QX - 102 User Manual issue 3.1
Contents
1. To obtain best imaging conditions start with an acceleration voltage of 30 kV and a mid range spot size i e beam current corresponding to about 0 75 nA Incresse the contrast until the desired signal from the sample is obtained usually maximal contrast It is recommended to start imaging with a low scan speed a few seconds per frame N ote that since the signal from the sample is generally weaker and of lower contrast than the signal from the support grid the optimization of contrast and brightness should be carried out with respect to the sample and not to the grid Focus on the sample If you have difficulties to focus on your sample at this stage focus first on the supporting grid of the capsule using SE secondary electron detector then switch back to BSE detector The range of recommended acceleration voltage is 10 kV to 30 kV Different acceleration voltages correspond to different penetration depths Probe current spot size is determined empirically by the optimal signal obtained A higher probe current generates a larger signal affecting the resolution at higher magnifications A higher probe current may also damage the sample The optimal probe current spot size depends on the sample and is determined by finding a configuration which optimizes the signal and required resolution and minimizes the damage to the sample For maximal allowed probe current see Table 2 Scan speed should be adjusted according to the signal from the2. provide a convenient means to calibrate the parameters for optimal imaging conditions The Calibration Capsule is provided with an image to evaluate the optimization process Please read the instructions accompanying the product prior to use Follow the guidelines below to find the optimal imaging conditions Recommendations for parameters are summarized in Table 2 O ptimize the conditions first with the Calibration Capsule and then move on to imaging your sample m Placethedosed QX 102 capsule on the miaroscope stage as a conventional stub with the capsule membrane facing up In case the QX 102 capsule does not fit your SEM stage inquire for available adaptors m TheQX 102 capsules are suitable for use either with a high vacuum or alow vacuum mode m EnsuresuffiGent working distance N ote that the QX 102 capsules may be taller than conventional SEM stubs Lower the stage if necessary m Perform the imaging with a Back Scattered Electrons Detector BSED The capsule also enables X ray microanalysis using suitable detectors m Conventional Secondary Electron SE imaging can also be preformed but with lower signal For systems without a BSED consult QuantomiX representatives regarding the use of secondary electron detectors in conjunction with Q X 102 capsules m Forbestimagng adjust the working distance to maximize the sampleBSE signal See table 2 for recommended working distance range QX 102 User Manual Issue 3 1 26
3. 02 User Manual Issue 3 1 12 Cautions a Correct sealing of the capsule is essential for its proper functioning Capsule sealing is achieved when the wings of the liquid dish and the sealing stub are aligned b The QX 102 sealing stub includes a rubber seal If the rubber seal accidentally detaches from the sealing stub it should be re positioned with the flat surface away from the liquid dish as shown in Figure 3 c Do not use sharp objects such as sharp ended forceps to hold the rubber seal in order to prevent damage to the sealing d The QX 102 Capsules are intended for single use and are not reusable Figure3 Correct positioning of the Rubber Seal 13 Chapter 1 Introduction MP 10 Multi well Plate The MP 10 multi well plate is a sterile disposable plastic holder for the capsules designed to enable parallel handling of several QX 102 capsules It serves as a well plate for holding the capsules during various manipulations for culturing cells in QX 102 capsules and for inspection in an inverted light microscope The troughs along the sides of the multi well plate serve as water reservoirs for maintaining the humidity of the samples during incubations Sockets beside the reservoirs are designed for fitting a multi well aspirator into place i Note The MP 10 multi well plate is not compatible with autoclave sterilization Lid E a Water Sockets for Multi well Aspirator Base Figure 4 MP 10 Mult
4. 2 Using the QX 102 Capsules Chapter 2 Using the QX 102 Capsules The following protocols are common to all QX 102 applications m Opening the QX 102 capsules m Sampleinsertion and treatment m Preparation for imaging For detailed sample preparation instructions refer to the QX 102 Applications Manual Opening the QX 102 Capsules The QX 102 capsules and the MP 10 multi well plates are supplied sterile To maintain sterility open and handle the capsules in a sterile environment laminar flow l Caution Do not place the liquid dish or the capsule with the capsule membrane facing down except in the multi well plate To prevent rupture avoid touching the capsule membrane at all stages 135 Opening the QX 102 capsule 1 Inasterile environment cut the aluminum wrapping and peel back the adhesive paper from the pack of capsules Usethe transparent cover to maintain sterility after opening 2 Take the capsules out of the package one at a time by lifting up Please note that the capsules are supplied loosely closed To avoid accidental opening do not turn the sealing stub The capsules should stay closed at this point 3 Place the capsules one at a time into the MP 10 multi well plate Make sure that the capsule wings fit into the slits of the multi well plate as shown in Figure 6 a QX 102 User Manual Issue 3 0 18 4 Open the capsule by turning the sealing stub counter clockwise as shown in Figure 6 b 5 Store
5. Applications Manual or the website 2 Optional Exchange the liquid in the liquid dish to the QX Imaging Buffer as shown in Figure 9 a 3 Reassemble the capsule by attaching the sealing stub to the liquid dish and turning clockwise as shown in Figure 9 b till the wings are aligned and the sealing stub is closed on top of the liquid dish QX 102 User Manual Issue 3 1 24 Notes a If the rubber seal is not properly positioned on the sealing stub place it with the flat surface facing away from the liquid dish as shown in Figure 3 b The closed capsules can be stored at 4 C However the capsules should be equilibrated to room temperature before inserting them into the SEM 4 Place the QX 102 capsule in the SEM with the capsule membrane facing upwards as shown in Figure 9 c a Filling the Liquid Dishes with Capsule in the SEM Imaging Buffer c Placing the Figure 9 Preparing Samples for Imaging 25 Chapter 3 Imaging Chapter 3 Imaging Sample imaging in SEM using the WETSEM technology differs from standard SEM imaging in some aspects The factors that affect imaging vary among applications and different SEM types This chapter provides guidelines for achieving the best imaging results for wet samples with Q X technology The imaging conditions are best optimized using the Calibration Capsule cat no RT 56 The Calibration Capsule contains nanoparticles which are easily visualized in SEM and
6. UQX0001 Issue 3 1 November 2005 Q U A N TO M i TM ua QX 102 User Manual UQX001 Issue 3 1 O QuantomiX Ltd 2005 All rights reserved November 2005 QX 102 User Manual Issue 3 1 2 This publication is the copyright of QuantomiX Ltd and contains information that may not be used or reproduced unless agreed in writing QuantomiX Ltd reserves the right to alter without notice the specifications design or supply of any product or service The information provided in this User Manual is believed to be accurate It is the users responsibility to confirm the technical aspects and the suitability of the technology for any particular application For an overview of the QX 102 Capsule technology please view the Instruction CD supplied with the product QuantomiX Ltd 12 Hamada Street Tamar Science Park Rehovot POB 4037 Nes Ziona 70400 Israel Tel 4972 8 9462244 Fax 972 8 9465874 http www quantomix com 3 QX 102 User Manual Issue 3 1 Table of Contents List of Figures incitan na elven id 4 List of Tables 4 C ANREDE UNE 5 Technical Dita i a 6 QX 102 Capsules s O MP 10 Multi well plates 8 Chapter 1 Introduction sssssssssss emen 9 Manual Scope and Contents s 9 Rere ECES e en a a a a a a LO e a 10 Technical Support nenna 10 Technology and Components 10 TheQX 102 Capsule MP 10 Multi well Plate MA 4 Multi well Aspirator Calibration Capsule aaa LD QX 102 Im
7. aging Buffer e 16 Chapter 2 Using the QX 102 Capsules aaaaakakaakaka kaaa aaa 17 Opening the X102 Cal rar 17 Sample Application and Treatment aaa 19 Preparing the Samples for Imaging cinco 23 Chapter 3 Imaging eer reete ias 25 Appendix A Glossary sssssssssssss eI eee eren 29 Appendix B Troubleshooting 1 111418 31 Appendix C Ordering Information 33 Legal Notices ecc ati 35 Product Warranty Liability and License for USE 35 QX 102 User Manual Issue 3 1 List of Figures Figure Le Sealed Capsule ooo dni antro Figure 2 QX 102 Capsule Parts cc Figure 3 Correct positioning of the Rubber Seal Figure4 MP 10 Multi well Plate Figure5 MA 4 Multi well Aspirator a Figure6 Opening the Capsule Figure 7 Liquid Handling Figure8 Filling the Water Reservoirs for Incubation Figure 9 Preparing Samples for Imaging nnn List of Tables Tablel QX 102 Capsule Material Compatibility Table 2 Recommendations for SEM Imaging with Q X 102 Capsules 5 QX 102 User Manual Issue 3 1 Safety l Cautions a Correct sealing of the capsule is essential for its proper functioning Capsule sealing is achieved when the wings of the liquid dish and the sealing stub are aligned The QX 102 sealing stub includes a rubber seal If the rubber seal accidentally detaches from the sealing stub it should be re positi
8. apsules Notes a Do not leave the liquid dishes with low liquid levels for prolonged periods b Theaspirator is designed to leave 2 4 ul liquid in the dish after aspiration When changing solutions it is recommended to make additional washing steps with the next wash solution to ensure complete exchange c For more gentle aspiration lower the multi well aspirator gradually to its final position I Centrifuging the liquid dishes l Placethe multi well plate without the sealing stubs in a centrifuge holder suited for 96 well plates i Note For balance use a similar size plate such as a standard 24 well plate adjusted with water to the same weight 2 Apply a mild centrifugation for example 500 g for 5 minutes is usually sufficient IS Incubating the liquid dishes 1 Fill the elongated reservoirs along the edges of the MP 10 multi well plate base with 200 ul of distilled water each in order to maintain humidity during incubations see Figure 8 QX 102 User Manual Issue 3 1 22 Cautions a Be careful not to overfill and not to spill water outside the reservoirs b The base of the multi well plate must be dry while inserting it into the cell culture incubator If any liquid spills outside the reservoirs wipe it off carefully 2 Cover the MP 10 multi well plate with the lid to keep the capsules sterile and undamaged Note The MP 10 multi well plate can be used as a conventional multi well pl
9. ate such as 96 and 48 well plates with respect to applications involving incubators and light microscopy imaging Figure 8 Filling the Water Reservoirs for Incubation ES Storing the liquid dishes Ensure that each liquid dish contains 15 ul of water or buffer Fill the reservoirs of the MP 10 multi well plate with water Close the lid and store at 4 C Optional seal the closed MP 10 multi well plate with Parafilm Tet E 23 Chapter 2 Using the QX 102 Capsules Preparing the Samples for Imaging Liquid and other similar samples such as creams pastes and foams are imaged directly Samples that can be attached to the capsule membrane such as particles and fixed biological samples should be imaged in QX Imaging Buffer whenever applicable The QX Imaging Buffer is applied on the samples prior to imaging at the end of sample preparation steps Prolonged storage of the specimens in the imaging buffer is not recommended For imaging live biological specimens use the growth buffer of the samples instead of the Q X Imaging Buffer For detailed protocols for sample preparation for various applications please see the QX 102 Applications M anual For the latest applications protocols see our website www quantomix com I Caution Solutions containing DMSO are not suitable for imaging in QX 102 capsules IS Preparing the samples for SEM imaging 1 Apply and prepare the sample according to protocols provided in the QX 102
10. es representative or contact sales quantomix com QX 102 User Manual Issue 3 0 34 35 Legal Notices Legal Notices Product Warranty Liability and License for Use 1 QuantomiX guarantees the performance of all Products in the manner described in our product literature The Purchaser must determine the suitability of the product for its particular use or application Should any product fail to perform satisfactorily within a period of 12 months from receipt or within the shelf life expiry date of the product whichever is shorter due to any reason other than misuse or unsuitable application QuantomiX will replace it free of charge or refund the purchase price Please contact tech QuantomiX com or your local sales representative QuantomiX Q X capsules have been validated for single use in Scanning Electron Microscopes of the major manufacturers Reuse of capsules is prohibited is deemed misuse of the Products and releases QuantomiX from all warranty obligations QuantomiX products are covered by patents owned by QuantomiX Ltd as well as patents owned by Yeda Research and Development Co Ltd and licensed to QuantomiX Upon delivery of the Products QuantomiX shall be deemed to have granted the Purchaser a non transferable non exclusive license to usethe Products for the sole purpose of performing research and development applications The Purchaser will not have the right to market sub license or otherwise grant any rig
11. ht in or to the Products or make any commercial use or any disposition whatsoever in the Products Thelimited warranties set forth in these terms and conditions are given to the Purchaser only are not enforceable by any other entity or person and arethe sole and exclusive warranties given by QuantomiX with respect to the Products Quantomi X expressly disclaims any and all implied warranties including but not limited to implied warranties of merchantability and fitness for a particular purpose title and non infringement In no event shall QuantomiX bear any liability obligation or responsibility for any indirect incidental or consequential damages in connection with the Products regardless of the form of action including but not limited to loss of revenue or anticipated profits arising in any way in connection with the use of the Products In no event shall QuantomiX be liable for any amount greater than the amount paid to it in respect to the specific product giving rise to the liability QuantomiX Ltd 12 Hamada Street Tamar Science Park Rehovot POB 4037 Nes Ziona 70400 Israel Tel 4972 8 9462244 Fax 4972 8 9465874 QuantomiX Ltd 12 Hamada Street Tamar Science Park Rehovot POB 4037 Nes Ziona 70400 Israel Tel 972 8 9462244 Fax 972 8 9465874
12. i well Plate QX 102 User Manual Issue 3 1 14 MA 4 Multi well Aspirator TheMA 4 multi well aspirator system is designed to safely aspirate liquids from the QX 102 capsules and is required for applications that need liquid exchange in capsules Other means of aspirating liquids from the liquid dish should be avoided as they may lead to capsule membrane rupture The multi well aspirator drains liquids simultaneously from up to four capsules placed in a row in the MP 10 multi well plate The legs on both ends of the multi well aspirator fit into the sockets of the MP 10 multi well plate Inserting the legs into the sockets will place the four suction pins of the multi well aspirator into the liquid dishes of the capsules placed in a row in a multi well plate Please note that a small volume of liquid will always be left in the liquid dish The MA 4 multi well aspirator is designed to leave between 2 ul to 4 ul of liquid in the dish after aspiration to prevent sample drying A vacuum control valve serves for adjusting the aspiration rate see Figure 5 Slow suction is recommended to prevent dislocation of loosely attached cells For detailed instructions refer to the MA 4 User Guide from aspirator accompanying the product A o A sa vacuum to trap y a control vacuum bottle source Figure 5 MA 4 Multi well Aspirator 15 Chapter 1 Introduction Calibration Capsule The Calibration Capsule cat no RT 56
13. is a QX capsule designed for finding the optimal imaging conditions for wet samples with W ETSEM technology i Note We strongly recommend new users to initially use the Calibration Capsule to find the optimal working conditions in the SEM Imaging of wet sample with QX 102 Capsule in a SEM differs from standard SEM imaging in some aspects The factors that affect imaging vary among applications and differ from one SEM model to another The Calibration Capsule contains nanoparticles 40 and 500 nm in size stably attached to the capsule membrane The particles are easily imaged in a SEM and provide a convenient means to calibrate the parameters for optimal wet imaging conditions Note Always use the Calibration Capsule with QX Imaging Buffer For details of use and calibration please refer to the instruction page accompanying the product QX 102 User Manual Issue 3 1 16 QX Imaging Buffer QX Imaging Buffer cat no 1B 64 is a solution optimized for imaging samples in a SEM with QX 102 capsules and is formulated to minimize damage to the samples by the electron beam Imaging of samples should be done in QX Imaging Buffer whenever applicable and it is especially recommended for biological samples QX Imaging Buffer is applied on samples after sample preparation steps prior to imaging as described in Preparing Samples for Imaging For product details please refer to instructions accompanying the product 17 Chapter
14. ls do The attachment Use other attachment factors Growth not attach to factor in use does the capsule not support cell membrane growth QX 102 User Manual Issue 2 0 32 The cells do not grow well The growth conditions are not optimal Some cells may require specific conditions for growth in a QX 102 capsule Adjust the density of the cells or the incubation period Traces of toxic materials spilled onto the MP 10 multi well plate affect the cell growth An MP 10 multi well plate that has been used for staining with toxic reagents should not be used for cell growth Cell Staining Imaging The cells look damaged after the staining Samples have dried while being handled Do not leave liquid dishes with low liquid levels for prolonged periods of time labeling procedures No signal is The sample is For protocols of sample observed not in contact attachment see Chapter 3 and with the capsule Chapter 4 of the QX 102 membrane Applications Manual The image is There is no The sample may require not clear sufficient contrast enhancement such as contrast between constituents of the sample heavy metal staining For staining of biological samples see Chapter 4 of the QX 102 Applications Manual 33 Appendix C Ordering Information Appendix C Ordering Information Please see www quantomix com for your local distributor or Quantomix sal
15. o light microscopy The Q X 102 capsules can be used for imaging various wet materials and biological samples including liquid samples foods cosmetics oils paints drugs etc particles in solutions adherent and non adherent cultured cells and microorganisms The samples can be visualized either directly or by following the appropriate contrast enhancement staining or labeling procedures for which protocols are provided in the QX 102 Applications M anual The QuantomiX components required for using the technology are QX 102 capsules MP 10 multi well plate MA 4 multi well aspirator Calibration Capsule QX Imaging Buffer 11 Chapter 1 Introduction The QX 702 Capsule The QX 102 capsule shown in Figure 2 is a sterile single use specimen enclosure consisting of a liquid dish and a sealing stub The liquid dish is the base of the capsule which is designed as a cell culture dish for the growth of adherent cells attachment of non adherent cells and convenient deposition of other liquid samples The sealing stub should be engaged in order to seal the capsule using a two step closure action m Turningto the first click attaches the sealing stub loosely to the liquid dish m Turning to the second click closes it tightly and seals the capsule Figure 2 QX 102 Capsule Parts 1 Sealing stub includes a rubber seal Membrane Ss 2 Liquid dish includes a Rubber Seal membrane with supporting grid T je QX 1
16. oned with the flat surface away from the liquid dish Use powder free gloves to maintain cleanness and sterility of the QX 102 capsules Powdered gloves should be avoided To prevent rupture avoid touching the capsule membrane at all stages Do not place the liquid dish or the capsule with the capsule membrane facing down except in the multi well plate QX 102 User Manual Issue 3 1 Technical Data QX 102 Capsules Storage Shelf Life Application Dimensions Sterility Should bestored in a dry dark environment at room temperature 18 months from specified production date The capsules are intended for single use and are not reusable The products are intended for research purposes only Suitable for liquid samples and particles that can be adhered to the capsule membrane including cultured cells and micro organisms Not suitable for large thick samples such as tissues plants for these samples inquire about other capsule types at tech quantomix com Liquid dish diameter 3 mm working volume 15 ul Sealed capsule see Figure 1 Dimensions in mm Figure 1 Sealed Capsule In cases where the Q X 102 capsule does not fit your SEM stage inquire for available adaptors Gamma sterilized Opening of the packaging should be done in a sterile environment laminar flow Opened packages should be stored under sterile conditions 7 QX 102 User Manual Issue 3 1 Operation 4 to 40 C Temperature Centrif
17. rainage system designed to safely aspirate liquids from the Q X 102 capsules without damaging the capsule s membrane Capsule used for SEM imaging of various wet materials and biological samples Q X 102 capsule base designed as a miniature cell culture dish for applying samples Part of the Q X 102 capsule used for sealing the capsule and for holding the capsule in the SEM Q X capsule with control sample used for optimization of imaging conditions Buffer optimized for imaging samples in SEM with Q X 102 capsules Back scattered electrons detector Back scattered electrons Secondary electrons QX 102 User Manual Issue 3 1 30 31 Appendix B Troubleshooting Appendix B Troubleshooting Phase Problem Possible Cause Solution Liquid Liquid is The capsule a Avoid touching the capsule Handling leaking out of membrane has membrane at any time the liquid dish been damaged b Always place the capsules in the MP 10 multi well plate c Do not use means other than the MA 4 multi well aspirator for aspirating liquids Sample Vacuum used for Use weaker vacuum for liquid particles cells liquid handling is handling are detached too strong during liquid See Instructions for MA 4 handling especially from Sample particles Increase the concentration of thecenterot cells are not the attachment factor or try the liquid dish attached well other factors Cell The cel
18. sample For low contrast samples it is recommended to work with lower scan speeds For samples sensitive to higher beam damage it is recommended to integrate several frames instead of scanning one frame at a lower scan speed 27 Chapter 3 Imaging QX 102 Capsules Table 2 Recommendations for SEM Imaging with Parameter Recommended Comments range Acceleration voltage 15 30 kV Not lower than 10 kV Probe current based on source type Tungsten filament 0 4 1 0 nA Not higher than 1 0 nA FEG 0 1 0 5 nA Not higher than 0 5 nA Working distance based on detector type Semiconductor BSE 6 10 mm Acceptable 5 15 mm Robinson BSE 10 20 mm Better efficiency at higher kV Scintillator BSE 8 12 mm Acceptable 6 10 mm Everhart Thornley SE Determined Acceptable 6 15 mm empirically In lens Through the 2 4 mm Manufacturer dependent lens all detectors QX 102 User Manual Issue 3 1 28 29 Appendix A Glossary Appendix A Glossary Term SEM MP 10 MA 4 QX 102 Liquid Dish Sealing Stub Calibration Capsule QX Imaging Buffer BSED BSE SE Description Scanning electron microscope Multi well plate a sterile transparent holder for parallel handling of up to 24 individual Q X 102 capsules serving as a cell culture apparatus and holding the capsules during various manipulations Multi well aspirator a parallel d
19. the sealing stub separately until the samples are ready for imaging the stubs are most conveniently stored in the original packaging see Figure 6 c 6 Proceed to sample preparation a Placing the Capsule b Opening the Capsule c Placing the Sealing Stub Figure 6 Opening the Capsule 19 Chapter 2 Using the QX 102 Capsules Sample Application and Treatment Sample introduction and treatment consists mainly of adding liquids into the dish in many cases liquid removal and replacement incubations for various times and centrifugation of the capsules are also required While liquid addition is done using standard pipetting devices liquid removal should ONLY be done using the MA 4 multi well aspirator To ensure optimal results read and follow the detailed instructions provided below Setting up the workspace Applying liquids to the liquid dishes Removing liquids from the liquid dishes Centrifuging the liquid dishes Incubating the liquid dishes Storing the liquid dishes Setting up the workspace dj The work should be done on alab bench in a chemical fume hood or in asterile laminar flow hood according to the nature of the sample and the process 2 If liquids are to be removed from the capsules set up a vacuum source and connect the M A 4 multi well aspirator according to the instructions accompanying the product 3 A pipetting device suitable for 15 ul is required when treating multiple samples a repeti
20. tive dispensing pipette is most convenient 13 Applying liquids to the liquid dishes l Perform all pipetting and other manipulations with the liquid dishes properly positioned in the multi well plate 2 Apply liquids and sample suspensions into the liquid dish using standard lab pipettes The recommended working volume is 15 ul per liquid dish QX 102 User Manual Issue 3 1 20 Cautions a Do not touch the capsule membrane with the pipette tips Apply the liquid carefully at the edge of the liquid dish as shown in Figure 7 a b Do not overfill Excess liquid may spill over and wet the external side of the capsule membrane which may leave residues such as dried salts that interfere with SEM imaging c Volumes smaller than 15 pl can also be used However note that smaller liquid volumes evaporate easily and can cause drying of the samples a Applying Liquids b Removing Liquids Figure 7 Liquid Handling JS Removing liquids from the liquid dishes 1 Removing liquids from the liquid dish is done using the MA 4 multi well aspirator 2 Insert the legs of the MA 4 multi well aspirator into the sockets of the MP 10 multi well plate in order to place the suction pins into the liquid dishes that are positioned in a row in the multi well plate as shown in Figure 7 b 3 For best results aspirate the liquid from one row and replenish with the next wash solution immediately 21 Chapter 2 Using the QX 102 C
21. tocols for preparing the samples are provided in the QX 102 Applications Manual For the latest applications protocols see our website www quantomix com The User Manual consists of the following chapters and appendices Chapter Appendix Heading 1 Introduction 2 Using the QX 102 Capsules 3 Imaging A Glossary B Troubleshooting C Ordering information Legal N otices Provides The manual scope and contents and a detailed description of QX 102 capsules and accessories The general procedures for handling QX 102 capsules and preparing samples for imaging Guidelines for effective SEM imaging using the QX capsules The terms and abbreviations of the User Manual Tips for problem solving Contact information for the purchase of the Q X capsules Product Warranty Liability and License for Use QX 102 User Manual Issue 3 1 10 References Sites http www quantomix com Technical Support For technical support please contact tech quantomix com Technology and Components The WETSEM technology is a proprietary technology developed by QuantomiX Ltd which enables direct imaging of fully hydrated samples in scanning electron microscopes The patented technology is based on a thin electron transparent membrane which seals the sample from the vacuum in the microscope chamber N o coating or embedding of the sample are required enabling electron microscopy imaging with easy sample preparation comparable t
22. ugation Can be centrifuged at up to 2 500g Material For compatibility of reagents with the Q X 102 Capsule Compatibility materials see Table 1 below For other materials or specific concentrations please contact QuantomiX representatives at tech quantomix com For materials not compatible with QX 102 capsule inquire about other capsule types Reagents containing DMSO should not be used during SEM imaging DMSO can be present in the sample preparation process Table 1 QX 102 Capsule Material Compatibility Reagent Compatible NOT Compatible Acetone X Ethanol X Ethyl acetate X DMSO X 2 Glutaraldehyde Isopropanol M ethanol 4 Paraformaldehyde 1 Tannic acid 0 5 Triton amp X 100 0 5 Tween amp 20 Toluene X Xylene X X X X OX KK X QX 102 User Manual Issue 3 1 MP 10 Multi well plates Storage Should be stored in a dry dark environment at room temperature Shelf Life Three years from specified production date Application The products are intended for single use and are not reusable Suitable for cell culture conditions Dimensions 85 x 128 x 12 2 mm Sterility ETO sterilized Operation 4 to 40 C Temperature Centrifugation Can be centrifuged at up to 2 500g Chapter 1 Introduction Chapter 1 Introduction Manual Scope and Contents This manual provides a detailed description of the components required for using the Q X 102 capsule and guidelines for applying samples and imaging Specific pro
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