User`s Manual
Contents
1. User s Manual CentriVap DNA Systems Models 7970010 7970011 I Please read the user s manual before operating the equipment To receive important product updates register your product online at www labconco com productreg html or return the attached card and receive FREE LabbyWear Labconco s Mascot Labby the LABster Protecting your laboratory environment LABCONCO Labconco Corporation 8811 Prospect Avenue Kansas City MO 64132 2696 800 821 5525 816 333 8811 Fax 816 363 0130 E Mail Labconco labconco com Home Page www labconco com Copyright Information Copyright O 2005 Labconco Corporation All rights reserved Labconco Corporation 8811 Prospect Avenue Kansas City Missouri 64132 The information contained in this manual and the accompanying product are copyrighted and all rights are reserved by Labconco Corporation Labconco Corporation reserves the right to make periodic design changes without obligation to notify any person or entity of such change CentriVap is a registered trademark of Labconco Corporation Warranty Labconco provides a warranty on all parts and factory workmanship The warranty includes areas of defective material and workmanship provided such defect results from normal and proper use of the equipment The warranty for all Labconco products will expire one year from date of installation or two years from date of shipment from Labconco whiche2. Chapter 4 Using Your CentriVap discusses the basic operation of your CentriVap Information on how to arrange the samples inside your CentriVap and select an operating cycle 18 included Chapter 5 Maintaining Your CentriVap explains how to perform routine maintenance on your CentriVap Chapter 6 Accessories for your CentriVap describes how to install the optional glass lid Chapter 7 Troubleshooting contains a table of problems you may encounter while using your CentriVap including the probable causes of the problems and suggested corrective actions Appendix A CentriVap Components contains labeled diagrams of the components of the CentriVap Appendix B CentriVap Dimensions contains comprehensive diagrams showing the dimensions for the CentriVap Appendix C CentriVap Specifications contains product specifications Wiring diagrams for both the 115V and 230V CentriVap are also included 2 Product Service Domestic 1 800 522 7658 International 816 333 8811 Chapter 1 Introduction Typographical Conventions Recognizing the following typographical conventions will help you understand and use this manual e Book chapter and section titles are shown in italic type e g Chapter 3 Getting Started e Steps required to perform a task are presented in a numbered format Comments located in the margins provide suggestions reminders and references e Critical information is presented in boldface type
3. PROG 1 PROG 2 PROG 3 to quick start the CentriVap The rotor will start the vacuum break valve will close and the vacuum pump will start after the rotor reaches operating speed Set point parameters can be altered at any time during a run by first selecting the parameter using the set point SELECT button and then pressing the INCREASE or DECREASE switch If the run time set point is used at the end of the set time an alarm sounds All functions cease Press STOP to terminate operation if the CentriVap has not already stopped itself When the evaporation is complete allow the rotor to stop moving lift the lid and remove the samples Product Service Domestic 1 800 522 7658 International 816 333 8811 Chapter 4 Using Your CentriVap Operational Notes When the CentriVap is turned on the CentriVap returns to the same mode RUN or 5 that it was in when the power was turned off If the CentriVap was in the RUN mode when the power was turned off when the power is turned on the CentriVap attempts to return to the programmed set points and continue the run To reset the timers to the original set point when running other than program 1 program 2 or program 3 press the start button and hold it for five seconds The display will indicate that the timer 18 reset Interrupting a cycle after it has begun At any time during a run the cycle may be stopped by pressing the
4. PROG 3 resets the timers to the original set point time and the CentriVap starts a new run Product Service Domestic 1 800 522 7658 International 816 333 8811 15 Chapter 4 Using Your CentriVap 16 To reset the timers to the original set point time when running programs other than program 1 program 2 or program 3 press the start button and hold it for five seconds The display will indicate that the timer is reset Operating the CentriVap CAUTION To avoid personnel injury Io Tor CA Never lift the lid if the rotor is moving Never attempt to reach into the chamber if the rotor is moving Never attempt to defeat the lid latch mechanism Do not operate the CentriVap if the lid is scratched or nicked or shows signs of damage Never lean on the CentriVap when it is operating Turn the CentriVap power switch Select a program or set the set point parameters Preheat the chamber if desired Place samples in vials Normally the vials should be no more than half full Place vials in a rotor Load the rotor with samples into the chamber Close the lid 7 Press RUN If the display was showing set point parameters it will change 10 11 to show actual parameters Press RUN again The S STOP in the display changes to R RUN The program on the display alternately displays R or the number of the program that is running If you intend to run program 2 or 3 simply press
5. Product Service Domestic 1 800 522 7658 International 816 333 8811 Appendix C CentriVap Specifications Wiring Diagram 230V Model Y A38 109 661 N d ZH 09 06 18 4 Nvu9vIQ 9NIMIM Q9NO28Y 1 Ud 338005 3 04 MONJ 193NNO2SIQ ONINYV M 00085891 4 SS3NNYH 123433 11 831 WOSN3S 4831 00 140 15008 1 W101 SLIVA 009 831V3H 15008 1v3H m 2 008099 N d won Broa IT 174100 380815 01 05 N d 9 1021 Q1 035833 0003105 201 01 r 0 II Tete ERPE iind Product Service Domestic 1 800 522 7658 International 816 333 8811 32 Evaporation Rates Appendix C CentriVap Specifications Tube Size Numberof Sample Heater Time to Dry Rate Overall ml Samples Size ml Temp C min ml min Methylene Chloride 50 12 25 45 80 3 75 15 18 10 45 45 4 00 Toluene 50 12 25 45 204 1 47 50 12 25 100 96 3 13 15 18 10 45 119 1 51 15 18 10 100 42 4 29 Acetonitrile 50 12 25 45 184 1 63 50 12 25 100 81 3 70 15 18 10 45 106 1 70 15 18 10 100 52 3 46 1 5 132 1 45 55 2 40 1 5 90 45 47 1 92 1 5 132 1 60 37 3 57 1 5 132 1 75 33 5 74 1 5 132 1 100 21 6 29 Methanol 50 12 25 45 233 1 29 15 18 10 45 141 1 28 1 5 132 1 35 88 1 50 1 5 132 1 45 64 2 06 1 5 132 1 60 50 2 64 1 5 90 1 75 38
6. International 816 333 8811
7. equipment may be governed by various Federal State or local regulations All users of this equipment are urged to become familiar with any regulations that apply in the user s area concerning the dumping of waste materials in or upon water land or air and to comply with such regulations L8 Product Service Domestic 1 800 522 7658 International 816 333 8811 11 CHAPTER 4 UsiNc Your After your CentriVap has been installed as detailed in Chapter 3 Getting Started you are ready to begin using your CentriVap Read this chapter to learn how to Set operating parameters e Operate the controls Properly select and position glassware inside your CentriVap e Understand the display e Interrupt a cycle after it has begun CAUTION Do not use the CentriVap in a manner not specified by the manufacturer refer to Appendix C CentriVap Specifications The electrical protection properties of the CentriVap may be impaired if the CentriVap is used inappropriately CAUTION Do not store or stack supplies or equipment on top of the CentriVap ES m 12 Product Service Domestic 1 800 522 7658 International 816 333 8811 Chapter 4 Using Your CentriVap Planning Thoroughly understand procedures and the equipment operation prior to beginning work The unique performance of the CentriVap is dependent upon the proper balance of heat vacuum and centrifugal force If the proper balance is not established it i
8. Heater power 300 watts Environmental Conditions Indoor use only Maximum altitude 6562 feet 2000 meters Ambient temperature range 41 to 104 F 5 to 40 C Maximum relative humidity 8096 for temperatures up to 88 F 31 C decreasing linearly to 50 relative humidity at 104 F 40 C Main supply voltage fluctuations not to exceed 10 of the nominal voltage Transient overvoltages according to Installation Categories II Overvoltage Categories per IEC 1010 Temporary voltage spikes on the AC input line that may be as high as 1500V for 115V models and 2500V for 230V models are allowed Used in an environment of Pollution degrees 2 1 e where normally only non conductive atmospheres are present Occasionally however a temporary conductivity caused by condensation must be expected in accordance with IEC 664 Product Service Domestic 1 800 522 7658 International 816 333 8811 Appendix C CentriVap Specifications Wiring Diagram 115V Model Y 006 66 N d ZH 09 05 ASI 18 4 2 9NIMIM O9NO928V 1 N SNINSVM 56555650 00096 N d WOSN3S 4131 g moor W31V3H NIVA 7140 15008 1Y3H 15008 1V3H 00808991 N d 9 380815 nois 1061 20 089 N d 100 V NO QION310S 201 011 Q 838440 10013 lb 1311 0 dMnd MnnovA 31
9. loading samples 5 Increase Button When pressed the last selected set point will increase 6 Decrease Button When pressed the last selected set point will decrease 7 Set Point Select Button To select a parameter to change press the select button Arrows on the display will point to the parameter that may be altered 14 Product Service Domestic 1 800 522 7658 International 816 333 8811 Chapter 4 Using Your CentriVap Operating the CentriVap Controls Preheat To preheat the chamber press the PREHEAT button The display will show SP XXX ACT XXX Press the increase or decrease button until the desired set point SP 18 displayed The actual chamber temperature ACT 18 displayed to the right When power is being supplied to the heaters a bar under the actual temperature will illuminate The chamber will continue to preheat until either the button 18 pressed again or the RUN button is pressed Select existing program Operating parameters can be stored in memory so protocols can be repeated Nine programs can be stored To select a program press the set point SELECT button until arrows point to the program number indicating that this set point can be run or altered To change the program number press the increase or decrease button until the desired program number is displayed When the program number is changed all its set points change also to indicate the last entered p
10. 100 Relay Vacuum Pump 230V 10 1 7826000 Switch Latch 11 1 7478700 Solenoid Latch 12 1 7403701 Display 13 1 7398400 Label Switch Pad 14 1 7452600 Gasket Lid 15 1 7452500 144 16 42 2 inch 7451700 Extrusion 17 2 7452200 Hinge Bracket Assembly 18 1 1289300 Circuit Breaker 115V 18A 2 1289308 Circuit Breaker 230V 19 1 7450400 Vacuum Pump 115V 19A 1 7450401 230 20 2 7450200 20 2 7450300 Gasket Jar Trap Not Shown 1 1334500 Power Cord 115V Not Shown 1 1338000 Power Cord 230V 21 1 1302300 Switch 22 1 7397601 Coupling Insert 23 1 7441500 Fan Product Service Domestic 1 800 522 7658 International 816 333 8811 Appendix A CentriVap Components Product Service Domestic 1 800 522 7658 International 816 333 8811 28 APPENDIX B CENTRIVAP DIMENSIONS LID SHOWN IN BOTH OPEN AND CLOSED POSITIONS INCH CENTIMETERS Product Service Domestic 1 800 522 7658 International 816 333 8811 29 30 APPENDIX C CENTRIVAP SPECIFICATIONS This Appendix contains technical information about the CentriVap including specifications environmental operating conditions wiring diagrams and evaporation rates Electrical Specifications Nominal amperage for 115V CentriVap model 7970010 including vacuum pump 5 5A Nominal amperage for 230V CentriVap model 7970011 including vacuum pump 2 8 Frequency all models 50 60 Hz Phase Single
11. 2 37 1 5 132 1 75 39 3 38 1 5 132 1 100 23 5 28 Water 50 12 25 45 1397 0 21 50 12 25 100 445 0 67 15 18 10 45 841 0 19 1 5 132 1 45 427 0 30 1 5 132 1 60 303 0 44 1 5 132 1 75 196 0 67 1 5 132 1 100 117 1 13 Product Service Domestic 1 800 522 7658 International 816 333 8811 33 DECLARATION OF CONFORMITY Application Council Directive s 73 23 EEC 89 336 EEC Standard s to which conformity is declared EN61010 EN55014 EN55104 Manufacturer s Name Labconco Corporation Manufacturer s Address 8811 Prospect Avenue Kansas City MO 64132 USA Importer s Name See Shipping Customs Documents Importer s Address See Shipping Customs Documents for your equipment Type of Equipment Laboratory Equipment Model No CentriVap DNA System 7970011 CentriVap Benchtop Concentrator 7810011 7810013 7810015 CentriVap Cold Trap 7811030 7460030 CentriVap Mobile Console 7812011 7812014 Serial No Various See Individual Declaration Year of Manufacture 2005 and Subsequent I the undersigned hereby declare that the equipment specified above conforms to the above Directive s and Standard s See individual Declaration of Conformity which will be signed by the importer for your country Place Signature Date Full Name Position An individual version of this declaration is included with your shipping customs documentation Labconco P N 36960 04 Rev D ECO C877 Product Service Domestic 1 800 522 7658
12. CCESSORIES FOR YOUR CENTRIVAP Installing a Glass Lid Installing a Secondary Chemical Trap Installing a Vacuum Gauge Installing a CentriZap Strobe Light CHAPTER 7 TROUBLESHOOTING APPENDIX A CENTRIVAP COMPONENTS APPENDIX B CENTRIVAP DIMENSIONS APPENDIX C CENTRIVAP SPECIFICATIONS Electrical Specifications Wiring Diagram Concentrator 115V Wiring Diagram Concentrator 230V Evaporation Rates DECLARATION OF CONFORMITY 14 15 16 17 17 18 20 22 22 23 24 25 21 29 30 30 31 32 33 34 CHAPTER 1 INTRODUCTION Congratulations on your purchase of a Labconco CentriVap DNA Centrifugal Concentrator Models are available for operation on 115V or 230V The CentriVap Concentrator uses centrifugal force with heat and vacuum to rapidly dry precipitates of DNA and RNA samples Centrifugation eliminates bumping and foaming as vacuum is applied and also concentrates the solute in the bottom of the vial This allows recovery of solutes from sample volumes as small as a few microliters The concentrator is equipped with a 300 watt heater to speed evaporation by warming the chamber during processing A timed outlet for the vacuum pump connection delays the start of the pump until the rotor reaches operating speed A safety switch prevents the concentrator from starting when the lid is in the open position and a latch prevents the lid from being opened while the rotor is turning The compact low profile design allows the concentra
13. STOP button This shuts off all operating functions After the rotor stops the lid may be opened If it is necessary to re start the CentriVap close the lid and press RUN The CentriVap resumes operation at the same set point parameters and the timer continues to count down from the time at which the CentriVap was stopped Product Service Domestic 1 800 522 7658 International 816 333 8811 17 18 CHAPTER 5 MAINTAINING YOUR Under normal operation the CentriVap requires little maintenance The following maintenance schedule is recommended As needed l Daily Clean up all spills remove liquids from the chamber Clean or decontaminate all surfaces using agents suitable for the substance spilled Clean lid and gasket using soft cloth sponge or chamois and a mild non abrasive soap or detergent At the end of the run liquid collected in the glass traps should be removed To empty a trap unscrew the glass bowl from the head Dispose of the liquid in accordance with all codes Reinstall the glass bowl by screwing it back on to the head If the media in the optional clear canister has changed color discard and replace the insert with a new insert For the radiochemical trap insert no indicator exists therefore it should be discarded after each use In radioactive applications the system should be monitored with a Geiger counter To prolong the life of the DNA vacuum pump and to op
14. arameters for that program Store frequently run protocols in program 1 2 3 Then by pressing PROG 1 PROG 2 or 3 the stored program will be initiated without having to press any other button Pressing just the one button starts the rotor the heater the timers and the vacuum pump Change Temperature Set Point To change the Temperature set point press the set point SELECT button until arrows point to the Temperature set point which can be changed from OFF up to 99 by pressing the increase or decrease buttons The set point is 100 C if the display shows The last entered set point is stored in memory Change Run Time or Heater Time Set Point To change the time set point press the set point SELECT button until arrows point to the Heater Time or Time set point which can be changed from 1 to 999 minutes by pressing the increase or decrease buttons If it is desired to have the CentriVap run continuously without alarming at the end of a time period press the increase button until the Time display says The last entered set point is stored in memory If during a run the STOP button is pressed the timers remember the time at which stop occurred If RUN is then pressed the timers continue to count down from the time at which they were stopped If you are running program 1 program 2 or program 3 pressing PROG 1 PROG 2 or
15. ccommodate your needs If the solvents used in the CentriVap degrade the acrylic lid it may be replaced with an optional glass lid to gain added chemical resistance You may wish to add a secondary trap to trap vapors exhausted from the vacuum pump To observe the samples while they are processing an optional CentriZap Strobe light may be installed Read this chapter to learn how to Install a glass lid e Install a chemical trap e Install an accessory vacuum gauge e Install a CentriZap strobe Product Service Domestic 1 800 522 7658 International 816 333 8811 Chapter 6 Accessories for Your CentriVap The following accessories are available for the CentriVap DNA Concentrator PART DESCRIPTION 7462900 Rotor DNA Holds 72 0 5 ml microcentrifuge tubes and 60 1 5 ml microcentrifuge tubes or 60 2 0 ml microcentrifuge tubes 7450700 Rotor 1 5 ml Holds 132 1 5 ml microcentrifuge tubes or 132 2 0 ml microcentrifuge tubes 7455000 Rotor 15 ml Holds 44 12 x 55 mm tubes or 36 12 x 75 mm tubes or 36 12 x 95 mm tubes or 36 13 x 75 mm tubes or 36 13 x 100 mm tubes and 24 16 x 100 mm tubes or 18 16 x 120 mm conical tubes or 18 17 x 95 mm tubes or 18 17 x 100 mm centrifuge tubes or 18 17 x 120 mm tubes 7455100 Rotor 12 13 mm Holds 40 1 5 ml microcentrifuge tubes or 40 2 0 ml microcentrifuge tubes and 16 12 x 55 mm tubes or 100 12 x 75 mm tubes or 64 12 x 95 mm tube
16. ces It has been designed for use with compounds as described in the United States National Electrical Code Class I Group D The heater may be programmed to run as hot as 100 C however the heater element may normally run at 110 C A thermal fuse limits the heater to a maximum temperature of 141 C It is important that the solvents used are compatible with these temperatures Do not evaporate solvents that have an autoignition temperature below 180 C Do not evaporate solvents that are classified as Group A B or C by the National Electrical Code Evaporate only non flammable or Group D solvents with autoignition temperatures 180 C or above Use of other compounds could cause an explosion 10 Product Service Domestic 1 800 522 7658 International 816 333 8811 Chapter 3 Getting Started CAUTION Solvents used in the CentriVap may be flammable or hazardous Use extreme caution and keep sources of ignition away from the solvents When using flammable or hazardous solvents both the CentriVap and the vacuum pump should be operated inside a fume hood d If a sample is spilled in the chamber it must immediately be cleaned up Hazardous materials such as strong acids or bases radioactive substances and volatile organics must be handled carefully and promptly cleaned up if spilled Do not store flammable or hazardous solvents within 12 inches 300 mm of the CentriVap IMPORTANT The disposal of substances used in connection with this
17. derate Degradation Questionable use D Severe Degradation Infrequent use recommended immediate thorough cleaning required e Diaphragm vacuum pumps sold by Labconco have wetted parts either made from Teflon or protected by Teflon coatings and are suitable for nearly all procedures Product Service Domestic 1 800 522 7658 International 816 333 8811 9 Water Chapter 3 Getting Started When using compounds in the CentriVap that are hostile to the materials of construction it is imperative that the equipment is appropriately maintained e After each run clean up all residues spills and materials that might have splashed in the chamber using agents suitable for the substance involved Ifthe compounds used attack acrylic consider using the optional glass chamber lid Contact Labconco for ordering information e foptional secondary traps are used monitor their condition often and replace them when they are saturated A new acid trap is off white and changes color to purple when used up A new moisture trap is blue and changes color to pink when it is used up The solvent trap molecular sieve does not change color when saturated so extra care must be taken to determine when a replacement cartridge should be installed Solvent Safety Precautions CAUTION The CentriVap is not classified as explosion proof It has been designed with safety as a primary consideration and should be used in a prudent manner using good laboratory practi
18. ed to be chemical resistant to most compounds that are commonly used in the concentration processes that are performed in it However by necessity the CentriVap is comprised of a number of different materials some of which may be attacked and degraded by certain chemicals The degree of degradation is obviously dependent on the concentration and duration of exposure Some major components of the CentriVap that are susceptible to degradation are as follows Acids Bases Solvents e r lt 9 m S 2 QNIN NE x 5 o 8181 5 S 5 Elg S 5 Q 2 2 4 313 51521 Jel _ 6 8 2 9 5 5 lt 5 e g u 9 19 B e d 2125 5153 s 8 161214 2 1 52281252 9 92 56 5 3 2 5151815 15151 15 219 gt gt o o zictio gog z zic o lo g 8 g SI 2 x S lt 1 gt gt 5 E 919 16 a s s 2 s s s s COMPONENT MATERIAL 5 28 1 1 12 0 lt lt lt oj 12 RotorHub Acetal Der Ip fel Lid Chamber Acrylic c 0101 Dj jp Chamber Epoxy coated C C Aluminum ww Pe fet fel wees PETE EEE LE EE ET de LL LI 5 fe sma 12131125 1 51 11 213 2 434 232 122 Aluminum Rotor Shaft__ Stainless Steel D Bearings High Carbon Steel C Mo
19. he CentriVap using a length of suitable rubber hose The coupling insert fitting can then be pushed into the mating connector on the left side of the CentriVap located towards the rear The coupling insert fitting can be removed from the mating connector by pressing on the tab on the connector and then pulling out the insert fitting Product Service Domestic 1 800 522 7658 International 816 333 8811 Chapter 6 Accessories for Your CentriVap Installing a CentriZap Strobe Light An accessory strobe light is available to enable you to see the samples as they are rotating in the rotor Attach the holder to the right hand side of the CentriVap Concentrator using the screws provided Plug the connector on the strobe light harness into the receptacle on the back of the CentriVap marked STROBE OUTLET Peel the protective backing off the enclosed Velcro and attach it to the back of the vacuum pump housing Secure the power supply box on the harness to the Velcro Place the light in the holder To use the strobe light while the CentriVap is operating remove the light from its holder press the trigger and shine the light on the samples in the rotor Holder Screws Plug wire into Strobe receptacle Box secured with Velcro 24 Product Service Domestic 1 800 522 7658 International 816 333 8811 CHAPTER 7 TROUBLESHOOTING Refer to the following if your CentriVap fails to operate properly If the suggested c
20. iVap until you have checked all of the components and installed and tested the CentriVap CentriVap Components As previously mentioned the CentriVap DNA System is available in 115V or 230V Locate the model of CentriVap you received in the following table Verify that the components listed are present and undamaged Concentrator Catalog Product Description 7970010 CentriVap Concentrator 115V 7970011 CentriVap DNA Concentrator 230V Plus the Following Part Component Description 73977604 User s Manual 1334500 Power Cord 115V or 1338000 Power Cord 230V 7462900 Rotor If you do not recetve one or more of the components listed for your CentriVap or if any of the components are damaged contact Labconco Corporation immediately for further instructions Product Service Domestic 1 800 522 7658 International 816 333 8811 T Chapter 3 Getting Started Setting Up Your CentriVap After you verify receipt of the proper components move your CentriVap to the level and sturdy location where you want to install it There is no need to secure the CentriVap DNA Concentrator to the work surface Then follow the steps listed below Emergency Access Into the Chamber The CentriVap is designed to prevent access to the chamber in the event of a power disruption If it is necessary to open the lid when there is no electrical power connected to the CentriVap insert a small screwdriver or similar instrument into the small ro
21. in paragraphs that are preceded by the exclamation icon Failure to comply with the information following an exclamation icon may result in injury to the user or permanent damage to your CentriVap Important information is presented in capitalized type in paragraphs that are preceded by the pointer icon It is imperative that the information contained in these paragraphs be thoroughly read and understood by the user Product Service Domestic 1 800 522 7658 International 816 333 8811 CHAPTER 2 PREREQUISITES Before you install your CentriVap you need to prepare your site for installation Carefully examine the location where you intend to install your CentriVap You must be certain that the area is level and of solid construction In addition an exhaust means must be provided An electrical source must be located near the installation site Carefully read this chapter to learn The electrical supply requirements e The exhaust requirements Refer to Appendix C CentriVap Specifications for complete CentriVap electrical and environmental conditions specifications and requirements CAUTION The CentriVap has not been evaluated by an approval agency for the use of biological radio toxins or flammable liquids or materials Product Service Domestic 1 800 522 7658 International 816 333 8811 Chapter 2 Prerequisites Electrical Requirements The CentriVap Concentrator requires a dedicated grounded elect
22. ion in hose Lack of adequate vacuum Pump not on Liquid in glass trap Control valve open Leaks in lines or connectors or gasket Foreign material on lid gasket Latch optical sensor improperly calibrated Connect unit to proper electrical receptacle Correct electrical problem and reset circuit breaker by pressing button Close lid Reposition sample tubes Redirect hose to fume hood Contact Labconco Check pump Remove obstruction or replace hose See below Turn on pump Empty trap Check control valve Locate and repair Clean gasket and lid Unplug power cord Wait 10 seconds Plug in power cord Product Service Domestic 1 800 522 7658 International 816 333 8811 APPENDIX COMPONENTS The following pages list components that are available for your CentriVap The parts shown are the most common replacement parts If other parts are required contact Product Service Item Quantity Part No Description 1 1 7459000 Motor w Capacitor 2 1 7481700 Valve Vacuum Break 115 2A 1 7481701 Valve Vacuum Break 230V 3 1 7453500 Heater 115 1 7453501 Heater 230 4 1 1647106 O Ring Outer 5 1 1647105 O Ring Inner 6 1 7975902 Fuse High Temp Limiter 7 1 7456000 Harness Temp amp Speed Sensor 8 1 7479400 9 1 1289200 Relay Vacuum Pump 115 9A 1 1289
23. ith such regulations Labconco Corporation is held harmless with respect to user s compliance with such regulations Contacting Labconco Corporation If you have questions that are not addressed in this manual or if you need technical D assistance contact Labconco s Customer Service Department or Labconco s Product Service Department at 1 800 821 5525 or 1 816 333 8811 between the hours of 7 00 a m and 6 00 p m Central Standard Time Visit Labconco s web site at http www labconco com or email Labconco at labconco labconco com Part 7397604 Rev ECO C782 TABLE Or CONTENTS CHAPTER 1 INTRODUCTION About This Manual Typographical Conventions CHAPTER 2 PREREQUISITES Electrical Requirements Location and Exhaust Requirements Space Requirements CHAPTER 3 GETTING STARTED Unpacking Your CentriVap CentriVap Components Setting Up Your CentriVap Emergency Access Into the Chamber Exhaust Port Electrical Connection Rotor Installation Chemical Resistance Chart Solvent Safety Precautions CHAPTER 4 USING YOUR CENTRIVAP Planning Glassware Selection Loading Glassware Into the CentriVap Temperature Setting Guidelines Time Setting Guidelines WN Ann xO 34 12 13 15 13 13 CentriVap Controls Operating the CentriVap Controls Operating the CentriVap Operational Notes Interrupting a Cycle After it Has Begun CHAPTER 5 MAINTAINING YOUR CENTRIVAP CHAPTER 6 A
24. orrective actions do not solve your problem contact Labconco for additional assistance The following failure codes may appear on the display when problems are sensed by the internal self check routine CAUTION Disconnect power before corrective action is taken DISPLAY ERROR CODE Heat Sensor Close Lid Latch Fail Mem Fail P1 MOTOR ERROR CAUSE Sensor failure Connection failure Lid open Solenoid failure Switch or sensor failure Memory failure New memory IC chip Bad memory IC chip Defective motor Hall effect sensor failure Wire failure CORRECTIVE ACTION Replace sensor assembly Repair connection Close lid Check component Check connections Push program button 1 Push program button 1 Call Labconco Replace IC chip or control PCB Replace motor Replace sensor Replace wire Product Service Domestic 1 800 522 7658 International 816 333 8811 25 Chapter 7 Troubleshooting Other corrective actions for potential problems are as follows PROBLEM CAUSE CORRECTIVE ACTION Unit will not operate Excessive vibration Sample odor in lab Evaporation rate 18 reduced No vacuum poor vacuum Unit starts and shuts off Unit not connected to electrical power Circuit breaker blown Lid open Sample tubes not located symmetrically in rotor Vent hose exhausting into lab area Heater inoperable Vacuum pump failure Obstruct
25. r CentriVap is properly prepared you are ready to unpack inspect install and test your CentriVap Read this chapter to learn how to e Unpack and move your CentriVap e Set up your CentriVap e Connect the electrical supply source to your CentriVap Properly exhaust your CentriVap e Safely use solvents with your CentriVap carton allows for lifting with a mechanical lift truck or hand truck If you must lift the CentriVap manually use at least two 2 persons and follow safe 1 CAUTION The CentriVap Concentrator weighs over 69 Ibs 31 Kg The lifting guidelines Product Service Domestic 1 800 522 7658 International 816 333 8811 Chapter 3 Getting Started Unpacking Your CentriVap Carefully unpack your CentriVap and inspect it for damage that may have occurred in transit If your CentriVap is damaged notify the delivery carrier immediately and retain the entire shipment intact for inspection by the carrier The United States IMPORTANT Do not return goods without the prior authorization of Interstate Commerce Labconco Unauthorized returns will not be accepted Commission rules require that claims IMPORTANT If your CentriVap was damaged in transit you must file a 2 IP Ue elivery carrier claim directly with the freight carrier Labconco Corporation and its dealers fifteen 15 are not responsible for shipping damage days of delivery Do not discard the carton or packing material for your Centr
26. rical outlet This outlet requires a 15 Amp circuit breaker or fuse for models rated at 115V 60 Hz An 8 Amp circuit breaker or fuse is required for models rated at 230V 50 60 Hz 115V models are equipped with a 15 Amp NEMA 5 15P plug The power cord on 230V models is equipped with a NEMA 6 15P plug If this does not match with the available receptacle remove this plug and replace it with an approved plug of the suitable style It is recommended that an emergency switch for disconnecting the mains in the case of a malfunction be located remote from the CentriVap preferably outside the room in which the CentriVap is housed or adjacent to the exit from that room Location and Exhaust Requirements CAUTION The CentriVap DNA System should be located within a fume n hood if hazardous or flammable solvents are used In all cases regardless of the solvent used it is strongly recommended that the vacuum pump is vented in a fume hood An accessory secondary trap is available to minimize the exhausting of solvents into the atmosphere This does not however negate the need to exhaust the vacuum pump into a fume hood Failure to properly vent the CentriVap will expose personnel to potentially harmful chemicals Space Requirements Refer to Appendix C CentriVap Specifications for dimensional drawings of the CentriVap Product Service Domestic 1 800 522 7658 International 816 333 8811 CHAPTER 3 GETTING STARTED Now that the site for you
27. s or 100 13 x 75 mm tubes or 64 13 x 100 mm tubes 7455200 Rotor 50 ml Holds 32 1 5 ml microcentrifuge tubes or 32 2 0 ml microcentrifuge tubes and 12 28 x 115 mm conical tubes or 12 28 x 135 mm conical tubes or 12 28 x 140 mm conical tubes 7461900 Rotor 96 well plate Holds 4 Standard 96 well plates or 2 Deep well 96 well plates 7460900 Clear Canister Accommodates inserts listed below 7814800 Acid Trap Insert 7814900 Moisture Trap Insert 7815000 Radiochemical Trap Insert 7815200 Solvent Trap Insert 7456600 Glass Lid for Concentrator Direct replacement for standard equipment acrylic lid For use with chemicals that could craze acrylic 7464300 CentriZap Strobe Light For observing samples while the rotor is spinning Product Service Domestic 1 800 522 7658 International 816 333 8811 Chapter 6 Accessories for Your CentriVap Installing a Glass Lid Turn off the CentriVap Unplug the power cord from the wall receptacle Pull outward on the hinge pin knob Lift and remove the old lid To install the new lid reverse the process Installing a Secondary Chemical Trap An accessory secondary chemical trap is available to minimize the exhausting of solvents into the atmosphere Attach the bracket to the left hand side of the housing using the screws provided Attach the hose from the outlet on the vacuum pump to
28. s possible to damage or lose a portion of the sample Therefore if you are unfamiliar with the CentriVap or are attempting a new protocol it may be helpful to make a trial run that is void of the sample you are attempting to concentrate Glassware Selection Normally sample tubes should be filled no more than approximately half full Select the size of the sample tube so it is compatible with the rotor and the desired sample size Tubes should not be loose in the rotor Rotors are available with holes for various size tubes Refer to Chapter 6 Accessories for your CentriVap DNA Concentrator for available rotor sizes Loading Glassware into the CentriVap Smooth operation of the CentriVap is dependent upon proper balance of the machine Therefore if less than a full load of samples is run it is important to load samples into the CentriVap in a fairly symmetrical manner distributing the weight of the samples evenly in the sample rotor Temperature Setting Guidelines The evaporation rate achieved by the CentriVap is dependent upon a variety of factors These include the nature of the solvent the temperature and the pressure in the vacuum system As a general guideline to speed the evaporation process the CentriVap temperature should be set as high as possible as long as the temperature will not damage the sample or cause the sample to bump Time Setting Guidelines The CentriVap Concentrator has two timers The RUN TIME turns
29. the out connector of the canister housing Unscrew the clear bowl of the canister housing from the head Remove both the upper and lower caps from the filter cartridge and insert the small end of the cartridge into the hole in the center of the head Reinstall the clear bowl Clear Canister Bracket Hose 22 Product Service Domestic 1 800 522 7658 International 816 333 8811 Chapter 6 Accessories for Your CentriVap Be sure to use the proper cartridge for your application The moisture cartridge is used to trap water vapor The acid cartridge 18 used to trap acid vapors The solvent cartridge is used to trap solvent vapors and the radiochemical cartridge is used to trap radioactive waste When the media in the insert has changed color discard the insert and replace it with a new insert For the radiochemical trap insert no color indicator exists therefore it should be discarded after each use Use a Geiger counter to monitor the pump exhaust Warning This radiochemical cartridge does not meet NRC filter design recommendations After operating properly dispose of all hazardous materials in compliance with all applicable codes Labconco is not responsible for improper disposal of any materials Installing a Vacuum Gauge A user supplied vacuum gauge may be attached to the CentriVap to monitor the vacuum level Attach the vacuum gauge to the barb end of the coupling insert fitting that was supplied with t
30. the entire concentrator off after the user set period of time This stops the rotation turns off the heater and vacuum pump and bleeds vacuum from the system The heater may be turned off prior to the concentrator turning off so heat sensitive samples may be protected from exposure to excessive heat after the solvent has evaporated To turn the heater off prior to stopping the entire system enter a set time into the memory at the HEATER TIME When the programmed time expires the heater will turn off but the rotation and vacuum will continue until the run time expires Product Service Domestic 1 800 522 7658 International 816 333 8811 13 Chapter 4 Using Your CentriVap The tables in Appendix C CentriVap Specifications indicate approximate times required to evaporate various common solvents Actual times must be determined by the user The CentriVap can be set to alarm after a preset period of operation When the time expires the CentriVap will give an audible alarm and turn itself off CentriVap Controls The control panel for the CentriVap is shown below with a description about its function 1 Display The liquid crystal display LCD shows set point parameters and actual measured conditions 2 Program Buttons Used to initiate the start of a run with the use of just one button 3 Run Stop Button Used to start or stop a run 4 Preheat Button Used to turn on the heater to preheat the chamber prior to
31. timize its performance it is important to purge all liquid from within it When the DNA concentration process is completed open the CentriVap lid and push the rotor switch ON In ten seconds the pump will operate and will draw air through the system The rotor will not turn with the lid open The length of the liquid purge time is dependent on the volatility of the solvents used Use of water may require an overnight purge time Product Service Domestic 1 800 522 7658 International 816 333 8811 Chapter 5 Maintaining Your CentriVap Monthly 1 The rubber components on the CentriVap may eventually deteriorate and require replacement The effective life of rubber parts depends upon both their usage and the surrounding environment Check all rubber hoses and gaskets and replace any that show signs of hardening permanent set or deterioration 2 Using a soft cloth sponge or chamois and a mild non abrasive soap or detergent clean the glass lid 3 Using a soft cloth sponge or chamois and a mild non abrasive soap or detergent clean the exterior surfaces of the unit Liquid spray cleaners and polishes may be used on the exterior surfaces Do not use solvents to remove stains from the exterior surfaces as they may damage the finish Product Service Domestic 1 800 522 7658 International 816 333 8811 19 20 CHAPTER 6 Accessories For Your The configuration of your CentriVap be changed to a
32. tor to be placed on a bench top or other laboratory work surface All wetted parts within the pump are either Teflon Teflon coated or stainless steel A glass trap is provided to remove liquid from the system before it enters the pump and a second glass trap is provided on the outlet side of the pump to trap liquid expelled from the pump The microprocessor which controls the operation of the heater and the motor provides excellent regulation and reproducibility of protocols A manually preset timer can signal the end of the run Nine different protocols can be stored in memory so exact test parameters can be easily repeated Teflon is a registered trademark of DuPont Product Service Domestic 1 800 522 7658 International 816 333 8811 Chapter 1 Introduction About This Manual This manual is designed to help you learn how to install use and maintain your CentriVap Instructions for performing routine maintenance and making minor modifications to your CentriVap are also included Chapter 1 Introduction provides a brief overview of the CentriVap explains the organization of the manual and defines the typographical conventions used in the manual Chapter 2 Prerequisites explains what you need to do to prepare your site before you install your CentriVap Electrical and exhaust requirements are discussed Chapter 3 Getting Started contains the information you need to properly unpack inspect and install your CentriVap
33. und hole on the left side of the case near the top behind the control panel This will unlock the lid latch mechanism While holding the screwdriver in place raise the lid with the other hand CAUTION Never attempt to defeat the latch or open the lid while the CentriVap is running Personnel injury can result from moving parts and chemicals CAUTION It is recommended that the CentriVap DNA Concentrator be located inside a fume hood or other laboratory ventilation device if hazardous solvents are used in the CentriVap Exhaust Port It the CentriVap is not located in a fume hood attach one end of a 50 inch ID hose to the exhaust port on the left side on the vacuum pump glass trap exhaust port Clamp securely Route the other end to a fume hood or other laboratory ventilation device Electrical Connection Plug the power cord into the receptacle on the back of the CentriVap Concentrator and plug the other end into a suitable power receptacle Rotor Installation Place the rotor onto the shaft of the Concentrator Rotate the rotor slightly to engage the drive pin in the shaft with the slots in the rotor hub The top of the shaft should be in line with the top of the rotor hub INPORTANT Do not use a rotor if it shows any signs of damage Product Service Domestic 1 800 522 7658 International 816 333 8811 Chapter 3 Getting Started Chemical Resistance of CentriVap Components Your CentriVap DNA Centrifugal Concentrator is design
34. ver is sooner except the following e Purifier amp Delta Series Biological Safety Cabinets which carry a three year warranty from date of installation or four years from date of shipment from Labconco whichever is sooner e Carts carry a lifetime warranty e Glassware is not warranted from breakage when dropped or mishandled This limited warranty covers parts and labor but not transportation and insurance charges In the event of a warranty claim contact Labconco Corporation or the dealer who sold you the product If the cause 1s determined to be a manufacturing fault the dealer or Labconco Corporation will repair or replace all defective parts to restore the unit to operation Under no circumstances shall Labconco Corporation be liable for indirect consequential or special damages of any kind This statement may be altered by a specific published amendment No individual has authorization to alter the provisions of this warranty policy or its amendments Lamps and filters are not covered by this warranty Damage due to corrosion or accidental breakage is also not covered Limitation of Liability The disposal and or emission of substances used in connection with this equipment may be governed by various federal state or local regulations All users of this equipment are required to become familiar with any regulations that apply in the user s area concerning the dumping of waste materials in or upon water land or air and to comply w
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