procise/procise clc protein sequencer
Contents
1. 2 3232. Applied Biosystems Je dal Input Output Connections Location The exact locations of the input output connections on the PROCISE PROCISE cLC Protein Sequencer are shown in Figure 2 1 The input outp
3. OFF ON OFF
4. AU DAMA TIA BH EE JVDRIE o FR ES Sd PE E F HJ Nf o gt gt l O JFERR SE MI AB ZH EMMA eare IEE RN ER AU terra za gt jun BER p 662548 o CDE gt gt Symboles des alertes de s curit Symboles lectriques Le tableau suivant donne la signification de tous les symboles lectriques qui figurent sur les appareils Applied Biosystems En pr sence de l un de ces symboles il est imp ratif de se conformer aux consignes de s curit appropri es Position MARCHE de l interrupteur d alimentation principal Position ARR T de l interrupteur d alimentation principal Positions MARCHE ARR T de l interrupteur d alimentation principal bouton poussoir Borne pouvant tre reli e la mise la terre d un autre appareil Ce n est pas une borne de mise la terre prot g e Borne de mise la terre de protection devant tre reli e la terre avant d effectuer tout autre raccordement lectrique l appareil Borne recevant ou fournissant une tension ou un courant de type alternatif Borne pouvant recevoir ou fournir une tension ou un courant de types alternatif et continu ATTENTION Indique la pr sence d une haute tension et avertit l utilisateur de proc der avec pr caution ATTENTION Avertit utilisateur de la n cessit de consulter le manuel pour obten
5. npenynpexaer 3 0 6 o 6 1 6 1 OCTOPOXKHOCTE HPEJOCTEPE KEHHIE npegnaraeTt 6 3a ganbHenwuel uHpopmaumen K PyKOBOACTBy n npeaynpexgaer o 6 1 6 OCTOPOXKHOCTE PP O Huxe npegcraBneH unrniocrpupoBaHHbll 006 0 BCeX CUMBONOB KOTOPbIE 70 3 010 B CHMBOJIbI puoopax Applied Biosystem instruments n nMeror 01 0 K DE3OMACHOCTN He CBA3AHHON C SNEKTPUMECTBOM IIPE IOCTEPE2KEHME cBugeTenbeTByeT O onacHoctu oxora Bo n36exaHme oxora 0 6 NpoABNAUTE OCTOPOKHOCTE pa6ore Ha AaHHbIX ydacrkax OTOT CUMBON CBUACTENbCTBYET o 0 1018 B npuGope nasepa gt gt S mbolos de alerta de seguridad S mbolos el ctricos En la siguiente tabla se muestra un glosario ilustrado de todos los s mbolos el ctricos que se utilizan en los instrum
6. 1 nonoxeHune 6 CETEBOTO 5 OTOT CUMBON 03 YTO KNemma MOXer ObiTb NOACOeANHEHA K TOUKE 0 HyneBo ana apyroro npubopa Ita He ABNAETCA KJIeMMO 3 6 3A3eMNMEHUA OTOT CUMBON 03 YTO knemma ABNAeTCA KIIEMMON 3allINTHOTO 3a3eMMeHUA koropas nomkHa bITb rioncoenuHeHa K 3alLIATHOMY 3A3EMIIEHNIO paHbllie YEM HAUHETCA K npubopy Apyrux anekrpuueckux COCAUHEHUU KneMMa oTMeueHHas STUM CUMBOJIOM ABIIAETCH NNN 10 Hanpaxenna KneMMa oTMeueHHas STUM CUMBOJIOM ABIIAETCH NPUEMHUKOM NNN 10 NepeMmeHHoro YM MOCTOAHHOTO TOKa NNN 6 CBUACTENECTBYET o BbICOKOTO
7. 09 1 1 Technical Support 3 1 7 1 2 To Re ch Us On the Wei Sied ds ua o an 1 1 2 To Contact Technical Support by E Mail 1 2 Hours for Telephone Technical Support 1 2 To Contact Technical Support by Telephone or Fax 1 2 To Reach Technical Support Through the Internet 1 5 To Obtain Documents on Demand 1 5 Premstallation Checklist 1 6 bouts Checklist vea 3 o 11 0 1 6 1 3 1 1 6 Items Shipped with this Instrument 1 6 11 3 dadie 1 6 Hazardous Chemicals Warning 1 6 Installation Chemical Mit era 1 1 0 1 9 Items Needed but Not Supplied 2 ee 1 10 ROSAD cic Gaiters Gis et aro Oe et iba bau es dai dci dune 1 10 VV ALOE 3 7 1 7 2 1 10 Edguipment and S UpplieSs 1 1 10 Laboratory Saleen 3 3333 Go te 1 11 Onsite Representative Lac coro ace es Be ae ds een 1 11 Re gured Safe Equipment sao dar msn tuer 1 11 I abordtoby Space Required 200d 3 1 12 ons RA 1 12 Location or I
8. PROCISE PROCISE CLC PROTEIN SEQUENCER Site Preparation and Safety Guide AS 5 ems O Copyright 2002 Applied Biosystems For Research Use Only Not for use in diagnostic procedures ABI PRISM the ABI PRISM design Applied Biosystems Aquapore AmpliCover Anitron Biobytes Brownlee FastPhoramidite GeneScan Genotyper HLP INHERIT Masterpiece MicroAmp MicroCoat MPLC NEWGUARD ONESTEP OPC PCR MATE Phosphalink POLYPORE Precipitette ProBlott PROCISE PROCISE cLC ProFocus ProSort ProSpin SeqEd Sequence Navigator SPHERIS SPHERI10 StockMarks Stretch Synergy SynthAssist and VeloSep are registered trademarks of Applera Corporation or its subsidiaries in the U S and certain other countries ABI AmpF STR AutoAssembler BaseSprinter CATALYST GeneAssist LV40 MatchMaker PDQ Primer Express and ProSorb are trademarks of Applera Corporation or its subsidiaries in the U S and certain other countries All other trademarks are the sole property of their respective owners Contents I Site Preparation Before ou lala Rice eode eR do Ani ee eade Bateau fio ie Ua ral ANA 1 1 Promstallatione nina 7 1 1 111 909 1 1 Operator Trans solu e ane 11 1 9 1 1 RS I SR A I bt dae Thus 0690 1 1 yz uku a ayaka 11111111 1 11 5 1 1 od e rod 1 76 1 11111110
9. government regulations common scientific terminology units of measurement and chemicals The following tables contain lists of acronyms and abbreviations commonly used in Waste Profiles and MSDSs Acronyms and abbreviations for organizations government regulations and scientific terminology Table 1 1 Acronyms and abbreviations Term Explanation ACGIH American Conference of Governmental Industrial Hygienists CAS Chemical Abstract Service Reference Number for Specific Pure Chemical 00 Closed cup testing of flash point CFR Code of Federal Regulations Regulations published by the United States Government CERCLA Comprehensive Environmental Response Compensation and Liability Act Superfund is a federal las administered by EPA DFG MAK Federal Republic of Germany s Maximum Contamination Value in the workplace similar to PEL in the USA DOT United States Department of Transportation regulates transportation of hazardous material USA EPA United States Environmental Protection Agency regulates use disposal or emission of hazardous material USA IDLH Immediate Danger to Life and Health LC o Lowest published lethal concentration Cao Lethal concentration in air that kills 5096 of a specified population LDso Lethal dose that kills 50 of a specified population LEL Lower Explosion Limit MSHA Mine Safety and Health Administration recommends respirators NFPA National Fire Protection Associatio
10. nose throat and lungs Higher concentrations may cause pulmonary edema unconsciousness and death EYES May cause severe irritation or burns EMERGENCY AND FIRST AID PROCEDURES If conscious give large quantities of water immediately and induce vomiting Get medical attention immediately Do not induce vomiting if unconscious SKIN Remove contaminated clothing Flush the contaminated area with water and wash with mild soap or detergent Get medical attention INHALATION Provide fresh air and rest If breathing is difficult provide oxygen and get medical attention immediately EYES Flush eyes immediately with large amounts of water for at least 15 minutes Get medical attention Section 6 REACTIVITY DATA STABILITY Stable INCOMPATIBILITY Contact with strong oxidizing agents or concentrated acids or bases may cause fire or explosion HAZARDOUS COMBUSTION OR DECOMPOSITION PRODUCTS Burning may release toxic vapors and gases including phosgene hydrogen chloride hydrogen fluoride carbon monoxide and oxides of nitrogen HAZARDOUS POLYMERIZATION Will not occur Chemical Safety 3 7 Section 7 SPILL OR LEAK PROCEDURES STEPS TO BE TAKEN WASTE DISPOSAL Avoid inhalation and skin contact Wear protective clothing Ventilate area of spill or leak Remove all ignition sources Small quantities may be collected with absorbent
11. 1 650 638 5981 then press 21 Fluorescent DNA Sequencing 1 800 831 6844 1 650 638 5981 then press 22 Fluorescent Fragment Analysis includes 1 800 831 6844 1 650 638 5981 GeneScan applications then press 23 Product or Product Area Telephone Dial Fax Dial Integrated Thermal Cyclers ABI PRISM9 877 and Catalyst 800 instruments 1 800 831 6844 then press 24 1 650 638 5981 ABI PRISM 3100 Genetic Analyzer 1 800 831 6844 then press 26 1 650 638 5981 Biolnformatics includes BioLIMS BioMerge and SQL GT applications 1 800 831 6844 then press 25 1 505 982 7690 Peptide Synthesis 433 and 43X Systems 1 800 831 6844 then press 31 1 650 638 5981 Protein Sequencing Procise Protein Sequencing Systems 1 800 831 6844 then press 32 1 650 638 5981 PCR and Sequence Detection 1 800 762 4001 then press 1 for PCR 2 for the 7700 or 5700 6 for the 6700 or dial 1 800 831 6844 then press 5 1 240 453 4613 Voyager MALDI TOF Biospectrometry and Mariner ESI TOF Mass Spectrometry Workstations 1 800 899 5858 then press 13 1 508 383 7855 Biochromatography BioCAD Workstations and Poros Perfusion Chromatography Products 1 800 899 5858 then press 14 1 508 383 7855 Expedite Nucleic acid Synthesis Systems 1 800 899 5858 then press 15 1 508 383 7855 Peptide Synthesis Pioneer and 9050 P
12. 3 Siorme Hazardous Waste 7 1111114 1 8909 3 3 Instrument Waste Profile OVervIe Ww susa 1 1 1 1 1 a 3 4 General Inforrbat olus 1111111111895 3 4 Waste Broule SCCHONS doses gs bidielle 3 4 Material Safety Data Sheets 3 30 3 0 S ona mc pr PLI 3 0 Contents OF MSDSS Aus es 11 900 3 0 How to Get Extra Copies of MSDSS esses pise A aS 3 0 MSDSs for the PROCISE cLC Protein Sequencer 3 10 MSDSs for Applied Biosystems Chemicals 3 10 MSDSs for Chemicals Not Supplied by Applied Biosystems 3 10 A Acronyms and Abbreviations Acronyms and Abbreviations Used in Waste Profiles and MSDSs A 1 Introduce HOD TT A 1 Organizations Regulations and Scientific Terminology A 1 Units of Measurement ropa ida A 2 Chemicals si tol ue br ee ease E RR d e Medo een dg dot oa A 3 iii Site Preparation Before You Start Preinstallation Operator Training Performance Verification Ordering Supplies Unpacking Before the instrument is installed the installation site must be prepared so that the instrument can operate correctly and safely Careful attention to the requirements presented here will simplify the installation procedure Training of operators
13. 760 mm N A SPECIFIC GRAVITY H20 1 1 01 VOLATILITY vol 98 99 APPEARANCE AND ODOR Colorless two phase liquid with a fruity vinegar odor FREEZING POINT N A pH RANGE 0 2 bottom phase SOLUBILITY IN H20 Top 5 btm 100 Section 4 fire and explosion hazard data Acetonitrile data only FLASH POINT Closed Cup Ethyl Acetate only 5 C 23 F FLAMMABLE LIMITS Ethyl Acetate only 2 2 LEL 11 UEL FIRE EXTINGUISHING MEDIA Dry chemical carbon dioxide or Halon alcohol foam SPECIAL FIRE FIGHTING PROCEDURES Use self contained breathing apparatus and protective clothing to prevent skin and eye contact Section 5 HEALTH HAZARD DATA EXPOSURE LIMITS See Section II For Ethyl Acetate the IDLH level is 10 000 ppm For Heptane NIOSH recommends a TWA of 85 ppm and a ceiling level of 440 ppm The STEL is 500 ppm and the IDLH level is 4250 ppm For Acetonitrile the STEL is 60 ppm and the IDLH level is 4 000 EFFECTS OF ACUTE OVEREXPOSURE SWALLOWING Harmful if swallowed Causes severe irritation of eyes nose and throat Higher concentrations may cause liver and kidney damage unconsciousness and death SKIN May cause severe irritation or burns Allergic Skin sensitization may also occur 3 6 Chemical Safety EXPOSURE LIMITS See Section II INHALATION May cause irritation of eyes
14. Index link for the document type you want then find the document you want and record the index number c Use the index number when requesting documents following the procedures below by phone for fax a From the U S or Canada call 1 800 487 6809 or delivery from outside the U S and Canada call 1 858 712 0317 b Follow the voice instructions to order the documents you want Note There is a limit of five documents per request through the a Access the Applied Biosystems Technical Support Web site at Internet for fax or http www appliedbiosystems com techsupp e mail delivery b Under Resource Libraries click the type of document you want c Enter or select the requested information in the displayed form then click Search d Inthe displayed search results select a check box for the method of delivery for each document that matches your criteria then click Deliver Selected Documents Now or click the PDF icon for the document to download it immediately e Fill in the information form if you have not previously done so then click Deliver Selected Documents Now to submit your order Note There is a limit of five documents per request for fax delivery but no limit on the number of documents you can order for e mail delivery Site Preparation 1 5 Preinstallation Checklist About this Checklist Use this checklist to ensure that all preparations have been made for installation A service re
15. Mexico 305 670 4350 305 670 4349 To Reach Technical We strongly encourage you to visit our Web site for answers to frequently asked Support Through questions and for more information about our products You can also order technical the Internet documents or an index of available documents and have them faxed or e mailed to you through our site The Applied Biosystems Web site address is http www appliedbiosystems com techsupp To submit technical questions from North America or Europe Step Action 1 Access the Applied Biosystems Technical Support Web site 2 Under the Troubleshooting heading click Support Request Forms then select the relevant support region for the product area of interest 3 Enter the requested information and your question in the displayed form then click Ask Us RIGHT NOW blue button with yellow text 4 Enter the required information in the next form if you have not already done so then click Ask Us RIGHT NOW You will receive an e mail reply to your question from one of our technical experts within 24 to 48 hours To Obtain Free 24 hour access to Applied Biosystems technical documents including MSDSs Documents on is available by fax or e mail or by download from our Web site Demand To order documents Then by index number a Access the Applied Biosystems Technical Support Web site at http www appliedbiosystems com techsupp b Click the
16. Units of The following are abbreviations for units of measurement Table 1 2 Abbreviations for units of measurement Abbrev Unit of Measurement number C degrees Celsius F degrees Fahrenheit uL microliter um micron umol micromole AUFS absorbency units full scale ft foot i d inside diameter in inch L liter m meter A 2 Acronyms and Abbreviations Table 1 2 Abbreviations for units of measurement Abbrev Unit of Measurement mg milligram mL milliliter mm millimeter o d outside diameter P N Applied Biosystems part number psi pounds per square inch sec second V volt Chemicals The following are abbreviations for chemicals Table 1 3 Abbreviations for chemicals Abbrev Definition A adenine AA amino acid 1Ac acetyl Acl acetylimidazole Acm acetamidomethyl ACO acetic anhydride ACN acetonitrile ACT activator vessel BHA resin benzhydrylamine resin t Boc tert butyloxycarbonyl Bxl benzyl Br Z 2 bromobenzyloxcarbonyl t Bu tert butyl C cytosine CHO formyl CH3Bzl 4 methylbenzyl CHSOBZI 4 methoxybenzyl CI Z 2 chlorobenzyloxycarbonyl CPG Controlled Pore Glass DCA dichloroacetic acid DCC dicyclohexylcarbodiimide DCM dichloromethane DCU dicyclohexylurea DIEA diisopropylethylamine DMAP 4 dimethylaminopyridine
17. WARNING Warns the user that serious physical injury or death to the user or other persons could result if these precautions are not taken Instrument Labeling Safety Labels Signal Words oafety labels are located on the instrument Each label consists of a Signal Word panel and a Message Panel A Safety Alert Symbol indicates a potential personal safety hazard If multiple hazards exist the signal word corresponding to the greatest hazard is used DANGER indicates an imminently hazardous situation that if not avoided will result in death or serious injury most extreme CAUTION indicates a potentially hazardous situation that could result in minor or moderate injury to the user or damage to the instrument WARNING indicates a potentially hazardous situation that could result in death or serious injury Instrument Safety 2 1 Labels That May Be The following Danger Caution and Warning labels listed in English and French Found On Your below may be found on your instrument Instrument 2 2 Instrument Safety English French CAUTION Hazardous chemicals Read the Material Safety Data Sheets before handling Attention Produits chimiques dangeureux Lire les fiches techniques de s rete de materiels avant la manipulation des produits CAUTION Hazardous waste Read the Waste Profile before handling or disposal Attention Dechets dangeureux Lire les renseignements sur les dechets avant de manip
18. caused by other devices using the same power source refrigerators air conditioners and centrifuges or by outside influences such as lightning A dedicated line and ground between the instrument and building main electrical service are necessary to prevent such problems If your environment contains devices that are electrically noisy or you are in an area with frequent electrical storms a line conditioner with a recommended capacity of 2 0 kVA will enhance the reliability of your system This may be lower depending on the conditioner or power supply design continued on next page Power Outages The instrument has been designed to recover from short periods of power outage loss and continue operation provided that the line voltage does not become excessively noisy before the outage If increased protection during a power outage is desired you may want to install an uninterruptible power supply UPS We recommend a capacity of 2 0 kVA The UPS will involve a higher cost than a line conditioner Site Preparation 1 15 Laboratory Environmental Requirements Altitude Temperature and Humidity Pollution Heat Emission Immunity Statement 6 1 16 Site Preparation This instrument is for indoor use only and for altitudes not exceeding 2 000 meters 6 500 ft above sea level The laboratory temperature should be maintained between 15 30 C 59 85 F The instrument can tolerate up to 8096 relative humidity Avoid placin
19. the instrument itself This will protect you from liability if any damage occurred during shipping IMPORTANT You must unpack the Installation Chemical Kit and store the components as specified in Table 1 1 WARNING Some chemicals used with Applied Biosystems instruments are hazardous and can cause injury illness or death Always read the appropriate Waste Profiles and MSDSs before interacting with the instrument and chemicals in any way Hazardous Chemical Warnings are prominently displayed on the labels of all hazardous materials Before unpacking the Installation Chemical Kit storing chemicals or interacting with the chemicals and instrument read Chapter 3 Chemical Safety of this manual Chapter 3 contains the Waste Profiles and Material Safety Data Sheets MSDSs that pertain to this instrument continued on next page Installation Chemical Kit Unpack the Installation Chemical Kit upon receipt The Installation Chemical Kit is intended to be used during installation to verify instrument performance Store the chemicals and reagents as indicated in the table below Note All reagents are common to both kits below P N 401690 and P N 402064 unless otherwise indicated Table 1 1 PROCISE PROCISE cLC Protein Sequencer Installation Chemical Kit Components P N 401690 and P N 402064 Storage P N Description Conditions 400208 H1 596 PITC in N Heptane 20 C 401535 R2B n Methylpiperidine in methanol and water 4 C 4
20. within 2 5 m 8 ft of the instrument rear panel The following table specifies the electrical operating range for various parts of the world Location Volts AC Frequency Japan 100 x 10 50 60 Hz x 196 USA Canada 120 x 1096 50 60 Hz x 196 Europe pre 1992 220 x 1096 50 60 Hz x 1 EC 230 1096 50 60 Hz x 196 UK pre 1992 240 6 10 50 60 Hz x 1 Australia 240 6 10 50 60 Hz x 1 Certain types of electrical noise are greatly exaggerated by poor or improper electrical ground connections To prevent these problems it is very important to have a dedicated line and ground between the instrument and building main electrical service Inthe USA Canada and Japan the instrument is supplied with a detachable cord equipped with a standard three prong plug In Europe and Australia the instrument is supplied with an detachable electrical cord equipped with a standard EC plug The Macintosh computer can be plugged into any standard electrical receptacle after it has been configured for the proper voltage Line voltage must be within 10 of the nominal value High or low voltages may have adverse effects on the electronic components of the instrument In areas where the supplied power is subject to fluctuations exceeding these limits a power line regulator may be required Short duration high voltage spikes often cause random failures in microprocessor controlled instrumentation These spikes can be
21. 00003 R3 Trifluoroacetic Acid Room temp 400028 RAA 25 Trifluoroacetic Acid in water 4 C 400315 R5 Acetonitrile with DTT Room temp 400079 S1 N Heptane Procise cLC only Room temp 400854 S2B Ethyl Acetate Room temp A00008 S3 N Butyl Chloride Room temp 400314 S4B 20 Acetonitrile in water Procise only Room temp 400470 MeOH Methanol Room temp 400879 PTH Analyzer Standards 20 C 400385 Biobrene Plus 4 C 400979 p Lactoglobulin 4 C 401446 Premix buffer concentrate 4 C 402051 SAC 10 Acetonitrile in water 1 L Procise Hoom temp 401464 Solvent A3 3 5 THF Tetrahydrofuran in Water 1 L Procise Room temp 401570 Solvent B2 1296 Isopropanol and Acetonitrile 1 L Procise Room temp 401887 Solvent A3 3 5 THF Tetrahydrofuran in Water 450 mL cLC Room temp 401886 Solvent B2 1296 Isopropanol and Acetonitrile 450 mL cLC Room temp Note For the Procise sequencer solvents will be in 1 L bottles For the cLC sequencer solvents will be in 450 mL bottles Site Preparation 1 9 Items Needed but Not Supplied Resupply The Installation Chemical Kit components are completely consumed during the installation and initial testing of the instrument To ensure an uninterrupted supply of reagents and eliminate the higher transportation costs of rush shipments you should order additional chemicals and supplies in advance Water Equipment and Supplies 1 10 Site Preparation Although the Installation Chemical Kit contains all the reagent
22. 14 S4B 20 acetonitrile in water P N 901829 Solvent A3 3 5 THF in water P N 902209 Solvent B2 acetonitrile 2 Propanol P N 902675 SAC 10 Acetonitrile in water P N 901829 MSDSs for For chemicals required for this instrument but not manufactured or sold by Applied Chemicals Not Biosystems obtain the MSDSs from their manufacturers Brief warnings for these Supplied by Applied chemicals are listed below Biosystems WARNING CHEMICAL HAZARD Acetone is a flammable chemical irritating to respiratory system if inhaled It can cause liver kidney and eye damage Wear protective clothing chemical resistant gloves and protective eyewear WARNING CHEMICAL HAZARD Methanol is a flammable chemical irritating to respiratory system if inhaled It can cause liver kidney and eye damage Wear protective clothing chemical resistant gloves and protective eyewear WARNING CHEMICAL HAZARD Sodium Dodecyl Sulfate SDS is a toxic chemical that is harmful to lungs and internal organs if swallowed Contact with eyes may cause serious damage Wear protective clothing chemical resistant gloves and protective eyewear Chemical Safety 3 11 Acronyms and Abbreviations Acronyms and Abbreviations Used in Waste Profiles and MSDSs Introduction Organizations Regulations and Scientific Terminology Waste Profiles and MSDSs use acronyms and abbreviations for certain organizations
23. 43 0 1 867 35 75 0 43 0 1 867 35 75 11 Belgium 32 0 2 712 5555 32 0 2 712 5516 Czech Republic and Slovakia Praha 420 2 61 222 164 420 2 61 222 168 Denmark Naerum 45 45 58 60 00 45 45 58 60 01 Finland Espoo 358 0 9 251 24 250 358 0 9 251 24 243 France Paris 33 0 1 69 59 85 85 33 0 1 69 59 85 00 Germany Weiterstadt 49 0 6150 1010 49 0 6150 101 101 Hungary Budapest 36 0 1 270 8398 36 0 1 270 8288 Italy Milano 39 0 39 83891 39 0 39 838 9492 Norway Oslo 47 23 12 06 05 47 23 12 05 75 Poland Lithuania Latvia and Estonia 48 22 866 40 10 48 22 866 40 20 Warszawa Portugal Lisboa 351 0 22 605 33 14 351 0 22 605 33 15 Russia Moskva 7 095 935 8888 7 095 564 8787 South East Europe Zagreb Croatia 385 1 34 91 927 385 1 34 91 840 Spain Tres Cantos 34 0 91 806 1210 34 0 91 806 1206 Sweden Stockholm 46 0 8 619 4400 46 0 8 619 4401 Switzerland Rotkreuz 41 0 41 799 7777 41 0 41 790 0676 The Netherlands Nieuwerkerk a d IJssel 31 0 180 331400 31 0 180 331409 United Kingdom Warrington Cheshire 44 0 1925 825650 44 0 1925 282502 All other countries not listed 44 0 1925 282481 44 0 1925 282509 Warrington UK Japan Japan Hacchobori Chuo Ku Tokyo 81 3 5566 6230 81 3 5566 6507 Latin America Del A Obregon
24. DMF dimethylformamide DMSO dimethylsulfoxide DNA deoxyribonucleic acid Acronyms and Abbreviations A 3 Table 1 3 Abbreviations for chemicals continued Abbrev Definition Dnp 2 4 dinitrophenyl Et ethyl EtOH ethanol Fmoc 9 fluorenylmethyloxycarbonyl G guanine HBTU 2 1 H benzotriazol 1 yl 1 1 3 3 tetramethyl uronium hexafluorophosphate HLP high loaded polystyrene HMP resin p hydroxymethylphenoxymethyl polystyrene resin HOAc acetic acid mBHA resin 4 methylbenzhydrylamine resin MeOH methanol Mob 4 methoxybenzyl Mtr 4 methoxy 2 3 6 trimethyl benzene sulfonyl Mts mesitylene 2 sulfonyl NMI 1 Methylimidazole NMP N Methylpyrrolidone N methyl 2 pyrrolidone OBt ethyl ester OMe methyl ester PAM resin phenylacetamidomethyl resin PEG polyethylene glycol RV reaction vessel SSPS solid phase peptide synthesis T thymine TETD tetraethylthiuram disulfide TFA trifluoroacetic acid TFMSA trifluoromethane sulfonic acid THF tetrahydrofuran Tos 4 toluenesulfonyl tosyl Tri trityl U uracil Z benzyloxcarbonyl A 4 Acronyms and Abbreviations Headquarters 850 Lincoln Centre Drive Foster City CA 94404 USA Phone 1 650 638 5800 Toll Free 1 800 345 5224 Fax 1 650 638 5884 Worldwide Sales Offices Applied Biosystems vast distribution and service network composed of highly trained support and appl
25. DSs before handling or disposing of waste The Instrument Waste Profile is NOT a substitute for MSDS information See specific MSDS sheets in this chapter for chemical constituent health Waste Profile Information in the Instrument Waste Profiles is divided into ten sections The names of Sections the ten sections are listed below toxicological and hazard information For a complete list of acronyms and abbreviations used in Waste Profiles see Appendix A Topic Section Identification 1 Approximate Composition Physical Data Fire and Explosion Hazard Data Health Hazard Data Heactivity Data Spill or Leak Procedures Special Protective Equipment oO oco D Special Precautions O Additional Information 3 4 Chemical Safety PROCISE PROCISE CLC Protein Sequencing System Waste Profile Emergency Phone Numbers USA section 1 IDENTIFICATION 650 570 6667 UK 0925 825650 The liquid waste from PROCISE cLC is collected in a one liter rectangular glass waste bottle located behind the right hand door of the instrument at the lower right A spring loaded aluminum cap holds the bottle in place The cap is simply lifted up to remove the waste bottle This waste is a complex mixture of reagents and solvents which may have properties of greater hazard than the individual components by themselves The waste consists of two phases The top non aqueous phase is highly f
26. H OA ASS WE CH RO OI 2 OM PILHA S mbolos de alerta de seguranca S mbolos el tricos A tabela a seguir constitui um gloss rio ilustrado de todos os s mbolos el tricos usados nos instrumentos Applied Biosystems Sempre que um desses s mbolos aparecer num instrumento siga os procedimentos adequados de seguranca Este s mbolo indica que o interruptor de energia el trica est na posic o ligado Este s mbolo indica que o interruptor de energia el trica est na posic o desligado Este s mbolo indica a posic o ligado desligado de um interruptor principal de energia el trica do tipo bot o de press o Este s mbolo indica que um terminal pode estar conectado a uma refer ncia de aterramento de sinal de um outro instrumento Este n o um terminal terra protegido Este s mbolo indica que este 6 um terminal de aterramento de protec o que deve ser ligado terra antes de se fazer qualquer outra liga o el trica ao instrumento Um terminal marcado com este s mbolo recebe ou transmite tens o ou corrente alternada Um terminal marcado com este s mbolo recebe ou fornece tens o ou corrente alternada ou cont nua CUIDADO Este s mbolo indica a presenca de alta tens o e avisa o usu rio para proceder com cuidado CUIDADO Este s mbolo serve como alerta para que se consulte o manual a fim de se o
27. L WASTE HAZARD Waste produced by this instrument is potentially hazardous and can cause physical injury illness or death Dispose of the contents of the waste tray and waste bottle in accordance with all applicable health and environmental laws and regulations The following are important requirements for handling hazardous waste Ensure that the waste container is correctly installed Always handle hazardous materials beneath a fume hood that is connected in accordance with all installation requirements During transporting ensure that waste containers are tightly sealed with the cap provided Read the Instrument Waste Profiles in this chapter before handling or disposing of hazardous waste Read all applicable Material Safety Data Sheets before handling or disposing of hazardous waste Dispose of hazardous waste in accordance with all applicable laws and regulations WARNING Never collect or store waste in a glass container because of the risk of breaking or shattering The following are guidelines for storing hazardous waste Do not store waste for long periods of time Donotstore large amounts of waste in the lab Do not store waste in direct sunlight or heat on or off instrument Chemical Safety 3 3 Instrument Waste Profile Overview General Information The Instrument Waste Profile provides essential information about hazardous waste Read the Instrument Waste Profile and all applicable MS
28. RI VREBRAT YFOAY APTOWUBERLET CORAL mfeagoUgROZ BIERECEASLICtmRUES CHIEFS gt REM CIRE U ETA CO RIS C EB ERE TD BI FACET OVEDHODDY Fun f ze MLE CORMROD do SARRIA SCALES ZI Ss riisniati sE SIC SU s CO Xm0O d Simi Abs OIBZLBZL S EIEEE ME US 2 C c Cem Sri BEENKDERNLETT Pb 6 Dg DR FALDT BLET FEEL TITITKESI RICART Y 2 Applied Biosystems RE CERINTULEALUADEAS LO YI TC cri c0 9203t 9KCHT3MREFRLET CORFO CHR TIRA BENE TWNSLOKGEADEWE SEEM ETH gt gt HENI N ra A I DAL 417 NE Sic Te A 71 339 Sot 0 218 A Ro ASIA on SE AERA LICH 0 A 40 AANI off AS LIEHBILILE 0 ASE EAFA 2 on off ES UEFA LICH 2 ee 0 USS Sete SAI ASO OSUCH et A2 AOL TA SAS BSA 2251010 A aes UEFA LICE 0 ASS WE Es ASS BAU PE USU 0 2157 UE m S AR ES ens BE QU veto ess LIEILIIIH ARASO E AS ILICE 9 Ol 2 e G ANES SVS Y Soh SIE AS SSL ere 3119 coll Ol 2582 118 ASS UEL 0 SH 2301 et SE VS Floll SAF SILIC
29. at output Deionized water is on site Proper waste disposal method for hazardous chemical waste has been established Fume hood or ventilation duct system is located within 5 m 15 ft of the instrument Equipment Waste Bottle with secondary containment Macintosh compatible printer this cannot be ordered with the instrument A two stage pressure regulator with dual gauges output range 0 80 psi high pressure range 0 3000 psi and a Compressed Gas Association CGA 580 cylinder adapter for each gas cylinder PROCISE PROCISE cLC Protein Sequencer Preinstallation Checklist continued v if Date ready Confirmed Components Approved strap or clamp to safely secure gas cylinders to the wall or bench table Consumable Supplies Additional Applied Biosystems reagent kits Additional computer supplies paper disks etc Chemically resistant powder free disposable gloves Two Argon gas cylinders of 99 998 purity or greater Acetone HPLC grade Methanol in wash bottle Sodium Dodecyl Sulfate SDS Site Preparation 1 7 Items Shipped with this Instrument Summary The PROCISE PROCISE cLC Protein Sequencer is shipped with the following Hazardous Chemicals Warning 1 8 Site Preparation User s manual Installation Chemical Kit P N 401690 and 402064 Apple Macintosh Power PC computer system Do not unpack any cartons related to
30. bter mais informacoes e que se proceda com cuidado gt P u HOO S mbolos A seguir apresentamos um gloss rio ilustrado de todos os s mbolos de alerta de n o el tricos seguran a n o relacionados eletricidade encontrados nos instrumento Applied Biosystems CUIDADO Este s mbolo representa um perigo devido a aquecedor no local Proceda com cuidado ao trabalhar em reas pr ximas a aquecedores para evitar queimaduras devidas ao contato com componentes quentes Este s mbolo indica que h um laser dentro do instrumento gt gt CHMBOJIbI OTHOCHIIHECH K 6e30IIacHOCTH 6 B npuBeneHHoN Huxe 1 npencraBIieH UNNIOCTPuUpoBaHHbl rnoccapul BCEX CUMBONOB CHMBOJIbI KOTOpble ncnonbsyioTca B npubopax Applied Biosystem instruments 07 OTHOLLIEHNE K anekTpuyeckon GesonacHoctTy KkaxaoM 0106 lt Takoro cumBona HEOGXOAUMO cobniogaTb COOTBETCTBYIOLUNe 00 0 o6ecneueHus 6esonacHocmM OTOT CUMBON 03HaWaeT YTO CETEBON 5 HAXOAUTCA BO BKJIIOHeHHOM NOSOKeHuu OTOT CUMBON O3HAUAET YTO CETEBON 5 HAXOAUTCA B BbIKJTOMEHHOM NONOXKEHUM OTOT CUMBON 06093
31. cendie remplacer uniquement par un fusible de m me type et de m me courant nominal CAUTION HOT Attention Surface chaude DANGER High voltage Danger Haut Voltage WARNING To reduce the chance of electrical shock do not remove covers that require tool access No user serviceable parts are inside Refer servicing to Applied Biosystems qualified service personnel Avertissement Pour r duire la chance du choc lectrique ne retirez pas les couvertures qui exigent l acc s d outil Aucune piece utile d ulitisateur n est int rieur R f rez l entretien au personnel de service de Applied Biosystems DANGER Laser radiation when open and interlock defeated Avoid direct exposure to beam Danger Rayonnement de Laser si ouvert et couplage a d fait Evitez l exposition directe au faisceau CAUTION Moving parts Attention Pi ces mobiles Safety Alert Symbols Electrical Symbols The following chart is an illustrated glossary of all electrical symbols that are used on Applied Biosystems instruments Whenever such symbols appear on instruments please observe appropriate safety procedures This symbol indicates the on position of the main power switch This symbol indicates the off position of the main power switch This symbol indicates the on off position of a push push main power switch This symbol indicates that a terminal may be connected to another instrument s
32. chneter Anschlu kann Wechselstrom oder spannung und Gleichstrom oder spannung erhalten oder abgeben VORSICHT Dieses Symbol zeigt das Vorliegen von Hochspannung an und warnt den Anwender mit Vorsicht fortzufahren VORSICHT Dieses Symbol fordert Sie auf das Handbuch zwecks n herer Informationen zu konsultieren und mit Vorsicht fortzufahren Nicht elektrische Die folgende Tabelle enth lt Beschreibungen aller auf den Instrumenten von Symbole Applied Biosystems verwendeten nicht elektrischen Symbole VORSICHT Dieses Symbol zeigt eine Gefahr durch die Heizung an Gehen Sie mit Vorsicht vor wenn Sie in der N he dieser Bereiche arbeiten damit Sie sich nicht an hei en Komponenten verbrennen Dieses Symbol zeigt das Vorliegen eines Lasers im Innern des Instruments an gt gt Simboli degli allarmi di sicurezza Simboli elettrici La tabella seguente un glossario illustrato di tutti i simboli elettrici utilizzati su strumenti Applied Biosystems Ogni volta che tali simboli compaiono sugli strumenti rispettare le procedure di sicurezza appropriate Questo simbolo indica la posizione ON dell interruttore di alimentazione generale Questo simbolo indica la posizione OFF dell interruttore di alimentazione generale Questo simbolo indica la posizione ON OFF di un interruttore di alimentazione generale a pulsante Questo simbolo indica che un terminale pu essere collegato al r
33. e and federal environmental health and safety regulations and laws Following are important points about the fume hood 4 The fume hood should operate continuously including nights and weekends because vented waste bottle contents can escape to surroundings The fume hood must be constructed of materials that are compatible with the waste materials chemicals being generated or exhausted 4 The fume hood should be located away from air currents generated by air conditioning ducts fans windows doors and moving equipment and persons The fume hood exhaust vent should be located where gaseous waste cannot be drawn back in the building Asign or label should be present that shows where to locate the fume hood sash so as to give an average flow of 100 ft min linear face velocity The minimum velocity flow at any point in the hood must be 80 ft min linear The maximum flow must not exceed 125 ft min linear The fume hood must meet local state and federal health and safety requirements Refer to current fume hood standards established by the American Society of Heating Refrigeration Air conditioning engineers ASHRAE American Conference of Governmental Industrial Hygienists ACGIH and Occupational Safety and Health Agency OSHA Check and record face velocity at least yearly Inspect and maintain exhaust system including fans and motors at least yearly It is important that the duct system ope
34. e Found On Your Instrument 2 2 leh S VINDOIS dais i 2 3 Electrical Symbols void 2 3 2 edi 4 2 3 Input Output ConnectIons 2 4 13 3111 1 59 2 4 SS AS EEEE TT 2 5 Pressurized Cras I a sm TT 2 5 Gascous Waste Sa oi io ire 31110909 9 2 5 77 seniors 2 6 Instrument Waste and Exhaust System 2 6 eee oe dar D TRE sis Tree 2 6 Conncetino the Bxhaust He 7109 2 6 Pressurized Gas and Accessories Needed but Not Supplied 2 7 Gas CV NAS int dinar LL ia ae ahs a ade cates asoka hoe es 2 7 Pressure Resulator raus bios odes Galas dass Rad edb aed hee AE nem 2 7 Zxttac hine the Cylinder A shua 2 7 Chemical Safety NSU ON 111 1 10 3 1 1 4 1 111 0 3 Hazardous Chemicals 2 AR 1 1 1 das 3 2 NN Nr 3 2 Handling Hazardous Chemicals 3 2 Hazardous Waste serra 3 33 TT DG 0 3 3 S cu I TT 3 3 Handling Hazardous Waste S ob ar 3
35. entos de Applied Biosystems Este s mbolo indica la posici n de encendido del interruptor principal Este s mbolo indica la posici n de apagado del interruptor principal Este s mbolo indica la posici n de encendido apagado de un interruptor principal de presi n Este s mbolo indica que existe la posibilidad de conectar esta terminal a la toma de tierra de referencia de otro instrumento Esta no es una toma de tierra protegida Este s mbolo indica que la toma de tierra protegida debe ser conectada a tierra antes de realizar cualquier otro tipo de conexion el ctrica al instrumento Una terminal marcada con este s mbolo recibe o suministra corriente o tensi n alterna Una terminal marcada con este s mbolo puede recibir o suministrar corriente o tensi n alterna y continua PRECAUCI N Este s mbolo indica la presencia de alta tensi n y advierte al usuario que proceda con precauci n gt PRECAUCI N Este s mbolo indica que consulte el manual para obtener m s informaci n y que proceda con precauci n S mbolos no A continuaci n se presenta un glosario ilustrado de todos los s mbolos de seguridad y el ctricos alerta no el ctricos que aparecen en los instrumentos de Applied Biosystems PRECAUCI N Este s mbolo indica peligro de altas temperaturas Proceda con cautela cuando trabaje cerca de estas zonas para evitar quemarse con componentes calient
36. es gt gt Este simbolo indica que hay un l ser dentro del instrumento Applied Biosystems ON
37. g the instrument adjacent to heaters or cooling ducts The installation category overvoltage category for this instrument is Il and it is classified as portable equipment The instrument has a pollution degree rating of 2 and may be installed in an environment that has non conductive pollutants only The thermal output of the instrument is 8800 Btu h 2 600 W Consult your facilities department regarding ventilation requirements for this level of heat output For our European customers any product marked with the CE label meets the European requirements for emission and immunity as defined in the EMC Directive 89 336 EEC This product has been evaluated to the Standard for Emissions for Industrial Scientific Equipment EN 55011 Class A and to the Standard for Generic Immunity EN50082 1 Laboratory Ventilation Venting Fume Hood Duct System This instrument produces toxic gaseous waste that must be ventilated either through a fume hood to a duct or directly to the duct The fume hood or duct must be located within 5 m 15 ft of the instrument The information presented here reflects U S regulations and practices for venting waste from Applied Biosystems instruments to a fume hood or to a duct WARNING Some Applied Biosystems instruments use chemicals that are hazardous Always mix and prepare hazardous materials under an operating fume hood CAUTION Dispose of all waste in accordance with all applicable local stat
38. ications personnel reaches into 150 countries on six continents For international office locations please call our local office or refer to our web site at www appliedbiosystems com www appliedbiosystems com ia Applied AS Bibsystems Applera Corporation is committed to providing the world s leading technology and information for life scientists Applera Corporation consists of the Applied Biosystems and Celera Genomics businesses Printed in the USA 01 2002 Part Number 904203E an Applera business
39. iferimento di terra del segnale di un altro strumento Non un terminale di terra protetto Questo simbolo indica un terminale protettivo di messa a terra che deve essere collegato a terra prima di realizzare qualsiasi altro collegamento elettrico allo strumento Un terminale contrassegnato con questo simbolo riceve o fornisce tensione o corrente alternata Un terminale contrassegnato con questo simbolo pu ricevere o fornire tensione o corrente alternata e continua ATTENZIONE questo simbolo indica la presenza di alta tensione e invita l utente a procedere con cautela ATTENZIONE questo simbolo invita l utente a consultare il manuale per ulteriori informazioni e procedere con cautela gt Simboli non elettrici Segue un glossario illustrato dei simboli degli allarmi di sicurezza elettrici trovati su strumenti Applied Biosystems ATTENZIONE questo simbolo illustra un rischio da alte temperature Procedere con cautela quando si lavora in queste aree per evitare ustioni causate di componenti a temperature elevate Questo simbolo indica la presenza di laser nello strumento gt gt RE LORY 7 BAIT 5 Y 7 rx PUQON 7 Applied Biosystems EEILERHANTOSLETOEANHI S v 2 TEIURUEST COLSRVY IMH REE BAT SBREUWT AICS EN FBRATYFOAYVOMBERLET FBRATYFOAIOMBERLET HU
40. ir davantage d informations et de proc der avec pr caution Symboles non Le tableau suivant donne la signification des symboles d alertes de s curit non lectriques lectriques qui figurent sur les appareils Applied Biosystems ATTENTION Danger associ la pr sence d un appareil de chauffage Proc der avec pr caution pour viter de se br ler au contact de pi ces ou d l ments chauds Indique que l appareil renferme un laser gt gt Sicherheitswarnsymbole Elektrische Symbole Die folgende Tabelle enth lt Beschreibungen aller auf den Instrumenten von Applied Biosystems verwendeten elektrischen Symbole Wenn diese Symbole auf den Instrumenten erscheinen beachten Sie bitte die entsprechenden Sicherheitsvorkehrungen Dieses Symbol zeigt die EIN Position des Hauptnetzschalters an Dieses Symbol zeigt die AUS Position des Hauptnetzschalters an Dieses Symbol zeigt die EIN AUS Position eines Druck Zug Hauptnetzschalters an Dieses Symbol zeigt an da ein Anschlu an die Betriebserde eines anderen Instruments angeschlossen werden kann Dies ist kein gesch tzter Erdanschlu Dieses Symbol zeigt einen gesch tzten Erdanschlu an der geerdet werden mu bevor andere elektrische Anschl sse zum Instrument hergestellt werden Ein mit diesem Symbol gekennzeichneter Anschlu kann Wechselstrom oder spannung erhalten oder abgeben Ein mit diesem Symbol gekennzei
41. is one of the primary goals of installation Persons who are to be trained to operate the instrument should set aside two uninterrupted days to work with the Applied Biosystems service representative If this is not possible the installation should be rescheduled Calibration of the instrument and verification of performance will be performed by an Applied Biosystems service representative during the installation Reagents shipped with this instrument will be consumed during the process of setup and verification Before installation be sure to order additional chemicals and other supplies necessary for the ongoing operation of the instrument Do not unpack instruments Inspect instrument cartons and report any damage to your Applied Biosystems service representative For information and instructions about unpacking the Installation Chemical Kit see Items Shipped with this Product later in this chapter Site Preparation 1 1 Technical Support Contacting Technical Support To Contact Technical Support by E Mail Hours for Telephone Technical Support To Contact Technical Support by Telephone or Fax 1 2 Site Preparation You can contact Applied Biosystems for technical support by telephone or fax by e mail or through the Internet You can order Applied Biosystems user documents MSDSSs certificates of analysis and other related documents 24 hours a day In addition you can download documents in PDF format from the Applied Bi
42. isted in the table below Title Topic Section Chemical Product and Company Identification 1 Composition Information on Ingredients 2 Hazards Identification 3 First Aid Measures 4 Fire Fighting Measures Accidental Release Measures 6 Handling and Storage 7 Exposure Controls Personal Protection 8 Physical and Chemical Properties 9 Stability and Reactivity 10 Toxicological Information 11 Ecological Information 12 Disposal Considerations 13 Transport Information 14 Hegulatory Information 16 Other Information 12 How to Get Extra Additional copies of MSDSs for chemicals manufactured by Applied Biosystemss are Copies of MSDSs available at no extra cost Call your local representative or access the Applied Biosystems World Wide Web site at www appliedbiosystems com techsupport 3 10 Chemical Safety MSDSs for the PROCISE PROCISE cLC Protein Sequencer MSDSs for Applied The MSDSs for chemicals manufactured by Applied Biosystems and used with the Biosystems PROCISE PROCISE cLC Protein Sequencer follow this section They are Chemicals Biobrene Plus P N 902420 Methanol P N 901834 Premix buffer concentrate P N 902173 R1 5 PITC in heptane P N 901824 R2 N Methylpiperidine water MeOH P N 902533 R3 TFA P N 901812 R4A 25 TFA in water P N 901816 R5 acetonitrile P N 901817 S1 n Heptane P N 901818 S2B ethylacetate P N 901813 S3 n Butyl Chloride P N 9018
43. l be followed at all times The following safety equipment should be available fire extinguisher Halon eye wash safety shower eye and hand protection adequate ventilation first aid equipment spill clean up equipment protection from any sources of radiation lasers radioisotopes contaminated equipment radioactive wastes etc that may be present in the area where our engineer will be working Site Preparation 1 11 Laboratory Space Required Dimensions The PROCISE PROCISE cLC Protein Sequencer and the Macintosh computer have the following dimensions Component Width Depth Height Total Weight Instrument 126 0 cm 103 5 cm 111 5 cm 49 in 41 in 44 in 125 kg 275 Ib Computer 35 5 cm 43 0 cm 45 5 cm 14 in 17 in 18 in Location of The PROCISE PROCISE cLC Protein Sequencer must be located within 5 m 15 ft of a Instrument fume hood or ventilation duct system The instrument requires a laboratory bench or sturdy table of correct dimensions to accommodate the system The instrument must be accessible to the installer on all four sides The Apple Macintosh computer and printer should be on the same table as the PROCISE PROCISE cLC Protein Sequencer If this is impractical the computer must be located within 2 m 6 ft of the instrument s left side panel IMPORTANT The operator should be able to reach the instrument while viewing the computer screen C
44. lammable The bottom aqueous phase contains residual trifluoroacetic acid and is corrosive HANDLE THIS MATERIAL WITH EXTREME CAUTION DO NOT DISPOSE OF THIS WASTE IN SINKS OR DRAINS THIS MATERIAL SHOULD BE DISPOSED OF AS A REGULATED HAZARDOUS WASTE Section 2 APPROXIMATE COMPOSITION PROCISE Material TLV PEL STEL CAS Ethyl Acetate EtAc 40 400 ppm 400 ppm 141 78 6 Butyl Chloride 31 N A N A N A Water 17 Acetonitrile 4 40 ppm 40 ppm 60 ppm 75 05 8 Heptane 3 400 ppm 400 ppm 500 ppm 142 84 5 Trifluoroacetic acid 2 N A N A N A TFA Phenylisothiocyanate 1 N A N A N A PITC N methylpiperidine 1 N A N A 626 67 5 Methanol 1 200 ppm N A 250 ppm 67 56 1 N A Not Available NFPA Combined Health Rating is 3 Health 4 Flammability 3 Reactivity PROCISE CLC Material TLV PEL STEL CAS Butyl Chloride 45 N A N A N A Ethyl Acetate EtAc 26 400 ppm 400 ppm 141 78 6 Water 16 Heptane 400 ppm 400 ppm 500 ppm 142 84 5 Acetonitrile 4 40 ppm 40 ppm 60 ppm 75 05 8 Trifluoroacetic acid 2 N A N A N A TFA Chemical Safety 3 5 PROCISE CLC Phenylisothiocyanate 1 N A N A N A PITC N methylpiperidine 1 N A N A 626 67 5 Methanol 1 200 ppm N A 250 ppm 67 56 1 N A Not Available NFPA Combined Health Rating is 3 Health 4 Flammability 3 Reactivity Section 3 PHYSICAL DATA BOILING POINT
45. learance A clearance of 30 cm 1 ft is needed at the rear of the instrument to provide adequate ventilation Additional clearance is required for servicing Do not block access to the rear of the instrument Sufficient space within approximately 1 5 m 5 ft of the instrument is needed to safely secure at least one size 1A Argon gas cylinder Note Additional information related to compressed gas safety issues is provided in Chapter 2 Instrument Safety Sufficient space to the right side of the instrument is needed for solvent and waste bottles continued on next page 1 12 Site Preparation Typical Laboratory A typical laboratory layout for the PROCISE PROCISE CLC Protein Sequencer and Layout accessory equipment is shown in Figure 1 1 Power Power Color monitor Solvent PROCISE and 490 PROCISE Protein Sequencer PTH Waste System Bottles Mouse and mouse pad SB ESSES eS eS S ISSSS N SS SS gt SIA Figure 1 1 Typical laboratory layout for the PROCISE PROCISE cLC Protein Sequencer Sequencing System Site Preparation 1 13 Electrical Requirements Power Grounding Power Cords Voltage Quality Voltage Spikes 1 14 Site Preparation The electrical receptacle should have a dedicated 2 0 kVA power line and ground or a 2 0 kVA power line with a line conditioner or uninterruptible power supply UPS The electrical receptacle must be located
46. lus Peptide Synthesizers 1 800 899 5858 then press 15 1 508 383 7855 PNA Custom and Synthesis 1 800 899 5858 then press 15 1 508 383 7855 FMAT 8100 HTS System and Cytofluor 4000 Fluorescence Plate Reader 1 800 899 5858 then press 16 1 508 383 7855 Chemiluminescence Tropix 1 800 542 2369 U S only or 1 781 271 0045 1 781 275 8581 Applied Biosystems MDS Sciex 1 800 952 4716 1 650 638 6223 Outside North America Region Telephone Dial Fax Dial Africa and the Middle East Africa English Speaking and West Asia Fairlands South Africa 27 11 478 0411 27 11 478 0349 South Africa Johannesburg 27 11 478 0411 27 11 478 0349 Middle Eastern Countries and North Africa Monza Italia 39 0 39 8389 481 39 0 39 8389 493 Site Preparation 1 3 1 4 Site Preparation Region Telephone Dial Fax Dial Eastern Asia China Oceania Australia Scoresby Victoria 61 3 9730 8600 61 3 9730 8799 China Beijing 86 10 64106608 86 10 64106617 Hong Kong 852 2756 6928 852 2756 6968 Korea Seoul 82 2 593 6470 6471 82 2 593 6472 Malaysia Petaling Jaya 60 3 758 8268 60 3 754 9043 Singapore 65 896 2168 65 896 2147 Taiwan Taipei Hsien 886 2 22358 2838 886 2 2358 2839 Thailand Bangkok 66 2 719 6405 66 2 319 9788 Europe Austria Wien
47. n publishes recommended regulations for local or state governmenis in the United States Hazardous rating system developed by this Association Acronyms and Abbreviations A 1 Table 1 1 Acronyms and abbreviations continued Term Explanation NIOSH National Institute of Occupational Safety and Health USA recommends exposure levels and respirators OC Open cup testing for flash point OSHA Occupational Safety and Health Administration USA sets chemical exposure levels PEL Permissible Exposure limit The federal OSHA limit usually expressed as TWA for an 8 hour work shift PPM Parts Per Million Prop 65 A California Law requiring warnings for chemicals that are known to the state to be carcinogenic or cause reproductive harm RCRA Resource Conservation and Recovery Act RTECS Registry of Toxic Effects of Chemical Substances SARA Superfund Amendments and Reauthorization Act a federal act administered by EPA SCBA Self Contained Breathing Apparatus STCC Standard Transportation Commodity Code STEL Short Term Exposure Level published by ACGIH TC o Lowest published toxic concentration TLV Threshold Limit Value The ACGIH recommended TWA usually for an 8 hour work shift TWA Time Weighted Average UEL Upper Explosive Limit u or U Unknown UN United Nations This designation identifies hazardous chemicals in the process of world wide transportation
48. nstruments 7 7 7 7 1 1 1 9 0 1 1 12 Clearanee vs shor us A deh 1 12 1 1 1 13 Electrical 3 1 a A 1 14 Power za NT 1 14 CITOLUTICIHID oe oma 2 2 2 3 3 3 3 3 3111 5009 1 14 es ab a Ii 1 14 Noltads OMAN I n 1 14 tas Gre nie Rime 2 3337 1 iw Q huu Shoe 1 14 3 3 3 7 1 1 15 Laboratory Environmental Requirements 1 16 Won p 33 1 1111 11 1 16 Tempera and Humidity 0 1 16 E 17 0 5 ie do ee is 1 16 Heat 1111111111 0 1 16 Emission Immunity Statement 0 1 16 1 31 9 1 17 N Hills cic a tec 11117 1129 9 90 1 17 Eunice HOO02 E EA 1 1 1111 0 1 17 BSC 2 2 3 3 TT 1 17 2 Instrument Safety Instrument Sal ely 333 33 3 11 9 2 1 SO SU de 1 1 11 6 2 1 Ustr 3 a 2 1 Instrument Abe a Das EI li EP UE C nd M d 2 Salely Labels 2 11 177 311 2 1 Sistial WOIdS 11 TT 0 2 1 Labels That May B
49. opy or hood The open end of the tubing should not face into oncoming air movement through the duct or canopy Connect the exhaust line from the instrument to the waste bottle so that it drops vertically This prevents liquid and waste from accumulating and blocking the flow Make sure that the line leads to the fume hood or duct in a straight and upward direction The line must not create low points by dipping in a downward direction because condensation may collect and prevent proper flow through the instrument Pressurized Gas and Accessories Needed but Not Supplied Gas Cylinder Pressure Regulator Attaching the Cylinder Each laboratory must supply the required gas cylinder and accessories for installation This instrument requires one size 1 A argon gas cylinder that holds approximately 7 2 m3 257 ft of gas when full Use only prepurified argon of 99 998 or greater purity An additional argon cylinder should be ordered in advance for the ongoing operation of the instrument CAUTION Damage to the instrument and its products can result from using impure argon gasses other than argon or an inadequate amount of argon A two stage pressure regulator with dual gauges output range 0 80 psi high pressure range 0 3000 psi and a Compressed Gas Association CGA 580 cylinder adapter with a needle type shutoff valve on the exit side is required The needle valves should have Swagelok type end fittings ready for connection
50. osystems Web site please see the section To Obtain Documents on Demand following the telephone information below Contact technical support by e mail for help in the following product areas Product Area E mail address Genetic Analysis DNA Sequencing galab appliedbiosystems com Sequence Detection Systems and PCR pcrlab appliedbiosystems com Protein Sequencing corelab appliedbiosystems com Peptide and DNA Synthesis Biochromatography PerSeptive DNA PNA tsupport appliedbiosystems com and Peptide Synthesis systems CytoFluor FMAT Voyager and Mariner Mass Spectrometers LC MS apisupport sciex com or Applied Biosystems MDS Sciex api3 support sciex com Chemiluminescence Tropix tropix appliedbiosystems com Inthe United States and Canada technical support is available at the following times Product Hours Chemiluminescence 8 30 a m to 5 30 p m Eastern Time Framingham support 8 00 a m to 6 00 p m Eastern Time All Other Products 5 30 a m to 5 00 p m Pacific Time In North America To contact Applied Biosystems Technical Support use the telephone or fax numbers given below To open a service call for other support needs or in case of an emergency dial 1 800 831 6844 and press 1 Product or Telephone Fax Product Area Dial Dial ABI Prism 3700 DNA Analyzer 1 800 831 6844 1 650 638 5981 then press 8 DNA Synthesis 1 800 831 6844
51. presentative will contact you to verify that everything is checked off before the installation date Checklist Check off and enter date to ensure that all preparations have been made 1 6 Site Preparation PROCISE PROCISE cLC Protein Sequencer Preinstallation Checklist v if ready Date Confirmed Components General Received instrument s and verified that cartons are intact Read this Site Preparation and Safety Manual including MSDSs Verified that instrument s serial number s and system configuration as shown on the packing list are the same as ordered Set aside two uninterrupted days for in lab training during installation Unpacked and stored contents of Installation Chemical Kit Electrical A dedicated 2 0 kVA power line and ground or a 2 0 kVA power line with a line conditioner or UPS is in place At least four standard power outlets are within 2 5 m 8 ft of the instrument location Instrument voltage if specified on the packing list matches the voltage available in the laboratory Laboratory Laboratory bench is of correct dimensions to accommodate the system and is situated so that the instrument is accessible to the installer on all four sides Laboratory safety requirements as specified in this manual have been met Laboratory environmental requirements as specified in this manual have been met Room ventilation accommodates instrument he
52. quate ventilation at all times Do not breathe vapor Do not get in eyes on skin or on clothing Accidental contact should be washed immediately Keep away from heat sparks and flame Spill collection materials eye wash and safety shower should be in area of use OTHER This waste solution has strong solvent properties and will attack many forms of rubber plastics coating and finishes Section 10 ADDITIONAL INFORMATION When not directly attached to the instrument this waste material should be stored in a secure well ventilated location suitable for flammable materials Store away from light heat or potential ignition sources Contact the appropriate state hazardous waste regulatory agency for proper disposal procedures and lists of registered service companies THIS WASTE MATERIAL IS HAZARDOUS AND SHOULD ONLY BE HANDLED BY PERSONS THOROUGHLY TRAINED IN HAZARDOUS MATERIALS HANDLING PROCEDURES Chemical Safety 3 9 Material Safety Data Sheets Overview Material Safety Data Sheets provide information about physical characteristics health hazards safety precautions first aid spill cleanup and disposal procedures Read the MSDSs before handling reagents or interacting with the instrument For a complete list of acronyms and abbreviations used in MSDSs see Appendix A Contents of MSDSs Each MSDS is divided into 16 sections although not all sections apply to every chemical The titles of the 16 sections are l
53. r death Handle all chemicals as potentially hazardous Chemicals are classified as hazardous when they are physically hazardous or if they may cause health hazards upon acute or chronic exposure Physically hazardous chemicals are materials that are flammable combustible compressed gasses explosives oxidizers organic peroxides pyrophoric reactive or unstable or water reactive Chemicals that may cause health hazards are materials that are carcinogens toxic or highly toxic reproductive toxins irritants corrosives sensitizers heptagons nephrotoxins agents which act on the hematopoietic systems and agents which damage the lungs skin eyes or mucous membranes Important requirements for handling hazardous chemicals include Chemicals Read all applicable MSDSs before handling hazardous chemicals When replacing reagents always install the new bottle on the instrument Do not top off old bottles Some chemicals reduce the integrity of glass bottles and repeated use beyond six weeks may result in the bottle fracturing when it is pressurized during operation Provide secondary containment for all reagent bottles Do not store chemicals in direct sunlight or heat on or off the instrument Hazardous Waste Overview A Handling Hazardous Waste Storing Hazardous Waste The PROCISE PROCISE cLC Protein Sequencer generates hazardous chlorinated organic liquid and gaseous waste WARNING CHEMICA
54. rate continuously including nights and weekends because vented waste bottle contents can escape to surroundings beconstructed of PVDF tubing or other materials compatible with the waste materials being generated not come in contact with strong oxidizers bases or other chemicals that are incompatible with gaseous waste allow vapor or gas movement of 1000 2000 ft min 5 5 11 m s WARNING Do not connect the waste vent to a ductless hood or to a system that purifies filters air and returns it to the room Site Preparation 1 17 Instrument Safety Instrument Safety Safe Operation User Attention Words Before operating the instrument read the information in this section concerning hazards and potential hazards Ensure that anyone involved with the operation of the instrument is instructed in both general safety practices for laboratories and specific safety practices for the instrument Four user attention words appear in the text of all documentation for Applied Biosystems products Each word implies a particular level of observation or action that is significant to user safety or proper instrument operation Note Calls attention to useful information IMPORTANT Indicates information that is necessary for proper instrument operation CAUTION Cautions the user that a potentially hazardous situation could occur causing injury to the user or damage to the instrument if this information is ignored
55. s Instrument Safety 2 5 Disposing of Gaseous Waste Instrument Waste and Exhaust System Exhaust Line Tubing Connecting the Exhaust line 2 6 Instrument Safety The waste and exhaust system of the instrument is composed of a 3 8 in o d 0 95 cm exhaust line attached to a 2 5 gal 9 5 L polyethylene bottle in a secondary container This exhaust line conducts the waste fumes and gasses that enter the waste manifold to a fume hood or duct system for disposal Note In the 432 Peptide Synthesizer the waste and exhaust system has a 1 4 in o d exhaust line attached to a 1 gal 4 L polyethylene bottle CAUTION Be sure to place the waste bottle in a secondary container to minimize the possibility of leaks The fume hood or duct system must be operating whenever the instrument power is on or when there is waste in the waste container Exhaust line tubing should be polypropylene tubing of the shortest possible length and the straightest possible run Tubing length should not exceed 15 ft 4 5 m The exhaust line tubing should not have low points that can trap residue or condensation The exhaust line tubing should be fastened securely Use fasteners of polypropylene or Teflon Do not use brass as it corrodes Be careful not to puncture tubing 4 The tubing should be located away from sources of potential damage such as contact heat or flame 4 The tubing end should be placed as far as possible into the duct can
56. s necessary for operating the instrument each laboratory must provide its own source of high quality deionized water In addition to the Installation Chemical Kit the following equipment and supplies are needed for operation of the PROCISE PROCISE cLC Protein Sequencer 4 Waste bottle with secondary containment Macintosh compatible printer this cannot be ordered with the instument A two stage pressure regulator with dual gauges output range 0 80 psi high pressure range 0 3000 psi and a Compressed Gas Association CGA 580 cylinder adapter for each gas cylinder Approved strap or clamp to safely secure gas cylinders to the wall bench table or cart Additional Applied Biosystems reagent kits Additional computer supplies paper disks etc Chemically resistant disposable gloves lab coats and safety glasses or goggles Two Argon gas cylinders of 99 998 purity or greater Acetone HPLC grade Methanol in wash bottle Sodium Dodecyl Sulfate SDS Laboratory Safety Onsite We request that a representative from your laboratory be in the vicinity and available Representative to the Applied Biosystems engineer at all times while he or she is on site Required Safety Your laboratory has specific safety practices and policies designed to protect Equipment laboratory personnel from potential hazards both obvious and hidden that are present We expect that all applicable safety related procedures wil
57. signal ground reference This is not a protected ground terminal This symbol indicates that this is a protective grounding terminal that must be connected to earth ground before any other electrical connections are made to the instrument A terminal marked with this symbol either receives or delivers alternating current or voltage A terminal marked with this symbol can receive or supply an alternating and a direct current or voltage CAUTION This symbol indicates the presence of high voltage and warns the user to proceed with caution CAUTION This symbol alerts you to consult the manual for further information and to proceed with caution Non electrical The following is an illustrated glossary of all non electrical safety alert symbols found Symbols on Applied Biosystems instruments CAUTION This symbol illustrates a heater hazard Proceed with caution when working around these areas to avoid being burned by hot components gt gt This symbol indicates that a laser is present inside the instrument Instrument Safety 2 3 VERAS DEUS ABAE AMAR as BEL Bil AE e ATE S 2 be Un Ba AO A RS BARRE EES Pi AFTER EE in ZIEHT TTS RHEIN Apu ASIE ESC EUX DARA AY fee 52 BN He EAC UG A oc
58. to 25 in 6 35 mm o d tubing CAUTION Do not allow the cylinder pressure to drop below 300 psi Chemical vapors and or liquids can backflow into the pressure regulator resulting in serious damage to the instrument The second stage output of the regulator should be set at approximately 15 psi 103 5 kPa Attach the pressurized gas cylinder firmly to a wall or gas cylinder cart by means of approved straps or clamps WARNING Pressurized gas cylinders are explosive Attach the pressurized gas cylinder firmly to a wall or bench by means of approved straps or clamps Always cap the gas cylinder when not in use Instrument Safety 2 7 Chemical Safety Introduction Please read This chapter contains detailed information for this instrument about hazardous chemicals used if any hazardous waste produced and how to handle Where appropriate detailed Waste Profiles and Material Safety Data Sheets MSDSs are provided to ensure correct and safe operation of the instrument Applied Biosystems assumes that all operations in your laboratory will be conducted in accordance with safety practices detailed in Waste Profiles and MSDSs and with any applicable laws and regulations Chemical Safety 3 1 Hazardous Chemicals Overview A Handling Hazardous 3 2 Chemical Safety This instrument uses chemicals that are hazardous chlorinated organic liquids WARNING Hazardous chemicals can cause injury illness o
59. towels or pads and removed to a well ventilated area away from ignition sources Larger amounts 1 liter or more may be collected with an inert absorbent kitty litter or similar material or commercially available spill pillows designed for solvent collection This waste material must not be allowed to enter confined spaces such as a sewer because of the possibility of an explosion This instrument waste solution should be disposed of as a regulated hazardous waste by a properly permitted hazardous waste management facility in accordance with federal state and local regulations Recommend disposal methods include high temperature incineration and solidification for secure chemical landfill disposal Section 8 SPECIAL PROTECTIVE EQUIPMENT RESPIRATORY PROTECTION VENTILATION An MSHA or NIOSH approved respirator for organic vapors is recommended A supplied air or SCBA respirator is recommended for high vapor concentration and emergency situations Handle within a well ventilated area Minimize Open exposure to air PROTECTIVE GLOVES Butyl rubber or similar type resistant to chlorinated solvents EYE PROTECTION Safety glasses with side shields monogoggles or face shield OTHER PROTECTIVE EQUIPMENT As necessary to prevent skin contact 3 8 Chemical Safety Section 9 SPECIAL PRECAUTIONS PRECAUTIONS TO BE TAKEN Handle as a flammable poisonous liquid Maintain ade
60. uler ou d eliminer WARNING Risk of electric shock Disconnect power cord from supply before replacing fuses or removing power supply module from instrument Avertissement Risque de choc lectrique D lectrique D brancher le cordon d alimentation avant de remplacer les fusibles ou de retirer le block d alimentation de l instrument WARNING For continued protection against risk of fire replace only with Listed and Certified fuse of the specified type and ratings Avertissement Pour assurer une protection continue contre les risques d incendie remplacer les fusibles uniquement par des fusibles num r s et certifi s du type de courant nominal specifi s WARNING HOT LAMP Avertissement LAMPE CHAUDE WARNING HOT Replace lamp with an Applied Biosystems lamp Composants chauds Remplacer la lampe par une lampe Applied Biosystems WARNING Disconnect supply cord before opening Grounding circuit continuity is vital for safe operation of equipment Never operate equipment with grounding conductor disconnected Avertissement D brancher la corde d approvisionnement avant l ouverture La continuit de circuit au sol est essentiel pour l exploitation s re du mat riel N actionnez jamais le mat riel avec le conducteur debranch WARNING For protection against fire hazard replace only same type and rating of fuse Avertissement Afin d assurer la protection contre les risques d in
61. ut connections are labeled on the instrument Computer Pump communication Detector input Figure 2 1 Input Output connections on the PROCISE PROCISE CLC Protein Sequencer 2 4 Instrument Safety Gas Safety Pressurized Gas Safety Gaseous Waste Safety WARNING Pressurized gas cylinders are potentially explosive if not handled properly and can cause great damage and severe injuries This instrument uses a pressurized gas cylinder for operation Some kinds of rapidly leaking gas can displace normal atmosphere and cause suffocation If knocked over a pressurized gas cylinder can explode Keep in mind the following when working with gas cylinders Screw the gas cap on tightly when not in use and when transporting Attach the cylinder firmly to a wall or gas cylinder cart by means of an approved strap or clamp WARNING Handle all liquid solid and gaseous waste as potentially hazardous Gaseous waste can cause injury illness or death Instruments that use gas cylinders for operation emit gaseous waste Follow carefully the instructions provided in this chapter in the section entitled Disposing of Gaseous Waste and Exhaust and read the MSDSs and Waste Profiles in Chapter 3 Chemical Safety Keep in mind the following when working with instruments that use gas cylinders Some gaseous waste is toxic Vent according to instructions in the guide and all appropriate laws and regulation
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