Leica_Measuring_Manual_EN
Contents
1. wl 12mm 120 0 5 3 0 1 0 20 40 60 80 100 100 x 1 mm 5 100 50 mm ii ie 1 if eee eee eee ee cee ee Avoid corrosive cleaning agents and materials which will scratch the surfaces Observe the information relating to care in the operating manual for your instrument e Do notkeep unused graticules and stage micrometers in the original containers in which they were supplied You will not cause any damage by undertaking cleaning with e dry hairbrushes bellows optical cleaning cloths e cotton buds e fine soft non fluffing cloths if removing fingerprints dampen cloth if dealing with stubborn dirt soak cloth in alcohol Measuring User Manual 3 Setting diopters and grid plate Co ordinate the eyepieces precisely to your eyes as I described until you can clearly see the graticule and specimen at the same time free from parallax Preparations Setdistance from eye eyepieces illumination etc as described in the instrument s directions for use gt Set 0 diopters on both eyepieces gt Illuminate field of vision without specimen Use graticule to Do look into the eyepieces regulate eyepiece gt Turn eyepiece with graticule anticlockwise until it reaches the stop2. Look into the eyepieces gt Slowly turn eyepiece with graticule clockwise until you can clearly see the measurement lines e longer adjust eyepiece with grid plate 24 Focusing test Place test specimen e g stage specimen micrometer under the lens 4 Measuring User Manual Regulate eyepiece without graticule Checking eyepiece is free of parallax Do not look into the eyepieces gt gt Measuring User Manual Select the lowest magnification Observe specimen through the eyepiece with graticule and focus Select the highest magnification Use focusing drive to optimise sharpness Turn eyepiece without grid plate anticlockwise until it reaches the stop Select the lowest magnification Observe specimen through the eyepiece without graticule and slowly turn eyepiece clockwise until the eye clearly sees the specimen Select the highest y magnification Observe specimen through 8 both eyepieces If necessary use the focusing drive to re focus Look into the eyepieces and slightly move your head Graticule and specimen must not move towards one another i e they must be clearly focused in one plane free of parallax If this is not the case please repeat the process Calibrating graticules Why calibrate You do not have to calibrate You have to calibrate Only calibrate once Engaging zoom settings
3. 1 2 7 User Manual etca MICROSYSTEMS Accessories for measuring tasks Lengths Counting Calibrating Angle measurements Eyepieces Software for measuring tasks e Stage micrometer 1 for calibration e Graticules with various measuring pitches 2 in mm and inches e Graticule with mesh 3 e Graticule with co ordinate grid are measured using the graticules with measuring pitches e g 12mm 120 or 5mm 100 cells nuclei and the suchlike within one particular area can be undertaken using the grid plate with mesh When calibrating with the stage micrometer the value of an interval is defined on the eyepiece grid plate in relation to the specimen magnification can be taken with the aid of the graticule with co ordinate grid and the angular division on the rotary polarisation table Please refer to the instrument s directions for use with regard to using the graticule in eyepieces Two adjustable eyepieces are required for equipment with measuring graticules so that the sharpness can be set precisely If you take digital photographs with your stereo microscope or macroscope we would recommend the Leica IM image archiving and image management software with measuring module for interactive measuring inscribing and marking Measuring User Manual 0 1 2 3 4 5 6 7 8 9 12
4. The graticule is only magnified by the eyepiece The total magnification of the specimen does however depend onthe lens magnification changer eyepiece and additional tube possibly used e g coaxial lighting sources and changes whenever these factors are altered When calibrating with the stage micrometer the value of an interval on the graticule is defined in relation to the specimen magnification The true dimensions of a specimen can only be established once the calibrated value has been defined if you only undertake relative measurements or comparisons if you need absolute measurement values The calibration value of each of your optics magnification combinations used only has to be established once Keep a table of the appropriate calibration values refer to example on page 12 When working with instruments with engageable zoom settings Leica MZ6 MZ75 295 etc or adjustable stops Leica S6 models etc the specific magnifications can always be re produced exactly refer to directions for usage for the stereo microscope When using instruments with zoom magnification changers without engageable setting stages inaccuracies could arise when again undertaking settings If a high degree of measurement precision is required you should therefore undertake calibration whenever changing magnification Measuring User Manual Calibrating Calibration formula gt Place stage micrometer on the table i
5. e area may comprise several squares 8 Measuring User Manual Examples 1 Measuring pitch r 12mm 120 0 t 10 1 2 3 4 5 6 7 8 t x t Stage micrometer with 0 1mm Calibrating graduation 7 8mm the stage micrometer X 120 intervals on the eyepiece graticule Y mm 0 065 calibration value Measuring Intervals on the eyepiece graticule 112 Result 112 intervals 0 065mm 7 3mm length of measurement distance Measuring User Manual 2 Measuring pitch 5mm 100 Stage micrometer with 0 1mm graduation y 0 4 Calibrating 1 37mm on the stage micrometer X 100 intervals on the eyepiece graticule Y mm 0 014 calibration value a Measuring Intervals on the eyepiece graticule 34 Result 34 intervals 0 014mm 0 47mm length of measurement distance Measuring User Manual 3 Mesh pitch 100 x Imm Calibrating 6 2mm on the stage micrometer X 10 intervals on the eyepiece graticule Y 6 2 10 mm 0 62mm calibration value Counting Area of boxed in square 0 38 Result 4 cells per 0 3 8 Area of boxed in square 9 5mm Result 16 cells per 9 5mm Measuring User Manual 11 Calibrating 1 Focus stage micrometer 2 Count number of mm inches on the stage micrometer X number of intervals on the eyepiece gra
6. nsert and set sharpness gt Select the magnification with which you will later want to take measurements gt Set eyepiece graticule and se lens micrometer to be free of parallax see page 4 7 10123 4 5 6 7 8 1 x t gt Align stage micrometer X close to and in parallel with the eyepiece graticule Y gt Focus both pitches down to a single point In the example 0 and 10 gt Count The number of mm inches of the stage micrometer X corresponds to a certain number of intervals on the eyepiece graticule Y gt Inthe example 7 8mm on the stage micrometer corresponds to 120 intervals on the eyepiece graticule gt Calculate calibration value using calibration formula and note down X stage micrometer number of mm inches Y eyepiece graticule number of intervals EXS mm inch calibration value mm inch Y Measuring User Manual 7 asuring coun ung Measuring length gt Remove the stage micrometer and focus a specimen gt Count how many intervals on the eyepiece graticule cover the measurement distance required gt Multiply the number of intervals by the calibration value The result is the absolute length of the measurement distance in mm inch Counting en press REE P gt Remove the stage micrometer and focus on the specimen gt Count particles cells within a known area e Th
7. ticule 3 Calculate calibration value one interval of the eyepiece graticule mm inch calibration value Measuring 4 Focus on specimen rather than stage micrometer 5 Count and calculate number of intervals on the eyepiece graticule x mm inch calibration value mm inch length of measurement distance 2 gt 5 5 2 5 e 5 5 2 5 5 5 2 2 2 F 5 a 2 Leica Microsystems Switzerland Ltd Telephone 41 71 726 33 33 Stereo amp Macroscope Systems Fax 41 71 726 33 99 MICROSYSTEMS CH 9435 Heerbrugg www leica microsystems com www stereomicroscopy com Illustrations descriptions and technical data are not binding and may be changed without notice 2 275 0 Leica Microsystems Switzerland Ltd CH 9435 Heerbrugg 2001 Printed in Switzerland 1 2004 RDV
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