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1. Figure 13a Exposure Toolbar Controls Macintosh Exposure Time The Exposure Time slider enables you to control the electronic shutter of the camera by manually selecting an exposure time Dragging the slider to the left decreases the value dragging to the right increases it You may also adjust the exposure time by clicking on the increment and decrement arrows beside the slider Available exposure times range from a shortest time of 1 10000 of a second to a longest time of 60 seconds This control is enabled only under manual exposure mode and is disabled under automatic exposure AE mode These settings are reflected in both the preview as well as the captured image Sensitivity Pull Down List or Popup Menu The Sensitivity pull down list or popup menu allows you to manually control the camera s hardware gain controls Analogous to a standard film camera s ISO sensitivity ratings these settings 50 100 200 400 control the amplification of image data signals resulting in varying sensitivity These settings are reflected in both the preview as well as the captured image Instruction Manual 91 Viewfinder Lite 92 Exposure Toolbar cont Exposure Mode Pull Down List or Popup Menu The Exposure Mode pull down list or popup menu allows you to select between automatic and manual exposure modes Automatic Exposure Under Automatic Exposure AE mode the camera automatically calculates
2. 135 Measuring Color Values 137 Viewing Informational Windows 138 Choosing the Foreground Color eeceeeeeeeneeceneeeie esse nono rcnnnn 140 Choosing the Background Color na nnnnnnrccrannnnnnnncc 142 Viewing Images Windows Only 144 Using Online Hel Pst coco in ead edited een eee 145 lo APN eo Eege 147 Title Bar Windows Only 148 Control Menu Windows On 149 Men Ba nac 151 File Meno a A ie a 152 Edit Menta A id 158 View A O 160 Window Men ri a tas 164 Help Menu tc tit aiden daa 166 leet 168 TODO iio ii A eae Ba ee ee 172 7 TWAIN Viewfinder Lite Windows Only EXOT UE 175 Using TWAIN Viewfinder Lite Windows Only 176 8 Studio Lite Photoshop Plugin Macintosh Only Example A amanan anamanna annain Padaria E aiaa 181 Using the Studio Lite Photoshop Plugin Macintosh On 182 9 Specificato E 183 10 Troubleshooting ssssssssssnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn nnmnnn 185 1 Installing the Hardware PCI Interface Card Installation 1 Turn off the computer and all peripherals before beginning the hardware installation process 2 Remove the computer s cover and then proceed to install your PCI interface card Most computers have a combination of PCI and other expansion slot types i e AGP ISA Your computer manual should identify the location of the PCI slots 3 Carefully pull out the metal access cover behind an empty expansion slot
3. 4 Start the setup exe program in the NT folder Follow the on screen instructions to complete the installation 5 Restart the computer Windows 2000 PCI Device Driver Installation 1 Before you turn your computer on be sure the PCI interface card is properly installed and that your camera is connected 2 Turn your computer on As Windows loads your computer will display a Digital Signature Not Found dialog box Press the Yes button to continue with the installation im gi pT ia ierg on gee 24 en arte peg a et eam pe Swe es oie EH Bu pa emm ds rra hr midir Aen ha ma P 3 At the following screen press the Browse button AA aj i Tin Sa prgn w ee FT Le Trasi Ier Seppe aree da ti a repr EH a rih m Lage cs he A ui Er Instruction Manual 15 16 Installing the Software on Windows 4 Insert the CD ROM into your CD ROM drive The device driver files are located in the folder Win2k under driver folder for Windows 2000 Specify the correct location of the files by selecting your CD ROM drive and choosing the Win 2k folder under driver folder When the file name penguin sys is displayed press the Open button 5 Press the OK button Installing the Application The camera software should be installed after the successful installation of the camera hardware and device drivers To install the software applications for the
4. Set them properly Instruction Manual Trouble shooting 185 Trouble shooting 186 Troubleshooting cont Problem Cause Remedy The colors in the The wrong color Calibrate the white images are not temperature is set in balance by specifying correct the white balance the white region with a control operation rectangular frame of the Auto White Balance Error messages Camera cable is not Connect camera cable occur when connected properly properly Viewfinder Lite or other application started Device driver is not Install the device driver installed in the computer TWAIN is not installed Install the TWAIN in the Windows Only computer properly Photoshop Plug in is not Install the Photoshop installed Macintosh only Plug in in the computer properly Windows If no operations can be activated by pressing any of the buttons restart Windows or the computer Macintosh If no operations can be activated by pressing any of the buttons or using a menu the machine hangs press Opt Cmd esc to exit the program If that does not work restart the computer Troubleshooting
5. 1 Click once on the desired image to make it the active image window 2 Using the Marquee tool specify a region of interest representing the portion of the image that you would like to cut 3 Click the Cut button located in the Studio Lite Toolbar Method C 1 Click once on the desired image to make it the active image window 2 Using the Marquee tool specify a region of interest Instruction Manual Studio Lite Clipboard A reserved section of memory that is used as a temporary holding area when you copy and paste or cut and paste from one application to another After you copy or cut data from your application it is viewable in the clipboard If you cut data from an application and do not paste it into another and you do not save the clipboard s contents as a file the data will be lost upon exiting the operating system or when the next copy or cut operation is performed 119 Studio Lite 120 Editing and Retouching Images cont representing the portion of the image that you would like to cut 3 Windows Use the following keyboard shortcut Ctrl X Macintosh Use the following keyboard shortcut Cmd X Method D 1 Click once on the desired image to make it the active image window 2 Using the Marquee tool specify a region of interest representing the portion of the image that you would like to cut 3 Click once directly within the specified region of interest using the ri
6. Mac PICT Studio Lite allows for the selection of the following color depths when saving images in the PICT format Monochrome 1 bit 16 Colors 4 bits 256 Colors 8 bits and 16 million colors 24 bits FlashPix Studio Lite allows for the selection of the following color depths when saving images in the FlashPix format 256 Grays 8 bits and 16 million colors 24 bits To save a captured image file for the first time Method A 1 Click once on the desired image to make it the active image Instruction Manual Studio Lite Bitmap Bitmap BMP is the standard Windows image format on DOS and Windows compatible computers Also known as a bump file it is a Windows and OS 2 bitmapped graphics file format It is the Windows native bitmap format and every Windows application has access to the BMP software routines in Windows that support it BMP files provide formats for 2 16 256 or 16 million colors 1 bit 4 bit 8 bit and 24 bit color FlashPix The FlashPix FPX format developed by Kodak is designed to speed the transfer and display of large high resolution files in applications that support the FlashPix technology Although Studio Lite is not a FlashPix optimized application it can open and save FlashPix files The FlashPix format supports grayscale and RGB color modes and does not support alpha channels JPEG The Joint Photographic Experts Group JPG format is an ISO ITU sta
7. Viewfinder Command The Viewfinder Command will bring the Viewfinder preview window to the top of the stack of open windows If the Viewfinder window is not currently open it will be opened by this command Open Image Window List The Open Image Windows List displays all open image windows by their filename and current zoom factor Instruction Manual Studio Lite 165 Studio Lite 166 Help Menu Windows Help The Help menu Figure 17 provides access to the detailed online help reference as well as information regarding the currently release of the Studio Lite application Help Topics Command About Studio Lite Command Figure 17 Help Menu Help Topics Command The Help Topics command displays the Help Topics dialog box Figure 18 with the following category tabs Contents Index and Find These tabs assist in navigating through the extensive online help reference offered in the Studio Lite application Help Ieper Ene EH Contests ims feet Cb is Riad ee bt ex Ir a i ind en ac in Pe ie CC Ce e Figure 18 Help Topics Dialog Box About Studio Lite Command The About Studio Lite command displays version and copyright information pertaining to the current release of the Studio Lite application Application Suite Help Menu cont Studio Lite Macintosh Help The Help menu Figure 18a provides access to detailed online help reference St
8. To select the exposure time Method A Drag the Exposure time slider located in the Exposure toolbar Dragging the slider to the left decreases the exposure time dragging to the right increases it Method B Click on the increment or decrement arrows located beside the Exposure Time slider Clicking on the left arrow decreases the exposure time clicking on the right arrow increases it Exposure settings are reflected in both the preview all Viewfinder Lite modes as well as the captured image However in Fast mode the previewed effect of the exposure setting is limited to a maximum exposure time of 1 4 second While the effect of longer exposure settings will be reflected in the captured image the effect in the Fast mode preview image is simulated in the Viewfinder Lite software by intensifying the brightness level and may not accurately reflect the final capture quality Instruction Manual Viewfinder Lite 59 Viewfinder Lite 60 Adjusting Camera Sensitivity The Viewfinder Lite application allows you to control the sensitivity of the camera by adjusting its hardware gain control Analogous to a standard film camera s ISO sensitivity ratings these settings 50 100 200 400 control the amplification of image data signals resulting in varying sensitivity To adjust the sensitivity 1 Click on the Sensitivity pull down list or popup menu located in the Exposure toolbar 2 Highlight one of the four prede
9. To select the paper source from which to print click on the Source button and highlight the correct paper source Available paper sources are dependent on the particular printer and will vary from model to model 7 Windows only Select either Portrait or Landscape to specify the Orientation in which you would like the selected image printed Instruction Manual Studio Lite PostScript A page description language PDL from Adobe that is used extensively on all computer platforms lt is the standard for electronic input in most commercial typesetting and printing houses Aspect Ratio The ratio of height to width of an image The aspect ratio of images captured by cameras is approximately 3 4 equal to that of standard computer monitors and television sets 113 Studio Lite 114 Printing an Image cont 8 Click OK to apply the changes To Preview a Printed Image 1 Click on the File menu 2 Select the Print Preview command A representation of the printed image will be displayed on the screen 3 Windows only If the printed image spans more than a single page you may use the Next Page and Prev Page buttons to view different pages Click on the Two Page button to view two consecutive pages simultaneously in a split screen view To return to the single page preview click on the One Page button 4 Windows only To magnify the view click the Zoom In button To decrease magnification click t
10. Use the following keyboard equivalent Cmd J Viewfinder Lite Windows Studio Lite Macintosh Viewing the Preview cont Viewfinder Lite Method C Click the Preview On Off button located in the Capture toolbar Disabling the preview covers the preview image with the splash screen but does not discontinue power to the camera Instruction Manual 33 Viewfinder Lite 34 Capturing Images The primary function of the Viewfinder Lite application is to allow for the capture of high resolution still images with the camera system To capture a still image Method A 1 Click on the Viewfinder menu 2 Select the Capture command Method B Windows Use the following keyboard shortcut Alt C Macintosh Use the following keyboard equivalent Cmd K Method C Click on the Capture button located in the Capture toolbar Method D Press the Expose button on the external handswitch optional Current Viewfinder Lite settings are automatically applied to the captured image Upon capture the image is automatically sent to the Studio Lite image editing application for post capture processing On the Macintosh the Viewfinder and Studio Lite functionality is integrated into the single Studio Lite application so that in what follows in this manual the Macintosh user can ignore references to Viewfinder and Studio being separate On the Mac there is only one applicatin involved Thus when the user captures an image
11. bie Ed e Drem erch Hei Edit Menu Options Menu Help Menu Figure 4 Menu Bar Macintosh The Menu Bar is the strip across the top of the display File Menu View Menu Options Menu Help Menu me Hiesfirader plisss Wir tele Tus 10 0 Srami he Edit Menu Viewfinder Menu Window Menu Figure 4a Menu Bar Instruction Manual Studio Lite 151 Studio Lite 152 File Menu The File Menu Figure 5 5a provides access to standard file manipulation commands common to many Windows applications These include New Open Close Close All Save Save As Revert Duplicate Print Print Preview Print Setup or Page Setup and Exit or Quit Windows only The filenames of the four most recently opened images are also displayed CE E Le Yee ou eri ji New Command i Dech Droe CD Open Command Close Command ius bra Chea Save Command Save As Command 5m i Pina Cit Revert Command Print Command p Pai Payee Print Preview Command Print Setup Command P See Iran GENOA eb in ie AEB me ie gat Send Command Save As Kee rieo A coil omman dle g Save As Wallpaper able pai e Centered Command Fe Recently Opened Images Exit Command Figure 5 File Menu p rat gess Pepp EM Merr Si Ciertas am L Biri ie Jirar mh ae Fs Dapikale Pago Aeien Pra Presas Feit image EF mail i Figure 5a File Men
12. image is simulated in the Viewfinder Lite software by intensifying the brightness level The Viewfinder Lite preview image may appear of substantially lower quality than the final captured image at exposure time settings slower than 1 4 second Zoom The Zoom mode provides a view which is zoomed on the center 25 of the camera image area This portion of the image is displayed at the full CCD resolution 640x480 portion of 1392x1040 providing the highest level of detail of all of the Viewfinder Lite modes The effect of the Sharpness setting located under the Options menu is previewed only in the Zoom mode and may assist in the focusing process The frame rate of the preview image is slower than Fast mode same as Full mode and is limited by the exposure time setting Full The Full mode provides a view of the entire camera image area by scaling the CCD resolution image into the 640x480 pixel preview area providing increased resolution over Fast mode but decreased detail over Zoom mode The frame rate of the preview image is slower than Fast mode same as Zoom mode and is limited by the exposure time setting To select the Viewfinder Lite mode Method A 1 Click on the Viewfinder menu 2 Select the Viewfinder Mode menu item Viewfinder Lite Windows Studio Lite Macintosh Selecting the Viewfinder Mode Viewfinder cont Lite 3 Select the desired Viewfinder Lite mode from the pull down menu Method B Clic
13. or the File Format radio button group Macintosh choose an individual image format type to save the file in that particular format If you decide to save the image in JPEG format you may also select the compression factor using a slider bar On Macintosh the slider appears when you click the JPEG radio button The lower the compression factor minimum value of 1 the greater the compression resulting in smaller image files and more image quality degradation The higher the compression factor maximum value of 100 the less the image data is compressed resulting in larger image files and less image quality degradation By default the compression factor is set to 75 for JPEG images 5 Specify the color depth in which you would like the image saved In the Colors pull down list or Bit Depth popup menu choose the desired setting Di rider BD ee lant H f E mobat Peng 13 D gr ngt Pa o 481 Iess leo Micra Foie pte 5 4 la Ma BS MOL Appia Dares d AS San thie darompni ao Camiri sre 1 lA A File Farm CPI Mar dd Tut Fish FI Por et p 16 ia Colors Ota E GEN later ara eek fe om gesi i E OO TA i 31 wy a Leye F t mal Figure 1a SaveAs Dialog Box Macintosh Studio Lite Saving Images cont 6 Enter a filename and select the location to which you would like the file saved 7 Click the Save button Method B 1 Click once on the desired image to make it the activ
14. toolbar This will allow you to reposition the spot meter 2 Click with the mouse Windows left mouse button somewhere within the live image to cause the spot meter to be relocated and the exposure time automatically recalculated based on the new region within its borders Three pre defined spot sizes are available 30 1 and 0 1 Each size denotes the percentage of the area which the spot region occupies to the capturing image To re center the spot meter Click on the Center Spot button located in the Capture toolbar To specify the spot size Method A 1 Click on the Spot pull down list or popup menu located in the Exposure toolbar 2 Highlight one of the three predefined spot sizes Method B Press the Spot button on the external handswitch optional to cycle between the three predefined spot sizes Viewfinder Lite Windows Studio Lite Macintosh Using Manual Exposure The Viewfinder Lite application supports fully manual exposure control In this mode the Viewfinder Lite allows for user defined exposure times ranging from a shortest time of 1 10000 second to a longest exposure time of 60 seconds To select the manual exposure mode 1 Click the Exposure Mode pull down list or popup menu located in the Exposure toolbar 2 Select Manual This will activate Exposure Time slider and disable the Spot and Exposure Adjust pull down lists or popup menus as well as the AE Lock controls
15. using the Viewfinder window the still image is immediately seen in a new window right in the same application Viewfinder Lite Windows Studio Lite Macintosh Capturing Images cont Viewfinder Lite If the user wishes to preserve any still image he she should save it in Studio Lite Windows only If more than 20 images are displayed at once by Studio Lite Windows OS operations may to become unstable and you will not able to save any images Instruction Manual 35 Viewfinder Lite Frame Rate Frame rate or refresh rate refers to the speed at which the preview image within the Viewfinder Lite image window is updated measured in frames drawn per secona In standard US NTSC television broadcasts 30 frames are drawn on the TV screen per second The more frames drawn per second the more fluid the motion shown on the screen 36 Selecting the Viewfinder Mode The Viewfinder Lite offers three selectable preview modes Fast Zoom and Full presented within the live Viewfinder Lite image window at a size of 640x480 pixels Fast The default selection the Fast mode provides a view of the entire camera image area This mode is suitable for positioning the subject rapid focusing and determining optimum brightness levels In Fast mode the previewed effect of the camera exposure time setting is limited to 1 4 second While the effect of slower speeds will be reflected in the captured image the effect in the preview
16. Figures 2 and 3 The device name for your camera is TWAIN Viewfinder Lite TWAIN Viewfinder Lite Using TWAIN Viewfinder Lite cont EEE KI am H S SS RX PELA H Figure 3 Select TWAIN Viewfinder Lite 3 Acquire the selected TWAIN source Figure 4 Instruction Manual TWAIN Viewfinder Lite 177 TWAIN Using TWAIN Viewfinder Lite Viewfinder cont Lite Figure 4 Acquire TWAIN Source The TWAIN Viewfinder Lite application window will appear Figure 5 You can now use this application to capture images directly to your TWAIN compliant host application 178 TWAIN Viewfinder Lite Using TWAIN Viewfinder Lite TWAIN Viewfinder cont Si E Res a 5 mmm en e ene pm mp PUSE pa Lar kl L ER It Figure 5 TWAIN Viewfinder Lite Instruction Manual 179 Macintosh Only Photoshop 8 Studio Lite Photoshop Plugin Studio Lite Plugin jdwex4 00 _ Instruction Manual 182 Studio Lite Photoshop Plugin Using the Studio Lite Photoshop Plugin Macintosh Only Images captured from the Digital Microscopy Camera System can be imported by Adobe Photoshop and compatible applications in two different ways Method A Launch Studio Lite and capture an image as described above in this manual save the captured image Quit Studio Lite and launch Photoshop or simply launch Photoshop with Studio Lite still open Inside Photoshop open the captured imag
17. H x 1050 V 1 5Mpixels No of Effective Pixels 1392 H x 1040 V 1 45Mpixels 640 x 480 1392 x 1040 2776 x 2074 pixels ISO 50 100 200 400 equivalent 10 bits color 30 1 or 0 1 selectable spot size Exposure Mode AE Manual AE Lock Exposure Adjust 2EV to 2EV 1 10 000 sec to 60 sec Accumulation Mode Average Integral Number of Accumulations 64 frames maximum White Balance Auto White Balance One Push White Balance Manual White Balance TIFF 8 bits 24 bits 48 bits BMP 8 bits 24 bits JPEG 8 bits 24 bits PICT 8 bits 24 bits FlashPix 8 bits 24 bits Windows only Mode EXPOSE AE LOCK SPOT PCI Bus Interface Preview Frame Rate 10 frames sec maximum Operating Environment Indoor Use Altitude Max 2000 meters Ambient Temperature 5 C 35 C 41 F 95 F Maximum relative humidity 80 for temperature up to 31 C 88 F decreasing linearly through 66 at 35 C 95 F Power supply voltage fluctuation Not to exceed 10 of normal voltage Pollution degree 2 in accordance with IEC 664 Installation over voltage category II in accordance with IEC 664 Instruction Manual Specifications 183 10 Troubleshooting Under certain conditions performance of the system may be adversely affected by factors other than defects If a problem occurs please review the following list and take remedial act
18. Studio Lite application workspace The following commands are available New Window Cascade Tile Arrange Icons and Close All The Open Windows list displays all opened image windows by their filenames and current zoom factor New Window Command Tile Command Cascade Command Arrange Icons Command Close All Command id T Open Windows List Figure 16 Window Menu New Window Command The New Window command creates a new image window containing a duplicate of the image in the active image window All manipulations made to this image will be reflected in the original and vice versa Cascade Command The Cascade command arranges all open image windows diagonally in the workspace from left to right and top to bottom Tile Command The Tile command arranges all open image windows as non overlapping tiles left to right top to bottom Arrange Icons Command The Arrange Icons command arranges icons for all minimized images in the workspace neatly along the bottom of the workspace Application Suite Window Menu cont Close All Command The Close All command closes all image windows prompting you to save any altered or unsaved images Open Windows List The Open Windows List displays all open image windows by their filenames and current zoom factor Macintosh ei Camera Miri mir image NATT tr ULT EEN K Figure 16a Window Menu Macintosh
19. The Open button allows you to open any supported image file into a new image window Save Button The Save button saves the image in the active image window to Studio Lite Toolbar cont Studio Lite disk Cut Button The Cut button removes the selected area from the active image window and places it in the clipboard Studio Lite fills the empty space with the current background color If no region of interest is selected the entire image is cut Copy Button The Copy button copies the selected area to the clipboard without removing it from the active image window If no region of interest is selected the entire image is copied Paste Button The Paste button places the image content in the clipboard into the active image window Undo Button The Undo button reverses the most recent operation performed on the image in the active image window Redo Button Windows only The Redo button re applies the previously undone action Viewfinder Lite Button Windows The Viewfinder Lite button starts up the Viewfinder Lite application and powers up the camera displaying a live image of the current camera subject in the Viewfinder Lite image window Instruction Manual 169 Studio Lite Application Suite Toolbar cont Macintosh The Viewfinder Lite button will bring the Viewfinder preview window to the top of the stack of open windows If the Viewfinder window is not currently open it wil
20. active image window 2 Click the View menu 3 Select the Fit to Screen command This option scales the view and the active image window size to match the Studio Lite application window size To change the view to fill the screen Method A 1 Click once on the desired image to make it the active image window 2 Click the View menu 3 Select the Full Screen command Method B 1 Click once on the desired image to make it the active image window Instruction Manual Studio Lite 133 Studio Lite Magnifying and Reducing the View cont 2 Click the Full Screen button located in the Studio Lite Toolbar This option scales the view to display the active image in the entire screen Press Esc to return to the previous image size 134 Application Suite Working Within the Studio Lite Application Window Windows Only Studio Lite allows for the customization of the viewing properties of the Studio Lite application window To enable the full screen view of the Studio Lite application Method A 1 Click the View menu 2 Select the Edit View command 3 Choose the Full Screen option Method B Use the following keyboard shortcut F7 This option will enable a full screen view of the Studio Lite application window with a menu bar but no Title Bar or Status Bar To restore the default view of the Studio Lite application Method A 1 Click the View menu 2 Select the Edit View command 3 Choose
21. as the side and bottom scrollbars To pan an image click and hold the mouse button Windows left button on any region of the image and drag the pointer in the direction that you would like the image moved Instruction Manual 173 Studio Lite 174 Status Bar Windows Only The Status Bar Figure 21 provides current information for the active image including Tool Status Mouse Pointer Coordinates Color Channel Values Foreground Color and Background Color Tool Status Color Channel Values Foreground Color Lat Be ridad pad Dl cl nn a RIR HHD bhii if Mouse Pointer Coordinates Background Color Figure 21 Status Bar Tool Status The Tool Status displays information regarding the currently selected tool as well as descriptions of the Toolbar and menu options Mouse Pointer Coordinates The Mouse Pointer Coordinates display the horizontal X axis and vertical Y axis pixel coordinates for the current mouse pointer position Color Channel Values The Color Channel Values display the Red R Green G and Blue C color channel values for the current mouse pointer pixel position Foreground Color Double clicking on the Foreground Color patch will cause the Color dialog box to open allowing you to select a custom color The Foreground Color is used with the Fill Color option Background Color The Background Color displays the current color selected for the background pertaining to the Eraser tool as wel
22. camera 1 Insert the application CD ROM disc into your CD ROM drive 2 Double click the setup exe file located on the root level of the CD ROM This will start the installer program Figure 1 biooenns Dira Cra Gem el A Be El ii red ad all ame pra Pr e Se migdia A Fenr Figure 1 Launch Installer Program 3 You will be prompted to close all currently running programs before continuing with the installation process Figure 2 Verify that no other programs are running and click the Next button to continue Installing the Software on Windows Installing the Software on Windows 2 Installing the Software on Windows cont Dardo jee ha he ic om Dll lr peng i pepe sal ral ira Llega Ciara oeicgeteig A As Page baks rra dl Dee pee Lk Cannel bo ua Dar arepa ci Ierseng Dict Pima ae aire mies EM an jmpe vo med jee rg ri bra wel cr Pee eth edocs oo cleo col A Cp ge ca a a il rs el Eed a gef zl e pc doped by Be ma pm rd meee ar e bes E y J Ciia Figure 2 Close All Active Program and Click the Next Button 4 The Install Selection dialog box will appear Figure 3 Select the components which you would like installed from the following choices Both installations are selectable at the same time Viewfinder Lite and Studio Lite This option will install both the Viewfinder Lite and Studio Lite software applications TWAIN Module This option will install the TWAIN driver allowing for direct image
23. capture to most TWAIN compliant applications Chd da conca pal od ha ll H gees Lt sai edo s Figure 3 Install Selection Instruction Manual 17 2 Installing the Software on Installing the Windows cont Windows 5 The Choose Destination Location dialog box will be presented Figure 4 Accept the default destination path or click the Browse button to manually select a custom path When you are finished click the Next button Chas Cord e gen E e v i iibi a Ce i ig E Taro a beia iot Fiat Fiz laa M e es Pego anal reari ee f Fontan cher ned tq rri Pieces log Lames be klug ee ee a gesch Ena Wm Esa Figure 4 Choose Destination Location 6 The Select Program Folder dialog box will appear Figure 5 Accept the default program folder name or specify a different folder by entering a custom folder name or by selecting an existing folder Click the Next button to begin the program installation process Veter Pa E ada A zsm es denk fei vpe E ha be tege ps bes g ree bikr rar rg m r pra Safe ir Forza Cii Has io caria Figure 5 Select Program Folder 18 Installing the Software on Windows Installing the Software on Windows Each camera is shipped with a floppy diskette containing custom characteristics data generated by that particualar camera unit This data allows for the calibration of capture settings for maximum performance 2 Installing the Software on Wi
24. channel 7 Click the OK button to apply the level adjustment settings and close the property sheet or dialog box Macintosh Level adjustments are automatically applied in real time to both the preview image and to the corresponding histogram To freeze the histogram so that it does not change due to noise to changes in the scene viewed by the camera or to adjustments of the markers check the Freeze checkbox Method B Instruction Manual 53 Viewfinder Lite 54 Adjusting Levels cont 1 Click on the Settings button located in the Options toolbar This will bring up the Settings property sheet or dialog 2 Repeat steps 3 through 8 above To reset level adjustment settings Method A 1 Click on the Options menu 2 Select the Settings command This will bring up the Settings property sheet or dialog box 3 Click the Level Adjustment tab 4 Click the Reset button 5 Level Adjustment level will be reset to the default settings 6 Click OK to close the property sheet or dialog Method B 1 Click on the Settings button located in the Options toolbar This will bring up the Settings property sheet or dialog box 2 Repeat steps 3 through 6 above To save user preset settings Method A 1 Click on the Options menu 2 Select the Settings command This will bring up the Settings property sheet or dialog Viewfinder Lite Windows Studio Lite Macintosh Adjusting Le
25. eave invert the THER drive labeled Camera Gata isin the THETH Drive H ou ds not have a EHA press Cancel io et values Cua Figure A 7 Camera Data Select Confirmation Window Install Default Characteristics Data from the CD ROM This option will install a non specific standard set of characteristics data Select this option if you do not have access to the original Camera Data CD ROM supplied with your camera Click the OK button following the instruction in the display window Installing the Software on Macintosh 3 Installing the Software on Installing the Macintosh cont Software on Macintosh 8 If the Studio Lite with Photoshop Plug in was selected in section 5 you will be presented Figure A 8 Proceed to the next step pushing the install button The installation will not be completed if Quit button was selected Repeat from section 1 to complete the installation E TIT E AAA rice Pag io Figure A 8 Start Window for the Plugin Installation 9 You will be presented Figure A 9 Select the folder where the Photoshop Plug in will be set up into Then click the Install into Plug ins button Plug ins portion may be different depending on the selected folder name Pieca og Photoshop plug ing folders la MiobesPhotoshops 55 8 Macnieshear IT Desktop me Cancel TRUI MB Treo pp Chis disk ital ite PHug ins Figure A 9 Folder Select Window for the Pl
26. factor ranging Application Suite View Menu cont from 12 to 50 Zoom Factor Command The Zoom Factor command brings up the Zoom Factor dialog box which allows you to manually select a zoom factor ranging from 6 to 1600 with a slider bar Zoom Factor Dialog Box The Zoom Factor dialog box Figure 10 allows you to manually select a zoom factor ranging from 6 to 1600 with a slider bar Figure 10 Zoom Factor Dialog Box Actual Pixels Command The Actual Pixels Home command displays the active image at its actual physical size 100 Fit in Window Command The Fit in Window command optimizes the active image s zoom ratio to fit within the active image window Fit to Screen Command The Fit to Screen command optimizes the active image s size and zoom ratio to fill the Studio Lite application window Full Screen Command The Full Screen command hides the Studio Lite and Windows interface filling the screen with the current image This enables you to view an image without the distractions of other open Instruction Manual Studio Lite 161 Studio Lite 162 View Menu cont windows or programs To return to Windows press Esc Edit View Command Windows Only The Edit View command presents a sub menu which allows you to choose either a full screen view with menu bar F7 or restore the view to the default Toolbar Option Windows Only The Toolbar F3 o
27. make it the active image window 2 Click the Save button located in the Studio Lite Toolbar After completing the save operation the selected filename of the saved image should be displayed in the title bar of the active image window Studio Lite Correcting Mistakes Studio Lite allows for the correction of mistakes made while editing an image Most single operations can be undone if a mistake is made You can also restore an altered image to its last saved state To reverse the last performed operation Method A 1 Click on the Edit menu 2 Select the Undo command Method B Use the following keyboard shortcut Ctrl Z Method C Click the Undo button located in the Studio Lite Toolbar If an operation cannot be reversed the Undo command will not be available as an active selection To re apply the previously un done operation Windows only Method A 1 Click on the Edit menu 2 Select the Redo command Method B Use the following keyboard shortcut Ctrl Y Method C Instruction Manual Studio Lite 111 Studio Lite 112 Correcting Mistakes cont Click the Redo button located in the Studio Lite Toolbar If a previously un done operation cannot be re applied the Redo command will not be available as an active selection To reverse all changes made to an image since it was last saved Method A 1 Click on the File menu 2 Select the Revert command Method B Use th
28. marker triangle the middle marker to specify the desired gamma level for the selected color channel 7 Windows Click the OK button to apply the level adjustment settings Macintosh Level adjustments are automatically applied in real time to both the preview image and to the corresponding histogram To freeze the histogram so that it does not change due to noise to changes in the scene viewed by the camera or to adjustments of the markers check the Freeze checkbox Method B 1 Click on the Settings button located in the Options toolbar Viewfinder Lite Windows Studio Lite Macintosh Adjusting Levels cont Viewfinder Lite This will bring up the Settings property sheet or dialog box 2 Repeat steps 3 through 7 above To adjust the highlight value Method A 1 Click on the Options menu 2 Select the Settings command This will bring up the Settings property sheet or dialog 3 Click the Level Adjustment tab 4 Click on the Channel pull down list or popup menu 5 Select the color channel upon which you would like the adjustment performed You may choose from the composite RGB channel or the individual color channels Red Green and Blue 6 Windows Adjust the Highlight slider bar to specify the desired highlight level for the selected color channel Macintosh Adjust the Highlight marker triangle the right hand marker to specify the desired highlight level for the selected color
29. mode is suitable for positioning the subject rapid focusing and determining optimum brightness levels In Fast mode the previewed effect of the camera exposure time setting is limited to approximately 1 4 second While the effect of longer exposure times will be reflected in the captured image the effect in the preview image is simulated in the Viewfinder Lite software by intensifying the brightness level The Viewfinder Lite preview image may appear of substantially lower quality than the final captured image at exposure time settings slower than 1 4 second Zoom Mode The Zoom mode Viewfinder Lite provides a view which is zoomed on the center 25 of the camera image area This portion of the image is displayed at the full CCD resolution 640x480 portion of 1392x1040 providing the highest level of detail of all of the Viewfinder Lite modes The effect of the Sharpness setting located under the Options menu is previewed only in the Zoom mode and may assist in the focusing process The frame rate of the preview image is slower than Fast mode same as Full mode and is limited by the exposure time setting Full Mode The Full mode Viewfinder Lite provides a view of the entire camera image area by scaling the CCD resolution image into the 640x480 pixel preview area providing increased resolution over Fast mode but decreased detail over Zoom mode The frame rate of the preview image is slower than Fast mode same as Zoom mode and is l
30. on the Capture Size command 3 Select the desired capture size from the pull down menu Method B Windows Click on the Capture Size pull down list located in the Capture toolbar Highlight one of the three predefined image capture resolutions Macintosh Locate the Capture Size popup menu located in the controls below the image pane and select the desired image capture resolution Each capture resolution setting offers varying levels of photographic detail and image file size In general higher resolution images offer increased detail in a larger file size Uncompressed images captured at resolutions of 640x480 1392x1040 and 2776x2074 pixels have file sizes of approximately 900KB 4 15MB and 16 2MB respectively The resultant effect of the capture resolution selection is not reflected by the live image displayed in the Viewfinder Lite image window Viewfinder Lite Windows Studio Lite Macintosh Quitting the Viewfinder Lite Application To close the Viewfinder Lite application For Windows users Method A 1 Click on the Viewfinder menu 2 Select the Exit command Method B Click once on the x button located at the far right of the Viewfinder Lite application window s title bar Method C Double click the Control Menu icon located at the far left of the Viewfinder Lite application window s title bar Method D 1 Click once on Control Menu icon located at the far left of the Viewfinder Lite app
31. spot meter is centered in the Viewfinder Lite image window The effect of the spot meter position on exposure time is dynamically updated in the preview image Center Spot Button The Center Spot button relocates the spot meter to the center of the Viewfinder preview window AE Lock Button The AE Lock button switches between the activation and deactivation of the auto exposure lock feature of the Viewfinder Lite application When AE Lock is selected the exposure time and gain are locked at their current settings temporarily disabling the auto exposure function When this button is not selected the automatic exposure feature is re enabled Viewfinder Lite Windows Studio Lite Macintosh Exposure Toolbar Viewfinder Lite The Exposure Toolbar provides the functions necessary for camera exposure control These include Exposure Time Sensitivity Exposure Mode Spot and Exposure Adjust Windows only The Exposure toolbar can be manually repositioned to any location on the Windows desktop using mouse drag and drop Double clicking on the upper border of the Exposure Toolbar will return it to the Viewfinder Lite application window Exposure Time Exposure Mode Pull Down List Exposure Adjust Pull Down List Eas IN P HEN fi lr om Leefer Egaam ode DI pmm O Usch Sensitivity Pull Down List Spot Pull Down List Exposure Warning Indicator Figure 13 Exposure Toolbar A 3 Pes 2
32. the standard Windows Color dialog box 2 Repeat steps 3 and 4 above To select a custom Background Color Method A 1 Click on the Options menu 2 Select the Background command This will bring up the standard Windows Color dialog box Application Suite Choosing the Background Color Studio Lite cont 3 Press the Define Custom Colors button 4 Specify a custom color by entering either Hue Saturation and Luminance values or values for the Red Green and Blue color channels 5 Press the Add to Custom Color button 6 The specified color will be displayed under Custom Colors Click once on the desired color 7 Click OK to apply the change Method B 1 Double click on the Background Color displayed in the lower right corner of the Studio Lite application window This will bring up the standard Windows Color dialog box 1 Repeat steps 3 to 7 above The current Background Color selection displayed in the lower right corner of the Studio Lite application window will be updated to reflect the change Macintosh Studio Lite uses the Background Color to fill in erased areas of an image The current Background Color is displayed at the right hand end of the Main Toolbar along with the current Foreground Color To select the Background Color double click on the Background Color patch displayed on the Main Toolbar the right hand patch This will bring up the standard Macintosh Color Picker dialog b
33. the optimum exposure time based on the region of the image contained within the spot meter The Exposure Time controls are disabled under this mode Manual Exposure Under Manual Exposure mode exposure times may be specified manually using the Exposure Time controls The Spot and Exposure Adjust controls are disabled under this mode Spot Pull Down List or Popup Menu The Spot pull down list or popup menu allows you to select the size of the spot meter The sizes are specified as an area percentage of the capture area Available sizes are 30 1 and 0 1 Under automatic exposure mode the exposure time is calculated based on the image data present within the region defined by the spot meter This control is enabled only under automatic exposure AE mode and is disabled under the AE Lock and manual exposure modes Exposure Adjust Pull Down List or Popup Menu The Exposure Adjust pull down list or popup menu allows you to fine tune the exposure of images under automatic exposure mode The Exposure Adjust range is 2 EV to 2 EV by 1 3 EV steps Exposure Warning Indicators Two indicators Over and Under feedback regarding whether an image is over exposed or under exposed This control is enabled only under automatic exposure AE mode and is disabled under manual exposure mode Viewfinder Lite Windows Studio Lite Macintosh Options Toolbar Viewfinder Lite The Options toolbar Figure 14 provides function
34. the same time Using the Installer 1 Insert the Setup CD ROM disk into your CD ROM drive 2 Double click the CD ROM Install CD icon that appears on your desktop to view ts contents Figure A 1 Double Click the CD ROM Icon Instruction Manual 21 Installing the 3 Installing the Software on Software on Macintosh cont Macintosh 3 Inside the CD ROM s Finder window double click the Studio Lite Installer icon This will launch the Studio Lite installation program Figure A 2 Double Click the Studio Lite Installer Icon 4 The Studio Lite informational screen will be displayed Click Continue to proceed with the installation process Studio Lite Installer Macmtasn Figure A 3 Studio Lite Informational Screen 22 Installing the Software on Macintosh 3 Installing the Software on Installing the Macintosh cont pal ei acintos 5 In order to use the Digital Microscopy Camera System from within Adobe Photoshop or compatible application you must select Studio Lite with Photoshop Plugin instead of the default Studio Lite when the Install Selection window is showing This will instruct the installer to copy the file Viewfinder into the Photoshop folder If you are not planning to use the camera with Photoshop select Studio Lite S ee lone the tlm Alo NA AA PA na Pia gia Figure A 4a Install Selection Window 6 A dialog box will appear p
35. 4 Remove the PCI interface card from its anti static bag Hold the card by its edges to avoid touching its circuitry 5 Align the connector end of the PCI card over the empty PCI slot Push the card down gently but firmly until it fits in the slot 6 Secure the PCI card and replace the cover to your computer 7 Connect the camera to the interface card using the included PCI interface cable If you have the external handswitch optional insert the plug into the proper connector on the card Instruction Manual Installing the Hardware Warning Never connect or disconnect your camera if your computer power is turned on To avoid damaging your computer or the PCI interface card do not attempt to install the PCI card without first checking the documentation for your computer Before touching the internal parts of your computer or installing any components discharge potential static electricity by placing your hand on any unpainted metal surface in the computer Installing the Hardware 10 1 Installing the Hardware cont 8 Turn the computer on Be sure that the camera is properly connected to the PCI interface card before you turn the power back on Mounting the Camera A proper C mount adapter is necessary to be able to attach your camera to your microscope In general to optimize image quality and field of view an adapter designed for use with a 1 2 CCD with an appropriate magnificati
36. Adjust the Shadow slider bar to specify the desired shadow level for the selected color channel Macintosh Adjust the Shadow marker triangle the left hand marker to specify the desired shadow level for the selected color channel 7 Windows Click the OK button to apply the level adjustment settings Macintosh Level adjustments are automatically applied in real time to both the preview image and to the corresponding histogram To freeze the histogram so that it does not change due to noise to changes in the scene viewed by the camera or to adjustments of the markers check the Freeze checkbox Method B 1 Click on the Settings button located in the Options toolbar Instruction Manual 51 Viewfinder Lite 52 Adjusting Levels cont This will bring up the Settings property sheet or dialog 2 Repeat steps 3 through 7 above To adjust the gamma value Method A 1 Click on the Options menu 2 Select the Settings command This will bring up the Settings property sheet or dialog box 3 Click the Level Adjustment tab 4 Click on the Channel pull down list or popup menu 5 Select the color channel upon which you would like the adjustment performed You may choose from the composite RGB channel or the individual color channels Red Green and Blue 6 Windows Adjust the Gamma slider bar to specify the desired gamma level for the selected color channel Macintosh Adjust the Gamma
37. As long as the Studio Lite application is still running you can always open the Viewfinder window again or bring it to the top of any other open windows by selecting Viewfinder in the Window menu The box on the right side of the Title Bar is called the Collapse Box i i SC Soa Me e a a Tat Te CR I ora Figure 2a Viewfinder Window Title Bar Macintosh When Studio Lite is the current application its menus appear in the Menu Bar of your computer Figure 4a me Hiesfirader Epligns Mirada Hei Tis 10 0 Srn He Figure 4a Menu Bar Macintosh Instruction Manual 69 Viewfinder Lite 70 Viewfinder Menu The Viewfinder Menu Figure 5 duplicates the features found in the Capture Toolbar including Start Preview Capture Capture Size White Balance On Off Black Balance and AE Lock In addition this menu allows access to certain Focus and Options toolbar features such as Viewfinder Mode Focus On Off and Focus Reset ET e fede Geom Uer Start Preview Command Iwer Pos hae a yt Capture Command has dl 1 Viewfinder Mode METEO Fas Capture Size Command Cate See b Eom Fa Focus On Off Command Tore Focus Reset Command dn dd White Balance Black Balance On Off Puestas Command Command AE oui AE Lock Command Exit Command _ s Figure 5 Viewfinder Menu Epitana UA Ma Dags SI Cagtaug aK Start Preview Comm
38. BUSY Busy LED AE Lock Button SPOT Busy Expose Button Spot Button External Handswitch Expose Button The Expose button duplicates the Capture function of the Viewfinder Lite application by instructing the camera to capture a still image of the current camera subject to the Studio Lite application applying the defined Viewfinder Lite capture settings i e capture resolution exposure etc and image processing options i e white black balance color balance etc Instruction Manual Installing the Hardware 11 Installing the Hardware 12 1 Installing the Hardware cont AE Lock Button The AE Lock button duplicates the AE Lock function of the Viewfinder Lite application by toggling between the activation and deactivation of the auto exposure lock feature of the Viewfinder Lite application When the AE Lock feature is enabled the AE Lock button in the Capture toolbar is automatically activated fixing the exposure time and gain at their current settings temporarily disabling the auto exposure function When AE Lock is off the automatic exposure feature is re enabled Spot Button The Spot button allows you to select the size of the spot meter The sizes are specified as an area percentage of the capture area As the button is depressed the spot size cycles through each of the available spot sizes in the following order 30 1 and 0 1 Under automatic exposure mode the exposure
39. E Lock Button Capture Size Pull Down List Move Spot Button Option Pull Down List Black Balance Button Figure 12 Capture Toolbar Ki H CIR Iesse Sie A Figure 12a Capture Toolbar Controls Macintosh Preview On Off Button The Preview On Off button controls power to the camera When this button is depressed the camera is powered on displaying a constantly refreshed live image preview of the current camera subject in the Viewfinder Lite image window Otherwise the camera is powered off and the Viewfinder Lite splash screen is displayed in the image window Capture Button Windows The Capture button instructs the camera to capture a still image of the current camera subject to the Studio Lite application applying the defined Viewfinder Lite capture settings i e capture resolution exposure etc and image processing options i e white black balance color balance etc Instruction Manual 87 Viewfinder Lite 88 Capture Toolbar cont Capture Button Macintosh The Capture button instructs the camera to capture a still image of the current camera subject and place it in a new window applying the defined capture settings i e capture resolution exposure etc and image processing options i e white black balance color balance etc Capture Size Pull Down List or Popup Menu The Capture Size pull down list or popup menu allows for the selection of one of three pr
40. Instruction Manual Windows 95 98 2000 NT MacOS Instruction Manual Windows 95 98 2000 NT MacOS Software and manual Copyright 1995 2001 Pixera Corporation All rights reserved Pixera Corporation reserves all rights to trademarks patents and copyrights involved in Pixera Corporation software documentation images and other products and collateral No part of this document may be reproduced or transmitted in any form or by any means electronic or mechanical for any purpose without the express permission of Pixera Corporation Information in this document is subject to change without notice This device complies with part 15 of the FCC Rules Operation is subject to the following two conditions 1 This device may not cause harmful interference and 2 this device must accept any interference received including interference that may cause undesired operation Warning The connection of a non shielded equipment interface cable to this equipment will invalidate the FCC certification of this device and may cause interference levels that exceed FCC limits It is the responsibility of the user to obtain and use proper equipment and cables Changes and modifications not expressly authorized by Pixera Corporation may void warranties All other company and product names are trademarks or registered trademarks of their respective owners The camera unit PCI interface board and handswitch bear the CE marking because they comply with th
41. Studio Lite Windows To close a displayed image window choose from one of the following five methods Method A 1 Click once directly on the image you wish to close to make it the active image window 2 Click on the File menu 3 Select the Close command Method B 1 Click once directly on the image you wish to close to make it the active image window 2 Click once on the x button located at the far right of the image window s title bar Method C 1 Click once directly on the image you wish to close to make it the active image window 2 Double click the Control Menu icon located at the far left of the image window s title bar Method D 1 Click once directly on the image you wish to close to make it the active image window 2 Click once on Control Menu icon located at the far left of the image window s title bar Instruction Manual 105 Studio Lite 106 Closing an Image Window cont 3 Select the Close command Method E 1 Click once directly on the image you wish to close to make it the active image window 2 Use the following keyboard shortcut Ctrl F4 To close all opened image windows 1 Click on the Window menu 2 Select the Close All command You may be prompted to save any images which have not been saved or that have been altered since the last save operation took place Macintosh Use the close box or file menu Close to close a single image window The keyb
42. The Settings property sheet allows you to customize the image processing functions of Viewfinder Lite with the following tabs Color Balance Level Adjustment and User Preset Color Balance Tab The Color Balance tab Figure 7 allows you to select from one of four white balancing schemes Off One Push White Balance Auto White Balance and Manual Color Correction Black balancing may also be applied here Instruction Manual Viewfinder Lite 75 Viewfinder Lite 76 Options Menu cont Cora bere mni chair es erent Show Balance tatir biore Rectangle Of CD a Checkbox E amig bairr Auto White White Balance e RETRATA Palance ie geet Manual Color AAA A Ak Correction PA al Ei A ej pr Bech Enteros Black Balance P z a ie Ke er E ww Checkbox E x iaa e E ka Show Balance Rectangle Checkbox Le j tm Figure 7 Setting Dialog Box Color Balance Tab EL RG Calar Eater Leet Mijas tree yf Weer Peeve i H ef e fa iia LiU iria eat h deed jin i Figure 7a Settings Dialog Box Color Balance Panel Macintosh Off The Off setting disables all white balancing functions No white balance operations will be performed in either the preview or captured image Viewfinder Lite Windows Studio Lite Macintosh Options Menu cont Viewfinder Lite Auto White Balance The Auto White Balance setting allows the user to calibrate the white balancing by s
43. and Capture Command Viewfinder Mode meinir raar F Cai tire te Capture Size Command Focus On Off Command Term mewn Focus Reset Command White Balance Command Mibibs Haispce E Black Balance On Off AE Lock Command Command Figure 5a Viewfinder Menu Macintosh Start Preview Command The Start Preview command Windows Alt S Macintosh Cmd J controls power to the camera When selected the camera is powered on displaying a constantly refreshed live image preview of the current camera subject in the Viewfinder Lite image window When this command is not selected the camera is powered off and the Viewfinder Lite splash screen is displayed in the image window Capture Command The Capture command Windows Alt C Macintosh Cmd k instructs the camera to capture a still image of the current camera subject to the Studio Lite application applying the defined Viewfinder Lite Windows Studio Lite Macintosh Viewfinder Menu cont Viewfinder Lite Viewfinder Lite capture settings i e capture resolution exposure etc and image processing options i e white black balance color balance etc Viewfinder Mode Command The Viewfinder Mode command allows for the selection of one of three different preview display modes Fast Zoom and Full Fast Mode The default selection the Fast mode Viewfinder Lite provides a view of the entire camera image area This
44. c 3 ts Instruction Manual 13 14 Installing the Software on Windows 2 Installing the Software on Windows cont 5 Press the Have Disk button 6 Press the Browse button 7 Insert the CD ROM into your CD ROM drive The device driver files are located in the folder Win98 for Windows98 or in the folder Win95 for Windows95 Specify the correct location of the files by selecting your CD ROM drive and choosing the Win98 or Win95 folder When the filename pixera inf is displayed press OK 8 Ensure that the proper directory for the driver is displayed in the field Copy manufaturer s files from If the proper directory is not displayed press the Browse button and specify the correct directory for the driver Press OK 9 Press Next 10 Windows will ask you if you are sure you want to use this driver Press Yes 11 Follow the on screen prompts to complete the installation Windows NT PCI Device Driver Installation 1 Before you turn your computer on be sure the PCI interface card is properly installed and that your camera is connected 2 Turn your computer on and log on to Windows NT You must have full administrative privileges in order to successfully install the drivers Installing the Software on Windows 2 Installing the Software on Installing the Windows cont Software on Windows 3 Insert the CD ROM into your CD ROM drive
45. capture mode 1 Select the bright field mode or the fluorescent mode 2 in Fig 1 2 Select Auto or Manual setting 3 in Fig 1 3 Select the capturing image size 4 in Fig 1 Set the spot meter size Select the spot meter size 5 in Fig 1 Adjust framing and focusing of specimen Move spot meter and EV adjustment Move the spot meter on the specimen 6 in Fig 1 Adjust the exposure value 7 in Fig 1 Adjust white balance and black balance Adjust WB If necessary adjust BB 8 in Fig 1 Capturing Capture images by pushing the capture button 9 in Fig 1 Check Image Check captured image quality Instruction Manual 27 28 Capturing 4 Capturing Procedure cont Procedure Figure 1 Viewfinder Lite Capturing Procedure 5 Viewfinder Lite Windows Viewfinder Studio Lite Macintosh Lite d yl Viewfinder Lite Version 1 0 SIISE Instruction Manual 29 Viewfinder Lite Taskbar The Taskbar is the bar on the Windows desktop that includes the Start button Buttons representing programs currently running on your computer appear on this bar 30 Running the Viewfinder Lite Application Windows The Viewfinder Lite application is the first step in acquiring images with the digital camera system To run the Viewfinder Lite application 1 Click on the Start button located on the Windows taskbar 2 Point to Programs The Programs menu wil
46. ce on the desired image to make it the active image window 2 Click on the View Menu 3 Select the Zoom Factor command This will bring up the Zoom Instruction Manual 131 Studio Lite 132 Magnifying and Reducing the View cont Factor dialog box 4 Using the mouse drag the slider to the desired preset zoom factor 5 Click OK to apply the change To display an image at 100 Method A 1 Click once on the desired image to make it the active image window 2 Click the View menu 3 Select the Actual Pixels command Method B 1 Click once on the desired image to make it the active image window 2 Windows Use the following keyboard shortcut Home Method C 1 Click once on the desired image to make it the active image window 2 Click on the Zoom button located in the Studio Lite Toolbox When placed over the active image the mouse pointer will be designated by a magnifying glass 3 Hold down the Ctrl key and click once anywhere within the active image window Application Suite Magnifying and Reducing the View cont To change the view to fit the active image window 1 Click once on the desired image to make it the active image window 2 Click the View menu 3 Select the Fit in Window command This option scales the view to match the active image window size To change the view to fit the Studio Lite application window 1 Click once on the desired image to make it the
47. creased detail over Zoom mode The frame rate of the preview image is slower than Fast mode same as Zoom mode and is limited by the exposure time setting Instruction Manual 95 Viewfinder Lite 96 Focus Toolbar The Focus toolbar Figure 15 gives you access to the focusing tools in Viewfinder Lite These include Focus On Off Reset Focus Current Focus Level Indicator and Optimum Focus Level Indicator This tool represented by the Current Focus Level Indicator and Optimum Focus Level assists in the focusing process for stationary subjects Click once on the Focus toolbar with your mouse to switch between a graphical or numerical user interface Windows only The Focus toolbar can be manually repositioned to any location on the Windows desktop using mouse drag and drop Double clicking on the upper border of the Focus toolbar will return it to the Viewfinder Lite application window Optimum Focus Level Focus On Off uttan Te ebe Focus Reset Button Current Focus Level Figure 15 Focus Toolbar r Figure 15a Focus Toolbar Controls Macintosh Focus On Off Button The Focus On Off button toggles between the activation and deactivation of the Focus Indicator as well as the Focusing Target in the Viewfinder Lite image window Focus Reset Button The Focus Reset button sets the Optimum Focus Level to the Current Focus Level and is useful when you would like to calibrate the Focus Indicator on a n
48. cts of the Studio Lite application window Restore Move Size Minimize Maximize and Close Restore Command Move Command Size Command 5 sien Minimize Command Maximize Command Hass aCe Close Command Figure 3 Control Menu Restore Command The Restore command returns the Studio Lite application window to its previous state Move Command The Move command allows for the manual positioning of the Studio Lite application window within the Windows desktop using the keyboard s directional arrow keys Size Command The Size command allows for the manual sizing of the Studio Lite application window using the keyboard s directional arrow keys Minimize Command The Minimize command reduces the Studio Lite Pro application window to an icon in the Windows taskbar Maximize Command The Maximize command increases the size of the Studio Lite application window to fill the entire Windows desktop Close Command Instruction Manual 149 Studio Lite Control Menu Windows Only cont The Close command closes the Studio Lite application window and quits the program 150 Application Suite Menu Bar Windows The strip below the Title Bar is called the Menu Bar Figure 4 Control over the range of image editing tools offered by Studio Lite is available through the six menu selections File Edit View Options Window and Help File Menu View Menu Window Menu
49. d The About Viewfinder Lite command displays version and copyright information pertaining to the current release of the Instruction Manual Viewfinder Lite 85 Viewfinder Lite 86 Help Menu cont Viewfinder Lite application Macintosh Help The Help menu Figure 10a provides access to detailed online help reference Studio Lite Help Command The Studio Lite Help command displays online help in a new window faut Saigon hai thom Dalarnas die biie Paip Figure 10a Help Menu Macintosh The window displays the same information as this printed manual and can be searched for specific topics or keywords Viewfinder Lite Windows Studio Lite Macintosh Capture Toolbar Viewfinder Lite The Capture Toolbar Figure 12 provides the functions necessary for basic camera control These include Preview On Off Capture Capture Size Accumulation Option Auto White Balance Black Balance One Push White Balance Photometry Photometry Centering and AE Lock Windows only The Capture toolbar can be manually repositioned to any location on the Windows desktop using mouse drag and drop Double clicking on the upper border of the Capture toolbar will return it to the Viewfinder Lite application window Auto White Balance Button Accumulation Setting One Push White Balance Button Capture Button Center Spot Button aj Fa e alejas Preview On Off A
50. d under Custom Colors Click once on the desired color 7 Click OK to apply the change Method B 1 Double click on the Foreground Color displayed in the lower right corner of the Studio Lite application window This will bring up the standard Windows Color dialog box 2 Repeat steps 3 to 7 above Macintosh Studio Lite uses the Foreground Color for the fill color function The current Foreground Color is displayed at the right hand end of the Main Toolbar along with the current Background Color To select the Foreground Color double click on the Foreground Color patch displayed on the Main Toolbar the left hand patch This will bring up the standard Macintosh Color Picker dialog box Instruction Manual 141 Studio Lite 142 Choosing the Background Color Windows Studio Lite uses the Background Color to fill in erased areas of an image The current Background Color is displayed in the lower right corner of the Studio Lite application window along with the current Pen 8 Brush Color To select the Background Color Method A 1 Click on the Options menu 2 Select the Background command This will bring up the standard Windows Color dialog box 3 A list of 48 available colors are displayed Click once on the desired color 4 Click OK to apply the change Method B 1 Double click on the Background Color displayed in the lower right corner of the Studio Lite application window This will bring up
51. ds the raw image data of the specified number of accumulated frames Frame integration strengthens weak image signals by accumulating image data but also has the effect of increasing any noise which is present in the accumulated images and is only suitable for still subjects The effect in the captured image due to the frame integration operation is not reflected in the live image displayed in the Viewfinder Lite image window Auto White Balance Button Pressing the Auto White Balance button allows the user to select with the cursor a rectangular area of the image which the application will use to set the white balance automatically You may thus set any arbitrary region of the live image to be the standard by which white is defined This region remains in effect until a different mode of color balancing is selected the Focus Finder is activated or the Move Spot button is pressed to on Once this region is specified the white balancing is automatically updated to provide optimum color reproduction This feature should be selected when viewing images which have discernible regions of true white These settings are reflected in both the preview as well as the captured image Black Balance Button The Black Balance button acts in a similar fashion to the White Balance button Pressing it permits the user to draw a rectangular area that will be used as the standard for black Once this region is specified the black balancing is automa
52. e Grayscale Button The Toggle Grayscale button converts the preview image in the Viewfinder Lite image window from color to shades of gray This change is reflected in the captured image as well Settings Button The Settings button brings up the Settings dialog box which allows you to customize the image processing functions of Viewfinder Lite with the following tabs Color Balance Level Adjustment and User Preset Brightfield Button The Brightfield button BF works in conjunction with the automatic exposure mode AE to optimize exposure settings for image capture under brightfield microscopy Fluorescence Button The Fluorescence button FL works in conjunction with the automatic exposure mode AE to optimize exposure settings for image capture under fluorescence microscopy The fluorescence mode can have a different target exposure than brightfield mode when the Viewfinder is set to automatic exposure mode These parameters are defined in the file settings dat If the fluorescence parameter is lower than brightfield it will converge on a darker setting causing the preview image to appear darker in FL mode Fast Button The Fast button displays a view of the entire camera image area within the Viewfinder Lite image window Taking the image directly from the CCD the fastest possible frame rate is achieved by sub sampling every other pixel horizontally and every fourth pixel vertically This mode is sui
53. e file Proceed to manipulate the file as you would any other file that Photoshop can manipulate Method B Launch the Photoshop application In Photoshop s File menu select the Import sub menu and the Viewfinder menu item found there This menu item will only appear if you have previously installed the Studio Lite Plugin by selecting Studio Lite with Photoshop Plugin when you ran the Studio Lite installer The result of selecting Viewfinder will be the launching of the Studio Lite application While in this mode Studio Lite when the user presses the Capture button will transfer the captured image to Photoshop You can continue to capture as many still images as you want with all of them going to Photoshop When you wish to return to Photoshop use the Mac OS application menu or simply click on one of the Photoshop windows When Photoshop is once again the running application it will terminate Studio Lite You can also exit Studio Lite by using Quit in its file menu Proceed to manipulate the file in Photoshop as you would any other file that Photoshop can manipulate After switching back to Photoshop you can select Viewfinder to perform additional image captures at any time Studio Lite Photoshop Plugin 9 Specifications Specification Single Chip CCD Color Camera with Light Swing Cooling System Electronic Cooling with Peltier Device 1 2 1 500 000pixel single chip color CCD No of Total Pixels 1434
54. e following keyboard shortcut Alt R Studio Lite Printing an Image Studio Lite provides basic functions for outputting captured images to a variety of printing devices The general steps for printing an image are setting up the printer previewing the image and performing the print operation To set the printer options 1 Click on the File menu 2 Windows Select the Print Setup command This will bring up the standard Windows Print Setup dialog box Macintosh Select the Page Setup menu item in the File menu This will bring up the Print Setup dialog box You can then select the printer and its paper size the image s orientation on the paper and the scaling percentage 3 Windows only If more than one printer is configured on your system you have the option of selecting which printer you would like to print from To select the printer click on the Printer pull down menu and highlight the desired printer 4 Windows only Click the Properties button to customize printer settings This will bring up the dialog box for the selected printer Available options are dependent on the particular printer and will vary from model to model 5 Windows only Click on the Paper Size pull down list to select a paper size which corresponds to the particular print media that you will be using Available paper and media types are dependent on the particular printer and will vary from model to model 6 Windows only
55. e image window 2 Click on the File menu 3 Select the Save As command The Save As dialog box should appear 4 Repeat steps 4 to 7 above Method C 1 Click once on the desired image to make it the active image window 2 Click the Save button located in the Studio Lite Toolbar The Save As dialog box should appear 3 Repeat steps 4 to 7 above To save an existing image in a different file format 1 Click once on the desired image to make it the active image window 2 Click on the File menu 3 Select the Save As command The Save As dialog box should appear 4 Repeat steps 4 to 7 above Instruction Manual Studio Lite Os and 1s The more patterns can be found the more the data can be compressed Graphics files can generally be compressed to about 20 to 90 of its original size Compression ratios depend entirely on the type of file and compression algorithm used The LZW Lempel Ziv Welch compression method stems from two techniques introduced by Jacob Ziv and Abraham Lempel LZ77 creates pointers back to repeating data and LZ78 creates a dictionary of repeating phrases with pointers to those phrases 109 Studio Lite 110 Saving Images cont To save an image in its current format Method A 1 Click once on the desired image to make it the active image window 2 Click on the File menu 3 Select the Save command Method B 1 Click once on the desired image to
56. e requirements of the directive 89 336 EEC concerning electromagnetic compatibility 1 2 Getting Ready 4 The camera system uses precision components Handle it with care and avoid subjecting itto a sudden or severe impact The image displayed on the display monitor may be affected when used near equipment generating strong electromagnetic waves This is not a malfunction and will not affect the actual image being recorded To avoid interference during operation keep the unit far from any source of electromagnetic waves Do not use the camera in areas where it may be subject to direct sunlight high temperature and humidity dust or vibrations For operating environment conditions see section 8 Specifications on page 182 When not using the camera system keep it covered with the dust cover provided with the microscope Do not connect or disconnect the camera when the power of computer is on Maintenance and Storage 1 Be careful not to leave dirt or fingerprints on the lenses and other glass components If a lens is dirty wipe it gently with a piece of gauze To remove fingerprints or oil smudges wipe the lens with gauze slightly moistened with a mixture of ether 70 and alcohol 30 or pure alcohol Since solvents such as ether and alcohol are highly flammable they must be handled carefully Be sure to keep these chemicals away from open flames or potential sources of electrical sparks
57. e the property sheet or dialog Method B 1 Click on the Settings button located in the Options toolbar This will bring up the Settings property sheet or dialog box 2 Repeat steps 3 through 6 above Method C 1 Point the camera at a white target for microscopy applications remove a slide place a blank slide on the microscope or move the slide so that a blank or white region of the specimen is positioned into the field of view 2 Click on the Viewfinder menu Instruction Manual 45 Viewfinder Lite 46 Adjusting the Color Balance cont 3 Select the White Balance command and select One Push Method D 1 Point the camera at a white target for microscopy applications remove a slide place a blank slide on the microscope or move the slide so that a blank or white region of the specimen is positioned into the field of view 2 Press the One Push White Balance button located in the Capture toolbar The One Push White Balance will be calibrated according to the target white reference used To enable and calibrate Auto White Balance Method A 1 Click on the Options menu 2 Select the Settings command This will bring up the Settings property sheet or dialog 3 Click the Color Balance tab 4 Select Auto White Balance 5 Click OK to close the property sheet or dialog box 6 To specify a reference white area within the Viewfinder Lite image window Click and hold the left mou
58. edefined image capture resolutions 640x480 1392x1040 and 2776x2074 Accumulation Setting The Accumulation setting allows you to specify the number of frames to be captured by the Viewfinder Lite application for frame averaging or integration operations You may select a value between 1 single capture no accumulation and 64 by clicking the up down arrows or by typing a number in the box Option Pull Down List or Popup Menu The Option pull down list or popup menu allows you to select either of the following two frame accumulation options Average or Integral Average The Average operation sums adds the raw image data of the specified number of accumulated frames and divides the resulting signal by the number of frames summed Frame averaging results in an improvement of the signal to noise ratio S N of the captured image by a factor equal to the square root of the number of frames averaged up to sqrt 256 16 This operation reduces the random noise generated under low light conditions i e darkfield fluorescence microscopy while maintaining contrast brightness and color and is only suitable for still subjects The enhancement in the captured image due to the frame averaging operation is not Viewfinder Lite Windows Studio Lite Macintosh Capture Toolbar cont Viewfinder Lite reflected in the live image displayed in the Viewfinder Lite image window Integral The Integral operation sums ad
59. egion of interest representing the portion of the image that you would like to copy 3 Click once directly within the specified region of interest using the right mouse button 4 Select the Copy command The copied image data is stored in the Windows Clipboard and will be available for use by Studio Lite or other Windows applications until the Clipboard data is deleted or replaced by other data Instruction Manual 121 Studio Lite 122 Editing and Retouching Images cont To paste the contents of the Windows Clipboard into the active image Method A 1 Click once on the desired image to make it the active image window 2 By default the image data is pasted into the upper left corner of the active image window To denote a specific position for the pasted data specify a region of interest representing the destination area using the Marquee tool 3 Click the Edit menu 4 Select the Paste command Method B 1 Click once on the desired image to make it the active image window 2 By default the image data is pasted into the upper left corner of the active image window To denote a specific position for the pasted data specify a region of interest representing the destination area using the Marquee tool 3 Click the Edit menu 4 Click the Paste button located in the Studio Lite Toolbar Method C 1 Click once on the desired image to make it the active image window 2 By default the
60. elected the 16 Bits Channel option allows captured images to be processed with 16 bits of data per color channel red green and blue The increased dynamic range offered by the resulting 48 bit RGB images is particularly effective in increasing image detail during accumulation operations including frame averaging and integration Sharpness Option The Sharpness option allows you to set the use of the sharpening filter in the Viewfinder Lite application Four varying settings for Instruction Manual 83 Viewfinder Options Menu cont Lite sharpening are available Off Minimum Medium and Maximum 84 Viewfinder Lite Windows Studio Lite Macintosh Help Menu Windows Help The Help menu Figure 10 provides access to the detailed online help reference as well as information regarding the current release of the Viewfinder Lite application i tele Help Topics Command Hs Tepes iboi VeraFinde Lin About Viewfinder Lite Command Figure 10 Help Menu Help Topics Command The Help Topics command displays the Help Topics dialog box Figure 11 with the following category tabs Contents Index and Find These tabs assist in navigating through the extensive online help reference offered in the Viewfinder Lite application Contests Leck fei Ciao lacada ial De oler Elia Elo lc mis ado ah a eben M Hie KEE Figure 11 Help Topics Dialog Box About Viewfinder Lite Comman
61. ence microscopy applications To optimize the automatic exposure mode for brightfield microscopy Click on the Brightfield BF button located in the Options toolbar To optimize the automatic exposure mode for fluorescence microscopy Click on the Fluorescence FL button located in the Options toolbar The fluorescence mode can have a different target exposure than brightfield mode when the Viewfinder is set to automatic exposure mode Exposure settings are reflected in both the preview all Viewfinder Lite modes as well as the captured image However in Fast mode the previewed image with a long exposure time may different from the captured image to keep the fast frame rate While the effect of longer exposure settings will be reflected in the captured image the effect in the Fast mode preview image is simulated in the Viewfinder Lite software by intensifying the brightness level and may not accurately reflect the final capture quality Instruction Manual 57 Viewfinder Lite 58 Using the Spot Meter The spot meter works in conjunction with the automatic exposure feature of the Viewfinder Lite application to accurately compute the appropriate exposure time for a given scene The spot meter may be repositioned within the Viewfinder Lite image window or adjusted in size to customize the region upon which the exposure is calculated To reposition the spot meter 1 Click on the Move Spot button located in the Capture
62. ently Opened Images lists the paths and filenames of up to four of the most recently opened image files Each file can be opened by clicking on its filename Exit Command Windows Only The Exit command closes the Studio Lite application window and quits the program Quit Command Macintosh Only The Quit command quits the Studio Lite application and closes all its windows Instruction Manual Studio Lite 157 Studio Lite 158 Edit Menu The Edit menu Figure 8 provides access to standard file editing commands common to many applications These include Undo Redo Cut Copy and Paste Commands specific to Studio Lite are Paste as New Image Crop Clear Del Fill Select All and Deselect Le Wa Ugen icine P Undo Commana ls D Redo Command Cut Command fe EI Tom orb Copy Command Paste Command Zen Pos oi ken Paste as New Image Command Crop Command _ m Fill Command a i EE KT Select All Command aa Deselect Command Figure 8 Edit Menu Undo Command The Undo Windows Ctrl Z Macintosh Cmd Z command reverses the most recent operation performed on the image in the active image window Redo Command Windows only The Redo Windows Ctrl Y command re applies the previously undone action Cut Command The Cut Windows Ctrl X Macintosh Cmd X command removes the selected area from the active image wind
63. es located in the Studio Lite Status Bar Windows or in the Main Toolbar Macintosh To view pixel coordinates within an image You may position your mouse pointer over any portion of the active image window To view the X and Y axis coordinates for a particular pixel refer to the Mouse Pointer Coordinates located in the Studio Lite Status Bar Windows or in the Main Toolbar Macintosh Instruction Manual 137 Studio Lite 138 Viewing Informational Windows You may view information about the active image the Studio Lite application or the host computer system To view information about the Studio Lite application 1 Windows Click the Help menu Macintosh Click the Apple menu 2 Select the About Studio Lite command A splash screen containing current version number and copyright information will be displayed To view information about the host computer system 1 Windows Click the View menu Macintosh Click the Apple menu 2 Windows Select the System Info command Macintosh Select the Apple System Profiler menu item The System Information dialog box containing CPU Memory Operating System and Disk Information will be displayed To view information about the active image Method A 1 Click the View menu 2 Select the Image Info command Application Suite Viewing Informational Windows Studio Lite cont The Image Information dialog box containing file size bit depth and p
64. etween sessions and Windows sometimes uses it for temporary storage 104 Opening Existing Images cont will bring up the Open dialog box 2 Repeat steps 3 to 5 above Method D Windows Using your mouse drag one or more existing image file icons from the Windows file system and drop them directly within the Studio Lite application window Macintosh Using your mouse drag one or more existing image file icons from the Finder and let go of the mouse button when the arrow cursor is directly over the Studio Lite application icon and the icon becomes highlighted Method E Windows only Studio Lite provides shortcuts for up to the last four 4 previously opened image files To open one of these images do this in the File menu select the desired image from the list of recently opened images Using one of the methods A to E will result in the selected image appearing in a new image window If the file does not open the file format may not be supported or the file may be damaged Studio Lite provides shortcuts for up to the last four 4 previously opened image files To open one of these images 1 Click on the File menu 2 Select the desired image from the recently opened images list by clicking once directly on the filename The selected image should appear in a new image window If the file does not open the file format may not be supported or the file may be damaged Studio Lite Closing an Image Window
65. ew region of the preview Current Focus Level The Current Focus Level numerically or graphically displays the present level of focus quality for the region currently specified by Viewfinder Lite Windows Studio Lite Macintosh Focus Toolbar cont Viewfinder Lite the Focusing Target Maximum focus is achieved as the Current Focus Level approaches the Optimum Focus Level Optimum Focus Level The Optimum Focus Level numerically or graphically indicates the highest level of focus quality achieved up to the present time for the region currently specified by the Focusing Target The objective is to maximize the number which represents this level Focusing Target The Focusing Target may be placed on a region of the subject within the Viewfinder Lite image window that you wish to focus on It constantly displays the edge gradient of the region and is most suitable for use on areas of high contrast Used in conjunction with the Focus Indicator this tool assists in the focusing process Instruction Manual 97 6 Studio Lite Studio Lite d yl Studio Lite Version 1 0 SIISE Instruction Manual 99 Studio Lite Taskbar The Taskbar is the bar on the Windows desktop that includes the Start button Buttons representing programs currently running on your computer appear on this bar 100 Running the Application Windows The Studio Lite application allows for the editing and analysis of images captured
66. fined sensitivity settings High sensitivity settings may amplify unwanted noise in the image In general it is recommended that a sensitivity level of 100 be selected If the ISO sensitivity value is increased the noise level will also increase Viewfinder Lite Windows Studio Lite Macintosh Using Online Help Viewfinder Lite Viewfinder Lite offers the help topic documentation in online help including all of the information provided in this User s Manual To access online help 1 Click on the Help menu 2 Windows Select the Help Topics command Macintosh Select the Studio Lite Help menu item Online help is searchable by topic or keyword Instruction Manual 61 Viewfinder Lite 62 Using the Focusing Tool The Viewfinder Lite application offers a focusing tool which is designed to assist you in the process of focusing on a stationary subject Windows To view the Focus toolbar 1 Click on the Options menu 2 Place a checkmark next to the Focus Toolbar option Macintosh The Focus On Off and Focus Reset buttons are always visible To activate the Focus Indicator Method A 1 Click on the Viewfinder menu 2 Select the Focus On Off command Method B Click on the Focus On Off button located in the Focus toolbar To focus on a camera subject with the assistance of the focusing tool 1 First try to achieve the highest level of focus manually to bring the camera into rough f
67. flected in both the preview as well as the captured image Viewfinder Lite Windows Studio Lite Macintosh Viewfinder Menu cont Viewfinder Lite One Push The One Push white balance setting calculates the white balance based on the entire camera scene allowing the user to calibrate the Red Green and Blue RGB color correction coefficients by pointing the camera at a white target or light source The calibration is performed immediately upon selecting this option The coefficients stay fixed for all subsequent captures This setting allows accurate adjustments for applications in which the scenes remain relatively static and unchanged These settings are reflected in both the preview as well as the captured image Black Balance On Off Command The Black Balance On Off command switches between the activation and deactivation of the Black Balance feature of the Viewfinder Lite application When selected the Black Balance On Off button in the Capture toolbar is automatically activated allowing to you calibrate the black balancing by specifying any arbitrary region in the live image that is to be defined as the standard for black Once this region is specified the black balancing is automatically updated to provide optimum color reproduction This feature should be selected when viewing images which have discernable regions of true black When this command is not selected the black balancing feature is disabled These se
68. for example electrical equipment that is being switched on or off Also remember to use these chemicals only in a well ventilated room Parts other than the glass components should be cleaned by wiping with a clean cloth Do not use organic solvents to remove major stains Use a soft cloth slightly moistened with a neutral detergent solution To avoid any degradation in performance do not attempt to disassemble or modify any part of the system Table of Contents 1 Installing the HardWar8 ooooooncocccncnnnccncncccnnccnnnonnnccnononccnnnnnnenenennnnnes 9 2 Installing the Software ON WiNdOWS ooooomcoconcnnnnnnnnnnnannnnnnnnnnnnas 13 Installing the Device DriverS eeccceeseeeeeeeeeeeeeeseeeeeeeeeeeeeseeeeeeeees 13 Installing the Application icon 16 3 Installing the Software on MacintoSh oococncnnnnnnnnnnnnnnnnananannnas 21 Using the Installer soii ii as 21 4 Capturing Procedure tice ccc ssscccssesceceseseeceesutepcccccccevadentbetteeccesetee 27 5 Viewfinder Lite Windows Studio Lite Macintosh The Basi ui painta annaa aia aeae n neiuna nadasa naana du ndin aiaa 29 Running the Viewfinder Lite Application Wmdows 30 Running the Studio Lite Application Macintoeh 31 Viewing the CEET 32 Capturing Images iii dd naval 34 Selecting the Viewfinder Mode 36 Selecting the Capture Hesoluton anar cnn 38 Quitting the Viewfinder Lite Applcaton 39 Working Within the Viewfinder Lite Application Wimdow 41 Manipulati
69. ght mouse button 4 Select the Cut command The selected area of the image will be removed and replaced by the current background color The deleted image data is stored in the clipboard and will be available for use by Studio Lite or other Windows applications until the clipboard data is deleted or replaced by other data To copy all or a portion of the active image to the clipboard Method A 1 Click once on the desired image to make it the active image window 2 Using the Marquee tool specify a region of interest representing the portion of the image that you would like to copy 3 Click on the Edit menu 4 Select the Copy command Studio Lite Editing and Retouching Images Studio Lite cont Method B 1 Click once on the desired image to make it the active image window 2 Using the Marquee tool specify a region of interest representing the portion of the image that you would like to copy 3 Click the Copy button located in the Studio Lite Toolbar Method C 1 Click once on the desired image to make it the active image window 2 Using the Marquee tool specify a region of interest representing the portion of the image that you would like to copy 3 Windows Use the following keyboard shortcut Ctrl C Macintosh Use the following keyboard equivalent Cmd C Method D 1 Click once on the desired image to make it the active image window 2 Using the Marquee tool specify a r
70. he Exit command Method B Click once on the x button located at the far right of the Studio Lite application window s title bar Method C Double click the Control Menu icon located at the far left of the Studio Lite application window s title bar Method D 1 Click once on the Control Menu icon located at the far left of the Studio Lite application window s title bar 2 Select the Close command Method E Use the following keyboard shortcut Alt F4 You may be prompted to save any images which have not been saved or that have been altered since the last save operation took place Studio Lite Editing and Retouching Images Studio Lite offers powerful tools which simplify the editing and retouching of captured images Available operations include cut copy paste crop clear and fill functions Most operations can only be performed on portions of the image which lie within the visible image window Parts of the image which lie beyond this area may be repositioned using the Pan tool or Windows scroll bars Images captured by 48 bits can be performed only with the crop function To cut all or a portion of the active image to the clipboard Method A 1 Click once on the desired image to make it the active image window 2 Using the Marquee tool specify a region of interest representing the portion of the image that you would like to cut 3 Click on the Edit menu 4 Select the Cut command Method B
71. he Zoom Out button 5 Click the Close button to exit the preview mode and return to the standard Studio Lite application window To Print an Image Method A 1 Click once on the desired image to make it the active image window H you would like to print only a portion of the selected image use the Marquee tool to select the desired region of interest 2 Click on the File menu 3 Select the Print command This will bring up the standard Windows or Macintosh Print dialog box 4 If more than one printer is configured on your system you have the option of selecting which printer you would like to print from To Studio Lite Printing an Image cont Studio Lite select the printer click on the Printer pull down menu and highlight the desired printer 5 Windows only Click the Properties button to customize printer settings This will bring up the dialog box for the selected printer Available options are dependent on the particular printer and will vary from model to model 6 To print the image to a standard PostScript ps file click on the Print to File checkbox Windows or use the Destination popup menu Macintosh 7 Select the range of pages which you would like printed To print every page click on the All radio button To print a selected range of pages click on the Pages radio button and enter the range in the From and To boxes Windows only To print only the region of interest selected earlier
72. image data is pasted into the upper left corner of the active image window To denote a specific position for the pasted data specify a region of interest representing the destination Studio Lite Editing and Retouching Images Studio Lite cont area using the Marquee tool 3 Windows Use the following keyboard shortcut Ctrl V Macintosh Use the following keyboard equivalent Cmd V Method D 1 Click once on the desired image to make it the active image window 2 By default the image data is pasted into the upper left corner of the active image window To denote a specific position for the pasted data specify a region of interest representing the destination area using the Marquee tool 3 Click the Edit menu 4 Select the Paste command If no region of interest is specified as the destination area the image data appears at full size in the upper left corner of the active image window Otherwise the pasted image data is resized to fit within the specified area Valid image data must be present in the Windows Clipboard for the Paste command to be available To create a new image from the contents of the Windows Clipboard 1 Click the Edit menu 2 Select the Paste as New Image command A new image window appears containing the contents of the Clipboard Valid image data must be present in the Windows Clipboard for the Paste as New Image command to be available To crop an image Instructio
73. imited by the exposure time setting Instruction Manual 71 Viewfinder Lite 72 Viewfinder Menu cont Capture Size Command The Capture Size command allows for the selection of one of three image capture resolutions 640x480 1392x1040 and 2776x2074 Focus On Off Command The Focus On Off command switches between activation and deactivation of the Focusing Target and Focus Indicator When selected the Focus Indicator becomes enabled displaying a dynamic indication of the current focus level for the area specified by the Focusing target When this command is not selected the Focus Indicator feature is disabled Focus Reset Command The Focus Reset command sets the Optimum Focus Level to the Current Focus Level and is useful when you would like to calibrate the Focus Indicator on a new region of the preview White Balance Command The White Balance command allows for the selection of one of three different white balance modes Off Auto and One Push Off The Off setting disables all white balancing functions No white balance operations will be performed in either the preview or captured image Auto The Auto white balance setting allows the user to calibrate the white balancing by specifying any arbitrary region in the live image that is to be defined as the standard for white This feature should be selected when viewing images which have discernable regions of true white These settings are re
74. ion Manual 145 6 Studio Lite Studio Lite El Ip Ys pm ar ade jejeje aeigplei eil El AA E 3249 9194 ech Instruction Manual 47 Studio Lite 148 Title Bar Windows Only The strip across the top of the Studio Lite application window is called the Title Bar Figure 2 The Studio Lite program name icon and current filename are displayed along with buttons which represent functions and menus that contain commands Control Menu Close Studio Lite Button Maximize Button Minimize Button Close Button Figure 2 Title Bar Control Menu Close Studio Lite Button The Studio Lite icon displayed at the upper left corner of the Studio Lite application window represents the Control Menu Close Studio Lite button A single mouse click of this button displays the Control menu Double clicking this button closes the Studio Lite application window and quits the program Minimize Button Clicking once on the Minimize button reduces the Studio Lite application window to an icon on the Windows taskbar Maximize Button Clicking once on the Maximize button increases the size of the Studio Lite application window to fill the entire Windows desktop Close Button Clicking once on the Close button closes the Studio Lite application window and quits the program Application Suite Control Menu Windows Only Studio Lite The Control menu Figure 3 is comprised of six commands which control the physical aspe
75. ion as needed If you cannot solve the problem after checking the entire list please contact your local dealer for assistance Problem Cause Remedy The hand switch does not work The hand switch cable is not connected to the PCI interface card the PCI interface card Connect the hand switch cable properly to Images are not The computer is currently capturing or processing an image captured images are displayed Release the EXPOSE button after previous processed and Free disk space on Hard Disk Drive is not enough Windows only Make more than 100MB of free disk space on Hard Disk Drive The images are not in focus The microscope is not focused properly Adjust the focus correctly with the fine focus adjustment knob The aperture iris diaphragm of the condenser is open too wide Close the aperture iris diaphragm a little The field iris diaphragm is not set properly Close the field iris diaphragm until the image circumscribes the field of view Lens components of the microscope are contaminated or the cover glass on the front side of the camera is stained Clean the objective lens photography lens condenser and or window lens or clean the cover glass on the front side of the camera if the cover glass is stained The images are too dark or too bright AE lock Exposure Adjust BF and or FL are not set properly
76. it opge PETT Careri Let Figure 9a Settings Dialog Box User Preset Tab Macintosh Save Button The Save button brings up the Save Settings dialog box which allows you to save current Viewfinder Lite capture settings i e capture resolution white black balance calibrations etc so that they may be re applied at a later time The filename representing the currently saved settings is displayed in the dialog box 82 Viewfinder Lite Windows Studio Lite Macintosh Options Menu cont Viewfinder Lite Load Button The Load button brings up the Load Settings dialog box which allows you to import previously saved Viewfinder Lite capture settings i e capture resolution white black balance calibrations etc The filename representing the currently loaded settings is displayed in the dialog box Rotate 180 Command The Rotate 180 command rotates the live image in the Viewfinder Lite image window by a half turn 180 This change is reflected in the captured image as well Mirror Command The Mirror command flips the live image in the Viewfinder Lite image window horizontally along the vertical Y axis This change is reflected in the captured image as well Flip Command The Flip command flips the live image in the Viewfinder Lite image window vertically along the horizontal X axis This change is reflected in the captured image as well 16 Bits Channel Option When s
77. ith buttons which represent functions and menus that contain commands Control Menu Close Viewfinder Lite Button Maximize Button AL LH Minimize Button Close Button Figure 2 Title Bar Control Menu Close Viewfinder Lite Button The Viewfinder Lite icon displayed at the upper left corner of the Viewfinder Lite application window represents the Control Menu Close Viewfinder Lite button A single mouse click of this button displays the Control menu Double clicking this button closes the Viewfinder Lite application window and quits the program Minimize Button Clicking once on the Minimize button reduces the Viewfinder Lite application window to an icon on the Windows taskbar Maximize Button Clicking once on the Maximize button increases the size of the Viewfinder Lite application window to fill the entire Windows desktop Close Button Clicking once on the Close button closes the Viewfinder Lite application window and quits the program Viewfinder Lite Windows Studio Lite Macintosh Control Menu Windows Only Viewfinder Lite The Control menu Figure 3 is comprised of six commands which control the physical aspects of the Viewfinder Lite application window Restore Move Size Minimize Maximize and Close Restore Command a 4 Move Command Size ZE Minimize Command Maximize Command Close Command Figure 3 Control Menu Restore Command The Restore command returns the Vie
78. ixel dimension information will be displayed Instruction Manual 139 Studio Lite 140 Choosing the Foreground Color Windows Studio Lite uses the Foreground Color for paint operations and stroke selections using the Pencil Brush Line Rectangle and Ellipse tools The current Foreground Color is displayed in the lower right corner of the Studio Lite application window along with the current Background Color To select the Foreground Color Method A 1 Click on the Options menu 2 Select the Pen 8 Brush Color command This will bring up the standard Windows Color dialog box 3 A list of 48 available colors are displayed Click once on the desired color 4 Click OK to apply the change Method B 1 Double click on the Foreground Color displayed in the lower right corner of the Studio Lite application window This will bring up the standard Windows Color dialog box 2 Repeat steps 3 and 4 above To select a custom Foreground Color Method A 1 Click on the Options menu 2 Select the Pen 8 Brush Color command This will bring up the standard Windows Color dialog box Application Suite Choosing the Foreground Color Studio Lite cont 3 Press the Define Custom Colors button 4 Specify a custom color by entering either Hue Saturation and Luminance values or values for the Red Green and Blue color channels 5 Press the Add to Custom Color button 6 The specified color will be displaye
79. k on the button representing the desired Viewfinder Lite mode Fast Zoom or Full in the Options toolbar While these three preview modes allow for varying degrees of resolution image detail and frame rate the resultant final captured image will be identical regardless of the Viewfinder Lite mode used to preview the image Instruction Manual 37 Viewfinder Lite Resolution The degree of sharpness of a captured digital image A measure of the density of pixels on a screen resolution is measured by a matrix of two numbers which represent the number of pixels available across and down the screen e g 640x480 For example the VGA resolution of 640x480 means 640 dots pixels across each of the 480 lines The image file size is directly related to capture resolution the higher the resolution the larger the file For example an uncompressed 24 bit color image captured at VGA resolution will have a file size of approximately 900 kilobytes An uncompressed 24 bit color image captured at the maximum resolution of 2776x2074 will have a file size of approximately 16 3 megabytes 38 Selecting the Capture Resolution The Viewfinder Lite application offers three user selectable capture resolutions sizes 640x480 1392x1040 and 2776x2074 pixels Available capture resolutions are dependent upon the model of camera being used To select the capture resolution Method A 1 Click on the Viewfinder menu 2 Click
80. kbox switches between the activation and deactivation of the Black Balance feature of the Viewfinder Lite application When selected this feature allows the user to calibrate the black balancing by specifying any arbitrary region in the live Instruction Manual 77 Viewfinder Lite 78 Options Menu cont image that is to be defined as the standard for black Once this region is specified the black balancing is automatically updated to provide optimum color reproduction This feature should be selected when viewing images which have discernable regions of true black When this command is not selected the black balancing feature is disabled These settings are reflected in both the preview as well as the captured image Show Balance Rectangle Checkboxes The Show Balance Rectangle checkboxes toggle between the displaying and hiding of the balance rectangles which specify the calibration regions for the Auto White Balance and Black Balance operations When selected the selected regions are displayed in the Viewfinder Lite image window When this feature is not selected the balance rectangles are hidden from view Level Adjustment Tab The Level Adjustment tab Figure 8 allows you to perform dynamic histogram adjustments to the preview displayed in the Viewfinder Lite image window Shadow Gamma and Highlight values may be specified for either the individual color channels Red Green or Blue or the composite RGB cha
81. l appear 3 Click on Viewfinder Lite The Viewfinder Lite application appears with the logo displayed in the image window Viewfinder Lite Windows Studio Lite Macintosh Running the Studio Lite Viewfinder Application Macintosh Lite Launching the Studio Lite application is the first step in acquiring images with the digital camera system To run the Studio Lite application on Macintosh 1 Find the folder where you had the Installer place the Studio Lite files 2 Double click on the application icon Studio Lite The Studio Lite application appears with the logo displayed in the preview window Instruction Manual 31 Viewfinder Lite 32 Viewing the Preview Much like the optical viewfinder found on most standard film cameras the Viewfinder Lite application presents a live through the lens view of the camera s subject This allows for the framing and focusing of a subject before capturing the final image To start the preview Method A 1 Click on the Viewfinder menu 2 Select the Start Preview command Method B Windows Use the following keyboard shortcut Alt S Macintosh Use the following keyboard equivalent Cmd J Method C Click the Preview On Off button located in the Capture toolbar To stop the preview Method A 1 Click on the Viewfinder menu 2 De select the Start Preview command Method B Windows Use the following keyboard shortcut Alt S Macintosh
82. l as the Cut and Clear commands Double clicking on the Background Color will cause the Color dialog box to open allowing you to select a custom color On Mac OS machines these last four functions are placed on the right hand end of the Toolbar Studio Lite 7 TWAIN Viewfinder Lite Windows TWAIN Only Viewfinder Lite A A 1 CT ARES IAS SER ER Bel AG eee ino jdwex4 k Instruction Manual 75 TWAIN Viewfinder Lite The TWAIN Viewfinder Lite application is functionally and visually identical to the standard Viewfinder Lite application For more detailed information on using TWAIN Viewfinder Lite please refer to the instructions beginning on page 19 176 Using TWAIN Viewfinder Lite Windows Only The TWAIN Viewfinder Lite application provides an interface between popular imaging or image analysis programs and your camera system Functionally and visually identical to the standard Viewfinder Lite application the TWAIN interface allows you to capture images directly from your camera to most TWAIN compliant application programs Below is an illustrated example of accessing the TWAIN Viewfinder Lite interface from the popular third party image editing program Adobe Photoshop 1 Start the desired third party TWAIN compliant application Figure 1 BE 8 mmm eg op s be mp Figure 1 Run Third Party TWAIN Compliant Application 2 Select the correct TWAIN source device
83. l be opened by this command Full Screen Button The Full Screen button enlarges the active image window to occupy the entire screen Print Button The Print button brings up the Print dialog box which allows you to select options for printing the image in the active image window with any accessible printer Macintosh only On the Macintosh four additional functions are located on the right hand end of the Toolbar On Windows machines these four functions are placed on the Windows Status Bar Mouse Pointer Coordinates The Mouse Pointer Coordinates display the horizontal X axis and vertical Y axis pixel coordinates for the current mouse pointer position Color Channel Values The Color Channel Values display the Red R Green G and Blue B color channel values for the current mouse pointer pixel position Foreground Color Double clicking on the Foreground Color patch will cause the Color Studio Lite Toolbar cont Studio Lite dialog box to open allowing you to select a custom color The Foreground Color is used with the Fill Color option Background Color The Background Color displays the current color selected for the background pertaining to the Cut and Clear commands Double clicking on the Background Color will cause the Color dialog box to open allowing you to select a custom color Instruction Manual 171 Studio Lite 172 Toolbox The Toolbox Figure 20 amp 20a provides
84. level of the preview image in the Viewfinder Lite image window Dragging to the left decreases the value dragging to the right increases it Values can range from 0 to 255 This setting is represented graphically on the histogram display and reflected in the captured image Gamma Slider The Gamma Slider adjusts the gamma level of the preview image in the Viewfinder Lite image window Dragging to the left decreases the value dragging to the right increases it Values can range from O to 5 This setting is represented graphically on the histogram display and reflected in the captured image Highlight Slider The Highlight Slider adjusts the highlight level of the preview image in the Viewfinder Lite image window Dragging to the left decreases the value dragging to the right increases it Values can range from 0 to 255 This setting is represented graphically on the histogram display and reflected in the captured image Macintosh There are three marker triangles below the histogram instead of the three sliders that the Windows version has The meaning and use of these marker triangles however matches that of Windows sliders Thus the dark left hand marker corresponds to the Shadow slider the gray middle marker to the Gamma slider and Viewfinder Lite Windows Studio Lite Macintosh Options Menu cont Viewfinder Lite the white right hand marker to the Highlight slider See the Windows text for further de
85. lication window s title bar 2 Select the Close command Method E Use the following keyboard shortcut Alt F4 For Macintosh users Method A Select Quit in the File menu Instruction Manual Viewfinder Lite 39 Viewfinder Quitting the Viewfinder Lite Lite Application cont Method B Use the keyboard equivalent Cmd Q 40 Viewfinder Lite Windows Studio Lite Macintosh Working Within the Viewfinder Lite Application Window Viewfinder Lite allows for the customization of the viewing properties of the Viewfinder Lite application window For Windows users To display or hide the Capture toolbar 1 Click the Options menu 2 To display the Capture toolbar place a checkmark next to the Capture Toolbar option To hide the Capture toolbar remove the checkmark To display or hide the Options toolbar 1 Click the Options menu 2 To display the Options toolbar place a checkmark next to the Options Toolbar option To hide the Options toolbar remove the checkmark To display or hide the Focus toolbar 1 Click the Options menu 2 To display the Focus toolbar place a checkmark next to the Focus Toolbar option To hide the Focus toolbar remove the checkmark To display or hide the Status Bar 1 Click the Options menu 2 To display the Status Bar place a checkmark next to the Status Bar option To hide the Status Bar remove the checkmark Instruction Man
86. n Manual 123 Studio Lite 124 Editing and Retouching Images cont Method A 1 Using the Marquee tool specify a region of interest representing the portion of the image that you would like to keep 2 Click the Edit menu 3 Select the Crop command To discard a portion of the active image Method A 1 Click once on the desired image to make it the active image window 2 Using the Marquee tool specify a region of interest representing the portion of the image that you would like to discard 3 Click on the Edit menu 4 Select the Clear command Method B Windows only 1 Click once on the desired image to make it the active image window 2 Using the Marquee tool specify a region of interest representing the portion of the image that you would like to discard 3 Use the following keyboard shortcut Delete To fill a portion of the active image with a specified color Method A Studio Lite Editing and Retouching Images Studio Lite cont 1 Click once on the desired image to make it the active image window 2 Using the Marquee tool specify a region of interest representing the portion of the image that you would like to fill 3 Click on the Edit menu 4 Select the Fill command The selected area of the image will be filled by the current Pen amp Brush Color Instruction Manual 125 Studio Lite Editing and Retouching Images cont 126 Studio Lite Specif
87. n open previously saved images in a variety of image file formats and have multiple images opened simultaneously The following image file format types are supported by Studio Lite s Open command Bitmap FlashPix JPEG LeadTools Mac PICT PCX Photo CD PNG Targa TIFF and Windows Metafile To open an existing image file Method A 1 Click on the File menu 2 Select the Open command This will bring up the Open dialog box 3 Windows only Specify a file format In the Files of Type dialog box choose an individual image format type to display only files saved in that particular format Select Images to display all supported image files in the selected folder Choose All Files to display all of the files including those with unsupported file formats in the selected directory 4 Select the file 5 Double click the file name or highlight it and click the Open button Method B 1 Windows Use the following keyboard shortcut Ctrl O Macintosh Use the following keyboard equivalent Cmd O This will bring up the Open dialog box 2 Repeat steps 3 to 5 above Method C 1 Click the Open button located in the Studio Lite Toolbar This Instruction Manual Studio Lite Bitmap Bitmap BMP is the standard Windows image format on DOS and Windows compatible computers FlashPix The FlashPix FPX format is designed to speed the transfer and display of large high resolution files in application
88. nce on the desired image to make it the active image window 2 Windows Use the following keyboard shortcut Method C 1 Click once on the desired image to make it the active image window 2 Click on the Zoom button located in the Studio Lite Toolbox When placed over the active image the mouse pointer will be designated by a magnifying glass 3 Click any area within the active image area which you would like to magnify Each click magnifies the image to the next preset percentage centering the display around the point you click To zoom out of an image Application Suite Magnifying and Reducing the View Studio Lite cont Method A 1 Click once on the desired image to make it the active image window 2 Click the View menu 3 Select the Zoom Out command Method B 1 Click once on the desired image to make it the active image window 2 Windows Use the following keyboard shortcut Method C 1 Click once on the desired image to make it the active image window 2 Click on the Zoom button located in the Studio Lite Toolbox When placed over the active image the mouse pointer will be designated by a magnifying glass 3 Hold down the Shift key and click any area within the active image area which you would like to reduce Each click reduces the image to the next preset percentage centering the display around the point you click To magnify or reduce to a specified percentage 1 Click on
89. ndard for compressing still images that is becoming very popular due to its high compression capability Using discrete cosine transform it provides 107 Studio Lite lossy compression you lose some data from the original image with ratios up to 100 1 and higher It depends on the image but ratios of 10 1 to 20 1 may provide little noticeable loss The more the loss can be tolerated the more the image can be compressed Compression is achieved by dividing the picture into tiny pixel blocks which are halved over and over until the ratio is achieved Mac PICT The PICT PCT format is the primary Macintosh graphics file format lt holds QuickDraw vector images bitmapped images and text and is the Mac counterpart to the Windows Metafile WMF format TIFF The Tagged Image File Format TIF is used to exchange files between applications and computer platforms A widely used bitmapped graphics file format developed by Aldus and Microsoft TIFF handles monochrome grayscale 8 and 24 bit color Image File Compression Compression is the encoding of data to take up less storage space Digital data is compressed by finding repeatable patterns of binary 108 Saving Images cont window 2 Click on the File menu 3 Select the Save command The Save As dialog box should appear 4 Specify a file format in which you would like the image saved In the Save as Type pull down list Windows
90. ndows cont 7 You will be presented with the Characteristics Data Installation dialog box Figure 6 Select from one of the two installation options and click the Next button to complete the software installation process Install Custom Characteristics Data The recommended choice this option will prompt you for the floppy disk supplied with your camera containing characteristics data which was generated specifically for your specific camera unit Install Default Characteristics Data from the CD ROM This option will install a non specific standard set of characteristics data Select this option if you do not have access to the original floppy diskette supplied with your camera kesiputg ate ria E Ste ali dl ue Dic Can cd peee h pa ap ir pan rrr kb verbe band cs rad Bai Aer he E den 9 an imee Te mkrn knj vs md mpre fe fa s dai Wei Liam Leg E im ma TASA Pem r ge Demen Dar hopo had in ie oh E rd db Ciro Den Fe ihe COP Instruction Manual 19 3 Installing the Software on Installing the Macintosh Software on Macintosh The Studio Lite software should be installed after the successful installation of the camera hardware Turn on your computer and follow the instructions outlined below to install the Studio Lite software and device driver extension under the MacOS operating system The software package is available on the Setup CD ROM the Studio Lite software and device driver extension will be installed at
91. ng Images 43 Adjusting the Color Balance 45 elei WE 51 Using Automatic EXPOSU oooccccoococcccconoconcnccnanoncnnncnnnonnncn nano nn carencia nn nr rra nn nnnr nana 56 Using the Spot Meter 58 Using Manual Exvposure cnn rc nano n nn anna aA 59 Adjusting Camera Gensitlvity cnn non cnn rcnnnnnrnnnncc 60 Using Online Hel pis aiii di it 61 Using the FOCUSING Tool 62 ETGEN 65 Title Bar Windows On 66 Control Menu Windows On 67 Menus BD cia AA A eee alee ete SEENEN 69 Viewfinder Menmi orotar e A A E E a E 70 Options Mel ida A IS da ea ae 74 Help Menl act tia 85 Capture Tolva AAA A NEE Ed E 87 Exposure Todavia 91 Options Toolbar srna A naan 93 Focus Toolbar ini ca 96 6 Studio Lite The TN 99 Running the Application conan nonnnonano cnn rr nana n cnn rnnnn nn 100 CAEN ce eee ee sere eeeeeeeeeesaeseaeesaeesaeeseeeeseeseeeenereneaeeeaees 101 Selecting the Active Image Wimdow nara 102 Opening Existing Images 103 Closing an Image Wimdow cee eee eee seen cece sees eee eeaee sees taeeseeeseneeneaeeneees 105 Saving IMAGES EE 107 Correcting Mistakes AA 111 Pining AMADO di A Neng 113 Switching to Viewfinder anar rancia rra 117 Quitting the Studio Lite Application Windows Only oooonncccnnnnnnnonncccnncccnnnccccnnos 118 Editing and Retouching Images 119 Specifying a Region of Interest cnn nn nananonnnnos 127 Magnifying and Reducing the View 130 Working Within the Studio Lite Application Window Windows Only
92. nnel The current histogram for the live image being previewed in the Viewfinder Lite image window is represented graphically in the center of the dialog box Viewfinder Lite allows you to save current level adjustment settings as well as load any previously saved settings Viewfinder Lite Windows Studio Lite Macintosh Options Menu cont Viewfinder Lite Coto Been L cgdk rgeg bees Ferre Channel Pull Down List e EE EE Load Button Input Level begiers f y po Save Button Histogram ee Update Button Grah Fit Reset Button Shadow Slider atara AAA Gamma Slider Highlight 444 Slider Figure 8 Setting Dialog Box Level Adjustment Tab O O Calar nr A Lemel Ai beleet i Weer Feel ee a Figure 8a Settings Dialog Box Level Adjustment Panel Macintosh Channel Pull Down List or Popup Menu The Channel pull down list or popup menu allows you to select the color channel s on which you would like the level adjustment operations performed You may select any of the individual color channels Red Green or Blue or the composite RGB channel Instruction Manual 79 Viewfinder Lite 80 Options Menu cont Input Level The Input Level displays three text boxes indicating from left to right numerical input values for the Shadow Gamma and Highlight settings respectively Windows Shadow Slider The Shadow Slider adjusts the shadow
93. oard equivalent Cmd W will also work To close all the image windows hold the Option key down before using one of the three methods for single windows Studio Lite Saving Images Studio Lite supports a variety of file formats to suit a wide range of output needs Using Studio Lite s save commands you can save your image to any of the following formats JPEG Bitmap TIFF TIFF compressed Mac PICT and FlashPix Windows only JPEG When saving in Joint Photographic Experts Group JPEG format Studio Lite allows you to specify the image quality and compression level To specify image quality and compression enter a Quality value between 1 and 100 or drag the slider bar Higher quality images use less compression and more disk space than lower quality images In most cases the maximum quality option produces a result indistinguishable from the original The following color depths are available in JPEG mode 256 Grays 8 bits and 16 million colors 24 bits Bitmap Studio Lite allows for the selection of the following color depths when saving images in the standard Windows bitmap format Monochrome 1 bit 16 Colors 4 bits 256 Colors 8 bits and 16 million colors 24 bits TIFF Studio Lite allows for the selection of the following color depths when saving images in the Tagged Image File format Monochrome 1 bit 16 Colors 4 bits 256 Colors 8 bits 16 million colors 24 bits and 281 trillion colors 48 bits
94. ocus 2 Click once using the left mouse button on any region within the live image window to place the Focusing Target on the area that you wish to focus on Selecting an area of high contrast i e a distinct edge will optimize the accuracy of the final focus Viewfinder Lite Windows Studio Lite Macintosh Using the Focusing Tool cont Viewfinder Lite 3 Observe the Current Focus Level Indicator in the Focus toolbar The Current Focus Level Indicator numerically or graphically displays the present level of focus quality for the region currently specified by the Focusing Target Slowly turn the focusing knob of the microscope or macro lens in one direction until the Current Focus Level Indicator begins to steadily decline 4 Slowly turn the focusing knob of the microscope or macro lens in the direction opposite to the one you chose in Step 3 You will notice that the Current Focus Level Indicator will begin to steadily increase Continue turning until the Current Focus Level Indicator begins to steadily decline again The Optimum Focus Level Indicator should now be at its maximum value The Optimum Focus Level numerically or graphically indicates the highest level of focus quality achieved up to the present time for the region currently specified by the Focusing Target The objective is to maximize the number which represents this level 5 Slowly turn the focusing knob of the microscope or macro lens in the direction opp
95. omatic This will activate automatic exposure mode and enable the Spot and Exposure Adjust pull down lists or popup menus as well as the AE Lock controls The spot meter will be displayed in the center of the Viewfinder Lite image window as a square region defined by a yellow border There may be circumstances when a scene is under or over exposed or you may wish to make slight adjustments to the exposure based on your preferences To incrementally adjust the automatic exposure 1 Click the Exposure Adjust pull down list or popup menu located in the Exposure toolbar 2 Select an increment by which you wish to increase or decrease the exposure time Two indicators Over and Under provide feedback regarding whether the image is over exposed or under exposed To fix the exposure time at its current setting Method A 1 Click on the Viewfinder menu 2 Select the AE Lock command Viewfinder Lite Windows Studio Lite Macintosh Using Automatic Exposure cont Viewfinder Lite Method B Click on the AE lock button located in the Capture toolbar Method C Press the AE Lock button on the external handswitch optional With the AE Lock feature enabled the exposure time is no longer updated automatically and is fixed at its current value The Viewfinder Lite application includes predefined settings for the automatic exposure mode optimized for the unique conditions presented by brightfield and fluoresc
96. ommand Method B Windows Use the following keyboard shortcut Ctrl N Macintosh Use the following keyboard equivalent Cmd N Method C Click the New button located in the Studio Lite Toolbar If no valid image data is stored in the Clipboard the default dimensions for the new image window is 320x240 pixels Instruction Manual Studio Lite Windows Clipboard A reserved section of memory that is used as a temporary holding area when you copy and paste or cut and paste from one application to another After you copy or cut data from your application it is viewable in the clipboard If you cut data from an application and do not paste it into another and you do not save the clipboard s contents as a file the data will be lost upon exiting Windows or when the next copy or cut operation is performed 101 Studio Lite 102 Selecting the Active Image Window When multiple image windows are opened Studio Lite requires that an active image be selected to target operations to that specific image To specify the active image Method A Click once anywhere within the desired image window Method B Select the desired image in the Window menu The active image is brought to the foreground of the Studio Lite application window and a checkmark is placed next to the filename of the active image in the Open Windows list located in the Window menu Studio Lite Opening Existing Images In Studio Lite you ca
97. on iia Sharpness Option HO amp Ui uais Maes Figure 6 Options Menu iedom 1 CETT Segitegm Enter pat miar Fip i ra kkee press L Figure 6a Options Menu Macintosh Capture Toolbar Option Windows Only The Capture Toolbar option displays the Capture Toolbar when selected hiding it otherwise Options Toolbar Option Windows Only The Options Toolbar option displays the Options Toolbar when selected hiding it otherwise Focus Toolbar Option Windows Only The Focus Toolbar option displays the Focus Toolbar when Viewfinder Lite Windows Studio Lite Macintosh Options Menu cont selected hiding it otherwise Status Bar Option Windows Only The Status Bar option displays the Status Bar when selected hiding it otherwise Exposure Toolbar Option Windows Only The Exposure Toolbar option displays the Exposure Toolbar when selected hiding it otherwise Grayscale Command When selected the Grayscale command converts the preview image in the Viewfinder Lite image window from color to shades of gray This change is reflected in the captured image as well Settings Command The Settings command brings up the Settings property sheet or dialog box which allows you to customize the image processing functions of Viewfinder Lite with the following tabs Color Balance Level Adjustment and User Preset Settings Dialog Box
98. on reduction factor is recommended Please contact your microscope equipment supplier for specific details regarding adapters Most microscopes that accept camera attachments are trinocular in design allowing the camera to be attached to the center tube If your microscope is not trinocular it may still accept a camera if one of the two eyepieces allows for C mount conversion Refer to the documentation for your particular microscope 1 Fit the C mount adapter on your microscope Be sure that your coupler has parfocalizing capability allowing you to synchronize the Co h binocular image with the camera s GE viewfinder image ls Disconnect the cable from the gt camera before attempting to attach it to a microscope Excessive stress on the connector may cause damage to the camera or the cable Installing the Hardware 1 Installing the Hardware cont 2 Thread the camera onto the coupler and attach the interface cable to the camera Caution DO NOT overtighten the camera to the coupler Doing so may damage the camera Other Applications Some digital camera models feature a standard tripod screw mount which allows you to connect the camera to standard desktop copystands or camera tripods External Handswitch Optional The optional external handswitch see figure below provides an alternative control interface to the camera duplicating several basic functions of the Viewfinder Lite application AELOCK
99. osite to the one you chose in Step 4 or the same direction as Step 3 Continue turning until the Current Focus Level Indicator equals or is close as possible to the Optimum Focus Level This indicates that the subject has reached the Optimum Focus Level and is in focus If you experience difficulty matching the Current Focus Level Indicator to the Optimum Focus Level it may be necessary to reset the focusing tool and repeat the above steps To reset the focusing tool Method A 1 Click on the Viewfinder menu 2 Select the Focus Reset command Instruction Manual 63 Viewfinder Lite 64 Using the Focusing Tool cont Method B Click on the Focus Reset button located in the Focus toolbar To deactivate the Focus Indicator Method A 1 Click on the Viewfinder menu 2 Select the Focus On Off command Method B Click on the Focus On Off button located in the Focus toolbar Viewfinder Lite Windows Studio Lite Macintosh 5 Viewfinder Lite Viewfinder Lite Meadas pc Uer ld ka LE bd i aed jur Ed MS D e dd seh bass iist Aa Emi Tha IM fi esate 55 d T om nyema Capes Hee fr E sporas ho rem m i 99U9319J H Instruction Manual 65 Viewfinder Lite 66 Title Bar Windows Only The strip across the top of the Viewfinder Lite application window is called the Title Bar Figure 2 The Viewfinder Lite program name and icon are displayed along w
100. ow and places it in the clipboard Studio Lite fills the empty space with the current Background Color Copy Command The Copy Windows Ctrl C Macintosh Cmd C command copies the selected area to the clipboard without removing it from the active image window Application Suite Edit Menu cont Studio Lite Paste Command The Paste Windows Ctrl V Macintosh Cmd V command places the image data content in the clipboard into the active image window Paste as New Image Command The Paste as New Image command places image data that is currently in the clipboard into Studio Lite as a new image with its own image window Crop Command The Crop command allows you to remove an unwanted board around your image by deleting all parts of the image in the active image window that are not selected within a specified region of interest creating a smaller rectangular image that will retain the portion of the image you want to keep Clear Command The Clear Del command removes the selected highlighted area from the active image window filling the space with the current Background Color Use this command to delete information without affecting the clipboard Fill Command The Fill command fills the selected highlighted area within the active image window with the current Foreground Color Select All Command The Select All command Macintosh Cmd A selects the entire image in the active image window a
101. ox Instruction Manual 143 Studio Lite 144 Viewing Images Windows Only Studio Lite offers a wide range of options and tools for viewing images To open multiple views of the same image 1 Click once on the desired image to make it the active image window 2 Click the Window menu 3 Select the New Window command A duplicate of the active image is displayed in a new image window To display a cascading view of all opened image windows 1 Click the Window menu 2 Choose the Cascade command All opened image windows are displayed stacked and cascading from the top left to the bottom right of the Studio Lite application window To display a tiled view of all opened image windows 1 Click the Window menu 2 Choose the Tile command All opened image windows are displayed edge to edge in the Studio Lite application window To arrange all minimized image windows 1 Click the Window menu 2 Choose the Arrange Icons command Minimized image windows are displayed neatly along the bottom of the Studio Lite application window Studio Lite Using Online Help Studio Lite Studio Lite offers help topic documentation in online help including all of the information provided in this User s Manual To access online help 1 Click on the Help menu 2 Windows Select the Help Topics command Macintosh Select the Studio Help menu item Online help is searchable by topic or keyword Instruct
102. pecifying any arbitrary region in the live image that is to be defined as the standard for white This feature should be selected when viewing images which have discernable regions of true white These settings are reflected in both the preview as well as the captured image One Push White Balance The One Push White Balance setting calculates the white balance based on the entire camera scene allowing the user to calibrate the Red Green and Blue RGB color correction coefficients by pointing the camera at a white target or light source The calibration is performed immediately upon selecting this option The coefficients stay fixed for all subsequent captures This setting allows accurate adjustments for applications in which the scenes remain relatively static and unchanged These settings are reflected in both the preview as well as the captured image Manual Color Correction The Manual Color Correction setting offers full user control over the color correction settings Three separate sliders allow you to specify custom values for the Red Green and Blue color correction coefficients Dragging to the left decreases the level dragging to the right increases it The numbers above the slider display the numerical color channel values Values can range from 0 to 2 The default color channel values are 1 These settings are reflected in both the preview as well as the captured image Black Balance Checkbox The Black Balance chec
103. ption displays the Toolbar when checked while hiding it when unchecked Status Bar Option Windows Only The Status Bar F4 option displays the Status Bar when checked while hiding it when unchecked Toolbox Option Windows Only The Toolbox F5 option displays the Toolbox when checked while hiding it when unchecked System Info Command Windows Only The System Info command presents a dialog box Figure 11 with configuration information regarding the host computer system TA PE Pia Parar A ar fea Pira Sep LESCHE Veda teat ieee ZER CR Aras eh e REA ze Pata lat da Am Deb egen A ANA al Lp ZA ot Fae Specs iE H fee E CS Figure 11 System Information Dialog Box Application Suite View Menu cont Image Info Command The Image Info command presents a dialog box Figure 12 with detailed information about the active image Garma Fie Mis ESO TAIL cd Ti E map Los Kn Caen im podr A hij E Leet Hegd in eer Ce te leage irae Ire hame Ea bi ee eo Lara magr CERA baies Zaior Ub k ng Peters 124 Ei tishi ta pisri Hapi App peih ISCH Figure 12a Image Information Dialog Box Macintosh Refresh Command Windows Only The Refresh command refreshes the active image window Instruction Manual Studio Lite 163 Studio Lite 164 Window Menu Windows The Window menu Figure 16 controls the positioning of images and icons within the
104. rompting you to select the folder into which you would like to install the application files You may choose to accept the default name and location for the Studio Lite folder or to specify a custom path When you are finished click Install nia ci dr H 1 COR ramia Bet Int ee aya Htpuenp J Aug pa Y irie tirar Pro 3 m 5 u J Leva bin Lite fire Pr eb a italy Late Iris Figure A 5 Destination Folder Instruction Manual 23 Installing the Software on Macintosh Each camera is shipped with a CD ROM labeled Camera Data containing custom characteristics data generated by that particular camera unit This data allows for the calibration of capture settings for maximum performance 24 3 Installing the Software on Macintosh cont 7 You will be presented with the Characteristics Data Installation dialog box Select from one of the two installation options in the Figure A 6 and click the Next button to complete the software installation process E install custom Camera Data O Install default Camera Data Figure A 6 Camera Data Installation Window Install Custom Characteristics Data The recommended choice this option will prompt you for the CD ROM supplied with your camera containing characteristics data which was generated specifically for your specific camera unit Insert CD ROM labeled Camera Data into the CD ROM Drive Then click the OK button
105. rtically along the horizontal axis Method A 1 Click on the Options menu 2 Select the Flip command Method B Click the Flip button located in the Options toolbar To Sharpen the Image 1 Click on the Options menu 2 Select the Sharpness option 3 Select the desired level of sharpness Four distinct settings of the sharpening filter are available Off no sharpening will be applied Minimum Medium and Maximum each one applying the sharpening filter to a different degree These manipulations are reflected in both the preview the effect of Sharpening is previewed only in the Zoom Viewfinder Lite mode as well as the final capture Viewfinder Lite Windows Studio Lite Macintosh Adjusting the Color Balance Viewfinder Lite Viewfinder Lite offers a wide range of operations to optimize color reproduction and accuracy These operations include white balance and black balance as well as color correction To enable and calibrate One Push White Balance Method A 1 Click on the Options menu 2 Select the Settings command This will bring up the Settings property sheet or dialog box 3 Click the Color Balance tab 4 Point the camera at a white target for microscopy applications remove a slide place a blank slide on the microscope or move the slide so that a blank or white region of the specimen is positioned into the field of view 5 Select One Push White Balance 6 Click OK to clos
106. s that support the FlashPix technology JPEG The Joint Photographic Experts Group JPG format is an ISO ITU standard for compressing still images that is becoming very popular due to its high compression capability Using discrete cosine transform it provides lossy compression LeadTools The Lead Compressed CMP format results in smaller files and better image quality than industry standard formats Mac PICT The PICT PCT format is the primary Macintosh graphics file format and is the Mac counterpart to the Windows Metafile WMF format PCX The PCX format is commonly 103 Studio Lite used by IBM PC compatible computers This format is capable of achieving compression ratios of approximately 1 1 1 to 1 5 1 Photo CD A CD imaging system from Kodak that digitizes 35mm slides or negatives onto a CD ROM disc PNG A bitmapped graphics file format endorsed by the World Wide Web Consortium Targa The Targa TGA format is a bitmapped graphics file format developed by Truevision Inc TIFF The Tagged Image File Format TIF is used to exchange files between applications and computer platforms Windows Metafile The Windows Metafile WMF file format that holds vector graphics bitmaps and text The Windows Metafile is Windows preferred vector format since it contains actual Windows commands GDI calls to draw the images It is also used by programs to hold data b
107. s the region of interest Deselect Command The Deselect command nullifies the currently selected region of interest within the active image window Instruction Manual 159 Studio Lite 160 View Menu The View menu Figure 9 amp 9a allows you to control the viewing of images and customize the user interface by showing or hiding elements of the Studio Lite workplace Viewing options include Zoom In Zoom Out Zoom Factor Actual Pixels Fit in Window Fit to Screen Full Screen Edit View Toolbar Status Bar Toolbox System Info Image Info and Refresh ies Dm dos H KT H Zoom In Command Zoe jad Zoom Out Command Zoom Factor Command Zeche ee ae Actual Pixels Command Fit In Window Command Fi e jaia Fi ic B ajir Fit to Screen Command Full Screen Command Fado cf Edit View Command Toolbar Option e oka Toolbox Opti is Status Bar Option oolbox Option System Info Command Image Info Command Refresh Command Figure 9 View Menu conan de D Zem Dal k Jion faia Briugl Palu MA in Bini DA le kist en fab krss Image inie Figure 9a View Menu Macintosh Zoom In Command The Zoom In Windows command allows you to magnify the active image by selecting a zoom factor ranging from 200 to 800 Zoom Out Command The Zoom Out Windows command allows you to decrease the magnification of the active image by selecting a zoom
108. s which adjust the properties of the live image presented in the Viewfinder Lite image window as well as the captured image These include Rotate 180 Mirror Flip Toggle Grayscale Settings Brightfield Fluorescence Fast Zoom and Full Windows only The Options toolbar can be manually repositioned to any location on the Windows desktop using mouse drag and drop Double clicking on the upper border of the Options toolbar will return H to the Viewfinder Lite application window Settings Button Fluorescence Button Rotate 180 Button Flip ae Zoom Button deja ths oe Jar ole ai Mirror Button Fast Button Full Button Toggle Grayscale Button Brightfield Button Figure 14 Options Toolbar qu bl e En e ae Figure 14a Options Toolbar Controls Macintosh Rotate 180 Button The Rotate 180 button rotates the live image in the Viewfinder Lite image window by a half turn 180 This change is reflected in the captured image as well Mirror Button The Mirror button flips the live image in the Viewfinder Lite image window horizontally along the vertical Y axis This change is reflected in the captured image as well Flip Button The Flip button flips the live image in the Viewfinder Lite image window vertically along the horizontal X axis This change is reflected in the captured image as well Instruction Manual 93 Viewfinder Lite 94 Options Toolbar cont Toggl
109. se button at the starting point of the selection 7 Drag the mouse to encompass the desired region of interest 8 Release the mouse button Viewfinder Lite Windows Studio Lite Macintosh Adjusting the Color Balance cont Method B 1 Click on the Settings button located in the Options toolbar This will bring up the Settings property sheet or dialog 2 Repeat steps 3 through 8 above Method C 1 Click on the Viewfinder menu 2 Select the White Balance command and select Auto 3 Repeat steps 6 through 8 above Method D 1 Depress the Auto White Balance button located in the Capture toolbar 2 Repeat steps 6 through 8 above The specified region of interest will be bordered by a rectangular frame and the Auto White Balance will be calibrated according to the target white reference used To enable and calibrate Black Balance Method A 1 Click on the Options menu 2 Select the Settings command This will bring up the Settings property sheet or dialog box 3 Click the Color Balance tab 4 Select the Black Balance checkbox Instruction Manual Viewfinder Lite 47 Viewfinder Lite 48 Adjusting the Color Balance cont 5 Click OK to close the property sheet or dialog 6 To specify a reference black area within the Viewfinder Lite image window Click and hold the left mouse button at the starting point of the selection 7 Drag the mouse to encompass the de
110. sentation of the printed image will be displayed on the screen 4 Click on the Print button located at the top of the page This will bring up the standard Windows or Macintosh Print dialog box 5 Repeat steps 4 to 9 above By default Studio Lite formats the printed image to fill the print media while preserving the original aspect ratio Print quality and color reproduction are dependent on a number of factors including printer type i e dye sublimation laser ink jet etc printer resolution image resolution and proper monitor calibration Studio Lite Switching to Viewfinder Studio Lite Windows Studio Lite offers the capability of launching the Viewfinder Lite application from the Studio Lite application To run Viewfinder Lite Click the Viewfinder Lite button located in the Studio Lite Toolbar The Viewfinder Lite application window will then be displayed Macintosh The functionality of Studio and Viewfinder are integrated in one application on Macintosh At any time when there are image windows open the Macintosh user can switch to the Viewfinder window by 1 clicking on some part of the Viewfinder window itself 2 selecting it in the Window menu or 3 pressing the Viewfinder button on the Main Toolbar Instruction Manual 117 Studio Lite 118 Quitting the Studio Lite Application Windows Only To close the Studio Lite application Method A 1 Click on the File menu 2 Select t
111. side the selected region of interest To reposition a previously specified region of interest 1 Click on the Marquee button located in the Studio Lite Toolbox When placed over the active image the mouse pointer will be designated by a crosshair 2 Click and hold the mouse button Windows left hand button anywhere within the selected region of interest 3 Drag the region of interest to the desired position 4 Release the mouse button To resize a previously specified region of interest 1 Click on the Marquee button located in the Studio Lite Toolbox When placed over the active image the mouse pointer will be designated by a crosshair 2 Click and hold the mouse button Windows left hand button on a corner or side anchor of the rectangular frame of the region of interest Application Suite Specifying a Region of Interest cont 3 Drag the anchor to resize the region of interest as desired 4 Release the mouse button Instruction Manual Studio Lite 129 Studio Lite 130 Magnifying and Reducing the View Studio Lite allows you to magnify or reduce your view of an image using a variety of methods The current zoom percentage of a displayed image is displayed in the title bar of the image at all times To zoom in on an image Method A 1 Click once on the desired image to make it the active image window 2 Click the View menu 3 Select the Zoom In command Method B 1 Click o
112. sired region of interest 8 Release the mouse button Method B 1 Click on the Settings button located in the Options toolbar This will bring up the Settings property sheet or dialog box 2 Repeat steps 3 through 8 above Method C 1 Click on the Viewfinder menu 2 Place a checkmark next to the Black Balance On Off command 3 Repeat steps 6 through 8 above Method D 1 Depress the Black Balance On Off button located in the Capture toolbar 2 Repeat steps 6 through 8 above The specified region of interest will be bordered by a rectangular frame and the Black Balance will be calibrated according to the target black reference used To utilize the manual color correction feature Viewfinder Lite Windows Studio Lite Macintosh Adjusting the Color Balance cont Method A 1 Click on the Options menu 2 Select the Settings command This will bring up the Settings property sheet or dialog 3 Click the Color Balance tab 4 Select Manual Color Correction 5 Adjust the color balance slider bars for each of the separate color channels red green and blue to specify the desired color balance levels 6 Click the Apply button to apply the color correction settings 7 Click OK to close the property sheet or dialog box Method B 1 Click on the Settings button located in the Options toolbar This will bring up the Settings property sheet or dialog 2 Repeat steps 3 thro
113. table for positioning the subject rapid focusing and determining optimum brightness levels In Fast mode the previewed effect of the camera exposure time setting is Viewfinder Lite Windows Studio Lite Macintosh Options Toolbar cont Viewfinder Lite limited to approximately 1 4 second While the effect of longer exposure times will be reflected in the captured image the effect in the preview image is simulated in the Viewfinder Lite software by intensifying the brightness level The Viewfinder Lite preview image may appear of substantially lower quality than the final captured image at exposure time settings slower than 1 4 second Zoom Button The Zoom button provides a live Viewfinder Lite preview which is zoomed on the center 25 of the camera image area This portion of the image is displayed at the full CCD resolution 640x480 portion of 1392x1040 providing the highest level of detail of all of the Viewfinder Lite modes The effect of the Sharpness setting located under the Options menu is previewed only in the Zoom mode and may assist in the focusing process The frame rate of the preview image is slower than Fast mode same as Full mode and is limited by the exposure time setting Full Button The Full button provides a live Viewfinder Lite preview of the entire camera image area by scaling the CCD resolution image into the 640x480 pixel preview area providing increased resolution over Fast mode but de
114. tails Moving the marker triangles produces an immediate effect on the preview image and likewise the histogram is continuously updated in real time as the camera s scene changes Reset Button The Reset button resets the level adjustments which were previously set by the user Update Button Windows Only The Update button displays the histogram for the current frame of the Viewfinder preview Freeze Checkbox Macintosh Only Level adjustments are automatically applied in real time to the preview image histogram so there is no need for an Update button on the Macintosh To freeze the histogram however so that it does not change due to noise to changes in the scene viewed by the camera or to adjustments of the markers click the Freeze checkbox To unfreeze the histogram click again User Preset Tab The User Preset tab Figure 9 allows you to save current Viewfinder Lite capture settings or load previously saved settings All user selected settings are saved including white balance mode frame accumulation options sensitivity exposure mode spot size capture resolution and exposure time Instruction Manual 81 Viewfinder Options Menu cont Lite Cota alero Level Achaia Lise Pare Loss para Save Button Load Button Current Setting Figure 9 Setting Dialog Box User Preset Tab Mr der hetimiga uar berg 7 evel deiantrereg NN Bem Berii imen Fie E mima na EA Zen
115. the Restore option Method B Use the following keyboard shortcut F8 To display or hide the Studio Lite Toolbar Method A Instruction Manual Studio Lite 135 Studio Lite 136 Working Within the Studio Lite Application Window cont 1 Click the View menu 2 To display the Toolbar place a checkmark next to the Toolbar command To hide the Toolbar remove the checkmark Method B Use the following keyboard shortcut F3 This will toggle between displaying and hiding the Toolbar To display or hide the Studio Lite Status Bar Method A 1 Click the View menu 2 To display the Status Bar place a checkmark next to the Status Bar command To hide the Status Bar remove the checkmark Method B Use the following keyboard shortcut F4 This will toggle between displaying and hiding the Status Bar To display or hide the Studio Lite Toolbox Method A 1 Click the View menu 2 To display the Toolbox place a checkmark next to the Toolbox command To hide the Toolbox remove the checkmark Method B Use the following keyboard shortcut F5 This will toggle between displaying and hiding the Toolbox Application Suite Measuring Color Values Studio Lite To measure color values within an image You may position your mouse pointer over any portion of the active image window To view the color values under each channel red green and blue for a particular pixel refer to the Color Channel Valu
116. three common tools pertaining to image viewing editing and drawing These comprise Marquee Zoom and Pan The Toolbox can be repositioned to any place on the Desktop with the mouse Windows only Double clicking on the upper border of the Toolbox will return it to the Studio Lite application window Marquee Tool 2 Zoom Tool Pan Tool 1 Figure 20 Toolbox E ber a K Figure20a Toolbox Macintosh Marquee Tool The Marquee tool allows you to select any rectangular portion of the image in the active image window as the region of interest To select an area click and hold the mouse button Windows left button on a starting point drag the mouse pointer to highlight the desired area and release the mouse button All subsequent operations will affect only the selected area Zoom Tool The Zoom tool allows you to magnify and reduce the view of the image in the active image window The image window s title bar displays the magnification or reduction percentage at all times Clicking on the image increases the magnification in increments of 100 Holding down the Shift key while clicking on the image decreases the magnification Hold down the Control key while clicking on the image to restore the image to its actual size 100 Pan Tool The Pan tool allows you to move an image within the active image Application Suite Toolbox cont Studio Lite window performing the equivalent function
117. tically updated to provide optimum color reproduction This feature should be selected when viewing images which have discernible regions of true black The setting is reflected in both the preview as well as the captured image and remains in effect until a different mode of color balancing is selected the Focus Finder is activated or the Move Instruction Manual 89 Viewfinder Lite 90 Capture Toolbar cont Spot button is pressed to on One Push White Balance Button The One Push White Balance button calculates the white balance based on the entire camera scene allowing the user to calibrate the Red Green and Blue RGB color correction coefficients by pointing the camera at a white target or light source The calibration is performed immediately upon selecting this option The coefficients stay fixed for all subsequent captures until a different mode of color balancing is selected the Focus Finder is activated or the Move Spot button is pressed to on This setting allows accurate adjustments for applications in which the scenes remain relatively static and unchanged These settings are reflected in both the preview as well as the captured image Move Spot Button The Move Spot button allows you to reposition the spot meter under automatic exposure mode When this button is depressed you may place the spot meter on the region of the subject under which you would like the auto exposure calculated By default the
118. time is calculated based on the image data present within the region defined by the spot meter This control is enabled only under automatic exposure AE mode and is disabled under the AE Lock and manual exposure modes Busy LED The Busy LED indicates the status of the capture process When the LED is on the camera is either capturing an image or processing image data and new commands or camera functions are not accepted When the LED is off the camera is idle and awaiting user input Installing the Hardware 2 Installing the Software on Installing the Windows Software on Windows Installing the Device Drivers Windows 95 98 PCI Device Driver Installation 1 Before you turn your computer on be sure the PCI interface card is properly installed and that your camera is connected 2 Turn your computer on As Windows loads your computer will display a New Hardware Found dialog box and detect the PCI interface card as PCI Multimedia Device Press the Next button to continue with the installation 3 At the following screen choose the bottom option and press Next if de pra rl dic E che IT ac aa ie ire eg Be Get pap eran reg me Eiere P Dada bid al ha dian ira ci Ka Mrs om pia om cb Be lr pa me cma 4 Choose Other devices from the list of devices and press Next Al be loe dl Cea des praa cies ll Epi s conde Liers La sud Lol e ls E mici do il gar o ben Ema Si
119. ting the image in the active image window with any accessible printer 1 Prinder Epi o ai Berian Pr ale Si arial zj Lope fi O Daai Fagat de iii y Fumar Sei Ir Paper fource 80 pages from Las IS Ad pra aen AER eg diia Covet Frei H Oe A BA Figure 5b Print Dialog Box Macintosh 154 Application Suite File Menu cont Print Preview Command The Print Preview command brings up the Print Preview dialog box Figure 6 which allows you to inspect the printed layout of the active image on your screen COT AA bai Figure 6 Print Preview Dialog Box Instruction Manual Studio Lite 155 Studio Lite File Menu cont ja Pra priae E Figure 6a Print Preview Window Macintosh Print Setup Page Setup Command The Print Setup command Windows or Page Setup command Macintosh brings up the setup dialog box Figure 7 amp 7a which allow you to specify printer properties such as paper size and orientation 156 Studio Lite File Menu cont Pem ei HEI been nee Se c Gas Park Tapa HP La de ie MTI Ps Tapan Vase SAA Las Blies E pw E ena a pit F paca Figure 7 Print Setup Dialog Box Lorettrzier A Page rta Fage Ali CH fara tin ein Paper lebier imami Si Migrar Iw EI Scala 16m 1 Ca a Figure 7a Page Setup Dialog Box Macintosh Recently Opened Images Windows Only Rec
120. ttings are reflected in both the preview as well as the captured image AE Lock Command Working in conjunction with the Viewfinder Lite s automatic exposure AE mode the AE Lock command switches between the activation and deactivation of the auto exposure lock feature of the Viewfinder Lite application When selected the AE Lock button in the Capture toolbar is automatically activated locking the exposure time and gain at their current settings and temporarily disabling the auto exposure function When this command is not selected the automatic exposure feature is re enabled Exit Command Windows Only The Exit command closes the Viewfinder Lite application window and quits the program Instruction Manual 73 Viewfinder Lite 74 Options Menu The Options menu Figure 6 or 6a allows you to select the toolbars to be displayed within the Viewfinder Lite application window This menu also includes selections found in the Options toolbar with the following seven commands Grayscale Settings Rotate 180 Mirror Flip 16 Bits Channel and Sharpness E an iter Ger e Capi Manta x a e Jar Toa Sei e Foca onion Capture Toolbar Option Options Toolbar Option Focus Toolbar Option e D n Dm Status Bar Option Exposure Toolbar Option e Las sta Grayscale Command Lem Settings Command Rotate 180 Command Mirror Command Flip Command 16 Bits Channel Opti
121. u Macintosh New Command The New command Windows Ctrl N Macintosh Cmd N creates a new blank image window The size of the window is Application Suite File Menu cont Studio Lite determined by the dimensions of any image data which may exist in the Windows Clipboard By default the window size is 320x240 pixels Open Command The Open command Windows Ctrl O Macintosh Cmd 0 allows you to open any supported image file into a new image window Close Command The Close command Windows Ctrl C Macintosh Cmd W closes the active current image window Close All Command Macintosh only for Windows see Close All in the Window menu The Close All command Opt Cmd W closes all the still image windows Save Command The Save command Windows Ctrl S Macintosh Cmd S saves the image in the active image window to disk Save As Command The Save As command allows you to save the image in the active image window to a destination file and format other than ts original source Revert Command The Revert command Windows Ctrl R Macintosh Cmd R discards all changes made to the image in the active image window since the last save operation and reloads the source file Instruction Manual 153 Studio Lite File Menu cont Print Command The Print command Windows Ctrl P Macintosh Cmd P brings up the Print dialog box which allows you to select options for prin
122. ual Viewfinder Lite 41 Viewfinder Lite 42 Working Within the Viewfinder Lite Application Window cont To display or hide the Exposure toolbar 1 Click the Options menu 2 To display the Exposure toolbar place a checkmark next to the Exposure Toolbar option To hide the Exposure toolbar remove the checkmark For Macintosh users The various buttons and menus are fixed on the Macintosh Viewfinder window and not separated into different toolbars The Macintosh version does not have a Status Bar Viewfinder Lite Windows Studio Lite Macintosh Manipulating Images The Viewfinder Lite application offers a variety of controls for manipulating the orientation and display of the preview and captured images To capture images in grayscale Method A 1 Click on the Options menu 2 Select the Grayscale command Method B Click the Toggle Grayscale button located in the Options toolbar To rotate the image Method A 1 Click on the Options menu 2 Select the Rotate 180 command Method B Click the Rotate 180 button located in the Options toolbar To flip the image horizontally along the vertical axis Method A 1 Click on the Options menu 2 Select the Mirror command Instruction Manual Viewfinder Lite 43 Viewfinder Lite 44 Manipulating Images cont Method B Click the Mirror button located in the Options toolbar To flip the image ve
123. udio Lite Help Command The Studio Lite Help command displays online help in a new window Amani Aria Hai Hem Dales adie ile Frot Figure 18a Help Menu Macintosh The window displays in a Browser window the same information as this printed manual and can be searched for specific topics or keywords Instruction Manual 167 Studio Lite 168 Toolbar The Toolbar Figure 19 and 19a provides access to functions governing all aspects of the Studio Lite application including file management and image editing It is composed of the following eleven buttons New Open Save Cut Copy Paste Undo Redo Viewfinder Lite Full Screen and Print Windows Only The Toolbar can be manually repositioned to any place on the Windows desktop using mouse drag and drop Double clicking on the upper border of the Toolbar will return it to the application window Paste Button Cut Button Redo Button Save Button Full Screen Button New Button Print Button j ja ajeje a Open Button Viewfinder Lite Button Copy Button Undo Button Figure 19 Toolbar Hase Denko 3 Coy le el re el D E i kihat ps EG Figure 19a Toolbar Macintosh New Button The New button creates a new blank image window The size of the window is determined by the dimensions of any image data which may exist in the clipboard By default the window size is 320x240 pixels Open Button
124. ug in Installation Instruction Manual 25 Installing the 3 Installing the Software on Software on Macintosh cont Macintosh 10 After the installation of the Studio Lite software and driver is completed you will be prompted to restart your computer Click Restart to apply all changes to your system Winds Dalai pias piikin Leste MET ir que ere Sindee rs Conlon ta perio Moriles da Figure A 10 Installation Completed 11 When you have restarted your computer system the Studio Lite files will be located in the Digital Microscopy Camera folder on your desktop default location unless you specified a different location during the installation process 10 ibana 132 68 available K Studio Lite Stadio Lite Guide E Viewlinder Setting oor subis arra A Corts dat Gamma giak Figure A 11 The Studio Lite Files 26 Installing the Software on Macintosh 4 Capturing Procedure Capturing Procedure Capturing Procedure 1 Perform all necessary optical adjustments on the microscope 2 Insert an LBD color temperature conversion filter in the light path if a bright field is observed Then adjust the light voltage to the photograph reference voltage Although the camera will perform the WB adjustment better quality images will be provided Start Viewfider Lite application Switch the light path of the microscope to the camera light path E Push Preview Start Stop button 1 in Fig 1 Set the
125. ugh 7 above The preview image is updated to reflect the color balance settings To disable all white balance operations Method A 1 Click on the Options menu 2 Select the Settings command This will bring up the Settings property sheet or dialog box 3 Click the Color Balance tab Instruction Manual Viewfinder Lite 49 Viewfinder Adjusting the Color Balance cont Lite 4 Select Off 5 Click OK to close the property sheet and apply the changes Method B 1 Click on the Settings button located in the Options toolbar This will bring up the Settings property sheet 2 Repeat steps 3 through 5 above Method C 1 Click on the Viewfinder menu 2 Select the White Balance command and select Off 50 Viewfinder Lite Windows Studio Lite Macintosh Adjusting Levels Viewfinder Lite The Viewfinder Lite application allows you to perform dynamic adjustments to the histogram of the preview displayed in the Viewfinder Lite image window To adjust the shadow value Method A 1 Click on the Options menu 2 Select the Settings command This will bring up the Settings property sheet or dialog 3 Click the Level Adjustment tab 4 Click on the Channel pull down list or popup menu 5 Select the color channel upon which you would like the adjustment performed You may choose from the composite RGB channel or the individual color channels Red Green and Blue 6 Windows
126. vels cont 3 Click the User Preset tab 4 Press the Save button This will bring upt he Save Settings dialog box 5 Enter a filename and select the location to which you would like the settings saved 6 Click the Save button Method B 1 Click on the Settings button located in the Options toolbar This will bring up the Settings property sheet or dialog box 2 Repeat steps 3 trhough 6 above To load previously saved User Preset settings Method A 1 Click on the Options menu 2 Select the Settings command This will bring up the Settings property sheet or dialog 3 Click the User Preset tab 4 Press the Load button This will bring up an Open dialog box 5 Select the desired settings file 6 Make your choice and click the Open button Method B 1 Click on the Settings button located in the Options toolbar This will bring up the Settings property sheet or dialog box 2 Repeat steps 3 through 6 above Instruction Manual Viewfinder Lite 55 Viewfinder Lite 56 Using Automatic Exposure The Viewfinder Lite application supports fully automatic exposure AE control In this mode the Viewfinder Lite calculates the appropriate exposure for the current scene based on the region specified by the spot meter To select the automatic exposure mode 1 Click the Exposure Mode pull down list or popup menu located in the Exposure toolbar 2 Select Aut
127. wfinder Lite application window to its previous state Move Command The Move command allows for the manual positioning of the Viewfinder Lite application window within the Windows desktop using the keyboard s directional arrow keys Size Command The Size command allows for the manual sizing of the Viewfinder Lite application window using the keyboard s directional arrow keys Minimize Command The Minimize command reduces the Viewfinder Lite Pro application window to an icon in the Windows taskbar Maximize Command The Maximize command is not available within the Viewfinder Lite application Instruction Manual 67 Viewfinder Control Menu cont Lite Close Command The Close command closes the Viewfinder Lite application window and quits the program 68 Viewfinder Lite Windows Studio Lite Macintosh Menu Bar Viewfinder Lite For Windows The strip below the Title Bar is called the Menu Bar Figure 4 Control over the range of camera control tools offered by Viewfinder Lite is available through the three menu selections Viewfinder Options and Help Viewfinder Menu Help Menu etc pics Hee Options Menu Figure 4 Menu Bar For Macintosh The strip across the top of any window is called the Title Bar Figure 2a The left hand box is the Close Box which allows you to close the window without quitting the application To quit the program use the File menu in the Menu Bar
128. with the Marquee tool click on the Selection radio button 8 Select the number of copies you would like printed of the current image To print multiple copies in a sorted fashion click on the Collate checkbox 9 Click OK to print the image Method B 1 Click once on the desired image to make it the active image window Windows only If you would like to print only a portion of the selected image use the Marquee tool to select the desired region of interest 2 Windows Use the following keyboard shortcut Ctrl P Macintosh Use the following keyboard equivalent Cmd P This will bring up the standard Windows or Macintosh Print dialog box Instruction Manual 115 Studio Lite 116 Printing an Image cont 3 Repeat steps 4 to 9 above Method C 1 Click once on the desired image to make it the active image window Windows only If you would like to print only a portion of the selected image use the Marquee tool to select the desired region of interest 2 Click the Print button located in the Studio Lite Toolbar This will bring up the standard Windows Print dialog box 3 Repeat steps 4 to 9 above Method D 1 Click once on the desired image to make it the active image window Windows only If you would like to print only a portion of the selected image use the Marquee tool to select the desired region of interest 2 Click on the File menu 3 Select the Print Preview command A repre
129. with the digital camera system To run the Studio Lite application 1 Click on the Start button located on the Windows taskbar 2 Point to Programs The Programs menu will appear 3 Click on Studio Lite The Studio Lite application window will appear Macintosh On the Macintosh platform the Viewfinder and Studio Lite functionality is integrated into the single Studio Lite application Therefore the Mac user can ignore references to Studio being a separate application program the Viewfinder application having to be started up separately etc On the Macintosh when the user captures an image using the Viewfinder the captured image immediately opens in a new window of the same application This chapter contains a number of Windows only explanations which the Mac user can ignore But functionality described in this Studio Lite chapter that is not explicitly marked as Windows only is available to users of both platforms for still image or Studio windows Keep in mind that any open window in Studio Lite which is not the Viewfinder window the preview window is here called a Studio window Studio Lite Creating a New Image The New command lets you create a blank untitled Studio Lite image You can use this command to create a new image with the exact same pixel dimensions as an image or selection that has been copied to the Clipboard To create a new image Method A 1 Click on the File menu 2 Select the New c
130. ying a Region of Interest Studio Lite The region of interest ROI specifies the portion of the active image upon which a number of Studio Lite operations will be applied including image editing adjustment and filtering tools To specify a specific region of interest 1 Click once on the desired image to make it the active image window 2 Click on the Marquee button located in the Studio Lite Toolbox When placed over the active image the mouse pointer will be designated by a crosshair 3 Click and hold the mouse button Windows left hand button at the starting point of the selection 4 Drag the mouse to encompass the desired region of interest 5 Release the mouse button The specified region of interest will be bordered by a rectangular frame To select the entire active image 1 Click once on the desired image to make it the active image window 2 Click the Edit menu 3 Choose the Select All command The entire active image will be specified as the region of interest and bordered by a rectangular frame To remove a previously specified region of interest Instruction Manual 127 Studio Lite 128 Specifying a Region of Interest cont Method A 1 Click once on the desired image to make it the active image window 2 Click the Edit menu 3 Choose the Deselect command Method B Click once using the mouse button Windows left hand button anywhere within the active image window out

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