User Manual 751mT
Contents
1. Do not use this apparatus near water e To reduce the risk of fire or electric shock do not expose this apparatus to rain or moisture Objects filled with liquids should not be placed on the apparatus e Do not block any ventilation openings Install in accordance with the manufacturer s instructions Only use secure industry standard connectors and tubing for pressure connections Faults defects and mistakes due to wrong connections void warranty DMT is not responsible for results and mistakes caused by inappropriate pressure hookup e Do not install near any heat sources such as radiators heat registers stoves or other apparatus that produces heat e Only use attachments and accessories specified by the manufacturer e Unplug this apparatus during lightning storms or when unused for long periods of time The 751mT is delivered with an external 100 240VAC to 24VDC adapter Protect the power adapter and cord from being walked on or pinched Refer all servicing to qualified service personnel Servicing is required when the apparatus has been damaged in any way such as the power supply cord or plug being damaged liquid or objects having fallen into the apparatus exposures to rain or moisture abnormal operation or having been dropped SAFETY 5 CHAPTER 1 SYSTEM OVERVIEW 1 1 Mini Tissue Organ Bath System 751mT front panel Buffer 1 Buffer 2 e Isometric transducer a BE2. Rm 28C No 8 Dong Fang Road Lu Jia Zui Financial District Shanghai 200120 P R China TES O 21 25 1330 Fax 86 0 21 5877 0063 www dmt asia com salesQdmt asia com supportodmt asia com DMT USA Inc 201 East Liberty Street Suite 6 Ann Arbor MI 48104 USA Tel 1 770 612 8014 Fax 1 678 302 7013 www dmt usa com sales dmt usa com support dmt usa com
3. 6 1 2 Mini Tissue Organ Bath System 751mT rear panel iii T DAS E E Ew T 1 4 Different types of tissue sample NOICELS cccccseseseeeccsssssereneccccssseresececssuseresececceseeessceccccsneatsceccccseeesseecccusesesseececcseeersenceccessetes 8 Cha pir 2 dri t 9 EA A PP e e O E SEEK OUO AREE ERR NC 9 Chapter Key Function GeSCTriptiOn c sscccsssccsssseccnssecenssseensseeensseeensseeensseennaseoeassecensseennsseneaseeoneaseenassenenseeonsseneassenenssennsssenss 11 Chapter eg eet GS 13 4 1 Weight Calibration of hcic MU edu RT T M 13 2 2 The WES nt Calo ration Ol OC COUN drid ion 13 Chapter 5 Maintenant P OTT A G Ms 14 Appendix 1 System specifications aci 15 4 MINI TISSUE ORGAN BATH SYSTEM MODEL 751MT USER MANUAL SAFETY DMT Mini Tissue Organ Bath System 751mT has been designed for use only in teaching and research applications It is not in tended for clinical or critical life care use and should never be used for these purposes nor for the prevention diagnosis curing treatment or alleviation of disease injury or handicap e Do not open the unit the internal electronics pose a risk of electric shock
4. 751MT USER MANUAL CHAPTER 3 KEY FUNCTION DESCRIPTION DMT rowro Figure 3 1 Display Keyboard Select Temperature To display the Buffer Temperature press the Buffer Temp button The LED on the button lights and the heating plate temperature is displayed To change the Set Point Temperature for the buffer press the up or down Arrow Key After the Arrow Key is pressed the Set Point Temperature is displayed If the Arrow Keys are not touched for 7 seconds the display will return to the buffer temperature To display the Chamber Temperature press the Chamber Temp button The LED on the button lights and the Chamber Temperature is displayed To change the Chamber Set Point Temperature press the up or down Arrow Key After the Arrow Key is pressed the Chamber Set Point Temperature is displayed If the Arrow Keys are not touched for 7 seconds the display will return to the Chamber Temperature Arrow Buttons up and down Heat ON OFF This button is used for Chamber and Buffer Heat ON and OFF The first time the button is pressed the buffer heater 15 activated and the LED 15 flashing The second time the button is pressed the chamber heat is activated as well and the chamber light is turned On the LED is now lit constantly The third time the button is activated the heat and chamber light is turned Off Fill Buffer 1 Fillthe Chamber with buffer 1 Press the button and hold it down the buffer 1 filling pump is running as lon
5. DANISH MYO TECHNOL Ost MINI TISSUE ORGAN BATH SYSTEM MODEL 751 User Manual Version 1 1 2 MINI TISSUE ORGAN BATH SYSTEM MODEL 751 USER MANUAL MINI TISSUE ORGAN BATH SYSTEM MODEL 751MT TRADEMARKS PowerLab and LabChart are registered trademarks of ADInstruments Pty Ltd The names of specific recording units such as PowerLab 4 25 are trademarks of ADInstruments Pty Ltd DMT reserves the right to alter specifications as required This document was as far as possible accurate at the time of printing Changes may have been made to the software and hardware it describes since then New information may be supplied separately This documentation is provided with the Mini Tissue Organ Bath System Model 751mT All rights reserved No part of this manual may be reproduced or transmitted in any form or by any means without the written permission of Danish Myo Technology A S Every attempt is made to ensure accurate information misprints construction and specification changes can occur Danish Myo Technology A S reserves the right to alter change content as required and without any notice Copyright Danish Myo Technology A S DANISA MYO TECHAHNOCOG A TRADEMARKS 3 CONTENTS A 3 A E M V 5 Chapter i System OV A Wir rai Poca 6 1 1 Mini Tissue Organ Bath System 751mT front
6. NG UP 2 1 Mounting Set up Adjustment of the transducer and tissue holder position Figure 2 2 Transducer and tissue holder 1 Vertical fine adjustment of the transducer Turn the micrometer screw clockwise to lower the transducer Turn counter clockwise to lift the transducer The marked line is the lowest position of the transducer The highest position is 20 mm above the line 2 Horizontal adjustment of the transducer Turn the screw clockwise to free the transducer The transducer can now be moved horizontal Turn the screw counter clockwise to lock the transducer 3 Course adjustment of the lower tissue holder Turn the screw counter clockwise to unlock the tissue holder When unlocked it is possible to move the tissue holder up down left and right Turn screw clockwise to lock CHAPTER2 9 Tissue chamber handling The following is a description of how to remove place the tissue chamber 1 Move the transducer upwards Make sure the sample holder is free of the tissue chamber 2 Open the chamber door 3 Removing the glass chamber carefully pull the top of the chamber towards you a few millimetres until the chamber is free to move upwards Then lift out 4 Replacing the glass chamber place the stub at the bottom of the chamber inside the chamber disc Gently place the stub situated on the side of the glass chamber into the heating compartment Figure 2 3 Chamber handling 10 MINI TISSUE ORGAN BATH SYSTEM MODEL
7. T e Oxygenation carbogen regulator Chamber Qin Display Keyboard 9000000000000000000000000000000000000000000000090000000000000090000000000009000090 Figure 1 1 51mT front view 6 MINI TISSUE ORGAN BATH SYSTEM MODEL 751MT USER MANUAL 1 2 Mini Tissue Organ System 751mT rear panel Type OMT1090 Mini Tobs S N DMT1C30 100910 0001 Volt 24 Amp 58 ain Dre e ON OFF switch Grounding connect e data acquisition USB e Oxygenation carbogen inlet max 10bar 145psi Analogue recorder output e e Drainage connection for waste buffer USB connector Figure 1 2 751mT rear panel IMPORTANT TO GET A NOISE FREE RECORDED SIGNAL IT IS IMPORTANT TO CONNECT THE 751MT GROUND CONNECTION TO THE DATA ACQUISITION SYSTEM S GROUND CONNECTION 1 3 Chamber with tissue 20 ml chamber Drainage buffer overrun Tissue holder Tissue Temperature probe buffer inlet oxygenation carbogen gasses and drainage at the bottom Figure 1 3 Glass chamber 1 7 1 4 Different types of tissue sample holders Figure 1 4 Triangle hooks Figure 1 5 Closing clips Figure 1 6 Pins standard optional optional Figure 1 7 Triangle hooks w stimulation electrodes Figure 1 8 Triangle hooks w circular stimulation electrodes optional optional 8 MINI TISSUE ORGAN BATH SYSTEM MODEL 751MT USER MANUAL CHAPTER 2 SETTI
8. ansducer calibration is performed before first use the Tissue Organ Bath System The calibration is performed through the Display Keyboard using the appropriate weight according to gain setting As default the system is set with a weight range of 20g and the calibration is performed using a 5g weight See figure 4 1 above 4 2 The weight calibration procedure 1 Step 1 Remove weight from the transducer Press button FORCE ZERO and hold it down until the display shows CAL And the light diode in the button FORCE CAL lights up 2 Step 2 Put the 5g weight on the transducer and then press the FORCE CAL button The analog output is now calibrated to give bV out for 20g in the 20g range 5V out for 40g in the 40g range 5V out for 80g in the 80g range 2 5V out for 160g in the 160g range N N NN NOTE IF YOU WANT TO ABORT THE CALIBRATION ROUTINE BEFORE IT IS FINISHED JUST TURN OFF THE POWER As a part of the general maintenance of the Tissue Organ Bath System DMT recommends that the organ bath is weight calibrated at least once every month DMT also recommends that the bath is weight calibrated every time the system has been moved or has not been used for a long period of time CHAPTER 4 13 CHAPTER 5 MAINTENANCE DMT recommends that the following sections are read carefully and that the instructions are followed at all times Knob for dismounting chamberdisc and temperature probe Oxy
9. g as the button is pressed If the button is pressed for more than 60 seconds the pump stops and the button must be pressed again to resume filling Fill Buffer 2 Fillthe Chamber with buffer 2 Press the button and hold it down the buffer 2 filling pump is running as long as the button is pressed If the button is pressed for more than 60 seconds the pump stops and the button must be pressed again to resume filling CHAPTER 3 11 Empty chamber A short press on the button activates the empty valve for 13 seconds A new short press will deactivate the valve at any time Draining will continue while the button is pressed Zero the analog output Used to zero the analog output Force calibration Used to store a new weight calibration Force Range select When the button is pressed the range shifts to the next level The active range is indicated by the LED 40g 80g 160g Overload FORCE If the force input signal is out of range the light diode is lit green OVERLOAD 12 MINI TISSUE ORGAN BATH SYSTEM MODEL 751MT USER MANUAL 4 Figure 4 1 Transducer with calibration weight 4 1 Weight Calibration of the sensor Output Prior to shipment the 751mT has gone through two days of continuous testing including a final force transducer calibration However in order to ensure that the Tissue Organ Bath is working at highest performance DMT recommends that a new force tr
10. genation regulator Oxygenation tubing pipe Buffer 1 inlet to chamber e Buffer 1 inlet 9 Buffer 2 inlet to chamber e Buffer 2 inlet e Drainage tube pipe Temperature probe Figure 5 1 Tubes connected to the chamber Change the tubing after use of unknown or toxic chemicals or if any kind of hydrophobic reagent has been used The following tubes are changeable e Buffer 1 inlet e Buffer 2 inlet e Buffer inlet to chamber e Oxygenation tubing pipe e Drainage tube pipe IMPORTANT IT IS VERY IMPORTANT THAT THE TUBING USED IS THE ORIGINAL TUBING FROM DMT THE QUALITY DIAMETER AND LENGTH ARE SIGNIFICANT TO THE CHAMBER FILLING MECHANISM NOTE REGARDING THE THREE OXYGENATION REGULATORS THE REGULATORS NEED TO BE GREASED AT LEAST TWICE A YEAR ALSO ENSURE THAT THE REGULATORS ARE TURNED AT REGULAR INTERVALS TO PREVENT THEM FROM STICKING PLEASE USE DMT GREASE 14 MINI TISSUE ORGAN BATH SYSTEM MODEL 751MT USER MANUAL APPENDIX 1 SYSTEM SPECIFICATIONS Power supply Current max Dimensions LxDxH in cm Environmental humidity Environmental operating temp Environmental storage temp Chamber size Chamber material Chamber heating Chamber temperature range Chamber temp resolution Chamber temp stability Buffer bottle Buffer to chamber filling rate Buffer heating Buffer temperature range Buffer temp resolution Buffer temp stability NOTE 24VDC 1096 3 3Amp
11. s 45 x 26 x 30 20 to 80 RH non condensing 15 30 C 4 70 C 20ml Glass Heated air circulation 20 40 C Never below room temperature 0 1 C 0 2 C When target temperature attained 2 x litre Max 100ml min Heating plate 20 40 C Never below room temperature 0 1 C 3 0 C When target temperature attained TO MINIMIZE THE HEATING TIME ONE CAN BENEFIT BY PRE HEATING THE BUFFER TO 37 C BEFORE FILLING THE RESER VOIR BOTTLES FOR THE FIRST TIME IN THE DAY Net weight Analog output Analog output range Output impedance Air connection USB connector Data of Isometric transducer Max Range Operating temperature Frequency range 8 kg BNC connector at the back used to Connect to ext acquisition box Range 1 20g 2 5Volt Resolution O 01g Range 2 40g 2 5Volt Resolution 0 018 Range 3 80g 2 5Volt Resolution 0 018 Range 4 160g 2 5Volt Resolution 0 05g lt 200 Ohm For Download of Firmware 160gram 20 40 C 0 50 72 APPENDIX 1 15 DMT A S Skejby Science Center Skejbyparken 152 DK 8200 Aarhus N Tel 45 87 41 11 OO Fax 45 87 41 11 01 www dmt dk sales dmt dk support dmt dk DMT Asia Ltd Rm 2402B Great Eagle Centre 23 Harbour Road Wanchai Hong Kong S A R P R China Tel 852 6621 8337 Fax 852 3020 7554 www dmt asia com salesQdmt asia com supportedmt asia com DMT Asia China office
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