Agar nutrient in accordance with DEV
Contents
1. User manual DEV Nutrient Agar Product No 2 04726 782 page 1 2 1 Information Nutrient medium for the detection of Total Viable Count for water as regulated per the German standard method DEV and drinking water regulations or for various food products and rinse water samples DEV Agar pH 7 2 0 2 is a ready to use agar for a fast and safe detection of the total amount of colonies in drinking mineral and table water in various food and rinse water samples It can be used in the pour and spread plate method or as a basic culture medium for membrane filtration 2 Handling Required Material Water bath max temp 95 C 203 F Microbiological workbench Sterile Petri dishes Inoculation loop Drigalski spatula or sample swabs if necessary 2 incubators Application Handle the samples under sterile conditions to avoid secondary contamination Liquefy the nutrient media in a water bath at max 95 C 203 F approx 1 h Cool it for use to approx 48 50 C 118 122 F For the pour plate method as indicated by the DEV Place 1 mL of the test sample in a 9 cm Petri dish and fill up with the fluid and warm agar to a height of 3 4 mm Swirl the mixture gently by moving the closed Petri dish in a rotation following a 8 figure on the workbench As a Nutrient base Fill the fluid and warm culture media into a Petri dish to a height of 3 4 mm Cool the dish until the DEV Agar solidifies and use it for e membrane f2. a temperature of 121 C 250 F for 20 min Warnings Do not cook or freeze the culture media Do not keep the agar liquified longer than 4 h Media not directly used should not be solidified again for future use Repeated heating can restrain its functionality Always wear protective clothing when handling hot media 4 Related products Format Description ELLET a D ce Item no Investigation Relevant target Produce material microorganisms Nutrient Compex agar Total viable count Agar in Application Basic culture medium 9 x 250 mL Water TVC accordance for membrane filtration pour plate glass bottle AROF AI sr oes with DEV and spread plate method Drinking water Spring water 7 2 Table and mineral water Water specific faecal Well water indicator c i ATA f oncentrated Selective bouillon as qualitative 9x50 mL Rinse water Edo kaolo broth pre test glass bottle 37 2 04713 700 bacteria Version 4 0 19 June 2015 this version replaces all previous versions
3. ilters by placing the filter on top of the agar e for microbial spreading with a inoculation loop Drigalski spatula or sample swabs Incubation For water analyses according to DEV Place one test sample into an incubator at 35 39 C 95 102 F and incubate for 1 3 d Incubate the other test sample at 20 C 68 F for 48 hours Version 4 0 19 June 2015 this version replaces all previous versions User manual DEV Nutrient Agar Product No 2 04726 782 page 2 2 Evaluation After the appropriate period of incubation count the colonies on the nutrient media Colony Forming Units CFU If the number of CFU cannot be counted due a high germ density testing must be repeated using a dilution of the sample Water samples should not exceed 20 CFU in an incubation period of 1 3 d at 35 39 C 95 102 F and 100 CFU in 48 h at 20 C 68 F 3 Storage and Packaging Information Packaging and Content unit Cardboard Box 9x250 mL in glass bottles unit size Box approx 22 cm x 22 cm x 18 cm 8 7 in x 8 7 in x 7 1 in unit gross weight Box approx 4 1 kg 9 lbs Storage Store at 4 8 C 40 46 F according to product specification Store under dry and dark conditions Do not freeze product Waste Disposal No dangerous good No hazardous material Please consider your local waste regulations Not inoculated agar can be disposed of with normal laboratory waste Inoculated and incubated samples are to be sterilized before disposal at
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