical operation TT Select the operation and press OK to confirm Math operator The available choice of mathematical operations depends on the defined formula This means that functions such as In or log etc are only active if a term in pa
49. d Programs in the main menu Select the required program Abs Trans 2 Press Start 3 Analyze a sample without added standard solution in 22 SEP 2006 14 17 32 accordance with the instructions in the Procedures Manual F When the measurement is complete leave the sample cuvette cell in the cuvette sample cell holder Rom 4 CA send Reading Mode Data Single Dilution Standard Factor Off Additions Standard Chemical Form Adjust Off al Press Options gt More gt Standard Additions Reagent Blank Save as ES off User Program Instrument Return Setup An overview of the data of the standard addition procedure is displayed Optic P Standard Additions Standard Concentration 50 00 mg L AIS 5 Press OK to accept the standard values for standard concentration sample volume total and standard addition Sample Volume volume Press Edit to change any of these values 30 0 mL Spike Volumes mL 0 0 0 1 0 2 0 3 Cancel Edit OK 80 Standard Concentration 50 00 E GES Sample Volume Standard Additions mL moa L Standard io Recovery 6 7 Advanced Operations Press the key of the value to change it Use the alphanumeric keypad to change the value Press OK to confirm Press the keys to enter the standard addition volumes Use the alphanumeric keypad to enter the new data and press OK Description of the table of readings The first column shows the s
50. d in measurement mode In the results screen press Options gt More gt Instrument Setup If a Sample ID is already assigned select the Sample ID symbol in the results screen 1 Press Date amp Time in the Instrument Setup 2 The date and time are subdivided over a number of fields Press the appropriate field and use the arrow keys to change the value 3 Press OK to confirm The instrument will return to Instrument Setup 27 Standard Operations 5 2 4 Display and sound preferences Instr SR n pak geg DH Display amp Sound Display Brightness UA iy coe o Sound FT Screen touch W Reading done Timer Short Long 6 Instr SR n pak CP 28 Number of beeps 4 25 1 Press Display amp Sound in the Instrument Setup The following options will be displayed Display Brightness Adjusts the display brightness to suit lighting conditions Screen touch Activates Deactivates a short beep each time the screen is pressed Default off Reading done Activates Deactivates a sound when a reading is complete Default short beep every time a reading is complete Timer Adjusts the length of the timer sound Select Short or Long Long beeps are recommended for noisy environments Select Long to change the number of audio signals Use the alphanumeric keypad to enter specify the number of audio signals 4 25 Note A high number of audio signals increases the duration o
51. d the associated wavelength are highlighted in black e In addition the display shows the difference between the wavelength scan curve and the reference curve against the wavelength Press the black or orange small box in the left upper corner on the screen to switch between the actual wavelength scan and reference scan 95 Advanced Operations Reference Scan WIS Zt 400 nm 400 A 500 nm Difference 0 447 Main Menu Options 96 Second option Insert the blank cuvette cell into the cuvette sample cell holder Press Zero Insert the sample cuvette cell into the cuvette sample cell holder Press Read e The newly plotted wavelength scan curve is shown in black e The absorbance or transmittance value and the associated wavelength are highlighted in black Press Options and then Reference Off in the Options menu to select another scan to display on the same screen with the current scan Highlight the required scan number and press Highlight Reference Note After selecting a reference scan the Reference Off key in the Options menu turns into Reference On Note Only scans that have the same wavelength range and step can be displayed using the overlay option The reference curve is shown in orange The absorbance or transmittance value and the associated wavelength are highlighted in orange e Additionally the difference of the absorbance and or transmittance value between the two scans measured scan
52. ding absorbance values in the displayed table press the symbol Use the alphanumeric keypad to enter the value Press OK and enter the corresponding absorbance value Press OK The entered data are displayed in the table Repeat the sequence for each data point to enter it 3 To change a value in the table highlight the appropriate line press the unit key e g mg L or Abs and enter the changed value via the alphanumeric keypad Calibration Values 4 When the data have all been entered press Graph a Onn Note The correlation coefficient r2 is shown on the left below the a i axes 4 00 mg L Curve Fit r 1 0000 Calibration Yalues 5 The linear equation corresponds to the standard setting Press C a b c Next Curve to display the polynomial 2nd order curve Press ann Next Curve again to display the polynomial 3rd order curve Abs 6 Press Force 0 to change the setting from Off to On The curve now passes through the origin of the coordinate system Note This may have an adverse effect on the correlation coefficient r 4 00 mg L Curve Fit r 1 0000 7 Press Table to display the table again 8 When the table has been completed and the curve type has been chosen press Done when the graph is displayed or Exit when the table is displayed Go to section 6 1 1 4 on page 66 63 Advanced Operations weg Calibration C Enter Yalues CG Read Standards Enter Formula P
53. e If the stored program is deleted in User Programs it will also be deleted in Favorites Programs 75 Advanced Operations 6 1 4 1 Add to Favorites A giereg Add to Favorites Program 950 User Program Add to Favorites ay D OK A 6 1 4 2 Edit User Program 950 Name User Program Cancel 6 1 4 3 Delete wg Delete Program Program 950 User Program Delete Program dy ores OK 76 Press Add to Favorites and press OK to confirm The program is added to the Favorites Press Edit and press OK to confirm An overview of the specifications of the programmed test is displayed More information about the input options is provided in the section 6 1 2 on page 68 Press Delete and press OK to confirm The program is deleted from the list of User Programs Note If the stored program is deleted in User Programs it will also be deleted in Favorites Programs 6 2 Favorite Programs 6 2 1 Recall a favorite program Favorite Programs 351 WN Nitrate LR 0 50 mod 950 User Program Main Select by Remove Menu Number Program 6 2 2 Delete a favorite program O wg Remove Program 951 950 Program 950 User Program Remove from Favorites ay D OK A Advanced Operations The most frequently used tests methods in the Stored Programs menu and the User Programs menu can also be added to the list of favorites to simplify their selection To add Stored Programs and or Use
54. e section 6 1 2 on page 68 Table 7 Program descriptions C Free Programming Program Type Description Single Wavelength Measurements at a defined wavelength f In the Multi Wavelength mode absorbance values can be measured at up to four wavelengths Multi Wavelength i l and the results can be mathematically processed to obtain sums differences and relationships This is an advanced form of programming for original user developed methods In Free Free Programming Programming the user defines the measurement process variables and calculations involved in obtaining a reading 58 6 1 1 1 Single wavelength settings wm Linits ce Cancel OO wemmer Wavelength A nm 7 User wm Concentration Resolution C 0 001 ca Cancel Back Next L Fee Advanced Operations If the Single Wavelength mode is selected the following parameters can be defined Units Select the required units from the list and press Next Note Units of measure not included in this list can be added in the edit program under Program Options Edit Select Units Edit and then New Wavelength single wavelength program type Use the alphanumeric keypad to enter the measurement wavelength The entered wavelength must be in the range from 320 1100 nm Press Next to proceed Concentration resolution number of decimal places Select the required number of decimal places from the displayed list and press Next 59 Advanced
55. e and multi wavelength mode A method is calibrated by determining the absorbance values of several standard solutions of known concentration There are three ways to create and store a calibration curve Instructions for each method follows Table 8 Table 8 Calibration settings ee A calibration table is created by entering the concentration values and the absorbance values of Enter values the analyte solutions The absorbance values are plotted versus standard concentrations and the calibration curve is displayed as a graph page 62 A calibration table is created by entering the concentration values of the standard solutions and Read Standards then measuring the absorbance of the analyte solutions The absorbance values are plotted versus Standard concentrations and the calibration curve is displayed as a graph page 64 If the calibration curve can be determined from the mathematical relationship between concentration and absorbance by linear regression etc the corresponding formula can be selected linear 2nd or 3rd order polynomial from a list and the appropriate factors can be entered page 65 Calibration by entering calibration values Entering concentration absorbance LI Fi ur ra aa m ann Cri aiid Calibration 1 Select the Enter Values and press Next CG Enter Yalues Read Standards C Enter Formula 62 Advanced Operations Calibration Yalues 2 To enter the standard concentrations and correspon
56. e as ES off User Program lA SER 2006 14 49 13 Instrument Setup Table 4 Stored programs options Description For further Options With the Store On setting all measurement data are stored automatically With the Store Off Store Off On setting no measurement data are stored Trans Conc Abs To switch to transmittance concentration or absorbance readings Send Data icon Send Data To send data to a printer computer or USB memory stick Type A The Help Guide is an accessory available on a USB memory stick see section 5 2 6 4 on Information icon page 33 Timer icon This functions as a stopwatch It helps to ensure that the steps of an analysis are correctly timed e g reaction times wait times etc can be exactly specified When the specified time has elapsed an acoustic signal is emitted The use of the timer has no influence on the measurement program Single Reading Mode A reading is only displayed after a measurement has been carried out press Read standard setting see section 5 4 4 1 on page 45 Continuous Reading Mode After the zero measurement all readings are displayed automatically and continuously see section 5 4 4 2 on page 45 Reading Mode A corrective dilution factor can be entered in order to take account of certain properties The number entered at the dilution factor prompt will be multiplied by the result to compensate for the adjustment For example if the sample has be
57. e data are available for formatting changes These include automatic scaling and zoom functions Maximum and minimum values are determined and shown as a table The cursor can be moved to any point on the curve for the purpose of reading off the absorbance or transmittance value and the wavelength The data associated with each data point can also be shown as a table 6 6 1 Set up the wavelength scan Press Wavelength Scan in the Main Menu Press Options for Parameter Setup Wavelength Scan OTS Options Cursor Mode CH Send Track Data E Scale amp Units n Reference ing 25 SEP 2006 11 10 07 Table y Ret Instrument Options sd Setup Table 13 Wavelength scan setup options CI om o O From the displayed list of stored scans a record is selected for use as a reference scan superimposed scan This can be highlighted or shown in the background in comparison Reference Off On with the actual measured scan Note This option is only available when there are stored scans with the same wavelength range and step To enter the wavelength spectrum and the scan interval Enables the user to switch the display back and forth between the scan data tables Select View wavelength absorbance and the graph of the curve Note Select View will be activated after the first reading To select Track or Peak Valley The selection for this menu item determines to which points on Cursor Mode the graph the cursor moves Send Data To send D
58. ears in order to configure the functions De mar oi the instrument O Lamp CH PC amp Me Control Printer 1 Select Instrument Setup in the Main Menu Password EH Select Color de Main Menu 5 2 1 Operator ID Use this option to enter up to 30 sets of operator initials up to five characters each into the instrument This feature helps record which operator measured each sample weg Operator ID 1 Press Operator ID in the Instrument Setup 2 Press New to enter a new Operator ID 3 Use the alphanumeric keypad to enter a new Operator ID 4 Press OK to confirm Instr st ete Select one of the four preset color palettes in the Select Color menu Select Color to assign to the Operator ID 5 Select a color category to highlight the color for the display background Note Press Cancel to select the default setting 6 Press OK to confirm 25 Standard Operations Inst pet Ct te Operator ID Neu Delete OK 5 2 2 Sample ID Instr eeu ent Cte a Sample ID Instr een ent Ce a Sample ID Cancel 26 7 The display shows the chosen Operator ID 8 Press OK The instrument will return to the Instrument Setup screen and show the selected operator identifier 9 The chosen Operator ID is activated Note Press Delete to remove an Operator ID from the list Note Alternatively enter or change an Operator ID in measurement mode In the results screen press Options gt More gt
59. elength Scan 24 38 39 40 41 90 91 94 Wavelength Setting coococcocccoccccccnccnccncnononanos 97 Wavelength Spectrum 90 130
60. en diluted by a factor of 2 enter 2 The default setting of the dilution factor is turned off Note When a dilution is in effect the dilution icon will appear on the display Dilution Factor Off On This enables the accuracy of the measurements to be checked The working procedure for a test parameter contains a detailed explanation of how to use this function Standard Addition The working procedure for a test parameter indicates whether a standard adjustment is Standard Adjust necessary and if so how to proceed Chemical Form Some of the stored tests methods allow to select the chemical form and the measuring range Some of the stored tests methods include the Reagent Blank function This enables the reagent blank value to be added to or subtracted from the subsequent readings The reagent blank value Reagent Blank shifts the calibration curve along the y axis without changing the shape or gradient of the curve The effect corresponds to a y axis intercept of the calibration straight line This is made clear by the following equation Concentration Conc factor Abs reagent blank value To store the selected parameters as a User Program Save as User Program Recall Data Call up saved measurement data wavelength scans or time courses see section 5 3 on page 36 Instrument Setup Basic data of the instrument see section 5 2 on page 25 Standard Operations 5 4 3 Use of program timers D I D
61. enance 7 1 Cleaning requirements 7 1 1 Spectrophotometer 7 1 2 Display 7 1 3 Cuvettes sample cells CAUTION Potential Chemical Biological Eye and Skin Hazards Only qualified personnel should conduct the tasks described in this section of the manual Important Note Remove any cuvettes cells that are still in the instrument and dispose of them or their contents using an approved disposal method CAUTION Potential Pinch Eye Burn and Chemical Hazards Always disconnect power from the instrument before attempting any cleaning operations Important Note Under no circumstances should the instrument display or the accessories be cleaned with solvents such as white spirit acetone etc e Clean the enclosure cuvette sample cell compartments and all accessories with a soft damp cloth A mild soap solution can also be used Do not get excess water in the cuvette sample cell compartments Do not insert a brush or sharp object into Cell Compartment 1 to avoid damaging the mechanical components e Dry the cleaned parts carefully with a soft cotton cloth e Take care not to scratch the display Do not touch the screen with ball pens pencils or similar pointed objects e Clean the display with a soft lint free and oil free cotton cloth Diluted window cleaner liquid can also be used CAUTION Potential Chemical Biological Exposure Hazards Use proper laboratory practices whenever there is a risk of chemical exposure
62. f the tones and a small number of audio signals reduces the duration of the tones Press OK to confirm The selected number of the audio signals sounds as a corresponding acoustic signal Press OK to confirm The instrument will return to Instrument Setup 5 2 5 Lamp control sai me mban Lamp Control YIs Lamp GG On C Off W Save 2 Hours sai me DA Yisible Lamp Save 1 Hour 2 Hours 4 Hours 5 Hours 12 Hours gm o Standard Operations The tungsten lamp produces light in the wavelength spectrum 320 to 1100 nm The life span of the halogen lamp depends on the burning duration In order to extend the life span of the lamp switch on the Lamp control e Ifthe instrument is not used during a longer period 1 12 hours e Ifthe instrument will never be switched off 1 Press Lamp Control in the Instrument Setup 2 Select On to switch on the Lamp 3 Select Save in order to define a time interval for the burning time of the lamp 4 Press the field below Save to select the lamp burning time 5 Select the length of time the lamp will be switched on Note After this period of time the lamp will automatically turn off after no measurement has been made Note The lamp will be restarted automatically for measurements 6 Press OK to confirm 29 Standard Operations 5 2 6 PC and printer The instrument is provided with 2 USB interfaces which are located on the back of the instr
63. gh the sample and reaches the detector is measured Concentration measurements A concentration factor can be entered to enable the measured absorbance values to be converted into concentration values In the multi wavelength mode absorbance Abs or percent transmittance T is measured at Multi Wavelength up to four wavelengths and absorbance differences and absorbance relationships are calculated Simple conversions into concentrations can also be carried out A wavelength scan shows how the light from a sample is absorbed over a defined wavelength spectrum This function can be used to determine the wavelength at which the maximum absorbance value can be measured The absorbance behavior is displayed graphically during the scan Wavelength Scan Time Course The time scan records the absorbance or transmittance at a wavelength over a defined time The system checks menu offers a number of options including optical checks output checks lamp history instrument update service time and instrument backup Recall Data Stored data can be recalled filtered sent and deleted In this mode user specific or method specific settings can be entered Operator ID Sample ID Date amp Time Display amp Sound Lamp Control PC amp Printer Password and Select Color System Checks Instrument Setup 24 Standard Operations 5 2 Instrument Setup mode Instrument Setup a mp lt Off gt lt Off gt A selection of functions app
64. grams The instrument contains more than 200 programmed procedures Most can be accessed through the Stored Programs menu 5 4 1 Select a saved test method entering user specific basic data Stored Programs 1 Press Stored Programs in the Main Menu to view an alphabetical list of stored programs with program numbers Aluminum Alumin Alurmmum ECR The Stored Programs list will appear 2 Highlight the required test Note Select the program number by name or use the arrow keys to scroll through the list quickly and highlight the program or press Select by Number to search for a specific program number Use the Main Select by Program alohanumeric keypad to enter the test number and press OK Menu Number Options 3 Press Start to run the program After a program is selected the screen for that parameter will appear Note All corresponding data wavelength factors and constants are already preset 4 Follow the chemical procedures described in the corresponding Procedures Manual 5 4 2 Stored program options 1 From the Main Menu select Stored Programs Select the necessary method and press Start 2 Press Options for Parameter Setup Refer to Table 4 on page 43 for stored program descriptions 42 Standard Operations 10 Aluminum Alumin WIS Lt Options Send Reading Mode al Data Single le Dilution Standard Factor Off Additions A Standard Chemical Form LA Adjust Off al Reagent Blank Sav
65. gth Reproducibility lt 0 1 nm Wavelength Resolution 1 nm Wavelength Calibration Automatic Wavelength Selection Automatic based on method selection Scanning Speed gt 12 nm s in steps of 1nm Spectral Bandwidth 5 nm Photometric measuring range 3 0 Abs wavelength range 340 900 nm 5 m Abs at 0 0 to 0 5 Abs 1 at 0 50 to 2 0 Abs lt 0 5 to 2 Abs lt 1 at gt 2 Abs with neutral glass at 546 nm Stray Light lt 0 1 T at 340 nm with NaNO Photometric Accuracy Photometric Linearity Data strorage 1000 measured values Result Date Time Sample ID User ID User programs Physical and environmental specifications 368 mm 14 5 in 144 mm 5 7 in wee eneen 10 40 C 50 104 F max 80 relative humidity non condensing 40 60 C 40 140 F max 80 relative humidity non condensing Additional technical data Mains connection DR 3800 connected with External power supply 100 240V 50 60Hz input 15V 30VA output Use only shielded cable with max length of 3 m Interfaces 1 x USB type A 1 x USB type B Enclosure rating IP3X Instrument Class III Safety class System Class II Specifications Section 2 General Information 2 1 Safety information 2 1 1 Use of hazard information 2 1 2 Precautionary labels Please read this entire manual before unpacking setting up or operating this equipment Pay attention to all danger warning and caution statements Fai
66. he Factory Service menu is password protected This menu is not intended for customer use Advanced Operations 6 8 6 Service time In order to ensure a regular inspection an automatic memory reference for the service times can be entered After switching the instrument on this memory reference will be activated and indicated at the appropriate time Service Time 1 Press Service Time in the System Checks menu 2 Select On and then Last Service to enter the date of the last Last Service inspection 6 JUN 2006 3 Press OK to confirm Next Service 26 JUN 2007 12 Month ho ea Next Service 4 Select Next Service to determine a specific period of time up to the next inspection C 3 Month 5 Press OK to confirm C 6 Month 12 Month If the next service is due the message Next service is due is Main SITAS displayed after switching on the instrument 6 Press OK to return to the Main Menu Next Service Contact the manufacturer or distributor to arrange an appointment is due for the next service 111 Advanced Operations 6 8 7 Instrument backup Before the next service date the Instrument Backup menu offers the possibility to store all programs measuring data Operator ID Sample ID passwords and all adjustable data on a USB stick AS mh Ale H Instrument Backup 1 Press Instrument Backup in the System Checks menu Restore 2 Connect the USB memory stick section 3 4 o
67. ight bracket marks the end Note The key showing the right bracket remains inactive until a left bracket is entered 1 Press 2 Press the key that defines the sequence that is to be repeated Z or R Confirm with OK 3 Press New and then press Zeroing or Reading and use the alphanumeric keypad to enter the wavelength at which the measurement should be completed Press OK and confirm the input by pressing OK again 4 Press to end the sequence Note If an action such as a zero measurement recurs at different stages of a measurement sequence the series of actions is numbered sequentially e g Z1 Z2 etc A Measurement Process es Neu Delete OK DSS Advanced Operations Deleting an element of a measuring sequence Select the appropriate line and press Delete The element is deleted Inserting an element of a measuring sequence Select the line in the measuring sequence where the insertion is be made and press New A new element can be entered at the selected position When the input is complete press OK in the Measurement Process display The data overview is then displayed 6 1 2 3 Enter the calibration formula evaluation formula User Program 951 Cancel User Sects DEZ Formula The calibration formula evaluation formula defines the calculation and display of intermediate and final results The previously defined elements of the measuring sequence are the basis for cal
68. ing program type Free Programming is an advanced option for entering original user developed methods When the Free Programming option is selected an overview of the specifications of the programmed test is displayed Each input option can be modified to develop the user method Refer to Table 9 for more information To modify an input option select the appropriate line and press Edit Important Note Perform steps 1 6 of Programming a New User Method section 6 1 1 on page 57 before proceeding with Free Programming User Program 951 Highlight the line containing the program point that is to be edited or Mame FP defined and press Edit Cancel Table 9 Definitions of the program points Name Name of the analysis parameter An abbreviation or version number assigned by the user is entered here Exact definition of the test the number of wavelengths at which measurements are made the Measurement Process number of absorbance measurements needed the keys to be used any waiting periods between measurements etc Definition of the formulas with which the test result is calculated The number of variables shown in the display depends on the definition of the measurement Variables process and the formulas Input of the numerical values of the wavelengths factors constants etc Used to enter abbreviations and defined times for up to four timers Highlight the appropriate line Timer 1 Timer 2 and press Edit The timers are activ
69. ing range limit Confirm the entry by pressing OK 66 ice User men Timers men Chemical Forms Advanced Operations Timer 1 Timer 2 Timer 3 Timer 4 This function can define time intervals for up to four timers Timer designations such as Shake Wait and Swirl can be assigned 8 Highlight the appropriate line in the overview of the program data and press Edit The timers are activated or deactivated with the check boxes in the left part of the display In the next column a selection can be made from a list of names that designate the corresponding work step In the third column the times for each timer are entered in mm ss Chemical form 2 chemical form 3 chemical form 4 If a Chemical Form 1 has been defined up to three additional alternative forms can be entered here 10 11 12 13 Highlight the appropriate line in the overview of the program data and press Edit The chemical forms are activated or deactivated with the check boxes in the left part of the display Press the left key to enter another chemical form with the alphanumeric keypad and press OK to confirm Press the right key to enter the conversion factor to calculate the concentration of the additional chemical form from the concentration of Chemical Form 1 and press OK to confirm Press Store to save the program data Press Cancel to return to the Main Menu 67 Advanced Operations 6 1 2 Free programm
70. is folder 4 Use the USB memory stick to update the instrument software 6 1 3 Select a user program User Programs 950 User Program Select by Program Number Options 3 Advanced Operations Press User Programs in the Main Menu to view an alphabetical list of user programs with program numbers The User Programs list will appear Highlight the selection by pressing it or press Select by Number to search for the program by number Note Use the scroll bar to scroll through the list quickly Use the alphanumeric keypad to enter the test number program number and press OK to confirm Press Start to run the program 6 1 4 Add edit and delete user programs from the favorites list User Programs 950 User Program Select by Program Number Options es s Program Options 950 All 350 en Add to Favorites Edit Delete Cancel The most frequently used tests methods in the User Program menu can also be added to the list of favorites to simplify their selection Press User Programs in the Main Menu The User Programs list will appear Highlight the selection by pressing it or press Select by Number to search for the program by number Note Use the scroll bar to scroll through the list quickly Use the alphanumeric keypad to enter the test number program number and press OK to confirm Press Program Options Press Add to Favorites Edit or Delete and press OK to confirm Not
71. l power supply into an electrical outlet 2 Turn the instrument on by pressing the power switch on the back Note Do not turn the instrument off and on in rapid succession Always walt about 20 seconds before turning the instrument on again otherwise the electronic and mechanical systems will be damaged The spectrophotometer software includes several language options The first time the instrument is turned on the language selection screen will appear 1 Select the desired language 2 Press OK to confirm the language selection The self check will start automatically Changing the language setting The instrument functions in the selected language until the option is changed 1 While turning the instrument on touch the screen at any point until the list for selecting a language appears about 30 seconds 2 Select the required language 3 Press OK to confirm The test program subsequently starts automatically Each time the instrument is powered up a series of diagnostic tests is performed automatically to ensure operation of major system components This procedure which takes approximately two minutes checks the system lamp filter adjustment wavelength calibration and voltage Each test that functions correctly is confirmed with a check mark The Main Menu is displayed when power up diagnostics are completed Note Further error messages during self check see Section 8 on page 121 21 Start Up
72. la ii 23 35 115 Display ANd Sound ccceccseeecseeceeeceeseeeeeeesenesens 28 BIC DOS AM illes 10 PPM Ne Che util eo 107 E EANG TOSS tds 50 F Facto SEVICE aa n Ar A 110 FAV ONICS eebe e 24 50 76 77 A ace 76 77 GOING EE 76 recalling ainda 17 Fiter Settings EE 37 Free programming ococccccccccnccccnnnnno 68 69 71 74 76 H HAGA Data Man usina 37 Hazard Information 9 Instrument Backup 112 113 Instrument Information 101 Instrument Setup 24 25 43 54 84 87 91 97 Medrano a ias 90 92 NWOT le 30 MEET asadas 56 102 L Lamp see VIS Lamp EAU NG ACS sans sr ns cata ld 21 M Man MENU ir ee 24 Maintenance viii 115 Mai data 99 Measurement Process c ooccocccocccoccnocconccononononanennonos 68 Measuring Sequence aessssissrrrrcrerrrrrrrrrrrerrerrrrrrrrrree 69 Multi Wavelength 24 60 62 86 87 88 89 N Klein 103 O Operator Benne ene ete ee ee deen 25 lt WEE 25 SE ln Le ME 26 Optical Checks 102 103 105 106 107 108 OUIDUL CHECKS deed 110 Overview of Product FUNCTION ooccocccocccconcccncononoso 10 P PASS WOl GO ita lio 33 A dee E 33 dSaCcU te Le EE 35 FO and MINE alii 30 32 PAmer Setup ee Ee 30 FIRING data an 32 Programm OVOIVIOW 24 Beieleit ul Le EE 57 Reading Mode 43 45 84 85 Reagent Blank 43 48 Recall Data 24 39 43 54 84 87 91 97 REfErENCE SCAN arie a e 90 95 S Safety Information
73. lure to do so could result in serious injury to the operator or damage to the equipment To ensure that the protection provided by this equipment is not impaired do not use or install this equipment in any manner other than that specified in this manual DANGER Indicates a potentially or imminently hazardous situation which if not avoided will result in death or serious injury WARNING Indicates a potentially or imminently hazardous situation which if not avoided could result in death or serious injury CAUTION Indicates a potentially hazardous situation that may result in minor or moderate injury Important Note Indicates a situation which if not avoided may cause damage to the instrument Information that requires special emphasis Note Information that supplements points in the main text Read all labels and tags attached to the instrument Personal injury or damage to the instrument could occur if not observed A symbol if noted on the instrument will be included with a danger or caution statement in the manual This symbol if noted on the instrument references the instruction manual for operation and or safety information Electrical equipment marked with this symbol may not be disposed of in European public disposal systems after 12 August of 2005 In conformity with European local and national regulations EU Directive 2002 96 EC European electrical equipment users must now return old or end of life equipment to
74. n Factor Off Resolution 0 01 Absorbance Save as Formula User Program a BA h Instrument Options Return Setup Table 12 Multi wavelength setup options COC Store Off On With the Store On setting all measurement data are stored automatically With the Store Off setting no measurement data are stored Trans Abs To switch to transmittance concentration or absorbance readings 2 Wavelenath To enter the measurement wavelength Use the alphanumeric keypad to enter the measurement 9 wavelength The entered wavelength must be in the range from 320 1100 nm This functions as a stopwatch It helps to ensure that the steps of an analysis are correctly timed e g reaction times wait times etc can be exactly specified When the specified time has elapsed an acoustic signal is emitted The use of the timer has no influence on the measurement Timer icon program Concentration Factor Multiplication factor for converting absorbance values into concentration values Concentration To select the position of the decimal point in the calculated concentration readings Resolution Absorbance Formula Calculation basis for evaluating samples Save as User Program To store the selected parameters as a User Program 86 Advanced Operations Multi Wavelength VIS Options Concentration Concentration Factor Off Resolution 0 01 Absorbance Save as Formula User Program Trans 2 e 11 03 12 Instrument Return Se
75. n Print quality e Paper Paper size Note If an optional Thermal Printer is connected the function Auto Send on off is available 4 Select Auto Send On to send all measured data automatically to the Thermal printer Note The option Auto Send is not available for any other printer e g ink jet printer 5 Press Resolution to select the print quality Select between e 100 dpi e 150 dpi and e 300 dpi 6 Press OK to confirm Note Press OK again to return to the PC Printer menu 31 Standard Operations Data Log 37 11 S5EP 06 14 16 00 1004 o mal DCO 15 SEP 06 09 15 04 Ww 05 02 3 93 mg l P 4 P CH Sending Data 15 SEP 06 10 08 00 Wiw 05 05 0 000 mg L Al Main Filter Viea h Menu Off Details Options 5 2 6 3 HACH Data Trans Inst poet SA A PG amp Printer y Printer Deskjet 6500 A PC Connected 5 Cancel Setup OK 32 7 Press Paper to select the paper size Select between e Letter e Legal e Executive e A4 8 Press OK to confirm Note Press OK again to return to the PC Printer menu 1 Press Recall Data in the Main Menu 2 Select the data source where the data to be printed are stored 3 A list is displayed Data can be filtered For more information see section 5 3 1 2 on page 36 4 Press the Printer icon to send the data table curve immediately to the printer 5 Highlight Single point or Filtered data or All data and press OK
76. n factor prompt will be multiplied by the result to compensate for the adjustment For example if the sample has been diluted by a factor of 2 enter 2 The default setting of the dilution factor is turned off 2 Press OK to confirm Press again OK 3 Press Return to return to the result display 4 Confirm Store present reading again to save the current measured value if necessary Note When a dilution is in effect the dilution icon will appear on the display Note If using undiluted samples set the dilution factor off again 5 4 6 Run a standard adjust Optic Standard Adjust Current Reading 0 110 Adjust to 0 127 mg L Al 10 Aluminum Alumin 0 127 3 EE EE 5 4 7 Set the chemical form S22 nm Standard Operations The Standard Adjust function allows the calibration curve for a stored program to be adjusted based on analysis of a known standard solution The Accuracy Check section of written procedures often suggests a standard solution concentration for this purpose Read a standard before setting Standard Adjust to On 1 Follow the entire procedure using a known standard for the sample 2 After reading the concentration press Options gt More gt Standard Adjust 3 If Standard Adjust is set to Off turn it On The Current Reading will show the concentration The box on the right will show the default standard value for the test as mentioned in the procedure 4 If
77. n page 13 3 Press Store to start a Backup Note If the USB stick is not connected the message Please insert USB Memory is displayed Connect a USB stick in order to store the data Press OK to confirm and press Store again Please insert USB Memory Note Ifthe Backup was already stored before the message Data already exists Overwrite is displayed Press OK to overwrite the data already exist Overwrite by cre oe 112 Advanced Operations If the file was stored the message Instrument Backup is stored to USB memory will be displayed 4 Press OK to return to the System Checks menu str mL al Instrument Backup Restore backup data Important Note All current data will be overwritten when restoring the Backup file 1 Press Instrument Backup in the System Checks menu 2 Connect the USB memory stick containing the Backup section 3 4 on page 13 3 Press Restore to pass back the data Note If the USB stick is not connected the message Please insert USB Memory is displayed Connect a USB stick in order to store the data Press OK to confirm and press Restore again Restore Please insert USB Memory 113 Advanced Operations GU idee EK A zs Restore strument restored 114 4 Press OK to confirm after the message Instrument Backup from S N XXXXXXX Restore is displayed 5 After the backup start the instrument again Section 7 Maint
78. ng C Continuous Readings PUES A ee SES 1 Insert the blank cuvette sample cell into the cuvette sample cell holder Press Zero mg l 0 R 1 0 8 Al Note The Read key is only active after the zero measurement has been carried out 2 Insert the sample cuvette cell into the cuvette sample cell ann holder Press Read 13 OCT 2006 11 45 55 Note For data storage see section 5 3 1 on page 36 EL Ze 1 Insert the blank cuvette cell into the cuvette sample cell holder Press Zero 0 057 27 E Al Note In the reading mode Continuous only the Zero key is shown to start the reading The reading sequence is started automatically 2 Insert the sample cuvette cell into the cuvette sample cell f iii holder ds T 006 11 46 13 3 Press Options and then the Store icon to store the displayed data in the Data Log Note For data storage see section 5 3 1 on page 36 45 Standard Operations 5 4 5 Set the dilution factor 10 Al oras dono oca CN lo geess le y Store Data store present reading again Cancel OK A el 10 Aluminum Alumin NES 522 nm 0 111 7 Che K 15 SEP 006 14 50 01 46 The Dilution Factor function is used to calculate the original concentration of a sample that has been diluted by a known ratio For example dilution factor can bring the analyzed concentration within the test range 1 Press Options gt More gt Dilution Factor The number entered at the dilutio
79. nk depending on the working procedure into the cell compartment In the result display press Options gt More gt Chemical Form A list of available evaluation forms appears Select the new default setting Press Save as Default The current result and all further measurements will be displayed in the new chemical form Some of the stored tests methods include the Reagent Blank function This enables the reagent blank value to be measured and then taken into account in calculating the measurement result Measurement analysis of a reagent blank 1 Prepare the test method in accordance with the working procedure Instead of a sample deionized water is used to determine the reagent blank value Select the test If required by the working procedure insert the cell with deionized water into the cell compartment Press Zero Insert the prepared sample cuvette cell into the cell compartment Press Read The result is displayed 0 010 Adjust to 0 000 mg L Al oma o e 10 Aluminum Alumin REESEN 0 100 15 SEP 2006 15 23 54 5 4 9 Analysis of samples 10 Aluminum Alumin 0 000 m e 15 18 52 al Options 8 Standard Operations Press Options gt More gt Reagent Blank Press On to highlight the Reagent Blank function The concentration shown on the key is the measured value of the reagent blank To use this value for more analyses of this parameter press OK If the measureme
80. nt does not need to be saved press the key and use the alphanumeric keypad to enter a previously recorded reagent blank value Press OK Note The Reagent Blank function is deactivated when the measurement program is left To use the same blank value later for other tests using the same reagent lot enter the value per step 7 Note The results calculated using the reagent blank value must lie within the limits of the measuring range of the test method Note The reagent blank icon is shown in the result display see arrow when the function is active 1 2 3 Press Stored Programs and select a program Insert the blank cuvette into the cuvette sample cell holder Press Zero 49 Standard Operations 10 Aluminum Alumin 0 109 21 EE 15 19 01 Remove zero solution and insert sample cuvette cell into the cell compartment Press Read The result will be displayed For data storage see section 5 3 1 on page 36 5 4 10 Add stored programs to the favorite programs list Storp Pe2as gt nae Add to Favorites Program 510 Chlorine Ozone 4dd to Favorites fy ee Be 50 The Favorites menu simplifies test selection by creating a list of the most frequently used tests from the Stored Programs and User Programs Press Stored Programs in the Main Menu The Stored Programs list will appear Highlight the selection by pressing it or Select by Number to search for the program by number Press Add
81. nto the cuvette sample cell holder 93 Advanced Operations Wavelength Scan 3 Press Zero Zeroing appears below the graph as the baseline scan begins 4 Insert the prepared sample cuvette cell into the cuvette sample cell holder 5 Press Read Below the graph Reading appears and a graph of the absorbance or transmittance values at the scanned wavelengths is displayed continuously 400 Reading Main Menu Options Wavelength Scan WIS qi The Wavelength Scan is complete if e the graph is shown full size e the scaling of the x axis fits automatically e the Cursor functions in the vertical navigation bar are highlighted A 500 nm Integral 323 54 Options 6 6 2 1 Navigation of the wavelength scan graph or a wavelength scan analysis Table 14 Navigating the wavelength scan Cursor Function Deseriotio Zoom Function H Curve Icon Choice of Cursor Mode Peak Valley cursor moves between minimum maximum absorbance Choice of Cursor Mode values or Cursor Mode Tracking cursor moves over each data point of the scan The arrow keys are used right left to move the cursor depending on the selected mode to the next data point The data of the data point wavelength absorbance or transmittance value are highlighted on the x and y axes Note Press any point on the curve to display the associated data This function is used to magnify the section of the curve in the vicinity of the cursor The o
82. ogram MeS a il 44 94A Set mete adding Mode ti co 45 5 4 4 1 Take single wavelength measurements single reading 45 5 4 4 2 Take single wavelength measurements continuous readings occcocccocccoonccncnconoconnnonnnnnnnnnos 45 DAD SEEMNE CIUTION TACO ti a ida 46 AO Runa standard aos da nido 47 DA Sethe chemical TOn DER 47 5 4 7 1 Change of the default setting of the chemical form 48 540 RUM a TECATE AN e o e a 48 EE EIERE deed geed Eege 49 5 4 10 Add stored programs to the favorite programs list oooccoccccocccccnoccnnnonnnnononononnconncnnnnnonaronanonnnnnnnos 50 90 Barcode E ee EE 51 9 9 1 Complete a Darcode 13 mm BE RE 52 9 9 2 SEICCU the MEAS UNINC Tandil 53 5 5 3 Select the chemical evaluation form n nnannnnnannnanannnnnnnnnrnonrnnrnrnrrrrnrrnnrnnrrrnrnrernronrrnrrnrrnrronrrnnrnrrrnrnnenne 53 5 5 3 1 Change of the default setting of the chemical form 53 5 0 4 Basic test specific and sample specific data settings ooocccocccocncocnoconnconnnnnnonnnonnnonnnnonncnnnconaninnncnnnos 54 EE A lt P Ee 55 39 6 Update ota Darcode OSU isto ini ge Bedeelegte 55 5 0 7 Upgrade of ine Instrument Sotiware iii a 56 Section 6 Advanced Operations oocoocccoccocncccnconcconcocncncncnnnnnnnnnnnnnnnnnnnrnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnrnnnnnnnnennnnancnaninns 57 AI all EE 57 O Kall Programi REEL Lee EE 57 6 1 1 1 Single wavelength settings ia a lleida 59 6211 2 MUN Wavelength Seting EE
83. on Curve Icon Choice of Cursor Mode Arrow keys 100 a time scan or a time scan analysis After a time scan has been completed the time and the absorbance transmittance data are displayed as a curve Where the cursor is positioned on the curve the elapsed time up to this point and the corresponding absorbance are highlighted Table 16 Navigating the time scan Delta mode A second cursor is highlighted The position of the fixed cursor was previously defined in Cursor Mode Single Use the active cursor to select any point on the measurement curve The difference to the fixed cursor is shown on the curve The delta values are correspondingly highlighted and displayed on the x and y axes The gradient of the curve and the correlation coefficient r2 between the cursor points in the Delta mode are shown under the curve Cursor Mode Single The cursor moves to each selected measurement point of the scan The arrow keys right left are used to move the cursor depending on the selected mode to the next data point The data of the data point wavelength absorbance or transmittance value are highlighted on the x and y axes Note Press any point on the curve to display the associated data This function is used to magnify the section of the curve in the vicinity of the cursor The original curve size can be restored by pressing the zoom icon again Advanced Operations 6 8 System checks Main Menu 1 Press System Check
84. on Chemical SC Abs Factor Off PO Trans Save as User Program Aaa 14 09 05 h Instrument Options Return Setup Table 5 Barcode program options ian More For For further Options For further Options DO E the Store On setting all measurement data are stored automatically With the Store Off Store Off On setting no measurement data are stored Abs Abs Trans Trans To switch to To switch to transmittance or absorbance readings transmittance or absorbance To switch to transmittance or absorbance readings er Data icon Send Data To send data to a printer computer or USB memory stick Type A This functions as a stopwatch It helps to ensure that the steps of an analysis are correctly timed e g reaction times wait times etc can be exactly specified When the specified time has elapsed an acoustic signal is emitted The use of the timer has no influence on the measurement program Timer icon Information icon The Help Guide is an accessory available on a USB memory stick see 5 2 6 4 on page 33 A corrective dilution factor can be entered in order to take account of certain properties The number entered at the dilution factor prompt will be multiplied by the result to compensate for the adjustment For example if the sample has been diluted by a factor of 2 enter 2 The default setting of the dilution factor is turned off Note When a dilution is in effect the dilution icon will appear on the
85. or improved using the standard addition method This method also referred to as spiking serves to identify sample specific interference factors e g substances in the sample that falsify the analysis sample matrix effect a defective measuring instrument or contaminated reagents Method A defined amount concentration of a standard solution of the test substance is added to the sample The detection rate should be close to 100 Measured value after a standard addition PRE ONaO Expected value after a standard addition Detection rate Conclusion 6 Probability that the measurement results are correct EE is high Assumption The analysis was falsified by substances in the sample sample matrix effect Test to determine whether a sample matrix effect is present Use distilled water instead of the sample Add standard solution as described in the procedure Detection rate Conclusion lons in the sample are interfering with the analysis causing false results to be obtained No interfering ions consider other interference factors Measures to identify other interference factors Checklist 1 Check if the procedure is completed correctly a Are the reagents added in the correct order b Is enough time allowed for color development c ls the correct glassware in use d Is the glassware clean Advanced Operations e Does the test require the sample to be at a certain temperature f Was the p
86. parated Value files through a USB memory stick to a file named DATALOG The file can then be processed using a spreadsheet program The file name will be formatted as DLYear_Month_Day_ Hour Minute_Second CSV To send data to a Printer see section 5 2 6 2 on page 32 Plug in the USB device Figure 2 on page 13 Press Recall Data from the Main Menu Press Options and then the PC amp Printer icon Select the data to send to the memory stick and press OK Note The number in parenthesis is the total number of data sets assigned to this selection To send measurement data to a PC The optional HACH Data Trans software must be installed on the PC see section 5 2 6 3 on page 32 37 Standard Operations 5 3 1 4 Delete stored data from the data log Feca Lett AA E aoo 1 Delete Data Press Recall Data in the Main Menu 2 Press Data Log gt Options gt Delete Ge Single point C Filtered data 4 3 Highlight Single Point or Filtered data or All data and press OK to confirm C All data 52 ey D OK E 5 3 2 Store recall send and delete data from wavelength scan and time course The instrument can store 20 Wavelength Scans and 20 Time Course Data sets The data can be stored manually at the user s discretion after viewing the data Note The number in parentheses is the total number of data sets assigned to this selection 5 3 2 1 Data storage from wavelength scan or time course wW length EROS fe
87. phanumeric keypad ABC abc Alphabetic When entering alphabetic characters ex user entered units this key allows to toggle between upper and lower case letters Symbols Punctuation symbols and numerical sub and superscripts may be entered Clear Entry Clear the entry SE one position This deletes the character previously entered in the new Right Arrow deco next space in an entry when two adjacent characters occur on the 23 Standard Operations 5 1 3 Main Menu Main Menu A variety of modes may be selected from the Main Menu The O Stored Ti Barcode following table briefly describes each menu option Programs Programs User Favorite System Instrument Checks Setup Table 2 Main Menu options Stored programs are pre programmed methods that make use of HACH reagents and vial tests The DR 3800 Procedures Manual contains illustrated step by step procedures for analyzes using HACH programs Stored Programs Barcode Programs User programs make made to measure analysis possible User Programs Users can program methods they have developed themselves Existing HACH methods can be stored as user programs Favorite Programs List of methods tests created by the user to suit his own requirements Single wavelength measurements are Absorbance measurements The light absorbed by the sample is measured in absorbance units Single Wavelength Transmittance measurements The percentage of the light that passes throu
88. ple vial is placed in cell compartment 1 Figure 10 the corresponding measurement program is automatically activated in the Main Menu e Otherwise press Barcode Programs in the Main Menu and insert the blank or sample cuvette vial depending on the working procedure in cell compartment 1 The measurement is started automatically and the results are displayed To evaluate other cuvette vial tests and other parameters insert the prepared cuvette vial into the cell compartment and read the result Note The control bar displayed on the right of the screen shows the relationship of the measurement result to the measuring range The black bar shows the measured result independently of any dilution factor that was entered 5 5 2 Select the measuring range Stored Programs 326 Magnesium I 10 0 mgl 326 Magnesium II Main Select by Start Menu Number Permanent Start Standard Operations some tests can be used for different measuring ranges After the sample cuvette vial has been inserted a list of the different measuring ranges is displayed Select the required measuring range by highlighting the appropriate line Press Start Permanent if this measuring range is to apply to all subsequent measurements Changing the standard setting In the result display press Options gt More gt Permanent On The key will change to Permanent Off 5 5 3 Select the chemical evaluation form Optic Chemical Form 3 POA
89. r Programs to the favorites list or the favorite programs see section 6 1 4 on page 75 and section 5 4 10 on page 50 1 Press Favorite Programs in the Main Menu The Favorite Programs list will appear 2 Highlight the selection by pressing it or press Select by Number to search for the program by number Note Use the scroll bar to scroll through the list quickly Use the alphanumeric keypad to enter the test number program number and press OK to confirm 3 Press Start 1 Press Favorite Programs in the Main Menu The Favorite Programs list will appear 2 Highlight the selection by pressing it or press Select by Number to search for the program by number Note Use the scroll bar to scroll through the list quickly Use the alphanumeric keypad to enter the test number program number and press OK to confirm 3 Press Remove Program and press OK to confirm Note If a Favorite Programs is deleted it will stay in the User Programs or Stored Programs Note If the stored program is deleted in User Programs it will also be deleted in Favorite Programs 77 Advanced Operations 6 3 Standard Addition monitoring checking results 78 The accuracy of measured values their correspondence with the actual concentration of the analyte in the sample and their precision correspondence of the measurement results obtained from several samples containing the same concentration of the test analyte can be determined
90. ram a user method All input steps and their significance and options are explained in the following sections 1 Select New in the Program Options menu 57 Advanced Operations User Program Number 950 999 7 Program Number Specific test number with which the program can subsequently be called up from the selection list in the User Programs menu or the Favorites menu 2 Use the alphanumeric keypad to enter a program number between 950 and 999 The lowest available number appears automatically 3 Press OK Note If the program number is already assigned to another user program a message appears asking whether the existing program should be replaced Press OK to overwrite the existing program aa Program Name Program Name 4 Use the alphanumeric keypad to enter a program name The name can be a maximum of 28 characters long 5 Press Back to go back to the previous program point or press Next to continue with the input of the program data Time User Secs en Program Type Program Type EE 6 Select the required option Table 7 and press Next C Multi Wavelength 7 Ifthe Single Wavelength section 6 1 1 1 on page 59 or Multi Wavelength section 6 1 1 2 on page 60 is selected define the units wavelength absorbance formula wavelength Ax concentration factor Kx concentration resolution chemical form and calibration equation CANCE GEI ana More information on Free Programming parameters se
91. ready stored Press OK to overwrite the stored data Option 2 1 Press Wavelength Scan or Time Course and then Options gt More gt Send Data to send the data to a USB memory stick or to a printer e When a printer is connected select how to send the data to the printer graph table or both graph and table e When a USB memory stick is connected the files will be automatically sent as CSV files Comma Separated Value to a file WLData Wavelength Scan Data or TCData Time Course Data The file name will be formatted as ScanData_Year Month_Day Hour Minute _Second CSV Wavelength Scan Data or The file name will be formatted as TCYear_Month_Day_Hour_Minute_Second CSV Time Course Data Standard Operations For further processing use a spreadsheet program 5 3 2 4 Delete stored data from wavelength scan or time course 15 SEP 06 Scan 7 18 SEP 06 Scan 16 SEP 06 Scan D 18 SEP 06 Scan 10 18 SEP 06 Scan 11 14 20 33 400 600 nm 001 nm 09 19 40 400 600 nm 4 001 nm 00 26 44 400 600 nm 4 001 nm 09 29 14 400 600 nm A 001 nm 10 01 32 400 600 nm A 001 nm Wavelength Scan el Options Press Recall Data from the Main Menu and then Wavelength Scan or Time Course or Options gt More gt Recall Data A listing of the stored data is displayed Highlight any data to delete Press Delete in the Options menu and press OK to confirm 41 Standard Operations 5 4 Stored Pro
92. rentheses or the calculation of a logarithm is mathematically permissible in the defined formula this also applies to the basic mathematical operations The following mathematical operations are available Addition Subtraction Division A Exponent x Multiplication n Ln Natural logarithm Log Common logarithm User Pere oes Press gt lt to include logic statements links conditions in Conditional operator the formula The following functions are available Equal to lt Less than gt Greater than lt Less than or equal to gt Greater than or equal to IF THEN ELSE When the evaluation formula C1 has been entered completely press OK to confirm Press OK again to return to the Formula display When the formula C1 has been entered and confirmed the parameter name upper and lower limits of the measuring range and display result yes no can be entered A Entering the next calibration formula C2 or Cn 1 Highlight C2 Off and press Edit 2 Select C2 Off again and press Edit The display switches to C2 On 3 Highlight the next line C2 to define the formula and press Edit In addition to the keys described in Table 10 only one other function is available 73 Advanced Operations ra Select Conc 1 1 Cancel AA AN 6 1 2 4 Enter variables User Ses Variables Fi 5 0360 Select Conc key If an already defined formula in thi
93. riginal curve size can be restored by pressing the zoom icon again Arrow keys 94 6 6 3 Work with reference scans Select Reference Scan LaL HEES 400 600 nm 001 nm 01 SEP 06 09 44 43 Scan 2 400 600 nm 001 nm 11 15 54 400 600 nm 4 001 mm Ee Ee 400 600 nm 001 nm 14 06 56 Scan 5 400 600 nm 001 nm Reference Highlight Highlight Off Reference Data Reference Scan 400 nm 400 A 500 nm Difference 0 447 Options Advanced Operations There are two options to work with Reference Scans First option 1 Press Reference Off in the Options menu to select another scan to display on the same screen with the current scan Highlight the required scan number and press Highlight Reference Note After selecting a reference scan the Reference Off key in the Options menu turns into Reference On Note Only scans that have the same wavelength range and step can be displayed using the overlay option The reference curve is shown in orange The absorbance or transmittance value and the associated wavelength are highlighted in orange Note A black and an orange box are shown in the left corner of the display The orange box relates to the reference scan and the black one relates to the current wavelength scan To complete the wavelength scan reading see section 6 6 2 on page 93 e The newly plotted wavelength scan curve is shown in black e The absorbance or transmittance value an
94. s case C1 is to be taken into account in the formula for C2 press Select conc Enter the number of the formula e g 1 for C1 and press OK to confirm Cn can now be linked with a mathematical operation Note The Cn concentrations that are to be calculated are numbered in sequence C1 C2 C3 etc Note When the first Cn formula has been defined the Formula list is automatically extended by Cn 1 1 Highlight the Variables line in the data overview and press Edit 2 Select the variable to be edited press Edit and use the alphanumeric keypad to enter the data specified in the working procedure for F1 F2 11 U1 etc Press OK to confirm each entry Abbreviation of Variables F1 Factor 1 F2 Factor 2 11 Wavelength 1 U1 Conversion Factor 1 for the first chemical form U2 Conversion Factor 2 for the further chemical form etc 6 1 2 5 Save a free programming user program 74 1 Press Store to save the entered data The data can be stored under any data point Measurement sequence Formula Timer etc 2 Press the PC amp Printer icon to send the program data to a printer or to a USB memory stick connect the USB memory stick to the USB interface first Note The test data will be formatted in the preinstalled PrgData folder as a csv and as a lst file 3 To transfer the program data from one instrument to another create a new folder on the USB memory stick named dbhlc and copy the Ist files into th
95. s in the Main Menu Stored HI Barcode Programs Programs User Favorite System Instrument Checks Setup System Checks The System Checks menu contains instrument information and Instrument Instrument various performance tests Information Update Optical Checks Output Checks Lamp History Factory Service 5 ice Ti Instrument ervice Time Backup de Main Menu 6 8 1 Instrument information 5 sip eh za i e H 4 Instrument Information Press Instrument Information in the System Checks menu DR 3800 i 2 The model serial number and software version are displayed S N 1175048 Instrument Version 0 23 35 2 4 Program Yersions HACH 26 HACH LANGE 530 ox 101 Advanced Operations 6 8 2 Upgrade of the instrument software CU Ce mh sa als Instrument Update Please insert an Instrument Update Module into a USB Port or connect to a computer that is running the update program lo coe oe 6 8 3 Optical checks Optical Checks Wavelength Nome Check Check Drift Stray Light Check Check 102 Absorbance Check Yerification Kit To obtain the software for the update from the Internet at www hach com 1 Goto http www hach com 2 On the DR 3800 product page click Lab System Software Software Update Downloads under Downloads 3 Locate the appropriate download and follow the prompts for saving the file s to the USB memory stick 4 Onthe DR 380
96. sample blank value to be determined as turbidity and color are dealt with during the test procedure Using the data provided in the barcode the instrument automatically sets the measurement wavelength and factors If a discrepancy is detected between the barcode data and the stored data or a new test is identified the instrument requests an update see section 5 5 7 55 Standard Operations 5 5 7 Upgrade of the instrument software CU Ce mh sa als Instrument Update Please insert an Instrument Update Module into a USB Port or connect to a computer that is running the update program ga To obtain the software for the update from the Internet at www hach com 6 Go to http www hach com On the DR 3800 product page click Lab System Software Software Update Downloads under Downloads Locate the appropriate download and follow the prompts for saving the file s to the USB memory stick On the DR 3800 press Instrument Update in the System Checks menu Connect the USB stick to the USB interface on the DR 3800 see section 3 4 on page 13 Press OK The link is established automatically and the software is updated Press OK to return to the System Checks menu Note When the instrument software has been updated a prompt to restart the instrument is displayed 56 Section6 Advanced Operations 6 1 User Programs User programs provide the opportunity to complete made to measure analysis
97. sorbance at wavelength 1 Az is the absorbance at wavelength 2 and so on K is the factor at wavelength 1 K is the factor at wavelength 2 and so on If a subtraction has to be completed the factors can be entered with a minus sign Wavelength 1 Press a A key and use the alphanumeric keypad to enter a wavelength Press another A key and enter the next wavelength If necessary repeat until all the wavelengths for the formula have been entered The wavelengths must be in the range from 320 1100 nm Press OK Concentration Factor Kx Multiplication factor for converting absorbance values into concentration values Press a factor key and use the alphanumeric keypad to enter a factor If the formula includes more than one factor press another factor key and enter another factor and repeat until all the factors have been entered Press OK When all the relevant data have been entered press Next Note Up to five digits can be entered including a maximum of 4 decimal places to the right of the decimal Concentration resolution number of decimal places Select the required number of decimal places from the displayed list and press Next 61 Advanced Operations weg Chemical Form Chemical form Enter the chemical formula used in the display to represent the analysis parameter Use the alphanumeric keypad to enter the chemical form and press Next to enter Calibration settings 6 1 1 3 Calibration settings for singl
98. ss Integral Off in the Options menu 2 Highlight On to show the Integral To find the integral of other wavelength ranges change the wavelength range and scan again 3 Press OK to confirm Optic Scale Units Units DG Abs Scale CG Auto C Manual 0 300 Abs ru 3 500 Abs Cancel OK e 6 6 2 Wavelength scan reading Wavelength Scan 400 zeroing Main Cancel Menu Options Advanced Operations 4 Press Return to return to the scan mode Note The Integral is shown instead of the date on the display Note For the next scan measurement the setting for the Integral will be On Scale units 1 Press Scale 8 Units 2 Highlight the required units Abs or T 3 Highlight Auto or Manual scaling on the graph s y axis Note If manual scaling is selected use the alphanumeric keypad to set the limits Y min and Ymax The graph is adjusted to display only the values in the selected range If automatic scaling is selected the instrument sets the limits automatically so that the total range can be displayed 4 Press OK 5 Press Return to return to the scan mode After the scanning parameters have been selected the baseline must be scanned Changing any of the scanning parameters requires a new baseline scan When the baseline has been scanned the instrument is ready to scan one or more samples 1 Press Wavelength Scan in the Main Menu 2 Insert the blank cuvette cell i
99. step Note Scan recordings of high resolution data take a longer time than recordings of low resolution data Selecting a larger step allows the instrument to scan faster but decreases the resolution of the collected data 91 Advanced Operations 0 515 0 530 0 530 0 514 0 564 0 634 Integral 323 54 Optic a Track Valley Peak Walley 2 Walley h Options Cursor Mode GG Peak alley Cancel Integral Cancel 92 5 According to the selected wavelength range select the interval from the actively displayed wavelength steps In summary maximally 780 measuring steps can be accomplished during a Scan Note The maximum wavelength adjusts automatically if the difference between the maximum and minimum wavelength is not a multiple of the interval 6 Press OK to return to the scan mode Selected parameters are displayed along the graph s x axis Select view displaying table 1 Press Select View in the Options menu after a reading is taken 2 A table with the results is displayed 3 To return to the graph press Options and then View Graph Cursor mode 1 Press Cursor Mode Track in the Options menu 2 The selection for this menu item determines what data are displayed in the table Highlight Track or Peak Valley 3 Press OK to confirm 4 Press Return to return to the scan mode Integral The Integral applies to the whole wavelength range of the scan 1 Pre
100. t allows entry of up to 5 significant digits with a maximum of 4 significant digits after the decimal point Concentration factor 1 Press Concentration Factor Off in the Options menu Press On to highlight this feature Press the Factor key to enter the factor by which absorbance readings are to be multiplied Press the Unit key to select the units for concentration measurements or to create a new unit Press OK to confirm Concentration resolution 1 2 Press Concentration Resolution in the Options menu Select the resolution and press OK to confirm Advanced Operations 6 5 2 Complete a measurement in the multi wavelength mode SE e ee 1 Insert the blank cuvette cell into the cuvette sample cell holder Press Zero 2 D 5 5 Note The Read key does not become active until the zero F ean measurement has been completed 2 561 O 214 2 Insert the sample cuvette cell into the cuvette sample cell holder Press Read 20 5EF 2006 11 05 04 3 For data storage see section 5 3 1 on page 36 Ah Options 89 Advanced Operations 6 6 Wavelength Scan mode recording of absorbance and transmittance spectrums In the wavelength scan mode the absorbance of the light in a solution over a defined wavelength spectrum is measured The measurement results can be displayed as a curve as percent transmittance T or as Absorbance Abs The collected data can be printed as a table or a curve Th
101. t data are automatically deleted allowing the new data to be stored 5 3 1 2 Recall stored data from the data log to Re 14 al 25 0 000 mod Alf 15 SEP 06 14 51 56 0 114 mod Al 18 SEP 06 09 16 39 0 110 mg L Alf 15 SEP 06 10 59 06 0 118 mg L AIS 15 SEP 06 11 01 14 0 000 mod Al Main Filter Menu Off 36 View Details Data Log 52 h Options 1 Press Recall Data in the Main Menu 2 Press Data Log A listing of the stored data is displayed 3 Press Filter On Off Filter Ce On Sample ID Operator ID WwW 05 lt All gt E Start Date Parameter 7 lt All gt lt All gt 5 Cancel O 15 SEP 06 09 15 04 3 93 mall POy47 P 15 SEP 06 09 15 40 3 95 mg l POy7 P 15 SEP 06 10 03 24 Ge i a i 2 mg L AlI 15 SEP 06 10 08 00 0 000 mg L Al Main Filter Wiel Menu On Details K Data Log 4 WW OS 02 a Wu 06 04 UU 05 05 al Options 5 3 1 3 Send data from the data log Reca a E RH Send Data Ge Single point C Filtered data 4 C Alldata 52 ey D OK E Standard Operations The function Filter Settings is used to search for specific items Highlight On to turn on the filters to select data by e Sample ID e Operator ID e Start Date e Parameter or any combination of the four Press OK to confirm the selection The chosen items are listed Press View Details to get more information Data are sent from the data log as CSV Comma Se
102. tandard addition volume O mL stands for a sample containing no added standard The second column shows the reading of the samples with and without added standard The third column shows the detection rate of the added standard Note The black highlighted line is active 8 10 The reading of the sample in the instrument without added standard is automatically shown under 0 mL Prepare the standard addition solution as described in the procedure Use the arrow keys to select the first standard addition volume in the table and insert the cuvette with the corresponding volume of added standard into the cell compartment Press Read Repeat the procedure from point 8 with all the other standard addition solutions 81 Advanced Operations Standard Additions Approx Conc 0 228 mg Lal 0 431 mg L Curve Fit r 0 9993 82 11 12 After all the standard addition solutions have been measured press Graph The regression line through the standard addition data points is displayed The correlation coefficient r2 indicates how close the data points are to the line If the correlation coefficient 1 the curve is linear The concentration shown above the curve is the estimated concentration of the sample without the added standard Note In the curve menu the name on the Curve key switches to Table Press Table to display all the data in the table again Press Ideal line to display the rela
103. tation of Remedies The remedies of repair replacement or refund of purchase price as stated above are the exclusive remedies for the breach of this warranty On the basis of strict liability or under any other legal theory in no event shall Hach Company be liable for any incidental or consequential damages of any kind for breach of warranty or negligence 127 Limited Warranty 128 A eeler nen et 106 Absorbance Formula 86 87 Alphanumeric keypad 23 if Lelleg e EE 28 B Barcode Programs ccoococcc 24 51 52 53 54 55 o EE 54 Sege WEE 42 54 C Calibration formula oocoococcoccocccccononcononononenccnanoss 11 Calibration Settings oocococccoccoconoconocanocanonnos 62 Ee Ee WEE 15 ChemiCal FOTO ainia caia eege dese 43 47 54 CHEMICAL TOM isc ida 53 Cleaning ISP aii di 115 Spectrophotometer oocccccccccnocococonncoononanonannns 115 COlOF SCIGCHON calidad 35 Concentration Factor ccocccccnccnccno oo 83 84 86 88 Concentration Resolution 83 84 86 88 Continuous Reading cocccoccocccncccoccnoncncncoronanonons 43 45 Contool Dar gege ee E eaten 52 Cursor Mode Ask KRANKER 90 92 CUv ettes Cell uni ae re 115 D RE HEEN 36 WE Late W 36 38 Kiel ele EE 36 38 SES 36 38 SIONO ee ee 36 38 RI Ne uranio la idad 36 Data Sirera 38 Date and Time cocococcnccnconcnconcnconcnconcnnnnnonannononnnnnnnos 27 DION Factor asirio ia 43 46 54 ISP tata dal
104. te USB cables must not be longer than 3 meters 10 feet These USB interfaces enable data and graphics to be output to a Printer and a PC and upgrade of instrument software see section 6 8 2 on page 102 Figure 2 Interfaces 1 USB type A 3 Plug in power supply 2 USB type B 4 On Off switch 13 Installation 3 5 Cell compartments cuvette sample cell adapters light shield and Adapter Box 3 5 1 Cell compartments and adapters The DR 3800 has two cell compartments 3 Only one cuvette sample cell type at a time can be used for a measurement Cell compartment 1 e 13 mm and 16 mm round cuvettes vials Note Cell compartment 1 contains a barcode reader for cuvettes vials Cell compartment 2 Cell compartment 2 uses adapters to accommodate different cuvette sample cell types e 1 inch square or 50 mm rectangular cuvettes cells can be inserted directly into the cell compartment without using an adapter e Adapter A 10 mm square cuvettes cells e Adapter B Pour Thru cells refer to the instruction sheet supplied with the Pour Thru cell and multi path cuvettes cells Note Pour Thru Cell must be used with Adapter B not Adapter C e Adapter C 1 inch round cells and AccuVac Ampules Note 1 inch round cells and AccuVac Ampules must be used with Adapter C not Adapter B Figure 3 Cell compartments 1 Cell compartment 1 2 Cell compartment 2 14 Installation TY Figure 4 Cuvette sample cell adapters
105. the glass as substances on the skin can bake onto the lamp bulb and thus accelerate the aging process of the lamp Plug a new halogen lamp to the panel Insert the halogen lamp with the half rounded part pointing up Press the plug with slight pressure into the direction of the halogen lamp and push the pressure spring up so that it will engage Carefully insert the lamp fitting again Fold the fan again so that it engages Use a screwdriver to screw the back cover to the instrument Plug in the power supply Reset the Lamp History see section 6 8 5 on page 110 117 Maintenance 7 3 Filter pad maintenance 7 3 1 Filter pad replacement 118 To determine when the filter mat needs to be replaced inspect the filter mat every 3 6 months in a relatively dust free environment this interval can be longer 1 Remove any cuvettes and cuvette sample cell adapter from the cell compartment Turn the instrument off Unplug the power cord Lift the instrument and check the color of the filter mat Replace the filter mat if is dark gray or black Carefully turn the instrument over and place it on a soft surface Use a screwdriver standard or cross head to open the filter grid Figure 11 item 1 Lift the filter grid Figure 11 item 1 Maintenance 8 Remove the old filter pad Figure 11 item 3 and replace it with a new one 9 Screw the grid back in place 10 Carefully stand the instrument upright
106. the measurement used a standard concentration that is different from the one displayed in the box press the box on the right to enter a different standard value and enter the new value Press OK to confirm 5 Press Adjust to enable the Standard Adjust The Standard Adjust icon will appear Note The adjustment must be within certain limits which vary with each program The allowable percentage is shown after Adjustment Note When a Standard Adjust is in effect the Standard Adjust icon will appear on the display Some Stored Programs allow a variety of chemical forms to be selected Press the unit e g mg L or the chemical representation of the evaluation form e g AT A list of available evaluation forms is displayed Select the required form by pressing the corresponding entry in the list Note To exit from the program the evaluation form reverts to the standard setting An alternative way of changing the standard setting 47 Standard Operations Chemical Form Save as Default Press Options gt More gt Chemical Form Select the Chemical Form Note The stoichiometric conversion of the measurement result is carried out automatically Note The selected Chemical Form will appear on the display Test results will be calculated and displayed in this chemical form 5 4 7 1 Change of the default setting of the chemical form 5 4 8 Run a reagent blank 48 1 4 Insert the sample cuvette cell or bla
107. tionship between the added standard solutions and the ideal line detection rate 100 Advanced Operations 6 4 Single Wavelength absorbance concentration and transmittance measurements The Single Wavelength mode can be used in three ways For sample measurements at a single wavelength the instrument can be programmed to measure the absorbance transmittance or concentration of the analyte Absorbance measures the amount of light absorbed by the sample in units of Absorbance transmittance measures the percentage of the original light that passes through the sample and reaches the detector Turn the concentration factor on to select a specific multiplier for converting absorbance readings to concentration In a graph of concentration versus the absorbance the concentration factor is the slope of the line 6 4 1 Set up single wavelength mode Press Single Wavelength in the Main Menu Press Options for Parameter Setup Single Wavelength VIS Ki Options Concentration Concentration Factor Off Resolution 0 01 Reading Mode Save as Single User Program Trans foe See E ar h Instrument Options Return Setup Table 11 Single wavelength setup options A A AAA For further Options With the Store On setting all measurement data are stored automatically With the Store Off Store Off On setting no measurement data are stored Trans Abs To switch to transmittance concentration or absorbance readings
108. to confirm Sending Data is displayed until the data have been printed The optional HACH Data Trans software must be installed on the PC for the subsequent to process for measurement data 1 Press PC Printer in the Instrument Setup 2 Select PC 3 Press Setup to display the PC Setup screen For further installation instructions refer to the HACH Data Trans user manual ALA am AAA PC Setup Auto Send Ce On Remote Si A nn 5 2 6 4 Help Guide 351 N Nitrate LR Wise 507 nm Elter 5 2 7 Password Instr A Ch zabksansca Password Security Ce off Set Password Security List OK Standard Operations 4 Select Auto Send On to send all measured data automatically to the PC Note If Auto Send Off is selected the PC Printer icon must be pressed in order to send data to the PC Note The remote function is only for monitoring the data transfer The Help Guide is a step by step guide for the DR 3800 to complete a test method in accordance with the working procedure The Help Guide is an accessory available on a USB memory stick The Help Guide is available for the most used Hach tests Note For more information about available tests methods refer to the documentation supplied with the Help Guide The Password menu contains a variety of security settings to control access to various functions For example prevent unauthorized changes to stored programs or instrument
109. tup Table 12 Multi wavelength setup options continued Call up saved measurement data wavelength scans or time courses see section 5 3 on Recall Data page 36 Instrument Setup Basic data of the instrument see section 5 2 on page 25 A Absorbance formula an eee ee 1 Press Absorbance Formula 2 The formula selected in the top key determines the number of wavelength and coefficent keys that will appear below To 7 SS change the absorbance formula press the top key select a 1 0000 1 0000 IN MN formula from the displayed list and press OK When a new formula is selected the number of variables below changes to Cancel OK match AS The following formulas are available Ke hatha K A KoAn K3A3 ef et E e E ECH KA K2A gt KA K2A2 K3Az3 Ky Ay K2A2 K3Az3 Kg Ag A 4 refers to the absorbance at wavelength 1 A refers to the absorbance at wavelength 2 etc K refers to the coefficient at wavelength 1 K gt refers to the coefficient at wavelength 2 etc Coefficients can be set negative where subtraction is required 87 Advanced Operations Optic 88 Concentration Resolution To change a wavelength press one of the 1x keys Enter the desired wavelength coefficient into the numeric keypad Press OK to confirm To change a coefficient press one of the Kz keys Enter the desired coefficient into the numeric keypad Press OK to confirm Note The instrumen
110. ument see Figure 2 on page 13 These interfaces can be used for exporting data and graphics to a printer updating data and for data communication to a personal computer These interfaces can be used for the connection of a USB stick an external USB keyboard or a USB Barcode handset scanner Note A USB hub may be used to connect several accessories at a time A USB memory stick is used to upgrade data and software see section 6 8 2 on page 102 Important Note A screened USB cable must not be longer than 3 m Table 3 USB connector This USB interface is only intended for the connection DR 3800 PC with installation of the HACH Data Trans Software This USB port can be used to connect a printer a USB memory stick a barcode scanner or a keyboard Inst Ch za oe s PC amp Printer 1 Press PC amp Printer in the Instrument Setup Printer Deskjet 6500 l sica e e A list with information about the connections opens a PC Connected 5 2 6 1 Printer setup REKT e For reasons of compatibility the printer language must be PC amp Printer HP PCL 3 Printer Deskjet 6500 PC Connected 2 Press Printer 3 Press Setup to display the Printer Setup screen 30 Inst pos TT Printer Setup Resolution 100 dpi 8 Lag TS Printer setup Resolution 100 dpi Auto Send Instr SR es Cte S Resolution Y 100 dpi 150 dpi Cancel OK Standard Operations Printer Setup e Resolutio
111. ure 7 Figure 7 Light Shield in place 1 Light shield 18 Installation 3 6 Adapter Box The inside of the Adapter Box Figure 8 can be used for the storage of the three cuvette sample cell adapters The cuvette sample cell adapter recesses in the Adapter Box are marked with the corresponding letters and arrows of the adapter The arrows indicate the direction of insertion When a cuvette sample cell adapter is in use and removed from the cover The light shield can be stored in the Adapter Box Figure 8 Adapter Box Adapter Box 3 Adapter Box with with inserted cuvette sample cell 2 Adapter Box inside view adapter A in position A The inside of the Adapter Box is intended to house the cuvette sample cell adapters The recesses for holding the cuvette sample cell adapters are marked with the corresponding letters 19 Installation 3 7 Beam path Figure 9 shows the beam path of the DR 3800 Figure 9 Beam path 1 Tungsten lamp 9 Filter wheel 2 Entrance slit 10 Splitter mirror A Heat protection glass 11 Reference element 4 Grating 12 Cell compartment 2 5 13 Lens 6 14 Measurement element 7 i 15 Cell compartment 1 8 20 Section4 Start Up 4 1 Power the instrument on and off 4 2 Language selection Main Language English Deutsch 4 3 Self Check Main DR 3800 Self Check SYSTEM TESTS LAMP TESTS FILTER ADJUSTMENT A CALIBRATION mb VOLTAGE TESTS 1 Plug externa
112. x 36 06 1 225 7784 info hach lange hu www hach lange hu HACH LANGE D O O Fajfarjeva 15 SI 1230 Dom ale Tel 386 0 59 051 000 Fax 386 0 59 051 010 info hach lange si www hach lange si Repair Service in the United States HACH Company Ames Service 100 Dayton Avenue Ames lowa 50010 Tel 800 227 4224 U S A only Fax 515 232 3835 HACH LANGE LTD Pacific Way Salford GB Manchester M50 1DL Tel 44 0 161 872 14 87 Fax 44 0 161 848 73 24 info hach lange co uk www hach lange co uk HACH LANGE FRANCE S A S 33 Rue du Ballon F 93165 Noisy Le Grand T l 33 0 1 48 15 68 70 Fax 33 0 1 48 15 80 00 info hach lange fr www hach lange fr HACH LANGE AB Vinthundsvagen 159A SE 128 62 Sk ndal Tel 46 0 8 7 98 05 00 Fax 46 0 8 7 98 05 30 info hach lange se www hach lange se HACH LANGE SP ZO O ul Opolska 143 a PL 52 013 Wroctaw Tel 48 0 71 342 10 83 Fax 48 0 71 342 10 79 info hach lange pl www hach lange pl HACH LANGE S R L Str Leonida nr 13 Sector 2 RO 020555 Bucuresti Tel 40 0 21 201 92 43 Fax 40 0 21 201 92 43 info hach lange ro www hach lange ro HACH LANGE E N E AuAidoc 27 GR 115 27 A8nva TnA 30 210 7777038 Fax 30 210 7777976 info hach lange gr www hach lange gr Repair Service in Canada Hach Sales amp Service Canada Ltd 1313 Border Street Unit 34 Winnipeg Manitoba R3H 0X4 Tel 800 665 7635 Canada only Tel 204 632 5598
113. y at all times Splashes or spills on and in the instrument should be cleaned up immediately see section 7 1 on page 115 Important Note Protect the instrument from temperature extremes including heaters direct sunlight and other heat sources 11 Installation 3 3 Power connections Install the correct adapter plug on the supplied external power supply Figure 1 by sliding the adapter on until it clicks into position Correctly mounted both housing of power supply and plug are in line Plug the external power supply cord into the connector on the back panel of the instrument then plug the supply into a power outlet 100 240 V 50 60 Hz Press the power switch on the back of the instrument to initialize power Figure 2 on page 13 Figure 1 Power adapter 1 Power supply with EU adapter plug installed 3 USA adapter plug 2 UK adapter plug 4 AUS China adapter plug 12 Installation 3 4 Connection The DR 3800 has two USB interfaces as a standard feature located on the back of the instrument Figure 2 The USB Type A interface is used for communications with a printer USB memory stick barcode scanner or keyboard A USB memory stick is used to update instrument software The USB Type B interface is used for communications with a PC The optional Hach Data Trans software see Section 9 on page 123 must be installed on the PC for this use A USB hub may be used to connect several accessories at a time No
114. zero measurement is to be completed Press OK and confirm the input by pressing OK again 3 If zero measurements are to be carried out at a number of wavelengths repeat the above two steps for each wavelength Note The entered measurement sequence is displayed 69 Advanced Operations O weem Timer mm ss em Measurement Process Zeroing Process Timer ce Cancel User Sess Ue Measurement Process 70 Process Timer key 1 Press the Process Timer key to enter any waiting reaction or handling times that have to be taken into account Use the alphanumeric keypad to enter the time Press OK and confirm the input by pressing OK again Note This time is integrated into the measurement process Note The entered measurement sequence is displayed R key Reading 1 Press the R key to program a measurement of the substance that is to be analyzed Confirm with OK 2 Press New and then Reading and use the alphanumeric keypad to enter the wavelength at which the measurement is to be completed Press OK and confirm the input by pressing OK again 3 If measurements are to be completed at a number of wavelengths repeat the above two steps for each wavelength Note The entered measurement sequence is displayed key Elements of the measurement sequence that are to be repeated are placed in brackets The left bracket marks the start of the sequence that is to be repeated and the r

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