Dear ABI Prism™6100 PrepStation User,
Contents
1. Method Version Version of methods that is running on the 6100 prepstation It should always be the same as the Download Version unless there are problems in the system If this field is 00 00 then the methods were lost or corrupted Download Version Version number of the firmware application currently running This firmware is downloaded to the system through the firmware download serial port If this field is 22 22 the application is not available Checksum This checksum serves as a check for the application firmware to ensure that the correct firmware is running It is a double check in case the application version reported is misleading or incorrect If this field is blank the bootloader is running Firmware Type If the Firmware Type Is Then 6100 the application firmware is running on the 6100 prepstation BOOT the bootloader is running on the 6100 prepstation This means that the application firmware was lost due to a hardware error or system error No screens will be showing on the 6100 prepstation The LED at the instrument s rear will be blinking at the rate of 2 blinks second 3 Check the Firmware Type field If the value is BOOT you must download new firmware 4 Click OK to return to the Utility Application screen Firmware Upgrade 11 13 Error Messages The following table lists firmware upgrade error messages a description of the Ta2. download an older version of firmware than is currently running on the 6100 prepstation see About Downgrading on page 11 15 Connecting the A serial cable is provided with the 6100 prepstation erial Cable l gt i To connect the serial cable Step Action 1 Attach one end of the cable to the COM 1 port on your PC 2 Attach the other end to the firmware download serial port at the rear of the 6100 prepstation It is the second connector from the top as shown below Firmware download port Installing Utility To install the Utility Software on your PC Software Step Action 1 Close all programs running on your PC 2 Insert the Utility Software CD in your PC s CD drive and close the drive door 11 2 Firmware Upgrade To install the Utility Software on your PC continued Step Action 3 Choose Start gt Run The Run window appears E Beni Fea ale MEM on space 4 In the Open box type E ABI6100 Utility Installer exe where E is the appropriate drive letter or browse for this file by clicking Browse Then click OK The InstallAnywhere program runs YE ABI6100 Utility ile Es PSS LeU Eie Fest et io e Pe ety Lene es Firmware Upgrade 11 3 To install the Utility Software on your PC continued Step Action 5 Click Next The Choose Install Folder screen appears E ABIGT00 Utility CProgra
3. Applied Biosystems 850 Lincoln Centre Drive Foster City CA 94404 U S A T 650 570 6667 F 650 572 2743 www appliedbiosystems com Dear ABI Prism 6100 PrepStation User Thank you for installing the latest version of 6100 firmware Detailed descriptions of the new features and protocols in this upgrade may be found by downloading from appliedbiosystems com 6100 the 6100 Users Manual revision A2 To install the new version of firmware please refer to and read carefully the 6100 users manual Chapter 11 Firmware Upgrade at the end of this letter Failure to follow the directions in this chapter may result in loss of stored methods Version 1 07 of 6100 firmware has the following new features 1 Methods The ability to create edit save and delete up to 300 new methods 2 Methods The abilty to sort methods by method name number of steps or date last used 3 Pre Defined Methods The addition of new Pre Defined Methods These consist of versions of the existing RNA isolation protocols incorporating steps to enable use of a new in protocol on column DNase reagent for the removal of any residual contaminating genomic DNA The reagent is called AbsoluteRNA Wash Solution p n 4305545 4 Pre Defined Methods The ability to copy and edit Applied Biosystems Pre Defined Methods and save them your own user name 5 Users The ability to add up to 18 user names for a total of 20 users with secure PIN numbers Users ma
4. ble message and recommended action Message Description Recommended Action No file selected Message occurs after you select a file to Switch on the 6100 prepstation power download Warning Application detected The Utility Application determined that the Download new firmware that your firmware methods methods were lost before it attempted to If the problem persists contact are lost download new firmware This may be due to Technical Support a hardware error or a system error Warning You are trying to The firmware version you are attempting to See About Downgrading on download an older version download is older than the version currently page 11 15 User developed methods will running on the 6100 prepstation be deleted Are you SURE you want to do this Yes No Troubleshooting The following table lists the problem possible causes and a check and or remedy for Table troubleshooting the firmware upgrade to the 6100 prepstation Problem Possible Causes Check and or Remedy Method version is 00 00 Methods on the 6100 prepstation were Download new firmware lost Ifthe problem persists contact Technical Support Communication error no response Serial cable is unplugged or not Check and reseat the serial from instrument seated properly cable 6100 prepstation power is not on Switch on the power Communication was broken during a Serial cable was disconnect
5. choose File gt Exit to close the Utility Application Firmware Upgrade 11 11 Troubleshooting Upgrade Problems Overview The following topics may help you troubleshoot any firmware upgrade problems Topic See Page Viewing Firmware Information 11 12 Error Messages Table 11 14 Troubleshooting Table 11 14 About the LED 11 15 About Downgrading 11 15 Viewing Firmware When you have set up the PC and 6100 prepstation and are ready to download Information firmware the PC queries the instrument as to what versions it is running before the download actually begins You can view this information To view firmware information Step Action Info The following screen appears fe ABI Gremlin Utility Application Boot Version 00 04 Method Version 01 00 Download Version 01 00 Checksum D995 Firmware Type 6100 1 After step 3 on page 11 7 in the Utility Application choose File gt Get Firmware 11 12 Firmware Upgrade To view firmware information continued Step Action 2 View the information on the screen Field Explanation Boot Version Version number of the boot loader firmware currently running This firmware resides in a Flash ROM on the system board It is responsible for checking the validity of the application firmware and if necessary restoring it from Flash or the firmware download serial port
6. ed a Check and reseat the serial download cable b Switch off the power wait 5 seconds then switch the power on again c Start the download procedure again 11 14 Firmware Upgrade About the LED The green LED at the rear of the 6100 prepstation can provide troubleshooting information as follows Indicator Meaning Solid The application firmware is running OK on the 6100 prepstation Blinking quickly Firmware is being downloaded Blinking at 2 blinks second The boot loader is running No light The 6100 prepstation is unplugged or a fuse has blown About Downgrading Downgrading to an earlier version of firmware is not recommended However there may be certain circumstances in your lab that require you to run an earlier firmware version If you do downgrade your users methods and preferences will be lost To preserve your methods Step Action 1 Before downgrading print your methods 2 After downgrading recreate the methods manually Firmware Upgrade 11 15
7. m FilesiABI 61 0MABI 6100 Utility Software Stair ee me 11 4 Firmware Upgrade To install the Utility Software on your PC continued Step Action 6 Choose one of the following If you want to Then choose the default folder click Install Recommended choose a different folder type a different drive letter for example in the window or click Choose to browse for a different folder then click Install The InstallAnywhere program installs the Utility Software on your hard drive and then displays the Install Complete screen YE ABIG100 Utility ox stall Complete STS Aare ee E anew GL ee 7 Click Done The program displays Cleaning Up and creates an ABI6100 icon on your desktop 8 Remove the Utility Software CD from your CD drive and put it in a safe place Firmware Upgrade 11 5 Copying Firmware To copy the firmware file to your PC 11 6 Firmware Upgrade Step Action 1 Insert the Firmware CD in your PC s CD drive and close the drive door 2 Choose Start gt Run The Run window appears Run k Ed Type the name of a program folder dacument or Internet resource and Windows will oper it for you Open i Bunin seperate meman s
8. ompleting Installation screen Completing Installation This may take several minutes Do NOT cycle the instrument power at this time F1 F2 F3 F4 F5 Then it displays the main menu HH MM SS Applied Biosystems MM DD YY ABI PRISM 6100 PrepStation Version 01 00 User lt ABI gt Quick Method User Log Util To upgrade the firmware on the 6100 prepstation continued Step Action 9 When you can see the main menu on the 6100 prepstation the download is complete Check to be sure that the main menu is visible When it is click OK The Confirm screen appears fe CONFIRM Es D Can you see the Main Menu on the instrument Yes Ho 10 Check again to be sure that the main menu is visible When it is click Yes The Firmware Download Complete screen appears fi Firmware Download Complete D l downloaded firmware version is 01 00 0 11 Click OK 12 View the screen on the 6100 prepstation Remote Control of 6100 Cycle power to restart in normal mode Wait 5 seconds before turning the power back on F1 F2 F3 F4 F5 13 Power off the 6100 prepstation wait 5 seconds then turn the power back on The main menu appears showing the new firmware version HH MM SS Applied Biosystems MM DD YY ABI PRISM 6100 PrepStation Version 01 00 User lt ABI gt Quick Method User Log Util F1 F2 F3 F4 F5 14 On the PC
9. pace Cancel Browse 3 Click Browse 4 Click the firmware file e g prep0100 abs to select it the only file on the CD then press Ctrl C to copy it Close the Browse window and the Run window 6 Choose Start gt Programs gt Windows Explorer Locate the folder where you installed the Utility Software If you used the default setting it was C Program Files ABI6100 ABI 6100 Utility Software Click the Firmware folder to select and open it 9 Press Ctrl V to paste the firmware file e g prep0100 abs in the Firmware folder N Exploring Firmware File Edit View Go Favorites Tools Help t BB alja E Back Fonvard Up Cut Copy Paste Undo Delete Properties Views Address D Program Files 4B 61005461 6100 Utility Software Firmware 7 All Folders x Name OO ele TM Program Files aj a prep0100 abs 362KB ABS File 6 ABI 6100 3 ABI 6100 Utility Software a bin Seem Firmware ire E resources UninstallerData Adobe Aladdin F149 EndNote E Ghost Ghostgum Paint Shop Pro 5 E Quick Time H E Visio E Ran E Recycler H E Revision of NT SP amp SG Template _F Robin s Project Seg4_Data Site_prep_Enalish Fo 1 lt gt 4 gt 1 object s 361KB Disk free space 3 07GB EJ My Computer YW IMPORTANT Do not rename the firmware file 10 Remove the Firmware CD from your CD drive and put it in a safe place Upgrading Firmware Procedure To upgrade the firmware on the 6100 pre
10. pplication screen appears again with the selected file name displayed fe ABI 6100 Utility Application File Help 1 Select Firmware File To Download Choose File HrepoiOo abs 2 Click To Start Y Bytes Remaining 370292 7 Click the Start button which turns green as you get your cursor close to it The Utility Application downloads the firmware to the 6100 prepstation On the screen the Bytes Remaining count down The scroll bar moves at a uniform rate so you can judge how long the download will take On the 6100 prepstation the LED at the rear of the instrument flashes green quickly HABI 6100 Utility Application File Help 1 Select Firmware File To Download HrepodOo abs F Bytes Remaining 346858 6 i Downloading firmware Firmware Upgrade 11 9 11 10 Firmware Upgrade To upgrade the firmware on the 6100 prepstation continued Step Action 8 Wait while the firmware completes the installation The Completing Installation screen appears fas Completing Installation Please wait while the firmware completes the installation This may take several minutes DO NOT turn power off during this process Click OK after the Main Menu on the instrument appears IMPORTANT Do not turn the power off on the 6100 prepstation Doing so will cause you to lose your methods users and preferences The 6100 prepstation displays its C
11. pstation Step Action 1 Ensure that The 6100 prepstation s power is on The procedures in Preparing for a Firmware Upgrade on page 11 2 have been performed Close all programs running on your PC On your PC desktop double click the ABI6100 icon eal Did ABIETOO 1 0 The Utility Application screen appears fe ABI 6100 Utility Application File Help 1 Select Firmware File To Download Choose File Mo File Selected 2 Click To Start Bytes Remaining Firmware Upgrade 11 7 To upgrade the firmware on the 6100 prepstation continued Step Action 4 Click Choose File The Choose Firmware Download File screen appears f Choose Firmware Download File Look in asisto0 Utitity Software C ABI 6100 CA ABI 6100 Utility Software Software cal E am EJ Firmware Ta Logs EJ UninstallerData CJ resources File name Open Files of type abs x Cancel 5 Double click the Firmware folder icon The firmware file e g prep0100 abs is listed on the screen f Choose Frimware Download File Look in CA Firmware i cl el E Ea 4 preph100 abs File name Open Files of type abs hd Cancel 11 8 Firmware Upgrade To upgrade the firmware on the 6100 prepstation continued Step Action 6 Click the firmware file to highlight it then click Open The Utility A
12. y then save new methods against their user name ensuring that their own protocols are stored safely 6 Utilities Addition of a real time clock and date display in the instrument front screen The date and time may be formatted in a number of different ways from the instrument utility page The following features are NOT included in this version but will be completed in subsequent upgrades 1 Printing 2 Run Log If anyone has any further questions or requires help with the firmware upgrade procedure please contact your local technical support personnel Best regards Andy Felton Product Manager Sample Preparation Systems Firmware Upgrade Overview About This Chapter This chapter explains how to upgrade the firmware on the ABI PRiSM 6100 Nucleic Acid PrepStation Your 6100 prepstation has firmware loaded when you receive it This chapter describes how to upgrade to a later version of firmware when it becomes available In This Chapter This chapter contains the following topics Topic See Page Preparing for a Firmware Upgrade 11 2 Upgrading Firmware 11 7 Troubleshooting Upgrade Problems 11 12 Firmware Upgrade 11 1 Preparing for a Firmware Upgrade Overview Preparing to upgrade the firmware consists of the following procedures Procedure See Page Connecting the Serial Cable 11 2 Installing Utility Software 11 2 Copying Firmware 11 6 IMPORTANT Ifyou are attempting to
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