ERP-27 User Manual
Contents
1. Panel Ground These banana jack outputs allow you to reference to the ERP 27 s internal ground The PGnd signal is generally used for signal referencing and connected to the E panel reference input The other two banana jacks provide a convenient connection for anti static wrist straps when working with any static sensitive electronics or directly to test subjects to avoid static discharge to exposed electrode connections NOTE These connections should not be used as a current sink Current running into these connections may cause unwanted voltage offsets for other signals on the ERP 27 Power Control Turns the power on the ERP 27 off and on Neuralynx Inc ERP 27 User Manual Page 6 Rev 1 21 Last Revised April 20 2009 Panel Status Indicators These LEDs provide information about the current status of the ERP 27 The indicator LEDs are defined below Table 2 Indicator LEDs Indicator Label GREEN RED V 5V Power on the ERP 27 is on y 5V Power on the ERP 27 is on Normal current flow in 5 V l Over current detected in I power supply when ERP 27 is An the 5V power supply pone eee a Y Over current detected in l power supply when ERP 27 is On the 5V power supply Fault upper Fuse for the 5V power PP supply is blown or not present Fuse for the 5V power supply is blown or not present Fault lower OFF 5V Power on the ERP 27 is off 5V Power on the ERP2. CSC8 2 JAMP4 2 56 63 7 CSC1 CSC8 1 JAMP4 1 48 55 6 B5 B12 2 JAMP3 2 40 47 5 AQ B4 2 JAMP2 2 32 39 4 A1 A8 2 JAMP1 2 24 31 3 B5 B12 1 JAMP3 1 16 23 2 AQ B4 1 JAMP2 1 8 15 1 A1 A8 1 JAMP1 1 0 7 14 Appendix A An Example using setup from Figure 10 1 For this example we have a system with an ERP 27 but only a single amplifier Let us assume that you have a single electrode in the brain whose wire connects to A1 on the headstage The A1 signal on the headstage will connect to the A1 channel on the ERP Now you would like to see the A1 signal show up as both a spike channel and also a continuous sampling so you can monitor the signal as an EEG inside of Cheetah Since the ERP automatically maps Al to the JAMP1 connector on the back of the ERP this only gives you one way to get the signal A1 out of the ERP I digress from our example for a quick look at the ERP s CSC connections If you look at any headstage you will notice that it does not contain any channels labeled CSC this is because the CSC channels of the ERP are designed to be able to input any external signal into Cheetah If there is nothing plugged into the banana connectors on the front of the ERP there will be NO signals on any of the CSC channels leaving the ERP ERP channels CSC1 CSC8 always map to the JAMP4 connector on the back of the ERP Back to the example So you may ask if I m interested in the signal on Al
3. Neuralynx Inc ERP 27 User Manual Page 14 Rev 1 21 Last Revised April 20 2009 Step 2 ERP AICA Cable Connection On the back of the ERP are four male 20 pin connections connect the ERP AICA cable the end labeled J AMP to these connections Connection 1 will be the TOPMOST of these male connections The other end of the ERP AICA cable labeled AMP should be connected to the corresponding input labeled nput of the Lynx 8 amplifier number the bottom most amplifier is number 1 nx 8 Neuralynx_ Amplifier Neural Yeer Neuralynx MPDOIl MX O Neuralynx Amplifier Lynx 8 Neuralynx Amplifier Figure 6 4 L8 Amplifier connections For systems without four L8 amplifiers per ERP 27 remember that the signals being output through the JAMPI JAMP4 connectors are hard wired and cannot be changed Use the descriptions of the Headstage Signal Outputs and CSC Signal Outputs from Neuralynx Inc ERP 27 User Manual Page 15 Rev 1 21 Last Revised April 20 2009 Step 3 Headstage Tether Connection The ERP 27 can be used with a variety of headstages offered by Neuralynx For the purpose of this setup we are using a HS 27 Other headstages will mount similar to this headstage if you have any problems connecting your specific headstage please see the documentation provided with your headstage or contact Neuralynx Using a headstage to tether adapter Figure 6 5 Tether Adapter Micro Connect the headstage s tethe
4. the electrode signals to be conveniently recorded in this manner more on this later Neuralynx Inc ERP 27 User Manual Page 4 Rev 1 21 Last Revised April 20 2009 3 Front Panel Panel Reference Inputs rf Continuously Sampled Channel Amplifier Connections CSC Signal ERP 27 a g 3 CSC EEG Reference Patch Panel for klari HS 27 Series Headstage Preamp Boards and Lynx 8 Amplifier Systems Headstage Signal Outputs CSC Reference Selectors External in Reference Inputs Headstage Signal Reference Selectors Power Control HS Power Control Neuralynx Inc Tucson AZ USA Headstage Signals Extra Headstage Panel Ground Panel Status Signal Outputs Indicators Figure 3 1 ERP 27 Front Panel Overview Headstage Signal Outputs These banana jacks provide an output for the unity gain buffered signals from the headstage In addition to outputting the headstage signal through these banana jacks the ERP 27 is hard wired to send the signal to the L8 amplifiers Using these outputs does not affect the signal being sent to the amplifiers These connections are most commonly used for monitoring headstage signals on an external device i e oscilloscope or for connecting to one of the signal inputs on the ERP 27 The labeling on the ERP is designed to mimic the labels on the electrode interface board EIB used with the HS 27 The signal name can be determined by taking the letter to the left of the
5. 27 is off 5V Power on the ERP 27 is off 5V Power on the ERP 27 is off 5V fuse is in tact and present 5V fuse is in tact and present The I and I indicators may normally glow dimly even when an HS 27 is not connected to the ERP 27 J L V and V will turn off when a fault is detected Neuralynx Inc ERP 27 User Manual Rev 1 21 Last Revised April 20 2009 Page 7 CSC Signal Inputs These banana jacks allow you to input any signal max 20V min 20V into the system Signals connected to these inputs will be treated as if they had come directly from the headstage This will be useful for recording signals that do not come from the headstage i e a stimulus signal or allow you to record one of the headstage signals with different amplifier settings One misconception is that these inputs are related to the continuously sampled channels CSC in the Cheetah software this is not so The CSC inputs on the ERP 27 are signal inputs only they are treated identically to the regular headstage signals in the Cheetah software CSC Reference Selectors The reference selectors for the CSC inputs are identical to the headstage reference selectors with one exception they have a sixth position for an external reference signal This external signal is obtained from the External Reference Input directly beneath the selector switch External Reference Inputs The external reference inputs fu
6. 42 support neuralynx com Neuralynx Inc ERP 27 User Manual Page 3 Rev 1 21 Last Revised April 20 2009 2 ERP 27 Overview The ERP 27 provides the central connection point and controls for The electrode headstage preamp Four Neuralynx Lynx 8 Amplifiers Eight external user input signals with reference inputs Five configurable global references Two bipolar external Stimulus Isolation Units SIU The ERP 27 provides an intuitive and flexible user controlled selection of reference sources and reference selection for system amplifier inputs This device is designed to interface to the Neuralynx HS 27 series of headstage preamps The 27 channels on the HS 27 are active buffered electrode input channels which are commonly classified as 24 input electrodes with two extra EEG channels and one reference electrode channel The ERP 27 interfaces to four Neuralynx Lynx 8 L8 amplifiers 32 channels total The 24 single unit electrode signals are routed directly to the 24 signal inputs of the first three amplifiers The fourth L8 amplifier is connected to the Continuously Sampled Channel connection area on the ERP 27 This allows a very convenient method for amplifying and filtering the same electrode signal with different gains and filter settings for example to record both single unit activity high frequency bandpass and large amplification and EEG low frequency bandpass and lower amplification from one electrode This allows for eight of
7. MDR style connector The tether is connected to this connector through a tether adapter sold separately that will convert the Micro DB37 tether connection to the 3M MDR connection This connection should always be used with a Neuralynx supplied connector the pin breakout is provided for informational purposes only Yia stloo PO as alol XIX a xjx x xxx 1 Hig x is D wig ou a STS SSH e HOON OW TON e 5 50 4 43 2 1 0 9 8 7 6 5 34 33 4 1 Reference 27 Power_Neg 26 PGnd Gnd Ref Power Pos Power _Neg Y3 PGnd Tether Shield PGnd Figure 4 4 Headstage Tether Connection Pin Breakout All the descriptions of the labels are discussed elsewhere in this document The X and Y labels correspond to A and B headstage signals respectively Neuralynx Inc ERP 27 User Manual Page 11 Rev 1 21 Last Revised April 20 2009 Headstage Signal Outputs These three 20 pin connectors are labeled JAMP1 JAMP2 and JAMP3 from top to bottom These connectors output both the headstage signal and its selected reference signal The headstage signal outputs are designed to connect directly to the front Input connector of a L8 amplifier The headstage signal outputs are hard wired and cannot be changed They are mapped as follows Connector Headstage
8. Signals JAMP1 A1 gt A8 JAMP2 A9 gt A12 B1 gt B4 JAMP3 B5 gt B12 CSC Signal Outputs This 20 pin connector labeled as JAMP4 is dedicated to the eight CSC inputs on the front of the ERP 27 This connector outputs both the CSC input signals and their selected reference signal This output is designed to connect directly to the front Input connector of a L8 amplifier The signals on this output are hard wired and cannot be changed This chapter will take you through the setup process for the ERP 27 Since the ERP 27 relies on other Neuralynx products to operate correctly you may see references to other products that you might not necessarily possess This guide will reference the most common components used with the ERP 27 if your components differ from those mentioned please see the documentation for your specific products If you have questions about obtaining any of the products mentioned in this section please contact Neuralynx for more information 5 Mounting The ERP 27 is designed to fit into a standard 19 rack mount system It is assumed that you have purchased an appropriate rack and mounting hardware separately It is highly recommended that the ERP 27 be rack mounted Use of nylon washers between the mounting screws and the Neuralynx equipment faceplate will prevent scratching of the faceplate paint If you do not have this equipment please contact Neuralynx for information on obtaining rack mount hardware Fi
9. an external signal and reference Connecting the external signal to the CSC input and the external reference under CSC as shown above will allow your external signal to be filtered by the L8 amplifiers and make the signal visible in the Cheetah software You need to make sure the reference selector switch is in the bottom position in order to reference to the external reference signal Neuralynx Inc ERP 27 User Manual Page 21 Rev 1 21 Last Revised April 20 2009 13 Amplifier and Analog to Digital Channel Mapping When configuring Cheetah it is very important to know how the electrodes in the brain relate to the ERP channels and the computer s A D channels On all headstages there are labels corresponding to the ERP channels i e Al on the headstage maps to A1 on the ERP The confusion comes when moving from the ERP channels to the A D channels The most important thing to remember is that the A D channels are a property of the amplifier NOT the ERP When connecting the Cheetah 32 Analog Cable to an amplifier you are setting the A D channel for that amplifier When setting up the amplifiers as described in this guide you will always have channels 0 7 on the bottom amplifier 8 15 on the 2 to bottom etc No matter what connection your amplifier has to the ERP the A D channel values will not change The mapping between the A D channels and your displays can be found in your electrode setup file The electrode setup file that is cu
10. ave connected your amplifier as described in this manual the first two channels leaving your amplifier will be A D channels 0 and 1 which just happens to now contain CSC1 and CSC2 respectively Now it is just a matter of configuring your electrode setup file to use those signals appropriately Which if you don t know how to do I suggest you take a look at some electrode configuration files and read the Setup File Command Reference help file under Cheetah s help menu The main point of this example is that you should not associate what is written on the front of the ERP with what is shown in Cheetah Just because it says CSC on the ERP does not mean it will be a CSC inside of Cheetah Neuralynx Inc ERP 27 User Manual Page 24 Rev 1 21 Last Revised April 20 2009
11. e no activity you might want to try using this channel as reference Below is a typical deviation from the standard reference setup In this example A is the quiet channel that we wish all other channels to reference The lines indicate banana plug connections the dots indicate switch position Only the A signals from the headstage are shown with the appropriate reference selection Neuralynx Inc ERP 27 User Manual Page 18 Rev 1 21 Last Revised April 20 2009 Headstage Electrode Signals Continuously Sampled Channel Amplifier Connections 2990990999909 ERP 27 9999995 g CSC f EEG Reference Patch Panel 5 for Neuralynx HS 27 Series Headstage Preamp Boards a ET SY 1 P E 1 mem r pea y p a oa e A e a a and Lynx 8 Amplifier Systems E xter nal i Reference B nce Connections s 2 3 4 5 6 7 8 9 10 11 12 A bc D Wt Falo a E E T E E T T E SS Fl E2 rol PGad Panel Ground VY 1 Fault Off Neuralynx Inc Tucson AZ USA Headstage Signals HS Power Control Figure 10 1 Using a quiet channel as reference In this configuration all of the A signals from the headstage will be referenced to the signal present on A when sent to the L8 amplifiers The output of the L8 amplifiers will be the headstage signal minus A amplified This means that there will be no amplified Al signal as it is referenced to itself Neuralynx Inc ERP 27 User Manual Page 19 Rev 1 21 Last Revised April 20 2009 11 Us
12. eserssresstenrestensessresresseeseseeesee 18 Figure 10 1 Using a quiet channel as reference seseeeeseeeseesessersresressessresreesrrseesrresee 19 Figure 11 1 Using a signal multiple times eeseeeeeseseseeseesesesssressessrerressessresreeseeseeseresee 20 Figure 12 1 Inputting an external signal and reference esessseeereerserersrrsresrresresseseresee 21 Table 1 Signal Description Sisseneri a e e a eE E Ea Ea i E 6 Table 2 ANIC ator LEDS meei nonante a a a aa 7 Table 3 Stimulus Breakout Table sa 225 tate ee ts Rees 10 Table 4 HS Stim Plug Requirements 5 2 02 uiiec scat nenaientinenaiotaiees 11 Table SERR Rick Mounted vrro nye inea aA tat a gis A A S 13 TableG Cheetah Oyrarnar a a AA E O a 22 Table 7 Cheetah 32nn en A a a E A A RE 22 Table Cheetah kc i cw a a a aac cee ue ese 23 Neuralynx Inc ERP 27 User Manual Page 2 Rev 1 21 Last Revised April 20 2009 1 Document Overview This manual is intended as an overview of the 27 channel Electrode Reference Panel ERP 27 It will describe intended usage practices as well as general product operation and troubleshooting It is not recommended that you use this product in a manner not described in this manual Improper use of this product can damage your equipment or cause the death of your test subject If your situation requires special usage of the ERP 27 contact Neuralynx to make sure your setup will not cause any unintended side effects Neuralynx 406 585 45
13. gure 5 1 Rack Mount Screw with Nylon Washer Neuralynx Inc ERP 27 User Manual Page 12 Rev 1 21 Last Revised April 20 2009 The number of ERP 27s will vary depending on your Cheetah system This diagram shows a Cheetah32 setup with one ERP 27 The ERP 27 should be mounted at the top of the rack system using four screws as shown When mounting L8 amplifiers beneath the ERP please ensure a 1 gap between the bottom of the ERP and the topmost L8 Table 5 ERP Rack Mounted Figure 5 2 ERP Rack Mounted 6 Cable Connection Cable connection is the most important part of the end user setup process If the ERP 27 is not cabled properly you may have poor referencing and possibly unusable signals leaving the ERP No error logs can be used to determine cabling issues so please read this section carefully NOTE Make sure that the L8PS is turned OFF while making cable connections Neuralynx Inc ERP 27 User Manual Page 13 Rev 1 21 Last Revised April 20 2009 Step 1 Lynx 8 Power Supply Cable Connection and Testing From the Lynx 8 Power supply connect the PSC five pin three wire connector to the ERP power connection labeled 75 15 Gnd on the back of the ERP board Turn ON the Power Supply then turn ON the ERP Verify that the power supply LEDs labeled 75 OK and 15 OK and the ERP LEDs labeled V V Z and are all green Turn OFF the ERP and then turn OFF the power supply Figure 6 2 Normal Power On for ERP 27
14. ie Neuralynx High Density Electrophysiology M Recording Systems Panel Reference Connections A e Neuralynx Inc Tucson AZ USA ERP 27 User Manual Patch Panel Referencing Document Revision 1 21 Last Revised April 20 2009 Neuralynx Inc 105 Commercial Bozeman MT 59715 Phone 406 585 4542 Fax 406 585 9034 www Neuralynx com support Neuralynx com Table of Contents Le DOCUMENT Overview sicaire a GE BR eR Be ES 3 2 HERP eZ V Overview nrin i ate abl suse aiatedeal de Ra smocshen ct Seach ie 4 3 Front Panelssisocisusiinssiin siase siina need Ade eee E ee dle cdah ee 5 4 Back Panel Oyervie Wnei ii a EE SKE TEA Ra Si 8 Al Headstage Modifications oirin a E EE E Aan 11 5 Mouhtihgs nenian e E E T E E R TA 12 G Cable Connection aasien n e aa a e a aiios 13 T SUS athe ERPE T T a E E EE E E E E E Leah i 17 About Bamana Connectors aoier nEn aa nT SE EO AR TTEA SAE DRET 17 9 Normal Referenc Seip yicides cs aces acduecstedes sensasntncegeta e aSa a iaae i oa E eaS 17 10 Using a Quiet Headstage Signal as Reference ss sssessesssessseressseessressersseesseeesseee 18 11 Using Multiple Amplifier Settings on a Single Headstage Signal ee 20 12 Inputting Non Headstage Signals into the ERP 27 o oo eee eeeeeeeeeeeeeeenceenaeeneeees 21 13 Amplifier and Analog to Digital Channel Mapping cc ceeeceeeeseeceeeeeceeeeeeeneeeees 22 t4 PSA A scsi do elev aies cal buenas sai ea tae hens race
15. ing Multiple Amplifier Settings on a Single Headstage Signal The ERP 27 is hard wired to only give one output for each headstage signal This only allows for one amplifier setting per headstage signal If you want to record the data for a single headstage signal using multiple amplifier settings i e to record a signal using narrow band filters for spikes and wide band filters for CSC you will need to make use of the CSC Signal Inputs Banana wires and switch positions are denoted the same way as in the last example Only relevant connections for the scope of this example are shown Headstage Electrode Signals Continuously Sampled Channel Amplifier Connections Ai 2345678 9001172 RP 2 34 5 6 7 CSC EEG Reference Patch Panel po ore for Neuralynx HS 27 Series D A a a E a E E E Headstage Preamp Boards and Lynx 8 Amplifier Systems B 2 3 4 5 6 7 8 9 0 11 12 z erence Connections eee C cc C ee Neuralynx Inc Tucson AZ USA saktan Sinab HS Power Control Figure 11 1 Using a signal multiple times In this example we want to record A72 as a spike channel a narrow band CSC to show all the data between spikes and a wide band CSC to show unfiltered data coming in on A12 All we have to do in order to accomplish this task is to make a banana wire with three connectors and connect the cable between A7 CSC1 and CSC2 Just to show that it is possible we are referencing CSC2
16. ing the wire in a very simple manner Because of this the banana plug plastic cover may become inadvertently loosened if you remove the plugs with a counter clockwise twisting motion Therefore we advise you to make a habit of applying a slight clockwise force when inserting and removing the plugs from the panel jacks This slight force should not be enough to cause the banana plug body to rotate within the panel jack Using strong twisting force on the banana wires will cause long term damage to the interior metal connection pieces 9 Normal Reference Setup Although the ERP 27 allows you to use any signal as a reference we have found that there is a setup that tends to provide the best noise reduction This setup simply involves connecting the Extra Headstage Inputs and PGnd to the Panel Reference Inputs with the provided small banana jumpers as shown below Neuralynx Inc ERP 27 User Manual Page 17 Rev 1 21 Last Revised April 20 2009 Panel Reference Connections A B R ee 2 A o R A d KEN k Tel a Ref N Figure 9 1 Typical Reference Setup The Headstage Signal Reference Selectors should generally be in the A position as this signal is usually the best representation of the normal background noise signals present The D or AGnd reference signal is best used in the following situations Using the HS 16 headstage which does not have a specific Ref channel All other available reference electrodes have increased in i
17. mpedance or have gone bad All references have single unit activity When using AGnd be aware that this signal is not buffered by the headstage If you need to use this signal as a reference it is a good idea to route the AGnd signal on the EIB through EZ or E2 on the EJB and then use the E signal as the reference These settings are suggested based on the majority of experiences your typical referencing may need to be different Try experimenting with reference selection in your system to find out what gives you the best results NOTE It is possible to position a selector switch between reference selection positions This is because the switches do not connect between positions which ensure that the reference sources will not be shorted during selector switch operation Therefore make sure that when a switch is moved that you do not leave the switch positioned between reference sources If this happens this will leave the reference to the respective headstage signal open not connected to any reference source and the result will be a very noisy recording channel at the output of the amplifier 10 Using a Quiet Headstage Signal as Reference When all of the typical reference signals do not reduce noise to a point where your input signals are usable you need to look elsewhere for reference selection The ERP 27 provides this flexibility If after looking at the activity of the implanted electrodes you notice a headstage signal that seems to hav
18. nction identically to the Panel Reference Inputs However the external reference input is only connected to the selector switch that is directly above the banana jack It is not routed through the entire panel as the panel references are You may wish to use these inputs if you are recording a differential stimulus or a signal that has its own reference that differs from any of the other references already connected to the ERP 27 4 Back Panel Overview Trt ss E Power ERS Connection 5V Fuse si 00o 0 0 5V Fuse Figure 4 1 Back Panel Power Connection LA lt Headstage Tether Connection Headstage Signal Outputs F CSC Signal Outputs The ERP 27 is designed to be powered from the Lynx 8 Power Supply L8PS sold separately The power input circuitry on the ERP 27 provides headstage voltage regulation and current monitoring The headstage power application is electromechanically delayed for 20ms after both the and 5V power supplies become fully charged The ERP 27 uses the same power connection as the L8 amplifiers The pin breakouts are as follows Neuralynx Inc ERP 27 User Manual Page 8 Rev 1 21 Last Revised April 20 2009 5 V Figure 4 2 Power Connection Pin Breakout 5V Fuse and 5V Fuse These fuses are used to protect the ERP 27 ci
19. nd These connections can be made with the short banana plug jumpers generally shipped with the ERP 27 Neuralynx Inc ERP 27 User Manual Page 5 Rev 1 21 Last Revised April 20 2009 Extra Headstage Signal Outputs As explained in the ERP 27 overview our 27 channel headstages actually have two extra EEG signals and one extra reference signal that are sent from the headstage to the ERP 27 The fourth signal AGnd will be explained below These extra signals are output through the banana jacks Unlike the Headstage Signal Outputs the ERP 27 is not hard wired to send these signals to the L8 amplifiers Although these signals can be used for other purposes the general usage is as follows Table 1 Signal Descriptions Signa l Description Reference electrode This is generally an inactive area of the brain that can be used to subtract the brain s background noise from the other electrodes Extra EEG channel 1 Can be used to either monitor a signal that will not be used for E1 recording or for a signal that is present in surrounding electrodes that you wish to subtract via referencing E2 Extra EEG channel 2 Same as E1 This is the HS 27 ground commonly referred to as Animal Ground This signal is carried on a dedicated non current carrying conductor directly from the HS 27 through the tether cable to the ERP 27 This is intended to be the best representation of the voltage on the HS 27 ground and the animal skull Ref AGnd
20. r to the 37 pin connector on the tether adapter The 50 pin connector should connect to the Headstage Tether Connection on the back of the ERP 27 as shown below Figure 6 6 Tether Adapter Connected The ERP 27 is now ready to use There are other steps required to use the complete Cheetah Acquisition System but they will not be covered in this manual Please see the appropriate documentation for your system for more details Neuralynx Inc ERP 27 User Manual Page 16 Rev 1 21 Last Revised April 20 2009 7 Using the ERP 27 After you have the Cheetah Acquisition Hardware and Software running you will need to be able to use the ERP 27 in order to actually record signals There are many things that the ERP 27 can do other than simply pass signals from the headstage to the L8 amplifiers In this chapter we will show you what a generic setup looks like as well as some of the common advanced setup techniques WARNING Using the ERP 27 in ways not described in this manual is not recommended please contact Neuralynx before using any non standard setup 8 About Banana Connectors The banana connector wires are assembled without soldering the wire to the metal banana plug The un insulated end of the wire is inserted through the center of the metal plug body and the plastic cap is screwed on which when tightened secures the end of the wire to the metal plug body This method reduces the overall cost and provides you the flexibility of chang
21. rcuitry from electrical surges If either of these fuses needs replacing please use 250mA 250V fast blow fuses When a fuse has blown the corresponding Fault indicator on the front of the panel will turn red Neuralynx Inc ERP 27 User Manual Page 9 Rev 1 21 Last Revised April 20 2009 Stimulus Connections This 10 pin connector contains the two differential stimulus channels that are connected through the tether and headstage to the electrode interface board EIB The stimulus channels are designed for delivery of an isolated current to a separate stimulus electrode These four signals are connected to the EIB without any other connections to avoid interference with stimulus signal delivery The ERP 27 is usually shipped with a stimulus plug connected to this socket The stimulus plug is a resistor pack that shunts the headstage power to the stimulus pins The resistors are placed at the ERP 27 to give current limiting protection in the event of tether damage i e an animal chewing through the tether With the stimulus plug in place the headstage power being sent down the tether can be used to power LEDs for use with a Cheetah VT video tracking system please refer to Headstage Modifications and Table 4 for updated information on this section of the ERP 27 WARNING When connecting the stimulus channels to a stimulus electrode in the brain the stimulus plug MUST be removed before turning on the ERP 27 power Failure to remove the stimulu
22. row of banana jacks and appending the number underneath the banana jack Example The leftmost banana jack on the top of the ERP 27 corresponds to the A signal from the headstage Headstage Signal Reference Selectors These five position slide switches are used to select the reference signal for the corresponding headstage channel the banana jack above the switch The switch positions A B C D E correspond to the Panel Reference Inputs The headstage signal will be sent with the reference signal to the input of the L8 amplifier for conditioning and amplification Example If you position the reference selector switch under the A banana jack to D the ERP will send the signal from the headstage on A along with the signal that is being input into D to the L8 amplifier The amplifier will amplify and output the difference between A and D to send a single signal to the computer for acquisition NOTE If there is no signal present on the selected reference the headstage input will be referenced to whatever input noise is present on the Panel Reference Input banana jack Panel Reference Inputs These banana jack inputs determine the signals used for reference selections A B C D and E throughout the ERP 27 You may connect any signal max 20V min 20V to these inputs However these inputs are most commonly connected to the Extra Headstage Signal Outputs directly below each Panel Reference Input A gt Ref BOE C gt E2 D gt AGnd E gt PG
23. rrently being used by your system is the only uncommented no line in the electrode setup section of the Cheetah cfg file Both the electrode setup file and Cheetah cfg file are located in your C Program Files Neuralynx Cheetah directory For more information on setup files see Setup File Command Reference under Cheetah s Help menu Standard setups for the systems in this guide are as follows These tables should be read as If you have ERP Connection connected to the front of Amplifier Number then ERP Channels map to Analog to Digital Channels Table 6 Cheetah 16 Amplifier Number ERP Channels ERP Connection Analog to Digital Channels 2 SEE NOTE SEE NOTE 3 15 1 SEE NOTE SEE NOTE 0 7 NOTE Cheetah16 systems do not have pre assigned ERP channels They can have any two of the Cheetah 32 ERP channels assigned to the two amplifiers by changing the JAMP connection to the back of the ERP Remember the ERP connection has nothing to do with the A D channel the A D channel is determined by the amplifier that the ERP is connected to Table 7 Cheetah 32 Amplifier Number ERP Channels ERP Connection Analog to Digital Channels 4 CSC1 CSC8 JAMP4 24 31 3 B5 B12 JAMP3 16 23 2 AQ B4 JAMP2 8 15 1 A1 A8 JAMP1 0 7 Neuralynx Inc ERP 27 User Manual Page 22 Rev 1 21 Last Revised April 20 2009 Table 8 Cheetah 64 ERP Connection Analog to Digital Amplifier Number ERP Channels ERP ERP Channels 8 CSC1
24. s aca ea ess daw ee ana See sas 23 List of Figures and Tables Figure 3 1 ERP 27 Front Panel Oyervie ws a 2 succsesi Sosnides teases ene sieeetel ewes 5 Figure 4 1 Back Panel niiet nna e a a peat A A aula ante 8 Figure 4 2 Power Connection Pin Breakout eesseseeeseseeesesesesesseserssresseseresressessresresseeee 9 Figure 4 3 Stimulus Connection Pin Breakout esseseseeseeesesresseesresrersersresreesreseeseresee 10 Figure 4 4 Headstage Tether Connection Pin Breakout seseseeeeeereeserereerresresreerresee 11 Figure 5 1 Rack Mount Screw with Nylon Washer sseeseeseseeseeseeeseerersessreereesresseseresre 12 Figure 5 2 ERP Rack Mounted esseeseseeeesessseseeeseesseserrsresseserestesseseresrensessresresseeseeseeesee 13 Figure 6 1 Power Supply Cable PSC connection ssseeseesesessesessersresrersersresrrssreseeseresee 14 Figure 6 2 Normal Power On for ERP 27 22 45 6420 Ai RS GL ce ees 14 Figure 6 3 J AMP cable connections e sessesseesesereseseesresseserssresseesresrenseseresreeseeseseeesee 15 Figure 6 4 L8 Amplifier connections sseseseesseseesseseesressesersstessesstsrtessesstesresseeseeseresee 15 Figure 6 5 Tether Adapter MiCcTO esesesseseseseeseseessrserrsresseserssresseserestensesstesresseeseeseresee 16 Figure 6 6 Tether Adapter Connected esseseesseeeeesesssesrtsstseresresstesresrersessresreeseeseeseeesee 16 Figure 9 1 Typical Reference Setup sesseessesseesesreesrssresress
25. s plug may cause your subject to sustain permanent brain injury or death The pin breakout of the stimulus connector is as follows amp amp 2 Q Tea Figure 4 3 Stimulus Connection Pin Breakout Table 3 Stimulus Breakout Table Pin Label Description 1 S1SRC Source stimulus signal 1 2 S1RET Return stimulus signal 1 3 S2SRC Source stimulus signal 2 4 S2RET Return stimulus signal 2 5 NC Not connected 6 NC Not connected 7 NC Not connected 8 NC Not connected 9 5V 5V Headstage Power 10 5V 5V Headstage Power Neuralynx Inc ERP 27 User Manual Page 10 Rev 1 21 Last Revised April 20 2009 4 1 Headstage Modifications Recently a new revision of the Neuralynx Headstages was released with a modified board layout of the Stimulation and Video Tracking traces In the past a stimulation plug was needed to power all Video Tracking LED s on the Headstages The HS 36 and the HS 18 Cooner were modified so that this plug is no longer needed If you have an older revision Headstage please continue to use the stim plugs as before Please refer to Table 4 for reference Table 4 HS Stim Plug Requirements Headstage HS STIM Plug Requirements for Video Tracking LED s HS 36 HS 27 HS 18 Cooner FWT HS 36 STIM Plug needed HS 27 STIM Plug Needed HS 36 STIM Plug needed on Revision 2 0 or earlier on all HS s on Revision 2 0 or earlier Headstage Tether Connection This connector is a 50 pin 3M
26. to C instead of A As far as the ERP is concerned anything plugged into one of the CSC inputs is a completely separate signal The only thing you need to remember about this setup is that CSC and CSC2 are going to be output through JAMP4 in the back of the ERP 27 whereas A 2 will be output through JAMP2 Once you have the Cheetah software setup to recognize these signals you can set the L8 amplifiers to filter all of these signals in different ways You can see the help file in the Cheetah software for more information on writing Cheetah configuration files Neuralynx Inc ERP 27 User Manual Page 20 Rev 1 21 Last Revised April 20 2009 12 Inputting Non Headstage Signals into the ERP 27 Sometimes it might be desirable to record an external signal along with all your neurodata for experiment monitoring purposes i e a differential stimulus signal This can be done using the CSC Signal Inputs Many external signal generators have their own reference so you will need to make use of the External Reference Inputs Headstage Electrode Signals A 99999999999 ER a ee ee ae ee SSE EE ee Neuralynx Inc Tucson AZ USA Continuously Sampled Channel Amplifier Connections Dom 09099 9 Qese a a eS N A ee P R B EEG Reference i s m a a a a a a m a a a m r Headstage Preamp i and Lynx 8 Amplifier F E al Ri Panel Reference Connections HS Power Control Headstage Signals Figure 12 1 Inputting
27. why even talk about the CSC inputs Good question Remember that we want to view the signal on Al in two different ways but there is only ONE A1 signal output from the ERP and it does so through JAMP1 In order to get Al out of the system as TWO different signals we need to make use of the ERP s CSC channels Since the ERP s CSC channels can accept any signal input through their banana connections we can utilize the banana connector under Al to connect A1 to one of the CSC connections Let us use CSC1 Ok now we have A1 connected to CSC1 This now gives us TWO signal outputs for Al through the normal Al output on JAMP1 and through the CSC1 output on JAMP4 remember these mappings are hard wired into the ERP and cannot be changed Great Except that we only have one amplifier and can Neuralynx Inc ERP 27 User Manual Page 23 Rev 1 21 Last Revised April 20 2009 only connect it to either JAMP1 or JAMP4 Don t worry we are not thwarted we still have seven more CSC inputs we can make use of Doing a bit of wire cutting and splicing we can turn the two headed banana cable into a three headed banana cable Now we can connect the three heads of the cable to Al CSC1 and CSC2 This gives us THREE outputs for Al However this time we have two signals on the same ERP output cable CSC1 and CSC2 both leave the ERP through JAMP4 If we connect JAMP4 to the front of our amplifier we can now use those two signals inside of Cheetah If you h
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