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HLf Series Syringe Pump User Manual

1. ACCESSORY 6 Panhead screws 2 DIGITAL DIGITAL GROUND OUTPUT Wire pair conversion table for A B Pump Pair and C D Pump Pair A B Pump Digital C D Pump Digital Wire Pair Output ler Wire Pair Output wd B3 B3 415 B3 D7 415 B4 B4 15 B4 D8 15 Figure 5 2 Air valve installation for 500HL 5 4 Pre swaged 1 4 male connectorr Check Valve 4 Panhead screws 4 amp amp ag Outlet 248 1 4 Valco Nut S 1 4 Valco Ferrule Pressure Transducer Cap 6 Panhead crews 2 Wire pair conversion table for A B Pump Pair and C D Pump Pair Inlet customer reservoir DIGITAL 15 GROUND HL Series Syringe Pumps Section 5 Continuous Flow Introduction Installation amp Operation Figure 5 3 Air valve installation for 1000HLrpump A B Pum Digital C D Pum Digital Wire Pair EP 15 Wires Wire Pair Gum 15 Wires A1 Al 15 Al C5 115 A2 A2 115 A2 C6 115 B3 B3 115 B3 D7 115 B4 B4 15 B4 D8 15 5 5 HL Series Syringe Pumps Section 5 Continuous Flow Introduction Installation amp Operation
2. CONTROLLER CONTROLLER CONTROL COMPUTER N 1490 BAD GI Ll cy a c Ww Go L E Am jo Uu ER ar ES T gt RE AE XE t A PUMP A PUMP T case 68 1020 198 E 7 Figure 7 2 Serial network connection example Dual connection 7 4 3 Three or More If additional instruments are to be connected in series use the Controllers Compiler daisy chain cable 68 1020 180 This cable is attached to the required remaining connector on the Y cable Figure 7 2 and then to the RS 232 C connectors on the rear panels of the additional con trollers 74 7 5 Serial Control Check List 7 6 Controller Setup 7 6 1 Restore Defaults if desired 7 6 2 Change Defaults HL Series Syringe Pumps Section 7 Serial Interface In order to build and operate a custom program for Isco syringe pumps you must have the following 1 Ao a pm MN 8 Sample program Isco LabView toolkit LabView Compiler purchased separately Connection cable s 1 controller cable 480 7996 00 2 controllers cable 68 1020 198 3 to 7 controllers cables 68 1020 198 and 68 1020 180 Source code serial commands in computer language for DASNET conversion DASNET serial driver The toolkit comes with directories for two different versions of LabView Copy the zip files onto your computer The files within each directory are as follows labview8 5 labview5 8 2 CONTROL LLB CONTROL LLB
3. SN A 1 5 1 A 2 A o 1 Q u i SA g T i U DTS 10 TI E i c P Extruder S Circulating Reactor H bath Rock Core Etc 1 1 1 1 1 1 LI o 0 0 NN Mp Figure 2 8 System with temperature control jacket installed 2 5 3 Fluid Temperature and 2 12 Volume Temperature Ranges The jacket is very useful for SFC applications where cylinder cooling facilitates filling with fluids such as liquid CO The jacket can also be used with a circulating temperature controlled bath to keep the fluid inside the pump at a constant temperature This may be necessary when operating at very low flow rates below 500 ul min where temperature fluctuation can cause flow variations Temperature control jackets must be pre installed at the fac tory The fluid circulation and heating cooling reservoir connected to the temperature control jacket are user supplied Water baths gener ally come with a built in pump and are available in a wide variety of temperature ranges and fluid capacities from many lab equipment suppliers such as Cole Parmer or Thomas Scientific The model 1162 120V refrigerated circulating bath from Sensotec is an example of a bath for applications such as super critical CO The jacket itself is rated from 30 to 100 C However bath tem peratures are also determined by the type of seals used in the syringe pump The standard pump seals are rated up to
4. Jl Jl B Jl Gradient Duration Range 0000 1 9999 0 minutes Final B ge Range 00 00 100 0 L Initial B Range 00 00 100 0 Gradient Total Flow Rate Units milliliters minute Range 000 0000 Maximum Flow Rate Step Number 01 File 01 99 Step Numbers 02 through 99 LGUL F xx S xx IBY xx xx FBY XX XX D XXXX X i E Gradient Duration Final B Range 0000 1 9999 0 minutes Range 00 00 100 0 Initial B Range 00 00 100 0 Step Number 02 99 File 01 99 Figure 7 8 Gradient Download Commands Two pump flow gradient 7 25 HL Series Syringe Pumps Section 7 Serial Interface Step Number 01 LGUL F xx S 01 FL xxx XXXX I Xx XX F xx XX D XXXX X Gradient Duration Range 0000 1 9999 0 minutes Final Gradient Percentage Range 00 00 100 0 Initial Gradient Percentage Range 00 00 100 0 Gradient Total Flow Rate Units milliliters minute Range 000 0000 Maximum Flow Rate Step Number 01 File 01 99 Step Numbers 02 through 99 LGUL F xx S xx I xXX XX F xx Xx D XXXX X EL pps E Ep Gradient Duration Range 0000 1 9999 0 minutes Final Gradient Percentage Range 00 00 100 0 Initial Gradient Percentage Range 00 00 100 0 Step Number 02 99 F
5. 0 0c ccc tee eee vrir aar 5 10 5 44 To RUN o Stop edle er Oed Gs 5 11 5 4 5 Tips for Running Continuous Flow ooooooooooomoomoo 5 11 Section 6 Modbus Configuration 6 1 Overview A edes Leia eat KANG 6 1 6 13 Modbus RTU N Los ore eret ee oe qe DRE sen Sie 6 1 6 1 2 Modbus TCP IP p 2 loce esos ee ee aa eg Rd ran 6 2 6 1 3 Modbus Configuration Options 0 0 cece een eens 6 2 Section 7 Serial Interface TI Overview sa S0 bos ets Sei eee cht tort 7 1 7 2 Network Control and Communication rsvrvvr rar rar raven rr rann 7 2 1 3 USB Interface Gad a iege edle Sestriere SANG 7 3 7 4 Cabling for Serial Control 0 0 e eens 7 3 7 4 1 One Controller 6 2 scavenge tias 7 3 7 4 2 Two Controllers Compiler required 0 c eects 7 4 7 4 3 Three or More Controllers Compiler required o oooooooo o 7 4 7 5 Serial Control Check List 1 2 0 0 en 7 5 7 6 Controller Setup 6 pees eee eil knekk e as ee a 7 5 7 6 1 Restore Defaults if desired 0 0 0c ce eens 7 5 7 6 2 Change Defaults cuco nn ne dk oe ee hardt 7 5 7 6 3 Verify Operation 0 0 0 ccc ee eens 7 6 7 7 User written Software ice anrea renes ene pa e een ene e ene A ai 7 6 TAE DASNET tn a ONE p etti Rae ERES DER 7 6 7 7 2 Universal Driver from Teledyne Isco o ooooooooooomooo 7 7 7 8 Serial Commands for the HLf Series Pump 0 0 0 7 15 7 8 1 Get Status Comma
6. if stremp in R out out 0x00 else sprintf out 3 3X s strlen in in for sum 0 c_ptr c_ptr sum c_ptr sum 0x100 sum amp OxOFF sprintf c_ptr 2 2X sum output converted string send CR to serial port output converted string to serial port end with a CR get response output response flush serial buffer to start again dasnet conversion utility point to output put id first add R to output if just R add space to string add ft char to string add all chars together get check sum insert into string Note Polling is part of the DASNET definition but is not required and is not shown in this example This code was written in TurboC version 4 5 TurboC is a compiler created by Borland Software Corp Austin TX It is no longer actively sold by Borland International 7 9 HL Series Syringe Pumps Section 7 Serial Interface 7 10 There are three types of operation within the network network controller master and slave A computer typically serves as the network controller It supervises all data flow on the network It also polls each unit which initiates data transfer and commands The network controller typically a PC should not be confused with the pump controller The network controller is used in addition to the pump controller The slave unit simply re
7. 3 10 Limits programming options Flow rate limit for pressure control 3 8 8 RAPID PRESS HL Series Syringe Pumps Section 3 Basic Programming and Operation 6 Once all the limits have been set press softkey D PREVI OUS to return to the main menu In addition to setting the upper and lower limits the pump also allows the user to specify whether they want the alarm message displayed an alarm to sound when the limit is reached the pump to shut down when the limit is reached These features are set using the softkeys A C which toggle the feature on and off M Note The Max Press maximum pressure display on and alarm on options cannot be disabled Display When a limit has been exceeded this feature causes the display to automatically flash an OVER or UNDER LIMIT message To turn off this function press softkey A once to toggle to OFF DISPLAY Alarm When a limit has been exceeded this feature causes the pump to automatically beep a warning To turn off this function press softkey B once to toggle to OFF ALARM Shutdown When a limit has been exceeded this feature causes the pump to shut down To turn off this function press softkey C once to toggle to ON SHUTDOWN When the pump is controlling pressure CONSTANT PRESSURE MODE the flow rate is not user controlled and may range up to the maximum flow of the pump In some cases it is desired to limit the rate of pumping during syste
8. ACC CTRL Accessory control Manually operates accessories such as valves ZERO PRESS CLEAR ENTRY Zero pressure Sets pressure display to zero Active only from 750 to 750 psi Clear the last digit entered from the numeric key MENU Accesses software to set operational modes units and other optional parameters DISP HOLD Activates dispense mode refer to Section 3 10 3 Freezes the program clock The unit will continue at the current gradient parameters REFILL Turns on pump drive motor to move piston downward at a rate previously programmed RUN Turns on pump drive motor to move piston upward in a previously pro grammed mode such as CONSTANT FLOW or CONSTANT PRESSURE STOP Stops the drive motor ENTER Enters selected values to memory NUMBER KEYS These keys are used to make menu selections and enter values when setting parameters M Note A more in depth explanation of the keypad is provided in Section 3 Basic Programming and Operation under Section 3 8 Front Panel Keys 1 9 HL Series Syringe Pumps Section 1 Introduction A Figure 1 6 Pump controller rear panel connectors Ay WARNING Explosion Hazard The area must be known to be non hazardous before servicing replacing the unit and before installing or removing I O wiring Do not disconnect equipment unless power had been disconnected and the area is known to be
9. Menu 3 provides programming options for poor fills diagnostics pressure calibration alternative pressure inputs pressure control setting and pressure setpoint tolerance To save all settings and return to the main menu press PRE VIOUS RETURN D 1 POOR FILL ALARM 4 DIFF MODES 2 DIAGNOSTIC MENU 5 PRESS INTEGRAL 3 PRESS CALIBRATION 6 PRESS DEADBAND PREVIOUS Figure 3 6 Menu 3 program selections 3 6 1 Poor Fill Alarm 3 6 2 Diagnostic Menu 3 6 3 Pressure Calibration In constant pressure mode this feature allows you to set a fill point as a percentage of pump volume If this volume percentage is not reached after a refill and re pressurization the system issues an alarm and stops the pump This selection displays an additional menu containing testing options for the system Refer to Section 9 5 for a complete expla nation of how to use the diagnostic menu This is a stored psi value to ensure optimal operation of the pressure transducer On most pumps this value known as the midpoint adjustment can be found on a tag on the transducer cable shown below Standard calibration for the transducer is a two point adjustment at zero and maximum pressure The midpoint adjustment number is the difference between the pressure at half maximum as read by a pressure gauge with a precision of 0 2596 accuracy and the syringe pump transducer output 3 7 HL Series Syringe Pumps Section 3 Basic Programmin
10. Section 3 Basic Programming and Operation 3 1 Introduction 3 2 General Controller Information 3 2 1 Rates Units and Limits 3 2 2 Programming Screens This section will familiarize you with the HL Series pump con troller and describe operating the pump under each of the various modes constant flow constant pressure and refill Pump setup and operation is regulated by the HL Series con troller Operating parameters are entered via the keypad on the front panel of the controller Operating selections are displayed as menu items on the controller screen or are associated with a dedi cated key on the controller keypad Operating modes such as CONST FLOW CONST PRESS DISP and REFILL all have such dedicated keys AN WARNING UL Underwriter Laboratories has certified the HL Series Controller and Pumps on the basisthat explosive chemicals or chemicals that could become explosive under pressure are NOT used The instruments are not explosion proof Use extreme caution when pumping hazardous fluids The following information is intended to familiarize you with controller operation Once you have become familiar with the keypad and the main menu you will find it easy to direct the pumping operations required for your applications If you make an incorrect entry press CLEAR ENTRY to delete your last keystroke If you have entered a programming mode but do not wish to make any changes press ENTER to keep the current sett
11. The pump responds The system is now running and the network controller continues the polling scheme If the controller gives an improper command the units will respond with a problem message indicating the type of error The format of the message is given in section 7 8 of this manual and specifies the commands used for this instrument It is important to follow this format Spaces are ignored anywhere within the message field Commands must be in uppercase letters The network definition allows multiple commands in a message field when delimited by semicolons but the HL Series controller is limited to single commands It will respond with a PROBLEM INVALID COMMAND message 7 8 Serial Commands for the HL Series Pump HL Series Syringe Pumps Section 7 Serial Interface Table 7 5 is a list of the serial commands recognized by the pump These commands are the message part of the DASNET protocol The operand always follows the equals sign The REMOTE command must be sent once before any command that changes the operation of the pump will be accepted When setting a value the serial command will always be fol lowed by an equal sign Table 7 5 Serial Commands Command Description Enter for percentage of modifier Status of the analog voltage input on pin 21 P6 See NOTE 1 Status of the analog voltage input on analog input 3 of the accessory connector See NOTE 1 Status of the analog voltage input on analog
12. oooooooooooororo ee 5 6 5 3 Key functions in the Multi pump Mode 0 00 e cen eens 5 8 6 1 Modbus TCP IP Configuration Options 0 0 cece eens 6 3 6 2 Modbus RTU Configuration Options 0 0 cece ees 6 3 6 3 Supported Modbus Function Codes 0 000 cece eee en 6 3 6 4 Exception Responses 0 cece cee eee ener he 6 3 A AN 6 4 6 6 Holding Registers onies renees eie a ravner rn rev en a e eens 6 8 7 1 RS 232 External control connector serial pin connections 0 ce eee raner eens 7 2 7 2 USB Interface Pin Connections on the USB Ethernet connector 0 cece eee eens 7 3 7 3 Example of BASIC program to demonstrate conversion of pump commands to DASNET frames 000 000 7 8 7 4 Example of C program to demonstrate conversion of pump commands to DASNET frames 0 0000 7 9 XV HL Series Syringe Pumps Table of Contents 7 5 Serial Commands 0 0 rv vaar vr narr rn een 7 6 Error Messages xvi HL Series Syringe Pumps Section I Introduction 1 1 Introduction 1 2 Specifications This manual is intended to instruct the user on pump instal lation The technical specifications for the HL Series Syringe Pumps are detailed in Tables 1 1 through 1 4 M Note This equipment is suitable for use in Class I Division 2 Groups A B C and D T4 hazardous locations and non haz ardous locations only A WARNING Explo
13. 5 4 4 To Run or Stop This menu setting allows the operator to run or stop both pairs of pumps simultaneous PREVIOUS Press 3x to return to run screen MULTI PUMP Four PUMP RUN STOP Figure 5 8 Keystrokes to Run or Stop the pumps M Note Before pressing RUN ensure that ON CONT FLOW is displayed on the screen and that the set flow rate pressure is correct Always verify the valve settings before running a program If a controller is reset or moved to a different power source it will revert back to default settings Active 5 4 5 Tips for Running Liquids Checklist Continuous Flow 1 Degas liquids if appropriate 2 Purge air from the system a Fill both pumps completely by pressing REFILL and selecting each pump to fill b Route the outlet to waste or reservoir and press RUN Press STOP when fluid comes out of the outlet c Open the valves to atmosphere by pressing ACC CTRL then selecting each valve to open d Zero the pressure in each pump by pressing ZERO PRESS and selecting each pump to zero e Connect the outlet tubing and fill each pump once more 3 Reset total volume see Figure 5 5 HL Series Syringe Pumps Section 5 Continuous Flow Introduction Installation amp Operation 5 12 Liquefied Gases Checklist 1 Equilibration When the pumps begin running the system will go through an equilibration phase during which both pumps Open the valves to atmosphere by press
14. HL Series Syringe Pumps Section 3 Basic Programming and Operation 3 10 Operating Modes 3 14 2 Press softkey A MORE 3 Press number 4 MULTI PUMP The multi pump menu will appear 4 Press number 1 for pumps A or B press number 2 for pumps C or D 5 Press number 4 INDEPENDENT The controller will set the pumps to Independent mode Number 4 will blink indicating that INDEPENDENT mode is selected 6 Select the HOLD PRESS or NORMAL mode of operation Press softkey A to toggle between the two modes HOLD PRESS In constant pressure mode after the pump is empty if the outlet pressure rises past the set point the pump will restart and run the system to the set point pressure NORMAL This feature shuts the system down if a pump runs empty in constant pressure mode Once the pumps have been set to this mode they will operate independently from one another Each pump will operate at its defined limit and rate Independent mode is the default setting for the pump When you select a command such as STOP or REFILL the display will prompt you to designate which pump to stop or refill Only the designated pump will stop the other pumps will continue to run 7 Return to the run screen by pressing D three times Then press D SELECT PUMP The display will show each pump s information and allow you to select any pump for program ming changes The pump has three delivery modes and one refill mode When using a si
15. Ka oc KUL TOLG OX TIKOUG t VIKOUG OPOLG N VA EKTAL ELTEL ATO ATOLO TO OTOLO EXEL TPOVPOVUEVOS EPYAOTEL TAVO OTO OPYAVO ALTO gt ppbbb p C men ga u36erHe HOBPena Ha anapaTypaTa wr HapaHABaHe Ha oNepaTopa e HEO XO MMO KIIHEHTA HO Pe Ja Bilafee AHTIIM CKH E3UK U TEXYHUYECKATA TCEpMHHO ITH35 KOATO H3IIO ISYBaHA B OIIHHCAHHETO WIN Ja 6 bIIE o6yuen or mune koero e BEYE PA OTHITO c TAKBB arapar Figyelmeztet s A k sz l k meghib sod s nak valamint a kezel s r l s nek megel z se rdek ben a felhaszn l nak felt tlen l rtenie kell az angol nyelvet ezen bel l a m szaki kifejez seket vagy pedig a haszn latba v telt megel z en a k sz l k kezel s ben m r gyakarlott szem ly ltal t rt n betanit s sz ks ges HL Series Syringe Pumps Warnings Cautions and Notices NOTICE If the pump has been used for pumping a hazardous or potentially lethal material Do not return the pump without contacting the Teledyne Isco Service Department Do not return the pump without first providing written guarantee that it has been decontaminated of hazardous or potentially lethal materials Teledyne Isco reserves the right to refuse shipment if no decontamination assurance has been provided prior to shipment Failure to decontaminate a pump may result in legal action Fluids Certified for Isco HL Pumps Ay WARNING Pumping fluids that are not compatible with the HL Series Pump
16. and G amp serial commands retrieve information from the pump controller Each command returns a text string which can be read as shown in Figures 7 3 and 7 5 7 20 HL Series Syringe Pumps Section 7 Serial Interface R046 37510 00128 01017 06981 05743 00421 00442 00449 11111000 CS Pressure Pump A Pressure Pump B Digital Inputs 1 8 Pressure Pump C 1 High Units psi 5 0 Low Resolution 0 2 psi ALOG1 ALOGS5 refer to Table 7 5 Units volts 5000 Resolution 0 2 millivolt Range 7500 to 58035 1 5000V to 11 6070V Figure 7 3 Get Status String G Command R049 37510 00128 01017 06981 05743 00421 00442 00449 25046 11111000 CS l l m Pressure Pump A Pressure Pump B Digital Inputs 1 8 Pressure Pump C 1 High Units psi 5 0 Low Resolution 0 2 psi ALOG1 ALOG6 refer to Table 7 5 Units volts 5000 Resolution 0 2 millivolt Range 7500 to 58035 7 1 5000V to 11 6070V Figure 7 4 Get Status String GG Command 7 21 HL Series Syringe Pumps Section 7 Serial Interface p Same as Figure 7 3 20B5 37510 00128 01017 06981 05743 00421 00442 0000005921 0010696698 R R 0027500000 0057744222 R R 00449 11111000 0052500000 0016778750 R R 0000005921 37500 00000000 24 Pump B L_ Pump C Control Status Pump A L Local control R Remote control E External control Operati
17. tre affaiblie cela augmentera votre risque de blessure gt gt po AVIS Les liquides qui sont analys s dans cet instrument peuvent tre canc rig nes hasards biologiques inflammables ou radioactifs Si vous devez utiliser ces liquides hasardeux il est tr s recommand que vous le faites l int rieur d un environnement isol concu pour tels liquides Cet environnement isol devrait tre construit selon les r glements f d raux provinciaux et locaux aussi que le plan de votre organisation qui concerne l v nement d un accident avec les mati res hasardeuses En tout cas utilisez toujours l instrumentation d Isco avec prudence et sens commun Foreword This instruction manual is designed to help you gain a thorough understanding of the operation of the equipment Teledyne Isco recommends that you read this manual completely before placing the equipment in service Although Teledyne Isco designs reliability into all equipment there is always the possibility of a malfunction This manual may help in diagnosing and repairing the malfunction If a problem persists call or e mail Teledyne Isco technical support for assistance Simple difficul ties can often be diagnosed over the phone For faster service please have your serial number ready If it is necessary to return the equipment to the factory for service please follow the shipping instructions provided by technical support including the use of t
18. 2R47 256 559 Modulo 512 47 Step 2 472559 256 Step 3 209 256 47 Step 4 Convert 209 to ASCII Hex and put at end of message Step 5 Put a CR 13 at the end of message for end of frame Step 1 22FH 52H 33H 30H 34H 53H 54H 4FH 50H Step 2 FED1H 100H 22FH Step 3 D1H FED1H amp offH HL Series Syringe Pumps Section 7 Serial Interface 7 12 Decimal Format Using NO MODULO Step 4 Convert D1H to ASCII Hex and put at end of message Step 5 Put a CR 0DH at the end of message for end of frame Step 1 559 82 51 48 52 83 84 79 80 Step 2 303 256 559 Step 3 209 303 amp 255 Step 4 Convert 209 into ASCII Hex and put at end of message Step 5 Put a CR 13 at the end of message for end of frame The carriage return CR signifies end of frame The format for frames sent from the unit to the network con troller is as follows acknowledgement message destination length message checksum V CR All the parameters are as previously described except message destination Message destination is the 1 digit identification number of the unit that the message is sent to An example of a typical data exchange is summarized below For illustration we will assume the network consists of a computer serving as a combination network controller and master There will be one slave unit a Model 260D pump Details on the pump messa
19. 5 3 User Supplied Valves 5 6 Users may also choose to supply their own valves The following information is necessary to choose the appropriate valves to work with Teledyne Isco Continuous Flow software Check valves Do not use spring loaded check valves if refilling at atmospheric pressure the pump seals are not designed to draw against a vacuum The balls in Teledyne Isco check valves are closed by gravity Powered valves These are electrically triggered from the pump controller and could be air or electrically actuated Digital outputs 1 4 provide signals for control of four two way valves One wire for each valve is connected to its specific connector on the rear of the controller A second wire for each valve connects to ground The signal provided is an open collector which functions as a switch open for closure of the fluid path A switch closure low signals opening of the fluid path Ay WARNING Connecting devices to energized circuits may cause personal injury or property damage Power must be removed from the pump before connecting external devices Table 5 2 shows the relationship between the digital output pump valve location and the fluid path status for the ACCESS CTRL connections Refer to the accessory control connections on the rear panel of the pump controller Table 5 2 Accessory Control Digital Outputs Digital Pump Valve Fluid Path Status Output Location 1 A Inlet open or closed
20. DASNET DLL DASNET DLL DATA LLB DATA LLB ISCO1 LLB ISCO1 LLB ISCO2 LLB ISCO2 LLB ISCO3 LLB ISCO3 LLB MULTPUMPLLB MULTPUMPLLB PANEL VI PANEL VI README DOC README DOC SERIALIO LLB SERIALIO LLB Once the system is properly connected turn the pump con troller s and computer on Then use the following procedure to place your controller s in serial control mode Press MENU gt MORE A and select SERIAL 1 Factory controller default settings are baud rate 9600 and unit ID 6 To restore defaults press MENU then SYSTEM RESET 5 Resetting a system erases all programs and user settings 1 Press MENU gt MORE A gt 1 Serial Option to enter the serial option setup screen 2 Select the communication baud rate between 300 and 115 2K by pressing NEXT BAUD A until you reach the desired baud rate M Note All units in the network must be set to the SAME baud rate 7 5 HL Series Syringe Pumps Section 7 Serial Interface 7 7 User written Software 7 6 7 6 3 Verify Operation 7 7 1 DASNET 3 Select an identification number for each controller up to seven by pressing NEXT ID B until you reach the desired number M Note Multiple controllers in a network must each have a different ID number If several units are being configured place a label on the rear of each instrument listing the ID number and baud rate This will help identify the controller in the future and prevent assigning the same
21. LI SUM CSUM UNITNUM 10100 O CHR ASC 0 UNITNUM PUT UNIT ID FIRST IN OUTPUT STRING 10110 IL LEN IS GET LENGTH OF INPUT STRING 10115 REM IF INPUT IS JUST R ADD SPACE AND JMP BY CHAR 10120 IF I R THEN I I GOTO 10180 10130 O O R ADD R TO OUTPUT STRING 10140 Y HEXS IL GET OF CHAR IN INPUT STRING IN HEX 10150 IF IL 16 THEN Y 00 Y PAD OUT CHAR IN STRING IF NEED 10160 IF IL gt 16 THEN Y 0 Y IF MORE THAN 16 THEN ONLY ONE PAD 10170 O 0 Y ADD CHAR TO OUTPUT STRING 10180 O O I ADD INPUT STRING TO OUTPUT STRING 10190 IL LEN O SUM GET NEW LENGTH AND CLEAR SUM OUT 10200 FOR LI 1 TO IL TO ADD ALL ASCII FOR SUM 10210 SUM SUM ASC MIDS OS LI 1 GET THE ASCII OF LI ASCII CHAR 10220 NEXT LI 10230 REM THIS FINDS THE CHECKSUM 10235 REM THE IS FIRST SUBTRACTED FROM 256 10236 REM THEN ANDED WITH 255 TO AND OFF EXTRA BITS 10240 CSUM 256 SUM AND 255 GET CHECK SUM 10245 IF CSUM lt 16 THEN O O 0 PAD OUT CSUM IF NEED 10250 O O HEX CSUM PUT AT END OF OUTPUT STRING 10270 RETURN DONE RETURN 20000 REM THIS SUBROUTINE SENDS O TO THE COM PORT 20010 PRINT 2 CR SEND CR TO COM PORT 20020 PRINT 2 O SEND O TO COM PORT 20030 PRINT 2 CR SEND CR TO COM PORT 20040 RETURN DONE Note Polling is part of the DASNET definition but is not required and is not shown in this example If this progra
22. Press Hold and then Recall to access the new program This feature is used mainly in applications where it is necessary to keep the system pressurized during method changes External Start When a gradient program is in Hold mode a momentary low on digital input 2 of the controller ACCESSORY connector will start the program When a gradient program reaches the end there are four selectable actions the system can then perform Hold the final value example below e Stop after the final step Return to the initial value and hold it Return to the initial value and repeat the program While the system is in gradient mode these options can be edited at any point before or after gradient programming and while a gradient is running To access the options menu from the home screen press OPTIONS D GRADIENT ACTION HOLD FINAL VALUE NEXT ACTION PREVIOUS To scroll through the four options press NEXT ACTION A When you have reached the desired option press PREVIOUS D to save and exit HL Series Syringe Pumps Section 5 Continuous Flow Introduction Installation amp Operation 5 1 Introduction 5 2 Continuous Flow Air Valves Air Valve Package Numbers A HL Series continuous flow pumping system consists of two syringe pumps and a valve accessory package regulated by one controller This system allows you to continuously deliver liq uefied gas or liquid under constant flow or constant pressure m
23. and Limits lese 3 1 3 2 2 Programming Screens 0 cece eee res 3 1 3 2 3 RUM Screens u 2m A ente ee ee PARLE gH 3 2 3 2 4 Selecting Operating Parameters 0 0 cece eee eee eee 3 3 3 3 Main Menus os psa ske ihe be oe WR EEA Lees See EES YR Ge er RI OE Raw pi 3 3 3 4 Men One wa sans ae ae AU ae RE UR UT 3 3 3 4 1 Flow and or Pressure Units of Measure 0 00 eee e eee eens 3 3 3 4 2 Pump Selection Gf multiple pumps x orakel a ES 3 4 A NE ae 3 4 3 4 4 Power Failure STOP 0 0 ccc cee n 3 5 3 4 5 System Reset Restore Default Settings oooooooooooooo 3 5 3 4 6 Display Contrast oooooooorrrrr eee rn 3 5 3 5 Menn Two s r es Gtr area CRETA LS ERI 3 5 xi HL Series Syringe Pumps Table of Contents xii 3 5 1 Serial Option 2 nese ea eek Lui ne nn AT ET 3 6 3 0 2 Pump Status dil era Qh odo eA ORA A EAE UE EN E ERR E 3 6 3 5 3 External Control 2 2 2 1 2 la a Uu 3 6 3 5 4 Multiple Pumps ooooooorr eh ns 3 6 3 5 5 Total Volume Reset tinei a a E A E een tent n nes 3 6 300 Ll S MEL 3 7 3 6 Menu Three ansehen 3 7 3 6 1 Poor Fill Alarm ii E EIE ars 3 7 3 6 2 Diagnostic Menu esee 3 7 3 6 3 Pressure Calibration 0 00 cette eens 3 7 3 6 4 Diff Modes teni ta ee es AA Raed Shae es 3 8 3 6 5 Press Integral n meree Sea cai fatale elu lap ser EEG 3 8 3 6 6 Pressure Deadband 0 0c ccc ee eee
24. 0 0 ieoi aenga e e eee een tent n nee 1 14 Section 2 Fluid System Connections amp Accessories 2 1 Introduction es se eee ew deve he ROWSE eee DH EEE ee 2 1 2 2 Fluid System Connections susuan cece ene 2 1 2 2 1 Ports RIA SE He Gi E oe ee Pave bee dda eee es 2 1 2 3 Fluids Certified for Isco HLs Pumps 0 0 eee eee eee 2 2 2 3 1 Installation Tips aca ak pe REN EV S 2 2 2 3 2 Tubing Requirements 0 cece cette tenes 2 2 2 3 3 Accessory Packages oii sucat iein pe ende Deedee Nr page ae des p 2 3 2 3 4 Draining Overflow 0 cece hh me 2 3 2 3 5 Cylinder Washing Wash Gland 0 cece eee eens 2 4 2 3 6 Cylinder Washing Nitrogen Purge 0 00 ccc cence eens 2 5 2 4 Fluid Connection Accessories llle 2 6 2 4 1 Manual Refill Kit common lI Re eg d e 2 6 2 4 2 Manual Outlet Valve Kit 0 0 0 0 ccc rrr 2 7 2 4 8 In Line Filter Package 0 0 ccc cette eens 2 9 2 5 Temperature and Pressure Controls 00 0 c eee ene 2 10 2 5 1 Cylinder Insulating Cover 20 0 ccc ro 2 10 2 5 2 Temperature Control Jacket llle lees 2 11 2 5 3 Fluid Temperature and Volume 000 cee cece eens 2 12 2 5 4 Back Pressure Regulation 0 0 cece cece ees 2 13 Section 3 Basic Programming and Operation IL Introdu ction voee ed NS RE e Ener CR ES 3 1 3 2 General Controller Information 00 0 3 1 3 2 1 Rates Units
25. 40 50 60 70 80 90 100 110 120 130 140 150 Refill Flow Rate ml min Pressure bar Refill Flow Rate ml min Maximum Pressure bar 0 001 to 250 137 9 250 to 408 566 Refill Flow Rate x 0 4364 Figure 1 4 1 000HL refill flow rate range 1 7 HL Series Syringe Pumps Section 1 Introduction 1 3 Unpacking 1 4 Controls and Indicators After removing the pump controller and accessories from the shipping carton examine them for signs of shipping damage Be sure no internal parts have shaken loose in transit If there is any shipping damage file a claim with the delivering carrier immediately Compare the contents of the boxes with the enclosed packing slip If there are shortages contact Teledyne Isco immediately The pump controller regulates all pumping functions It is designed to sit on top of the pump base but may be located else where according to safety and convenience Programming and setup are performed using the keypad on the front panel The controller front panel is shown in Figure 1 5 and described in Table 1 5 Table 1 6 explains the key functions The rear panel of the pump controller contains several input and output connectors explained in Table 1 7 and shown in Figure 1 6 The only operational control on the pump itself is the power switch shown in Figure 1 8 The rear panel has several con nectors describ
26. Changeover 00 ccc cece eee nen eens 8 10 8 7 2 Liquid Solvent Changeover and Flushing 0 0 00 000 us 8 10 List of Figures 1 1 100HLf refill flow rate range 0 cette eens 1 6 1 2 260HL refill flow rate range 0 eee eee een eens 1 6 1 3 500HL refill flow rate range 0 cece ee ees 1 7 1 4 1000HL refill flow rate range 2 0 eee ee ne 1 7 1 5 Pump controller key functions 0 ccc ce eee eens 1 8 1 6 Pump controller rear panel connectors aavuvrvr ravner narre ner 1 10 1 7 Pump rear panel connectors aranranrnnrenrnvrnarnarrv raner 1 11 1 8 Pump front panel controls 0 0 00 cece eee eens 1 12 19 Status Sereen vu see ko GRE SEE TATT MANE SETT SANE 1 14 1 10 Four pump stop menu ssseeeee e 3 un 1 15 1 11 Three pump stop menu lesse rn 1 16 2 1 Drain tube installation 0 0 cece een eens 2 4 2 2 Drain tube installation and wash gland connection 00000005 2 5 2 3 Purge connector installation 0 0c ett eens 2 6 2 4 Refill kit installation 0 0 cece m hme 2 7 2 5 Outlet valve package connection 0 0c cece ete eens 2 9 2 6 In line filter package 0 ccc raneren rar nen eens 2 10 2 7 Cylinder insulation cover 0 ccc cee eect eens 2 11 2 8 System with temperature control jacket installed 2 12 2 9 Back pressure regulator 0 ccc
27. Controller Rear Panel Connectors 00 cee eee eee eee 1 10 Pump Rear Panel Connectors 0 cece cee eee eens 1 12 Pump Front Panel a ie nee en 1 13 HL Series Syringe Pump Accessory Packages 0 0 0 ce eee eee ee ees 2 3 Manual Refill Kits owe ee o e TE Sater enke ee A 2 7 Manual Outlet Valve Kits esee hh 2 9 Optional in line Filter Package 68 1247 011 ooooooooooooomo oo 2 9 Recommended Circulating Bath Volumes ooooooooooonoooooo 2 13 Key functions in the Multi pump Mode 000 cee eee eee 3 13 Manual Refill Valve Kits sese hen 4 1 Manual Outlet Valve Kits sese hh 4 2 Swaging Detail srie nen eU SG 4 2 Continuous Flow Technical Specifications Air Valves oooooo 5 2 Accessory Control Digital Outputs vavaravrav ravner rev eens 5 6 Key functions in the Multi pump Mode 0 0c c cece eee renn 5 8 Modbus TCP IP Configuration Options 0 0 cece eee eee 6 3 Modbus RTU Configuration Options 00 cece eee eee 6 3 Supported Modbus Function Codes 0 e cece een eee 6 3 Exception Responses 0 eee eee hr hae 6 3 COIS eere SEAS wad Bre RE RIO PS AD TET eee MA 6 4 Holding Registers 0 cece ete e eee e eens 6 8 RS 232 External control connector serial pin connections ceser rese daeu e a e i a eee nent s 7 2 USB Interface Pin Connections on the USB Ethernet conne
28. DRIFT 0 25 of full scale within 48 hours at constant temperature PRESSURE DISPLAY RESOLUTION AMBIENT TEMPERATURE RANGE TEMPERATURE DRIFT 6 895 kPa 5 to 40 C x 0 15 of full scale C HUMIDITY 9596 maximum CYLINDER CAPACITY 507 38 ml DEAD HEADSPACE VOLUME 4 00 x 0 020 ml POLLUTION DEGREE INSTALLATION CATEGORY MAXIMUM ALTITUDE Using water at 137 9 bar and a temperature controlled environment at 30 C b The analog output is an optional accessory c Pressure repeatability specification is based upon re zeroing pressure transducer every 48 hours Refer to sub section ZERO PRESS in Section 3 of the manual for re zeroing procedure d Volume in and above the piston seal head clearance at automatic shutoff and inlet and outlet ports to the fittings 1 4 HL Series Syringe Pumps Section 1 Introduction Table 1 4 1000HL Technical Specifications POWER REQUIREMENTS 117 12 Vac 3 2 A maximum 234 23 Vac 1 6 A maximum Jractory Set A see section 1 5 Installation Instructions LINE FREQUENCY 50 or 60 Hz LINE VOLTAGE NOISE TOLERANCE 1 7 x nominal rms line voltage 10 second pulses any phase angle random or repetitive DIMENSIONS PUMP CONTROLLER Width 27 18 cm 27 18 cm Depth 46 74 cm 30 48 cm Height 102 36 cm 13 59 cm PUMP CONTROLLER 38 5 kg 2 96 kg FLOW RATE RANGE 1 0 ul min to 408 ml min at any pressure from 0 to 137 9 bar FLOW RATE ACCURACY 0 5 maximum
29. Outlet open or closed Inlet open or closed Outlet open or closed Inlet open or closed Outlet open or closed Inlet open or closed Outlet open or closed Alternate connections can be made to drive low power 12 15 V relays or electric valves Assume two wires per relay or valve The first wire is connected to the appropriate digital output on the back of the pump controller The second wire can be con HL Series Syringe Pumps Section 5 Continuous Flow Introduction Installation amp Operation nected to the 15 V connector to supply a maximum of 200 mA for switching of relays or valves Observe correct polarity if the valve or relay is polarized 5 7 HL Series Syringe Pumps Section 5 Continuous Flow Introduction Installation amp Operation 5 4 Continuous Flow 5 8 Mode 5 4 1 Defining Operation Once your valve package has been properly installed and you have ensured that fluid connections are leak free the system is ready for operation Teledyne Isco convention is to name the pumps pump A and pump B reading from left to right Before running in continuous flow mode become familiar with independent mode which allows the controller to operate two pumps independently and simultaneously You must operate the two pumps manually for initial setup i e refill and purging of air If the Teledyne Isco air valve package is used the air valves are switched thro
30. Press and hold the CLEAR ENTRY key on the front panel keypad 3 While holding the CLEAR ENTRY key apply power to the pump controller Keep the CLEAR ENTRY key pressed for one second 4 Release the key 5 Execute a system reset described next A system reset completely clears any user programmed settings It will erase all programs and return units and limits to factory settings If the ZERO PRESS key has been used the corrected offsets will be lost This resets all pumps that are connected to the controller 1 Press the orange MENU key 2 Select number 5 SYSTEM RESET 3 Press softkey A CONTINUE M Note Preliminary checkout the pump is performed without fluid in the pump 1 The display will briefly show the software revision on the first line and the pump model s connected to the control ler on the following lines Figure 1 9 PUMP CONTROLLER ISCO INC REV lt PUMP TYPE gt 2 Check the upper left corner of the controller screen The current pump mode will be presented in a two letter abbre viation e g CF for constant flow This will be followed by a lowercase letter indicating the current pump e g lower case a indicates that pump A is the current pump The current pump is the one for which parameters are being set a If a pump other than pump A is currently selected On the lower right corner of the screen directly over softkey D are the words SELECT PUMP DAS Di HL Series
31. S xx LIMITS LIMITSB LIMITSC LIMITSD Gradient Step Upload command This transfers a step from the PC to the controller Refer to 7 8 3 for complete information on Gradient step download commands The pump needs to be in local mode only Returns the pressure and flow rate limits LOCAL Returns the instrument to local control Front panel control is enabled and all motors are stopped if control was previously remote MAXFLOWA MAXFLOWB MAXFLOWC MAXFLOWD MAXFLOWA MAXFLOWB MAXFLOWC MAXFLOWD Enter to designate the maximum flow rate setpoint Returns the maximum flow rate setpoint MAXPRESSA MAXPRESSB MAXPRESSC MAXPRESSD Enter to designate the maximum pressure setpoint MAXPRESSA MAXPRESSB MAXPRESSC MAXPRESSD Returns the maximum pressure setpoint 7 17 HL Series Syringe Pumps Section 7 Serial Interface Table 7 5 Serial Commands Continued Command Description MFLOWA MFLOWB MFLOWC MFLOWD Enter to designate the maximum flow limit in constant pressure mode MFLOWA MFLOWB MFLOWC MFLOWD Returns the maximum flow limit setpoint MINFLOWA MINFLOWB MINFLOWC MINFLOWD Enter to designate the minimum flow rate setpoint MINFLOWA MINFLOWB MINFLOWC MINFLOWD MINPRESSA MINPRESSB MINPRESSC MINPRESSD MINPRESSA MINPRESSB MINPRESSC MINPRESSD Returns the minimum flow rate setpoint Returns the minimum pressur
32. a flow rate setpoint constant flow mode Format is XXX XXXXXXX ml min Only 5 figures are significant Leading and trailing zeros are not required Gets pump information G returns a text string that contains current pressure ana log input and digital input information G amp is the Get All command This returns the same information as G plus flow rates units operation status and more For four pump operation use the G amp 2 commands Refer to 7 8 1 for a complete description of this serial command IDENTIFY INDEPENDENT INDEPENDENTCD 7 16 Pump responds SERIES 1240 0___ MODEL D PUMP REV For each pump REV is the internal pump program software revision For example if the controller was attached to two 100DMs the message would read SERIES 1240 024 MODEL 100DM PUMP SERIES 1240 024 MODEL 100DM REV__ The series number is the original catalog number for the pump type It may not match the production series number on the pump serial label SERIES 1260 008 MODEL 260HL PUMP SERIES 1260 009 MODEL 500HL PUMP SERIES 1260 007 MODEL 100HLX PUMP SERIES 1260 010 MODEL 1000HL PUMP SERIES 1260 015 MODEL 260HLf PUMP SERIES 1260 011 MODEL 500HLf PUMP SERIES 1260 014 MODEL 100HLf PUMP SERIES 1260 016 MODEL 1000HLf PUMP Put pumps in Independent mode HL Series Syringe Pumps Section 7 Serial Interface Table 7 5 Serial Commands Continued Command Description IPUMPA 1 IPUMPA 0 Tu
33. converts your direct serial commands into a form rec ognizable to our instruments Using a computer language such as C or BASIC serial commands can be entered converted and then sent to your instrument 7 7 2 Universal Driver from Teledyne Isco HL Series Syringe Pumps Section 7 Serial Interface Table 7 3 is an example BASIC language program which per forms the required portion of the DASNET serial protocol Table 7 4 is an example of a C language program which does the same Both of these programs including a DASNET serial driver written in Visual C are available on CD ROM P N 60 1245 096 in the sleeve at the back of the printed manual The Universal Driver from Teledyne Isco is a serial software driver that can translate commands for a variety of compilers When you are creating programs for HL Series syringe pumps the driver converts serial commands to DASNET communica tions protocol sends them to the serial port and waits for a serial response M Note On our website Detailed information about the Universal Driver is provided in Isco Technical Bulletin TB19 Universal Driver Software Tool LabView operation is discussed in detail in Isco Technical Bul letin TBO6 Basic Operation of LabView Toolkit A copy of the driver can be found on the CD included in the back of your HL Series pump user manual It can also be downloaded from http www isco com products appnotes asp PL 105 Open the Software Utilities fol
34. input 2 of the accessory connector See NOTE 1 Status of the analog voltage on analog input 1 of the accessory connector See NOTE 1 Status of the analog voltage input for Press D See NOTE 1 Status of the analog voltage on analog input 4 of the accessory connector See NOTE 1 CONTIN CONST FLOW CONTIN CONST FLOWCD Stops all motors sets flow rate and pressure setpoints to zero Puts pump in continuous flow under constant flow mode CONTIN CONST PRESS CONTIN CONST PRESSCD Puts pump in continuous flow under constant pressure mode CONTIN MODIFIER Put pump in continuous modifier addition mode CONST FLOW CONST FLOWB CONST FLOWC CONST FLOWD Put pump in constant flow mode CONST PRESS CONST PRESSB CONST PRESSC CONST PRESSD Put pump in constant pressure mode DELIVER DELIVERCD Set the dual pump mode to deliver fluid when running DIGITAL Returns the status High or Low of the digital outputs Format is digital xxxxxxxx where x is either H or L The status order returned corresponds with the outputs 1 8 X no change DIGITAL xxxxxxxx 1 8 TR Sets the digital output either High or Low where x is either H or L The order cor responds with the outputs 1 8 7 15 HL Series Syringe Pumps Section 7 Serial Interface Table 7 5 Serial Commands Continued Command Description DIG CONTROL Returns the
35. instrumentet eller operat ren ar det n tv ndigt att anv ndaren har fullst ndiga kunskaper i det engelska spr ket och dess tekniska termer eller utbildas av en person som tidigare brukat instrumentet gt e e e eee For at undg skade p produktet eller p brugeren er det n dvendigt at brugeren til fulde forst r det engelske sprog for at forst den tekniske formulering i den engelske manual I modsat fald skal brugeren modtage tr ning inden apparatet tages I drift HL Series Syringe Pumps Warnings Cautions and Notices Laitteelle tai k ytt j lle aiheutuvien vahinkojen v ltt miseksi on t rke ett k ytt j hallitsee englannin kielen ja englantilaiset tekniset termit tai on saanut k ytt opastuksen englantia osaavalta henkil lt Per evitare danni allo strum nto od incidenti all operatore necessario che l utilizzatore abbia una completa conoscensza della lingua inglese oppure che venga istruita da una persona che abbia utilizzato precedentemente questo strumento Para impedir qualquer dano no aparelho ou ferimentos parao operador necessario que o utilizador tenha um conhecimento completo da lingua inglesa e dos respectivos termos t cnicos ou seja treinado por uma pessoa que tenha esse conhecimento antes de operar com este aparelho Tia tnv anoduyn DAa mno tov opyavov N TPAULHATLOLOL Tov XPNOTN ELVAL ATAPALTNTO o YPNGOTNG va yv piCEl KAAG tny AYYALKA Y NGGU
36. its steps one at a time as discussed in the next section 4 5 Review Revise amp Hold Options When the last remaining step is deleted the entire file is removed HL Series Syringe Pumps Section 4 Gradient Pumping for Pressure Flow and Concentration Modes 4 5 Review Revise amp Hold Options 4 6 Program Conclusion 4 10 While in the programming menu you can also Delete To delete the current step press DELETE D A deleted step cannot be recovered Used repeatedly this command can be used to delete an entire file Review To review existing program steps press STEP BACK A or STEP FWD B Add New To add a new step between two existing steps nav igate through the program to the step just before your addition Press INSERT C and program the new step Note that the initial value of the next step will default to the final value of the new step and may need to be edited if a dif ferent initial value is needed While Running A gradient program can be reviewed or edited while it is running Simply press EDIT B or REVIEW C to begin If a new step duration is shorter than the elapsed time for that step the program will proceed to the next step If the total flow rate is changed the program will immediately start using the new rate To return to the run screen press RETURN D Hold You can hold a running gradient in its current state while retrieving a different program file to run in its place
37. least six inches longer in each basic dimension than the unit Fill the box equally around the unit with resilient packing material e Seal it with strapping tape and ship it to the address on the warranty The warranty at the end of the manual HL Series Syringe Pumps Section 8 Pump Maintenance Troubleshooting and Servicing also describes the conditions under which Teledyne Isco will pay surface shipping cost NOTICE Do not return the pump without contacting the Teledyne Isco Technical Service Do not return the pump without first providing written guarantee that it has been decontaminated of hazardous or potentially lethal materials Teledyne Isco reserves the right to refuse shipment if no decontamination assurance has been provided prior to shipment Failure to decontaminate a pump may result in legal action taken by state or federal authorities 8 2 General Cleaning 8 3 Resetting the System 8 3 1 Basic Reset 8 3 2 Hard Reset M Note It is very important that the shipment be well packed and fully insured Damage claims must be settled between you and the carrier This can delay repair and return of the unit to you For general cleaning of the instrument s front panel or enclosure use a mild detergent in water or isopropyl alcohol on a sponge which is mostly squeezed out This action completely deletes user programmed settings erasing all programs and returning the controller to factory default sett
38. may damage critical components resulting in property damage personal injury or even death Users who pump fluids other than those listed below do so at their own risk The fluid contacting materials within the HL Series Syringe Pump have been tested and certified to be compatible with the following fluids ethane ethylene propane propylene butane butadiene hexane gasoline diesel fuel e jet fuel ammonia methanol e isopropanol acetone acetonitrile heptane benzene e ethylbenzene methane Consult with Isco before using any chemical not listed above HL Series Syringe Pumps Warnings Cautions and Notices WARNING PLEASE READ At the request of our Supereritical Fluid Extraction laboratory staff we want our customers to be aware of the potential hazards involved with supereritical fluid extraction Oxidizing gases such as nitrous oxide in contact with organic matrices or flammable modifiers can detonate under certain conditions Likewise flammable fluids such as methane under high pressure conditions can present a hazard With concern for the safety of our customers we have designed our extractors to be as safe as possible However we do not recommend the use of our instrument with potentially explosive reactions The letter below which appeared in the July 22 1991 edition of Chemical and Engineering News is reprinted with permission from Professor Robert E Sievers and his colleagues at
39. non hazardous Table 1 7 Pump Controller Rear Panel Connectors Item No on Connector Description Figure 1 6 PUMP D This connector is only used during multiple pump operation The control cable from the rear panel of the fourth pump is attached to this connector PUMP C This connector is only used during multiple pump operation The control cable from the rear panel of the third pump is attached to this connector PUMP B This connector is only used during multiple pump operation The control cable from the rear panel of the second pump is attached to this connector PUMP A This plug connects the control cable from the pump rear panel This connec tion should be secured with the thumbscrews IMPORTANT The pump A connector is the only input power connector on the rear panel of the controller During single pump operation the pump must be attached to this connector to supply power to the controller AN WARNING Do not connect or disconnect the control cable when the pump is con nected to the mains voltage HL Series Syringe Pumps Section 1 Introduction ACCESSORY These terminals allow connection of input and output signals such as analog controls and external RUN STOP RS 232 RS 485 This serial port connector may be used with an RS 232 or RS 485 cable to place the pump under remote control Refer to Section 7 for RS 232 and Section 6 for RS 485 pin connections USB ETHERNET This connector may be used
40. number for your unit Then go to our Web site at www isco com In the left column under Service amp Support click Schematics Enter your serial number in the field provided and click Get Schematics If you need assistance or don t see the correct sche matic for your specific unit contact our service department If you have a question about a procedure need parts information or need some help call or email the Teledyne Isco Service department In your message be sure to include all the details about your instrument and the nature of the error Call the Service Department before returning the unit for factory repair Often a problem can be solved in the field with just a little extra help Contact us at Toll free 800 775 2965 Outside USA Canada and Mexico 402 464 0231 Email IscoService teledyne com In the rare event that an instrument must be returned for main tenance the following measures must be taken to ensure a proper return Teledyne Isco Technical Service 800 775 2965 must be contacted prior to shipment to obtain a clean return form The applicable MSDS paperwork of the last substance ran must be received by Technical Service The syringe pump must be shipped with the cylinders removed from the pumps and any residue completely rinsed with methanol or water Wrap the unit in heavy paper or a plastic bag If the original box is not available put the wrapped unit in a strong cardboard box at
41. rates are 1200 and 19200 Other baud rates of 300 1200 2400 4800 9600 19200 38400 57600 and 115200 are supported by the HL Series pumps but are not part of the Teledyne Isco defined communica tions standard Electrical standards are RS 232 C connector pin usage is out lined in Table 7 1 Characters consist of 1 start bit 8 data bits low order first with 8 bit always set to zero and 1 stop bit There is no parity bit used All characters will be printable ASCII characters Control characters 0 1FH are ignored except for car riage return 0DH The serial unit number and baud rate can be changed from the default values through the MENU key Select SERIAL under the menu and adjust the values using the softkeys AN WARNING Connecting devices to energized circuits may cause personal injury or property damage Power must be removed from the pump before connecting external devices Table 7 1 RS 232 External control connector serial pin connections Name Use CHASSIS GROUND Used to connect to the shield of the interconnect cable RECEIVE Serial interface data input Standard RS 232 C signal levels TRANSMIT Serial interface data output Standard RS 232 C signal levels REQUEST TO SEND RTS chain RS 232 C input is buff ered and connected to pin 21 CLEAR TO SEND CTS buffered RS 232 C output of pin 25 input 11 VDC DATA SET READY is held on COMMON Signal common for all signals
42. run screen use softkeys MORE and D PRE VIOUS RETURN Use the number keys to select a menu option Selecting a menu option displays the programming parameters for that option in menu form To exit a menu when multiple pumps are connected press the STORE RECALL CLEAR ENTRY OR MENU key Menu 1 provides programming options for units of measure pump selection refill settings power failure response system reset and display contrast adjustment To save all settings and return to the main menu press RETURN D UNITS 4 POWER FAILURE STOP SELECT PUMP 5 SYSTEM RESET REFILL 6 DISPLAY CONTRAST MORE RETURN Figure 3 1 Menu 1 program selections 3 4 1 Flow and or Pressure Units of Measure Figure 3 2 Units menu Displayed units are user selectable by selecting menu 1 item 1 PRESSURE UNITS FLOW UNITS 1 ATM 3 PSI 5 mL MIN 7 uL MIN 2 BAR 4 kPA 6 mL HR 8 uL HR PREVIOUS A B C D 3 3 HL Series Syringe Pumps Section 3 Basic Programming and Operation 3 4 3 4 2 Pump Selection if multiple pumps 3 4 3 Refill To set auto refill volume To set refill rate Disable Enable auto refill Use numbers 1 4 to select the pressure units ATM BAR PSI kPa The units selected will be displayed on the first line after PRESSURE UNITS Use numbers 5 8 mL MIN mL HR uL MIN uL HR to set the flow rate units The selected units will be displayed on the first line after FLOW UNI
43. the University of Colorado at Boulder Even though they were not performing supercritical fluid extraction it details the problems their lab experienced using nitrous oxide under simi lar conditions We add our support for their suggestion to use only carbon dioxide or other less hazardous fluids for supercritical fluid extraction ERING CHEMICAL amp ENGINE LETTERS G per 29 69 Num 1991 69 29 jus JULY 22 234 N 0009 E 1 F jon ethano EMICA LSA oxide Ne en ethanol super fluid f s 500 mL mp for omatob ica the g jon tally at po ed ex id extract A ten any others h with p i mix gon E e Or ap xide thanol is py a MO traction OF IG ers 9 A flui p e detonated i po uid hro os superent hy x catalyst p supert anol gt chromatog P ite nazar aratus Mnough pade as UT n this Ca E plosion p pue c jence n the xp samp Wee p pe retraeth gdizer le ous oxide yhen EA ethane triethy po vr nat can POT 4 t 7 Thi u tu oxide WI ate O sp th P i T rthosilic th 1pho 2100 psi adr red scientis N 9 07 viet sed ue with D ve dempere OF Sa we ixture W Pei less st 1 A Because Res this g other less o m ex at 40 pr imate npn usty may ju carb dior gupstitut vers an othe mix ur pu Roo dous sol Robert fc gt omistry the t Jes essor 0 nsen pos the re equi pmen rete a per pe ant pt s surround 9 in a CO dam Research id into ng one fitting it deal of alot Colorado bed Y doing a h univers
44. the pressure setpoint SETPRESSB SETPRESSC SETPRESSD STATUSA Returns with status of pump May be any combination of responses listed below STATUSB STATUSC STATUSD STATUS STOP PROBLEM OVER PRESSURE RUN UNDER PRESSURE REFILL CYLINDER FULL HOLD CYLINDER EMPTY EQUIL MOTOR FAILURE LOCAL REMOTE EXTERNAL Same as front panel except that pump remains under remote serial control UNITSA Enter the desired flow or pressure units after the equal sign Acceptable values are ATM BAR KPA PSI ML MIN ML HR UL MIN UL HR Sets all pumps Return the volume remaining in cylinder in ml Format is XXX XXXX ml VOLTOT Returns the total volume delivered when using continuous flow or modifier VOLTOTCD VOL RESET Will reset the volume total to zero VOL RESETCD Zeros the pressure sensor offset ZERODIFF1 Zeros the pressure sensor offset for the respective analog input ZERODIFF2 ZERODIFF3 NOTE 1 The analog input range is 1 5 to 11 6 volts There is NO conversion of the returned number The number returned 0 to 65535 decimal will have an offset of 7500 added to the number 7500 0 volts and a scale of 5000 for every 1 volt for example number 7500 volts 32500 7500 5000 5000 79 Volts NOTE 2 The only pump B commands accepted in continuous pumping mode or modifier addition mode are B FLOWB LIMITSB PRESSB REFILLB REFILLB STATUSB VOLB 7 8 1 Get Status Command The G
45. toggle this feature off or on for each pump Auto refill can be set independently for each pump If AUTO REFILL is ON the pump will automatically switch to refill mode when the volume reaches the auto refill mark After refilling pumping will resume in the programmed mode The ACCESSORY outputs which drive powered valves will switch in sequence 3 4 4 Power Failure STOP 3 4 5 System Reset Restore Default Settings 3 4 6 Display Contrast 3 5 Menu Two HL Series Syringe Pumps Section 3 Basic Programming and Operation Unless otherwise specified the pump will refill to full cylinder capacity To specify a smaller refill volume press 2 and enter the desired volume Press 5 to toggle this feature off or on for each pump The system can also be programmed to refill a pump based on an external analog input voltage with a range of 0 to 11 5 volts Information about this feature is provided in Section 3 12 3 This feature allows you to set the activity of the pump in the event of a power failure Press 4 to toggle this feature between STOP to remain stopped after power is restored or CONT to automatically resume after power is restored M Note In a multiple pump system this action is applied to all con nected pumps To restore default program settings press 5 To continue with the reset press CONTINUE to cancel the reset press DO NOT D A CAUTION Resetting the system erases all programs and
46. with a USB or Ethernet cable Refer to Section 7 for USB pin connections and Section 6 for Ethernet pin connections 4 20mA OUTPUT Optional circuit provides 4 20mA current loop output and additional digital DIGITAL I O inputs and outputs SERIAL TAG This tag indicates the serial number of the instrument CHASSIS GROUND Ground point for high static or remote controller installations c 234 VAC men REPLACE WITH Lg TRANSDUCER tetas ES TOS Velt 17 Aspe 15 He 6060 Ton men Pe 4700 aperos Leu ME MDG 6 o FOR USE ONLY WITH MODEL HAZLOC PUMP CONTROLLER gt Ta a E o Y Pr gt wm Figure 1 7 Pump rear panel connectors Ay WARNING Explosion Hazard The area must be known to be non hazardous before servicing replacing the unit and before installing or removing I O wiring Do not disconnect equipment unless power had been disconnected and the area is known to be non hazardous HL Series Syringe Pumps Section 1 Introduction Item No on Figure 1 7 Table 1 8 Pump Rear Panel Connectors Connector Description Pressure transducer The pressure transducer cable must be plugged in for the pump to operate Control cable This cable connects the pump to the controller Chassis ground Ground point for high static installations Fuse Label 234 VAC Replace with Part Number 41 1461 264 11
47. y y and ed MYC pis have often on side such Others of nitroV umes arger V vi gt HL Series Syringe Pumps Warnings Cautions and Notices CAUTION Avoid spills Liquids associated with this instrument may be classified as carcinogenic biohazardous flammable or radioactive Should these liquids be used it is highly recommended that this application be accomplished in an isolated environment designed for these types of materials in accordance with federal state and local regulatory laws and in compliance with your organization s chemical hygiene plan in the event of a spill In all cases when using Teledyne Isco instrumentation prudence and common sense must be used WARNING Pinch point This symbol warns you that your fingers or hands will sustain serious injury if you place them between the moving parts of the mechanism near this symbol WARNING Avoid hazardous practices If you use this instrument in any way not specified in this manual the protection provided by the instrument may be impaired this will increase your risk of injury gt PE CAUTION Liquids associated with this instrument may be classified as carcinogenic biohazardous flammable or radioactive Should these liquids be used it is highly recommended that this application be accomplished in an isolated environment designed for these types of materials in accordance with federal state and local regulatory laws and in complia
48. 1 5 ul min seal leakage FLOW RATE DISPLAY RESOLUTION 1 0 ul min ANALOG OUTPUT ACCURACY 1 of selected range DISPLACEMENT RESOLUTION 25 36 nl REFILL TIME 2 5 minutes REFILL OR DEPRESSURIZATION RATE 1 0 ul min to 408 ml min for any pressure up to 68 9 bar For higher pressures see Figure 1 4 PRESSURE RANGE 0 6895 to 137 9 bar PRESSURE ACCURACY 0 5 of full scale at constant temperature PRESSURE REPEATABILITY 0 5 of full scale within 48 hours at constant temperature ZERO PRESSURE DRIFT 0 25 of full scale within 48 hours at constant temperature PRESSURE DISPLAY RESOLUTION 6 895 kPa AMBIENT TEMPERATURE RANGE 5 to 40 C TEMPERATURE DRIFT 0 12 of full scale C HUMIDITY 95 maximum CYLINDER CAPACITY 1015 0 ml DEAD HEADSPACE VOLUME 11 0 0 7 ml POLLUTION DEGREE INSTALLATION CATEGORY MAXIMUM ALTITUDE A team lift is recommended when moving this instrument Using water at 137 9 bar and a temperature controlled environment at 30 C The analog output is an optional accessory Pressure repeatability specification is based upon re zeroing pressure transducer every 48 hours Refer to sub section ZERO PRESS in Section 3 of the manual for re zeroing procedure e Volume in and above the piston seal head clearance at automatic shutoff and inlet and outlet ports to the fittings ao Tp 1 5 HL Series Syringe Pumps Section 1 Introduction 100HL Refi
49. 1 STORE TO EXIT 1 INIT 3 RATE 0 00 MIN 2 FIN 4 DURATION 1 0MIN INSERT DELETE 6 To set the initial pressure or flow for this step press INIT 1 to activate this parameter Use the number keys to enter the desired value then press ENTER to save it 7 To set the final pressure or flow for this step press FINAL 2 to activate this parameter User the number keys to enter the desired value then press ENTER to save it 8 Set either the desired RATE 3 of change or DURATION 4 in minutes Once one value has been set and saved the other will automatically appear M Note DURATION in minutes can have a resolution of 0 1 with a maximum of 9 999 minutes per step 9 If you want to add another step to the file program press INSERT C 10 The step number will increase by one and the default ini tial value will be the final value entered for the previous step Edit as desired 11 When programming is complete press the STORE key to save the file and return to the home screen 12 To start the program press RUN two times M Note When a gradient run is started digital output 8 of the controller ACCESSORY connector will toggle from high to low open to closed for one second The flow rates and ramp rate for Pump in each step will be in direct opposite proportion to the values set for Pump B INIT B FIN B and RATE Note that an entire gradient program can be removed only by deleting each of
50. 11 VDC DATA CARRIER DETECT is held on 7 2 7 3 USB Interface 7 4 Cabling for Serial Control 7 4 1 One Controller HL Series Syringe Pumps Section 7 Serial Interface 5 VDC Test Voltage 11 VDC Negative test voltage TRANSMIT CHAIN Serial data from next unit RECEIVE CHAIN Serial data to next unit RTS CHAIN RTS buffered RS 232 C output of pin 4 input CTS CHAIN CTS chain RS 232 C input is buffered and connected to pin 5 NOTE Only pins 2 3 amp 7 are required for serial interface to one controller The Isco HL Series syringe pump controller includes a USB interface The USB connections are located on the pump con troller rear panel labeled USB Ethernet The connector pin usage is outlined in Table 7 2 The drivers for the USB can be down loaded from http www isco com support updates asp Ay WARNING Connecting devices to energized circuits may cause personal injury or property damage Power must be removed from the pump before connecting external devices Table 7 2 USB Interface Pin Connections on the USB Ethernet connector Pin Number Name 7 GND 11 Data 12 Data The cabling scheme for your system will depend on the number of instruments you need to control The computer is always con nected from the serial port to the serial port s of the HL Series controller s it is controlling Each controller is connected to its pumps in the norm
51. 2 with a pressure range of 5000 psi at 5 volts PRESSCNTRLNORM Sets the pressure control input to the standard input PRESSDIFF XXXXX Differential pressure setpoint PSI 10 0 to 50 000 maximum 0 to 5000 psi PRESSDIFF Reads the differential pressure value PSI 10 The transducer can also be read via the ALOGx serial commands RANGEA RANGEB RANGEC RANGED RAPIDA RAPIDB RAPIDC RAPIDD Provides scaling information for the system parameters See 7 8 4 for more informa tion about this serial command Activates the automatic rapid pressurization cycle constant flow mode only RECEIVE RECEIVECD Set the dual pump mode to receive fluid when running REFILL REFILLB REFILLC REFILLD REFILL REFILLB REFILLC REFILLD REMOTE Move cylinder to bottom at preset refill rate Enter to designate refill rate Disables controller front panel control and enables all serial commands Stops all motors if control was previously local RLIMITA RLIMITB RLIMITC RLIMITD Returns the refill flow rate limit RSVP RSVPB RSVPC RSVPD RUN RUNB RUNC RUND RUNALL Pump responds with READY message Same as front panel Initiates pumping SETFLOWA SETFLOWB SETFLOWC SETFLOWD Returns the flow rate setpoint 7 19 HL Series Syringe Pumps Section 7 Serial Interface Table 7 5 Serial Commands Continued Command Description SETPRESSA Returns
52. 7 VAC Replace with Part Number 41 1461 271 Main Fuses Call Teledyne Isco for replacement AC Main Power 120 Volt Systems Green Yellow Earth Black AC hot White AC neutral 234 Volt Systems Green Yellow Earth Brown AC hot Blue AC neutral IN WARNING Do not separate connections when circuits are energized AC main power must be permanently wired to the AC power source Never attach a plug or power cord to the wires All input and output circuits must be wired using Division 2 wiring methods as specified in Article 501 4 b of the National Electrical Code NFPA 70 for installations in the U S In other countries local codes apply JL PULL ON PRESS OFF Item No on Connector Figure 1 8 HL Series Syringe Pumps Section 1 Introduction Table 1 9 Pump Front Panel Description 1 Mains power switch 1 5 Electrical Connections Disconnects power from the pump circuits for setup changes such as connecting the controller 1 mains power is applied to the pump circuitry 0 mains power is removed from the pump circuitry The pump controller may be placed on top of the pump Power is supplied to the pump controller through the control cable AN CAUTION All connections between the pump and controller should be made BEFORE the pump is connected to mains power 1 Connect the pressure transducer cable which originates from the top of the pump cylinder to the nine pin
53. 70 C While the High Temperature pump seals are rated up to 200 C due to the jacket seal rating do not exceed 100 C For pumps using virgin PTFE seals contact the factory for assistance HL Series Syringe Pumps Section 2 Fluid System Connections amp Accessories Volume Fluid bath volume is determined by cylinder volume To maximize temperature stability a minimum of twice the cylinder volume should be used Refer to Table 2 5 for recommended fluid volumes Table 2 5 Recommended Circulating Bath Volumes Pump Model Min Volume ml 100HL 205 86 260HL 532 10 500HL 1014 76 1000HL 2030 00 2 5 4 Back Pressure The back pressure regulator option from Teledyne Isco reduces Regulation flow noise and improves pump performance at pressures less than 3 5 bar Two back pressure regulators are available 7 bar part 209 9012 22 5 bar part 209 9012 21 Fittings to connect the tubing to the regulators are supplied NDANGER RISK OF INJURY THIS EQUIPMENT PRODUCES HAZARDOUS PRESSURES PLEASE UTILIZE APPROPRIATE TUBING AND CONNECTIONS AS NOTED IN THE MANUAL Installation 1 Note the arrow on the regulator indicating the fluid direction 2 Connect your tubing between the pump outlet and the reg ulator using the supplied fittings 2 13 HL Series Syringe Pumps Section 2 Fluid System Connections amp Accessories Figure 2 9 Back pressure regulator 2 14 HL Series Syringe Pumps
54. Air Valves Pressure fluctuation at switchover during delivery Minimum 5 psi 0 35 bar at system backpressures from 100 psi 6 9 bar to the single pump maximum Higher fluctuation occurs at pressures below 100 psi 50 76 psi 3 5 bar Maximum system backpressure The single pump maximum Valves rated to 10 000 psi 689 5 bar Air supply source pressure 80 to 115 psi 5 5 to 7 9 bar Maximum flow rate ml min Liquids 65 of the single pump maximum rate Liquefied gases 1000HL 265 2 500HL 132 6 260HL 69 55 100HL 32 50 45 of the single pump maximum rate Cylinder cooling jack ets should be used to obtain this rate Temperature range 0 to 40 C Wetted materials in valve packages 5 2 1 Dual Air Valve Installation Air valves Tubing and fittings Hastelloy PEEK and PTFE SS316 gold To install the air valve package refer to Figure 5 1or 5 3 depending on your pump model 1 Position the valve bases 1 3 cm apart 2 Use the plugs to stopper the ports which will not be con nected NDANGER RISK OF INJURY THE PRESSURE PRODUCED COULD BE 700 BAR PLEASE UTILIZE APPROPRIATE TUBING AND CONNECTIONS NOTED IN THE MANUAL 3 Loosely attach the tubing lengths from the valve assembly to the pumps 4 Allow the bracket to hang vertically Install the four 4 panhead screws on bottom of the bracket and the two 6 panhead screws Plumbing connections 5 Ti
55. Alarm B ON 0 Poor Fill Alarm C OFF 1 Poor Fill Alarm C ON 0 Poor Fill Alarm D OFF 1 Poor Fill Alarm D ON 1 Stop all motor and reset flow rate and pressure set points to default 0 Transducer of Pump A not connected 1 Transducer of Pump A connected 0 Pump A not at upper flag limit 1 Pump A at upper Flag Limit Empty 0 Pump A not at lower flag limit 1 Pump A at lower flag limit Full 1 Pump A is overpressure 1 Pump A is underpressure 1 Motor failure Pump A 0 Transducer of Pump B not connected 1 Transducer of Pump B connected 0 Pump B not at upper flag limit 1 Pump B at upper Flag Limit Empty 0 Pump B not at lower flag limit 1 Pump B at lower flag limit Full 1 Pump B is overpressure 1 Pump B is underpressure 1 Motor failure Pump B 0 Transducer of Pump C not connected 1 Transducer of Pump C connected 0 Pump C not at upper flag limit 1 Pump C at upper Flag Limit Empty 0 Pump C not at lower flag limit 1 Pump C at lower flag limit Full 1 Pump C is overpressure 1 Pump C is underpressure 1 Motor failure Pump C 0 Transducer of Pump D not connected 1 Transducer of Pump D connected 0 Pump D not at upper flag limit 1 Pump D at upper Flag Limit Empty 0 Pump D not at lower flag limit 1 Pump D at lower flag limit Full 1 Pump D is overpre
56. Constant ow mode ml min ml hr ul min ul hr See NOTE 2 ml min ml hr ul min ul hr See NOTE 2 ow rate set point for multi pump pair AB in ontinuous Flow Constant Flow mode ml min ml hr ul min ul hr See NOTE 2 Continuous Flow Constant Flow mode Refill flow rate for Pump A ow rate set point for multi pump pair CD in ml min ml hr ul min ul hr See NOTE 2 ml min ml hr ul min ul hr See NOTE 2 Refill flow rate for Pump B ml min ml hr ul min ul hr See NOTE 2 Refill flow rate for Pump C ml min ml hr ul min ul hr See NOTE 2 Refill flow rate for Pump D ml min ml hr ul min ul hr See NOTE 2 Maximum pressure set point for Pump A ATM BAR kPa PSI See NOTE 1 Maximum pressure set point for Pump B ATM BAR kPa PSI See NOTE 1 Maximum pressure set point for Pump C ATM BAR kPa PSI See NOTE 1 Maximum pressure set point for Pump D ATM BAR kPa PSI See NOTE 1 Minimum pressure set point for Pump A ATM BAR kPa PSI See NOTE 1 Minimum pressure set point for Pump B Minimum pressure set point for Pump C ATM BAR kPa PSI See NOTE 1 ATM BAR kPa PSI See NOTE 1 6 8 ROP MP rf re ro rm pr ro rm rm rm rm ro Minimum pressure set point for Pump D Maximum flow rate set point for Pump A ATM BAR kPa PSI See NOTE 1 ml min ml hr ul min ul hr See NOTE 2
57. Control for Refill 2 0 0 0 00 eens 3 18 3 12 4 Calculation Examples 0 ccc cee eee eens 3 19 3 12 5 Setup sic ee c eS e s ei toes ke es 3 19 3 13 Remote RUN STOP 0 0 0 ccc eee hh 3 19 3 13 1 Wire Connections uuum het sia Gosche Gee on Gokart 3 20 Section 4 Gradient Pumping for Pressure Flow and Concentration Modes 4 1 Introduction ec REIP ORAS EES ea Ea Ss pest re Feld esse 4 1 4 2 Connecting the System 0 0 cent nen a 4 2 4 2 1 Inlet Connections 0 0 ccc eee teens 4 4 4 2 2 Outlet Connections 0 0 eee nen eens 4 5 4 3 Single Pump Gradient Programming esee eee 4 6 4 4 Dual Pump Concentration Gradient Programming 0000005 4 8 4 5 Review Revise amp Hold Options 0 0 00 4 10 4 6 Program Conclusion 0 0 0 r narr eee eee tenes 4 10 HL Series Syringe Pumps Table of Contents Section 5 Continuous Flow Introduction Installation amp Operation 5 1 Introductions 3 25 e058 Actes RR bU op UL IRAE uU LES dr 5 1 5 2 Continuous Flow Air ValvesS oooooooooo ee 5 1 5 2 1 Dual Air Valve Installation oooooooooooooooooooooo o 5 2 5 3 User Supplied Valves acoso eci ero rert idia eea Eeee aE E a 5 6 5 4 Continuous Flow Mode 0 0 ccc eee eee nee e ene 5 8 5 41 Defining Operation naci el ART E ae 5 8 5 4 2 Constant Flow Mode 22 0 ccc ccc eee tenn ee aan 5 9 5 4 8 Constant Pressure Mode
58. D to continue rapid pressurization 3 8 9 DISP The DISP key activates Dispense Mode for applications such as reactant feed and batch delivery where a specified volume is pumped Refer to Section 3 10 3 for programming steps 3 8 10 ACC CTRL The ACC CONTROL key will allow you to manually operate accessories such as valves via the Digital Output terminals on the back of the controller 1 Press ACC CTRL 2 From the accessory control menu use the number keys 1 8 1 A INLET 2 A OUTLET 3 B INLET 4 B OUTLET 5 C INLET 6 C OUTLET 7 D INLET 8 D OUTLET to toggle the desired valve open or closed Numbers 1 8 represent digital output terminals 1 8 respectively 3 To exit press PREVIOUS D 3 8 11 ZERO PRESS The ZERO PRESSURE key will correct pressure sensor drift Before pressing ZERO PRESS the pump should have port fit tings installed and be depressurized 1 Press ZERO PRESS The display will show the current pressure and ask if you want to zero the pressure 2 Press A B C or D to zero the desired pump or If the pump is not depressurized press DO NOT D to exit the zero pressure operation If four pumps are connected press the STORE RECALL OR CLEAR ENTRY key 3 12 3 9 Control of Multiple Pumps 3 9 1 Multi Pump Operation 3 9 2 Independent Control of up to Four Separate Pumps HL Series Syringe Pumps Section 3 Basic Programming and Operation When using multiple pumps there are two multi pump
59. HL Series Syringe Pumps Installation and Operation Guide lu TELEDYNE ISCO Everywhereyoulook Part 69 1243 968 of Assembly 60 1243 997 Copyright 2013 All rights reserved Teledyne Isco Rev A July 2014 HL Series Syringe Pumps Warnings Cautions and Notices Warnings and Cautions The lightning flash and arrowhead within the triangle is a warning sign alerting you to dangerous voltage inside the product The exclamation point within the triangle is a warning sign alerting you to important instructions in this manual Symboles de S curit Ce symbole signale la pr sence d un danger d lectrocution Ce symbole signale l existence d instructions importantes relatives au produit dans ce manuel HL Series Syringe Pumps Warnings Cautions and Notices Warnungen und Vorsichtshinweise Der gepfeilte Blitz im Dreieck ist ein Warnzeichen das Sie vor gef hrlichen Spannungen im Inneren des Produkts warnt Das Ausrufezeichen in Dreieck ist ein Warnzeichen das Sie darauf aufmerksam macht da wichtige Anleitungen zu diesem Handbuch geh ren Advertencias y Precauciones Esta sefial alerta sobre la presencia de alto voltaje en el interior del producto Esta se al le advierte sobre la importancia de las instrucciones del manual que acompanan a este producto HL Series Syringe Pumps Warnings Cautions and Notices To prevent damaging the instrument or injuring yourself it is absol
60. ID number to two controllers or assigning an incorrect baud rate To verify network operation open a terminal emulator program on your computer such as HyperTerminal and establish con nection with the controller M Note If you will be using multiple controllers daisy chained to a sin gle serial port you must connect and test each controller one at a time 1 Press MENU gt MORE A gt MORE A and select DIAGNOSTIC MENU 2 2 Select SERIAL TEST 6 The SENT value displayed will increment indefinitely until you exit the test by pressing any key This same value should appear on your computer screen If it does not verify that you are using the correct serial port on the computer and that the communi cation cables are properly connected and in working order When designing software to control the HL Series pumps you must follow the DASNET communications protocol This protocol allows a number of instruments to be controlled from a single RS 232 C serial port Up to seven HL Series controllers may share a single serial data channel with each controller only accepting commands that are meant for it Each pump controller can then control up to three pumps each Figure 7 1 shows a simple system where the PC is connected to the serial port on the rear panel of the pump controller shown sitting on top of the pump module The pump control cable is attached to the pump A connector on the rear panel of the controller DASNET
61. If you attempt to run the program without changing the mode of either it or the controller the program will not run and the same message will be displayed 4 To enter the flow rate for this program either press FLOWRATE A and use the number keys and Enter or for maximum possible flow simply press MAX C ENTER FLOWRATE 0 00000 XX 00000 V FLOWRATE PREVIOUS MAX 5 To proceed to the programming screen press STEP FWD B The programming screen will appear with the file number and step number at the top of the screen 4 6 HL Series Syringe Pumps Section 4 Gradient Pumping for Pressure Flow and Concentration Modes Pca FILEHT STEP 1 STORE TO EXIT 1 INIT OPSI 3 RATE 0 00PSI MIN 2 FINAL OPSI 4 DURATION 1 0MIN INSERT DELETE or a b FGa FILEH 1 STEP 1 STORE TO EXIT 1 INIT 3 RATE 0 00 MIN 2 FIN 4 DURATION 1 0MIN INSERT DELETE 6 To set the initial pressure or flow for this step press INIT 1 to activate this parameter Use the number keys to enter the desired value then press ENTER to save it To set the final pressure or flow for this step press FINAL 2 to activate this parameter User the number keys to enter the desired value then press ENTER to save it Set either the desired RATE 3 of change or DURATION 4 in minutes Once one value has been set and saved the other will automatically appear M Note DURATION in minutes can have a resolutio
62. M Note If you make an error press CLEAR ENTRY to delete one char acter at a time 4 Press ENTER once the desired pressure is displayed 5 Press RUN to initiate pump operation For applications such as reactant feed and batch delivery where a specified volume is pumped Dispense Mode is available with Teledyne Isco syringe pumps Dispense mode flow rate begins at zero ramps up to level out at the programmed flow rate then ramps back down to zero deliv ering a precise specified volume The slope rate and run time are dependent upon the pump model being used Start Stop Specified Flow ramp up ramp down Figure 3 10 Depiction of dispense mode sequence 3 16 M Note For time controlled delivery you must run the pump in stan dard gradient mode as described in Section 4 In order to access Dispense Mode control the pump must be in Constant Flow mode Press CONST FLOW to enter this mode Toggle Dispense Mode off or on by pressing DISP Note that pressing the DISP key while in Constant Pressure mode has no effect To adjust the flow rate press FLOWRATE A enter the desired rate using the number keys and press ENTER To specify the volume of the batch delivered press VOLUME C enter the desired volume using the number keys and press ENTER 3 11 External Control 3 12 External Control Analog 3 12 1 Wire connections 3 12 2 Preparation HL Series Syringe Pumps Section 3 Basic Programming and Operati
63. PRESS STOP KEY TO STOP ALL PUMP A PUMP B PUMP C PUMP D A B C D b Otherwise you will be prompted to press A to stop pump B to stop pump B C to stop pump C or D to stop all pumps HL Series Syringe Pumps Section 1 Introduction PRESS RUN KEY TO ESCAPE PRESS ALL PUMP OR STOP KEY TO STOP ALL PUMP A PUMP B PUMP C ALL PUMP A B Cc D Figure 1 11 Three pump stop menu If you encountered any problems during the preliminary checkout please contact the Teledyne Isco Service Department The number is 800 775 2965 or 402 464 0231 HL Series Syringe Pumps Section 2 Fluid System Connections amp Accessories 2 1 Introduction 2 2 Fluid System Connections 2 2 1 Ports Standard port information This section discusses general fluid system connections and the installation of fluid connection accessories temperature and pressure control accessories and optional kits and attachments NDANGER RISK OF INJURY THIS EQUIPMENT PRODUCES HAZARDOUS PRESSURES USE APPROPRIATE TUBING AND CONNECTIONS AS INSTRUCTED M Note When operating at flow rates at or below 500 ul min it is strongly suggested that an temperature control jacket be installed See Sections All HL Series pump models connect similarly but with varying port sizes Take care to follow the tips provided to ensure safety and proper operation There are two ports in the pump standard cylinder cap Either port can serve a
64. Register Table 6 6 Holding Registers Continued of Registers HL Series Syringe Pumps Section 6 Modbus Configuration Description Maximum flow rate set point for Pump B ml min ml hr ul min ul hr See NOTE 2 Maximum flow rate set point for Pump C ml min ml hr ul min ul hr See NOTE 2 Maximum flow rate set point for Pump D ml min ml hr ul min ul hr See NOTE 2 Minimum flow rate set point for Pump A ml min ml hr ul min ul hr See NOTE 2 Minimum flow rate set point for Pump B ml min ml hr ul min ul hr See NOTE 2 Minimum flow rate set point for Pump C ml min ml hr ul min ul hr See NOTE 2 Minimum flow rate set point for Pump D ml min ml hr ul min ul hr See NOTE 2 Maximum flow limit in Constant Pressure mode for Pump A ml min ml hr ul min ul hr See NOTE 2 Maximum flow limit in Constant Pressure mode for Pump B Maximum flow limit in Constant Pressure mode for Pump C ml min ml hr ul min ul hr See NOTE 2 ml min ml hr ul min ul hr See NOTE 2 Maximum flow limit in Constant Pressure mode for Pump D ml min ml hr ul min ul hr See NOTE 2 Actual pressure of Pump A Actual flow rate of Pump A ATM BAR kPa PSI See NOTE 1 ml min ml hr ul min ul hr See NOTE 2 Volume remaining in Pump A Actual pressure of Pump B milliliters ATM BAR kPa PSI See NOTE 1 Actual flow r
65. SSE DN ER TET BARA IE N gt X Represent the concentration of the hazardous substance in this component s at least one homogeneous piece is higher than the ST standard limitation Manufacturer may give technical reasons to the X marks PF One HAH AGUA gt The Environmentally Friendly Use Period EFUP was determined through experience AEP BADE AIS rh gt ASAE AEP ET 320074 gt BAGER AFERRA 03 AA gt BIA EE The date of Manufacture is in code within the serial number The first three numbers are the year of manufacture 207 is year 2007 followed by a letter for the month A is January B is February and so on o Pp H HL Series Hazmat Table 60 1263 072 Rev Radio Interference Statement FCC This equipment has been tested and found to comply with the limits for a class A digital device pursuant to Part 15 of the FCC Rules These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a commercial environment This equip ment generates uses
66. Syringe Pumps Section 1 Introduction Press softkey D and then softkey A to select pump A The display will automatically switch to the run screen and a will be displayed in the upper left corner Press the orange MENU key on the controller front panel Press number 1 to select UNITS Press number 3 to select PSI for the pressure units Press number 5 to select ML MIN for the flow units The first line of the display will show the selected units Press softkey D PREVIOUS to return to the main menu 8 Press softkey D RETURN to exit the main menu 9 Push the blue CONST FLOW key to set the pump mode to 10 constant flow CFa will be displayed in the upper left corner of the screen Press softkey A FLOW RATE The words ENTER FLOW RATE should flash on the display Use the numeric keys to enter 1 0 a flow rate of 10 ml min Press the ENTER key to load this setpoint M Note If you make an error press the orange CLEAR ENTRY key to delete it 11 12 Figure 1 10 Four pump stop menu Press the blue RUN key Observe the flow rate displayed on the first line After a few moments the setpoint and flow rate display should match Once the setpoint and flow rate match press the STOP key a If four pumps are connected to the controller you will be prompted to press A to stop pump A B to stop pump B C to stop pump C D to stop pump D or STOP to stop all pumps PRESS RUN KEY TO ESCAPE
67. TION RATE 0 01 ul min to 60 ml min for any pressure up to 551 5 bar For higher pressures see Figure 1 1 PRESSURE RANGE PRESSURE ACCURACY PRESSURE REPEATABILITY 0 6895 to 689 5 bar 0 5 of full scale at constant temperature 0 5 of full scale within 48 hours at constant temperature ZERO PRESSURE DRIFT 0 25 of full scale within 48 hours at constant temperature PRESSURE DISPLAY RESOLUTION 6 895 kPa AMBIENT TEMPERATURE RANGE 5 to 40 C TEMPERATURE DRIFT x 0 12 of full scale C HUMIDITY 9596 maximum CYLINDER CAPACITY 102 93 ml DEAD HEADSPACE VOLUME 1 30 0 020 ml POLLUTION DEGREE INSTALLATION CATEGORY MAXIMUM ALTITUDE Using water at 137 9 bar and a temperature controlled environment at 30 C b The analog output is an optional accessory c Pressure repeatability specification is based upon re zeroing pressure transducer every 48 hours Refer to sub section ZERO PRESS in Section 3 of the manual for re zeroing procedure d Volume in and above the piston seal head clearance at automatic shutoff and inlet and outlet ports to the fittings 1 2 HL Series Syringe Pumps Section 1 Introduction Table 1 2 260HL Technical Specifications POWER REQUIREMENTS see section 1 5 117 12 Vac 3 2 A maximum 234 23 Vac 1 6 A maximum Jractory Set LINE FREQUENCY 50 or 60 Hz LINE VOLTAGE NOISE TOLERANCE 1 7 x nominal rms li
68. TS If multiple pumps are connected to a single controller the SELECT PUMP option selects the pump A B C or D whose current state and settings appear on the display The letter of the current pump will appear in lower case in the upper left corner of the run screen When multiple pumps are connected the UNITS selected for one pump will apply to all of the pumps The refill option allows you to set the refill rate or set the pump to automatically refill when a certain volume is reached Press MENU gt REFILL 3 The auto refill menu will appear If more than one pump is connected to the controller pump B pump C and pump D will be displayed on the fourth line To select a pump press the softkey under the pump designation Press 1 to set the volume for pump A The units to the right of the symbol will blink indicating that you should enter a volume Use the number keys to enter an appropriate value and then press ENTER 1 Press 3 to set the refill rate for the designated pump The refill rate can also be changed from the main screen while the pump is refilling 2 message will blink on the screen prompting you to enter the selected refill rate 3 Enter the desired rate with the number keys press ENTER 4 To save and exit the refill menu press D PREVIOUS The first line will display OFF or ON indicating whether or not this feature is enabled for pump A or the currently selected pump Press 4 to
69. URES PLEASE UTILIZE APPROPRIATE TUBING AND CONNECTIONS AS NOTED IN THE MANUAL Reducing union AU Figure 2 5 Outlet valve package connection 2 4 3 In Line Filter Package The optional in line filter package should be used when it is important to filter flow exiting the pump This package contains a 0 5 um filter and Me tubing Table 2 4 Optional in line Filter Package 68 1247 011 Part Number Description 60 1243 231 1 5 m stainless steel tubing 146 OD x 0 020 ID 60 1243 232 0 3 m stainless steel tubing 46 OD x 0 020 ID 209 9012 17 In line solvent filter with replacement frit 0 5 micron filter 209 0094 07 Zero volume lA 6 valve nut 209 0094 08 Zero volume lA 6 valve ferrule Installation 1 Attach the reducing adapter from the pump accessory package to the pump outlet using the nut and ferrule pro vided Or connect to the reducing union of the outlet valve package 2 Attach the 16 1 5 m length of tubing to the reducing adapter Cut to the desired length 2 9 HL Series Syringe Pumps Section 2 Fluid System Connections amp Accessories 1 5 m Tubing Length Figure 2 6 In line filter package 2 5 Temperature and Pressure Controls 2 5 1 Cylinder Insulating Cover 2 10 3 Then attach the in line filter to the end of the tubing The flow direction is indicated on the filter body 4 Connect the remaining 0 3 m length of tubing betw
70. able 4 1 two kits required Table 4 1 Manual Refill Valve Kits Pump Model Part Number 1000HL 68 1247 117 500HL 68 1247 083 100HL 260HL 68 1247 077 4 1 HL Series Syringe Pumps Section 4 Gradient Pumping for Pressure Flow and Concentration Modes Table 4 2 Manual Outlet Valve Kits Pump Model Part Number 1000HL 68 1247 118 500HL 68 1247 082 100HL 260HL 68 1247 078 4 2 Connecting the The syringe pump has two ports at the top of the cylinder One System port is used as the inlet for filling the pump and the other as the outlet either port may be used as inlet or outlet Inlet and outlet connections to each pump must be made identically Standard plumbing connections vary between pump models See Table 4 3 for standard port information When making fluid connections that use ferrules be sure to use the ferrules supplied for that pump by Teledyne Isco Push the tubing completely into the connector and finger tighten Then tighten with a wrench to clamp the ferrules onto the tubing Table 4 3 Swaging Detail NUT 5 16 24 FERRULE mes 1 8 Valco a 1 2TURN PAST FINGER TIGHT FRONT FERRULE NUT5 16 24 1 g NPT 1 lt 3 4 TURN PAST SR PERDER et FINGER TIGHT Ya NP FRONT FERRULE er PAST T ck mi FINGER TIGHT BACK FERRULE M Note Pump models 260HL and 100HL have a direct connection as shown in Figu
71. al fashion i e the pump control cables are attached to the pump B and C connectors on the rear panel of the pump controller The cable you select to connect to your network will depend on the type of serial port your computer has and the number of controllers you wish to connect Network communications are initiated by your computer To connect one controller use a null modem cable available from Teledyne Isco part 4480 7996 00 to connect the 9 pin serial output port of the computer to the RS 232 C port on the rear of the controller as shown in Figure 7 1 7 3 HL Series Syringe Pumps Section 7 Serial Interface D SERIES NE CONTROLLER 4 CONTROL COMPUTER T D A 7 RS 232 C T e ER j e SYRINGE PUMP Null Modem Cable part 480 7996 00 Figure 7 1 Serial network connection example Single connection With this configuration your sample program is capable of Constant flow Constant pressure Gradient 7 4 2 Two Controllers To connect two controllers in the network use the Y cable Compiler required 68 1020 198 as shown in Figure 7 2 M Note In order for the network to operate properly all instruments connected to the network must be turned on even if they are not being used C PUMP 1 D SERIES D SERIES
72. and can radiate radio frequency energy and if not installed and used in accordance with the instruction manual may cause harmful interference to radio communications Operation of this equipment in a residential area is likely to cause harmful interference in which the user will be required to correct the interference at his own expense Canada This ISM apparatus meets all requirements of the Canadian Interference Causing Equipment Regulations Ce g n rateur de fr quence radio ISM respecte toutes les exigences du R glement sur le materiel brouilleur du Canada Teledyne Isco One Year Limited Factory Service Warranty This warranty exclusively covers Teledyne Isco instruments providing a one year limited warranty covering parts and labor Any instrument that fails during the warranty period due to faulty parts or workmanship will be repaired at the factory at no charge to the customer Teledyne Isco s exclusive liability is limited to repair or replacement of defective instruments Teledyne Isco is not liable for consequential damages Teledyne Isco will pay surface transportation charges both ways within the 48 contiguous United States if the instrument proves to be defective within 30 days of shipment Throughout the remainder of the warranty period the customer will pay to return the instrument to Teledyne Isco and Teledyne Isco will pay surface transportation to return the repaired instrument to the customer Teledyne Isco wi
73. ansmit Transmit Receive Receive 6 1 3 Modbus Configuration To access the Modbus configuration settings press Options gt Modbus Options 1 Modbus RTU 2 Modbus TCP 6 2 HL Series Syringe Pumps Section 6 Modbus Configuration Table 6 1 Modbus TCP IP Configuration Options Item Description IP Address Internet protocol address of device Default 192 168 1 200 Subnet Mask Subnet mask Default 255 255 255 0 Gateway Gateway Default 0 0 0 0 Port TCP port Default 502 Table 6 2 Modbus RTU Configuration Options Item Value Description 300 1200 2400 4800 9600 Communication Speed Default 19200 38400 57600 115200 19200 Slave ID 1 247 Device address Default 1 Even I Stop Bit Odd 1 Stop Bit None 1 Stop Bit None 2 Stop Bit Baud Rate Communication parameters Default Even 1 Stop Bit Word order for 32 bit integers and IEEE 754 floating point Word Order Big Endian Little Endian numbers Big Endian AB CD Little Endian CD AB Default Big Endian Time to transmit single charac Min Baud Rate ter t1 Used to calculate inter Character Time dependent frame and inter character Max 999ms space t1 5 13 5 50 m sec default Table 6 3 Supported Modbus Function Codes Function Code Description 01 Read Discrete Qutput Coils 03 Read Analog Output Holding Registers Write Single Discrete Output Coil Write Multiple Discrete Ou
74. aries depending on the mode the rate and units are set by the user so these will vary depending on your programming requirements The ending time will always be displayed on this line Ending Time Current Pressure 4 PS MIN FINAL PSI 00 00 00 Final Pressure Rate Line Four Line four varies depending on the mode The options presented on this line are softkey selectable i e you use the softkeys A D located under the screen to choose the option 3 2 3 2 4 Selecting Operating Parameters 3 3 Main Menus 3 4 Menu One HL Series Syringe Pumps Section 3 Basic Programming and Operation There are four ways to program the system from a menu screen Menu Selection The number keys are used to select from listed menu items Softkey Selection The softkey programming options appear on the fourth line of the screen To either toggle or select an option press the softkey A D directly below that option Keypad Selection The programming keys are located on the left side of the keypad The pump mode limits and refill are all options that may be selected from the keypad Value Selection The number keys are used to enter all numeric values required for pump operation When a numeric value is required a message will blink prompting you to enter an appropriate value There are four main menu screens Access Menu 1 by pressing MENU To move forward and back between Menus 1 2 3 and 4 or to return to the
75. at the valve 3 Connect the PTFE refill tubing with the filter to the other port of the valve using the nuts and ferrules supplied M Note When connecting to pressurized sources in supercritical fluid applications use the stainless steel tubing without a filter An in line filter is contained in the CO connection package refer to technical bulletin TBO8 CO Applications and Technical Notes at http www isco com sp_applications techbulletins Nut ferrule x To system Spacer block Valve PTFE tubing or stainles stgel tubing Figure 4 1 Pump inlet connections 4 4 Section 4 4 2 2 Outlet Connections Figure 4 2 Pump outlet connections HL Series Syringe Pumps Gradient Pumping for Pressure Flow and Concentration Modes Kit components and connections are shown in Figure 4 2 1 Mount the two way outlet valve on the side of the pump housing opposite the refill valve with the spacer block and screws provided 2 Connect the pre bent SST tubing between one port of the valve and the pump outlet Use the nut and ferrule to con nect the tubing at the outlet and the valve fittings to con nect the tubing at the valve 3 Connect the 5 1 cm length of l tubing to the other port of the valve using the valve fittings 4 Connect the 18 side of the reducing union to the tubing 5 Connect the l46 side of the reducing union to the 1 5 m length of 6 tubing This tubing may be cu
76. ate of Pump B Volume remaining in Pump B ml min ml hr ul min ul hr See NOTE 2 milliliters Actual pressure of Pump C Actual flow rate of Pump C ATM BAR kPa PSI See NOTE 1 ml min ml hr ul min ul hr See NOTE 2 Volume remaining in Pump C Actual pressure of Pump D milliliters ATM BAR kPa PSI See NOTE 1 Actual flow rate of Pump D ml min ml hr ul min ul hr See NOTE 2 Volume remaining in Pump D milliliters System flow rate of multi pump pair AB ml min ml hr ul min ul hr See NOTE 2 System pressure of multi pump pair AB ATM BAR kPa PSI See NOTE 1 Total volume delivered by multi pump pair AB Liters System flow rate of multi pump pair CD ml min ml hr ul min ul hr See NOTE 2 System pressure of multi pump pair CD ATM BAR kPa PSI See NOTE 1 Total volume delivered by multi pump pair CD Liters Analog voltage input on Analog Input 1 of the accessory connector Volts Analog voltage input on Analog Input 2 of the accessory connector Analog voltage input on Analog Input 3 of the accessory connector Volts 6 9 HL Series Syringe Pumps Section 6 Modbus Configuration Table 6 6 Holding Registers Continued of Registers Register Description Analog voltage input on Analog Input 4 of the accessory connector Volume at which Pump A will automatically switch to r
77. automatic rapid pressurization cycle Constant Flow mode only for Pump B 1 Activates the automatic rapid pressurization cycle Constant Flow mode only for Pump C E 1 Activates the automatic rapid pressurization cycle Constant Flow mode only for Pump D 1 Pump A is Equilibrating 1 Pump B is Equilibrating 1 Pump C is Equilibrating 1 Pump D is Equilibrating 1 Pump A is in Hold clock stopped 1 Pump B is in Hold clock stopped 1 Pump C is in Hold clock stopped 1 Pump D is in Hold clock stopped 1 Pump A amp Pump B in Concentration Gradient 1 Pump A in Pressure Gradient R R R R R R R R R R R 1 Pump A in Flow Gradient 1 Controller in Local Operation 1 Controller in Remote Operation 1 Controller in External Operation 1 External Refill control enabled 1 Zero pressure sensor offset for Pump A 1 Zero pressure sensor offset for Pump B 1 Zero pressure sensor offset for Pump C 1 Zero pressure sensor offset for Pump D 0 Pressure Integrator for Pump A OFF 1 Pressure Integrator for Pump A ON 0 Pressure Integrator for Pump B OFF 1 Pressure Integrator for Pump B ON E 0 Pressure Integrator for Pump C OFF 1 Pressure Integrator for Pump C ON 0 Pressure Integrator for Pump D OFF 1 Pressure Integrator for Pump D ON 0 Pressure Deadban
78. ctor lessen 7 3 Example of BASIC program to demonstrate conversion of pump commands to DASNET frames sesssss 7 8 Example of C program to demonstrate conversion of pump commands to DASNET frames 20 0 000 7 9 Serial Commands vester er y or ee in a 7 15 Error Messages cU VR RE CIERRAN ue ONERE PE 1 29
79. d for Pump A OFF 1 Pressure Deadband for Pump A ON 0 Pressure Deadband for Pump B OFF 1 Pressure Deadband for Pump B ON 0 Pressure Deadband for Pump C OFF 1 Pressure Deadband for Pump C ON 0 Pressure Deadband for Pump D OFF 1 Pressure Deadband for Pump D ON 0 AUTO REFILLAOFF 1 AUTO REFILL A ON 0 AUTO REFILL B OFF 1 AUTO REFILL B ON 6 5 HL Series Syringe Pumps Section 6 Modbus Configuration Table 6 5 Coils Continued Register Address Type Description 0 AUTO REFILLC OFF 1 AUTO REFILL C ON 0 AUTO REFILL D OFF 1 AUTO REFILL D ON 0 AUTO FILL A OFF 1 AUTO FILL A ON 0 AUTO FILL B OFF 1 AUTO FILL B ON 0 AUTO FILL C OFF 1 AUTO FILL C ON 0 AUTO FILL D OFF 1 AUTO FILL D ON 0 DIGITAL OUTPUT BIT 1 HIGH 1 DIGITAL OUTPUT BIT 1 LOW 0 DIGITAL OUTPUT BIT 2 HIGH 1 DIGITAL OUTPUT BIT 2 LOW 0 DIGITAL OUTPUT BIT 3 HIGH 1 DIGITAL OUTPUT BIT 3 LOW 0 DIGITAL OUTPUT BIT 4 HIGH 1 DIGITAL OUTPUT BIT 4 LOW 0 DIGITAL OUTPUT BIT 5 HIGH 1 DIGITAL OUTPUT BIT 5 LOW 0 DIGITAL OUTPUT BIT 6 HIGH 1 DIGITAL OUTPUT BIT 6 LOW 0 DIGITAL OUTPUT BIT 7 HIGH 1 DIGITAL OUTPUT BIT 7 LOW 0 DIGITAL OUTPUT BIT 8 HIGH 1 DIGITAL OUTPUT BIT 8 LOW 0 Disable Dispense Mode A 1 Enable Dispense Mode A Only configure if in Constant Flow Mode 0 Disable Dispense Mode B 1 Enable Dispense Mode B Only configure if in Constant Flow Mode 0 Disable Dispense Mode C 1 Enable Dispen
80. der and select Universal D Series Pump driver to download the utility to a zip folder Extract the sub folder named Remote Pump 7 7 HL Series Syringe Pumps Section 7 Serial Interface Table 7 3 Example of BASIC program to demonstrate conversion of pump commands to DASNET frames 1 CLS Z PRINT INPUT ALL ENTRIES IN CAPITAL LETTERS 5 INPUT INPUT UNIT ID gt gt UNITNUM GET UNIT NUMBER 30 REM OPEN COM PORT SET FOR COM2 EDIT TO COMI IF NEED 40 OPEN COM2 1200 N 8 1 ASC FOR RANDOM AS 2 50 PRINT INPUT ENTER STRING HIT Q TO EXIT gt gt I GET OUTPUT STRING FROM USER 65 IF I THEN GOTO 50 IF BLANK INPUT THEN GO BACK 70 IF I Q THEN GOTO 200 IF Q INPUT THEN QUIT 80 GOSUB 10000 CONVERT STRING TO DASNET FORMAT 90 GOSUB 20000 OUT DASNET STRING TO COM 2 120 PRINT DASNET FORMATTED CMD gt gt O OUTPUT FORMATTED CMD TO USER 124 PRINT PRINT gt gt gt gt PRESS CTRL C IF NO RESPONSE FROM PUMP 125 LINE INPUT 2 Z GET RESPONSE FROM PUMP CR ENDS STRING 127 PRINT DASNET RESPONSE gt gt Z PRINT RESPONSE 128 Z CLEAR BUFFER 130 GOTO 50 GO BACK 200 CLOSE 2 CLOSE COM2 210 SYSTEM END PROGRAM AND EXIT TO DOS 10000 REM this SUBROUTINE will convert a string I into a string O 10005 REM in DASNET protocol 10015 REM UNITNUM UNIT NUMBER OF PUMP 10020 REM AFTER THE STRING IS SENT TO PUMP A CR IS REQUIRED TO TERMINATE MESSAGE 10030 REM VAR USED 0 1 IL Y
81. e Top 0 Bottom 1 Cylinder is full Top 1 Bottom 0 Cylinder is empty Top 1 Bottom 1 Illegal state An illegal state can indicate failure of one or both sensors The controller display will toggle immediately between CYLINDER FULL and CYLINDER EMPTY when you press REFILL or RUN the pump will not run Flag Sensor Figure 8 3 Optical sensor interrupted by flag bottom sensor shown rear tower cover plate removed Select the pump to be tested A B or C TRAVEL LIMIT SENSE AND MOTOR POSITION Upper limit 0 Lower limit 0 Tach counter Up 100 Down 100 Exit Press UP 100 A or DOWN 100 B to move the piston up or down 100 counts The tach counter value will increase or decrease by 100 counts accordingly When the ball nut assembly reaches the top or bottom of the tower the corresponding limit will change from 0 to 1 If the cylinder was refilled using the REFILL button 8 5 HL Series Syringe Pumps Section 8 Pump Maintenance Troubleshooting and Servicing it may require multiple presses of the UP 100 softkey before the Lower Limit indicator changes to 0 When the Lower Limit indi cator is 1 pressing the DOWN 100 softkey will have no effect If the rear tower cover plate is removed to expose the sensors as shown above in Figure 8 3 you can simply interrupt the sensors with a slip of paper rather than using the flag If either sensor fails the sensor harness must be replac
82. e ball screw down its entire length The precision thrust bearing at the base of the ball screw refer to Figure 8 7 is factory lubricated and should not need re greasing 8 9 HL Series Syringe Pumps Section 8 Pump Maintenance Troubleshooting and Servicing 8 7 Flushing the Cylinder 8 7 1 Gas Solvent Changeover 8 7 2 Liquid Solvent 8 10 Changeover and Flushing To clean the cylinder After cylinder seal maintenance or during modifier liquid solvent change the pump cylinder should be flushed to remove possible residue The way in which the cylinder is flushed will depend on your pumping system and whether you are pumping a liquefied gas such as CO see Section 8 7 1 or a liquid modifier such as methanol see Section 8 7 2 This procedure is used when changing from one gaseous solvent to another 1 Close the valve on the fluid supply tank so that no solvent is supplied to the system 2 Turn the controller ON Run the pump until the cylinder is empty If there was any pressure in the system wait until all the pressure bleeds off Change the solvent tank en Close the pump outlet valve Open the valve on the tank to repressurize the system Cycle the pump between REFILL and RUN a few times opening and closing the appropriate valves at the proper time to purge any remaining gas from the pump Use about 10 20 mls per stroke to purge ao oO e This procedure is typically used for modifier sys
83. e setpoint Returns the minimum pressure setpoint MODE MODE A P B P C P D P A B C D refer to the pump connection P Constance Pressure F Constant Flow R Refill PG Pressure Gradient F1 Flow Gradient 1 Pump F2 Concentrated Gradient 2 Pump CF Continuous Constant Flow CP Continuous Constant Pressure MO Modifier Mode 2 Pump MM Modifier Mode 3 Pump MODIFIER Put pumps in modifier addition mode PRESS PRESSB PRESSC PRESSD Enter to designate pressure setpoint constant pressure mode PRESS PRESSCD Returns the delivering pump pressure in continuous pumping mode and modifier addition mode In INDEPENDENT mode PRESS returns the pump A pressure and PRESSCD returns the pump C pressure PRESSA PRESSB PRESSC PRESSD Returns the actual pressure of the pump PRESSCNTRLDIFF1 7 18 Sets the pressure control input to Analog input 1 with a pressure range of 50 psi HL Series Syringe Pumps Section 7 Serial Interface Table 7 5 Serial Commands Continued Command Description PRESSCNTRLDIFF1 XXXXX Sets the pressure control input to Analog input 1 and sets the pressure range The range is 1 to 5000 the units are psi with a value of 5000 representing 5000 psi at 5 volts PRESSCNTRLDIFF2 Sets the pressure control input to Analog input 2 with a pressure range of 500 psi at 5 volts PRESSCNTRLDIFF3 Sets the pressure control input to Analog input
84. ed Press EXIT D to return to the diagnostic menu 8 4 5 SERIAL TEST Tests the serial channel First confirm operation of the internal port On the RS 232 C port on the rear of the controller short pins 2 and 3 and then run the test The screen should display Rec Serial Test Sent Serial Test Press any key to exit The four digits in the REC line should match the four digits in the SENT line with a very slight delay If the unit passes the internal port test next perform the I O test Use a null modem cable available from Teledyne Isco part 480 7996 00 to connect the 9 pin serial output port of a com puter to the RS 232 C port as shown on the following page D SERIES No CONTROLLER k CONTROL COMPUTER TI E E T DT a e RS 232 C Lg G CJ ES C q Null Modem Cable SYRINGE PUMP part 4480 7996 00 Figure 8 4 Serial connection to a computer Open a terminal emulator program such as HyperTerminal OHilgraeve Inc Monroe MI The factory default port settings are BPS 9600 Baud rate should match controller setting refer to Section 7 2 Data bits 8 8 6 8 5 Removing the Case Top 8 5 1 Controller Case Top Removal HL Series Syringe Pumps Section 8 Pump Maintenance Troubleshooting and Servicing Parity None Stop bits 1 Flow control None Run the t
85. ed in Table 1 8 and shown in Figure 1 7 fr PE ISCO Figure 1 5 Pump controller key functions Table 1 5 Pump Controller Front Panel Label Item No on Figure 1 5 Connector Description Programming keypad Used to program controller Softkeys Labeled A B C and D used to select menu items displayed directly above them Liquid crystal display 40 Characters x 4 line A B C D HL Series Syringe Pumps Section 1 Introduction Table 1 6 Pump Controller Key Functions Description Softkeys used to select displayed options PRGM GRAD Puts the pump in gradient mode and accesses the softkey driven gradient programming CONST PRESS Constant pressure Puts pump in constant pressure mode CONST FLOW Constant flow Puts pump in constant flow rate mode STORE Stores the current program in nonvolatile memory and exits program ming mode LIMITS Displays and allows changes to the maximum and minimum pressure and flow rate limits RAPID PRESS RECALL Rapid pressure Allows rapid pressurization to the stable pressure point and then switches automatically to constant flow Available in constant flow mode only NOTE This feature is automatic i e RAPID PRESS is pressed only once and the user does not enter a pressure although entering a target pressure may speed equilibration Replaces the current program with one recalled from nonvolatile memory
86. een teen eens 2 14 3 1 Menu 1 program selections 0 ccc eee teens 3 3 2 2 Units menu Gee Ore ee ESE Nae tied ae tosh deb eal TUNE 3 3 3 3 Menu 2 program selections 0 ccc eee eee n nes 3 5 3 4 Serial option Menu 3 6 3 5 Total volume reset 0 e ei EE ehh 3 6 3 6 Menu 3 program selections 0 ccc cee e eens 3 7 3 7 Location of transducer midpoint value 0 00 eens 3 8 3 8 Limits ment u eee eh ai ae ia eee Le abe asd s yk SRA Sete 3 10 3 9 Limits setpoint Max Press menu 0 00 cece tee eens 3 10 3 10 Depiction of dispense mode sequence 0 0 eee eee cence ees 3 16 3 11 External control menu 0 T ee eee tence eee 3 18 3 12 Button sequence for external control setup 00 cee eee ees 3 19 4 1 Pump inlet connections 0 0 cc hr 4 4 4 2 Pump outlet connections 0 ccc teen eee eens 4 5 5 1 Air valve installation for 100HLf and 260HLf pumps 2000 5 3 5 2 Air valve installation for 5OOHLg 0 0 0000 5 4 5 3 Air valve installation for 1000HLer pump 0 rare e 5 5 5 4 Keystrokes to specify valve type 0 0 00 cece raneren rar ra nr 5 9 5 5 Keystrokes to reset volume totalizer oo oooooooooooorrornmo oo o 5 9 5 6 Keystrokes to set up constant flow 0 cece eee nee 5 10 5 7 Keystrokes to set up constant pressure 0 0 0 cee cece ees 5 10 5 8 Keystrokes to Run or Stop the pu
87. een the in line filter and the receiving device Cut to the desired length 0 3 m Tubing Length In line Filter Some applications such as those with very low flows may require additional measures to maintain steady rates The optional insulating cover can reduce flow noise due to tem perature fluctuations and improve pump performance at flow rates under 500 pl min The insulating cover is not available for Model 1000HL Part numbers e Insulating cover for 100HL and 260HLr 60 1267 003 Insulating cover for 500HL 60 1267 004 The cover consists of two pieces that fit over the cylinder as shown in Figure 2 7 HL Series Syringe Pumps Section 2 Fluid System Connections amp Accessories Figure 2 7 Cylinder insulation cover Notches in the cover provide openings for the inlet and outlet tubing and for the cable to the pressure transducer Installation 1 Install the back cover 2 Route the cable around tubing through the appropriate tabs 3 Install the front cover and secure the side latches 2 5 2 Temperature Control The optional cylinder temperature jacket assists in maintaining Jacket cylinder temperatures 30 to 100 C by circulating liquids such as water or water ethylene glycol solution through the 4 upper and lower hose connectors HL Series Syringe Pumps Section 2 Fluid System Connections amp Accessories CO connection kit CO 2 LI
88. efill mode if AUTO REFILL A is ON Volume at which Pump B will automatically switch to refill mode if AUTO REFILL B is ON Volume at which Pump C will automatically switch to refill mode if AUTO REFILL C is ON Volume at which Pump D will automatically switch to refill mode if AUTO REFILL D is ON Volume that Pump A will be refill to if AUTO LLTO Ais ON olume that Pump B will be refill to if AUTO LLTO B is ON F V F Volume that Pump C will be refill to if AUTO F V F LLTO C is ON olume that Pump D will be refill to if AUTO LLTO D is ON Percentage of total pump volume that the refilling pump of multi pump pair AB will refill to Percentage of total pump volume that the delivering pump of multi pump pair AB will deliver to Percentage of total pump volume that the refilling pump of multi pump pair CD will refill to Percentage of total pump volume that the delivering pump of multi pump pair CD will deliver to LID 10 20 30 40 50 60 70 80 90 D 10 20 30 40 50 60 70 80 90 D 10 20 30 40 50 60 70 80 90 D ENTRYS 10 20 30 40 50 60 70 80 90 POOR FILL MARK A POOR FILL MARK B POOR FILL MARK C POOR FILL MARK D Volume being dispensed by Pump A in Dis milliliters pense mode Volume being dispensed by Pump B in Dis illiliters pense mode Volume being dispensed by Pump C in Dis illiliter
89. el of the controller For any terminal shorted to ground the value should read a steady zero A XX XXXX ACC1 XX XXXX B XX XXXX ACC2 XX XXXX C XX XXXX ACC3 XX XXXX D XX XXXX ACC4 XX XXXX MORE ACCESSORY DIGITAL DIGITAL OUTPUT DIGITAL INPUT GROUND A B C D 1234 12 3 415617 8 22220010 20 20 22220000 0 0 0 0 Gnd Figure 8 2 Analog Input diagnostic screen Press EXIT D to return to the diagnostic menu 8 4 HL Series Syringe Pumps Section 8 Pump Maintenance Troubleshooting and Servicing 8 4 3 KEYPAD TEST The screen will briefly display the name of each key pressed Press EXIT D to return to the diagnostic menu 8 4 4 MOTOR Tests the digital position controls CONTROL LIMITS From the Limits menu you can set minimum and maximum flow rate limits and pressure limits as well as the maximum flow rate in constant pressure mode High and low limits cannot exceed pump specifications The pump stays within these limits by means of a flag that moves up and down with the ball nut assembly and two optical sensors at the top and bottom of the tower side plate When a sensor is interrupted it has a logic 1 when it is uninter rupted it has a logic 0 Therefor
90. erface 7 30 HL Series Syringe Pumps Section 8 Pump Maintenance Troubleshooting and Servicing AN NDANGER RISK OF ELECTRIC SHOCK DISCONNECT THE ELECTRIC POWER BEFORE SERVICING ONLY TRAINED SERVICE PERSONNEL MAY REMOVE THE CASE TOP AN DANGER RISQUE DE CHOC ELECTRIQUE COUPER L ALIMENTATION AVANT LA R PARATION L USAGER NE DOIT PAS D MONTER L INSTRUMENT OU D RANGER LE M CANISME DEDANS ADRESSER LA REPARATION SEULEMENT AUX TECHNICIENS COMP TENTS A Ay WARNING Only trained service personnel may perform service work on this equipment Failure to comply will result in serious risk to life and limb and will void the hazardous locations certification A AN WARNING Earth ground bonding conductor Do not remove or disconnect Mise la terre Ne pas enlever ni d connecter A AN WARNING Line voltage is present inside this unit at all times regardless of switch settings If internal adjustments or repairs are necessary the line cords must be disconnected to remove possible shock hazard before opening the case co L HL Series Syringe Pumps Section 8 Pump Maintenance Troubleshooting and Servicing 8 1 Introduction 8 2 8 1 1 Service Department 8 1 2 How to Ship Returns The following sections contain maintenance and repair proce dures which you can do yourself or have done by a technician at your site To view the schematic drawings referred to in this section first find the serial
91. est again The screen should display Rec whatever is typed from PC Sent Serial Test Press any key to exit Anything typed on the computer keyboard should appear in the top line on the controller display The computer should contin ually display the serial test shown in the second line of the con troller display For some maintenance procedures the case top of the controller or the pump may need to be removed Because interior access is easier with the controller this method of troubleshooting should be used whenever possible AN DANGER RISK OF ELECTRIC SHOCK DISCONNECT THE ELECTRIC POWER BEFORE SERVICING ONLY TRAINED SERVICE PERSONNEL MAY REMOVE THE CASE TOP AN NDANGER RISQUE DE CHOC ELECTRIQUE COUPER L ALIMENTATION AVANT LA R PARATION L USAGER NE DOIT PAS D MONTER L INSTRUMENT OU D RANGER LE M CANISME DEDANS ADRESSER LA REPARATION SEULEMENT AUX TECHNICIENS COMP TENTS Troubleshooting for a number of issues can be done on the con troller main circuit board Remove the four screws holding the case top in place two screws on each side Lift the cover straight up and off 8 7 HL Series Syringe Pumps Section 8 Pump Maintenance Troubleshooting and Servicing Figure 8 5 Controller case top screws 2 of 4 shown 8 5 2 Pump Case Top Some maintenance and troubleshooting procedures require Removal accessing the pump module interior Remove the six screws holding the case t
92. extends beyond the ferrule to allow for proper crimping e Ifthe connection leaks retighten fittings Push the tubing completely into the port before tight ening the nut When connections are made to the cylinder cap the pressure reading may be affected If the pressure no longer reads zero release all pressure inside the pump then press ZERO PRESSURE on the front panel of the controller to readjust The tubing must be cut squarely to prevent possible problems Square ends are easier to insert through the ferrule and will decrease dead volume Electrochemically machined steel tubing should be used throughout the plumbing system Electrochemically machined tubing has flat burr free ends and is free of cutting residues This tubing is available pre cut through many chromatographic supply distributors in assorted lengths A less desirable alternative is to purchase a tubing cutter designed to handle steel tubing For quick fixes the tubing may also be cut by hand 2 3 3 Accessory Packages Installation 2 3 4 Draining Overflow HL Series Syringe Pumps Section 2 Fluid System Connections amp Accessories AN CAUTION Wear goggles to perform the following procedure Tools required Fine jewelers file goggles and two pairs of pliers 1 Using the jewelers file score the tubing around its entire circumference 2 Secure the tubing with pliers on either side of the score line with approximately 1 5mm between each
93. fers to check valves Volume totalizer The total volume delivered is displayed in liters at the top right corner of the screen Refer to the figure below to reset the volume totalizer to zero 1 AB Pumps 2 CD Pumps 3 AB amp CD Pumps V TOTAL VOLUME RESET ES PREVIOUS Press 2x to return to MORE run screen Figure 5 5 Keystrokes to reset volume totalizer 5 4 2 Constant Flow Mode Continuous flow mode is found under the multi pump options on Menu 2 Once you have accessed the multi pump options you may select Constant Flow mode or Constant Pressure mode 5 9 HL Series Syringe Pumps Section 5 Continuous Flow Introduction Installation amp Operation mew EB V CONTIN CONST FLOW 5 T will blink MORE PREVIOUS Starts Press 3x to operation return to run MULTI PUMP screen 1 AB Pumps 2 CD Pumps Figure 5 6 Keystrokes to set up constant flow 5 4 3 Constant Pressure Continuous flow mode is found under the multi pump options on Mode Menu 2 Once you have accessed the multi pump options you may select Constant Flow mode or Constant Pressure mode VES CONTIN CONST PRESS a 2 will blink PREVIOUS 0 Press 3x to Starts l return to run operation MULTI PUMP i screen 1 AB Pumps 2 CD Pumps Figure 5 7 Keystrokes to set up constant pressure 5 10 HL Series Syringe Pumps Section 5 Continuous Flow Introduction Installation amp Operation
94. g control or input wire should be connected to terminal 2 under ANALOG INPUT If three pumps are used with the controller the third analog control or input wire should be connected to ter minal 3 under ANALOG INPUT If four pumps are used with the controller the fourth analog control or input wire should be connected to terminal 4 under ANALOG INPUT When using one of the multi pump operation modes only the ANALOG INPUT terminal 1 needs to be connected to control the A B pump pair and ANALOG INPUT terminal 3 needs to be con nected to control the C D pair Before programming the controller to accept the analog signal you must know the maximum flow rate constant flow or the maximum pressure constant pressure 3 17 HL Series Syringe Pumps Section 3 Basic Programming and Operation 3 12 3 External Control for Refill Figure 3 11 External control menu 3 18 M Note The high and low limits you enter cannot exceed the pump specifications Normal input 10V To determine the analog voltage range use the formula explained below FSx 5 Vmax where FS Maximum flow rate or Maximum pressure Full Scale This is the same value entered when setting LIMITS as described in Section 3 of your user manual for MAX PRESS or MAX FLOW V Volts per unit of flow or pressure U Incremental unit of flow or pressure Vmax Maximum input voltage The refill option allows you to set the refill rate or set the pump
95. g and Operation Figure 3 7 Location of transducer midpoint value 3 8 3 6 4 Diff Modes 3 6 5 Press Integral 3 6 6 Pressure Deadband Press CALA CALB CALC or CALD to select the correct pump enter the calibration number and press ENTER M Note This calibration feature is not applicable to HL model pumps The midpoint adjustment value is individual to each pump and is stored within the controller software Therefore this value must be re entered if the controller or pump is switched con troller is reset or if a pump is plugged into a different port on the controller This feature allows the use of other analog inputs for pressure input to the pump The standard pressure transducer MUST be connected to pump in order for this feature to work Press 1 OFF to turn off this feature Press 2 0 to 50 ANLG1 to use a 5 volt 50 psi transducer on ANALOG INPUT 1 of the ACCESSORY connector Press 3 Custom ANLGI1 to use 5 volt custom pressure transducer on ANALOG INPUT 1 of the ACCESSORY connector Press 4 500 ANLG2 to use a 5 volt 500 psi pressure transducer on ANALOG INPUT 2 of the ACCESSORY connector Press 5 5000 ANLG3 to use a 5 volt 5000 psi transducer on ANALOG INPUT 3 of the ACCESSORY connector If the pressure control appears to be unstable or incorrect toggle this feature on by pressing 1 2 3 or 4 for the correct pump this activates a proportional integral derivative PID controller which m
96. ge format are in section 7 8 The computer will be unit 0 and the pump will be unit 6 Network Controller and Master Unit 0 CRJIR 5D CR M Note A CR must start the network The controller is checking for the presence of unit 1 but will get no response in 200 ms because there is no unit 1 Network Controller and Master Unit 0 IR 5D CR Still no response Network Controller and Master Unit 0 IR 5D CR Still no response so unit 1 will be dropped from the poll HL Series Syringe Pumps Section 7 Serial Interface Network Controller and Master Unit 40 2R 5C CR Checks for unit 2 but will get no response in 200 ms because there is no unit 2 Network Controller and Master Unit 0 2R 5C CR Still no response Network Controller and Master Unit 0 2R 5C CR Still no response so unit 2 will be dropped from the poll In this way units 3 5 will be checked and dropped from the poll Network Controller and Master Unit 0 6R 58 CR Check for presence of unit 6 Unit 6 R 8E CR Unit 6 responds Network Controller and Master Unit 0 7R 57 CR Since unit 7 does not exist it will be dropped from the polling scheme Network Controller and Master Unit 0 6ROO8IDENTIFY 84 CR The master verifies the fact that unit 6 is a Model D In this example the master and the network controller are a single unit If they were separate units the master would send the inquiry to the network controller then the networ
97. ghten the tubing nuts 6 Connect the inlet tube to the supply reservoir Connect the outlet tee to your apparatus HL Series Syringe Pumps Section 5 Continuous Flow Introduction Installation amp Operation Outlet 4 Pressure Transducer Cap 1 8 Valco Nut lt 1 8 Valco Ferrule 5 O Pre swaged s PR o 3 1 8 Valco Pe 1139 Connector al Tubing L e e Check Valve Actuator IT Inlet LJ ACCEBSORY DIGITAL 15 DIGITAL DIGITAL AMG INPUT GROUND OUTPUT gt B D Air Supply 1234 34 56 6 Panhead screws 2 Wire pair conversion table for A B Pump Pair and C D Pump Pair A BPump Digital C DPump Digital Wire Pair Output 1 gt Wires Wire Pair Output 15 Wires A M 15 v C6 15 B3 B3 15 B3 D7 15 B4 B4 15 B4 D8 15 Figure 5 1 Air valve installation for 100HLrand 260HL pumps 5 3 HL Series Syringe Pumps Section 5 Continuous Flow Introduction Installation amp Operation 1 8 Valco Nut Outlet lt a MT Pressure Transducer Cap B Pre swaged 1 8 8 1 8 Valco Ferrule o male connector tubing 9 P09 Oj tod bo Mounting lt a plate Check Valve Actuator e customer reservoir
98. he Return Merchandise Authori zation RMA specified Be sure to include a note describing the malfunction This will aid in the prompt repair and return of the equipment Teledyne Isco welcomes suggestions that would improve the information presented in this manual or enhance the operation of the equipment itself Teledyne Isco is continually improving its products and reserves the right to change product specifications replacement parts schematics and instructions without notice Contact Information Customer Service Phone 800 228 4373 USA Canada Mexico 402 464 0231 Outside North America Fax 402 465 3022 Email IscoCSR G teledyne com Technical Support Phone Toll Free 866 298 6174 Samplers Flow Meters and Multi parameter Probes Toll Free 800 775 2965 Syringe Pumps and Liquid Chromatography Email IscoService teledyne com Return equipment to 4700 Superior Street Lincoln NE 68504 1398 Other Correspondence Mail to P O Box 82531 Lincoln NE 68501 2531 Email IscoInfo teledyne com Revised April 2014 HL Series Syringe Pumps Table of Contents Section 1 Introduction 141 Introduction 2022 2 lene oe e eed Kr at Dia RE RU 1 1 1 2 Specifications o hoa ae ER eteren EG 1 1 13 Unpacking 2 sania naeh ead oe eee eee Vix 1 8 1 4 Controls and Indicators 0 a a verre rene nen eens 1 8 1 5 Electrical Connections 0 cece eee hh 1 13 1 6 Preliminary Checkout
99. ient mode any connected pumps not used for gradient are inoperable 1 To access the gradient programming menus press PRGM GRAD then DUAL SYSTEM GRADIENT 1 and CONTINUE C The home screen will appear with FG Flow Gradient in the upper left corner FGa 0 000mL MIN 2PSI 013 28mL STOPPED FILE 1 00 00 00 FINAL B PROGRAM EDIT REVIEW OPTION 2 Press PROGRAM A 3 At the prompt enter a file number between 1 and 99 This is the file name of your program and can be the name of a new program you are creating or a stored program you want to edit or run Press ENTER M Note If a selected stored program is in a different mode than that of the controller selected in Step 1 a brief notification will appear displaying the controller s mode and the file s mode If you attempt to run the program without changing the mode of either it or the controller the program will not run and the same message will be displayed 4 To enter the flow rate for this program either press FLOWRATE A and use the number keys and Enter or for maximum possible flow simply press MAX C ENTER FLOWRATE 0 00000 XX 00000 V FLOWRATE PREVIOUS MAX HL Series Syringe Pumps Section 4 Gradient Pumping for Pressure Flow and Concentration Modes 5 To proceed to the programming screen press STEP FWD B The programming screen will appear with the file number and step number at the top of the screen FGa FILE 1 STEP
100. ile 01 99 Figure 7 9 Gradient Upload Commands Single pump flow gradient 7 26 HL Series Syringe Pumps Section 7 Serial Interface Step Number 01 LGUL F xx S 01 FL xxx xxxx IB xx xx FB xx Xx D XXXX X l HI l Gradient Duration Range 0000 1 9999 0 minutes Final B Range 00 00 100 0 Initial B Range 00 00 100 0 Gradient Total Flow Rate Units milliliters minute Range 000 0000 Maximum Flow Rate Step Number 01 File 01 99 Step Numbers 02 through 99 LGUL F XX S9 xx IB xx XX FB xx xx D XXXX X Elk srl Gradient Duration Range 0000 1 9999 0 minutes Final B Range 00 00 100 0 Initial B Range 00 00 100 0 Step Number 02 99 File 01 99 Figure 7 10 Gradient Upload Commands Two pump flow gradient 7 27 HL Series Syringe Pumps Section 7 Serial Interface LGUL F xx S xx 1 xxxxx F xxxxx D xXxXxXxX X Loi XE Pressure Duration Range 0000 1 9999 0 minutes Final Pressure Range 00010 Maximum Units psi Initial Pressure Range 00010 Maximum Units psi Step Number 01 99 File 01 99 Figure 7 11 Upload Commands Single pump pressure programming 7 8 4 Range Command The RANGE command provides scaling information for the system parameters For example 6R006RANGEA34 may return a string similar to
101. ing or press softkey D to return to the previous screen To allow pump operation to be tailored to your application both the pressure and flow rate units may be set by the user refer to Section 3 4 1 The pump also allows user programmed refill as well as pumping rates refer to Section 3 4 3 The system protection limits may also be set by the user refer to Section 3 8 7 The programming screens are divided into separate menus These menus are accessed when different features are being pro grammed For complete information about programming menus refer to Sections 3 3 through 3 7 3 1 HL Series Syringe Pumps Section 3 Basic Programming and Operation 3 2 3 Run Screens The run screen appears once a program has been loaded and the pump is running The run screen which is determined by your program selections displays current information about pump operation The fol lowing sections explain the display line by line Line One No matter what mode you are in the first line of the run screen is always the same Active Pump Actual Pressure 1 PGa 000 mL MIN PSI XXX XX ml L_ Current Mode Volume Remaining Actual Flow Rate Line Two The second line varies slightly depending on the operating mode A file or step will only be displayed when oper ating in the gradient mode Elapsed Time E STOPPED FILE STEP 000 00 00 Step of program running or Status loaded file number Line Three Line three v
102. ing ACC CTRL then selecting each valve to open Zero the pressure in each pump by pressing ZERO PRESS and selecting each pump to zero Fill both pumps completely by pressing REFILL and select ing each pump to fill Pressurize both pumps by pressing RAPID PRESS Maximum flow rate and target pressure value will be displayed Press D to continue pressurization Reset total volume see Figure 5 5 must be full and delivering fluid Additional guidelines Please become familiar with the fol lowing guidelines Pressure limits for continuous constant flow mode are set by the limits of pump A for the A B pair and pump C for the C D pair Temperature changes can cause pressure fluctuations For available temperature control options contact Teledyne Isco For correct overpressure response shutdown must be set to ON under PUMP LIMIT options Enter the same refill rate separately for pumps A B C and D The refill rate should always be as high as possible to allow time for refill and repressurization before the next switchover HL Series Syringe Pumps Section 6 Modbus Configuration 6 1 Overview 6 1 1 Modbus RTU Modbus is a simple command response mechanism to read from and write to specific memory locations called registers register is a holding place for a piece of digital information within the equipment For more information on Modbus please refer to the following documents which can be found
103. ings Record your program settings and parameters before performing a reset operation If ZERO PRESS has been pressed all corrected offsets will be lost To perform a basic reset press MENU SYSTEM RESET 5 CON TINUE A To perform a hard reset 1 Ensure that power has been removed from the pump con troller 2 Press and hold the CLEAR ENTRY key on the front panel keypad 3 While holding the CLEAR ENTRY key apply power to the pump controller Keep the CLEAR ENTRY key pressed for one second 4 Release the key 8 3 HL Series Syringe Pumps Section 8 Pump Maintenance Troubleshooting and Servicing 8 4 Diagnostic Menu The syringe pump controller can run a number of system tests that are initialized using the keypad MORE MORE 2 DIAGNOSTIC MENU 1 LCDTEST 4 KEYPADTEST 2 ANALOGINPUTS 5 MOTOR CONTROL LIMITS 3 A DCAL 6 SERIALTEST MORE PREVIOUS Figure 8 1 Accessing the diagnostic menu 8 4 1 LCD TEST Cycles all segments of the display through all displayed char acters To stop the test press any key 8 4 2 ANALOG INPUTS Displays the values of all analog inputs in volts In the left column with all connected pumps at zero pressure the pressure readings should be near zero After the initial reading pressurize the pump and then observe the test screen once more to verify that the value for that pump has increased The right column represents the four analog input terminals on the rear pan
104. inimizes errors in the pressure control process This feature is on by default This feature dampens the corrective response to slight variations in pressure to reduce fluctuation If the pressure reading at the programmed setpoint appears to be unstable toggle this feature on by pressing 1 2 3 or 4 for the correct pump This feature is on by default 3 7 Menu Four 3 7 1 Modbus Options 3 8 Front Panel Keys 3 8 1 CONST PRESS and CONST FLOW 3 8 2 PRGM GRAD 3 8 3 HOLD 3 8 4 RECALL 3 8 5 STORE 3 8 6 REFILL 3 8 7 LIMITS HL Series Syringe Pumps Section 3 Basic Programming and Operation The Modbus RTU and Modbus TCP communications protocol is supported Complete information about using Modbus RTU and Modbus TCP communication is provided in Section 6 Modbus Configuration In addition to the menu options certain functions and modes are selectable from the front panel keyboard These keys place the system in constant pressure mode or con stant flow mode respectively Information for these operating modes can be found in Section 3 10 This key is used to place the pump system in gradient mode For complete information about gradient programming refer to Section 4 Gradient Pumping for Pressure Flow and Concen tration Modes The HOLD key is used while a gradient is running When HOLD is pressed the program clock freezes and current gradient parameters are maintained To continue the gradient pre
105. into on the rear panel of the pump controller To select a pump press the softkey under the pump desig nation The top line of the screen will indicate the currently selected pump The maximum and minium limits you set cannot exceed the pump specifications To set the limits 1 Press LIMITS To display the Limits menu enter the num ber of the limit you wish to program One of five limit set point menus will appear MAX PRESS MIN PRESS MAX FLOW MIN FLOW or FLOW LIMIT The MAX PRESS limit setpoint menu is shown in Figure 3 9 LIMITS CURRENT PUMP 1 MAX PRESS 3 MAX FLOW 2 MIN PRESS 4 MIN FLOW PUMP A 5 FLOW LIMIT PREVIOUS A B C D Figure 3 8 Limits menu MAX PRESS PSI SET PRESS VALUE ON ON OFF DISPLAY ALARM SHUTDOWN PREVIOUS A B C D Figure 3 9 Limits setpoint Max Press menu M Note These features are discussed later in this section under Limits Programming Options 2 Press the number 1 key to set the value A message will blink on the right side of the screen prompting you to enter the selected limit 3 Enter the desired limit setpoint using the number keys 4 Press ENTER to save the value The pump can be set to shut off or not at this limit condition by pressing softkey C under shutdown This will toggle this option to ON 5 To exit the limit menu press the softkey D PREVIOUS If four pumps are connected press STORE RECALL or CLEAR ENTRY to exit the limits menu
106. ion 2 Fluid System Connections amp Accessories Figure 2 1 Drain tube installation 2 4 2 3 5 Cylinder Washing Wash Gland standard feature see the following section for details The wash gland tubes can also be used as lines for draining away fluid Drip tray outlet A thin film of liquid wets the inside of the cylinder each time the piston travels up the cylinder The lowest flow rates are con ducive to the most abrasive or corrosive pumping environments as the deposited film remains on the inside of the cylinder wall for the longest time The Model 1000HL syringe pump is equipped for cylinder washing with two Ys tubes on the back to feed and drain the wash gland as shown in Figure 2 2 on the following page A small pump can be used to deliver the wash fluid through one of the two tubes to rinse the cylinder and seals The second tube drains the wash fluid to waste If the system is configured to recirculate the wash fluid ensure that you change the wash fluid at regular intervals Select a wash fluid that will best flush the cylinder of any residue left by the pumped fluid yet will not damage the seals A CAUTION The pressure in the wash gland and line should NEVER exceed the system pressure or the wash pressure limit as labeled on the pump Units with a wash pressure limit label can be damaged if the pressure is exceeded M Note If the primary pump seal fails the pressure of the delivery fluid will be e
107. k controller would send the message to the slave unit the next time it is polled The slave would respond with the message to the network controller The next time the master is polled the message would be relayed The same sequence would occur with all messages Since the master and the network controller are combined in this example the relaying of messages is not necessary Unit 6 RO27SERIES 1240 02__ Model _ _D PUMP REV XX CR The pump responds with identity and software revision letter In this example 02__ would be 021 Model D would be 260D REV __ signifies the software revision XX would be replaced by the correct checksum which is B4 Network Controller and Master Unit 1 6RO06REMOTEI6 CR This places the pump in the remote mode Unit 6 R 8E CR The pump acknowledges that it accepted the command 7 13 HL Series Syringe Pumps Section 7 Serial Interface 7 14 Network Controller and Master Unit 1 6ROOACONST FLOWFS CR This puts the pump into constant flow rate mode Unit 6 R 8E CR The pump verifies that it received the message Network Controller and Master Unit 1 6R009FLOW 1 00AB CR This sets the pump s flow rate to 1 00 ml per minute Unit 6 R 8E CR The pump verifies that it received the message Network Controller and Master Unit 1 6R 58 CR Polls the pump Unit 1 R 8E CR Pump responds Network Controller and Master Unit 1 6RO03RUNFO CR The pump is started Unit 1 R 8E CR
108. l the Teledyne Isco Service Department for instructions Many problems can often be diagnosed and corrected over the phone or by e mail without returning the instrument to the factory Instruments needing factory repair should be packed carefully and shipped to the attention of the service department Small non fragile items can be sent by insured parcel post PLEASE BE SURE TO ENCLOSE A NOTE EXPLAINING THE PROBLEM Shipping Address Teledyne Isco Attention Repair Service 4700 Superior Street Lincoln NE 68504 USA Mailing Address Teledyne Isco PO Box 82531 Lincoln NE 68501 USA 800 775 2965 lab instruments 866 298 6174 samplers amp flow meters Sales amp General Information 800 228 4373 USA amp Canada Phone Repair service Fax 402 465 3001 Email October 11 2013 P N 60 1002 040 Rev H IscoService Qteledyne com lu TELEDYNE ISCO Everywhereyoulook 1 1 1 2 1 3 1 4 1 5 1 6 1 7 1 8 1 9 2 1 2 2 2 4 2 5 3 1 4 1 4 2 4 3 5 1 5 2 6 1 6 2 6 3 6 4 6 5 6 6 7 1 7 2 7 4 7 5 7 6 100HL Technical Specifications 0 eee varar erververen 1 2 260HLf Technical Specifications 0 cece eens 1 3 500HL Technical Specifications 0 0 0 eee ee eee 1 4 1000HL Technical Specifications 20 cece ee ee eee 1 5 Pump Controller Front Panel Label 0 0 ccc cece eens 1 8 Pump Controller Key Functions 0 1 9 Pump
109. lect the desired operating mode by pressing CONST PRESS or CONST FLOW eg or HU EX PRESS FLOW CP or CF and selected EXTERNAL pump are displayed i V 3 SET RANGE MORE Enter Vmax value y Figure 3 12 Button sequence for external control setup 3 13 Remote RUN STOP The HL Series syringe pump RUN STOP function can be exter nally controlled by a switch contact closure or TTL input The input voltage is 5 volts and is internally pulled high RUN The 3 19 HL Series Syringe Pumps Section 3 Basic Programming and Operation 3 13 1 Wire Connections 3 20 input is level sensitive must remain high for RUN or low for STOP and must be high for normal operation of serial RS 232 control To enable the remote RUN STOP feature press MENU gt MORE gt A gt EXTERNAL gt 3 gt DIGITAL A On Then press RUN or force the RUN STOP pin low to enable the pump Thereafter the RUN STOP pin will control operation Pressing STOP on the front panel will override the RUN STOP pin Ay WARNING Connecting devices to energized circuits may cause personal injury or property damage Power must be removed from the pump before connecting external devices Two wires are required for external RUN STOP control The digital common or ground wire should be connected to one of the four DIGITAL GROUND terminals of the ACCESSORY con nector on the controller rear panel The control wire should be connected to ter
110. ll Flow Rate Range 65 60 55 50 45 40 35 30 25 20 15 10 Refill Flow Rate ml min 0 50 100 150 200 250 300 350 400 450 500 550 600 650 700 Pressure bar Refill Flow Rate ml min Maximum Pressure bar 0 0001 to 50 689 5 50 to 60 100 Refill Flow Rate x 13 789 Figure 1 1 100HLf refill flow rate range 260HL Refill Flow Rate Range 110 100 90 80 70 60 50 40 30 Refill Flow Rate ml min 20 10 50 75 100 125 150 175 200 225 250 275 300 325 350 375 400 425 450 475 500 525 550 Pressure bar Refill Flow Rate ml min Maximum Pressure bar 0 001 to 64 517 1 64 to 107 156 2 Refill Flow Rate x 5 612 Figure 1 2 260HL refill flow rate range 1 6 HL Series Syringe Pumps Section 1 Introduction 500HL Refill Flow Rate Range 200 180 160 140 120 100 80 60 Refill Flow Rate ml min 40 20 0 20 40 60 80 100 120 140 160 180 200 220 240 260 Pressure bar Refill Flow Rate ml min Maximum Pressure bar 0 001 to 122 258 6 122 to 204 297 72 Refill Flow Rate x 1 471 Figure 1 3 500HLrrefill flow rate range 1000HL Refill Flow Rate Range 425 400 375 350 325 300 275 250 225 200 175 150 125 100 75 50 25 0 0 10 20 30
111. ll not pay air freight or customer s packing and crating charges This warranty does not cover loss damage or defects resulting from transportation between the customer s facility and the repair facility The warranty for any instrument 1s the one in effect on date of shipment The warranty period begins on the shipping date unless Teledyne Isco agrees in writing to a different date Excluded from this warranty are normal wear expendable items such as pH sensors charts ribbon lamps tubing and glassware fittings and wetted parts of valves and damage due to corrosion misuse accident or lack of proper maintenance This warranty does not cover products not sold under the Teledyne Isco trademark or for which any other warranty is specifically stated No item may be returned for warranty service without a return authorization number issued by Teledyne Isco This warranty is expressly in lieu of all other warranties and obligations and Teledyne Isco specifically disclaims any warranty of merchantability or fitness for a particular purpose The warrantor is Teledyne Isco 4700 Superior Lincoln NE 68504 U S A This warranty applies to the USA and countries where Teledyne Isco does not have an authorized dealer Customers in countries outside the USA where Teledyne Isco has an authorized dealer should contact their Teledyne Isco dealer for warranty service Before returning any instrument for repair please call fax or e mai
112. luid System Connections amp Accessories a For the 500HL pump screw the male adapter into the pump outlet 2 Connect the pre bent length of stainless steel tubing to the outlet port on the pump using the nut and ferrule Connect the other end to the top port on the valve using the valve fitting M Note For the 500HL this piece of tubing should be cut to the proper length for connection to your system Due to the wide variety of applications for this model fittings to connect the tubing to your system are not provided in the kit M Note When nuts are torqued to the cylinder cap ports the pressure reading may be affected If the pressure no longer reads zero release the pressure in the cylinder and press ZERO PRESS 3 Usethe valve fittings to attach stainless steel tubing to the top port of the two way valve a For models 100HLf and 260HL connect the reducing union to the other end of this tubing 4 Connect the stainless steel tubing between the valve s bot tom port and your apparatus Cut to the desired length 2 8 Nut ferrule SST tubing Screws SST tubing to system HL Series Syringe Pumps Section 2 Fluid System Connections amp Accessories Table 2 3 Manual Outlet Valve Kits 1000HL 68 1247 118 500HL 68 1247 082 To refill kit ZB assembly 100HL 260HL 68 1247 078 NDANGER Spacer block RISK OF INJURY THIS EQUIPMENT PRODUCES HAZARDOUS PRESS
113. m is run on a PC as is the commands entered at the keypad will be output on serial port 2 This code was written in QBasic version 4 5 QBasic is an integrated BASIC language interpreter created by Microsoft Corp Redmond WA included in MSDOS version 5 0 and later and in Windows 95 98 NT 3 x and NTA 7 8 HL Series Syringe Pumps Section 7 Serial Interface Table 7 4 Example of C program to demonstrate conversion of pump commands to DASNET frames include lt stdio h gt include lt conio h gt include b comm c int conv_das unsigned char in 256 0ut 256 buf 256 char unitnum main unsigned port int speed cputs ENTER UNIT ID scanf d amp unitnum cputs ENTER COM PORT scanf d amp port cputs ENTER BAUD RATE scanf d amp speed comm_open port speed in 0 50 cputs ALL ENTRIES IN CAPS n r while 1 edit to path needed a C example of DASNET serial control get parms open comm port cputs n rENTER STRING Q TO QUIT gt gt gt cgets in if in 2 Q break conv_das amp in 2 out convert string cputs nDASNET FORMATTED OUTPUT gt gt gt puts out comm_putc 0x0d dput out comm_putc 0x0d dgets buf cputs nDASNET RESPONSE gt gt gt puts buf comm_flush comm_close conv_das char in char out unsigned sum char c_ptr c_ptr out out unitnum 0x30 outt R
114. m pressur ization This can be done by selecting limit 5 FLOW LIMIT The FLOW LIMIT value is used as the upper range of flow rate during pressure control This limit is not the same as the MAX FLOW limit which is a threshold above when the pump is stopped or an alarm is activated as selected by the operator To save your changes and return to the main menu press softkey D PREVIOUS This option is available when operating in the constant flow mode and in the two pump concentration gradient mode It allows rapid pressurization to a stable pressure point and then HL Series Syringe Pumps Section 3 Basic Programming and Operation switches automatically to the constant flow setpoint This is helpful when you are operating at a low flow rate but wish to rapidly pressurize a solvent 1 Press CONST FLOW to put the pump in constant flow mode or enter a two pump concentration gradient 2 Press RAPID PRESS 3 The controller will display maximum flow rate and target pressure setting If these values are correct press D to con tinue rapid pressurization 4 If you know approximately what the pressure will be when the system is stable enter this value as a target pressure Press A and enter the desired pressure value This should shorten the time required to stabilize the system pressure 5 If you would like to limit the maximum flow rate during the rapid pressurization phase press B and enter the desired flow rate limit 6 Press
115. minal 1 under DIGITAL INPUT If an electri cally isolated relay is used one relay terminal should be con nected to digital ground and the other to terminal 1 under DIGITAL INPUT If two pumps are used with the controller the second control wire should be connected to terminal 2 under DIGITAL INPUT If three pumps are used with the controller the third control wire should be connected to terminal 3 under DIGITAL INPUT If four pumps are used with the controller the fourth control wire should be connected to terminal 4 under DIGITAL INPUT HL Series Syringe Pumps Section 4 Gradient Pumping for Pressure Flow and Concentration Modes 4 1 Introduction Gradient pumping is used in applications requiring time con trolled or rate controlled delivery of a specific volume at a set flow rate or pressure or a two pump flow concentration You can program the controller to increase or decrease pressure or flow during different steps within a single program by entering a specific value at the beginning and end of each step NDANGER RISK OF INJURY THE PRESSURE PRODUCED COULD BE 700 BAR PLEASE UTILIZE APPROPRIATE TUBING AND CONNECTIONS NOTED IN THE MANUAL Tools and Parts for Single Pump System Open end wrenches Wy he he Ye Manual Refill Valve Kit see Table 4 1 Manual Outlet Valve Kit see Table 4 2 Tools and Parts for Dual Pump System Open end wrenches Ya 46 he 37g Manual Refill Valve Kit see T
116. mps 0 ce cee eee eens 5 11 6 1 RS 485 Connection 0 0 ec eee en mmm 6 2 7 1 Serial network connection example Single connection 7 4 7 2 Serial network connection example Dual connection 05 7 4 HL Series Syringe Pumps Table of Contents 7 3 Get Status String G Command 0 0 cece 7 21 7 4 Get Status String GG Command 0 cece tenes 7 21 7 5 Get All Status String G amp Command 0 cee eens 7 22 7 6 Get all status strings from four pump operation G amp 2 Command 7 23 7 7 Gradient Download Commands Single pump flow gradient 7 24 7 8 Gradient Download Commands Two pump flow gradient 7 25 7 9 Gradient Upload Commands Single pump flow gradient 7 26 7 10 Gradient Upload Commands Two pump flow gradient 7 27 7 11 Upload Commands Single pump pressure programming 7 28 7 12 Range Serial Commands 0 0 eee revner 7 28 8 1 Accessing the diagnostic menu 0 cece eee teens 8 4 8 2 Analog Input diagnostic screen 0 cece cette ees 8 4 8 3 Optical sensor interrupted by flag bottom sensor shown rear tower cover plate re MOVER e eere be use pate gas Meany eR M te AE s 8 5 8 4 Serial connection to a computer oo corro 8 6 8 5 Controller case top screws 2 of 4 shown 0 eee nee 8 8 8 6 Pum
117. mum length is 256 with 256 being represented by a 00 This field is eliminated if there are no messages Message field is the area where the actual information is located The maximum length is 256 characters long Checksum is also a 2 digit hexadecimal number This number when added to all the previous characters in the message excluding control characters will result in a sum If there are no errors the result of modulo 256 division of this sum should be 0 Examples Hexadecimal Format Using MODULO Decimal Format Using MODULO Hexadecimal Format Using NO MODULO HL Series Syringe Pumps Section 7 Serial Interface Frame is R304STOPD1 CR R 3 0 4 S TD 0 P 52H 33H 30H 34H 53H 54H 4FH 50H D1H 300H 300H MODULO 256 00 It is important to note that all characters are converted to the ASCII equivalent and added except for the checksum The two characters ofthe checksum are converted to hexadecimal numbers and concatenated to form a single two digit number This number is then converted to its ASCII equivalent and added to the end of the message Step 1 22FH 52H 33H 30H 34H 53H 54H 4FH 50H Step 2 2FH 22FH 100H 2R2FRH Eee 100H 22FH 200H 2FH Step 3 DiH 100H 2FH Step 4 Convert D1H to ASCII Hex and put at end of message Step 5 Put a CR 0DH at the end of message for end of frame Step 1 559 82 51 48 52 83 84 79 80
118. n of 0 1 with a maximum of 9 999 minutes per step If you want to add another step to the file program press INSERT C The step number will increase by one and the default ini tial value will be the final value entered for the previous step Edit as desired When programming is complete press the STORE key to save the file and return to the home screen To start the program press RUN two times M Note When a gradient run is started digital output 8 of the controller ACCESSORY connector will toggle from high to low open to closed for one second Note that an entire gradient program can be removed only by deleting each of its steps one at a time as discussed in Section 4 5 Review Revise amp Hold Options When the last remaining step is deleted the entire file is removed HL Series Syringe Pumps Section 4 Gradient Pumping for Pressure Flow and Concentration Modes 4 4 Dual Pump 4 8 Concentration Gradient Programming Two pump concentration gradients enable proportionate use of two different fluids that combine at the mixer refer to Figures 4 1 and 4 2 This type of pumping uses flow mode FG only single pump gradient program is based on either pressure or flow and con trolled by either time duration in minutes or rate of change units per minute The controller s memory can contain a total of up to 200 steps One program can contain from 1 to 200 steps When operating in grad
119. nce with your company s chemical hygiene plan in the event of a spill In all cases when using Teledyne Isco instrumentation prudence and common sense must be used gt WARNING Team Lift To reduce the risk of injury do not attempt to lift this instrument independently It is highly recommenced that a team lift be used when lifting this instrument I vii HL Series Syringe Pumps Warnings Cautions and Notices viii D AVIS viter de r pandre Les liquides qui sont pomp s dans cet instrument peuvent tre canc rigenes hasards biologiques inflammables ou radioactifs Si vous devez utiliser ces liquides hasardeux il est tr s recommand que vous le faites l int rieur d un environnement isol concu pour tels liquides Cet environnement isol devrait tre construit selon les reglements f d raux provinciaux et locaux aussi que le plan de votre organisation qui concerne l v nement d un accident avec les mati res hasardeuses En tout cas utilisez toujours l instrumentation d Isco avec prudence et sens commun ATTENTION Risque de pincement Ce symbole vous avertit que les mains ou les doigts recevront une blessure s rieuse si vous les mettez entre les l ments en mouvement du m canisme pr s de ce symbole ATTENTION viter les usages hasardeux Si vous utilisez cet instrument d une maniere autre que celles qui sont specifi es dans ce manuel la protection fournie par l instrument peut
120. nd essel 7 20 7 8 2 Gradient Download Command esee 7 23 7 8 3 Gradient Upload Commands 00 ccc rear elles 7 24 7 8 4 Range Command esses 1 28 7 8 5 Error Messages 0 0 e ccc eee eee hh ras 7 29 Section 8 Pump Maintenance Troubleshooting and Servicing 8 1 Introduction ass 0222 st ba Pew oD Ka aaa Se eee ee 8 2 8 1 1 Service Department 0 cee teen eens 8 2 8 1 2 How to Ship Returns 0 ccc es 8 2 8 2 General Cleaning ii ias karpe QE 8 3 8 3 Resetting the System itere p Anen ccc eee e ene 8 3 8 3 1 Basic Reset whee ack e aeg ha yah ne 8 3 8 3 2 Hard Reset i cca pind a de RU ae Pe eee pe nee 8 3 8 4 Diagnostic Ment ov aea ee er seach atia er OL nn det ea 8 4 SAL LCD TEST o AN IR EE 8 4 8 42 ANALOG INPUTS eera raie a ehh 8 4 8 4 3 KEYPAD TEST in ges ate a PE ne EP Eu 8 5 8 44 MOTOR CONTROL LIMITS sssese s 8 5 xiii HL Series Syringe Pumps Table of Contents xiv 84 5 SERIAL TEST 2 Sh cane e ER by ERA REA a ee 8 6 8 5 Removing the Case Top 2 0 cece er 8 7 8 5 1 Controller Case Top Removal 0 0 ccc cece eens 8 7 8 5 2 Pump Case Top Removal 0 00 ccc teen eens 8 8 8 6 b brication eroe ee ele kaka STU ee en 8 8 8 6 1 Ball Nut v s 2 dp SA Re tne ie mut bes sahen ota dened ete 8 9 8 7 Flushing the Cylinder 0 0 ccc cee tence tenes 8 10 8 7 1 Gas Solvent
121. ne voltage 10 second pulses any phase angle random or repetitive DIMENSIONS PUMP CONTROLLER Width 27 18 cm 27 18 cm Depth 46 74 cm 30 48 cm Height 101 09 cm 13 59 cm WEIGHT PUMP CONTROLLER 32 8 kg 2 96 kg FLOW RATE RANGE 1 0 ul min to 107 ml min at any pressure from 0 to 517 1 bar FLOW RATE ACCURACY FLOW RATE DISPLAY RESOLUTION ANALOG OUTPUT ACCURACY 0 5 maximum 0 50 ul min seal leakage 1 0 ul min x 196 of selected range DISPLACEMENT RESOLUTION 6 65 nl REFILL TIME 2 5 minutes REFILL OR DEPRESSURIZATION RATE PRESSURE RANGE 1 0 ul min to 107 ml min for any pressure up to 276 1 bar For higher pressures see Figure 1 2 0 6895 to 517 1 bar PRESSURE ACCURACY 0 5 of full scale at constant temperature PRESSURE REPEATABILITY 0 5 of full scale within 48 hours at constant temperature ZERO PRESSURE DRIFT 0 25 of full scale within 48 hours at constant temperature PRESSURE DISPLAY RESOLUTION AMBIENT TEMPERATURE RANGE TEMPERATURE DRIFT 6 895 kPa 5 to 40 C x 0 15 of full scale C HUMIDITY 9596 maximum CYLINDER CAPACITY 266 05 ml DEAD HEADSPACE VOLUME 2 10 0 020 ml POLLUTION DEGREE INSTALLATION CATEGORY MAXIMUM ALTITUDE a Using water at 137 9 bar and a temperature controlled environment at 30 C b The analog output is an optional accessory c Pressure repeatability specification is based up
122. nee n nes 3 8 3 7 Menu EQU ii A Geta ge bass ad seg aad e Rer UY RR ARR en AS 3 9 3 7 1 Modbus Options 0 0c cee hrs 3 9 3 8 Front Panel Keys ol LRL DL UR WR ERR EH Ge RS ee ses IE 3 9 3 8 1 CONST PRESS and CONST FLOW 00 ccc ene eee 3 9 3 82 PRGM GRAD 000 rete ec Se o E DA 3 9 3 8 3 HOLD oe he cath ec elem ie Ete dii eR iie ede bah 3 9 3184 RECAE Ez 22 erre aet ER E e PDC 3 9 3 8 5 STORE eR Ed teo pes aU Re atia Box CCS RSA 3 9 3 8 6 REFIL 2000 es esses as de ee EANDEM 3 9 3 8 7 LIMITS 000 is re Eee 3 9 3 8 8 RAPID PRESS 4 by 5 305 op ee FRR Aba he ER sed CS Gabe Ras ih 3 11 9 8 9 DISB S v ee EU ee a LX SURVIE RA 3 12 9 840 ACC C TRE ven eek fos Se Oen SEN DH INDE AUR UR ERS OR 3 12 9 8 41 ZERO PRESS 4 4 99e ERG UG CARVER VERE ERR ER 3 12 3 9 Control of Multiple Pumps avuvavvvvrrvr varer rar e 3 13 3 9 1 Multi Pump Operation ssseeeeeee ee 3 13 3 9 2 Independent Control of up to Four Separate Pumps 3 13 3 10 Operating Modes slice EE Recte e a OR en I ede far bd 3 14 9 10 T Constant Flow Acco diae ea a aa na 3 15 3 10 2 Constant Pressure 0 a cc eee 3 15 3 10 3 Dispense Mode ccc een een enn nns 3 16 3 11 External Control ices oe ri Rr fedres ala oe eee e 3 17 3 12 External Control Analog 0 0 ren 3 17 3 12 1 Wire connections ccc cece hh hrs 3 17 3 12 2 Preparation dee nate RE e CE EX PEU 3 17 3 12 3 External
123. ngle controller to operate multiple pumps inde pendently you need to select the appropriate pump run screen before selecting a mode To select the appropriate pump press SELECT PUMP D and an intermediate screen will be shown Press the softkey for the appropriate pump the run screen for that pump will appear Constant Flow Refer to Section 3 10 1 This mode is used when the flow rate must remain constant during the pumping operation Constant Pressure Refer to Section 3 10 2 The constant pressure mode is used when the application of fixed pressure throughout the pumping operation is required The pump will maintain the desired pressure by positive or negative displacement of the piston Programmed Gradient Refer to Section 4 3 10 1 Constant Flow 3 10 2 Constant Pressure HL Series Syringe Pumps Section 3 Basic Programming and Operation In the programmed gradient mode the pump can provide the fol lowing types of gradient Two pump concentration gradient on pumps A and B e Single pump linear pressure gradients on pump A e Single pump flow programs on pump A Dispense For applications requiring delivery of a specific volume Refer to Section 3 10 3 Refill Refer to Section 3 4 3 You can set the refill rate and change it when in refill mode To set constant flow operation use the following procedure 1 Press CONST FLOW CFa will be displayed in the upper left corner of the screen This denotes that yo
124. ode 1 Pump A amp Pump B in Independent mode 1 Pump A amp Pump B in Continuous Flow Constant Flow mode oj ooj gt gt olo l loa gt 1 Pump A amp Pump B in Continuous Flow Constant Pressure mode 1 Pump A amp Pump B in Modifier addition mode 1 Pump A amp Pump B amp Pump C in Continuous Modifier addition mode 1 Pump C amp Pump D in Independent mode 1 Pump C amp Pump D in Continuous Flow Constant Flow mode 1 Pump C amp Pump D in Continuous Flow Constant Pressure mode 0 Multi pump pair AB in Delivery mode 1 Multi pump pair AB in Receive mode 6 4 HL Series Syringe Pumps Section 6 Modbus Configuration Table 6 5 Coils Continued Register Address Type Description 0 Multi pump pair AB in Low Press operation 1 Multi pump pair AB in Normal Press operation 0 Use Fast coarser pressure match control for multi pump pair AB 1 Use Normal finer pressure match control for multi pump pair AB 0 Multi pump pair CD in Delivery mode 1 Multi pump pair CD in Receive mode 0 Multi pump pair CD in Low Press operation 1 Put multi pump pair CD in Normal Press opera tion 0 Use Fast coarser pressure match control for multi pump pair CD 1 Use Normal finer pressure match control for multi pump pair CD 1 Activates the automatic rapid pressurization cycle Constant Flow mode only for Pump A 1 Activates the
125. ode Continuous flow can be used in either of two modes continuous constant pressure or continuous constant flow Both of these modes assume positive displacement of the piston with the exception of continuous Receive Mode for detailed information about Receive Mode refer to technical bulletin TB02 Constant Pressure Pump Operation for Receive Mode available on the Teledyne Isco web site In any syringe pump continuous flow system a flow irregularity occurs at the time of switch over from one pump module to the other This flow irregularity can be measured as a pressure fluc tuation Teledyne Isco pressure fluctuation at switchover is 0 35 bar at system backpressures from 6 9 bar to the single pump maximum Before programming continuous flow appropriate valves must be connected the pumps must contain fluid and there must be backpressure for operation Air valves generally open and close faster and are less prone to error and component wear making them suitable for industrial use and other applications where the system will be constantly running however they require a user supplied pressurized air source of 80 to 115 psi 5 5 to 7 9 bar For 1000 HL Systems 60 1247 143 For 500HL Systems 60 1247 142 For 100HL and 260HL Systems 60 1247 141 5 1 HL Series Syringe Pumps Section 5 Continuous Flow Introduction Installation amp Operation 5 2 Table 5 1 Continuous Flow Technical Specifications
126. olvent changeover 8 10 Specifications 1 2 1 5 System preliminary checkout 1 14 T Technical specifications 1 2 1 5 Total volume reset 3 6 Tubing cutting by hand 2 2 pre cut 2 2 U Unpacking 1 13 v Valve designating as passive active or electric 3 7 user supplied 5 6 Pam HEG amp PIN KIT RI AGIS ER Name and amount of Hazardous Substances or Elements in the product BREA EM B JR BIER Hazardous Substances or Elements Component Name E WK E TT ERIKA ZARA Pb Hg Cd Cr VD PBB PBDE ZR ERIN Circuit Boards 2 a 2 9 o Bee ETS x O O O O O LCD Display ZEHA EA o O O O X O Capacitor Hb Be x O O O O X O Wiring PS FR Zl Internal Cables 9 B 9 S Q JN ERA External Cables 2 2 9 o 9 FH Line Cord ES s 9 i 2 9 AEE BIG X O O O X O Transformer AUR Front Panel Label 9 2 9 9 x 9 NE O X O Keypad a s 9 Y EL DC Motor 5 9 9 p 9 peius SEU KITA AN GE Name and amount of Hazardous Substances or Elements in the product O AURA SDE A DMT AS BEST SERERE ERE KL T gt O Represent the concentration of the hazardous substance in this component s any homogeneous pieces Is lower than the ST standard limitation X RTIA ED E VENA BARA BRST F LE BR ERROR gt LETE EAA gt PRE
127. on To dispense the batch press RUN The sequence runs once then stops To repeat the sequence press RUN again M Note When the system is in Dispense Mode all gradient modes constant pressure mode and auto refill as well as serial con trol are unavailable until Dispense Mode is turned off The pump can be externally controlled for pressure or flow rate operation with an analog voltage or through the serial interface The serial interface allows you to control the pump operation from an IBM PC or compatible computer that has an RS 232 C serial output The serial interface accepts English command words from the computer like constant pressure refill etc For more information see Section 7 Serial Interface The syringe pump can be controlled externally by analog voltage in either constant flow or constant pressure mode The input range is 0 11 5 volts for all pumps with a resolution of 5000 increments per volt WARNING Connecting devices to energized circuits may cause personal injury or property damage Power must be removed from the pump before connecting external devices Two wires are required for analog control The analog common or ground wire should be connected to the GND terminal under ANALOG INPUT of the ACCESSORY connector on the con troller rear panel The analog control or input wire should be con nected to terminal 1 under ANALOG INPUT If two pumps are used with the controller the second analo
128. on Status Pump A S Pump stopped R Running F Refilling H Hold clock stopped E Equilibrating Volume Pump A Units Liters E9 Resolution 1 nanoliter Flow Rate Pump A Units Liters minute E10 Resolution 0 1 nanoliter minute Problem Status Pump A No problem E Empty cylinder B Cylinder at bottom O Over pressure U Under pressure M Motor failure N Pump transduce disconnected Checksum Volume total Continuous flow or modifier mode Units Liters E6 System pressure multi pump displays Pump A for other modes Units psi 5 Resolution 0 2 psi System flow rate multi pump displays Pump A for other modes Units Liters minute E10 Resolution 0 1 nanoliter minute Figure 7 5 Get All Status String G amp Command 7 22 HL Series Syringe Pumps Section 7 Serial Interface Flow Rate Pump A Units Liters minute Pressure Pump A Pressure Pump B Pressure Pump C Pressure Pump D Units psi 5 Resolution 0 2psi 3 ALOG 1 ALOG6 refer to Table 8 5 Units volts 5000 Resolution 0 2 millivolt Range 7500 to 58035 1 5000v to 11 6070v E10 Volume Pump A Units Liters E9 Resolution 1nanoliter Operation Status Pump A S Pump Stopped R Running F Refilling H Holding E Equilibrating Control Status Pump A Resolution 0 1nanoliter minute L Local Control R Remote C
129. on re zeroing pressure transducer every 48 hours Refer to sub section ZERO PRESS in Section 3 of the manual for re zeroing procedure d Volume in and above the piston seal head clearance at automatic shutoff and inlet and outlet ports to the fittings 1 3 HL Series Syringe Pumps Section 1 Introduction Table 1 3 500HL Technical Specifications POWER REQUIREMENTS see Section 1 5 117 12 Vac 3 2 A maximum 234 23 Vac 1 6 A maximum Jractory Set LINE FREQUENCY 50 or 60 Hz LINE VOLTAGE NOISE TOLERANCE 1 7 x nominal rms line voltage 10 second pulses any phase angle random or repetitive DIMENSIONS PUMP CONTROLLER Width 27 18 cm 27 18 cm Depth 46 74 cm 30 48 cm Height 102 36 cm 13 59 cm WEIGHT PUMP CONTROLLER 33 25 kg 2 96 kg FLOW RATE RANGE 1 0 ul min to 204 ml min at any pressure from 0 to 258 6 bar FLOW RATE ACCURACY FLOW RATE DISPLAY RESOLUTION ANALOG OUTPUT ACCURACY 0 5 maximum 1 0 ul min seal leakage 1 0 ul min x 196 of selected range DISPLACEMENT RESOLUTION 12 68 nl REFILL TIME 2 5 minutes REFILL OR DEPRESSURIZATION RATE PRESSURE RANGE 1 0 ul min to 204 ml min for any pressure up to 137 8 bar For higher pressures see Figure 1 3 0 6895 to 258 6 bar PRESSURE ACCURACY 0 5 of full scale at constant temperature PRESSURE REPEATABILITY 0 5 of full scale within 48 hours at constant temperature ZERO PRESSURE
130. on the www modbus org website Modbus Application Protocol Vol 1 and Modbus Over Serial Line Vol 1 and Modbus Messaging on TCP IP The Isco HL Series Syringe pump controller supports both the Modbus RTU and Modbus TCP IP protocols The Modbus RTU protocol implementation uses a 2 wire RS 485 connection The RS 485 connections are located on the DB25 connector labeled RS 232 RS 485 We recommend connecting the ground reference wire as shown in Figure 6 1 The matching connection is a D Subminiature 25 pin standard plug such as an AMP part 747912 2 from an electronic parts supplier AN WARNING Connecting devices to energized circuits may cause personal injury or property damage Power must be removed from the pump before connecting external devices 6 1 HL Series Syringe Pumps Section 6 Modbus Configuration ACCESSORY DIGITAL DIGITAL ANALOG GROUND OUTPUT INPUT A B C 0 10V 1234 1556 1234 CE rer RS 232 RS 485 Pin on DB25 shell RS 485 2 Wire 18 DATA B 11 DATA A 7 GND Figure 6 1 RS 485 Connection 6 1 2 Modbus TCP IP The modbus TCP IP protocol implementation uses an ethernet connection The ethernet connections are located on the DB25 connector labeled USB Ethernet Pin on DB 25 Casing Ethernet Tr
131. ontrol E External Control Problem Status Pump A No problem E Empty cylinder B Cylinder at bottom FFR RO7F 18000 33036 21000 79500 0 2105 2245 4845 500 27067 4000000000 0010696698 F L 0200000000 0057744222 R L 2500000000 0035681623 R L 1000000000 0016778750 R L Pump B Pump C Pump D 44no0000000 18000 p000000000 2500000000 21000 0000000000 T Multipump Pair CD Check sum Volume total continuous flow or modifer mode multi pump pair AB Units Liters E6 System pressure multi pump pair AB displays Pump for other modes Units psi 5 Resolution 0 2 psi System flowrate multi pump pair AB displays Pump A for other modes Units Liters minute E10 Resolution 0 1 nanoliter minute O Over pressure U Under pressure M Motor failure N Transducer disconnected Figure 7 6 Get all status strings from four pump operation G amp 2 Command 7 23 HL Series Syringe Pumps Section 7 Serial Interface 7 8 2 Gradient Download Command 7 8 3 Gradient Upload Commands Step Number 01 LGDL F xx S O1 FL xxx XXXX I xx xx F S XX XX D XXXX X 7 8 upload command Gradient upload commands must follow the format shown in the figures below Where necessary leading and trailing zeros must be included so that the numerical values are represented properly Also note that the single pump gradient commands include spaces in
132. op in place three screws on each side Lift the cover straight up and off Figure 8 6 Pump case top screws 3 of 6 shown 8 6 Lubrication The pump is a precision engineered instrument that must be lubricated every two years or every 6 000 strokes whichever comes first to ensure proper service life The pump has an easy to access lube wheel that keeps the main gears lubricated during operation See Figure 8 7 For your con venience a lubrication kit containing Never seez and DUOLEC 1608 lubricants is included in your pump accessory package 8 8 HL Series Syringe Pumps Section 8 Pump Maintenance Troubleshooting and Servicing Ball Screw Lube Wheel Worm Gear Figure 8 7 Gear train lubrication and motor drive service Worm Worm Gear 8 6 1 Ball Nut The worm and worm gear are lubricated by a lubrication wheel Apply DUOLEC 16068 directly to the wheel until it is saturated The wheel may also be directly lubricated by trickling oil into the wheel while the pump is running M Note Use only DUOLEC 1608 lubrication on the worm and worm gear Do not substitute The ball screw which drives the ball nut must be kept lubricated with Never seez 1 Remove the case top as detailed in section 8 5 and front cover to gain access to all parts requiring lubrication 2 To lubricate the ball nut run the pump until the ball nut reaches its maximum height 3 Apply two beads of lubricant on opposite sides of th
133. oper ating modes of delivery and one independent mode Continuous flow in constant flow mode Continuous flow in constant pressure mode Independent mode A HL Series continuous flow pumping system in constant flow mode will consist of two syringe pumps and a valve package all regulated by one controller Installation and operating instruc tions for this system are provided in Section 5 In these modes the softkeys toggle between the options described in Table 3 1 Table 3 1 Key functions in the Multi pump Mode Display Option Description NORMAL Uses a finer slower pres sure match control when switching from one pump to the other Uses a coarser faster pressure match control when switching from one pump to the other NORMAL PRESS Uses pressure matching when switching from one pump to the other LOW PRESS Uses no pressure matching when switching from one pump to the other DELIVER Sets the pump into the delivery mode of operation RECEIVE Sets the pump into the receive mode of operation MIN MAX POINTS Sets the fill and refill marks that are used with both con tinuous flow modes Sets the NVC feature On Off A HL Series syringe pump controller can run four syringe pumps independently of each other in either constant pressure constant flow mode dispense mode or any combination of the three To set up this option use the following procedure 1 Press MENU 3 13
134. p case top screws 3 of 6 Shown 0 0 ee ees 8 8 8 7 Gear train lubrication and motor drive service 0c eee eee eee ees 8 9 List of Tables 1 1 100HL Technical Specifications 00 eee eee eee 1 2 1 2 260HL Technical Specifications 0 0 eee ee eee 1 3 1 3 500HL Technical Specifications 0 eee eee eens 1 4 1 4 1000HLf Technical Specifications 0 eee eee teens 1 5 1 5 Pump Controller Front Panel Label 0 0 0c cece eee eee 1 8 1 6 Pump Controller Key Functions 0 cece eee eens 1 9 1 7 Pump Controller Rear Panel Connectors 0 00 eee eee eee eee 1 10 1 8 Pump Rear Panel Connectors lese 1 12 1 9 Pump Front Panel coss cid EE e aaa ne 1 13 2 1 HL Series Syringe Pump Accessory Packages lll eee ee ee ees 2 3 2 2 Manual Refill Kits 2 0 0 emm 2 7 2 3 Manual Outlet Valve Kits spati ssaa cece hh 2 9 2 4 Optional in line Filter Package 68 1247 011 lesen 2 9 2 5 Recommended Circulating Bath Volumes 0 0 cee eee eee eee 2 13 3 1 Key functions in the Multi pump Mode 0 00 cece eens 3 13 4 1 Manual Refill Valve Kits eese 4 1 4 2 Manual Outlet Valve Kits 0 0 cece eee eens 4 2 4 3 Swaring Detail tk el4ueeih ke iue diee pl DEI eR a 4 2 5 1 Continuous Flow Technical Specifications Air Valves oooooooo 5 2 5 2 Accessory Control Digital Outputs
135. pair of pliers and the score line Do not squeeze the tubing too tightly as this will flatten or deform its exterior 3 Bend the tubing back and forth to crack it at the score line 4 It may be necessary to deburr the outer tubing ends with the file Make sure the tubing ends are clean and the inner bore is clear before installing the cut tube M Note It is often impossible to remove a burr that blocks the inner bore The accessory packages for all pumps contain fittings that allow you to attach tubing to the pump Note that the tubing is not included 1 Slide first the nut and then the ferrule over the tubing 2 Push the tubing all the way into the port 3 Hold the tubing in place and tighten the nut Table 2 1 HL Series Syringe Pump Accessory Packages Pump Model Part Number 1000HL 60 1269 010 500HL 60 1269 011 260HL 60 1269 012 100HL 60 1269 013 The drip tray outlet on the pump cylinder provides a means of draining fluid from seal leakage Use the l4 ID flexible tubing included with the accessory package to divert the leakage away from the pump To install the drain tube push one end of the tubing over the end of the drip tray outlet as shown in Figure 2 1 M Note The 1000HL has two drain tube outlets one for the drip tray and one for the splash pan located at the bottom of the pump see Figure 2 2 The 1000HL also includes a wash gland as a 2 3 HL Series Syringe Pumps Sect
136. pheric pressure PISTON PLASTIC A TUBE a PURGE CONNECTOR Pad AJ SLIP PLASTIC TUBE OVER O G CYLIN PURGE CONNECTOR 7 Close up view of nitrogen T purge tube This view applies to pumps A other than the 1000D Figure 2 3 Purge connector installation Ay WARNING Liquids expelled by compressed gasses may cause injury Wear eye protection Certain liquids also may require other personal protective equipment Refer to the applicable Material Safety Data Sheet MSDS for more information 2 4 Fluid Connection The optional accessories discussed in this section are used to Accessories make fluid connections from the pump s to another apparatus When making fluid connections that use ferrules be sure to use the ferrules provided in the kit Push the tubing completely into the connector and finger tighten Then tighten with a wrench to clamp the ferrules onto the tubing 2 4 4 Manual Refill Kit The optional manual refill kit provides a high pressure two way valve that connects to the pump inlet to a fluid reservoir The kit contains all tubing and hardware necessary for valve instal lation Kit components and connections are shown in Figure 2 4 2 6 Kit installation HL Series Syringe Pumps Section 2 Fluid System Connections amp Accessories 1 To attach the two way valve to the pump housing use the valve spacer block and screws pro
137. ple program capable of running one controller and one to three pumps Its primary purpose is to provide an example to help the programmer start constructing custom pro grams for individual pump systems and applications To modify the program in any way you must have the complete LabView Compiler available from LabView s manufacturer National Instruments Corporation www ni com Tel 800 531 5066 Fax 512 683 8411 11500 N Mopac Expwy Austin TX 78759 3504 This section of the manual provides detailed information about Cable Connections for Serial Control Setting up the Controller s for Serial Control DASNET Protocol Serial Commands 7 1 HL Series Syringe Pumps Section 7 Serial Interface 7 2 Network Control and Network communications are always initiated by the network Communication controller which is typically a computer Messages from the instruments are in response to messages from the network con troller All information on the network is transmitted as groups of ASCII characters called frames The message frames contain the origin of the message the destination of the message and a checksum to verify the validity of the message Each instrument is provided with a method of selecting unit identification numbers and a baud rate For proper operation each instrument must be set to a unique unit identification number see Section It is also important that each unit s baud rate is set to the same speed Possible baud
138. res 4 1 and 4 2 on the following page Valve kits for other models include male adapter fittings A gradient pumping system includes high pressure two way valves that connect the pump inlets to fluid reservoirs and the pump outlets to the gradient mixer dual pump system or other apparatus single pump system Each refill kit and outlet valve Two Way package contains one two way valve the gra Valve part dient package contains two The kits contain 209 0098 05 all tubing and hardware necessary for valve installation 4 2 HL Series Syringe Pumps Section 4 Gradient Pumping for Pressure Flow and Concentration Modes Following installation the tubing connections must be tested for leaks before any program is run If a leak is found tighten the connection slightly If the leak persists swage the connection again with a new ferrule Refer to Technical Bulletin TB05 Field Verification Procedures at http www isco com sp_applications techbulletins for leak test procedures 4 3 HL Series Syringe Pumps Section 4 Gradient Pumping for Pressure Flow and Concentration Modes 4 2 1 Inlet Connections Kit components and connections are shown in Figure 4 1 1 Mount the inlet valve on the pump housing with the spacer block and screws provided 2 Connect the pre bent SST tubing from one port of the valve to the pump inlet Use the nut and ferrule to connect the tubing at the inlet and the valve fittings to connect the tub ing
139. rns the pressure integral control On and Off for the pump indicated IPUMPB 1 IPUMPB 0 1 ON IPUMPC 1 IPUMPC 0 0 o OFF IPUMPD 1 IPUMPD 0 u LGE F XX A 0X Action to perform when gradient program reaches the end The pump needs to be in local mode only File F 01 99 Action A 00 Hold final value 01 Stop after final step 02 Return to initial value and hold 03 Return to initial value and repeat program LGSL F xx Start Gradient Command This starts a gradient program same as the RUN key This command will check to see if there is a gradient running and respond with RUNNING if there is The pump needs to be in local mode only Select Gradient File Command This selects a gradient file to be run This command will reset the controller to the saved file gradient type If the selected gradient file does not exist the controller will respond with PROBLEM INVALID OPERAND The pump needs to be in local mode only LGST Stop Gradient Command This stops a gradient program The pump needs to be in local mode only LGDL F xx S xx Gradient Step Download command This downloads a step from the pump to the PC This command will respond with PROBLEM INVALID OPERAND if the file or step does not exist The controller will respond with step information if the command is valid Refer to 7 8 2 for complete information on Gradient step download com mands The pump needs to be in local mode only LGUL F xx
140. s pense mode Volume being dispensed by Pump D in Dis pense mode illiliters 6 10 Register Table 6 6 Holding Registers Continued of Registers HL Series Syringe Pumps Section 6 Modbus Configuration Description Full scale input range voltage for External mode Volts MIN MOD PRESS Minimum modifier pump pressure ATM BAR kPa PSI See NOTE 1 Value of B for Modifier addition mode C for Continuous Modifier addition mode B FOR MODIFIER OR C FOR CONTIN UOUS MODIFIER Absolute maximum pressure for Pump A ATM BAR kPa PSI See NOTE 1 Absolute maximum flow rate for Pump A ml min ml hr ul min ul hr See NOTE 2 Absolute maximum refill rate for Pump A ml min ml hr ul min ul hr See NOTE 2 Maximum volume for Pump A Absolute maximum pressure for Pump B milliliters ATM BAR kPa PSI See NOTE 1 Absolute maximum flow rate for Pump B Absolute maximum refill rate for Pump B ml min ml hr ul min ul hr See NOTE 2 ml min ml hr ul min ul hr See NOTE 2 Maximum volume for Pump B Absolute maximum pressure for Pump C milliliters ATM BAR kPa PSI See NOTE 1 Absolute maximum flow rate for Pump C Absolute maximum refill rate for Pump C ml min ml hr ul min ul hr See NOTE 2 ml min ml hr ul min ul hr See NOTE 2 Maximum volume for Pump C Absolute maximum pre
141. s the inlet or outlet You may plug one port and use a single port as both the inlet and outlet e 1000HL 4 NPT e 500HL le NPT 100HLg 260HL 18 Valco M Note Custom caps are available with different port configurations and sizing Contact the factory for details HL Series Syringe Pumps Section 2 Fluid System Connections amp Accessories 2 3 Fluids Certified for Isco HL Pumps 2 3 1 Installation Tips 2 3 2 Tubing Requirements Purchased cut tubing Cutting the tubing 2 2 AN WARNING Pumping fluids that are not compatible with the HL Series Pump may damage critical components resulting in property damage personal injury or even death Users who pump fluids other than those listed below do so at their own risk The fluid contacting materials within the HL Series Syringe Pump have been tested and certified to be compatible with the following fluids ethane ethylene propane propylene butane butadiene hexane gasoline diesel fuel e jet fuel ammonia methanol e isopropanol acetone e acetonitrile heptane benzene e ethylbenzene methane Consult with Isco before using any chemical not listed above Be sure to keep the tubing as straight as possible at the end as this will make it easier to install the ferrules Be sure to cut the ends of the tubing squarely Do not leave burrs on the ends of the tubing When installing ferrules on the tubing be sure the tubing
142. se Mode C Only configure if in Constant Flow Mode 3 3 3 3 5 5 5 5 5 SS 5 5 Sp 5 0 Disable Dispense Mode D 1 Enable Dispense Mode D Only configure if in Constant Flow Mode 1 Reset total volume delivered by multi pump pair AB 1 Reset total volume delivered by multi pump pair CD Pressure Units ATM Pressure Units BAR Pressure Units kPa Pressure Units PSI Flow Units ml min Flow Units ml hr Flow Units ul min Flow Units ul hr 0 Overpressure Alarm OFF 1 Overpressure Alarm ON 0 Overpressure Display OFF 1 Overpressure Display ON 0 Overpressure Shutdown OFF 1 Overpressure Shutdown ON 0 Underpressure Alarm OFF 1 Underpressure Alarm ON 0 Underpressure Display OFF 1 Underpressure Display ON 0 Underpressure Shutdown OFF 1 Underpressure Shutdown ON 0 Overflow Alarm OFF 1 Overflow Alarm ON 0 Overflow Display OFF 1 Overflow Display ON 0 Overflow Shutdown OFF 1 Overflow Display ON 6 6 HL Series Syringe Pumps Section 6 Modbus Configuration Table 6 5 Coils Continued Register Address Type Description 0 Underflow Alarm OFF 1 Underflow Alarm ON 0 Underflow Display OFF 1 Underflow Display ON 0 Underflow Shutdown OFF 1 Underflow Shutdown ON 0 Poor Fill Alarm A OFF 1 Poor Fill Alarm A ON 0 Poor Fill Alarm B OFF 1 Poor Fill
143. serial control refer to Section 7 in this manual This option displays the controller software revision and model of connected pump s The first line displays the revision of the software Lines 2 3 and 4 display the type of pump connected to the A B C and D pump connectors respectively This screen is also momentarily displayed automatically each time the pump controller is switched on The pump pressure or flow rate operation can be controlled externally with an analog voltage Complete instructions for this feature are provided in Sections 3 12 through 3 12 5 One controller can control up to four pumps at once either together or independent of each other Complete information about using the MULTI PUMP feature is pro vided in Section 3 9 and Section 5 6 This option resets the total volume display of multi pump pair AB or multi pump pair CD to zero when operating in continuous flow or modifier mode 1 AB PUMPS 2 CDPUMPS 3 AB AND CD PUMPS 3 5 6 Valve 3 6 Menu Three HL Series Syringe Pumps Section 3 Basic Programming and Operation This feature identifies the type of valves passive or active air being used for the flow operation Valves are set to active by default If active value is selected the controller will match the pressure more closely before switching delivery pumps The number for the selected valve type will be blinking Press 1 or 2 to select the correct valve type
144. sion Hazard Substitution of components may impair suitability for use in Class I Division 2 environments Ay WARNING Connecting devices to energized circuits may cause personal injury or property damage Power must be removed from the pump before connecting external devices Ay WARNING Explosion Hazard The area must be known to be non hazardous before servicing replacing the unit and before installing or removing I O wiring Do not disconnect equipment unless power had been disconnected and the area is known to be non hazardous 1 HL Series Syringe Pumps Section 1 Introduction Table 1 1 100HL Technical Specifications POWER REQUIREMENTS see section 1 5 117 12 Vac 3 2 A maximum 234 23 Vac 1 6 A maximum Jractory Set LINE FREQUENCY 50 or 60 Hz LINE VOLTAGE NOISE TOLERANCE 1 7 x nominal rms line voltage 10 second pulses any phase angle random or repetitive DIMENSIONS PUMP CONTROLLER Width 27 18 cm 27 18 cm Depth 46 74 cm 30 48 cm Height 101 09 cm 13 59 cm WEIGHT PUMP CONTROLLER 32 8 kg 2 96 kg FLOW RATE RANGE 0 01 ul min to 60 ml min at any pressure from O to 689 5 bar FLOW RATE ACCURACY 0 3 maximum 0 25 ul min seal leakage FLOW RATE DISPLAY RESOLUTION 0 01 ul min 1 0 ul min in Constant Pressure Mode ANALOG OUTPUT ACCURACY DISPLACEMENT RESOLUTION REFILL TIME x 196 of selected range 1 72 minutes REFILL OR DEPRESSURIZA
145. sponds to commands accordingly The HL Series pump functions as a slave unit These functions may be combined in one unit i e a computer can function as both a network controller and a master All data transfers are in a frame format When the network con troller polls an instrument it will start to respond within 200 ms If it does not reply it will be polled again If after three attempts at polling it does not reply it will be dropped from the polling rotation When the instrument does respond the polling rotation does not advance until an error free transfer has occurred The frame format for data transfers from the network controller is as follows destination acknowledgement message source length message checksum CR The destination is the 1 digit unit identification number of the instrument to receive the message Acknowledgment is one character to indicate the success of the previous transmission There are three possibilities 1 E means error resend the message immediately E is sent by the network controller only Other units signify errors by not replying causing the controller to resend the message 2 B means busy resend message at next poll 3 R signifies previous message was received Message source is the unit ID of the unit that origi nated the message If there is no message this location is a space 20H Length is the length of the message in 2 digit hexadecimal format Maxi
146. ss HOLD or RUN The RECALL key can only be used when the pump s are stopped or when in HOLD mode The RECALL key is used to recall a pre viously programmed gradient When you select this option the controller automatically loads the gradient and switches the pump to program gradient mode 1 Press RECALL and use the number keys to enter the num ber name of the gradient you wish to recall Press ENTER 2 If you enter a number of a gradient that does not exist the controller briefly displays the message FILE NUMBER DOES NOT EXIST It then assumes you will be creating a new gradient under that number and displays the program gradient run screen The STORE key is operational in the programmed gradient mode It is used to save the program gradient parameters and exit the programming parameter entry mode The REFILL key manually places the pump in refill mode The controller allows the user to set the minimum and maximum flow rate limits the minimum and maximum pressure limits and the maximum rate the pump will run while controlling the pressure in constant pressure mode e When using a single controller to operate multiple pumps you need to select the appropriate pump before setting any pump parameters The available pumps will be displayed above the softkeys These selections corre 3 9 HL Series Syringe Pumps Section 3 Basic Programming and Operation spond with the connector that the pump control cable is plugged
147. ssure 1 Pump D is underpressure 1 Motor failure Pump D 1 POOR FILL A ERROR 1 POOR FILL B ERROR 1 POOR FILL C ERROR 1 POOR FILL D ERROR 1 VALVE ERROR DIGITAL IN 1 DIGITAL IN 2 DIGITAL IN 3 3 D D D D D D D D D D D D D D D D D D D D D D D D D D D D D O 6 7 HL Series Syringe Pumps Section 6 Modbus Configuration Table 6 5 Coils Continued Register Address Type Size Description Table 6 6 Holding Registers Register Address Type of Registers Description Pressure set point for Pump A in Constant Pressure mode kPa PSI See NOTE 1 Pressure set point for Pump B in Constant Pressure mode kPa PSI See NOTE 1 Pressure set point for Pump C in Constant Pressure mode Pressure set point for Pump D in Constant Pressure mode kPa PSI See NOTE 1 kPa PSI See NOTE 1 Pressure set point for multi pump pair AB in Continuous Flow Constant Pressure mode kPa PSI See NOTE 1 Pressure set point for multi pump pair CD in Continuous Flow Constant Pressure mode ow rate set point for Pump A in Constant ow mode kPa PSI See NOTE 1 ml min ml hr ul min ul hr See NOTE 2 ow rate set point for Pump B in Constant ow mode ml min ml hr ul min ul hr See NOTE 2 ow rate set point for Pump C in Constant ow mode ow rate set point for Pump D in
148. ssure for Pump D milliliters ATM BAR kPa PSI See NOTE 1 Absolute maximum flow rate for Pump D Absolute maximum refill rate for Pump D ml min ml hr ul min ul hr See NOTE 2 ml min ml hr ul min ul hr See NOTE 2 Maximum volume for Pump D PUMP TYPEA milliliters PUMP TYPE B PUMP TYPE C PUMP TYPE D SOFTWARE MAJOR REV SOFTWARE MINOR REV D D SOFTWARE MICRO REV M Note Pressure and flow rate based on user selected units HL Series Syringe Pumps Section 6 Modbus Configuration 6 12 7 1 Overview HL Series Syringe Pumps Section 7 Serial Interface The Teledyne Isco HL Series pump can be remotely controlled by a computer through a built in RS 232 C serial or USB interface This function is supported by the Teledyne Isco LabView toolkit You can write your own custom program to control Isco syringe pumps using any format such as BASIC or C This section provides the syntax and responses for serial commands Sim plified examples of programs are also provided It is possible for you to write custom programs capable of controlling up to 7 con trollers from a single computer each with up to 4 connected pumps Writing programs for serial control requires substantial knowledge of the software language used so Isco does not provide support for developing your own programs The toolkit is a sam
149. status of the digital output control bits as either REMOTE R or INTER NAL I The return message format is DIG CONTROL xxxxxx where x is either R or I R indicates the corresponding bit is controlled remotely and I indicates the corresponding bit is controlled internally by pump software The status order returned corresponds with the outputs 1 8 DIG CONTROL command 1 8 TR Sets the digital output control bits to either internal or remote where x is either R for REMOTE or I for INTERNAL CONTROL R indicates the corresponding bit will be controlled remotely through the serial port I indicates the corresponding bit will be controlled internally by pump software The order corresponds with the out puts 1 8 DISPENSEA DISPENSEB DISPENSEC DISPENSED Returns the dispense volume for Dispense mode DISPENSEA DISPENSEB DISPENSEC DISPENSED Sets the dispense volume for Dispense mode Format is XXX XXX ml Leading and trailing zeros are not required Can only be changed when pump is stopped FLOW FLOWCD Returns the delivering pump s flow rate in continuous pumping mode and modifier addition mode In INDEPENDENT mode FLOW returns the pump A flow rate and FLOWCD returns the pump C flow rate FLOWA FLOWB FLOWC FLOWD Returns the actual flow rate of the pump FLOW FLOWB FLOWC Returns the actual flow rate of the pump Enter for
150. sub D PRESSURE TRANSDUCER connector on the pump rear panel Figure 1 7 Be sure to tighten the screws 2 Connect the control cable which originates from the pump rear panel to the PUMP A connector on the rear panel of the controller Figure 1 6 and tighten the screws This cable must be plugged into the PUMP A connector There are four PUMP connectors on the rear of the controller Only PUMP A is wired to supply power to the controller there fore one pump must be attached to the PUMP A connector 3 Check the serial number tag to make sure the voltage rat ing of the pump is correct Ay WARNING Do not make or separate connections while circuits are energized AC mains power must be permanently wired to the AC power source Never attach a plug or power cord to the wires Electrical connections must be made according to governing directives 4 Connect the AC Mains Power wiring from PUMP A to an AC power source All input and output circuits must be wired using Division 2 wiring methods as specified in Arti cle 501 4 b of the National Electrical Code NFPA 70 for installations HL Series Syringe Pumps Section 1 Introduction 1 6 Preliminary Checkout Hard reset System Reset System Checks Figure 1 9 Status Screen After the electrical connections have been completed follow this brief test of the pump s operation Perform a hard reset 1 Ensure that power has been removed from the pump con troller 2
151. t to an appro priate length Nut ferrule To refill kit assembly SST tubing gt Screws Reducing union SST tubing to system M 4 5 HL Series Syringe Pumps Section 4 Gradient Pumping for Pressure Flow and Concentration Modes 4 3 Single Pump Gradient A single pump gradient program is based on either pressure or Programming flow and controlled by either time duration in minutes or rate of change units per minute The controller s memory can contain a total of up to 200 steps One program can contain from 1 to 200 steps When operating in gradient mode any connected pumps not used for gradient are inoperable 1 To access the gradient programming menus press PRGM GRAD select PRESSURE 2 or FLOW 3 and CONTINUE C The home screen will appear with either PG Pressure Gradient or FG Flow Gradient in the upper left corner PGa 0 000mL MIN 2PSI 013 28mL STOPPED FILEN 00 00 00 MIN FINAL PSI PROGRAM EDIT REVIEW OPTION 2 Press PROGRAM A 3 At the prompt enter a file number between 1 and 99 This is the file name of your program and can be the name ofa new program you are creating or a stored program you want to edit or run Press ENTER M Note If a selected stored program is in a different mode than that of the controller selected in Step 1 a brief notification will appear displaying the controller s mode and the file s mode
152. tems when changing from one liquid solvent to another If high ionic strength aqueous reagent solutions are allowed to remain in the pump solid residues may be formed which will scratch the seals and the polished inner surface of the cylinder of the pump These scratches allow leakage which decreases flow rate 1 Press CONST FLOW gt RUN You will be asked to designate which pump if more than one is present 2 Press a softkey to run the desired pump 3 Run the pump until the message CYLINDER EMPTY is displayed 4 Place the pump inlet line in a flask containing a compati ble solvent or a detergent solution 5 Press REFILL You will be asked to designate the pump to refill if more than one is present Press a softkey to refill the pump 6 Fill the pump and repeat this procedure several times 7 Place the pump inlet line in a flask containing distilled water or appropriate solvent Fill the pump once more and HL Series Syringe Pumps Section 8 Pump Maintenance Troubleshooting and Servicing then run it until empty You are now ready to fill the pump with the new liquid solvent M Note Do not leave buffer solutions which contain dissolved salts or corrosive buffers in the cylinder overnight or for long periods of time The pump should be stored with methanol or isopropanol at least partially fill the cylinder with either solvent and then run the piston all of the way up when it is not being used HL Series S
153. the command string Gradient Duration Final Gradient Percentage Range 00 00 100 0 Initial Gradient Percentage Range 00 00 100 0 Gradient Total Flow Rate Units milliliters minute Range 000 0000 Maximum Flow Rate L Step Number 01 File 01 99 Step Numbers 02 through 99 LGDL F xx S xx I xx XX F XX XX D XXXX X Gradient Duration Range 0000 1 9999 0 minutes Final Gradient Percentage Range 00 00 100 0 Initial Gradient Percentage Range 00 00 100 0 Step Number 02 99 File 01 99 Figure 7 7 Gradient Download Commands Single pump flow gradient 7 24 This command downloads a step from the HL Series pump to the PC This command will respond with PROBLEM INVALID COMMAND if the file or step does not exist If the file and step is valid the controller will respond as shown in Figures 7 7 and These commands upload a step from the PC to the HL Series pump This command will respond with PROBLEM INVALID COMMAND if the file or step does not exist If the file and step is valid the controller will respond as shown in Figures 7 9 and 7 10 Figure 7 11 shows an example of a pressure programming Range 0000 1 9999 0 minutes HL Series Syringe Pumps Section 7 Serial Interface Step Number 01 LGUL F xx S 01 FL xxx Xxxxx IB xx xx FB xx Xxx D XXXX X
154. the one shown in Figure 7 12 RO2CP 10000 F 2500000 R 30 00000 V 102 9600 40 l l filed Checksum Max Volume 102 96 Units milliliters Max Refill Rate 30 0 milliliters minute Max Flow Rate 25 milliliters minute Maximum Pressure 10 000 psi Figure 7 12 Range Serial Commands 7 28 HL Series Syringe Pumps Section 7 Serial Interface 7 8 5 Error Messages If an error occurs in a message one of the following responses will be sent The format of an error message is PROBLEM Refer to Table 7 6 Table 7 6 Error Messages Error Description PROBLEM LOCAL MODE The pump was sent a command before being placed in remote mode See Section 7 8 PROBLEM INVALID COMMAND The command sent was not recog nized by the pump PROBLEM INVALID OPERAND The operand character s following the sign is missing or is incorrect e g the number was too large PROBLEM PUMP RUNNING The command sent is only valid when the pump is stopped PROBLEM OVERPRESSURE Sent in response to a high or low PROBLEM UNDERPRESSURE pressure limit condition PROBLEM CYLINDER EMPTY Sent when the pump cylinder is empty PROBLEM CYLINDER FULL Sent when the pump cylinder is full PROBLEM NO PUMP Sent when the pump is not present PROBLEM WRONG PUMP MODE Sent when the pump is in the incor rect mode for the command 7 29 HL Series Syringe Pumps Section 7 Serial Int
155. to automatically refill when a certain volume is reached From Menu 2 the system can be programmed to automatically refill a pump based on an external analog signal with a range of 0 to 11 5 volts To access this feature press MENU gt MORE A gt EXTERNAL 3 FULL SCALE INPUT RANGE lt XX XXX VOLTS 1 SET RANGE OFF OFF OFF DIGITAL EXTERNAL REFILL PREVIOUS To set the full scale value maximum 11 5 volts press SET RANGE 1 enter the desired value and press ENTER To place the pump under external control press EXTERNAL B The feature will turn from OFF to ON To enable remote RUN STOP control press DIGITAL A This feature will turn from OFF to ON To set the external control for refill press REFILL C The feature will turn from OFF to ON 3 12 4 Calculation Examples 3 12 5 Setup HL Series Syringe Pumps Section 3 Basic Programming and Operation Flow Rate If MAX FLOW is set at 25 ml min in the LIMITS menu and the desired scale factor is 5 0 volts per 20 ml min do the fol lowing to determine the analog voltage range 35 25x 3 625 In this example the analog voltage range would be 0 0 6 25 volts Pressure If MAX PRESS is set at 510 2 ATM in the LIMITS menu and the desired scale factor is 2 0 volts per 100 ATM do the fol lowing to determine the analog voltage range 2 510 2 x 5 10 204 In this example the analog voltage range would be 0 0 10 204 volts First se
156. tput Coils Write Multiple Analog Output Holding Regis ters Table 6 4 Exception Responses Exception Code Name Description Function code received is 01 Illegal Function not supported 6 3 HL Series Syringe Pumps Section 6 Modbus Configuration Table 6 4 Exception Responses Continued Data address received is legal Address not an allowable address A value contained in the query data field is not an allowable value for the addressed location This may indicate a fault in the structure of the remainder of a complex request such that the implied length is incorrect Does NOT mean data value is outside the expectation of the controller Illegal Data Value Table 6 5 Coils Register Address i Description 00001 0 Stop Pump A 1 Run Pump A 00002 0 Stop Pump B 1 Run Pump B 00003 0 Stop Pump C 1 Run Pump C 0 Stop Pump D 1 Run Pump D Link Run Stop operation of multi pump pair AB amp multi pump pair CD 0 Disable 1 Enable 0 Stop Pump A if in Refill 1 Run Pump A in Refill 0 Stop Pump B if in Refill 1 Run Pump B in Refill 0 Stop Pump C if in Refill 1 Run Pump C in Refill 0 Stop Pump D if in Refill 1 Run Pump D in Refill 9 Pump A in Constant Pressure mode 1BIT 1 Pump C in Constant Pressure mode Pump A in Constant Flow mode wj rm mo Pump B in Constant Flow mode Pump C in Constant Flow mode Pump D in Constant Flow m
157. trl 3 12 help 3 12 hold 3 9 limits 3 9 multi pump mode 3 13 5 8 rapid press 3 11 recall 3 9 store 3 9 Zero press 3 12 L Liquid connections drain tube 2 3 M Main menu 3 3 units 3 3 Maintenance 8 1 flushing the cylinder 8 10 lubrication 8 8 removing the case top 8 7 seal cleaning and replacement 8 10 Index 1 HL Series Syringe Pumps Index Index 2 solvent changeover 8 10 Teledyne Isco Service 8 2 Master network 7 10 Menus display contrast 3 5 main menu 3 3 power failure 3 5 pump status 3 6 refill 3 4 serial options 3 6 total volume reset 3 6 Modes of operation 3 14 Multi pump 3 6 key functions 3 13 5 8 N Network 7 10 Noise reduction 2 10 O Operating modes 3 14 Operational test 1 14 Outlet valve accessory package 2 7 P Power failure actions 3 5 Preliminary checkout 1 14 Pressure drift 3 12 limits setting 3 9 rapid pressurization 3 11 Pump front panel 1 13 rear panel connectors 1 12 status 3 6 R Refill kit 2 6 Refill rate setting 3 4 Remote run stop 3 19 Repairs 8 2 Returning equipment 8 2 S Safety 1 i 1 viii Seals cleaning and replacement 8 10 Serial control cabling 7 15 commands 7 15 error messages 7 29 examples 7 11 set up 7 29 Serial options 3 6 Service Department 8 2 Slave network 7 10 Software continuous flow 5 6 DASNET 7 6 example BASIC 7 8 example C 7 9 user written 7 6 S
158. u will be defining constant flow parameters for pump If you wish to define parameters for pump B C or D press softkey D select pump and then press softkey A B C or D to select pump B C or D respectively M Note If the main menu is displayed you must press D under CONST FLOW 2 Press A to change the flow rate The words ENTER FLOW RATE will flash on the screen 3 Use the number keys to enter the desired flow rate M Note If you make an error press CLEAR ENTRY to delete one char acter at a time 4 Press ENTER once the desired flow rate is displayed 5 Press RUN to begin pump operation Programming a constant pressure operation only requires a few keystrokes Use the following procedure 1 Press CONST PRESS CPa will be displayed in the upper left corner of the screen This denotes that you will be defining constant pressure parameters for pump A If you wish to define parameters for pump B C or D press soft key D select pump and then press softkey A B C or D to select pump A B C or D respectively HL Series Syringe Pumps Section 3 Basic Programming and Operation 3 10 3 Dispense Mode M Note If the main menu is displayed you must press softkey D under RETURN before pressing CONST PRESS 2 Press the A softkey to indicate to the program that you wish to enter the pressure The words ENTER PRES SURE will flash on the screen 3 Use the number keys to enter the desired pressure
159. ugh ACCESS CTRL When air valves are used lights on the air valve actuator indicate which valves are open Table 5 3 Key functions in the Multi pump Mode Key Display Option NORMAL Description Uses a finer slower pres sure match control when switching from one pump to the other Uses a coarser faster pressure match control when switching from one pump to the other NORMAL PRESS Uses pressure matching when switching from one pump to the other LOW PRESS Uses no pressure matching when switching from one pump to the other DELIVER Sets the pump into the delivery mode of operation RECEIVE Sets the pump into the receive mode of operation OTHER OPTIONS Sets the fill and refill marks that are used with both con tinuous flow modes Sets the NVC feature On Off Select Pump This menu allows you to select any pump to display its run screen program and operation data and to make program changes Valve specification To prevent pressure fluctuation at swi tchover you must specify the type of valve package you are using HL Series Syringe Pumps Section 5 Continuous Flow Introduction Installation amp Operation gt V AIR CHECK VALVES MORE 2 WAY 3 way ELECTRIC ELECTRIC PREVIOUS Press 3x to return VALVE to run screen Figure 5 4 Keystrokes to specify valve type M Note In Figure 5 4 Active refers to air or electric valves Passive re
160. user settings This is a basic reset For information about performing a hard reset refer to Section 8 3 2 From the menu you can adjust the screen brightness for your light conditions and viewing angle Use softkeys B DOWN or C UP to reduce or increase the brightness Menu 2 provides programming options for serial communication pump status external control multiple pump operation volume reset and valve control To save all settings and return to the main menu press PRE VIOUS RETURN D 1 SERIAL OPTION 4 MULTI PUMP 2 STATUS 5 TOTAL VOL RESET 3 EXTERNAL 6 VALVE MORE PREVIOUS Figure 3 3 Menu 2 program selections 3 5 HL Series Syringe Pumps Section 3 Basic Programming and Operation 3 5 1 Serial Option Figure 3 4 Serial option menu 3 5 2 Pump Status 3 5 3 External Control 3 5 4 Multiple Pumps 3 5 5 Total Volume Reset Figure 3 5 Total volume reset 3 6 The serial option menu allows you to set the baud rate and the unit identification number BAUD RATE 1200 UNIT ID 6 NEXT BAUD NEXT ID PREVIOUS A B C D Use softkey A NEXT BAUD to scroll through the available baud rates These are 300 1200 2400 4800 9600 19 2K 38 4K 57 6K and 115 2K Use softkey B NEXT ID to scroll through the available ID numbers These are 1 7 Six is the default as this is the unit identifier for the Teledyne Isco LabView software For a complete discussion of
161. utely necessary that you understand everything in English above all technical terms before operating the instrument Otherwise it is necessary for you to receive complete instruction from someone qualified who understands both the instrument and English very well Um eine Besch digung des Ger tes oder eine Gef hrdung des Anwenders zu vermeiden ist es notwendig daB dieser vollstandig die englische Sprache und die technischen Bezeichnungen beherrscht Oder der Anwender muB von einer Person einge bt werden die bereits vorher dieses Ger t bedienst hat Pour emp cher dommage l instrument ou blesser vous m me il faut absolument que vous compreniez tout en anglais surtout les termes techniques avant d actionner l instrument Autrement il faut que vous receviez l instruction parfaite d une personne tr s comp tente qui comprend bien les deux l instrument et anglais Para prevenir cualquier dafio en el instrumento o en el operador es necesario que el usuario comprenda perfectamente el lenguaje ingl s y las t rminos t cnicos intr nsecos o bien ser formado por una persona que haya trabajado ya previamente con este instrumento For a forhindre skade p instrumentet eller operat ren er det n dvendig at brukeren har full forst else for det engelske sprak og tekniske uttrykk Ellers m brukeren f oppl ring av en person som kan engelsk for instrumentet tas i bruk F r att f rhindra skade p
162. vided a For the 500HL pump screw the male adapter into the inlet port of the pump Connect the pre bent stainless steel tubing from the valve to the pump inlet Use the nut and ferrule to connect the tubing at the inlet and the valve fittings to connect the tub ing at the valve Connect the PTFE refill tubing with the filter to the port of the two way valve using the nuts and ferrules supplied I Note id When connecting to pressurized sources in super critical fluid applications use the stainless steel tubing without a filter An in line filter is contained in the CO connection package refer to Section 2 4 3 Nut ferrule To system Spacer block Pre bent SST tubing Valve PTFE m tubing or AL stainless steel tubing lt Filter Figure 2 4 Refill kit installation Table 2 2 Manual Refill Kits Pump Model Part Number 1000HL 68 1247 117 500HL 68 1247 083 100HL 260HL 68 1247 077 NDANGER RISK OF INJURY THIS EQUIPMENT PRODUCES HAZARDOUS PRESSURES PLEASE UTILIZE APPROPRIATE TUBING AND CONNECTIONS AS NOTED IN THE MANUAL 2 4 2 Manual Outlet Valve The optional manual outlet valve kit provides manual control of Kit the pump outlet port by connecting a shutoff valve between the pump and the rest of the system Kit installation 1 Attach the two way valve using the spacer block and pan head screws HL Series Syringe Pumps Section 2 F
163. xerted on the secondary wash gland seal During operation the wash gland outlet should always be uncapped and routed for either recirculation or drainage HL Series Syringe Pumps Section 2 Fluid System Connections amp Accessories Detailed information about pumping salt solutions and brines is available in technical bulletin TB04 Pumping Salt Solutions and Brines Nitrogen purge port 1000D only Drip tray outlet Wash gland tubes Splash pan outlet Pump Outlet Fluid User supplied Figure 2 2 Drain tube installation and wash gland connection M Note Wash gland options are available for the 500HL syringe pump with 16 wash tubes Contact Teledyne Isco for more informa tion 2 3 6 Cylinder Washing All pumps that do not have a wash gland are equipped with a Nitrogen Purge purge connector tube near the top of the pump body The purge connector enables the pump cylinder beneath the piston to be purged with nitrogen which can increase useful cylinder life by flushing residue from the system Figure 2 3 shows a typical con nection to the purge tube on the back of the pump 2 5 HL Series Syringe Pumps Section 2 Fluid System Connections amp Accessories To purge with nitrogen 1 Attach gas supply by slipping the plastic tube over the purge connector as shown below 2 Regulate the nitrogen supply to slightly above atmos
164. yringe Pumps Section 8 Pump Maintenance Troubleshooting and Servicing 8 12 HL Series Syringe Pumps Index HL Series Syringe Pumps Index A Acc ctrl key 3 12 Accessories back pressure regulation 2 13 CO cylinder connection package 2 7 cooling heating jacket 2 11 cylinder insulating cover 2 10 in line filter package 2 9 manual control 3 12 outlet valve package 2 7 refill kit 2 6 Air valve package 5 1 Alarms pressure limits 3 11 Automatic refill 3 4 B Back pressure regulation 2 13 Baud rate 3 6 c Check valve package 5 1 CO cylinder connection package 2 7 Commands serial 7 15 Constant flow mode 3 15 Constant pressure mode 3 15 Contact Service Department 8 2 Continuous flow 5 1 air valves 5 1 check valves 5 1 operation 5 8 software 5 6 specifications 5 2 Controller front panel label 1 8 keypad 3 9 rear panel connectors 1 10 Cooling heating jacket 2 11 Cutting tubing 2 2 D DASNET protocol 7 6 Displays setting the contrast 3 5 Drain tube 2 3 E Electrical connections 1 13 analog control 3 17 controller 1 10 pump 1 12 Error messages serial command 7 29 External control 3 17 analog 3 6 DASNET 7 6 pinout 7 2 remote run stop 3 19 serial commands 7 15 serial error messages 7 29 serial interface 6 1 7 1 F Flow rate limit 3 11 H Help key 3 12 I ID numbers 3 6 In line filter accessory package 2 9 K Keypad acc c

HLf Series Syringe Pump User Manual

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