RheoSense Manual Ang
Contents
1. Q is the flow rate w the width of the channel h the depth of the flow chan nel and 7 the measured wall shear stress In order to apply the correc tion a data set of Ra and 7 are needed VROC_RateSweep is designed to measure the data set and do the correction at the end of the measure ment Caution The more accurate the collected data set the more accurate the correction will be If data is outside of the measurable range the cor rection can be erroneous If the correction is erroneous it is recommended 6 04 PM Board Temp oC 2 15E 01 2 15E 01 2 15E 01 2 15E 01 Sensor ID Date 25 Sensor Temp oC 2 16E 01 2 6E 01 2 16E 01 2 16E 01 2 025 mm Pa 2 34E 00 2 08E 01 2 08E 01 2 08E 01 to do the procedure manually 18 4 525 mm Pa 1 09E 01 2 82E 01 5 12E 01 5 17E 00 8 325 mm Pa 1 21E 01 1 76E 01 1 06E 00 1 76E 01 60 h a x 2 Apparent Shear Shear Rate 1 s Stress pa 2 67E 03 2 61E 02 2 67E 03 8 67E 03 2 67E 03 4 87E 02 2 67E 03 2 58E 03 d In R d In 7 Apparent Viscosity Pa s 9 79E 06 3 25E 06 1 83E 05 9 68E 07 12 VROC_RateSweep Program The VROC_RateSweep program measures the viscosities at multiple shear rates defined by the user within a single test run The program automati cally scans all the inputs shear rate number of data points sensor informa tion available volumes to guide a user in the b2. Sensor ID User will be prompted to select the sensor file after the pro gram starts The serial number is located on the back of the VROC Each chip comes with its own sensor files calibration files which need to be loaded onto the program for accurate viscosity measurements The sensor file only needs to be loaded once onto the program until user switches to a different chip Click the New Sensor button before the next run for a new sensor chip Pump Port Serial port for the communication of the pump Syringe diameter mm Custom D Sample Volume ul Max Pressure Pa Sampling time ms Flow rate Dispensed volume ul Available volume ul 500 ul z 3 256 500 0 20 Infusion ulmm Y 10 0 19 Syringe Diameter mm For the syringes supplied by the manufacturer choosing the size of the syringe from the drop down menu will automati cally input the diameter For a custom syringe enter the diameter by choosing custom from the drop down menu and enter the value in the Custom Dia field Sample Volume ul Enter the available sample volume for the measure ment before the measurement starts The program will automatically up date the available volume after each measurement This value should be entered accurately in the beginning to prevent damage to the syringe Maximum Pressure Pa Displays the maximum pressure range of the sensor The program s build in safety features monitor the leading sensor and prevent pressure fr
3. 0 o Rate Screening off New protocol Results User value i MU Number T Board T Sensor C DP Pajmm Flow rate l min 5 R 1 5 5 5 Pa Wis Pa s c 5 R 1 5 cvis Pa s A T Flow rate ulmin 5 seconds 3 seconds F J T T 31 a T T ulfmin or 15 j gozon ds Waiting time s j I PA 20 secon ds 100 ee ond 300 10 seconds oo ud seconds I AE z d Foo Jad 50 seconds x EA 60 second a El ir Preset Custom s R viscosity h al Viscosity vs Shear rate ae corrected viscosity n 6 0 1 000E S A TETTE 4 0 2 0 e 0 0 H E 2 0 Sl 4 0 a 3 amp 6 0 2 8 0 10 0 12 0 i 1 y 1 1 1 1 13 1 1 1 T 1 1 000E 6 Y 1 1 20 25 30 35 40 45 50 55 60 65 70 75 80 85 100 0 1000 0 10000 sensor position mm shear rate 1 5 Waiting Time sec The time needed to reach a steady state for a viscos ity measurement Because of a slight compressibility and small yet finite dead volume reaching the steady state viscosity may take time It could take a few seconds or more than 10 seconds depending on the pumping flow rate and viscosity of the sample If the sample has air voids and the void volume is significant it could take much longer 21 The user is encouraged to use the VROC_SP_TRANS to find out the neces sary waiting time for each sample Clicking on the button will display avail able pre set times and custom times for user input v
4. mo ascos aero seem seca omo ascos Parser oren amasar amo eaen 20001 2 amcor us rencor toreo one zace 35 VROC C 05 Full Scale Pressure Pa Flow Channel Gap um Flow Channel Width mm LR INN S s oa rre rasos erez amena 108 as iren ravens smen omens vom aa ao ameno erens arer eaves e ote e rre rre so sones te e reos sacra eareor arena re ome ames roo sme smc ima com au arcos sano area oreo vom Tm oo ea veo area eme ZI omu ao rmen aries son ceace a aro ameno arica asen eres a o esse aeeoe asco aos lt on MOE recor A o meoo neoe araeo oreo oa omo ameo rreo soo seaca O oa Teno omeo seco aareoa arco lt won com Pasco esco owen sco f om 36 VROC C 10 Full Scale Pressure Pa Flow Channel Gap um Flow Channel Width mm T IN mPa s Co esco a e area om os ime ames serena eses sm as roms samo asco asen ame a ineo reos oxen omens e reo onmens zres ameno sow es ases 1000 James se rs raze asez asen e aie rn reos onen oaeo se za aa re 100000 ascos ano te e aa omen iew aeo ano ama ze oo ramos romeo essen eses rom ou ao an 2200 amen meo Zau aaa amen 152502 aseos eceror lt on omo mea rene amenn awe lt to com onen o
5. oreo areo carea lt rom eo acon seson zoren zoro lt on aro zmen oeo ame 200 roo oo amcor ames ss ameno _ amo cos or amen emo ooo arcos arica avem aren omo aeo aos ameno zoe omo zoen zace 210609 amo um osen zoe asco ima JO 29 VROC A 10 Full Scale Pressure Pa Flow Channel Gap um Flow Channel Width mm ere e Pe e e Pe E s oe ruen Paes omer omeo se za oo roen ros 2002 22004 se am oo sacre coses 172502 rez 250 ua ra rose oaeo amos com 290 o soen seso error aros ama te ea reo rosca zos amos ou zau on sooo sanz 1720 leg room ou ao aa 2200 amen seo Zac ao mn ieo ome omen a E 1000 amen ae rac aro soen soo aren ire E TA ooo asen essor mean sn om amo meor zo ooreo sarea lt rom aa ieor 110501 oeo oaen lt rom eoo ran 130000 aaen zare lt on ooo seoeo2 sooroo 472002 zero rau om assen aseo ore e 30 VROC B 02 Full Scale Pressure Pa Flow Channel Gap um Flow Channel Width mm e e Pe Pe e Pe mPa s os essen oseo ieo amen zau te as ozen casos 150600 ez ma a oo aora sones socio seen ma ma o E H e nara sore soen sec a o onen oren asen cnc acu ame oe amen amos vo a ae osse peat veo omu au fermen se rsen A au s
6. 1 ml Custom Dia Sample volume ul Max Pressure Pa 3 256 js00 0 9 of LS Pitch J A Sampling Time ms 120 125 Jis 2 5 ml I 50m 10 0 ml 2 250m Custom mm Measu time sec L Number to Ave X 1 0 101 900 0 Pumping Rate ul min Delivered Volume Units Sanal for Ave sec 0 0 0 0 AveSs 0 Plot 0 Number of Data Points Number of averaged data Shear Rate 1 5 Pressure Drop Pajmm 2 35 o 0 0 0 0 S E 2 25 za i 2 2 Pressure vs positions 5 E 2 15 21 800 0 0 101 700 0 O Average Vis 0 Plot 0 600 0 Z 500 0 400 0 200 0 200 0 a is ll ll rl S CE ELE tere 20 25 90 95 40 S S0 5S 60 6S 70 7S 60 6S 095 J00 10 9 0 Positions mm Time s 3 start NO m4 windo S Skype r Sensor Int S ta VROC_She VROC_SP_ Rheosense Va VOL CRE ms 2 51PM Sample Volume ul Enter the available sample volume for the measure ment before the measurement starts The program will automatically up date the available volume after each measurement This value should be entered accurately in the beginning to prevent damage to the syringe Maximum Pressure Pa Displays the maximum pressure range of the sensor The program s build in safety features monitor the leading sensor and prevent pressure from overloading the sensor The green light next to the Max
7. small periodic pulsa tion which could be large enough at low shear flow rates In order to maximize the measurement accuracy the software calculates a period of the pulsation at each flow rate and averages the measured values accord ingly If averaging over multiple lead screw pitch is desired then type in desired number One lead screw pitch is sufficient in most applications Displays Pumping Rate ulmin Delivered volume Units signal for Ave sec 0 0 0 Number of Data Points Number of averaged data Shear Rate 1 s Pressure Drop Pafmm 0 0 0 0 Pumping Rate ul min Displays the actual pumping flow rate in ul min Delivered Volume Displays the delivered volume in ul Number of Data Points Displays the total number of data points for the measurement Total Number of Averaged Data Displays the total number of aver aged data points Shear Rate 1 s Displays an apparent shear rate value assuming the test solution is Newtonian For non Newtonian the Weissenberg Rabinowitsch correction is applied See note A Pressure Drop Pa mm Displays the pressure drop measured by the sensors 15 Temperature oC Sensor 16 Signal for Ave sec Period of one pitch of the lead screw in seconds Pressure vs 230 0 2 0 3 0 4 0 5 0 6 0 7 0 8 0 3 0 10 0 11 0 Positions mm Pressure vs Positions Displays measured pressures at different loca tions The curve should be linear at least on ave
8. the chip to the third port of the valve as illustrated Arrange the position of the switch on the valve as illustrated in the pic ture below and operate the pump manually please refer to the user s manual of the pump to dispense a small portion of the test liquid to the waste This procedure effectively removes air bubbles inside the sy ringe and the tube Caution Do not operate the pump manually when the valve is connect ing the syringe to the chip 10 To Chip To Syringe Tube 1 4 Position of the switch indicates a flow path from the syringe to the waste After removing the air bubbles arrange the position of the switch valve as illustrated in the picture below to measure the viscosity To Syringe Position of the switch indicates a flow path from the syringe to the chip 8 Orientation of the Chip The inlet and outlet are recommended to be placed horizontally at the same level Configuration 9 Cleaning the Chip after Usage Always use the provided software package with the VROC chip for any testing and cleaning Users should be aware that drying of a sample solution inside the chip af ter a prolonged period of inactivity carries a high risk of permanently clogging the chip preventing normal fluidic flow inside the chip To avoid clogging of the chip it is important that the user cleans the chip with the appropriate solvent to remove sample solution after usage The recommended pro
9. 5 RHEOSENGE INC VROC Viscometer Rhneometer on a Chip User s Manual ON OO eS Table of Contents Unpacking Precautions Hardware Setup Sample Loading Loading the Syringe on the Pump Chip to the Syringe Connection Removing Air Bubbles Orientation of the Chip Flow Path Cleaning Software Setup VROC_SP_TRAN Program VROC_RateSweep Program Appendix Front Panel Functions Appendix Il Guidelines for VROC Cleaning Appendix Ill Ordering VROC chip and Syringe 1 Unpacking Check the package for any missing components Control Box DC power supply for the control box 110 V only RS232 cable for the control box Connector for the control box and VROC chip interface VROC chip Syringe pump USB to Serial port converter 2 Power cord for the syringe pump RS232 cable for the syringe pump Software CD 2 Precautions 9 CAUTION Always use the provided software package with the VROC chip for any testing and cleaning The programs have build in safety features that prevent pressure from overloading the chip Always clean the chip thoroughly after each application and before storing the chip Please refer to section nine for clean ing procedures Particles larger than 10um should be filtered to avoid clog ging Do not spill or allow any liquids inside of the enclosure of VROC chip It should be kept dry at all times Note
10. alue Display results Pressure vs Positions 2 0 2 5 3 0 3 5 4 0 45 5 0 5 5 6 0 6 5 7 0 7 5 8 0 8 5 sensor position mm Pressure vs Positions Displays measured pressures at different locations The curve should be linear at least on average to measure a meaningful viscosity All the pressure profiles at different shear rates are displayed in the graph above Results T Board C T Sensor a DP ee Flow rate el SR Cs 0021 sze fo 0000 0021 222165 223236 fosset amooo 157 7018 2 5203 22 2222 151 7243 0021 a Ea a EE AA AN AAN A A EE RS Results Displays the results including board temperature T Board sensor temperature T sensor some sensors do not have this feature pressure drop DP Pa min flow rate ul min shear rate S R 1 s shear stress S S pa viscosity Pa s true shear rate corrected shear rate and true viscosity corrected viscosity 22 viscosity Viscosity vs Shear rate seers Sass viscosity pa s l l 100 0 1000 0 10000 0 shear rate 1 s Viscosity vs Shear Rate Displays all of the results of the viscosity meas ured at all the shear rates Data and Report Saving At the end of execution user will be prompted for data and report saving In the prompt the location of temporary re port file in html will be disclosed and the same report will be printed to a defaul
11. cedure consists of the following steps 1 Connect a sol vent filled syringe to the chip 2 Very slowly infuse about 100ul of sol vent to remove majority of the sample solution out of the chip flow rate should not exceed the maximum flow rate used during measurement 3 Increase the flow rate and clean the chip as many times as necessary de pending on the sample solution 4 Finally test the chip with a known sam ple for viscosity to verify that the chip is clean Please see Appendix for more on the suggested cleaning procedure If clogging persists do not attempt to force a flow as it may cause perma nent damage to the chip If there is any signs of clogging please contact the manufacture for a possible solution 12 10 Software Setup a Create a folder in the C drive in your computer and make a copy of all the programs drivers and the two sensor files that come with your software installation CD b WROC_SP_TRANS This program measures the viscosity at the specific shear rate defined by the user lt only works with a specific pump supplied by the manufacturer c VROC_RateSweep This program measures the viscosities at multiple shear rates defined by the user within a single test run It only works with a specific pump supplied by the manufacturer d LVRunTimeEng LabView program driver Install the program NI VISA Serial ports driver Install the program 11 VROC_SP_TRAN Program Type in values for th
12. e Loma eerror rom IE E tu amo omeo oreo aren ars lt rom o asar esco sare rare lt rom so omeo 200501 oaeo oreo won omo zaen zone mea coreo lt rom com noaeo recor seoa soe O om 33 VROC B 20 Full Scale Pressure Pa Flow Channel Gap um Flow Channel Width mm ERIC INN un s os aee ome toreo vores me as omen sones reno roses ame as esse aos seno ames ame si aseos zene ames ame rr mesos 210600 ares sow rones omens sore sss ze o sacre somos rie ros e ae esse aos soc soo toe som aa omen rior mea e se aa nen a soen sea re e am sarma sence reno reo som sou eo ESET soco RE re S oa Teno aneor ateos soen seo a omn osen oseo aen ee os 34 VROC C 02 Full Scale Pressure Pa Flow Channel Gap um Flow Channel Width mm EI III ua s o aaron estes sonor osos me e a5 omen aseos areo area tee se os zoo zarea aero ame ama ze eg onze aren areo rom o a mea zoo zare eeo omu zau a aa cerere pan seo E o o omen ore ome arcos a a amen ee amen 200501 a o area sore aman amcor lt rom ao oseon HEH lt rom ao aia cerere soewe soe lt oa aro eo areo amo H ono aaen zoea amoo ewe lt ro mo ao vera R amo soso oseo vem ie
13. e controls VROC 9 99 Measurement is completed Sample ID Viscosity Range cp Baud Rate Sensor Port prse Sensor ID x Pump Port Ne Sensor STOP 115200 R 8VA05100020Low 18 200000 Y sof 115200 R 34 8000000C Sw E Initialization SD Syringe diameter mm Custom Dia Sample volume ul Max Pressure Pa tm bias A 117000 y of LS Pitch Parameter Flow rate ul min v Measu time sec y Number to Ave z manne 1 2000 6 125 dis 4 Initialization Select this button to initialize the system STOP Pressing this button will stop the ongoing measurement Sample ID If this field is empty user will be prompted to enter the ID of the sample while the program is running Viscosity Range cP Estimated viscosity range of the test sample This value is used to pre calculate the maximum flow rate and correspondingly gives a warning to the user if the flow rate exceeds the maximum value Estimated high end values of the viscosity of the test sample should be entered as accurately as possible to minimize the potential damage to the chip Baud Rate Baud rate of the serial port lts value should be larger than 57600 Sensor Port Serial port for the communication of the VROC control box COM1 or COMX Available port can be found in the start control panel system hardware device manager ports Baud rate of the avail able port can also be set at this location 13 Pump Port Serial port for the co
14. ed to the com puter e Turn on the power for the control box and the syringe pump 4 Sample Loading Recommended procedure for loading sample into the syringe Use the plastic needle included to load the sample solution into the sy ringe Air bubbles can be generated during sample loading Remove them as much as possible by tapping the syringe gently with the needle pointing upward and pushing a small amount of sample solution out Air bubbles can be trapped inside the flow channel of the chip causing non linear pressure profiles due to distorted flow fields They will also delay the response time and be a source of error in viscosity measurement as the bubble is compressed when pumping starts Troubleshooting to remove trapped air When air bubbles are trapped inside the flow channel usually higher viscosity samples can be used to remove them For example air trapped during water injection can easily be removed with isopropyl alcohol which is twice as vis cous as water To effectively remove all the air bubbles please refer to section 7 5 Loading the Syringe on the Pump Note Do not connect the syringe to the VROC chip before this step a First unlock the drive nut knob as shown n the photo Drive nut kn Unlocked position Pusheriblock b Load the syringe onto the syringe pump c Slide the pusher block along the guide rods until the block presses firmly against the syringe plunger Rotate the k
15. en sore soem seco rom o onem oreo asen caca a ao rare ioeo zece oe H am oseo oseo eo eo ao ezen ozem rsen ree am os aereos sen sme mo arcos oreo sn aseos amo ronca ioeo zac aco ua oscar neo vnc omo sacos case rsen ree omo aster sere soene seem gt gt im arcas aren ascos asar 31 VROC B 05 Full Scale Pressure Pa Flow Channel Gap um Flow Channel Width mm EII mPa s ese ames eee See oe ae as rsen isens 150002 era se a a reens tonos oros oren e na es agn 100501 seno a ie sz tonos ss arena ma to roo aes 150500 sa oou ooa a aa 100 aran eno A om o oem H os E E lt om ao aaa H lt om aro seno 12 ya ros os om on ce omeo orce som ao oaeo ozon O ou aw ameo cor aaen ass lt os aaa H H S rom omo tosco ieo sree loss amo reo ieor oreo omen im ozen vasco omem omen 32 VROC B 10 Full Scale Pressure Pa Flow Channel Gap um Flow Channel Width mm EAN III AE s aa esse assis vara vores ze oo aons amas oreo omeo ze so omens eases ooren saven me aie e ocna amen omeo orcos 290 ms o aos 220 coreo mre e ae n amos amen aves orco zau e ao zonas 2200 cores are mu com oo oca soc amen ene E o ono aao 22
16. end of the measurement The file will have the extension ptc User value YF When this menu is selected user inputs flow rate measurement time wait ing time on the screen panel Retrieve protocol When retrieve protocol is selected the user can use existing protocol for the measurement This feature is useful for repeated measurements Flow Rate ul min Enter the flow rates at which viscosity needs to be measured One can also choose shear rate 1 s using the drop down menu Measurement Time sec Enter the desired duration of measurement of viscosity It is recommended that enough measurement time be allocated Or select the time using the drop down menu If preset is selected the minimum measurement time calculated based on the lead screw pitch will be used for the optimum measurement preset is recommended for most applications VROC_ShearRateSweep3 1 5 vi Front Panel Oleaje File Edit view Project Operate Tools Window Help gt 2 ZI 15Pt Application Font gt 2 ia 2 5 E E 3 y E WROC Chip New Sensor Sensor ID Pump Viscosty range mpas HE p Hop Sampler iscosity range mPa veral Baud nae lt A pump Port com A ma om Syringe diameter mm Custom D Sample Volume ul Max Pressure Pa Sampling time ms Flow rate Dispensed volume ul Available volume ul 500 ul i 3 256 500 0 20 Infusion Rate ulimm
17. est use of the VROC M Enter the values for the controls Viscometer on a Chip O 595999 VSS Chip New Sensor Sensor ID Pump Newtonian Viscosity range mPa s Sample ID ba ity range Sensor Port BaudRate ii gt Pump Port 1 li coma 115200 lt mua from F STOP Pressing this button will stop the ongoing measurement Sample ID If this field is empty user will be prompted to enter the ID of the sample while the program is running Newtonian i non Wewtonian The user can select Newtonian or non Newtonian before the program runs If non Newtonian is selected Weissenberg Rabinowitsch correction is ap plied automatically at the end of the measurement At the end of the measurement the user will be prompted again for the correction Viscosity Range cP Estimated viscosity range of the test sample This value is used to pre calculate the maximum flow rate and correspondingly gives a warning to the user if the flow rate exceeds the maximum value Estimated high end values of the viscosity of the test sample should be entered as accurately as possible to minimize the potential damage to the chip Sensor Port Serial port for the communication of the VROC control box COM1 or COMX Available port can be found in the start control panel system hardware device manager ports Baud rate of the avail able port can also be set at this location Baud Rate Baud rate of the serial port lts value should be greater than 57600
18. imum Pressure textbox turns red when the leading sensor experi ences a pressure higher than its maximum pressure rating Beyond this point should the pressure continue to rise the program will automatically stop to prevent potential damage to the chip 14 Flow Rate ul min Enter the pumping flow rate in ul min The pump will be set for the specified flow rate If this value exceeds the maximum value calculated using the estimated viscosity the user will be prompted to lower the flow rate only once Shear rate s Shear rate can be selected instead of the flow rate from the drop down menu Flow rate is determined using the shear rate Measu Time sec Enter the total duration of measurement In addition there is a small ripple effect associated with the pumping system In order to remove this ripple effect it is recommended that enough measurement time be allocated If the entered measurement time is lower than the re quired time the user will be prompted to increase the time only once Number to Ave Number of sample data to average in order to reduce the random noise The extent of the noise is inversely proportional to the square root of the number of average Therefore the larger the number to average is the smaller the noise will be Sampling Time ms Sampling time in milliseconds This is the interval between measurements Recommended minimum sampling time is 15 ms of LS Pitch A lead screw based pump exhibits a
19. mmunication of the pump Sensor ID User will be prompted to select the sensor file after the pro gram starts The serial number is located on the back of the VROC Each chip comes with its own sensor files calibration files which need to be loaded onto the program for accurate viscosity measurements The sensor file only needs to be loaded once onto the program until user switches to a different chip Click the New Sensor button before the next run for a new sensor chip There are two sensor files created for two different tem perature ranges The file with a suffix LT for example 7VB1100040_LT is for the lower temperature range 18 C 35 C whereas the file with a suffix HT for example 7VB1100040_HT is for higher temperature range 35 C 50 C Syringe Diameter mm For the syringes supplied by the manufacturer choosing the size of the syringe from the drop down menu will automati cally input the diameter For a custom syringe enter the diameter by choosing custom from the drop down menu and enter the value in the Custom Dia field VROC_SP_TRANSv7 1 vi Front Panel File Edit View Project Operate Tools Window Help gt a a n 13pt Application Font TESES j VROC oes Sample ID Viscosity Range cp Baud Rate Sensor Port Hansa Sel Pump Port STOP 1 115200 lt Become a 2 com Y NN Initialization Ca Board A 100 Board 0 Sensor 0 Ave TO Sensor 250 ul 500 ul
20. nob to lock the drive nut Any slight gap between the pusher block and the syringe plunger could lead to a longer time for a steady state for viscosity measure ment To ensure the intimate contact between the pusher block and the syringe plunger press both buttons of Run Stop button and gt and hold for a very short duration Before pressing these two buttons make sure that VROC chip is not connected to the syringe Please refer to the User s Manual of the pump page 8 for loading the syringe in detail 6 Chip to Syringe Connection Remove the plastic needle from the syringe and connect the flat bottom fitting from the chip to the syringe Finger tightening is sufficient Note Do not connect the chip until the syringe is loaded on the pump Step 5 Connection to a syringe Sold separately Minimize tubing length by positioning the chip at the same level as the syringe See above figure 7 Removing Air Bubbles with a Switching Valve Optional but Recommended After loading the sample into the syringe connect the tubes to a switch ing valve as illustrated in the picture below Two different tubes are supplied with the valve one with fittings on both ends Tube 1 and the other with only one fitting Tube 2 First connect the black fitting of Tube 1 to the valve and then connect the beige grey fitting to the sy ringe Second connect the black fitting of Tube 2 to the valve Lastly connect the beige grey fitting from
21. om overloading the sensor The green light next to the Maximum Pressure textbox turns red when the leading sensor experi ences a pressure higher than its maximum pressure rating Beyond this point should the pressure continue to rise the program will automatically stop to prevent potential damage to the chip Sampling Time ms Sampling time in milliseconds This is the interval between measurements Recommended minimum sampling time is 15 ms Flow Rate with Unit Displays the flow rate with proper units Dispensed Volume Displays dispensed volume in ul The dispensed vol ume will be updated after each measurement Available Volume Displays available volume in Ul The available volume will be updated after each measurement Rate Screening off New protocol User value Y Select parameter Measure time s Waiting time s Flow rate ulfmin SOseconds 3seconds gt de 100 10 3 300 10 3 SOD 10 3 700 10 3 Rate Screening off Rate Screening This feature helps user to set the program so that the program pre calculates if the input flow rate or shear rate is either too small or too large for reliable data The calculation is based on the user input of the maximum sample viscosity This feature can be turned off if the protocol is well established Otherwise it should be turned on New protocol New Protocol When this button is on green the user will be prompted to save the measurement protocol at the
22. osity General use viscometer BO2 BO5 B10 0 2 cP 2 000 cP Sufficient amount of sam ple for low viscosity sam ples C chips High viscosity low viscosity samples CO2 CO5 C10 0 2 cP 10 000 cP high shear sufficient sam C20 ple required High viscosity samples 26 Estimate the viscosity range of the test sample Determine the amount of available sample volume Determine any special requirement on high shear rate SS a Refer to the Shear rate and Flow rate tables on the following pages and find the chip and syringes that meet your application For example To test ink at high shear rate 1 ink viscosity 4 cP 2 Amount of sample available unlimited 3 Shear rate requirement 1 0x10E5 1 s 4 Refer to the Shear rate and Flow rate tables and find for the proper chip based on viscosity shear rate requirement and sample amount Sometimes more than one chip can meet the requirement Both A and B chips cannot meet the requirement for high shear rate Now looking at the C Chips both CO5 and C10 chips are capable of reach ing the required shear rate For the CO5 chip maximum flow rate and minimum flow rate are 8 73cc per min and 0 087cc per min respec tively The recommended syringe size is 10cc for the maximum flow rate range For the C10 chip maximum flow rate and minimum flow rate are 68 7cc per min and 0 687cc per min respectively The rec ommended syringe size is 50cc The 50cc syringe
23. ple Volume ul Max Pressure Pa Sampling time ms Flow rate Dispensed volume ul Available volume ul 500 ul sa 3 256 387 4 Q 110000 20 400 0000 ulimn 73 9 387 4 Rate Screening off New protocol Results User value Number T Board C T Sensor C DP Pafmm Flow rate uljmin S R 1 s 5 5 Pa Vis Pa s c 5 F A Select parameter Measure time s waiting time s 1 00000 23 39427 23 00000 0 46947 100 00000 1326 68647 0 01139 8 58806E 884 3seconds da 7 2 00000 23 38131 23 00000 0 63242 300 00000 3980 05942 0 01535 3 85627E 2652 3 00000 23 36026 23 00000 1 35772 400 0000 5306 74589 0 03295 6 20918E 3537 A ve H corrected viscosity pressure Pa amon aw a O an w y 0 1 1 1 1 1 i U 1 1 1 1 1 1 1 1 20 2830535 410 45 5 0 55 00 65 20 39 90 85 1000 0 sensor position mm shear rate 1 5 File Tab e Open open program e Close close program Close all close program a Page setup printer set up choose printer and page set up for print ing Print windows print front panel e VI properties general location of the program Exit close program Edit Tab e Reinitialize to default change all the control settings back to default values 24 Operate Tab e Print at completion print front panel upon comple
24. rage to measure a meaningful viscosity The slope of the curve is the Pressure drop Board Board 22 33 Sensor 25 23 Temperature oC 4606 4 06 Temperature C Displays the temperature of the board and the sensor Certain types of chips may not have the sensor temperature included and it will show a constant temperature e g 25C or 23C Shear Stress Pa 76 21666 Pa 4606 4 06 Shear Stress Pa Displays the wall shear stress measured in Pa Viscosity Pa s 0 00206186 Poto PA 0 315 29 933 Time 5 Viscosity Pa s Displays the apparent viscosity measured with time scale seconds In order to obtain non Newtonian viscosity rigorous Weissen berg Rabinowitsch correction should be applied see note A With the VROC_RateSweep program the correction can be performed selectively Data and Report Saving At the end of execution user will be prompted for data and report saving In the prompt the location of temporary re port file in html will be disclosed and the same report will be printed to a default printer Click OK to save a copy of the files and print The saved data has the following information VROC VROCW24D3I_3st 8 30 2006 Number of Data For Averaging Flow Rate ml min 0 050000 Time s 3 75E 01 7 50E 01 1 13E 00 1 50E 00 Note A Weissenberg Rabinowitsch correction R Newtonian wall shear rate R corrected wall shear rate
25. ren orem arscor ms 37 VROC C 20 Full Scale Pressure Pa Flow Channel Gap um Flow Channel Width mm ele lee mPa s omo omen rares smear we a estos eases Teen eee ome as ioeo aos oseo aseos ame so zarva azere aoe asen e ocru aro zrem amos ame 2 rones ames tores vores sme esse aos sera soes ame o asi aseos aseo aseos om es aos zooo zones ame o ar eos arena ams sme n seco oara vores ros se aa onen asnos aser oseo se za au ozen zancos 200502 aceea se za oo aos zoe ases aseos com 290 38 VROC C 30 Full Scale Pressure Pa Flow Channel Gap um Flow Channel Width mm Shear rate 1 s Flow rate l min Recommended Syringe Size Viscosity mPa s 2 0 E 04 A Of 6 98E 04 6 98E 06 1 28E 04 3 49E 04 1 74E 04 6 42E 03 4 28E 03 1 16E 04 3 21E 03 8 7 2E 03 2 0 E 03 6 98E 03 3 49E 03 6 42E 02 1 74E 03 4 28E 02 1 16E 03 3 21E 02 8 2E 02 gt STEN mz 1 28E 02 3 49E 02 6 42E 01 1 74E 02 4 28E 01 1 16E 02 9 2 1E 01 8 72E 01 C 2 57E 01 6 98E 01 cc 1 28E 01 3 49E 01 500ul 6 42E 00 1 74E 01 250ul 4 28E 00 1 16E 01 250ul 3 21E 00 8 72E 00 100ul 2 57E 00 6 98E 00 100ul 1 28E 03 39 40
26. should be used with a HP High Pressure pump CO5 Shear rate 1s rate 1 s Flow rate l min dio tr Syringe Viscosity a s 1 1 16E 03 1 16E 05 8 73E 01 8 73E 03 oc Note Minimum flow rate is 1 07ul min with 100uL syringes Maximum flow rate is 31890ul min with 25cc syringes Maximum flow rate is 75000ul min with 50cc syringes only with HP pump 27 VROC A 02 Full Scale Pressure Pa Flow Channel Gap um Flow Channel Width mm TI Mina ESA PE a ia a aa 234E 00 2346 02 282802 28280 1S0E 00 156E 02 188E02 188E10 147400 1A75 02 141802 141610 o 9368 01 936E 01 119602 11880 N EN A A ae es A A A IE 4 0 2 0 4 1 2 4 10 20 40 100 200 00 800 so enter ames aeos teo gray values smaller than min flow rate orange values larger than max flow rate with 1 syringe configuration E blue values can be achieved by using HP pump and or 50cc syringe 28 VROC A 05 Full Scale Pressure Pa Flow Channel Gap um Flow Channel Width mm ere III 2 s Ta aren T aen rene e oe os omen oar aster zos com 25 oo rmen 209 zeo zecea mu 290 osos assess mesa oreo vom zau a osos ae zero 200502 ima zau a aa 1000 area eno Erau a oen EZ lt rom au ee o V oa o eo zoe ame ze ooa o aaro ae areo reo oa ao neemo ioeo oreo oreo a ao armen
27. t printer Click OK to save a copy of the files and print Note Using the VROC_SP program completely fill the VROC chip flow channel with test sample no bubbles inside the channel before making the baseline measurement for the evaluation test This is a very important step to ensure accurate baseline measurements A partially filled channel will give an erroneous reading of baseline If there is liquid inside the chip make sure that at least 80 ul of sample is pumped to the chip to replace the liquid with the test sample If there is enough sample 150 ul elution volume is needed Appendix Extra Functions on Front Panel File Tab e Open open program e Close close program Close all close program e Page setup printer set up choose printer and page set up for print ing e Print windows print front panel e VI properties general location of the program Exit close the Edit e Reinitialize to default change all the control settings back to default values 23 Appendix Extra Functions on Front Panel E VROC_SF atc eps y tens V File Edt Operate Tools Window Help 0 3 3 25 2008 10 33 23 351 AM VROC TEET Measurement is completed DEEN WROC Chip New Sensor Sensor ID Pump non Newtonian Bg viscosity range mPa s STOP Sample 1D yaua Sensor Port Baud Rete NN 7 A5100069High Pump Port 1 come 115200 y 34 800000 49 4000000C com As Syringe diameter mm Custom D Sam
28. that the O rings are not sealed to the enclosure nor the fittings so they do not prevent leakage Keep the inside of the tubes and the flow channel of the chip wet at all times Drying could cause irreversible damages to the chip if the chip is not thoroughly clean Always leave the tubes connected with the chip Removing the tubes exposes the sensor area inside the chip and may cause damage or clogging Do not tighten fittings unless they are loose Over tightening may damage the chip Do not apply too much bending force to the Teflon TFA tube 3 Hardware Setup a Control Box Rear panel connection Rear panel Connect RS232 connector and DC power RS232 DC power CAY The other end of the RS232 connector is connected to the computer b Control Box Front panel connection Front panel L On Off Switch Connector to chip Align the circular connector by gently pressing the plug of the circular con nector against the receptacle and rotating the plug until a soft click sound is heard Then pull the spring loaded silver rim back while pushing the plug into the receptacle Finally release the rim to lock the connector in place c Connection to the chip Plug in the connector to the chip d RS232 connection to the pump Connect the phone jack type TTL connector of the Pump RS232 to the IN port on the back panel of the syringe pump The other end of the serial port connector is connect
29. tion of a run e Log at completion control values in the front panel will be logged upon completion of a run e Data Logging Log log the control values If not logged a prompt for a new file name will appear O Retrieve the following screen below will be displayed Select the desired log file to retrieve Control values saved in the same log file are saved in sequence and can be re trieved Change log file binding change to different log file name O Clear the log file binding clear the binding to the log file created Retrieve is no longer available after the binding is cleared Tools Tab newer program only Rate Sweep 3 1 5 and VPv7 1 e Security e Domain account manager 25 Appendix Il GUIDELINES FOR VROC Cleaning Low viscosity Medium viscosity High viscosity Below 100cp Above 1000cp 5cc flushing 10 to 20cc flushing 20 50cc flushing e Low viscosity inks Lubricants e Paints e Low concentration Shampoo e High concentration polymer solutions Medium concen polymer solutions e Various solvents tration polymer solutions Appendix III Ordering VROC Chip and Syringe The VROC is offered in three chip specifications A B C to maximize the viscosity range for precise measurements The VROC B chip is our gen eral use viscometer The specialty VROC chips are as follows A chips Low viscosity Small sample volume A02 AO5 A10 0 2 cP 200 cP Low shear rate B chips Medium visc
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