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Catalogue Reproduction Technology Canine
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2. Animal Reproduction Technology Canine 1 minitube www minitube com Innovative ideas modern production techniques and a keen appreciation of product quality are the basic ingredients of Minitube Contents Page Semen collection 04 AndroVision CASA system 05 Semen evaluation 06 07 Semen extenders 08 Transport of chilled semen 09 Insemination 10 Timing of insemination 11 Transcervical insemination 12 Freezing of canine semen 13 14 Automatic freezing 15 Embryo transfer 16 www minitube com 02 WORLD WIDE ACTIVITIES Minitube International AG Phone 49 8709 92290 E Mail minitubeaminitube com Minit b Germany Phone 49 8 09 92290 E Mail minitube minitube de Minitub Ib rica Phone 34977 845 397 E Mail infogminitub iberica com yb HEEE n 06 anii Bh on u 1 4 Pett tt zs 27 3065005 i 6 i HEBE mr eB ae vet Minitube Central Europe 3 iii it ON o Phone 4 421377 877 915 iiU TTE AA E Mail 71 Cn B tt TTC 20000009 HM e ee on de de de de de Baas odd 4444474644 Trta 6664666660566 initub Chi rd Hirten Minitub Chile T SERERE EET 7717 Phone 56 22 653 2 44 mh SAI E dd e E Mail minitubaminitu
3. order to ensure that you are successful in the use of artificial insemination Veterinarians and agro scientists specialised in this field therefore are responsible at Minitube for product development and for consulting with our clients The technical demands of semen processing and preservation also require knowledge and expertise in other fields such as electronics sensorics mechanics chemistry cryobiology and many others At Minitube these demands are met by specially trained engineers and scientists For product development our teams of veterinarians and engineers cooperate closely Together they offer all the knowledge and experience necessary to guarantee the best possible results Minitube International offers you specialised training programs courses and technology transfer In close cooperation with renowned research institutions we conduct fundamental research concerning assisted reproduction technologies as well as applied research that leads to improved techniques in production evaluation and conservation of semen and embryos Originating in Tiefenbach Bavaria Minitube has established 15 competence over many years in more than 100 different countries all over the world With several companies highly trained local representatives and multilingual professionals we keep in contact with the international Al industry Innovative ideas modern production techniques and a keen appreciation of product quality are the basic in
4. 1300 Viability Automated count of the percentage of membrane intact sperm based on a double stain fluorescence assay Module Viability REF 0 Acrosome integrity Automated count of sperm with damaged acrosomes based on a double stain fluorescence assay Module Acrosome integrity REF 0 requires fluorescence microscope AndroVision F E T www minitube com 05 06 SEMEN EVALUATION roscope MBL 2000 Functional microscope for semen analysis with phase achromatic objectives 10x 40x 100x heating system with temperature control unit HT50 with digital temperature display and freely selectable temperature adjustment Binocular REF 12010 2000 With eyepiece video camera and monitor REF 12010 2100 The SDM 1 is a compact highly accurate photometer developed for measuring sperm cell concentrations It can be calibrated for bovine canine equine porcine and small ruminant species Cleaning is simple maintenance is minimal The semen concentration is displayed in million sperm cells ml No dilution of semen samples necessary LED light source provides stable output Cuvette is securely fixed in the correct measuring position Calibration is performed automatically with each sample measurement Photometer SDM 1 calibrated for canine semen REF 12300 0103 Optionally available Multispecies Software for reprogramming to other species REF 12
5. 300 0130 Accessory Microcuvette for SDM 1 100 box REF 12300 1111 www minitube com SEMEN EVALUATION Spermac semen dye for morphological evaluation 4 x 50 ml REF 15405 0000 Farelly stain for morphological evaluation consisting of 3 components 250 ml each REF 15405 0026 Eosin G solution for supravital staining 50 ml REF 15405 0025 Nigrosin solution for supravital staining 50 ml REF 15405 0029 Microscope slide 76 x 26 mm 50 box REF 15400 2400 Cover glass 18 x 18 mm 100 box REF 15401 0990 Immersion oil 8 ml REF 12000 0101 imocytometers Thoma new RE 15150 4771 Neubauer improved REF 15152 4421 B rker REF 15153 0000 Pipettor 10 100 pl REF 12425 4810 Tips for pipettor REF 12425 9409 www minitube com 07 SEMEN EXTENDERS CaniPlus Chill is a culture medium for chilled dog semen The medium is usually completed by adding fresh egg yolk The plain medium can be used without modification for preserving dog semen during up to 3 days CaniPlus Chill with added egg yolk assures high semen quality for up to 8 days Dog semen preserved in CaniPlus Chill is best maintained at 5 C bet 13700000 CaniPlus Chill 20 mi mr 13700 0030 CaniPlus Freeze is a medium for freezing dog semen with the one step method The unique formulation maintains post thaw motility at a high level It contains 376 glycerol as freezing prot
6. 7500 0006 CH6 17500 0026 CH8 rer 8 CH8 er 17500 0028 The Minitube Shunt System for transcervical insemination is a dual purpose endoscopic tool It eliminates vaginal air loss around the Endoscope during insufflation by creating an air tight seal The Shunt System also acts as a stabilising platform for the Endoscope during the procedure REF 17500 0070 www minitube com FREEZING OF CANINE SEMEN Semen straw 0 25 ml clear 2000 bag REF 13407 0010 Semen straw 0 5 ml clear 2500 bag REF 13408 0010 Semen straws made by Minitube are available in many different colours and preprinted upon request Automatic filling and sealing machine for 0 25 and 0 5 ml straws with ultrasound sealer Capacity 4235 straws hour Power supply 110 V 60 Hz or 230 V 50 Hz MPP Uno complete REF 13017 0000 Exchangeable straw hopper REF 13017 0005 Filling nozzle 0 25 ml REF 13017 0012 Suction nozzle 0 25 ml REF 13017 0022 Filling nozzle 0 5 ml REF 13017 0017 Suction nozzle 0 5 ml REF 13017 0027 Washer 5000 bag REF 13021 3000 Filling tubing 100 baq REF 13021 3800 Thermo transfer printer for straws with automatic straw feeder Unmatched clean and clear non abrasive print Prints up to 3 lines per straw even with barcodes logos and specific characters Very simple handling only little maintenance required Suitable for 0 25 ml and 0 5 ml straws REF 13038 0000 Colour band for straw printer Eas
7. bchile cl i i en ded ohh 2 dle de sia dde dle le dde de E m TE 9995 HOO Minitub do Brasil sen 0690409000009 EL 220022222122 Phone 55 51 3222 7876 E IMS ini BHM MPH E Mail minitub minitube com br 2885088000004 8885888 ee 1 9 e eHe H a I me Ec EU Minitube Australia t 444 Phone 61 3 5342 8688 O En 3 EM Me EM E Mail info minitube com au a Een A eb t oe 60994 Sma Minitube Indonesia N Phone 6221 7591 75 96 94 E Mail zulkifliaminitube indonesia com Minitube China Phone 86 10 8240 3857 E Mail info minitube com cn Mexitube Alemania Phone 5244 246 73 46 47 E Mail Minitube India Phone 9111 4309 0668 E Mail info minitube india com Minitube is a registered trademark of Minit b GmbH Germany www minitube com ee S e CC 4 4444 LEE ets T 4 A FF FF PER ELH FFF FF s aA RAT ht ERRARE HR
8. ector Egg yolk is added by the user The convenient het one step freezing protocol allows semen processing at room temperature therefore no additional cooling equipment is required ed at CaniPlus Freeze 20 ml REF 13700 1030 1 7 CaniPlus Enhance is a medium for Al in dogs It is added to frozen thawed chilled or fresh semen prior to insemination especially when semen quality is not optimal It contains sperm motility enhancing ingredients thus improving the breeding performance of the used semen Ti 1 1 CaniPlus Enhance 20 ml REF 13700 0031 Electronic temperature control stainless steel with grid and lid 7 inner dimensions 240 x 210 x 140 mm W x H x D REF 14078 0001 10 inner dimensions 350 x 210 x 140 mm W x H xD REF 14079 0001 Power supply 115 V 60 Hz or 230 V 50 Hz 08 www minitube com TRANSPORT OF CHILLED SEMEN 3 Your benefits A world wide proven system for cooling storage and transport of fresh semen EGUITAINER m The warm semen is automatically and gently cooled down to the ideal storage temperature within 6 to 10 hours The desired temperature is reliably maintained for about 70 hours RUSH BIOLOGICAL MATERIALS Very high level of protection against potential x ray induced damage of the enclosed semen sample FRAGILE Equitainer cup style Complete with cup style isothermaliser sterilis
9. ed plastic cup with lid 2 ballast bags and 2 coolant cans REF 17220 0010 Equitainer tube style Complete with tube style isothermaliser to fit two 50 ml tubes 6 x 50 ml tubes and 2 coolant cans REF 17220 0015 Accessories Coolant can 1 REF 17221 0120 Thermo bag 2 REF 17221 0130 Isothermaliser cup style 3 for 240 ml cup REF 17220 0011 Isothermaliser tube style 4 for two 50 ml tubes REF 17220 0016 Inner plastic cup 5 240 ml graduated with lid REF 17221 0240 50 ml tubes 6 REF 17220 0017 Minitube transport box made of highly insulating Neopor complete with two ice packs two syringes 5 and 10 ml and outer shipping carton holding optimal temperature for appr 45 hours REF 17229 0005 www minitube com INSEMINATION heters for canine vaginal 3 Your benefits e Designed to mimic the natural breeding process Available in three sizes to accommodate a variety of breeds e Stainless steel spine with protective sheath to enhance rigidity e Luer lock injection port with reflux check valve e Inflatable balloon prevents unwanted back flow of semen e Balloon equipped with monitoring pilot balloon and Luer lock check valve CVI Large CH 36 for medium to large sized dogs Length 400 mm Sterilised single use with 10 ml syringe 1 package REF 17500 0035 CVI Medium CH 18 for small and medium sized dogs Length 250 mm Sterilised single use with 5 m
10. gredients of our knowledge and your success We thank you for your interest and your confidence placed in us DR CHRISTIAN SIMMET CEO www minitube com 03 SEMEN COLLECTION Your benefits Allows evaluation of stud dog penis while being collected Colour coded funnels to divide the different ejaculate fractions Dividing the fractions is always useful in preventing unwanted bacteria and contaminations by prostatic fluid Spill proof funnel design prevents accidents during the collection process Non spermicidal materials are easy to clean sterilise and re use Samples immediately ready for analysis centrifugation or extension Components 3 colour coded funnels red blue and white 4 centrifuge tubes 15 ml Canine collection system REF 17500 0203 Alternatively available Semen collection funnel disposable 100 box REF 0 Accessories Semen collection glove extra sensitive sperm safe 100 box Size M REF 11128 0055 Size L REF 11128 0060 Size XL REF 11128 0065 Glove dispenser REF 11128 0010 Hygienic glove for the protection of the collection glove 100 package REF 11128 0100 ReproJelly non spermicidal lubricant water soluble tube with 100 ml resealable REF 11907 0100 Electro ejaculator set consisting of heavy duty carry case with power sup ply unit and connecting cables for external battery Power supply 17 V 100 240 V DC Electro ejaculator REF 11900 0000 E
11. l syringe 1 package REF 17500 0034 CVI Small CH 18 for small sized dogs Length 120 mm Sterilised single use with 5 ml syringe 1 package REF 17500 0032 Rigid plastic pipettes with piece of tubing and Luer adapter Length 22 cm 10 Package REF 17500 0010 Length 22 cm 1 Package REF 17500 0020 Length 44 cm 10 Package REF 17500 0040 Length 44 cm 1 Package REF 17500 0045 Non spermicidal lubricant water soluble Iso osmotic and sperm friendly supports the sperm cell viability Batches are tested for sperm compatibility Ideal viscosity to better coat gloves Al equipment and instruments Tube with 100 ml resealable REF 11907 0100 3 0 canister without dispenser REF 0 Dispenser for canister REF 0 10 www minitube com TIMING OF INSEMINATION ELISA PROGESTERONE TEST Determining the optimal time for insemination is one of the most critical steps for successful reproduction The MiniReader is an accurate reliable and affordable device to read and Applications determine the results of Minitube s ELISA Enzyme Linked Immunosorbent Assay based progesterone test e Determination of the ideal time for artificial insemination Detection of ovulation REF 0 Your benefits The colour change ofthe sample is automatically detected and converted into ng ml Progesterone ELISA test kits contain all reagents and consumables needed to perform the analysis Performing the ELISA i
12. lectro ejaculator probe 3 8 REF 11900 0509 Electro ejaculator probe 1 2 REF 11900 0510 04 www minitube com ANDROVISION MORE THAN CASA n functionality analysis This highly efficient automated system for computerised semen analysis stands out for the integration of the classic CASA analysis with more advanced assays for sperm functionality Concentration and motility as well as sperm integrity and viability can be assessed accurately and quickly with up to 1000 cells per field making AndroVision the perfect tool for the objective and complete semen evaluation All images and data are stored for later review An analysis report can be generated as a hard copy print or a digitised file These features and more make AndroVision the ideal choice for canine semen assessment in a research and production lab AndroVision software with PC and accessories REF 12500 0000 AndroVision camera high speed colour camera REF 12500 4200 Morphology and morphometry Interactive system for analysis of sperm morphology and morphometry Identifies sperm of stained and unstained samples and measures length and width of the sperm head head shape and midpiece asymmetry of each single sperm cell acc to Kr ger Results can be classified into a large range of morphologic abnormalities The classification according to morphological criteria is a self learning process Module Morphology and morphometry REF 12500
13. ng rates 0 01 to 60 C min Heating rates 0 01 to 15 C min Display accuracy 0 01 C Temperature measurement via 2 Pt 100 resistance thermometers Capacity approx 900 straws per freezing cycle Software for freeze program and control unlimited storage of programs and processed files colour graphic display of all temperature curves wide possibility of parameter settings Dimensions 540 x 420 x 520 mm Ix w x h 29 kg IceCube 145 with 12 touch screen monitor and connector kit to be con nected to computer and LN pressure container REF 16821 2000 IceCube 145 without monitor with connector kit to be connected to computer and LN pressure container REF 16821 1000 Accessories Rack for IceCube 145 for 36 straws REF 16821 5036 Handling rack for IceCube 145 REF 16821 5030 Loading block for rack 16821 5036 REF 16821 5037 Spacer for IceCube REF 16821 5018 Liquid Nitrogen pressure container for IceCube 14S 60 REF 16820 1900 Roller base for LN container REF 16501 0204 www minitube com 15 16 EMBRYO TRANSFER Micropipettor for handling of embryos Easy and precise pipetting by handling wheel for all sizes of capillary tubes up to 1 TypeA REF 19022 0000 Type B REF 19022 0002 Glass capillary tube 250 package 10 ul REF 19022 0010 20 yl REF 19022 0020 50 pl REF 19022 0050 Steripette for embryo handling 55 mm length sterilised 50 package REF 19025 0050 Disposable s
14. s straight forward with or without the means of the PipetButler Manual pipetting of reagents is not needed when the PipetButler is used The Progesterone concentration is based on a standard curve derived from three standards The PipetButler automates sample preparation for the ELISA test and facilitates routine work in the lab The test kit reagents are supplied individually and loaded easily into the PipetButler The ELISA reagents are automatically added to the serum sample REF 0 34 Your benefits The PipetButler is equipped with a heated well tray set to 37 C which is the optimum temperature for ELISA reactions Automatic addition of the reagents avoids handling errors A light at the PipetButler indicates completion of processing steps Test kits Test kit for measuring progesterone in canine serum for 10 samples for manual pipetting REF 21900 1110 Test kit for measuring progesterone in canine serum for 10 samples for use with PipetButler REF 21900 1210 Shorr staining solution for examining presence of cornification in epithelial cells ofthe female dog 500 ml REF 15405 0080 www minitube com 1 12 TRANSCERVICAL INSEMINATION Transcervical insemination is none invasive not painful and typically performed without sedation e Angular eye piece autoclavable ntegrated bilateral fibre glass cord 1 Your benefits e 2channel air insufflation flushing side connec
15. tions Working channel CH6 for use with instruments up to CH5 43cm long e Working channel enables insertion of instruments under direct visuali CH9 5 distal tip CH8 sation for insemination biopsy and flushing Allows easy access to the cervix and uterus in dogs of all sizes REF 23700 1510 Minitube has configured a complete system for performing transcervical insemination utilising industry leader Karl Storz endoscopic components This system in conjunction with the Endoscope and TC Cannulas provides the perfect combination of tools to effectively and efficiently perform transcervical insemination on all sizes of canines Cart system not included The Tower System includes Veterinary Ill Camera REF 23700 1513 Vet halogen cold light fountain 150 W REF 23700 1512 Fiber optic light guide cable REF 4 LCD high resolution colour monitor REF 18000 0189 e Clear markings every centimetre with additional indicators every 5 cm Supple material reinforced by a removable stylet for easy insertion e Adapter for semen containing syringe included e Single packed length 70 cm TC cannulas with single port TC cannulas with dual port The eye is 0 5 cm from tip Has an additional eye approx 2 cm to prevent blockage from tip and rotated 60 For Minitube Endoscope and others CHA er 17500 0004 CH4 REF 17500 0024 CHS REF 17500 0005 CH5 rer 17500 0025 For endoscopes from other companies CH6 rer 1
16. yCoder Black roll with 200 m REF 13038 0010 Gold roll with 300 m REF 13038 0012 Red roll with 300 m REF 13038 0015 Silver roll with 300 m REF 13038 0017 www minitube com 3 FREEZING OF CANINE SEMEN Technical details Simple but effective and robust standardisation of vapour freezing Floating rack for straws included The distance between straws and liquid nitrogen level can be adjusted with floating rack Fits 0 5 ml and 0 25 ml straws With high quality styrofoam chest and stainless steel inner box with working tray Transportable For 20 straws REF 15043 0636 For 90 straws REF 15043 0736 Accessories Loading block for 90 straws rack REF 15043 0738 Additional racks for freezing unit for 20 straws REF 5015043 0600 for freezing unit for 90 straws REF 5015043 0900 Short and long triangular cassettes for 10 20 straws 0 5 ml or 28 56 straws 0 25 ml REF 16981 xxxx Short and long flat cassettes for 3 6 straws 0 5 ml or 6 12 straws 0 25 ml REF 16980 XXXX Single use shipper ensures a safe shipment of temperature sensitive semen doses IATA conform Static holding time 4 days Capacity 200 x 0 25 100 x 0 5 ml straws 3 liter Weight 4 5 kg REF 16502 0003 14 www minitube com AUTOMATIC FREEZING Computer controlled freezer with comfortable data input and automatic recording of the freezing curve Technical features Temperature range 40 to 180 C Freezi
17. yringe 1 ml luer end sterilised REF 19025 0000 Embryo culture dish with lid sterilised 5 wells rounded well edges shallow wells for easy handling 4 bag REF 19021 0001 Embryo transport vial 5 ml with snap cap and round bottom steri lised single packed 500 package REF 19180 5006 Pen for marking very fine alcohol permanent without illustration Black REF 19045 0100 Manual sealer for straws securely seals all kinds of straws by applying heat at the end of the straws Also seals in liquid nitrogen vapour Consists of a plier welding head adapted for straws and a control unit for varying heat level REF 13135 2500 Vitrification unit Set for vitrification of embryos or oocytes in OPS straws or Ministraws Styrofoam box with stainless steel vitrification bath material for vitrification DVD with instructions for working with OPS straws REF 19500 2000 www minitube com Minitube is an ISO 9001 2008 certified company Y WTIWIS SNOLLVHISQTII 031432X3 500483 SNOILVDISIGOW OL 5 i www minitube com minitu be SLOZ EO OLS0 00008 33
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