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MINDRAY BC-2800 Hematology Analyzer Operaters

1. Other Auto Report title Recorder Printer Reagents for gt 99 Counts MENU gt Move cursor 1 Select setting item F1 Select setting group Figure 7 17 Auto transmission Press T or J to move the cursor to On or Off to enable or disable the function and press ENTER to confirm the selection When you are done enabling or disabling the auto transmission press T or J to move the cursor to other printing settings you want to change 7 2 2 3 Changing report titles external keyboard needed You can set the tiles of the reports to be printed out by the recorder or external printer Follow the steps below to do so 1 Atthe Setup screen press F1 to select the Print amp comm group 2 Press T or 1 to move the cursor to the edit box right of Recorder or Printer in the Report title field 3 Press gt or to move the cursor within the edit box Use an external keyboard to enter a letter or character at the position where the cursor is located Press DEL if you want to delete the character before the cursor Press Backspace on the external keyboard if you want to delete the character after the cursor 4 When you are done entering the report tile press T or 4 to move the cursor to other settings you want to change 7 2 2 4 Exiting When you are done changing al
2. Reagents for gt 99 Counts MENU 7 Move cursor f Select setting item F1 Select setting group Figure 7 19 Setting date and time Setting date Follow the steps given below to set the system date Press PgUp or PgDn to enter the desired date At the Setup screen press F1 to select the Date amp Time group Press T or 4 to move the cursor to the edit box right of Year Month or Day in the 7 14 Auto Hematology Analyzer Operation Manual V1 0 Setup When you are done entering the date press T or J to move the cursor to other settings you want to change 7 2 3 2 Setting date format You may choose one of the three date formats YYYY MM DD MM DD YYYY or DD MM YYYY Follow the steps given below to do so At the Setup screen press F1 to select the Date amp Time group Press fT or J to move the cursor to the combo box right of Format in the Date field Press ENTER to display the pull down menu as Figure 7 20 shows Setup Ready 23 43 Settings Reagent Time Print amp comm Date amp time Gain Count Ref range Other Hour Minute gt lt gt lt gt a gt lt gt lt TD gt Second Reagents for gt 99 Counts MENU lt 7 Move cursor T Select setting item F1 Select setting gro
3. Reagents for gt 99 Counts MENU T 1 lt 7 Select sub menu ENTER Enter sub menu Figure 8 19 Entering self test screen Press ENTER to enter the Self test screen as Figure 8 20 shows If you want acquire help information press HELP Service est Ready 00 33 Tubing Result O Machine O Valve O Circuit WBC s RBC s Count Time Aperture v Vacuum Pressure Filter Reagents for gt 99 Counts MENU F1 Select test group 1 Select test ENTER Execute Figure 8 20 Self test screen The available self test items are divided into four groups the Tubing Machine Valve and 8 18 Auto Hematology Analyzer Operation Manual V1 00 Maintenance Circuit as shown on the left of the screen You can switch between the groups by pressing F1 and once a group is selected the test items including their test results if available belonging to that group will be displayed on the right of the screen You may press PRINT to print out the displayed results Follow the instructions given below to conduct every test and if you want to acquire help information press HELP 8 3 1 Testing Tubing At the self test screen press F1 to select the Tubing group as Figure 8 20 shows The following test items will be displayed on the right of the screen 1 Count Time WBC S It measures the duration of a WBC count namely how many seconds it take
4. Review Quality control Self test 4 7 Log Calibration Config Shutdown Print D 10 16 20 26 fL Figure 8 17 Entering status screen Press ENTER to enter the Status screen as Figure 8 18 shows Service Ready 00 32 Item Range Envir Temp C 15 0 35 0 HGB zerao v 0 00 0 20 HGB blank 3 40 4 80 Vacuum 175 205 Reagents for gt 99 Counts MENU Figure 8 18 Status screen 8 16 Auto Hematology Analyzer Operation Manual V1 0 Maintenance Note that you can only view the displayed status items without changing them If any of the displayed item exceeds the given range see Chapter 9 Troubleshooting for solutions Auto Hematology Analyzer Operation Manual V1 0 8 17 Maintenance 8 4 System Self Test The system self test is a major way to locate system errors Follow the instructions given below to view and check the available self tests items Press MENU to enter the system menu and press the appropriate arrow keys T 4 gt to move the cursor to Service gt Self test as Figure 8 19 shows Service Item Range Envir Temp C 26 0 HGB zerao v 0 01 HGB blank v 4 51 Vacuum Count Review Maintenance Quality control gt Status Setup 15 0 35 0 0 00 0 20 3 40 4 80 175 206 Calibration Shutdown Print
5. Transmit Baud 9600 Parity None Handshake No Auto Off v 21 20 Reagents for gt 99 Counts MENU 7 Move cursor f Select setting item F1 Select setting group Figure 7 11 Selecting printing format Press T or J to select Format 1 Format2 Format3 or Format4 Press ENTER to confirm the selection See Table 7 2 for the definition of the four formats Table 7 2 Printing Formats Printing i Format1 Format2 Format3 Format4 Device Recorder Parameters Parameters Parameters Parameters histograms histograms Printer Whole page Whole page Whole page Whole page with histograms without with histograms without histograms histograms 3 Auto Printing The auto printing function refers to the analyzer s ability to automatically print out the analysis results once it is done Follow the steps given below to enable or disable this function Atthe Setup screen press F1 to select the Print amp comm group Press T or J to move the cursor to the combo box right of Auto in the Print field Press ENTER to display the pull down menu as Figure 7 13 shows Auto Hematology Analyzer Operation Manual V1 0 7 9 Setup Setup Ready 21 20 Settings Reagent Print Transmit Print amp comm Date amp time D
6. the decimal point The valid usable container volume is OL 99 9L Make sure your input is within this range 4 When you are done entering the volume press T or to the next setting to be changed 7 2 1 3 Entering expiration dates of reagents You can specify the expiration dates for the diluent rinse and lyse Once any of these reagents is expired the system will alert the user to replace that reagent Follow the steps given below to enter the expiration dates 1 Atthe Setup screen press F1 to select the Reagent group 2 Press T or J to move the cursor to the edit box right of the Diluent Rinse or Lyse in the Expt Date area 3 Press PgUp or PgDn to enter the desired expiration date in the YYYY MM DD format Note that the service life of the reagent after being opened is 60 days The expiration date to be entered should be the open container date 60 days or the expiration date marked on the packing of the reagent whichever is smaller For exanpl es 1 If the rinse container is opened on April 3rd 2004 and the expiration date marked on the packing is December 15th you should enter June 3rd 2004 as the valid expiration date for the rinse 7 6 Auto Hematology Analyzer Operation Manual V1 0 Setup 2 If the rinse container is opened on April 3rd 2004 and the expiration date marked on the packing is May 5th you should enter May 5th 2004 as the valid expiration dat
7. 300 0 999 1 12 Auto Hematology Analyzer Operation Manual V1 0 Introduction Parameter Condition Reproducibility CV WBC x10 L 4 0 7 5 lt 3 0 7 6 15 0 lt 2 0 RBC x10 L 3 00 4 50 lt 2 0 4 51 6 50 lt 15 HGB g L 110 180 lt 15 MCV fL 70 0 100 0 lt 1 0 PLT x10 L 100 200 lt 5 0 201 500 lt 4 0 1 5 4 Display Color LCD 640x480 1 5 5 Input Output Two RS232 serial ports one for computer and the other for scanner One parallel interface for printer or floppy disk drive One PS 2 keyboard interface the keyboard is optional A power interface for the floppy disk drive only works with Mindray s special power cable 1 5 6 Scanner optional TYSSO CCD 82 or its compatible 1 5 7 Built in Thermal Recorder 1 5 8 Printer optional EPSON LQ 300K LQ 300K or EPSON LQ 1600K or their compatible 1 5 9 Alarms See Chapter 9 Troubleshooting for the error codes 1 5 10 Reagents Diluent M 30D DILUENT Rinse M 30R RINSE Lyse M 30L LYSE E Z cleanser Enzyme cleanser M 30E CLEANSER Probe cleanser M 30P CLEANSER Auto Hematology Analyzer Operation Manual V1 0 1 13 Introduction 1 5 11 Power Voltage AC 220V415 50 60Hz 1Hz or AC 110V 15 50 60Hz 1Hz Consumption 180 VA Fuse 250 V T2A or 125V T4A 1 5 12 Ambient Temperature and Humidity Ambient Temperature 15 C 30 C When overheated the system will
8. Gain Any key to mute Enabled Count Ref range Other Alarm time s io LCD contrast 204 0000000 Reagents for gt 99 Counts MENU 7 Move cursor f Select setting item F1 Select setting group Figure 7 12 Setting alarm time Press PgUp or PgDn to adjust the time When you are done adjusting the time press T or to move the cursor to other settings you want edit 7 2 7 4 Setting PBM color administrator authority needed Follow the steps below to set the background color of the Count screen when it is in the prediluted mode Obtain the administrator authority as instructed in Chapter7 1 1 Atthe Setup screen press F1 to select the Other group Press T or J to move the cursor to PBM color as Figure 7 39 shows Setup Ready 23 56 O Reagent O Print amp comm O Date amp time O Gain Any key to mute Enabled gt Sea LCD contrast 204 Other Alarm time s 10 PMB color Black Note PMB means EENS ackground Blue Reagents for gt 99 Counts MENU lt 7 Move cursor T Select setting item F1 Select setting group Figure 7 39 Setting PBM color 7 28 Auto Hematology Analyzer Operation Manual V1 00 Setup Press tT or J to select Black or Blue and press ENTER to confirm the selection Press T or J to move the cu
9. None Handshake Auto Report title Recorder Printer Reagents for gt 99 Counts MENU lt 7 Move cursor T Select setting item F1 Select setting group Figure 7 16 Setting handshake Press T or 1 to move the cursor to On or Off to enable or disable the handshake and press ENTER to confirm the selection When you are done enabling or disabling the handshake press T or J to move the cursor to other printing settings you want to change gt A Auto transmission If the auto transmission is on this analyzer will automatically transmit the analysis result to an external computer when the sample analysis is done If this function is off you have to transmit the analysis results manually Follow the steps given below to enable or disable the auto transmission Atthe Setup screen press F1 to select the Print amp comm group Press T or J to move the cursor to the combo box right of Auto in the Transmit field Press ENTER to display the pull down menu as Figure 7 17shows 7 12 Auto Hematology Analyzer Operation Manual V1 0 Setup Setup Ready 23 42 Settings Reagent Print Transmit Print amp comm Date amp time Device Recorder Baud 9600 Gain Format Format 1 Parity None Count Ref range Auto Off Handshake
10. Auto Hematology Analyzer Operation Manual V1 00 1 17 Introduction 1 6 2 Display Areas Title Count Mode System System Status time Window Error Messages Reagent Status MENU On line Help Figure 1 8 Display areas Title area Displays the screen title such as Count or Review Count Mode area System Status area Whole Blood All Parameter Prediluted All Parameter Whole Blood WBC HGB Prediluted WBC HGB Whole Blood RBC PLT Prediluted RBC PLT Displays current count mode Six count modes are available Displays whether the system is ready for sample analysis Three modes are available Ready Running and Waiting System Time area Displays system Window area Displays all types of operation information Error Messages Area Displays error messages Reagent Status On Line Help details Displays help information Displays status of the remaining reagents See chapters 3 and 7 for Auto Hematology Analyzer Operation Manual V1 0 1 6 3 Input Devices Introduction The input devices includes a START key 18 key keypad and PS 2 keyboard optional Note If your analyzer is not equipped with a PS 2 keyboard you will not be able to use the functions controlled by the keyboard only The START key is located behind the sample probe The keypad consis
11. At the Self test screen press F1 to select the Circuit group as Figure 8 23 shows You can test the A D interrupt at this screen by pressing ENTER to see whether the WBC RBC and PLT signals can be properly converted into digital signals Service Ready 00 34 O Tubing O Machine O Valve Circuit A D interrupt Reagents for gt 99 Counts MENU F1 Select test group f Select test ENTER Execute Figure 8 23 Testing A D converter Auto Hematology Analyzer Operation Manual V1 0 g 8 21 Maintenance 8 5 Log The log records all the major events taking place during the running of this analyzer It helps the service engineers diagnose system errors Press MENU to enter the system menu and press the appropriate arrow keys T to move the cursor to Service Log as Figure 8 24 shows Service O Machine O Valve Circuit Count Review Quality control Setup Calibration Shutdown A D interrupt gt Status Vv Self test Vv Config Print Maintenance Reagents for gt 99 Counts MENU T 1 lt 7 Select sub menu ENTER Enter sub menu Figure 8 24 Entering log Press ENTER to enter the Log screen as Figure 8 25 shows Service Ready 00 34 All Time Ype Times Information O Settings Error O Oth
12. expiration date Controls WBC File No 1 a Lot N Ss File No 1 Lot No 000000 Exp Date 01 27 2004 p D fL Para Mean Range Para Mean Range Total WBC Gran 0 Mode RBC Gran Time HGB HCT PLT MCV fL Made Lymph MCH Lymph MCHC PDW c PCT Oo 6 10 15 20 25 fL Figure 4 4 QC Edit Screen 1 File Number The file number of the selected QC file will be displayed in the File No field where you can only view the number without altering it 2 Entering lot number You should enter the lot number of the control material to be used for the upcoming QC analysis in the edit box right of Lot No Follow the steps given below to do so A Press ft or to move the cursor to the edit box as Figure 4 5 shows Contrals WBC File No 1 el File No 1 Lot No 007645 Exp Date 01 27 2004 si D fL Para Mean Range Para Mean Range Total WBC Gran 0 Mode RBC Gran Time HGB HCT Zz PLT MCV iE Mode Lymph MCH Lymph MCHC PDW r PCT 0 5 10 16 20 25 fL Figure 4 5 Entering Lot No 4 4 Auto Hematology Analyzer Operation Manual V1 0 Quality Control Press or gt to move the cursor to the desired position within the box Press PgUp or PgDn or the numeric keys of a keyboard to enter a digit at the position where the cursor is located D To modify a digit at a certain position
13. V1 0 Installation Chapter 2 Installation 2 1 Unpacking When you receive this analyzer carefully unpack and check it File a claim immediately with the shipping carrier if you find any physical damage Compare the delivered goods against the packing list to ensure that the shipment is complete If the shipment appears incomplete notify Mindray Customer Service Department or your distributor immediately Auto Hematology Analyzer Operation Manual V1 00 2 1 Installation 2 2 Installation Requirements 2 2 1 Installation Environment The environment should be as free as possible from dust mechanical vibrations loud noises and electrical interference Avoid proximity to brush type motors flickering fluorescent lights and electrical contacts that regularly turn on and off Avoid placing this analyzer in direct sun or in front of a source of heat or draft Operating this analyzer in ways other than those specified by this manual may damage it and leads to wrong analysis result 2 2 2 Space Requirement Be sure to place this analyzer on a table with enough space for the reagent containers Note Be sure to keep the reagent at the same height as the analyzer 2 2 3 Power Requirements This analyzer requires a properly grounded socket supplying AC 220V 15 or 110V 15 The power frequency should be 50 60 1Hz the maximum power consumption 180VA fuse 250V 2A or 125V 4A If possible connect this analyzer to a dedica
14. re the entered number is invalid as Figure5 6 Calibration Manual Ready 15 49 Whole Blood O Prediluted Para Factor WBC 132 0 Invalid input Reagents for gt 99 Counts MENU Figure5 6 The dialog box to remind you of invalid values 5 6 Auto Hematology Analyzer Operation Manual V1 0 Calibration Press ENTER and re enter the factors If the changed factors are all within the calibration range a dialog box will pop up to remind you to save the new factors as Figure 5 7 shows Calibration Ready 15 49 Whole Blood Para Factor Time O Prediluted 102 0 01 27 2004 aca Save new factors 1 25 2004 EE 200 PLT TOU 01 27 2004 Reagents for gt 99 Counts MENU Figure 5 7 The dialog box to remind you to save the changes To save the new factors press or gt to move the cursor to Yes and press ENTER to return to the Manual calibration screen with new factors 5 Other operations At the Manual screen if you want to print out the calibration factors press PRINT if you want to acquire help information press HELP If you want to return to the system menu press MENU 5 3 4 Testing New Factors After the new factors are saved go to the Count screen to analyze the calibrator or control material for at least f
15. Loose tubing connection 3 Inappropriate WBC count time setting Solution 1 Check if the diluent or rinse is sufficient If not change a new container of diluent of rinse as instructed by Chapter 2 3 2 Check the tubing connections If necessary reconnect the tubing as instruction by Chapter 2 3 3 Ifthe error still remains access Setup gt Password to gain the administrator authority as instructed by Chapter 7 1 1 and then access Setup gt Settings gt Count and adjust the WBC count time as instructed by Chapter 7 2 5 2 4 If the error still remains turn off this analyzer and call Mindray Customer Service Department or the distributor 9 2 9 Background Abnormal At least one parameter failed the background check Solution At the Count screen press F3 to do the startup procedure If the error still remains access Service gt Maintenance and do the Probe cleanser cleaning procedure as instructed When the cleaning is done return to the Count screen and check the background again to seen whether the error is cleared If not call Mindray Customer Service Department or the distributor 9 2 10 Rinse Expired Possible causes _ rinse has expired or its expiration date is not correctly set Solution Check the rinse If it is expired change a new container of rinse as instructed by Chapter 2 3 if not access Setup gt Settings gt Reagents and adjust the expiration date as instructed by Chapter 7 2 1
16. TAE ca an ID 118 Time 01 24 2004 F2 Edit upper limit 1 Select parameter Reagents for gt 99 Caunts MENU F1 Select the desired function Figure 6 23 Trend screen 2 At either screen you can press lt or gt to view the results displayed below the parameter box of every point presented in the graph The current cursor position is displayed right of No and the time at which the sample was analyzed is displayed right of Time You can also press PgUp or PgDn to jump forward or backward by 20 samples Auto Hematology Analyzer Operation Manual V1 0 6 17 Sample Review The trend is interpreted as follows 1 The x coordinate represents how many sample results have been selected The y coordinate represents the analysis results of the displayed parameters 2 For every parameter the upper dash line of its trend represents the upper limit of the expected range 10 above the mean of the analysis result In case of Figure 6 22 the upper limit is 10 2 3 For every parameter the lower dash line of its trend represents the lower limit of the expected range 10 below the mean of the analysis result In case of Figure 6 22 the lower limit is 8 4 4 For every parameter its mean is displayed between the values of the upper dash line and of the lower dash line In case of Figure 6 22 the mean is 9 3 5 For every parameter the three numbers on the right of it
17. press PgUp or PgDn or the numeric keys of a keyboard to enter the digit at the position where the cursor is located To enter the end location 00005 press T or J to move the cursor to the edit box right of To press or gt to move the cursor within the box press PgUp or PgDn or the numeric keys of a keyboard to enter the digit at the position where the cursor is located Press T or to move the cursor to Select or de select as Figure 6 9 or Figure 6 10 shows Auto Hematology Analyzer Operation Manual V1 0 6 7 Sample Review Review Ready 20 42 Location ct From 00001 De Select To 00005 Exit Reagents for gt 99 Counts MENU Figure 6 9 Select 5 samples Review Ready 20 45 Location From 00001 To 00006 Reagents for gt 99 Counts MENU Figure 6 10 De select 5 samples Press ENTER to confirm the selection or de selection Press T or J to move the cursor to Exit and press ENTER to return to the Review screen to see the selected or de selected results as Figure 6 10 or Figure 6 12 shows 6 8 Auto Hematology Analyzer Operation Manual V1 0 Sample Review Review Ready 20 44 Reagents for gt 99 Caunts MENU Searched Hi
18. to enter the system menu Press the appropriate arrow keys T J 1 J to nove the cursor to Setup Settings as Figure 7 5 shows Whole Blood Alll Current sample WBC 8 1 x 10 L Lymph 3 7 x 10 L Mi d 0 7 x1 W ID 118 Gran 4 7 x 10 L p Time 01 24 2004 13 55 Lymph H 41 0 Mid 8 0 Mode WB All 0 100 200 300fL e Gran 51 0 HGB 24 g L Name RBC 4 08 x 10 2 L HCT L 36 0 Count k tH 30 3 pg 0 50 150 250 fL Review Quality control e 22 x 10 L Ly Service 1 0 fL T Calibration T 0 199 0 5 10 15 20 25 fL Shutdown Reagents for gt 99 Counts MENU T 1 lt 7 Select sub menu ENTER Enter sub menu Figure 7 5 Entering Settings screen Press ENTER to enter the Settings screen as Figure 7 6 shows Setup Ready 21 12 Settings Reagent Remains Remains Print amp comm Date amp time Diluent Gain Rinse yasla Count Container Ref range LYSE Other Exp Dat Diluent Rinse Lyse Reagents for gt 99 Counts MENU lt 7 Move cursor T Select setting item F1 Select setting group Figure 7 6 Settings screen 7 4 Auto Hematology Analyzer Operation Manual V1 00 Setup This screen is interpreted as follows 1 Setting Groups area This area displays the visible
19. 0 3 9 Sample Analysis Whole Blood All Enter sample information Cu ID 1 ID 00000119 Gender I Time Q Name Mode Age 000 Years Chart No go000000 Name Dept X Gender Sender Age Tester m Ne Checker ID 1 Mode Reagents for gt 99 Counts MENU Figure3 6 Select Gender 3 To enter the patient name you may A Press f or J to move the cursor the edit box right of Name B Use a keyboard to enter the patient name Press DEL if you want to delete the character after the cursor Press Backspace on the external keyboard if you want to delete the character before the cursor C When you are done entering the patient name press T or J to move the cursor to the next field to be edited 4 To enter the patient age Before entering the patient s age you should know that this analyzer provides three ways for you to do so entering the age in years in months and in days The first way is designed for the adult patients or pediatric patients no younger than one year old the second is designed for the infant patients older than one month old including one month and younger than one year old the third is designed for the neonatal patients no more than one month old You can choose only one of the three ways to enter
20. 1 3 9 8 Auto Hematology Analyzer Operation Manual V1 0 Troubleshooting 9 2 22 Elevator Motor Error Something is wrong with the motor that controls elevation of the sample probe Access Service gt Self test gt Machine and test the motor as instructed by Chapter 8 4 2 The error will be cleared if the result is normal Otherwise call Mindray Customer Service Department or the distributor 9 2 23 Real Time Clock Error Something is wrong with the clock Access Setup gt Settings gt Date amp Time and set the time and date as instructed by Chapter 7 2 3 and restart the analyzer to enable the new settings If the error still remains call Mindray Customer Service Department or the distributor 9 2 24 Scanner Error Too long or invalid bar code Check and make sure the bar code is correct If the error still remains call Mindray Customer Service Department or the distributor 9 2 25 Scanner Communication Error Something is wrong with the communication between the scanner and the analyzer Check the connection between the two devices 9 2 26 Communication Error The received communication settings are different with the BC 2800 Access Setup gt Settings gt Print amp comm and change the communication settings accordingly as instructed by Chapter 7 2 2 2 9 2 27 File Error Something is wrong with file saving Auto Hematology Analyzer Operation Manual V1 0 9 9 Troubleshooting Turn off t
21. 5 Other Lymph 40 0 RDW SD 0 56 0 Mid 9 0 PLT 300 Gran 70 MPY 7 0 11 0 HGB 60 PDW 15 0 17 0 RBC 5 50 PCT 108 282 HCT Group General F2 Save F4 Default Reagents for gt 99 Counts MENU 7 Move cursor f Select setting item F1 Select setting group Figure 7 32 Setting upper limit for WBC Press or gt to move the cursor within edit box Press PgUp or PgDn or the numeric keys on an external keyboard to enter a digit at the position where the cursor is located Press ft or to move the cursor to the lower limit of the parameter for instancet WBC whose reference range you want to change See Figure 7 33 7 24 Auto Hematology Analyzer Operation Manual V1 0 Setup Setup Ready 23 50 Reagent Para Lower Upper Para Lower Upper Print amp comm ywec 004 0 10 0 MCV 82 0 95 0 ate Stime Lymph 08 40 MCH 7 0 31 0 Count Mi d 0 1 0 9 MCHC 360 Ref range Grant 2 0 7 0 RDW CV 115 145 Other Lymph 20 0 0 RDW SD 35 0 56 0 Mid 3 0 9 0 PLT 300 Gran 50 0 0 MPV 7 0 11 HGB 110 PDW 15 17 RBC 3 50 60 PCT 282 HCT 37 0 Group General oeo00000 F2 Save F4 Default Reagents for gt 99 Counts MENU gt Move cursor 1 Select setting item F1 Select setting group Figure 7 33 Setting lower limit for WBC Press or gt to move the cursor within edit box Press PgUp or PgDn or the numeric
22. Chapter 3 Sample Analysis This section covers Power up Sample Analysis e OO E d Shutdown Preparing to start Preparing Samples Background check Entering Count Screen Entering Sample Information 3 1 Preparing to Start Sample Analysis Before turning on this analyzer you must perform the following checks to make sure that it is ready to run 3 1 1 Checking Reagents Refer to Table 3 1 and make sure there are enough reagents for the upcoming operation of this analyzer Table 3 1 Table of reagent consumption shutdown Diluent Rinse Lyse E Z Cleanser For one normal startup 46 5ml tubing cleaning 1 16 5ml 0 5ml procedure tubing background check cleaning background 75mli tubing cleaning 2 26ml 1ml check s background checks For one sample 28 5ml 9 5ml 0 5ml analysi WB AII mode For one sample 30 5mI including the 1 6 ml to 9 5ml 0 36ml analysis PB All mode predilute the sample For one normal 13 5ml 5 5 ml 1ml 3 1 2 Checking Waste Container You should prepare a container to receive the waste discharged by this analyzer Be sure to empty the container after every time you Shutdown this analyzer Auto Hematology Analyzer Operation Manual V1 0 Sample Analysis Biohazard Consider all materials specimens reag
23. Count The parameter units and count time are visible to all users but changeable only to the administrators See Figure 7 27 You may follow the instructions given below to view or change those settings Setup Ready 23 47 Settings Reagent Group member Print amp comm Lymph Mid Grant Date amp time Mid Gran Gain Count Ref range Other MCHC MCH RDVW SD HCT PDW PCT Count t WBC RBC u Reagents for gt 99 Counts MENU lt 7 Move cursor T Select setting item F1 Select setting group Figure 7 27 Setting units and count time 7 2 5 1 Setting parameter units This analyzer provides multiple units for certain parameters Refer to Table 7 3 for all the selectable units for all parameters The 19 parameters are divided into 11 groups based on their units and you can only select unit for the first parameter of a group Pay special attention to the HGB group which includes HGB MCHC and MCH When you select g L or g dL as the unit of HGB the default unit for MCH is pg and when you select mmol L as the unit of HGB the default unit of the MCH is fmol Table 7 3 Units of Parameters Display Unit Remarks Parameter
24. F1 Edit F2 X B Graph Figure 4 15 X B Table Screen At the screen if you want to acquire help information press HELP If you want to print out the existing X B results by an external printer press PRINT You cannot print the X B results by the built in recorder If you want to delete the displayed X B results press DEL and confirm the deletion 4 2 2 Editing X B Settings At the X B Table screen press F1 to enter the X B Edit screen as shown in Figure 4 16 X B analysis Ready 15 00 X B Table X B Edit X B analysis m Para Mean Range MCV MCH Figure 4 16 X B edit screen Reagents for gt 99 Counts MENU Auto Hematology Analyzer Operation Manual V1 0 4 13 Quality Control 1 Enabling disabling X B analysis A Press ft or to move the cursor to the combo box right of X B analysis B Press ENTER to display the pull down menu as Figure 4 17 shows X B analysis Ready 15 00 X B Edit X B analysis Para MCV MCH Reagents for gt 99 Counts MENU Figure 4 17 Eabling X B analysis C Press f or to move the cursor to the On Off and press ENTER to enable or disable the X B analysis 2 Entering expected results and ranges To include a param
25. F3 to enter the Transmission screen as Figure 6 17 shows At this screen you may Press T or to move the cursor to Selected and press ENTER to transmit the Auto Hematology Analyzer Operation Manual V1 0 6 11 Sample Review selected sample results to an external computer Or Press T or J to move the cursor to All and press ENTER to transmit all the saved sample results in either the Sample or Searched database to an external computer Or Press T or J to move the cursor to Stop and press ENTER to stop the transmission Or Press T or J to Exit to return to the Review screen Review Ready 20 53 Reagents for gt 99 Counts MENU Figure 6 17Transmission screen 6 2 5 Searching Results If you want to search the Sample database for the certain results press F4 at the Review screen to enter the Search screen as Figure 6 18 shows specify any of the 7 search conditions listed in Figure 6 18 Follow the steps given below to Review Ready 20 54 ame Gender Dept D Bed No Chart No Date oooo0000 oooo0000 oooo0000 on N 0O OO Start 126 1 v Reagents for gt 99 Counts Figure
26. Q Name i ka Mode Age 000 Years oo Months foo Days pO fL Chart No 90000000 Bed No 0000000 Name Dept X Gender Sender x Age Tester 0 fL Ne Checker X iD 1 Yes Mo Mode r Per 0 199 0 5 10 15 20 25 fL Figure 3 5 Entering sample information To enter the sample ID you may A Press f or J to move the cursor to the edit box right of ID as Figure 3 5 shows B Press or gt to move the cursor within the edit box Press PgUp or PgDn or the numeric keys of a keyboard to enter a digit at the position where the cursor is located To modify an entered digit you can move the cursor to the corresponding location and re enter the desired digit If a bar code scanner is available you present the bar code to the scanner and the scanner buzzes when the scanning is done When you are done entering the sample ID press T or to move the cursor to the next field to be edited 2 To enter the gender of the patient you may A Press T or J to move the cursor to the edit box right of Gender as Figure3 6 shows Press ENTER to display the pull down menu as Figure3 6 shows Press T or 4 to select Male for the male patient or Female for the female patient or blank for the patient whose sex you are unaware of Press ENTER to confirm the selection Press T or J to move the cursor to the next field to be edited Auto Hematology Analyzer Operation Manual V1
27. The external computer must respond by sending an ACK 06 Hex If the external computer responds by sending a NACK 15 Hex then BC 2800 will execute Step 3 once again If anything else is received BC 2800 will send ETX 03 Hex once more A 4 2 Sample Date Format Sample Data Format as shown in Table A 2 Table A 2 Sample Data Format If handshake is selected ENQ If handshake is not selected STX Body of the text start Text Distinction Code A ID FEA Sample Mode Month Day Year Hour Minutes Seconds WBC 107 L Lymph 10 L HHH tinier we Auto Hematology Analyzer Operation Manual V1 0 Communication Mid 10 L HHH Gran 10 L HHHH Lymph HHH Mid HHH Gran HHA RBC 10 7 L HHH HGB g L HHH MCHC g L HHHH MCV fL HHHH MCH pg HHH RDW CV HHH HCT HHH PLT 10 L HHHH MPV fL HHH PDW HHH PCT HH RDW SDf fL HHHH Reserved FHA Rm R1 R2 R3 R4 Pm Ps Pl L1 Region HHH L2 Region HHH L3 Region HHH L4 Region HHH L5 Region HFH L6 Region HHH L7 Region HHH L8 Region HHH Reserved AHAHHH HRHHHHHHHH WBC Histo 256 channels RBC Histo 256 channels A 4 Auto Hematology Analyzer Operation Manual V1 00 Communication PLT Histo 256 channels HHH Body of the text end If handshake is selected EOT If handshake is not selected EOF For all the data formats if the data are mark in BC 2800 then 2A Hex w
28. Wear standard laboratory attire glove and follow safe laboratory f procedures when handling any material in the laboratory 8 4 Auto Hematology Analyzer Operation Manual V1 00 Maintenance You should perform the Rinse prime procedure to prime the rinse tubing with the rinse when there are bubbles in the tubing or the rinse in the tubing is contaminated or the old rise ran out and a new container of rinse is installed Follow the steps given below to do so At the Maintenance screen press the appropriate arrow keys T gt to move the cursor to Rinse prime Press ENTER to prime the tubing with rinse and the priming progress will be displayed at the bottom of the screen as Figure 8 4 shows e When the priming is done the screen will return to the initial state Service Ready 23 58 Maintenance Item Diluent prime Lyse test Rinse prime Clean bath Lyse prime Drain bath Zap aperture Drain tubing Flush aperture Clean wipe block Probe cleanser cleaning Prepare to ship E Z cleanser cleaning Priming rinse Rinse prime PERE RRRRRRRR EERE 64 Reagents for gt 99 Counts MENU T 1 lt 7 Select service item ENTER Execute Figure 8 4 Priming tubing with rinse 8 1 3 Lyse Prime Biohazard FON Wear standard laboratory attire glove and follow safe laboratory D 4 procedures when handling any material in the laboratory You shoul
29. Width Standard Deviation fL is set on the 20 frequency level with the peak taken as 100 as Figure 1 4 shows 100 20 ee RDW SD Figure 1 4 RBC Distribution Width Standard Deviation 1 4 4 9 PLT PLT 10 L is measured directly by counting the platelets passing through the aperture 1 4 4 10 MPV Based on the PLT histogram this analyzer calculates the mean platelet volume MPV fL 1 4 4 11 PDW Based on the PLT histogram this analyzer calculates the platelet distribution width PDW 1 4 4 12 PCT This analyzer calculates the PCT as follows 9 PCT PLT 10 L x MPV fL 10000 1 10 Auto Hematology Analyzer Operation Manual V1 0 Introduction 1 5 Specifications 1 5 1 Parameter Description Table 1 3 Directly measured parameters and histograms Parameter Abbreviation Default Unit White Blood Cell or leukocyte WBC 10 L Hemoglobin Concentration HGB g L Red Blood Cell or erythrocyte RBC 10 L Platelet PLT 10 L WBC histogram RBC histogram PLT histogram Table 1 4 Parameters derived from histograms Parameter Abbreviation Default Unit Lymphocyte percentage Lymph Mid sized cell percentage Mid Granulocyte percentage Gran Mean Corpuscular erythrocyte Volume l i k Man Red Blood Cell erythrocyte Distribution Width RDW CV Coefficient of Variation Red Blood Cell erythrocyte Distributio
30. any instrument from which Mindray s original serial number tag or product identification markings have been altered or removed or any product of any other manufacturer Return Policy Return Procedure In the event that it becomes necessary to return a unit to Mindray the following procedure should be followed 1 Obtain return authorization Contact the Mindray Service Department and obtain a Customer Service Authorization Mindray number The Mindray number must appear on the outside of the shipping container Return shipments will not be accepted if the Mindray number is not clearly visible Please provide the model number serial number II Auto Hematology Analyzer Operation Manual V1 00 and a brief description of the reason for return 2 Freight policy The customer is responsible for freight charges when equipment is shipped to Mindray for service this includes customs charges Company Contact Address Mindray Building Keji 12th Road South Hi tech Industrial Park Nanshan Shenzhen 518057 P R China Free hot line 86 800 830 3312 Phone 86 755 26582888 Fax 86 755 26582680 Auto Hematology Analyzer Operation Manual V1 00 Ill Safety Symbols Symbol Warning Condition Action Biohazard Consider all materials specimens controls calibrators or components that contain or have contacted blood as infectious human being potentially Wear standard laboratory attire glove and follo
31. e PL PM PG Mid x 100 PL PM PG PG Gran x100 PL PM PG where PL particles in the lymphocyte region 10 L PM particles in the mid size region 10 L PG particles in the granulocyte region 10 L Having achieved the three parameters above this analyzer proceeds to calculate the Lymph Mid and Gran as follows Lymph x WBC 10 I L Lymph 10 L nn l 100 j 9 Masao iros ee 100 9 Gran 10 L Gran x WBC 10 L 100 1 4 4 3 HGB Using the colorimetric method this analyzer calculates hemoglobin concentration g L as follows HGB g L Constantx Log 49 Blank Photocurrent Sample Photocurrent 1 4 4 4 RBC RBC 10 7 L is the number of erythrocytes measured directly by counting the erythrocytes passing through the aperture 1 4 4 5 MCV Based on the RBC histogram this analyzer calculates the mean cell volume MCV and expresses the result in fL Auto Hematology Analyzer Operation Manual V1 00 1 9 Introduction 1 4 4 6 HCT MCH and MCHC This analyzer calculates the HCT MCH pg and MCHC g L as follows RBC 10 L x MCV fL HCT 10 HGB g L MCH zene oe p8 RBC 10 L MCHC g L HOBBEL 00 HCT 1 4 4 7 RDW CV Based the WBC histogram this analyzer calculates the CV Coefficient of Variation of the erythrocyte distribution width 1 4 4 8 RDW SD RDW SD RBC Distribution
32. first collect 20u L capillary specimen and dispense 1 6mL diluent from this analyzer to predilute the specimen Then the operator should present the prediluted sample to the analyzer which will aspirate 0 7ml of the sample for further dilution as Figure 1 2 shows Capillary Blood Sample 20uL Diluent 1 6mL 1 1 Dilution Diluent 2 1mL 1 324 Dilution Diluert O44mL Diluent 3mL Lyse 0 36mL For WBC Analysis 1 417 Dilution For RBC Analysis 1 45004 Dilution Figure 1 2 How a prediluted sample is diluted After reacting with the diluent and lyse the cells mainly fall into the following three volume ranges WBC 300 350 fL RBC 25 250 fL PLT 20 30 fL 1 4 3 Control of Sample Volume This analyzer employs an optical volumetric metering system to determine the sample volume aspirated through the aperture Each count cycle as shown in Figure 1 3 starts when the liquid surface passes the upper sensor of the volumetric tube and ends till the lower sensor is reached If bubbles or an abnormal flow rate is detected the system will alarm the operator for errors When this happens see Chapter 9 Troubleshooting for the solutions Auto Hematology Analyzer Operation Manual V1 00 1 7 Introduction Upper sensor Upper sensor Lower sensor Lower sensor 1 Empty when start 2 Liquid surface fall down through the volumeritc tube Upper sensor Upper sensor i Lower sens
33. give an alarm without stopping running Relative humidity 30 85 Atmospheric pressure 60 0kPa 106 0kPa 1 5 13 Transportation and storage environment Ambient temperature 10 C 40 C Relative humidity 10 93 Atmospheric pressure 50 0kPa 106 0kPa 1 5 14 Dimension Width Height Thickness 32 2cm 43 7cm 39 7cm 1 5 15 Weight Net Weight 17 9KG Gross Weight 25 2 KG 1 14 Auto Hematology Analyzer Operation Manual V1 00 Introduction 1 6 System Operation 1 6 1 Main Unit val y _ gt a __ amp A Figure 1 5 Front view 1 LCD 2 Keypad 3 Recorder 4 Power Indicator 5 START key 6 Sample Probe Auto Hematology Analyzer Operation Manual V1 00 1 15 Introduction i N NN r a n E oA or e so NS 2 5 E Figure 1 6 Back view 1 Power switch 2 Equipotentiality 3 Waste Outlet Red 4 Lyse Inlet Orange 5 Rinse Inlet Blue 6 Diluent Inlet Green 1 16 Auto Hematology Analyzer Operation Manual V1 00 Introduction z c A Figure 1 7 Left view 1 RS232 Port1 for the scanner 2 Parallel also for the floppy disk drive 3 RS232 Port 2 for the computer 4 Power Interface of Floppy Disk Drive 5 Keyboard Interface
34. given below to return to the X B Table screen A Press MENU and a dialog box will pop up to remind the user to save the changes as Figure 4 18 shows You may X B analysis Ready 15 02 X B Table X B Edit oP analycic fat Save new X B results Le MCAT 320 DUST Figure 4 18 Dialog Box to Confirm the Changes Reagents for gt 99 Counts MENU B Press or gt to move the cursor to Yes and press ENTER to save the changes and exit Or C Press lt or to move the cursor to No and press ENTER to exit without saving the changes 4 Other operations A You must empty the X B table before editing the X B settings B If you neither entered new settings nor changed the old settings you may press MENU to directly return to the X B Analysis screen without seeing the dialog box shown in Figure 4 18 If you want to print out the saved X B settings press PRINT Note newly entered or changed settings will not be printed out until they are saved Auto Hematology Analyzer Operation Manual V1 0 4 15 Quality Control 4 2 3 Running X B Analysis After you have enabled the X B analysis and assigned valid means and ranges to the three parameters the system will automatically run the X B analysis every 20 patient samples of which the MCV MCH and MCHC results are within the set ranges 4
35. gt 99 Counts MENU f Searched Histogram F1 Goto F2 Select F3 Transmit F4 Search Figure 6 14 Discontinued samples de selected 6 2 3 Deleting Selected Results If you want to delete the selected sample results follow the steps given below to do so At the Review screen press DEL and a dialog box shown will pop up to confirm the deletion as Figure 6 15 shows You may Press lt or gt to move the cursor to Yes and press ENTER to confirm the deletion Or Press or gt to move the cursor No and press ENTER to abort the deletion 6 10 Auto Hematology Analyzer Operation Manual V1 00 Sample Review Review Ready 20 52 Loc Total Reagents for gt 99 Counts MENU Figure 6 14 Confirm deletion Note that you can only delete the selected sample results If you press DEL with no sample results selected a dialog box will pop up to remind you to select sample results as Figure 6 16 shows You need to press ENTER to return to the Review screen and follow the steps mentioned above to select the sample results to be deleted Review Ready 20 52 Reagents for gt 99 Counts MENU Figure 6 15 Remind you to select samples first 6 2 4 Transmission If you want to transmit sample results to an external computer follow the steps given below to do so At the Review screen press
36. note down the preset WBC count time Access Service gt Self test gt Tubing and test the actual WBC count time as instructed by Chapter 8 4 1 If the tested time differs from the preset time by less than 2 seconds it means the unclogging is successful and you can return to the Count screen to continue the analysis Otherwise Access Service gt Maintenance to soak the bath and tubing with probe cleanser as instruction by Chapter 8 2 6 When the soaking is done access Setup gt Settings gt Count to note down the preset WBC count time Access Service gt Self test gt Tubing and test the actual WBC count time as instructed by Chapter 8 4 1 If the tested time differs from the preset time by less than 2 seconds it means the unclogging is successful and you can return to the Count screen to continue the analysis If the difference is still greater than 2 seconds and stabilized around a certain value access Setup gt Settings gt Count to change the WBC count time accordingly After the adjustment test the actual count time again and make sure the difference is within 2 seconds 3 If the error still remains call Mindray Customer Service Department or the distributor Auto Hematology Analyzer Operation Manual V1 0 9 5 Troubleshooting 9 2 8 WBC Bubbles This error message occurs when the actual WBC count time is less than the preset WBC count time by 2 seconds Possible causes 1 Insufficient diluent or rinse 2
37. reproducibility is within the range specified in Table 5 1 Table 5 1 Reproducibility Parameter Condition CV WBC x107 L 4 0 7 5 lt 3 0 7 6 15 0 lt 2 0 RBC x10 L 3 00 4 50 lt 2 0 4 51 6 50 lt 1 5 HGB g L 110 180 lt 15 MCV fL 70 0 100 0 lt 1 0 PLT x10 L 100 200 lt 5 0 201 500 lt 40 4 Mindray recommends a record table which may include date calibrator information name lot number and mean and background check results be prepared to keep the calibration results When are done with the preparation you may choose one or several of the following parameters for calibration WBC RBC HGB MCV and PLT The calibration should be conducted by the administrators see Section 7 1 1 rather than the common users The calibration range of the calibration factors of the BG 2800 is 75 125 5 2 Auto Hematology Analyzer Operation Manual V1 0 Calibration 5 3 Manual Calibration 5 3 1 Calibration 1 Press MENU to enter the system menu Press the appropriate arrow keys to move the cursor to Count screen as Figure 5 1 shows Count Whole Blood All Ready 13 40 100 200 300 fL Review Quality control gt Setup gt Service gt Calibration Shutdown Reagents for 67 Counts MENU T 1 lt 7 Select sub menu ENTER Enter sub menu Figure 5 1 Entering count screen 2 Press
38. s manual of the scanner for more details The scanner buzzes when the scanning is done 2 Ifthe bar code scanner is not available you may follow the steps given below to enter the sample ID from the keypad or external keyboard At the Count screen press F1 to enter the Next sample screen as Figure 3 3 shows Current sample WBC 0 0 x 10 L Lymph eee te i LT A D I Mid ree T W i Rae Gran eee ex 10 L p i Time 01 26 2004 21 14 Lymph hide f i Mid wet T T T T T T Mode AGEA oe 0 100 200 300 fL Next sample i Name RB ID HC 00000001 Gender MC i T T T T T Age MG pg 0 50 150 260 fL MCHC eeee g L Next sample RDW CV rE i RDVV SD are fh pl ID 1 PLT Ox 10 L T l MPY TRA GL i All i i Mode WB PDW RA e S PCT pets 06 Oo 5 10 16 20 25 fL Reagents for gt 99 Counts MENU PgUp PgDnjEnter digits ENTER Exit Figure 3 3 Entering ID of the next sample Press lt or to move the cursor within the edit box right of ID Press PgUp or PgDn or the numeric keys on a keyboard to enter a digit at the position where the cursor is located When you are done entering the sample ID press ENTER to save the changes and the entered number will be displayed in the ID field of the Next sample area of the Count screen If you want to ignore the entered number for any reason you may Auto Hematology Analyzer Operation Manual V1 0 3 7 Sample
39. still remains call Mindray Customer Service Department or the distributor 9 2 6 RBC Bubbles This error message occurs when the actual RBC count time is less than the preset RBC count time by 2 seconds Possible causes 1 Insufficient diluent or rinse 2 Loose tubing connection 3 Inappropriate RBC count time setting Solution 1 Check if the diluent or rinse is sufficient If not change a new container of diluent of rinse as instructed by Chapter 2 3 2 Check the tubing connections If necessary reconnect the tubing as instructed by Chapter 9 4 Auto Hematology Analyzer Operation Manual V1 0 Troubleshooting 2 3 3 If the error still remains Access Setup gt Password to gain the administrator authority as instructed by Chapter 7 1 1 and then access Setup gt Settings gt Count and adjust the RBC count time as instructed by Chapter 7 2 5 2 4 If the error still remains turn off this analyzer and call Mindray Customer Service Department or the distributor 9 2 7 WBC Clog This error message occurs when the actual WBC count time is greater than the preset WBC count time by 2 seconds Possible causes clogged aperture inappropriate WBC count time settings or solenoid valve error Solution 1 Access Service gt Maintenance and do the Zap aperture and Flush aperture procedures as instructed by Chapters 8 2 4 and 8 2 5 2 After unclogging access Setup gt Settings gt Count to
40. the patient age A To enter the patient age in years you may a Press T or to move the cursor to the edit box left of Year b Press or gt to move the cursor within the edit box Press PgUp or PgDn or the numeric keys of a keyboard to enter a digit at the position where the cursor is located To modify an entered digit you can move the cursor to the corresponding location and re enter the desired digit Note that you can only enter a positive integer no greater than 255 in this edit box c When you are done entering the patient age press f or to move the cursor to the next field to be edited 3 10 Auto Hematology Analyzer Operation Manual V1 0 Sample Analysis To enter the patient in months you may Press f or to move the cursor to the edit box left of Month Press or to move the cursor within the edit box Press PgUp or PgDn or the numeric keys of a keyboard to enter a digit at the position where the cursor is located To modify an entered digit you can move the cursor to the corresponding location and re enter the desired digit Note that you can only enter a positive integer no greater than 12 in this edit box When you are done entering the patient age press f or to move the cursor to the next field to be edited To enter the patient age in days you may Press f or to move the cursor to the edit box left of Day Press or to mov
41. the recording paper 1 Follow the arrow in the figure below to open the door of the recorder as Figure 2 4 shows Figure 2 4 Open the recorder door 2 Lift the press bar as Figure 2 5 shows Figure 2 5 Lift the press bar 3 Insert recording paper into the paper entry as Figure 2 6 shows Figure 2 6 Load recording paper 2 6 Auto Hematology Analyzer Operation Manual V1 00 Installation 4 Push the press bar back and close the recorder door as Figure 2 7 shows Figure 2 7 Close recorder door Auto Hematology Analyzer Operation Manual V1 00 2 7 Installation 2 5 Installing Printer If your analyzer is equipped with a printer use the printer cable to connect it to the interface marked PARELLEL on the left of this analyzer See the printer s user s manual for installation details 2 8 Auto Hematology Analyzer Operation Manual V1 00 Installation 2 6 Installing Scanner optional The COM1 of the BC 2800 is reserved for the bar code scanner If your analyzer is equipped with the scanner follow the scanner s operation manual to connect it to the BC 2800 You need to set the scanner if it is your first time using it The settings will be saved to the system and you only have to set it once Refer to the scanner s operation instruction to set the Baud rate to 9600 Data bit to BIT 8 Parity to EVEN and Handshaking to NONE Auto Hematology Analyzer Operation Manual V1 00 2 9
42. times n 5 and the results are 8 1 8 00 8 1 8 1 and 8 3 abe Mean Diff l 40 012 x 0 11 n l CV DU 100 z 1 3 lt 2 ean Since the calculated CV is less than 2 the mean value 8 12 is valid and the new calibration factor can be calculated as follows newfactor 102 3 Press F2 to enable the edit boxes right of the 5 parameters as Figure5 5 shows Auto Hematology Analyzer Operation Manual V1 0 5 5 Calibration Calibration Manual Ready 15 48 Whole Blood Factor Time O Prediluted 02 0 oo oo oo 0 100 0 0 0 01 27 2004 01 27 2004 01 25 2004 01 25 2004 01 27 2004 Reagents for gt 99 Counts MENU F2 Exit Figure5 5 Editing calibration factors Detailed operation Press f to switch among the factors press lt on the keypad to move the cursor within the edit box press PgUp PgDn to change the digits Use the numeric keys on a keyboard to enter the desired number directly 4 Exiting When press F2 to quit editing the factors the analyzer will judge whether the factors have been changed If nothing was changed it will directly go back to the Manual screen If there are some changes and the entered number is out of the calibration range a dialog box will pop up to remind you
43. to select one of the six analysis modes The selected mode will be displayed in the Count Mode area 1 WB AII It stands for the Whole Blood All Parameter mode meaning the sample to be analyzed is a whole blood sample and all the 19 parameters are to be analyzed 2 WB WBC HGB It stands for the Whole Blood WBC HGB Group mode meaning the sample to be analyzed is a whole blood sample and only the following parameters are analyzed WBC Lymph Mid Gran Lymph Mid Gran and HGB plus the WBC histogram 3 WB RBC PLG It stands for the Whole Blood RBC PLC Group mode meaning the sample to be analyzed is a whole blood sample and only the following parameters are analyzed RBC HCT MCV MCH MCHC RDW CV RDW SD PLT MPV PDW and PCT plus the RBC and PLT histograms 4 PB All It stands for the Prediluted Blood All Parameter mode meaning the sample to be analyzed is a prediluted blood sample and all the 19 parameters are to be analyzed plus 3 histograms 5 PB WBC HGB It stands for the Prediluted Blood WBC HGB Group mode meaning the sample to be analyzed is a whole blood sample and only the following parameters are analyzed WBC Lymph Mid Gran Lymph Mid Gran and HGB plus the WBC histogram 6 PB RBC PLG It stands for the Prediluted Blood RBC PLC Group mode meaning the sample to be analyzed is a prediluted blood sample and only the following parameters are analyzed RBC HCT MCV MCH MCHC RD
44. tol BL Time 01 24 2004 13 55 Lymph H 41 0 i i i Mid 8 0 WB All 1 f Made B Gran 510 0 oo 200 300 fL HGB 24 g L Name RBC 4 08 x 102 L HCT L 36 0 Gender MCV 88 3 fL i Age MCH 30 3 pg 0 50 150 250 fL MCHC 344 g L Next sample RDW CV 13 6 RDVV SD 40 2 fL ID 119 PLT 222 x 10 L 7 i MPY 9 0 fL f Mode WB All PDW 157 i PCT 0 199 0 5 10 16 20 25 fL Figure3 13 Adjusting WBC histogram 1 4 Press to shift the discriminator to 100fL as Figure3 14 shows Whole Blood AlIl 3 a Current sample WBC 8 1 x 10 L igi i Lymph 3 7 x 10 L i Mid 0 5 x 10L W ID 118 Gran 4 9 x10 Bf Time 01 24 2004 13 55 Lymph H 41 1 3 i Mid 4 8 WB All f Mode B Gran 5A 1 0 100 200 300 fL HGB 24 g L Name RBC 4 08 x 1012 L HCT L 36 0 Gender MCV 88 3 fL i Age MCH 30 3 pg 0 50 150 250 fL MCHC 344 g L Next sample RDW CV 13 6 i RDW SD 40 2 fL P i ID 119 PLT 222 x 10 L 7 i MPY 9 0 fL f Mode WB All PDW 157 l i PCT 0 199 0 65 10 15 20 25 fL Figure3 14 Adjusting WBC histogram 2 5 Press ENTER to finish the adjustment and a dialog box will pop up to remind you to save the adjustment as Figure3 15 shows Auto Hematology Analyzer Operation Manual V1 0 3 23 Sample Analysis Whole Blood All Current sample WBC 9 1 x 10 L Lymph 3 7 X 10 L Mi d 0 5 x10 W ID 118 Gran 4 9 x10 Bf Time 01 24
45. within edit box f Move cursor between items Figure 3 8 Batch edit screen 2 Follow the instructions given in section 3 4 2 All Info Mode to enter the information of the first sample 3 Press the appropriate arrow keys to move the cursor to Save and press ENTER to save the changes 4 Repeat the last two steps until you are done with the batch 5 If you want to review the entered information of a specific sample press the appropriate arrow keys to move the cursor to Prev or Next and press ENTER to review the information of the previous or next sample until the desired sample is reached 6 If you want to delete the currently displayed sample information press the appropriate arrow keys to move the cursor to Delete and press ENTER to complete the deletion 7 When you are done with the batch press the appropriate arrow keys to move the cursor to Exit and press ENTER to exit to the Count screen After a sample is analyzed its corresponding sample information will be automatically displayed in the Current sample area 3 14 Auto Hematology Analyzer Operation Manual V1 00 Sample Analysis 3 5 Preparing Samples BC 2800 is capable of analyzing two types of samples whole blood samples and prediluted samples Biohazard Consider all materials specimens reagents controls calibrators or components that contain or have contacted human blood as being potentially infectious Wear standard laboratory attire glove
46. 1 00 Calibration 5 4 2 Editing Calibration Settings 1 Atthe Auto calibration screen press F1 to select the desired calibration mode 2 Press F2 to enable the edit boxes as Figure5 10 shows Calibration Ready 15 55 Exp Date 06 10 2004 Whole Blood 1 2 3 4 5 O Prediluted Factor Reagents for gt 99 Counts MENU F2 Exit Figure5 10 Editing reference values for the parameters to be calibrated 3 Follow the instruction below to edit the values refer to the instruction manual of the calibrator for such information as lot number expiration date and reference values Entering lot number Press f or to move the cursor to the edit box right of Lot No Press or to move the cursor to the desired position within the box Press PgUp or PgDn or the numeric keys of a keyboard to enter a digit at the position where the cursor is located To modify a digit at a certain position just move the cursor to that position and re enter the desired digit Entering expiration date Press f or to move the cursor to the edit box right of Exp Date press PgUp or PgDn to enter the digits for the expiration date in the MM DD YYYY format Entering reference To include a parameter into the upcoming calibration you must first set a reference value for it Move the cursor to corresponding edit box in the Mean column and enter the ex
47. 10 Auto Hematology Analyzer Operation Manual V1 0 Troubleshooting Solution Access Service gt Self test gt Tubing and test the pressure as instructed by Chapter 8 4 1 The error will be cleared if the result is normal otherwise call Mindray Customer Service Department or the distributor 9 2 33 Vacuum Error The system does not reach the expected vacuum within the given time Solution Access Service gt Self test gt Tubing and test the vacuum as instructed by Chapter 8 4 1 The error will be cleared if the result is normal otherwise call Mindray Customer Service Department or the distributor 9 2 34 Syringe Motor Error Something is wrong with the motor controls the syringe that aspirates dispenses samples and reagents Solution Access Service gt Self test gt Machine and test the motor as instructed by Chapter 8 4 2 The error will be cleared if the result is normal otherwise call Mindray Customer Service Department or the distributor Auto Hematology Analyzer Operation Manual V1 0 9 11 Communication Appendix A Communication O BC 2800 Communication Protocol The BC 2800 can transmit the sample data and QC data to an external computer through its RS 232C serial port The transmission can be conducted either automatically or through the command of the operator after the completion of the sample analysis This section gives detailed discussion about the setup of transmission parameter RS 232C Ser
48. 2 4 Reviewing X B Results This analyzer automatically saves the results of the X B analyses You can review the saved results in two modes X B graph and X B Table 4 2 4 1 X B Table Follow the steps introduced in section 4 2 1 Entering the X B Analysis Screen 4 2 4 2 X B Graph At the X B Table screen press F2 to enter the X B Graph screen as Figure 4 19 shows For every parameter the X B graph presents 500 points 30 for every screen You can press PgUp or PgDn to view the previous or next screen At every screen you can press lt or to view the results of every point displayed in the box below the parameter name one by one X B analysis Ready 14 58 a ed ec a k et ee eee i TOPET a EEE SAAN n ae 7 Time 01 25 2004 16 31 Loc Total 1 25 Reagents for gt 99 Counts MENU gt Move cursor PgUp PgDn Scroll Figure 4 19 X B graph Screen X B Graph 1 The x coordinate represents how many times the QC program has been run The y coordinate represents the analysis results of the displayed parameters 2 For every parameter the upper dash line of its X B graph represents the upper limit of the expected range of the analysis result The corresponding value 100 in case of the MCV in Figure 4 19 equals Mean Range and is displayed on the left of the line 4 16 Auto Hematology Analyzer Operation Manual V1 00 Quality Control 3 For every para
49. 2004 13 55 Lymph H 41 1 i i Mid 4 8 Mode WB All O 100 200 300 fL i Gran 54 1 Name Save adjustment i Gender Yi a M Next sample RDW CV 13 6 RDW SD 40 2 fL ID 119 PLT 222 x TOSE z i MPY 9 0 fL i Mode WB AIl PDW 157 PCT 0 199 0 6 10 15 20 25 fL Figure3 15 Saving changes 6 To save the adjustment move the cursor to Yes and press ENTER otherwise move the cursor to No and press ENTER 3 24 Auto Hematology Analyzer Operation Manual V1 0 Sample Analysis 3 7 Background Check The system will check the background during the startup procedure or when the ID of the next sample is 0 and the START key is pressed The background check refers to the analysis in which the sample is replaced by the diluent Result of the background check should meet the following requirements and if not the system will give an alarm for the Background abnormal error Table 3 4 Background check requirements Parameter Expected results WBC lt 0 3x 10 L and not invalid results RBC lt 0 03 x 10 LD and not invalid results HGB lt 1g L j and not invalid results HCT lt 0 5 and not invalid results PLT lt 7 x 10 LQ and not invalid results Auto Hematology Analyzer Operation Manual V1 0 3 25 Sample Analysis 3 8 Shutdown Note To ensure the sta
50. 26 2004 21 14 Lymph ort i Mid str T T T T T T 1 Mode io oo 0 100 200 adot HGB 0 g L Name R RBC o o0 x 102i BN HCT 0 0 eli count A e pg Coun ij ane t T T T T pH pg 0 50 150 250 fL Review FHC teer g L DWC eet i Quality control gt WW SD weet fL E Sere gt i 0x10 LI Service gt hy wee fL Tij Sa iiration hr ti o 0 5 10 15 20 25 fl Shutdown Reagents for gt 99 Counts gt Select sub menu ENTER Enter sub menu Figure 3 1 Entering Count screen from the system menu Press ENTER to enter the Count screen as Figure 3 2 shows Whole Blood All Current sample WBC 0 0 x 10 L Lymphit meee x 10 L D 0 Mid tea e x IDL w ID Grand ee OE R Time 01 26 2004 21 14 Lymph ae RO i Mid tt T ro Mode D 100 200 300 fL a Gran ee i HGB 0 g L Name RI RBC 0 00 x 1012 L B HCT 0 0 eli Gender MCV aat i ttt t T T T T Age MCH pg 0 50 150 250 fL MCHC wrr ok Next sample RDW CV EE RDW SD erm tL pli ID 1 PLT 0 x 10 L MPV tee FL l Mode WB All i i i PDW pee T T T T 4 PCT eet OG 0 6 10 15 20 25 fL MENU MODE Select count mode F1 Next sample F2 Unclog F3 St Figure 3 2 Count screen 3 3 1 Title Area The Title area displays the title of the current screen which in case of Figure 3 2 is Count 3 4 Auto Hematology Analyzer Operation Manual V1 0 Sample Analysis 3 3 2 Count Mode Area The Count Mode area displa
51. 3 9 6 Auto Hematology Analyzer Operation Manual V1 0 Troubleshooting 9 2 11 Printer Out of Paper Possible cause The printing paper ran out or is not correctly installed Check the whether the printer is out of paper If so load paper to the printer otherwise re install the existing paper 9 2 12 Printer Connection Error Check whether the printer is well connected to the analyzer 9 2 13 Waste full The waste container is full Empty the container as instructed by Chapter 2 3 or change a new container to receive the waste and re set the waste container volume as instructed by Chapter 7 2 1 2 9 2 14 Filter error Something is wrong with the filter of the vacuum chamber Solution Access Service gt Self test gt Tubing and test the filter as instructed by Chapter 8 4 The error is cleared if the result is normal If not call Mindray Customer Service Department or the distributor 9 2 15 Environmental Temperature Abnormal Possible causes jabnormal environmental temperature or malfunctioning temperature sensor Solution Access Service gt Status and check the environmental temperature If the temperature exceeds the specified range by 15 C 35 C you need to adjust the work environment of this analyzer so that the analyzer works in the requested environment If the temperature is within the requested range and the error remains call Mindray Customer Service Department or the distributor 9 2 16 Recor
52. 6 18 Search conditions 6 12 Auto Hematology Analyzer Operation Manual V1 0 J gt e o gt e 0o oo e N e o0 Sample Review To specify the name of the patient you may Press T or J to move the cursor to the check box left of Name Press ENTER to tick the box Press J to move the cursor to the edit box right of Name and use an external keyboard to enter the name of the patient to be searched Press 1 to move the cursor to the next search condition to be specified To specify the Gender of the patient you may Press fT or J to move the cursor to the check box left of Gender Press ENTER to tick the box Press l to move the cursor to the edit box right of Gender and press ENTER to display the pull down menu Press T or 1 to select Male for the male patient or Female for the female patient or blank for the patient whose gender you are unaware of Press T or J to move the cursor to the next search condition to be specified To specify the department from which the sample came you may Press f or J to move the cursor to the check box left of Dept Press ENTER to tick the box Press 1 to move the cursor to the edit box right of Dept Use an external keyboard to enter the name of the department This edit box automatically saves the entered item to its pull down menu which can be accessed by pressing ENTER You may press T or J t
53. 8 1 5 01 27 04 14 51 4 5 2 80 85 81 01 27 04 Reagents for gt 99 Caunts MENU F1 Transmit PgUp PgDn Scrall Figure 4 12 QC Table Screen If you want to delete all the saved results press DEL and confirm the deletion If you want to transmit the saved QC results to an external computer follow the steps given below A Press F1 to enter the dialog box shown in Figure 4 13 B Press lt or gt to move the cursor to Yes and press ENTER to confirm the transmission Or C Press lt or gt transmission to move the cursor to No and press ENTER to cancel the Contrals Ready 14 18 File No 1 Lot No 7645 Exp Date 07 27 2004 Mean Range 1 2 3 4 5 mi ime a wec RBC HGB PLT g Lymph Lymph Gran Gran g HCT MCV MCH MCHC 01 27 04 14 47 4 5 017 01 27 04 14 49 4 5 S04 01 27 04 14 51 4 5 a nan Yes Transmit 33 2 23 7 84 5 30 2 358 Reagents for gt 99 Counts MENU Figure 4 13 Transmission dialog box Auto Hematology Analyzer Operation Manual V1 0 Quality Control 4 2 X B Analysis The X B represents the moving average of hematology values calculated using an algorithm developed by Dr Brian Bull The X B analysis uses the Bull algorithm to monitor the p
54. 8 12 Auto Hematology Analyzer Operation Manual V1 0 Maintenance cleanser Remove the cleanser after the probe has risen up Unscrew and remove with hands or screwdrivers the retaining screws pointed by the arrows shown in Figure 8 9 on the right plate of this analyzer Follow the arrow shown in Figure 8 10 to push and remove the right plate Follow the instructions displayed on the screen to place an empty cup below the sample probe Press ENTER to soak the wipe block with the aspirated cleanser The soaking progress will be displayed on the screen as Figure 8 14 See Frey Jens Maintenance Item Diluent prime Lyse test Rinse prime Clean bath Lyse prime Drain bath Zap aperture Drain tubing Flush aperture Clean wipe block Probe cleanser cleaning Prepare to ship E Z cleanser cleaning Cleaning wipe block an wipe block Reagents for gt 99 Counts MENU T 1 lt 7 Select service item ENTER Execute Figure8 14 Cleaning wipe block When the soaking is done wipe the bottom of the wipe block with a fiber cloth that does not leave debris Press ENTER to flush the block and the inner surface of the probe After the flushing is done the screen returns to the initial state When the accumulated analyzed samples reach 4 000 a dialog box will pop up to remind the user to clean the wipe block Figure 8 16 shows You may move the cursor to Yes and press ENTER to start the cleaning
55. Analysis press MENU and a dialog box will pop up as Figure3 4 shows To ignore the entered number move the cursor to No and press ENTER otherwise move the cursor to Yes and press ENTER Whole Blood All 13 45 Current sample WBC 0 0 x 10 L Lymph eee ee OL Mic weet x 10 L W i D p Gran wae ex IDL el Time 01 26 2004 21 14 Lymph ttt i tt T T T T T T Mode Mig 100 200 300 fL Name Gender Age 250 fL Next sample Save changes ID 101 EH Mode WB All PDW ars ree PCT rT 0 5 10 15 20 25 fL LS Figure3 4 Dialog box Note When the ID of the next sample is 0 and you have pushed the START key the system will perform the background check 3 4 2 All Info Mode If you analyzer is configured as All info it means you can enter all pieces of information regarding the sample patient name chart number and the like into this analyzer To enter them press F1 at the Count screen to enter the Enter sample information screen as Figure 3 5 shows Follow the instructions given below to fill out the listed fields one by one 3 8 Auto Hematology Analyzer Operation Manual V1 0 1 Sample Analysis Whole Blood All EEJ Enter sample information Cu D 4 ID 00000119 Gender Time
56. B Use a keyboard to enter the name of the person who sent this sample This edit box automatically saves the entered item to its pull down menu which can be accessed by pressing ENTER Totally 30 items can be saved in the pull down menu and you may press f or to move the cursor to the interested item and press ENTER to select it C When you are done entering the name press T or J to move the cursor to the next field to be edited 9 To enter the name of the person who is to analyze the sample you may A Press f or J to move the cursor the edit box right of Tester B Use a keyboard to enter the name of the person who is to test this sample This edit 3 12 Auto Hematology Analyzer Operation Manual V1 0 Sample Analysis box automatically saves the entered item to its pull down menu which can be accessed by pressing ENTER Totally 30 items can be saved in the pull down menu and you may press fT or to move the cursor to the interested item and press ENTER to select it C When you are done entering the name press T or J to move the cursor to the next field to be edited 10 To enter the name of the person who is to review the analysis result you may A Press f or J to move the cursor the edit box right of Checker B Use a keyboard to enter the name of the person who is to review this sample This edit box automatically saves the entered item to its pull down menu which can be acce
57. BC 2800 Auto Hematology Analyzer Operation Manual Copyright Statement SHENZHEN MINDRAY BIO MEDICAL ELECTRONICS CO LTD hereinafter called Mindray owns all rights to this unpublished work and intends to maintain this work as confidential Mindray may also seek to maintain this work as an unpublished copyright This publication is to be used solely for the purposes of reference operation maintenance or repair of Mindray equipment No part of this can be disseminated for other purposes In the event of inadvertent or deliberate publication Mindray intends to enforce its rights to this work under copyright laws as a published work Those having access to this work may not copy use or disclose the information in this work unless expressly authorized by Mindray to do so All information contained in this publication is believed to be correct Mindray shall not be liable for errors contained herein nor for incidental or consequential damages in connection with the furnishing performance or use of this material This publication may refer to information and protected by copyrights or patents and does not convey any license under the patent rights of Mindray nor the rights of others Mindray does not assume any liability arising out of any infringements of patents or other rights of third parties Content of this manual is subject to changes without prior notice PROPERTY OF SHENZHEN MINDRAY BIO MEDICAL ELECTRONICS CO LTD ALL RIG
58. Date Time Year Month Day JIDIRDIRC 27 Save changes Yes Hour Minute Second a gt lt gt lt gt Reagents for gt 99 Counts MENU 7 2 4 Gain Figure 7 21 Saving changes At the Setup screen press F1 to select the Gain group as Figure 7 22 shows At this screen the gains of WBC WB WBC PB RBC or HGB are visible to all users but changeable only to the administrators Setup Settings Ready 23 44 Coo0e000 Reagent Print amp comm Date amp time Gain Count Ref range Other Digipot RBC HGB WBC WB WBC PB a e Er er Rate 100 0 100 0 100 0 HGB blank V Reagents for gt 99 Counts MENU 7 Move cursor 7 Select setting item F1 Select setting group Figure 7 22Setting gain 7 16 Auto Hematology Analyzer Operation Manual V1 0 Setup 7 2 4 1 Setting WBC gain You may set the WBC gain to adjust the shape of the WBC histograms When the WBC histograms of most samples are similar to the one shown in Figure 7 23 it implies a too small WBC gain that should be increased 0 50 100 150 200 250 300 fL Figure 7 23 Too small WBC gain When the WBC histograms of most samples are similar to the one shown in Figure 7 24 it implies a too large WBC gain tha
59. ENTER to enter the Count screen as Figure 5 2 shows Whole Blood All Current sample WBC 0 0 x 10 L Lymph rete x OIL Mid tee xq W ID 0 Gran ae 10 L a Time 01 26 2004 21 14 Lymph att O Made sei wan 0 100 200 300 fL 0 o HGB 0 g L Name RI RBC 0 00 x 107 L E HCT 0 0 cl Gender MCV bidata y Le i i Re t T T T T T Age MCH pg 0 50 150 260 fL MCHC ae g L Next sample RDW CV me RDW SD fL E ID 1 PLT 0 x 10 L L MPV mt EL Mode WB All me PDW i pe PCT ttt Of 0 5 10 15 20 25 fL Figure 5 2 Count screen 3 Prepare the calibration material as instructed by Section 3 5 and then follow Section 3 6 to analyze the prepared calibration materials in the desired mode for several times at least 5 Auto Hematology Analyzer Operation Manual V1 0 5 3 Calibration times The reproducibility of the WBC RBC HGB MCV and PLT should meet the requirements specified in Table 5 1 5 3 2 Entering the Manual Calibration Screen 1 Press MENU to enter the system menu Press the appropriate arrow keys to move the cursor to Calibration Manual as Figure 5 3 shows Whole Blood All Current sample WBC 9 1 x 10 L Lymph 3 7 x 10 L i Mi cht 0 7 xao Wh ID 118 Grand 47x tol BL Time 01 24 2004 13 55 Lymph H 41 0 i i i Mid 8 0 Mode WB All 0 100 200 300fL cal Gran 51 0 l HGB 124 g L Name RBC 4 08 x 102 L HC L 36 0 Ca
60. ENU 7 Move cursor 1 Select parameter Figure 4 9 L J Graph Screen 1 The 12 parameters are divided into 3 groups for display one group for one screen as Figure 4 9 Figure 4 11 shows You can press f or to switch among the screens At every L J Graph screen you can press or to view the results displayed below the parameter 4 8 Auto Hematology Analyzer Operation Manual V1 0 Quality Control box of every point presented in the graph The current cursor position is displayed in the No field and the time at which this QC analysis was done was displayed in the Time field Controls Ready 14 55 L J Graph File No 1 Lot No 7645 Exp Date 07 27 2004 I Lymph 3 2 2 6 2 6 2 0 Lymph i Gran 1 5 Gran No 1 Time 01 27 2004 Reagents for gt 99 Caunts MENU gt Move cursor 1 Select parameter Figure 4 10 L J Graph Screen 2 Controls Ready 14 55 L J Graph Lot No 7645 Exp Date 07 27 2004 ooonooos L gt ee coo Soe co saan No 1 Time 01 27 2004 Reagents for gt 99 Counts MENU gt Move cursor 1 Select parameter Figure 4 11 L J Graph Screen 3 The L J graph is interpreted as follows 1 The x coordinate represents how many tim
61. Format WBC nee 1049 L Default Lymph NTE 10 3 uL Mid bii 10 2 uL Gran 1a nL Lymph ka Default Mid eit NA Gran ii g L Default HGB Pear g dL me mmol L Auto Hematology Analyzer Operation Manual V1 0 7 19 Setup RBC 10412 L Default 10 6 uL 10 4 uL pL HCT Default L L MCV fL Default um 3 MCH pg Default fmol MCHC g L g dL Default mmol L RDW CV Default RDW SD fL Default um 3 PLT 10 9 L Default 10 3 uL 10 4 uL nL MPV fL Default um 3 PDW NA Default PCT Default mL L Follow the steps given below to select the units Obtain the administrator authority as instructed by Chapter7 1 1 Atthe Setup screen press F1 to select the Count group Press T or J to move the cursor to the edit box right of the desired parameter for instance WBC in the Count field Press ENTER to display the pull down menu 7 20 Auto Hematology Analyzer Operation Manual V1 0 Setup Setup Ready 23 47 Settings Reagent i Group member Print amp comm 0 Lymph Mid Grant Date time Mid Gran Gain Count Ref range Other M MCHC MCH RDVW SD PDW PCT Co
62. GB blank voltage which usually should be set between 4 3 4 5V Follow the steps given below to set the HGB gain Obtain the administrator authority as instructed in Chapter7 1 1 At the Setup screen press F1 to select the Gain group Press fT or J to move the cursor to the edit box right of HGB in the Gain field Press JPgUp or PgDn to enter the desired gain and observe whether the HGB blank voltage is between 4 3 4 5V When you are done entering the gain press T or 1 to move the cursor to other settings you want to change 7 2 4 4 Exiting setting gains When you are done changing all the printing and transmission settings you want to change you may press F1 to select other setting group you want to change or press MENU and a dialog box will pop up to remind you to save the changes as Figure 7 26 shows Press or gt to move the cursor to Yes and press ENTER to save the changes and exit to the system menu or move the cursor to No and press ENTER to exit to the system menu without saving the changes Setup Ready 23 46 Settings Reagent Digipat Print amp comm Rate HGB blank Date dest kOe wec we feo 100 0 Gain Count WBC PB 80 100 0 Ref range T Other s Save changes ves_ i Reagents for gt 99 Counts MENU Figure 7 26 Saving changes 7 18 Auto Hematology Analyzer Operation Manual V1 0 Setup 7 2 5
63. HELP 7 2 2 Printing and Communication Settings You change the printing and communication settings to your own need as Figure 7 9 shows Auto Hematology Analyzer Operation Manual V1 00 7 7 Setup Setup Ready 21 18 Settings Reagent Print Transmit Print amp comm Date amp time Device Bapa Baud 9600 v Gain Format Format 1 Parity None v Count Ref range Auto Off Handshake m oo0oo0o0o00o0 Other Auto X Report title Recorder Printer Reagents for gt 99 Counts MENU gt Move cursor T Select setting item F1 Select setting group Figure 7 9 Printing and Communication Settings 7 2 2 1 Changing Printing Settings 1 Selecting printing device You may choose the internal recorder or an external printer EPSON LQ 300K EPSON LQ 300K or LQ 1600K as the printing device Follow the steps given below to do so At the Setup screen press F1 to select the Print amp comm group as Figure 7 10 shows Press T or 1 to move the cursor to the combo box right of Device in the Print field Press ENTER to display the pull down menu as Figure 7 110 shows Setup Ready 21 19 Settings Reagent Print Transmit Print amp comm Date amp time Device recorder Baud g600 f Gain Format setae at P
64. HH HGB Limit g L HHH PLT Limit 10 L HHHH Lymph Limit 10 L HHEH Lymph Limit H H Gran Limit 10 L HHHH Gran Limit HHH HCT Limit HEH MCV Limit fL HHEH MCH Limit pg HHEH MCHC Limit g L HHHH Body of the text end If handshake is selected EOT If handshake is not selected EOF If handshake is selected ETX If the Lot No Month Day Year are empty in QC Edit menu the 2A Hex will be transmitted to the external computer A 4 4 Run QC Data Format Standard QC Data Format as shown in Table A 4 Table A 4 Run QC Data Format If handshake is selected ENQ If handshake is not selected STX Body of the text start Text Distinction Code C Month HE Day HH A 6 Auto Hematology Analyzer Operation Manual V1 00 Year Hour Minutes WBC 10 L RBC 10 7 L HGBIg L PLT 10 L Lymph 10 L Lymph Gran 10 L Gran HCT MCV fL MCH pg MCHC g L Body of the text end If handshake is selected If handshake is not selected If handshake is selected HHHH HHH HHHH HHH HHEH HHH HHH HHHH HHEH EOT EOF ETX Communication Auto Hematology Analyzer Operation Manual V1 00 A 7 P N 2800 20 28795
65. HTS RESERVED Responsibility on the manufacturer party Mindray is responsible for safety reliability and performance of this equipment only in the condition that e all installation expansion change modification and repair of this equipment are conducted by Mindray qualified personnel applied electrical appliance is in compliance with relevant National Standards Auto Hematology Analyzer Operation Manual V1 00 l AN Note A This equipment is not intended for family usage This equipment must be operated by skilled trained clinical personnel A Warning AY It is important for the hospital or organization that employs this equipment to carry out a reasonable maintenance schedule Neglect of this may result in machine breakdown or injury of human health Warranty THIS WARRANTY IS EXCLUSIVE AND IS IN LIEU OF ALL OTHER WARRANTIES EXPRESSED OR IMPLIED INCLUDING WARRANTIES OF MERCHANT ABILITY OR FITNESS FOR ANY PARTICULAR PURPOSE Exemptions Mindray s obligation or liability under this warranty does not include any transportation or other charges or liability for direct indirect or consequential damages or delay resulting from the improper use or application of the product or the substitution upon it of parts or accessories not approved by Mindray or repaired by anyone other than a Mindray authorized representative This warranty shall not extend to any instrument which has been subjected to misuse negligence or accident
66. RS Sample 3 HGR Sample 4 Save new factors Sample 6 MC Calculate Yes No j ie 0 0 0 0 Reagents for gt 99 Counts MENU Figure5 24 Saving changes To save the new factors press gt to move the cursor to Yes and press ENTER to save the factors except for those out of the calibration ranges to the Manual calibration screen and return to the Auto fresh blood screen in another calibration mode Otherwise move the cursor to No and press ENTER to switch to the Auto fresh blood screen in another calibration mode without saving the new factors Press MENU and a dialog box will pop up to ask you to save the new factors as Figure5 24 shows To save the new factors press lt gt to move the cursor to Yes and press ENTER to save the factors to the Manual calibration screen and return to the system menu Otherwise move the cursor to No and press ENTER to switch to the system menu without saving the new factors 8 Other operations f calibration data calibration results CV or new factors exist when you press F1 a dialog box will pop up to warn you as Figure5 25 shows Press ENTER to return to the Auto fresh blood screen 5 20 Auto Hematology Analyzer Operation Manual V1 00 Calibration Calibration Ready 19 04 Whole Blood Para Mean 1 Factor Predi luted WBC 4 5 43 Sampl
67. Sis 0 ccccccccececeeeeeeeeeeeeeeecaeeeeaaeseeeeeseaeeecaaeeseaaeeseeeeseaeessaaesennees 3 17 3 7 Background Check 3 c ccccesiedl wen bi ite eee il ath hl iaiaeiaeiaa iari 3 25 3 8 SHUTAOWMN eieaa a ae recede a ede aaa aeia n aaa aA eas eo peed 3 26 Chapter 4 Quality Control ccnecs saa a ae dase eas exe ate ewes waste 4 1 4 1 QC with Controls 2 2 00 cece cece cence ecee cece cece eeeeeeaeseeaeeegeeeeceaeeseaaesseaeesneeesaeesteneeea 4 2 4 2 A B Analysis aonuaire ilps cece ive dts Alan gd aaia iad eevee ae eb eee cadens 4 12 Chapter 5 Call Bra tlOnn cs Gos 5 dee ie aan eat eee see ees Sab a ene ee gt 1 51 Introduction 2 0 ei ae eee ea eed Oven hee Ova 5 1 5 2 Preparing for Calibration 0 0 ccccccceeeeeeeceeeeeeeeeeeeeeceaeeeeaaeeeeeeeseeeeesaeeeeaeeseeeeeeeas 5 2 Auto Hematology Analyzer Operation Manual V1 00 l Content 5 3 Manual Calibration 0 5 satan a ae iann tian te 5 3 5 4 Calibration Using Calibrators 00 ccccceccceceeeeeeeeeeeeeee see eeeeaeeeeaeeseeeeeseaeeseneeeeees 5 8 5 5 Calibration Using Fresh Blood cccccceceeeseeceeeee cent eeeeaee scenes seaeeesaeeeeaeeseneees 5 15 Chapter 6 Sample Review niesienie oera eat ey ates Su atic ean ee cay ae tes 6 1 G T Histogram Mode 00 42 3 2 0iec ee HA ee ee hi ee E 6 2 6 2 Review in Table Mode cccececeeeceeeeeeeeeeeeeeeeeeeeeeeeeaeeeeeseeeeeeeeneaeeeeeneaeeeeeeaaeeees 6 5 Chapter 7 StD hate cave
68. W CV RDW SD PLT MPV PDW and PCT plus the RBC and PLT histograms Auto Hematology Analyzer Operation Manual V1 0 3 17 Sample Analysis Biohazard Consider all materials specimens reagents controls calibrators or components that contain or have contacted human blood as being Bp potentially infectious Wear standard laboratory attire glove and follow safe laboratory procedures when handling any material in the laboratory Warning The probe is sharp and may contain biohazard materials including controls and calibrators Avoid any unnecessary contact with the probe and probe area Note To have an accurate aspiration be sure to keep certain distance between gt the probe and the bottle bottom during the aspirating process 3 6 1 2 Analyzing Whole Blood Samples Follow the steps given below to analyze a whole blood sample 1 Press MODE to select the appropriate analysis mode 2 Present the whole blood sample to the sample probe Press START and the probe will automatically aspirate 131 sample 3 Remove the sample when aspiration is done when the analyzer buzzes and the sample probe has risen up 4 Wait for the analyzer to finish the analyzing process during which the center of the screen displays the analyzing progress and the System Status Area displays Running When the analysis is done the result is displayed in the Analysis Result Area and the ID in the Next sample area automatic
69. ally increases by one 3 6 1 3 Analyzing Prediluted Blood Samples Follow the steps given below to analyze a prediluted blood sample 1 Press MODE to select the appropriate analysis mode 2 Present the prediluted blood sample to the sample probe Press START and the probe will automatically aspirate 0 7ml sample 3 Remove the sample when aspiration is done when the analyzer buzzes and the sample probe has risen up 4 Wait for the analyzer to finish the analyzing process during which the center of the screen displays the analyzing progress and the System Status area displays Running When the 3 18 Auto Hematology Analyzer Operation Manual V1 0 Sample Analysis analysis is done the result is displayed in the Analysis Result area and the ID in the Next Sample Part automatically increases by one 3 6 1 4 Printing Analysis Results You can print out the displayed analysis result either automatically or manually If you want to print out the analysis result automatically refer to section 7 2 2 Printing and Transmission to set the Autoprint to On and select the built in recorder or the external printer as the printing device The analysis result will be automatically printed out to the selected device when the analysis is done If you want to print out the analysis results manually set the Autoprint to Off and press PRINT at the Count screen to print out the displayed analysis result to the selected printing device Note
70. also marked the corresponding error codes that are explained in Chapter 10 1 the Times column displays how many times 1 255 this event occurred and if it occurred more than 255 times the excessive events will be recorded from 1 to another log file the Information column displays extra information regarding the event This analyzer can save maximum 100 log files and once the maximum number has been reached the newest log will automatically cover the oldest one Auto Hematology Analyzer Operation Manual V1 0 8 23 Maintenance 8 6 System Configuration To view the system configuration press MENU to enter the system menu and then press the appropriate arrow keys to move the cursor to Service gt nfi g Press ENTER to enter the Config screen as Figure 8 26 shows where you can only view the system configuration without changing it Service Ready 00 35 Item Result Software version S N Storage capacity Discriminator Enter sample info Search Expiration date Clean condition PS 2 keyboard Edit sample info Printer Transmission Barcode 1 0 0 AC 000000 10000 Displayed All info Reagents for gt 99 Counts MENU T 1 Scroll a line PgUp PgDn Scroll a page Figure 8 26 Configurat ion Every screen displays 13 items and you can press T or J to select the item you want to see or press PgUp or PgDn to go to the previous or next scr
71. alyzer Operation Manual V1 00 Sample Review 6 2 Review in Table Mode At the Review screen and in the Histogram mode and press to enter the Table mode as Figure6 5 shows To review the sample results saved in either the Sample or Searched database you also must first set the desired database as the Database by pressing T Review Ready 20 39 Reagents for gt 99 Counts MENU T Searched Histogram F1 Goto F2 Select F3 Transmit F4 Search Figure 6 5 Sample table The results saved in the chosen database are displayed on the screen 8 results for one screen sequentially from Location 1 You can press PgUp or PgDn to switch among the screens If you want to acquire help information press HELP If you want to exit press MENU to enter the system menu and access the desired screen from there 6 2 1 Reviewing Result of a Specific Sample In the Table mode you can also follow the steps introduced in Chapter 6 1 1 to review the result of a specific sample The selected sample is marked above its ID as Figure 6 6 shows the sample ID 117 is selected Auto Hematology Analyzer Operation Manual V1 0 6 5 Sample Review Review Ready 20 40 Reagents for gt 99 Counts MENU 1 Searched Histogram F1 Goto F2 Select F3 Transmit F4 Search Figure 6 6 Select a sample 6 2 2 Selecting Re
72. and follow safe laboratory procedures when handling any material in the laboratory Cautions Avoid direct contact with blood specimens 3 5 1 Preparing Whole Blood Samples Mindray recommends BC 2800 be used to analyze the whole blood samples that use K EDTA as the anticoagulant The demanded dose of the anticoagulant is 1 5 2 2mg ml blood 3 5 2 Preparing Prediluted Samples 1 Press MODE to select the prediluted mode any mode preceded by PB 2 Press DILUENT to enter the Add Diluent screen as Figure 3 9 shows Prediluted WBC HGB Current sample WBC 0 0 x 10 L i Lyrmphi see TOL i Mi ci ers x 1997L WI ID 0 Grand me xo BN Time 01 26 2004 21 14 Lymph we OG f i wk OF Li T T T T T T Mode mice O 100 200 300 fL Add Diluent i Name Present the sample tube to the probe Gender Press START to add diluent Press EID Oe ere eed Age ENTER to exit 120 220 fL Next sample RDVW SD eee t EL ID 1 PLT 0 x 10 L MPV ae t fL H Mode PB WBC HGB i PDW ane a Ea net ste og 0 5 10 15 20 25 fL RN Figure 3 9 Add Diluent screen Auto Hematology Analyzer Operation Manual V1 0 3 15 Sample Analysis 3 Present a clean sample cup to the sample probe and press START to add 1 6ml diluent controlled by the analyzer itself to the cup Be sure to incline the cup at the angle shown in Figure 3 10 to force the diluent to flow into the cup against the cup wa
73. anser cleaning procedure If you want to do so move the cursor to Yes and press ENTER Otherwise move the cursor to No and press ENTER 8 1 8 Lyse Test Biohazard Wear standard laboratory attire glove and follow safe laboratory procedures when handling any material in the laboratory In case of any abnormal WBC counts or histograms you can perform the Lyse test procedure to check whether the lyse can be dispensed properly Follow the steps given below to do so Unscrew and remove with hands or screwdrivers the retaining screws pointed by the arrows shown in Figure 8 9 on the right plate Auto Hematology Analyzer Operation Manual V1 0 8 9 Maintenance SIONI ORAI z A Figure 8 9 Removing the two screws Follow the arrow shown in Figure 8 11 to push and remove the right plate Figure 8 10 Removing right plate Remove the screws fixing the shielding box of the bath as Figure 8 11 shows H I Figure 8 11 Shielding box Remove the shielding box to expose the bath as Figure 8 12 shows 8 10 Auto Hematology Analyzer Operation Manual V1 00 Maintenance Figure 8 12 Bath At the Maintenance screen press the appropriate arrow keys T gt to move the cursor to Lyse test Press ENTER and this analyzer will automatically drain the bath and then dispense 2ml lyse to the bath Check the scale to see whether the lyse has reached the expected line th
74. ar last calibration data Sample 6 MC Calculate Yes no ie 0 ie 0 O Reagents for gt 99 Counts MENU Figure5 27 Warning dialog box To clear the previous results press or gt to move the cursor to Yes and pres ENTER to clear the previous results and enter the Auto fresh blood screen Otherwise move the cursor to No and press ENTER to save the previous results and enter the Auto fresh blood screen At the Auto calibration screen when the CV and new factors are available you can press PRINT to print out the displayed information To acquire help information press HELP To return to the system menu press MENU 5 5 4 Testing New Factors After you have obtained the new factors choose one of the following two methods to test the new factors Method one Prepare 3 5 fresh blood samples and test them on a reference analyzer for at least three times and take the averages and SD as the reference values Test these samples on this analyzer for at least three times and take the averages Compare the averages to the reference values and the difference should be within 2SD Method two After the new factors are saved go to the Count screen to analyze the calibrator or control material for at least five continuous times Take the average of the analysis results and compare it to the expected value and make sure the average is within the expec
75. ard the result If the obtained result is invalid a dialog will pop up to warn you as Figure 5 12 shows Press ENTER to close the dialog box and discard the result 5 10 Auto Hematology Analyzer Operation Manual V1 0 Calibration Calibration Auto Ready 16 02 Invalid result Whole Blood O Prediluted Reagents for gt 99 Counts MENU Figure 5 12 The warning dialog box 4 After you have saved three calibration results this analyzer will automatically display the CV and calibration factors You can save maximum five calibration results as Figure5 13 shows Note that the CVs should be within the ranges specified in Table 5 1 Calibration Lot No 62 Exp Date Ready 16 05 06 10 2004 Whole Blood O Prediluted Para Mean 1 2 3 4 5 CV Factor WBC 6 0 RBC HGB MCV PLT 0 1 3 3 0 0 165 62 529 4 Reagents for gt 99 Counts MENU F1 Select mode F2 Edit reference Figure5 13 Results of auto calibration Any factor that falls out of the calibration range will be flagged by a at the upper right corner When it happens you should try to find out the reason If the problem is beyond your ability contact Mindray customer service department Auto Hematology Analyzer Operation Manual V1 0 5 11 Calibration 5 Exiting To sa
76. arity None v Count i None Ref range Auto Handshake No oo0oo0o0o00o0 Other F Auto Off X Report title Recorder Printer Reagents for gt 99 Counts MENU gt Move cursor 1 Select setting item F1 Select setting group Figure 7 10 Selecting printing device Press T or J to move the cursor to Recorder or Printer and press ENTER to confirm the selection When you are done selecting the printing device press 1 or J to move the cursor to other printing settings you want to change 7 8 Auto Hematology Analyzer Operation Manual V1 00 2 Setting printing format Setup The printing format determines the format in which the printed contents appear in the printout Follow the steps given below to select the printing format Atthe Setup screen press F1 to select the Print amp comm group Press T or J to move the cursor to the edit box right of Format in the Print field Press ENTER to display the pull down menu as Figure 7 11 shows Setup Settings Ready Reagent Print amp comm Date amp time Gain Count Ref range Other Device Farmat Auta Report title Recorder Printer Print Recorder X Format 1 X Format 1 6 Format 2 Format 3 Format 4
77. as Figure 7 29 shows Press or gt to move the cursor to Yes and press ENTER to save the changes and exit to the system menu or move the cursor to No and press ENTER to exit to the system menu without saving the changes Auto Hematology Analyzer Operation Manual V1 0 7 21 Setup Setup Settings Ready 23 48 Reagent Print amp comm Date amp time Gain Count Ref range Other Group member Save changes Lymph Mid Gran Mid Gran PDW PCT 7 Count t WBC Second RBC Second Reagents for gt 99 Counts MENU 7 2 6 Reference Ran Figure 7 29 Saving changes ge You can set a reference range for every parameter The system will flag any analysis result that exceeds this range with either an H or L This analyzer divides the patients into 5 patient groups which are all listed in Table 7 4 Table 7 4 Patient groups Patient Goup Sex Age Nt specified Nt specified General Not specified gt 13 years Mle adults Mn gt 13 years Fenal e adul ts Vonan gt 13 years Pedi atric a f Nt specified gt 1 nonth and 13 years pati ents Neonat es Nt speci fied lt nonth The upper and lower limits of the reference ranges are visible to all users but changeabl
78. ath Zap aperture Drain tubing Flush aperture Clean wipe block Probe cleanser cleaning Prepare to ship E Z cleanser cleaning Prime the bath with diluent Press ENTER to continue 00 00 22 Reagents for gt 99 Counts MENU T 1 lt 7 Select service item ENTER Execute Figure 8 13 Drain Bath 8 1 11 Draining Tubing If this analyzer is not to be used for a long time or it is to be maintained be sure to perform the Drain tubing procedure Follow the steps given below to do so Atthe Maintenance screen press the appropriate arrow keys T 4 to move the cursor to Drain tubing Press ENTER and follow the displayed instructions to remove all the tubes except for the one for the waste from this analyzer Press ENTER to start the draining procedure When the draining is done the screen will display Turn off this analyzer and you should turn off this analyzer as instructed 8 1 12 Cleaning Probe Wipe Block After being used for a long time the bottom of the probe wipe block may be contaminated by blood and the inside of the block may also be contaminated by the dirt sucked in So you need to clean the wipe block regularly Follow the steps given below to do so Atthe Maintenance screen press the appropriate arrow keys T J to move the cursor to Clean wipe block Present the probe cleanser to the sample probe and press ENTER to aspirate the
79. bility and precision of the analysis results be sure to shut down the analyzer by the Shutdown procedure after it runs continuously for 24 hours Follow the steps given below to perform the Shutdown procedure 1 Press MENU to enter the system menu and press ft or to move the cursor to Shutdown as Figure 3 16 shows Whole Blood All Current sample WBC Lymph Mid ID 118 Grang Time 01 24 2004 13 55 Lymph Mode WB All Mid Gran HGB Name RBC e a aa Count H Review LHC DW CV Quality control gt IWw 5D Setup gt T Service gt ly Calibration gt Ww T Shutdown E x 10 L x 10 L x 10 L x 10 L A 0 b Owo CoOOs4H HS n 124 g L 4 08 x 102 L 36 0 88 3 fL 30 3 pg 344 g L 13 6 40 2 fL 222 x 10 L 9 0 fL 15 7 0 199 50 250 fL 300 fL Figure 3 16 Performing the Shutdown procedure from the system menu 2 Press ENTER to enter the Shutdown dialog box as Figure 3 17 shows Shutdown Figure 3 17 Shutdown dialog box gt 99 Counts 3 26 Auto Hematology Analyzer Operation Manual V1 00 Sample Analysis 3 To Shutdown this analyzer move the cursor to Yes and press ENTER to enter the Shutdown screen as Figure 3 18 otherwise move the cursor to No and press ENTER to exit the dialog box Shutdown Shutdown Press START to aspirate 1 6ml E Z cleanser Turn off the
80. criminators manually if the WBC result is less than 0 5 or out of the operation range The first three discriminators of the WBC histogram are adjustable so are the two of the RBC histogram and the two of the PLT histogram Assuming you want to shift the third discriminator of the WBC histogram to 100fL follow the steps given below to do so 1 Atthe Count screen press ENTER to activate the discriminators as Figure3 12 shows Whole Blood Alll uag a Current sample WBC 8 1 x 10 L rak g i Lymph 3 7 x 10 L i Mick 07x 10 Wy ID 118 Gran 47x1 Bj Time 01 24 2004 13 55 Lymph H 41 0 Mid 8 0 WB All f Mode B Gran 510 0 100 a 300 fL HGB 24 g L Name RBC 4 08 x 1017 L HCT L 36 0 Gender MCV 88 3 fL i i Age MCH 30 3 pg 0 50 150 260 fL MCHC 344 g L Next sample RDW CV 13 6 i RDW SD 40 2 fL ID 119 PLT 222 x 10 L T MPY 9 0 fL f Mode WB All PDW 157 l PCT 0 199 Oo 6 10 15 20 25 fL feagents for gt 99 Counts MENU 1 1 Histogram F1 F2 F3 Discriminator Shift ENTER Exit Figure3 12 Activating discriminators 3 22 Auto Hematology Analyzer Operation Manual V1 0 Sample Analysis 2 Press f or J to select the WBC histogram 3 Press F3 to select the third discriminator as Figure3 13 shows Whole Blood Alll 2 g 5 Current sample WBC 9 1 x 10 L ai d Lymph 3 7 X 10 L i Mi ci 07x 10 7L Wy iD 2118 Grant 47x
81. d perform the Lyse prime procedure to prime the lyse tubing with lyse when there are bubbles in the tubing or the lyse in the tubing is contaminated or the old lyse ran out and a new container of lyse is installed Follow the steps given below to do so At the Maintenance screen press the appropriate arrow keys T 4 gt to move Auto Hematology Analyzer Operation Manual V1 0 8 5 Maintenance the cursor to Lyse prime Press ENTER to prime the tubing with lyse and the priming progress will be displayed at the bottom of the screen as Figure 8 5 shows When the priming is done the screen will return to the initial state Service Ready 23 59 Maintenance Item Diluent prime Lyse test Rinse prime Clean bath prime Drain bath Zap aperture Drain tubing Flush aperture Clean wipe block Probe cleanser cleaning Prepare to ship E Z cleanser cleaning Priming lyse Ly se prime CRRCCCCCCCCCCCCCCCCCCENNE 24 Reagents for gt 99 Counts MENU T 1 lt 7 Select service item ENTER Execute Figure 8 5 Priming tubing with lyse 8 1 4 Zap Aperture You can perform the Zap aperture procedure to unclog or prevent clogging Follow the steps given below to do so At the Maintenance screen press the appropriate arrow keys T gt to move the cursor to Zap aperture Press ENTER to zap the aperture and the zapping progress wil
82. days When you are done handling the control material treat and analyze it the same as patient samples Refer to section 2 6 Sample Analysis for detailed operation procedures Caution Mindray demands only the recommended controls be used on the BC 2800 and will not take responsibility for inaccurate results if other controls has bee used on it Auto Hematology Analyzer Operation Manual V1 00 4 7 Quality Control Biohazard Consider all materials specimens reagents controls calibrators or components that contain or have contacted human blood as being potentially infectious Wear standard laboratory attire glove and follow safe laboratory procedures when handling any material in the laboratory Warning The probe is sharp and may contain biohazard materials including controls and calibrators Avoid any unnecessary contact with the probe and probe area 4 1 5 Reviewing the QC Results You can review the saved results in either of the two modes L J Graph and Table 4 1 5 1 L J Graph At the Controls screen press F4 to enter the L J Graph screen as Figure 4 9 shows Controls Ready 14 54 L J Graph File No 1 Lot No 7645 Exp Date 07 27 2004 I WBC 49 Mean 3 3 45 4 5 H Diff 2 25 3 00 es Hian 2 80 Diff go Sam CRE 86 H Diff 42 50 93 75 57 Saar No 1 Time 01 27 2004 Reagents for gt 99 Counts M
83. department 5 Press F1 to select at least another two fresh samples from Sample 2 Sample 5 and analyze them as instructed above 6 When the analyses are done press F1 to select Calculate to enter the Calculate screen as Figure5 23 shows The number 1 5 represents the new factors corresponding to the fresh blood samples 1 5 Calibration Ready 19 00 Whole Blood Predi luted Para 1 Factor AVG Sample 1 Sample 2 Sample 3 Sample 4 Sample 5 Calculate o000O0O ooe WBC RBC HGB MCV PLT 104 7 Reagents for gt 99 Counts MENU F1 Select mode F3 Select sample Figure5 23 Calculate Auto Hematology Analyzer Operation Manual V1 00 5 19 Calibration This screen can maximum display the calibration factors for 5 fresh blood samples Any factor out of the calibration range will be flagged by at the upper right corner For any parameter such as the RBC in the new calibration factor will be obtained only when at least three calibrations are valid otherwise the new factor will be blank as the WBC in Figure5 23 7 Exiting Press F3 and a dialog box will pop up to ask you to save the new factors as Figure5 24 shows Calibration Ready 19 20 Auto fresh blood Whole Blood para 1 2 3 4 5 Factor AVG Predi luted WBC 104 7 107 1 104 7 105 5 Sample 1 Sample 2
84. der Out of Paper Possible causes recording paper has run out or is not correctly installed Auto Hematology Analyzer Operation Manual V1 0 9 7 Troubleshooting Solution Check whether the recording paper has run out If so load new paper if not re install the existing paper as instructed by Chapter 2 3 3 If the error still remains call Mindray Customer Service Department or the distributor 9 2 17 Recorder Communication Error Call Mindray Customer Service Department or the distributor 9 2 18 Recorder Too Hot Possible causes the recording head overheats Stop using the recorder If the error repeats call Mindray Customer Service Department or the distributor 9 2 19 Press Bar Up The press bar of the recorder is up Push it back as instructed by Chapter2 3 3 If the error still remains call Mindray Customer Service Department or the distributor 9 2 20 Rotation Motor Error Something is wrong with the motor that rotates the sample probe Access Service gt Self test gt Machine and test the motor as instructed by Chapter 8 4 2 The error will be cleared if the result is normal Otherwise call Mindray Customer Service Department or the distributor 9 2 21 Lyse Expired The lyse has expired Check whether the lyse has expired If so change a new container of lyse as instructed by Chapter 2 3 if not access Setup gt Settings gt Reagents and adjust the expiration date as instructed by Chapter 7 2
85. e 1 Sample 2 RS Sample 3 HG Sample 4 Results present Cannot edit Sample 6 MC Calculate ie 0 ie 2 0 e Reagents for gt 99 Counts MENU Figure5 25 Reject editing dialog box If the valid results are less than three the CV and new factors are not available yet If you press F3 a dialog box will pop up to warn you about the data loss as Figure5 26 Calibration Ready 19 05 Auto fresh blood Whole Blood Para Mean 1 2 3 Factor Predi luted WBC 4 5 43 Sample 1 Sample 2 RS Sample 3 HGR Sample 4 Changing mode will lose data Sample 6 MC Calculate Yes no OOOO OO Ce Reagents for gt 99 Counts MENU Figure5 26 Warning dialog box To switch the modes press or gt to move the cursor to Yes and press ENTER and the saved data will be cleared Otherwise move the cursor to No and press ENTER to exit lf you press MENU the system menu will pop up When you enter the Auto fresh blood screen again a dialog box will pop up as Figure5 27 shows Auto Hematology Analyzer Operation Manual V1 0 5 21 Calibration Calibration Ready 19 11 Auto fresh blood Whole Blood para Mean 1 2 3 4 5 Factor Predi luted WBC 45 43 45 43 3 29 104 7 Sample 1 Sample 2 PB Sample 3 HGR Sample 4 Cle
86. e Search screen and start the search you may Press T or J to move the cursor to Yes and press ENTER to start the search Or Press T or J to move the cursor to No and press ENTER to abort the search and exit to the exit to the Review screen When the search is done a dialog box will pop up to display the result of the search as Figure 20 shows After viewing the dialog box press ENTER to exit The searched results are saved to the Searched database which can only store no more than 500 results of the latest search 6 14 Auto Hematology Analyzer Operation Manual V1 0 Sample Review Review Ready 20 56 Found 500 sample results See result in Searched Database Reagents for gt 99 Counts MENU Figure 6 20 Result of the search You cannot delete the results saved in the Searched database If new sample results are added to the Sample database or the sample results saved in the Sample database are changed the Searched database will be automatically emptied Restarting this analyzer will also empty the Searched database 6 2 6 Printing If you want to print out the selected sample results press PRINT 6 2 7 Special Functions To access the Special Functions screen you must first select several 1 500 sample resul
87. e for the rinse 4 When you are done entering the date press T or J to select the other reagent settings to be changed 7 2 1 4 Exiting editing reagent settings When you are done change all the reagent settings you want to change you may press F1 to select other setting group you want to change or press MENU and a dialog box will pop up to remind you to save the changes as Figure 7 8 shows Press or gt to move the cursor to Yes and press ENTER to save the changes and exit to the system menu or move the cursor to No and press ENTER to exit to the system menu without saving the changes Setup Waiting 21 18 Settings Reagent Remains Remains Print amp comm Date amp time Diluent 20 00 Gain Waste Rinse 05 50 Count Container Ref range Lyse 500 0 Other Save changes Cres Ea Lyse 1 EH E 2005 K Rinse out Reagents for 0 Counts MENU Figure 7 8 Saving changes Note that if any entered value is beyond the valid range a dialog box will pop up when you exiting to the system menu Press ENTER to close the dialog box and clear the invalid values 7 2 1 5 Other operations 2 If you did not change any of the reagent settings you may press F1 to select other setting group or press MENU to directly exit to the system menu 3 Ifyou want to acquire help information press
88. e item ENTER Execute Figure 8 7 Flushing aperture Auto Hematology Analyzer Operation Manual V1 0 8 7 Maintenance 8 1 6 Probe Cleanser Cleaning Biohazard Wear standard laboratory attire glove and follow safe laboratory procedures when handling any material in the laboratory You can soak the bath and tubing with the probe cleanser an alkaline detergent by performing the Probe cleanser cleaning procedure If your analyzer is to run 24 hours a day you should perform this procedure every 3 days If you follow the shutdown procedure to turn off your analyzer everyday you should perform this procedure every week Follow the steps given below to do so At the Maintenance screen press the appropriate arrow keys T gt to move the cursor to Probe cleanser cleaning Present the cleanser to the probe and press ENTER to aspirate the cleanser Remove the cleanser after the probe has risen up This analyzer will automatically soak the bath and tubing with the aspirated cleanser The soaking process will last about 5 minutes and you may press ENTER to stop the process any time Note that a shortened soaking process may not bring such a good result as a complete one When the soaking is done press ENTER to flush the bath and tubing The screen will return to the initial state To make sure this analyzer functions normally every time the accumulated analyzed samples reach 200 a dialog box
89. e only to administrators as Figure 7 30 shows Follow the instructions below to set the limits Auto Hematology Analyzer Operation Manual V1 0 Setup Setup Ready 23 48 Settings Reagent Para Lower Upper Para Lower Upper Print amp comm wB 40 10 0 MCV 82 0 95 0 ate Stime Lymph 0 8 0 MCH 7 0 31 0 sis Mid 0 1 MCHC Ref range Grant 20 0 RDW CV Other Lymph 20 0 RDW SD Mid 3 0 0 PLT Gran 50 0 0 MPV HGB 110 0 POW RBC 3 50 5 50 PCT 37 0 F2 Save F4 Default Reagents for gt 99 Counts MENU 7 Move cursor 1 Select setting item F1 Select setting group Figure 7 30 Setting reference range 7 2 6 1 Selecting patient group Follow the steps given below to select the patient group you want Atthe Setup screen press F1 to select the Ref range group Press T or J to move the cursor to the combo box right of Group Press ENTER to display the pull down menu as Figure 7 31 shows Setup Ready 23 49 Settings Reagent 4 0 Print amp comm WBC oe amp time iymph 0 8 Count Mid 0 1 Ref range Gran Other Lymph Mid Gran HGB RBC HCT Group Reagents for gt 99 Counts MENU 7 Move cursor 1 Select setting item F1 Select setting group Figure 7 31 Selecting patient group Auto Hematology Analyzer Operation Manual V1 00 7 23 Setup Press T or 1 to move the curs
90. e second from bottom In case the dispensed lyse has failed to reach this line for several consecutive times check whether the lyse has run out or the lyse tubing is not properly connected to this analyzer If the lyse is still enough and the tubing is well connected to the analyzer contact the Mindray customer service department for repair Press ENTER to flush the bath and finish the test The screen will return to the initial state 8 1 9 Clean Bath When you keep finding abnormal results from the background check follow the steps given below to do so At the Maintenance screen press the appropriate arrow keys T to move the cursor to Clean bath Press ENTER to start the procedure When the cleaning is done the screen returns to the initial state 8 1 10 Drain Bath When you need to drain the bath follow the steps given below to perform the Drain bath procedure At the Maintenance screen press the appropriate arrow keys T to move the cursor to Drain bath Press ENTER to start the procedure Auto Hematology Analyzer Operation Manual V1 0 8 11 Maintenance When the bath is drained the screen displays Prime the bath with diluent as Figure 8 13 shows Press ENTER to continue and when the priming is done the screen returns to the initial state Service Ready 00 03 Maintenance Item Diluent prime Lyse test Rinse prime Clean bath Lyse prime Drain b
91. e senate eeaeeeeaaeseeeeeseaeeesaeeseaeeseeeeess 1 6 1 50 SSpecicationS i 4t 25 cin cae nial ehh nie each eee aie 1 11 1 6 System Operation cii 3 c ccveieiel A ieee ai eee aad ia a adai qed 1 15 1 7 Operation SUMMALY cece cee e cence cece neta ceeeeeaeeseeeee cae eeeaaeseeaeeseeeesnaeeseaeeneaes 1 22 Chapter 2 installati n 32 8 0 sat he owen cranes bara gon aan ava we e EENE 2 1 ZL UMPACKING sss eesti eict ecg alesis eed eed eat aed er eed 2 1 2 2 Installation Requirement 0 0 cece eeeeeee ee eeee eee eeeeeeeeeeaeeeeesaeeeeeeeeaeeetneaaees 2 2 2 3 Installation reao eara Ai ea iis Se es ea eee Dan ee dee ee ls 2 3 2 4 Loading Recording Paper ccccccceceeeeeeeeeeeeeneeeeeeeeeeeeeeeeeeeeeeeeeeeseneaeeeeeeeaees 2 6 2 5 Installing PriNt tn anina aoaia adaa e a aa a a a arae 2 8 2 6 Installing Scanner optional 0 cccccecceeeeeeeeeeeeeeeceeeee sae eeeeaeeeeeeeseaeeesaeeeeneeees 2 9 Chapter 3 Sample Analysis 2 cc eee ee eee ence nees 31 3 1 Preparing to Start oein ela aie Ak aa is AN ene ee 3 1 3 2 sROWCR UP viisies cit octet des einen te ete cde ses ee ee ee 3 3 3 3 Entering Count Screen 0 ccccccccce cece eeeeeceneeeeeaeeeeeeeeceaeeeeaaeseeaeeseeeeesaeeeeaeseeeeeseas 3 4 3 4 Entering Sample Information 0 00ceccccece cece eeeneeeeeeee eae eeeeaeeeeeeeseaeeeseaeeeeneeees 3 7 3 5 Preparing Samples ceni iienaa aiie a a iaa diya a 3 15 3 6 Sample Analy
92. e the cursor within the edit box Press PgUp or PgDn or the numeric keys of a keyboard to enter a digit at the position where the cursor is located To modify an entered digit you can move the cursor to the corresponding location and re enter the desired digit Note that you can only enter a positive integer that is no greater than 31 in this edit box When you are done entering the patient age press f or to move the cursor to the next field to be edited To enter the chart number of the patient you may A B Press f or to move the cursor to the edit box right of Chart No Press lt or to move the cursor within the edit box Press PgUp or PgDn or the numeric keys of a keyboard to enter a digit at the position where the cursor is located If you want to modify an entered digit just move the cursor to that position and enter the desired digit When you are done entering the chart number press f or to move the cursor to the next field to be edited To enter the bed number of the patient you may A B Press ft or to move the cursor to the edit box right of Bed No Press lt or to move the cursor within the edit box Press PgUp or PgDn or the numeric keys of a keyboard to enter a digit at the position where the cursor is located If you want to modify an entered digit just move the cursor to that position and enter the desired digit When you are done enter
93. e the reagents be sure to read the tags on the package and the supplied data sheet 1 3 5 1 Reagents 1 Diluent Besides diluting the blood sample the diluent provides an environment similar to blood plasma to keep the sizes of blood cells unchanged within a certain period of time and serves as a conductive media for blood cells analysis 2 Lyse The lyse can rapidly break down the red blood cell membranes and reduce cellular debris to a size small enough so that it does not interfere with white blood cell analysis White cells can then be segregated into three sub populations for differential analysis The absorbency of the lyse hemoglobin mixture is determined by the hemoglobin 3 Rinse The rinse is a detergent used to wash the tubing and wet the volumetric tube 4 E Z Cleanser The Enzymatic Cleanser is an enzyme based isotonic cleaning solution and wetting agent used to rinse the tubing and bath This cleanser will not damage the plastic parts of the analyzer 5 Probe Cleanser The probe cleanser is used to maintain the analyzer 1 3 5 2 Calibrators and Controls Calibrators and controls are whole blood products manufactured to calibrate and verify the operation of instruments The calibrators and controls that appear in the rest of this manual refer to the calibrators and controls to be used on this analyze only You shall order them only from Mindray customer service department or the distributor Auto H
94. ecorder Gain Format Format 1 Count Ref range Auto Off Other COoO000800 Report title Recorder Printer Reagents for gt 99 Counts MENU gt Move cursor f Select setting item F1 Select setting group Figure 7 14 Selecting Baud rate Press T or 1 to move the cursor to 9600 and press ENTER to confirm the selection When you are done selecting the printing device press fT or 1 to move the cursor to other printing settings you want to change 2 Setting parity You choose to check the odd bits even bits or no check default setting Follow the steps given below to do so Atthe Setup screen press F1 to select the Print amp comm group Press T or J to move the cursor to the combo box right of Parity in the Transmit field Press ENTER to display the pull down menu as Figure 7 15shows Setup Ready 23 41 Settings Reagent Print Transmit Print amp comm Date amp time Device Recorder Baud 9600 Gain Format Format 1 Parity one Count Ref range Auto Off Handshake COo00080 Other Auto Report title Recorder Printer Reagents for gt 99 Counts MENU lt 7 Move cursor T Select
95. een If you want to print out the configuration press PRINT If you want to acquire help press HELP 8 24 Auto Hematology Analyzer Operation Manual V1 00 8 7 Printing Management Maintenance Press the MENU to enter the system menu and press the appropriate arrow keys T JL to move the cursor to Service Print as Figure 8 27 shows Service Config Item Result 1 Software version 2 S N 3 Storage capacity 4 Discriminator 5 6 Enter sample info Search 1 0 0 AC 000000 10000 Displayed All info Reagents for gt 99 Counts Count Review Maintenance Quality control gt Status Setup gt Self test Log Calibration gt Config Shutdown MENU T 1 lt 7 Select sub menu ENTER Enter sub menu Figure 8 27 Entering print screen Press ENTER to enter the Print screen as Figure 8 28 shows The printing tasks are queued in this screen where you can view the all tasks and delete those waiting to be processed Once something goes wrong with the printing device the task being processed will be deleted and the queued tasks will keep waiting Once the system finds the error has been removed it will resume printing and process the tasks from the first one Note that you cannot change the sequence of the queued tasks Service Print Ready 00 35 Reagents for gt 99 Count
96. ematology Analyzer Operation Manual V1 0 1 5 Introduction 1 4 Principles of Operation 1 4 1 Measuring Principle This analyzer adopts the Coulter Principle to count WBC RBC and PLT cells and to draw their corresponding histograms The Hemoglobin concentration HGB is obtained by the colorimetric method The result of the rest parameters are derived from those see Chapter 1 4 4 Derivation of Parameters 1 4 2 Dilution Usually in blood specimens the cells are too close to each other to be identified or measured For this reason the diluent is used to separate the cells so that they are drawn through the aperture one at a time as well as to create a conductive environment for blood analysis This analyzer can processes two types of blood samples whole blood samples see Chapter 2 5 1 Preparing Whole Blood Samples and prediluted blood samples see Chapter 2 5 2 Preparing Prediluted Samples When analyzing a whole blood sample this analyzer aspirates 13 L of the sample and follow the procedure presented in Figure 1 1 to dilute it before proceeding to the actual analysis Whole Blood Sample 13uL 1 231 Dilution Diluent O 3mL Diluent 3 5mL For RBC Analysis 1 44862 Dilution For WBC Analysis 1 308 Dilution Figure 1 1 How a whole blood sample is diluted 1 6 Auto Hematology Analyzer Operation Manual V1 00 Introduction When analyzing a prediluted sample the operator should
97. ents controls calibrators or components that contain or have contacted human blood as being potentially infectious Wear standard laboratory attire glove and follow safe laboratory procedures when handling any material in the laboratory WARNING Handle and dispose of the waste according to acceptable laboratory local state and national standards 3 1 3 Checking Tubing and Power Supply Check the diluent rinse lyse and waste tubing and make sure they have no bends and are well connected to this analyzer Check the power plug of BC 2800 and make sure it is well plugged into a power socket 3 1 4 Checking Recorder and Printer optional Check and make sure there is enough paper installed on the built in recorder and or the external printer if connected Check and make sure the printer s power plug is well plugged into a power socket and is properly connected to BC 2800 3 1 5 Checking Keyboard optional You need a keyboard to enter complicated information such as patient names department names and the like Make sure the keyboard is well connected to the keyboard interface marked KB on the left side of BC 2800 3 2 Auto Hematology Analyzer Operation Manual V1 0 Sample Analysis Turn on BC 2800 by pressing the power switch on the back and the power indicator will go It takes about 80 seconds for this analyzer complete the initialization process during which The screen displays the initialization picture a
98. er 01 28 200 01 28 200 01 28 200 01 27 200 01 27 200 01 27 200 01 27 200 01 27 200 01 27 200 4 4 4 4 4 4 4 4 4 4 01 27 200 Error 0405 occurred 1 Self tes Error 4002 occurred 1 Self tes Error 400 Ref rang Self test Error 400 Self test 2 occurred 2 occurred Error 400 2 occurred 1 Reagents for gt 99 Counts MENU F1 Select log type f 1 PgUp PgDn Scroll Figure 8 25 Log screen The recorded events are divided into four groups All Para set Error and Other including setting discriminators system self test and updating system software which are all listed on 8 22 Auto Hematology Analyzer Operation Manual V1 00 Maintenance the left of the screen All the recorded are listed on the right of the screen by default You can press F1 to select the interested group and the right of the screen will display the events of the selected group only Every screen displays 10 events You can press f or 1 to check the events one by one or press PgUp or PgDn to check the events on the previous or next screen If you want to print out the displayed events press PRINT If you want to acquire help information press HELP For every recorded event the NO column displays the sequences of the recorded events the Time column displays the time when this event occurred the Type column displays the event type the error events are
99. er for In Vitro Diagnostic Use in clinical laboratories Note The purpose of this analyzer is to identify the normal patient with all normal system generated parameters and to flag or identify patient results that require additional studies It determines the following 19 hematological parameters and presents 3 histograms of blood specimens Table 1 2 Parameters and histograms Group Parameter Abbreviation WBC Group White Blood Cell or leukocyte WBC Lymphocyte Lymph Mid sized cell Mid Granulocyte Gran Lymphocyte percentage Lymph Mid sized cell percentage Mid Granulocyte percentage Gran WBC histogram HGB Group Hemoglobin Concentration HGB RBC Group Hemoglobin Concentration RBC Hematocrit HCT Mean Corpuscular erythrocyte MCV Volume Mean Cell erythrocyte MCH Hemoglobin Mean Cell erythrocyte MCHC Hemoglobin Concentration Red Blood Cell erythrocyte RDW CV Distribution Width Coefficient of Variation Auto Hematology Analyzer Operation Manual V1 0 1 3 Introduction Red Blood Cell erythrocyte RDW SD Distribution Width Standard Deviation RBC histogram PLT Group Platele PLT Mean Platelet Volume MPV Platelet Distribution Width PDW Plateletcrit PCT PLT histogram 1 3 2 Classifications CE General In vitro diagnostic medical devices 1 3 3 Supplementary Data Mindray aperiodically
100. eration If there is any error present the system will not perform this procedure Follow the instructions displayed on the screen to remove the error before trying to perform the procedure again After draining the tubing follow the instructions displayed on the screen to put the rinse diluent and lyse tubes into distilled water and press ENTER to flush this analyzer with the distilled water When the washing is over follow the instructions displayed on the screen to remove the rinse diluent and lyse tubes from the distilled water and press ENTER to drain the tubing again Turn off the analyzer when the screen displays Turn off the analyzer Wipe this analyzer dry and wrap it up for storage Auto Hematology Analyzer Operation Manual V1 0 8 15 Maintenance 8 3 System Status The items displayed in the Status screen reflect how the system is functioning and contribute significantly to diagnosing system errors You may follow the instructions given below to check those items Press MENU to enter the system menu and press the appropriate arrow keys to move the cursor to Service gt Status as Figure 8 17 shows Whole Blood WBC HGB Current sample WBC 9 1 x 10 L Lymph 3 7 x 10 L i Mi ct o7x 1 We ID 118 Grand 4 7 x 10 L a Time 01 24 2004 13 55 Lymph H 41 0 Mid 8 0 Mode WB All 0 100 200 300fL s Gran 51 0 l HGB 124 g L Name RBC 4 08 x 10 2 L HCT L 36 0 y 88 3 fL Count
101. erformance of the analyzer by tracking the average red cell indices in the patient population analyzed on it The X B analysis demands random samples and therefore the samples categorized by diseases are not suitable for its use This X B analysis program analyzes the three above mentioned parameters on the patient samples run through this analyzer in batches of 20 4 2 1 Entering the X B Analysis Screen Press MENU to enter the system menu Press the appropriate arrow keys f lt to move the cursor to the Quality Control X B Analysis as Figure 4 14 shows WBC L 0 0 4 1 4 9 F Lymphit wee e 2 0 3 2 File No 1 Mick weet Lot No 7645 Gran eee F 4202220 Lymph ES i i Exp Date 07 27 2004 Mid we 8 Oo 100 200 300 fL Gran ed HGB L a 82 90 Total 4 RBC ee 2 60 3 00 Mode Whole Blood te Y 92152601 Coit V eee 78 0 90 0 l ttr t T T T T 27 9 31 9 0 50 150 250 fL Review 326 386 Quality control Setup 7 57 93 Service gt i Calibration 4 5 10 15 20 25 fL Shutdown Reagents for gt 99 Counts MENU T 1 lt 7 Select sub menu ENTER Enter sub menu Figure 4 14 Entering X B Table Screen 4 12 Auto Hematology Analyzer Operation Manual V1 0 Quality Control Press ENTER to enter the X B Table screen as Figure 4 15 shows X B analysis Ready 15 00 X B Table Reagents for gt 99 Counts MENU
102. es the QC program has been run The y coordinate represents the analysis results of the displayed parameters 2 For every parameter its L J graph presents maximum 31 points 3 For every parameter the upper dash line of its L J graph represents the upper limit of the expected range of the analysis result The corresponding value 10 5 in case of the WBC in Figure 4 9 equals Mean Range and is displayed on the left of the line Auto Hematology Analyzer Operation Manual V1 00 4 9 Quality Control 4 For every parameter the lower dash line of its L J graph represents the lower limit of the expected range of the analysis result The corresponding value 9 5 in case of the WBC in Figure 4 9 equals Mean Range is displayed on the left of the line 5 For every parameter its expected result 10 0 in case of the WBC in Figure 4 9 is displayed between the values of the upper dash line and of the lower dash line 6 For every parameter the three numbers on the right of its L J graph represents Mean the mean value of the saved results as the equation below defines i l n Mean where n represents how many times the QC program has been run and X is the result acquired from every QC analysis Diff standard deviation of the saved analysis results as the equation below defines where n represents how many times the QC program has been run and X is the result acquired from every QC analysis and Mean is the
103. eter in the upcoming X B analysis you must assign the mean and range to that parameter The mean is obtained by taking the average of the analysis results of a certain amount of patient samples best greater than 1 000 and vary depending on the regions The recommended range is between 5 and 10 of the mean Follow the steps given below to enter the mean and range to the Mean and Range columns respectively A Press ft or to move the cursor to Mean or Range of the parameter B Press lt or to move the cursor to the desired position within the field C Press PgUp or PgDn or the numeric keys on the external keyboard to enter a digit at the position where the cursor is located Note that this analyzer adopts an internal fixed decimal point Therefore you can just enter the digits without paying attention to the decimal point D To modify a digit at a certain position just move the cursor to that position and re enter the desired digit E When you are done entering the means or ranges press MENU to exit the X B Edit 4 14 Auto Hematology Analyzer Operation Manual V1 0 Quality Control screen Note that if the entered expected result is less than or equal to the corresponding range the system when exiting the X B Edit screen will warn the user that the entered values are invalid and automatically clears the Mean and Range columns 3 Exiting When you are done editing the X B settings follow the steps
104. evice Recorder Baud 9600 Gain Format Format 1 Parity None v Count Ref range Auto Handshake No x Other Off a Auto Off v Recorder Printer Reagents for gt 99 Counts MENU lt 7 Move cursor T Select setting item F1 Select setting group Figure 7 13 Auto printing Press T or J to move the cursor to On or Off and press ENTER to confirm the selection When you are done enabling or disabling the auto printing function press f or 4 to move the cursor to other printing settings you want to change 7 2 2 2 Changing Communication Settings You can change the communication settings to facilitate the data transmission from your analyzer to an external computer 1 Setting Baud Rate You may choose one of the four Baud rates for your analyzer 9600 4800 2400 or 1200 The 9600 is the most common setting Follow the steps given below to do so Atthe Setup screen press F1 to select the Print amp comm group Press T or J to move the cursor to the combo box right of Baud in the Transmit field Press ENTER to display the pull down menu as Figure 7 14shows 7 10 Auto Hematology Analyzer Operation Manual V1 00 Setup Setup Ready 21 20 Reagent Print Transmit Print amp comm Date amp time Device R
105. gents 3 Prime the diluent tubing with diluent as instructed by section 8 1 1 Diluent Prime Auto Hematology Analyzer Operation Manual V1 00 2 3 Installation 2 3 1 2 Connecting the rinse container Take out the rinse inlet tubing with a blue connector from the accessory kit Take out the rinse container in which there should be enough rinse Insert the tubing end that has no connector into the rinse container as Figure 2 2 shows and turn the container cover clockwise until secure Connect the tubing connector to the RINSE inlet blue on the back of BC 2800 and turn it clockwise until secure Blue tubing connector Figure 2 2 Rinse connector Do the following steps when the installation is done 1 Enter the rinse volume as instructed by section 7 2 1 1 Setting remaining volumes for reagents 2 Enter the expiration date as instructed by section 7 2 1 3 Setting expiration dates of reagents 3 Prime the rinse tubing with rinse as instructed by section 8 1 2 Rinse Prime 2 3 1 3 Connecting the lyse Take out the lyse inlet tubing with an orange connector from the accessory kit Take out the lyse container in which there should be enough lyse Insert the tubing end that has no connector into the lyse container as Figure 2 3 shows and turn the container cover clockwise until secure Connect the tubing connector to the LYSE inlet orange on the back of BC 2800 and turn it clockwise until secure 2 4 Auto Hemato
106. gure 8 2 Maintenance screen If you want to exit this screen press MENU to enter the system menu and access the desired screen from there Auto Hematology Analyzer Operation Manual V1 0 Maintenance 8 1 1 Diluent Prime Biohazard Wear standard laboratory attire glove and follow safe laboratory procedures when handling any material in the laboratory You should perform the Prime diluent procedure to prime the diluent tubing with diluent when there are bubbles in the tubing or the diluent in the tubing is contaminated or the diluent ran out and a new container of diluent is installed Follow the steps given below to do so At the Maintenance screen press the appropriate arrow keys T gt to move the cursor to Diluent Prime Press ENTER to prime the tubing with diluent and the priming progress will be displayed at the bottom of the screen as Figure 8 3 shows When the priming is done the screen will return to the initial state Service Ready 23 58 Diluent prime Lyse test Rinse prime Clean bath Lyse prime Drain bath Zap aperture Drain tubing Flush aperture Clean wipe block Probe cleanser cleaning Prepare to ship E Z cleanser cleaning Priming diluent Diluent prime Reagents for gt 99 Counts MENU T 1 lt 7 Select service item ENTER Execute Figure 8 3 Priming tubing with diluent 8 1 2 Rinse Prime Biohazard
107. h much water and go see a doctor for further treatment Auto Hematology Analyzer Operation Manual V1 0 8 1 Maintenance 8 1 2 Recommended Regular Maintenance Maintenance Period Content of Maintenance Everyday If you are to use this analyzer 24 hours a day be sure to perform the E Z cleanser cleaning procedure everyday Run the QC program everyday See Chapter 4 Quality Control for details Every three If you are to use this analyzer 24 hours a day be sure to perform the days Probe cleanser cleaning procedure every three days Every If you shut down your analyzer every day and follow the specified Week shutdown procedure to do that you need to perform the Probe cleanser cleaning procedure every week Every You should use the supplied sample probe localizer to calibrate the Month position of the sample probe to that of the wipe block The analysis result is sensitive to their alignment As needed When you think the bath might be dirty perform the Clean the bath procedure When the analyzed samples add up to 300 the system will remind the user to perform the Probe cleanser cleaning procedure When the analyzed samples add up to 4 000 the system will remind the user to perform the Clean wipe block procedure When this analyzer is not to be used for two weeks be sure to perform the Prepare to ship procedure to empty and wash the flow system To obtain reliable analysi
108. he analyzer and call Mindray Customer Service Department or the distributor 9 2 28 Rinse Out Possible causes insufficient rinse or wrong rinse volume setting Solution Check if there is sufficient rinse left If so access Setup gt Settings gt Reagents and adjust the remaining rinse volume as instructed by Chapter 7 2 1 1 if not change a new container of rinse as instructed by Chapter 2 3 9 2 29 Lyse Out Possible causes insufficient lyse or wrong lyse volume setting Solution Check if there is sufficient lyse left If so access Setup gt Settings gt Reagents and adjust the remaining lyse volume as instructed by Chapter 7 2 1 1 if not change a new container of lyse as instructed by Chapter 2 3 9 2 30 Diluent Out Possible causes insufficient diluent or wrong diluent volume setting Solution Check if there is sufficient diluent left If so access Setup gt Settings gt Reagents and adjust the remaining diluent volume as instructed by Chapter 7 2 1 1 if not change a new container of diluent as instructed by Chapter 2 3 9 2 31 Diluent Expired The diluent has expired Check whether the diluent has expired If so change a new container of diluent as instructed by Chapter 2 3 if not access Setup gt Settings gt Reagents and adjust the expiration date as instructed by Chapter 7 2 1 3 9 2 32 Pressure Error The vacuum chamber does not reach the expected pressure within the given time 9
109. he instructions given in the Chapter to calibrate this analyzer as needed Note The analyzer can provide valid data only after being calibrated 5 1 2 Quality of Calibration The calibration quality depends on the calibration materials and reagents You should use the calibrator and reagents recommended by Mindray You should properly store the calibrator and reagents Do not use expired calibrator or reagents Before calibration be sure to warm the calibrator to room temperature and well mix it Auto Hematology Analyzer Operation Manual V1 0 5 1 Calibration 5 2 Preparing for Calibration The BC 2800 can be calibrated by two calibrators the commercial calibrator and fresh blood When calibrated with the commercial calibrator or fresh blood the analyzer will automatically calculate the calibration factors and fill them to the Manual screen The BC 2800 provides two sets of calibration factors for the whole blood mode and prediluted mode respectively Do the following four checks before calibration If any of four checks fails do not proceed to the calibration 1 Check the analyzer and reagents Make sure the reagents are sufficient for the calibration and all the needed materials are prepared 2 Check the background as instructed by Section 3 7 Background check and make sure the result meets the requirements listed in table 3 4 3 At the Count screen analyze the control material for at least 10 times to ensure the
110. ial Port and the Data Transmission Format therefore providing detailed information for the software engineers to program Besides the user can conveniently perform transmission A 1 Connection The BC 2800 can be connected with an external computer through a DB9 connector The pins of the DB9 connector are shown in Figure A 1 Carrier Detect DCD Data Set Ready DSR Receive Data RXD Request to Send RTS Transmit Data TXD Clear to Send CTS Data Terminal Ready DTR Ring Indicator RI Signal Ground GND Figure A 1 DB9 connector BC 2800 can communicate with an external computer through its serial port 2 using the Pin 2 Pin 3 and Pin 5 of the DB9 connector A 2 Setting Transmission Parameters The data format is fixed for the transmission so that every byte to be transmitted has 7 data bits and 1 stop bit Enter Setup Settings and press F1 to select the Print amp comm group and edit the communication settings as instructed by Chapter 7 2 2 2 Auto Hematology Analyzer Operation Manual V1 00 A 1 Communication A 3 Transmission A 3 1 Transmission at Count Screen If the auto transmission function is on once the analysis is done the BC 2800 will automatically transmit the results to the external computer If the auto transmission function is off you can only transmit the results manually at the Review screen A 3 2 Transmission at Review Screen Select the results you want to transmit and tran
111. ill be transmitted to the external computer L1 Region L8 Region are LI L8 of eight histogram discriminators as shown in Figure A 2 Whole Blood All r uu L2 L3 L4 Current sample WBC 9 1 x 10 L TE i Lymph 3 7 x 10 L Mi ct 0 7 xao Wh ID 118 Gran 47x1 B Time 01 24 2004 13 55 Lymph H 41 0 i Di i Mid 8 0 Mode WB All 0 100 200 300 fL ee Gran 51 0 L5 L6 HGB 124 g L Name l RBC 4 08 x 1012 L i HCT L 36 0 i Gender MCV 88 3 fL i i Age MCH 30 3 pg 0 50 150 250 fL MCHC 344 g L L7 L8 Next sample RDW CV 13 6 i i RDVV SD 40 2 fL p i ID 119 PLT 222 x 10 L MPV 9 0 fL f M All i i ode WB A PDW 157 PCT 0 199 0 5 10 15 20 25 fL Reagents for gt 99 Counts MENU T 1 Histogram F1 F2 F3 Discriminator Shift ENTER Exit Figure A 2 L1 L8 demonstration A 4 3 Standard QC Data Format Standard QC Data Format as shown in Table A 3 Table A 3 Standard QC Data Format If handshake is selected ENQ If handshake is not selected STX Body of the text start Text Distinction Code B File No Lot No THAHHHT Month HH Day Year HHHH WBC 10 L HHHH RBC 10 L HHH HGB g L HHH Auto Hematology Analyzer Operation Manual V1 0 Communication PLT 10 L HHHH Lymph 10 L HHH AH Lymph HHH Gran 10 L HHH Gran HHH HCT HEH MCV fL HHEH MCH pg HHH MCHC g L HHHH WBC Limit 10 L HHHH RBC Limit 10 L H
112. ine V 88 3 fL i i H 30 3 pg 0 50 150 250 fL Review HC 344 g L W CV 13 6 i Quality control gt W SD 40 2 fL p i Setup gt L Ll Service gt DEEN TH Calibration a Auto 0 5 10 15 20 25 fL Shutdown Auto fresh blood Figure 5 3 Entering Manual from the system menu 2 Press ENTER to enter the Manual screen as Figure 5 4 shows Calibration Ready 15 48 Whole Blood Factor Time O Prediluted 02 0 01 27 2004 oo 01 27 2004 01 25 2004 01 25 20 01 27 20 Reagents for gt 99 Counts MENU F2 Exit Figure 5 4 Manual screen The left of the Manual screen displays the available calibration modes whole blood and prediluted The right of the Manual screen displays the calibration factors of WBC RBC 5 4 Auto Hematology Analyzer Operation Manual V1 0 Calibration HGB MCV and PLT and the time the factors are saved 5 3 3 Entering new calibration factors 1 Atthe Manual screen press F1 to select the calibration mode as you need 2 Calculate the new calibration factors per the following formula old factor x reference value new factor average of test value Example Assuming for a certain calibrator the expected WBC value namely the reference value mentioned in the formula above is 8 4 and the current whole blood calibration factor is 98 9 analyze this calibration material in the whole blood mode for five
113. ing the bed number press f or to move the cursor to the Auto Hematology Analyzer Operation Manual V1 0 3 11 Sample Analysis next field to be edited 7 To enter the name of the department from which the sample came you may A Press f or J to move the cursor to the edit box right of Dept B Use a keyboard to enter the name of the department similar to the way you enter patient names This edit box automatically saves the entered item to its pull down menu Figure3 7 which can be accessed by pressing ENTER Totally 30 items can be saved in the pull down menu and you may press f or to move the cursor to the interested item and press ENTER to select it C When you are done entering the department name press 1f or to move the cursor to the next filed to be edited Whole Blood All EE Enter sample information Cu ID 1 ID 00000119 Gender Male M Time 0 Name eet Mode Age 000 Years oo Months oo Days pO fL Chart No 90000000 Bed No 00000000 Name Dept Gender Sender Age Tester Oo fL Ne Checker ID 1 Mode r n PCT 0 199 0 5 10 15 20 25 fL Figure3 7 Select department name 8 To enter the name of the person who sent the sample for analysis you may A Press T or to move the cursor the edit box right of Sender
114. issues and delivers supplementary documents pertaining to operation or maintenance of this instrument Be sure to read and observe these documents carefully and precisely as well as insert them into this manual for convenient reference 1 3 4 Service Assistance If this analyzer does not operate properly follow the directions in Chapter 9 Troubleshooting If the error cannot be corrected by the recommended methods contact the Mindray Customer Service Department or the distributor 1 3 5 Reagents Calibrators and Controls The reagents controls calibrators and analyzer are components of a whole system and must be used as one Be sure that any products to be used have been tested and certified by the manufacturer Mindray recommends the reagents described in Chapter 1 5 10 Reagents Do not use the reagents or controls from different suppliers or else the analyzer may not perform as promised and even be damaged Each reagent should be examined prior to its application Product integrity may be compromised in damaged containers If a package appears damaged inspect the inside for signs of leakage or moisture and find them do not use the reagent Read the user s instructions of the reagents and operate as instructed when using the reagents Unless otherwise specified reagents must be stored and used at room temperature 1 4 Auto Hematology Analyzer Operation Manual V1 0 Introduction Note To properly and safely us
115. ists of two fields the left displaying the available functions the common users can only see Reprodu and the right displaying the 19 parameters and their reproducibility indices Mean Diff and CV If the selected samples are less than 3 the reproducibility indices are all 0 If the analysis result of certain parameter is invalid the corresponding index will also be invalid 6 16 Auto Hematology Analyzer Operation Manual V1 0 Sample Review 6 2 7 2 Trend After entering the administrator password see Chapter 7 1 Password you can enter the Special Functions screen open to administrators as Figure 6 22 shows Press F1 to access the Trend which displays the WBC RBC PLT HGB MCV and RDW CV trends of the select sample results The six trends are displayed in two screens three trends in one as Figure 6 23 and Figure 6 24 show You can press fT 1 to switch between screens The selected results are sequentially presented in the trend newest at the utmost left No 1 Review Ready 21 03 O Reprodu Trend ID 118 Time 01 24 2004 F2 Edit upper limit 1 Select parameter Reagents for gt 99 Counts MENU F1 Select the desired function Figure 6 23 Trend screen 1 Review Ready 21 04 Special Functions O Reprodu Trend
116. ive continuous times Take the average of the analysis results and compare it to the expected value and make sure the average is within the expected range specified by the data sheet of the calibrator or control If not contact Mindray customer service department Auto Hematology Analyzer Operation Manual V1 0 5 7 Calibration 5 4 Calibration Using Calibrators 5 4 1 Entering the Auto Calibration Screen Press MENU to enter the system menu Press the appropriate arrow keys to move the cursor to Calibration Auto as Figure 5 8 shows Calibration Nv al Whole Blood Para Factor Time Prediluted 7 WBC 00 0 01 27 2004 0 01 27 2004 0 01 25 2004 Count 0 01 25 2004 Review 01 27 2004 Quality control gt Setup Manual Auto fresh blood Service Calibration Shutdown Reagents for gt 99 Counts MENU T 1 lt 7 Select sub menu ENTER Enter sub menu Figure 5 8 Entering auto calibrator screen from system menu Press ENTER to enter the Auto calibration screen as Figure 5 9 shows Calibration Ready 15 52 Exp Date 01 27 2004 Whole Blood 1 2 3 4 5 Factor O Prediluted Reagents for gt 99 Counts MENU F2 Exit Figure 5 9 Auto calibrator screen 5 8 Auto Hematology Analyzer Operation Manual V
117. just move the cursor to that position and re enter the desired digit E When you are done entering the lot number press f or to move the cursor to the next item to be viewed or edited 3 Entering expiration date You should enter the expiration date of the control material to be used for the upcoming QC analysis in the edit box right of Exp Date Follow the steps given below to do so A Press ft or to move the cursor to the edit box B Press PgUp or PgDn to enter the digits for the expiration date in the MM DD YYYY format as Figure 4 6 shows WBC File No 1 ih Lot N Qc Edit File No 1 Lot No 7645 Exp Date W 27 i 2004 Exp F fL Para Mean Range Para Mean Range Total WBC Gran 0 Mode RBC Gran Time HGB HCT PLT MCV te Made Lyrmph MCH Lymph MCHC PDW 1 E PCT Oo 65 10 16 20 26 fL Reagents for gt 99 Counts pO EE Figure 4 6 Entering Exp Date C When you are done entering the expiration date press f or to move the cursor to the next item to be viewed or edited 4 Entering means and ranges To include a parameter in the upcoming QC analysis you should refer to the data sheet of the control material and find the listed mean and range of that parameter and then follow the steps given below to enter them to the Mean and Range respectively columns Follow the steps below to do so A Press f or to move the cu
118. keys on an external keyboard to enter a digit at the position where the cursor is located When you are done setting the ranges press F2 to save the changes as Figure 7 34 shows Press ENTER to confirm Setup Ready 23 50 Reagent Para Lower Upper Para Lower Upper Print amp comm ywec 004 0 10 0 MCV 82 95 0 ai amp time Lymph 08 4 0 MCH 31 0 Count Mid 0 1 0 9 MCHC 360 Ref range Gran 2 0 7 0 RDVV CV x 14 5 Other 56 0 300 11 0 17 0 282 oeo00000 Data saved successfully HCT 37 0 50 0 Group General v F2 Save F4 Default Reagents for gt 99 Counts MENU Figure 7 34 Changes saved f you want resume the default settings by the manufacturer press F4 7 2 6 3 Exiting editing reference ranges When you are done changing all the printing and transmission settings you want to change you may press F1 to select other setting group you want to change or Auto Hematology Analyzer Operation Manual V1 0 7 25 Setup press MENU and a dialog box will pop up to remind you to save the changes Press lt or gt to move the cursor to Yes and press ENTER to save the changes and exit to the system menu or move the cursor to No and press ENTER to exit to the system menu without saving the changes Setup Ready 23 51 Settings Reagent Para Lower Upper Para Lower Up
119. l be displayed at the bottom of the screen as Figure 8 6 shows When the zapping is done the screen will return to the initial state 8 6 Auto Hematology Analyzer Operation Manual V1 0 Maintenance Service Ready 00 00 Item Diluent prime Lyse test Rinse prime Clean bath Lyse prime Drain bath Drain tubing Flush aperture Clean wipe block Probe cleanser cleaning Prepare to ship E Z cleanser cleaning Zapping aperture Zap aperture Reagents for gt 99 Counts MENU T 1 lt 7 Select service item ENTER Execute Figure 8 6 Zapping aperture 8 1 5 Flushing Aperture You can perform the Flush aperture procedure to assist zapping the aperture Follow the steps given below to perform the procedure At the Maintenance screen press the appropriate arrow keys T L to move the cursor to Flush aperture Press ENTER to flush the aperture and the flushing progress will be displayed at the bottom of the screen as Figure 8 7shows When the flushing is done the screen will return to the initial state Service Ready 00 00 Item Diluent prime Lyse test Rinse prime Clean bath Lyse prime Drain bath Zap aperture Drain tubing Clean wipe block Probe cleanser cleaning Prepare to ship E Z cleanser cleaning Flushing aperture Flush aperture Reagents for gt 99 Counts MENU T 1 lt 7 Select servic
120. l the printing and transmission settings you want to change you may press F1 to select other setting group you want to change or press MENU and a dialog box will pop up to remind you to save the changes as Figure 7 18 shows Press or gt to move the cursor to Yes and press ENTER to save the changes and exit to the system menu or move the cursor to No and press ENTER to exit to the system menu without saving the changes Auto Hematology Analyzer Operation Manual V1 0 7 13 Setup Setup Settings Ready 23 42 Reagent Print amp comm Date amp time Gain Count Ref range Other CoO0000800 Transmit Off Cres Ea Recorder Format 2 Save changes Baud 9600 Parity None Handshake No Printer Reagents for gt 99 Counts MENU Figure 7 18 Saving changes 7 2 3 Date and Time You can set the system date and time as Figure 7 19 shows Follow the instructions given below to do so 7 2 3 1 Setup Ready 23 43 Coo00e00 Reagent Print amp comm Date amp time Gain Count Ref range Other Time 1 27 gt lt gt lt gt Hour Minute Second a gt lt gt lt gt
121. lid a dialog will pop up to warn you as Figure5 21 shows Press ENTER to exit the dialog box and clear the calibration result Calibration Ready 18 52 Auto fresh blood Invalid result Whole Blood Predi luted Factor 1S N Sample 1 Sample 2 Sample 3 Sample 4 Sample 5 Calculate oo0oo0oo0oo0oo0o ooe Reagents for gt 99 Counts MENU Figure5 21 Invalid result 3 After you have saved three calibration results this analyzer will automatically display the 5 18 Auto Hematology Analyzer Operation Manual V1 00 Calibration CV and calibration factors You can save maximum five calibration results as Figure5 22 shows Note that the CVs should be within the ranges specified in Table 5 1 Calibration Ready 18 58 Whole Blood Predi luted Para Mean 1 2 3 Factor Sample 1 Sample 2 Sample 3 Sample 4 Sample 5 Calculate ie 0 12 12 ie WBC RBC HGB MOCY PLT 45 43 45 43 3 29 104 7 Reagents for gt 99 Counts MENU F1 Select mode F2 Edit reference F3 Select sample Figure5 22 Calibration using fresh blood 4 Any factor that falls out of the calibration range will be flagged by a at the upper right corner When it happens you should try to find out the reason If the problem is beyond your ability contact Mindray customer service
122. ll so that no bubbles will be produced during the process Figure 3 10 Adding diluent 4 Remove the sample cup when the sample probe has risen up and press ENTER to exit the Add Diluent screen 5 Collect 20uL capillary blood specimen and immediately add it to the sample cup and shake to mix them 6 Pay particular attention to the following notes A When you are done mixing the prediluted sample capillary blood diluent mixture be sure to wait for 3 minutes before analyze it B When you are done mixing the prediluted sample be sure to analyze it within 40 minutes if you want to acquire reliable WBC differential results C If you are not interested in the differential result you can analyze the prediluted sample within two hours after it is ready D Be sure to prevent dusts from falling into the prepared diluent Otherwise you may acquire misleading result E The precision of the analysis results of the prediluted samples vary depending on the operators Mindray recommends every laboratory evaluate the stability of the results based on its sample quantity and collection method 3 16 Auto Hematology Analyzer Operation Manual V1 0 Sample Analysis 3 6 Sample Analysis To keep this analyzer precise be sure to run the QC program every day before beginning sample analysis See chapter 4 Quality Control for details 3 6 1 Analyzing Samples 3 6 1 1 Selecting Analysis Mode At the Count screen press MODE
123. logy Analyzer Operation Manual V1 0 1 1 Introduction 1 2 General See Table 1 1 for the organization of this manual and brief introductions of the chapters Table 1 1 Organization of the BC 2800 operation manual Chapters Contents Chapter 1 Introduction Introduces organization of this manual system introduction principles of operation specifications system operations and operation summary Chapter 2 Installation Introduces installation requirements and steps Chapter 3 Sample Analysis Introduces how to analyze samples on this analyzer Chapter 4 Quality Control Chapter 5 Calibration Introduces how to run the QC program on this analyzer Introduces how to calibrate this analyzer Chapter 6 Sample Review Chapter 7 Setup Introduces how to review search and print the saved sample analysis results Introduces how to set date time and other system settings Chapter 8 Service Introduces how to maintain this analyzer and replace the reagents Chapter 9 Troubleshooting Introduces how to deals with the reported errors Appendix A Communication Communication protocol Auto Hematology Analyzer Operation Manual V1 0 Introduction 1 3 System Introduction 1 3 1 Name and Intended Use The full name of this analyzer is BC 2800 Auto Hematology Analyzer It is a quantitative automated hematology analyzer and leukocyte differential count
124. logy Analyzer Operation Manual V1 0 Sample Review review the results saved in either of the databases you must first set that database as the Database by pressing T at the Review screen The sample results saved in either of the two databases are lined in the order of time the newest result always at Location 1 Figure 6 2 shows the first sample result saved in the Sample database which is housing 10 000 sample results Loc Total 1 9987 The ID of this sample is 118 and it was analyzed in the WB All mode and saved at 01 24 2004 13 56 If you want to print out the displayed result press PRINT If you want to acquire help information press HELP If you wan to switch to exit the Review screen press MENU and access the desired screen from there 6 1 1 Reviewing Result of a Specific Sample In the Histogram mode and at the Review screen if you want to review the result of a specific sample you may 1 Press lt or gt to move the cursor to the previous or next sample until the desired one is reached Or 2 Press PgUp or PgDn to jump backward or forward by 8 samples for example jump from location 1 to 9 or reversely until the desired one is reached Or 3 Follow the steps given below to go directly to the desired sample Press F1 to enter the Goto screen as Figure 6 3 shows Press lt or gt to move the cursor within the edit box right of Location and press PgUp or PgDn or the nume
125. logy Analyzer Operation Manual V1 00 Installation Figure 2 3 Lyse container Do the following steps when the installation is done Enter the lyse volume as instructed by section 7 2 1 1 Setting remaining volumes for reagents Enter the expiration date as instructed by section 7 2 1 3 Setting expiration dates of reagents Prime the lyse tubing with as instructed by section 8 1 3 Lyse Prime 2 3 1 4 Connecting the waste container Take out the waste inlet tubing with a red connector from the accessory kit Connect the tubing connector to the WASTE inlet red on the back of the analyzer and turn it clockwise until secure Place the waste container on a table as high as or lower than the analyzer Insert the other end of the tubing into the waste container Enter the volume of the waste container as instructed by section 7 2 1 2 Setting volume of dischargeable waste 2 3 2 Waste Disposal Biohazard Consider all materials specimens reagents controls calibrators or components that contain or have contacted human blood as being potentially infectious Wear standard laboratory attire glove and follow safe laboratory procedures when handling any material in the laboratory WARNING Handle and dispose of the waste according to acceptable laboratory local state and national standards Auto Hematology Analyzer Operation Manual V1 00 2 5 Installation 2 4 Loading Recording Paper Follow the steps given below to install
126. low the steps given below to exit the QC Edit screen A Press MENU and a dialog box will pop up to remind the user to save the changes as Figure 4 8 shows 4 6 Auto Hematology Analyzer Operation Manual V1 0 Quality Control Contrals Ready WBC File No 1 Pent Lot N QC Edit File No 1 Lot No 7645 Exp Date 07 27 2004 Exp NEL Para Mean Range Para Mean Range Note Total Mode RBC Save changes HGB Time ono Ka PET Made Lymph MCH Lymph MCHC PDW T T T T T PCT 0 6 10 15 20 25 fL Reagents for gt 99 Counts MENU Figure 4 8 Dialog Box to Confirm the Changes B To save the changes move the cursor to Yes and press ENTER otherwise move the cursor to No and press ENTER 6 Other operations A If you neither entered new settings nor changed the old settings you may press MENU to directly return to the Controls screen without seeing the dialog box shown in Figure 4 8 B If you want to print out the saved QC settings press PRINT Note newly entered or changed settings will not be printed out until they are saved C If you want to acquire help information press HELP 4 1 4 Running QC Before running the QC program be sure to read the instructions of the control material to be used and handle it as instructed Note that the new lot of controls should be analyzed in parallel with the current lot prior to their expiration dates for 4
127. lysis results to its internal memory When the Auto Hematology Analyzer Operation Manual V1 00 3 19 Sample Analysis memory is full the oldest result will be automatically covered by the newest one 3 20 Auto Hematology Analyzer Operation Manual V1 0 Sample Analysis 3 6 2 Operation Range of Sample Results Refer to Table 3 3 for the upper limit of the operation or printing range of the analysis Table 3 3 Upper limit of operation range or W range or ae ee range 499 9 x sonon OO oom O o fL Lymph 99 9 RDW SD 250 0fL 99 9 3000 x 10 09 9 RBC 9 99 x 10 L 30 0 fL HGB 300 g L 0 70 HCT 99 9 3 6 3 Parameter Flags If the analysis result of any parameter exceeds the reference range see section 7 2 6 you may see the following flags If the analysis result is followed by an H or L it means the analysis result has exceeded the upper or lower limit of the reference range If you see as opposed to the result it means the result either unreliable or out of the display range If WBC result is less than 0 5 x 10 L this analyzer will not perform the differential analysis and all the related parameter results will be marked 3 6 4 Histogram Flags The system will flag abnormal histograms 1 Abnormal WBC histograms will be flagged by one of the markings R19R2 R3 R4 and Rv A Ri Jindicates abnormality on the left side of the lymphocyte hump and possible
128. lyze the calibrator or control material for at least five continuous times Take the average of the analysis results and compare it to the expected value and make sure the average is within the expected range specified by the data sheet of the calibrator or control If not contact Mindray customer service department Auto Hematology Analyzer Operation Manual V1 0 5 15 Calibration 5 5 Calibration Using Fresh Blood You can prepare 3 5 fresh blood samples and test them on a reference analyzer for three times and take the averages as the reference values of the samples 5 5 1 Entering the Auto Fresh Blood Screen Press menu to enter the system menu Press the appropriate arrow keys 7 J gt to move the cursor to Calibration gt Auto fresh blood as Figure5 17 shows Service Result Count Review Quality control gt Setup gt Service Calibration Shutdown Self test Tubing Item x Daane Count Time O Circuit WEC s RBC s Aperture v Vacuum Normal Normal Auto fresh blood Reagents for gt 99 Counts MENU T 1 lt 7 Select sub menu ENTER Enter sub menu Figure5 17 Entering Auto fresh blood screen from system menu Press ENTER to enter the system menu as Figure5 18 Calibration Auto fresh blood Ready 18 45 Whole Blood Factor Predi luted Sa
129. mean value derived from the first equation CV Coefficient of Variation as the equation below defines CV Diff x 100 M ean where Mean is the mean value derived from the first equation and Diff is the standard deviation derived from the second equation 7 Every point in the graph is interpreted as follows The darkened square m that falls between the upper dash line and the lower dash line is within the control range Otherwise it is not The blank square o represents the QC analysis either ran into errors or is out of the display range 8 Other operations To print out the currently displayed L J graph press PRINT To acquire help information press HELP To return to the controls screen press MENU 4 1 5 2 QC Table At the Controls screen press F5 to enter the QC Table screen as Figure 4 12 shows where 4 10 Auto Hematology Analyzer Operation Manual V1 0 Quality Control every screen displays the results of 6 QC analysiss You can press PgUp or PgDn to switch to the previous or next screen to view the results of other saved QC analysis Contrals Ready 14 17 ile No al Lot No 7645 Exp Date 07 27 2004 Mea n Range 1 2 3 4 5 mir ime fec RBC HGB PLT g Lymph Lymph Gran Gran g HCT MCV MCH MCHC 14 47 4 5 2 87 86 90 56 6 1 5 01 27 04 14 49 4 5 2 81 85 92 55
130. meter the lower dash line of its X B graph represents the lower limit of the expected range of the analysis result The corresponding value 80 in case of the MCV in Figure 4 19 equals Mean Range is displayed on the left of the line 4 For every parameter its mean 90 in case of the MCV in Figure 4 19 is displayed between the values of the upper dash line and of the lower dash line 5 The time at which the sample was analyzed is displayed in the Time field 6 The current cursor position and the number of all the saved points are displayed in the Loc Totall field 7 To print out the displayed X B analysis results press PRINT To acquire help information press HELP To exit back to the X B table screen press MENU Auto Hematology Analyzer Operation Manual V1 0 4 17 Calibration Chapter 5 Calibration 5 1 Introduction The BC 2800 Auto Hematology Analyzer was calibrated before leaving the factory However errors may still be introduced to the system during transportation and applications Therefore you must calibrate this analyzer before using it for the first time and after every repair You may also calibrate this analyzer when you find system errors during the QC analysis You can calibrate this analyzer one of the three ways manually with calibrators and with fresh blood 5 1 1 Purpose of Calibration The purpose of calibrating the BC 2800 is to ensure the accuracy of the analysis results Be sure to follow t
131. mple 1 Sample 2 Sample 3 Sample 4 Sample 5 Calculate ie 0 12 0 ie Reagents for gt 99 Counts MENU F1 Select mode F2 Edit reference F3 Select sample Figure5 18 Auto fresh blood screen 5 16 Auto Hematology Analyzer Operation Manual V1 0 Calibration 5 5 2 Editing Reference Values 1 At the Auto fresh blood screen press F3 to select the whole blood or prediluted mode 2 When the mode is selected press F1 to choose the fresh blood sample whose reference values you want to set 3 Press F2 to enable the edit boxes in the Mean column as Figure5 19 shows Calibration Ready 18 46 Auto fresh blood Whole Blood Predi luted Factor Sample 1 Sample 2 Sample 3 Sample 4 Sample 6 Calculate ie ie e 0 0 O Reagents for gt 99 Counts MENU F2 Exit Figure5 19 Editing reference values 4 Press f or J to move the cursor to the edit box right of the parameter whose reference value you want to set press or gt to move the cursor within the box Press PgUp or PgDn or the numeric keys of a keyboard to enter digits to modify a digit move the cursor to the position of the digit and re enter the desired digit 5 Press F2 to quit editing 5 5 3 Calibration When you are done editing the calibration settings go to the Auto calibratio
132. n Date Format 0 0 ccc ee eeeeee ee eeee eee eeeaeeeeeeeeaeeeeeeeeeeteeeaeeeeeeaaees A 2 II Auto Hematology Analyzer Operation Manual V1 00 Introduction Chapter 1 Introduction 1 1 Foreword This manual covers all the instructions related to the operation and general maintenance of the BC 2800 Auto Hematology Analyzer To ensure this analyzer performs in the best way please carefully read and comprehend the contents of this manual and operate maintain this analyzer exactly as instructed This manual includes a detailed description of this analyzer the specifications given by the manufacturer methods to install calibrate and maintain this analyzer and measures to be taken when errors occur It also presents the working principle of this analyzer and the recommended reagents Operation notes limits and potential hazards are stressed where appropriate This chapter mainly deals with the following contents General Introduces how this manual is organized and presents a brief introduction of all the chapters System introduction Briefly introduces the main factors of this analyzer including functions accessories and optional parts Principles of operation Introduces how the analyzer functions Specifications Presents the specifications of this analyzer System operations Describes the key functions display areas and system menu and presents a brief introduction of how to operate this analyzer Auto Hemato
133. n Manual V1 00 9 3 Troubleshooting Possible causes clogged aperture inappropriate RBC count time settings or solenoid valve error Solution 1 Access Service gt Maintenance and do the Zap aperture and Flush aperture procedures as instructed by Chapters 8 2 4 and 8 2 5 2 After unclogging access Setup gt Settings gt Count to note down the preset RBC count time Access Service gt Self test gt Tubing and test the actual RBC count time as instructed by Chapter 8 4 1 If the tested time differs from the preset time by less than 2 seconds it means the unclogging is successful and you can return to the Count screen to continue the analysis Other wise Access Service gt Maintenance to soak the bath and tubing with probe cleanser as instruction by Chapter 8 2 6 When the soaking is done access Setup gt Settings gt Count to note down the preset RBC count time Access Service gt Self test gt Tubing and test the actual RBC count time as instructed by Chapter 8 4 1 If the tested time differs from the preset time by less than 2 seconds it means the unclogging is successful and you can return to the Count screen to continue the analysis If the difference is still greater than 2 seconds and stabilized around a certain value access Setup gt Settings gt Count to change the RBC count time accordingly After the adjustment test the actual count time again and make sure the difference is within 2 seconds 3 If the error
134. n Width RDW SD fL Standard Deviation Mean Platelet Volume MPV fL Platelet Distribution Width PDW Table 1 5 Calculated parameters Parameter Abbreviation Default Unit Lymphocyte Lymph 10 L Mid sized cell Mid 10 L Granulocyte Gran 10 L Hematocrit HCT Auto Hematology Analyzer Operation Manual V1 00 1 11 Introduction Mean Cell erythrocyte MCH pg Hemoglobin Mean Cell erythrocyte ae MCHC g L Hemoglobin Concentration Mean Platelet Volume PCT 1 5 2 Sampling Features 1 Sample volumes required for each analysis Whole Blood Mode vein blood Prediluted Mode capillary blood 2 Actually used sample volumes For a WBC analysis For a RBC PLT analysis 3 Lyse used for every analysis 4 Dilution rate Whole blood sample WBC HGB RBC PLT 5 Aperture size 6 Throughput 1 5 3 Performance 1 Display range Paranet er Wa 1071 Rad 10 L HA g L MV FI PEK 107 1 2 Linear range Paranet er Wq 1071 Rad 10 D HA g L PLI 107 1 3 Reproducibility 13uL 20p L 3001 L including diluent and lyse 3001 L including second dilution 0 5mL whole blood 0 36mL capillary blood Prediluted sample 1 417 1 45004 70um in length 1 308 1 44862 80u m in diameter More than 30 samples hour Qoerating range 0 0 499 9 0 9 99 300 0 0 250 0 2999 Linear range 0 0 99 9 0 0 9 99
135. n screen to analyzer the prepared fresh blood Biohazard Consider all materials specimens reagents controls calibrators or components that contain or have contacted human blood as being potentially infectious Wear standard laboratory attire glove and follow safe laboratory procedures when handling any material in the laboratory Warning The probe is sharp and may contain biohazard materials including controls and calibrators Avoid any unnecessary contact with the probe and probe area Auto Hematology Analyzer Operation Manual V1 0 5 17 Calibration 1 Press the START key to start the analysis and the progress bar is displayed 2 When the analysis ends ifthe obtained result is valid a dialog box will pop up to ask you confirm the calibration as Figure5 20 shows Calibration Ready 18 50 Auto fresh blood Is this calibration valid 1 Whole Blood Yes No Factor Predi luted 45 43 Sample 1 Sample 2 Sample 3 Sample 4 Sample 6 Calculate ie 0 12 12 ie Reagents for gt 99 Counts MENU Figure5 20 The dialog box to confirm the calibration is valid To save the result press gt to move the cursor to Yes and press ENTER and the result will be displayed on the screen otherwise move the cursor to No and press ENTER to discard the result If the obtained result is inva
136. nd this analyzer performs one of the power up procedures presented in Table 3 2 depending on the previous shutdown Table 3 2 Power up procedure Power up procedure Performing the startup procedure Resetting the motors checking the file system and performing the startup procedure 3 2 Power up 1 on 2 the screen displays Initialize 3 procedure Previous shutdown Normal shutdown Abnormal shutdown due to power failure or skipping the proper shutdown procedure Shutdown after the Prepare to ship procedure was done or after the tubing was drained 4 Priming the tubing cleaning the tubing repeatedly and performing the background check When the power up procedure is over the system automatically enters the Count screen If any error occurs during the power up procedure the corresponding error message will be displayed at the lower left corner of the screen If you want to access the Count screen from other screens follow the steps introduced in section 3 3 Entering Count Screen to do so Auto Hematology Analyzer Operation Manual V1 0 Sample Analysis 3 3 Entering Count Screen Press MENU to enter the system menu Press the appropriate arrow keys T lt to move the cursor to Count as Figure 3 1 shows Current sample WBC 0 0 x 10 L i Lymph see ex 10 L i Mi cit wees x 197 WI DH ee Grant aes xc toe BI Time 01
137. ns after you have tried the recommended method call Mindray Customer Service Department or the distributor This chapter consists of two parts the first part dealing with the errors and assigned error codes and the second possible causes and recommended solutions Auto Hematology Analyzer Operation Manual V1 0 9 1 Troubleshooting 9 1 Error Codes The errors recorded in the log are presented in the error codes See Table 9 1 for the correspondence between the errors and error codes Table 9 1 Errors and codes Envir Temp Background 0401 0402 0403 HGB error Abnormal abnormal 0404 HGB adjust 0405 WBC clog 0406 WBC bubbles 0407 RBC clog 0408 RBC bubbles Communication Scanner 0801 0802 Scanner error 0803 of be error communication error Printer out of Printer Recorder 1001 1002 i 1003 Rae paper connection error communication error Recorder out of 1004 1005 Recorder too hot 1006 Press bar up paper 2004 Lyse expired 2005 2006 Real time clock error Syringe motor Rotation motor 4004 Elevator motor error error error 400B Diluent out 400C 400D Waste full Dynamic 8001 File error 8002 memory error 9 2 Auto Hematology Analyzer Operation Manual V1 00 Troubleshooting 9 2 Solutions This chapter presents measures to be taken when the errors occur 9 2 1 A D Error Something is wrong with the A D converter on the CPU board Solution Access Service Self test Circuit Test the A D interru
138. nt Cannot edit RB HG MC Reagents for gt 99 Counts MENU Figure5 15 Reject edit dialog box If the valid results are less than three the CV and new factors are not available yet If you press F1 a dialog box will pop up to warn you about the data loss as Figure5 16 shows Calibration Ready 18 36 Lot No Exp Date 01 27 2 Whole Blood para Mean 3 4 Factor Prediluted WBC 4 5 RB Changing mode will lose data ves Ea HG MC Reagents for gt 99 Counts MENU Figure5 16 Warning dialog box To switch the modes press or gt to move the cursor to Yes and press ENTER and the saved data will be cleared otherwise move the cursor to No and press ENTER to return t the Auto Hematology Analyzer Operation Manualf V1 0 5 13 Calibration Auto calibration screen If you press MENU the system menu will pop up and the saved calibration results will be cleared At the Auto calibration screen when the CV and new factors are available you can press PRINT to print out the displayed information To acquire help information press HELP To return to the system menu press MENU 5 14 Auto Hematology Analyzer Operation Manual V1 0 Calibration 5 4 4 Testing new factors After the new factors are saved go to the Count screen to ana
139. nt Figure 4 1 Entering the Controls Screen from the system menu Press ENTER to enter the Controls screen as Figure 4 2 shows Controls WBC y Lymph File No 1 Midi Lot No Gran Lymph Exp Date 01 27 2004 Mid 0 160 200 300 fL Gran HGB RBC Mode HCT Time MCV T T T T T MCH 0 50 150 250 fL MCHC RDVW CV Mode VVhole Blood RDW 5D PET MPV PDW T T T T T PCT 0 6 10 15 20 25 fL Total 0 feagents for gt 99 Counts Figure 4 2 Controls Screen At the screen if you want to acquire help information press HELP If you want to delete the 4 2 Auto Hematology Analyzer Operation Manual V1 00 Quality Control displayed QC result follow the steps given below 1 Press DEL to enter the dialog box shown in Figure 4 3 WBC 9 5 8 8 10 0 Lyrnph 3 7 2 7 5 1 File No 1 Mick 07 Lot No 76450 Gran 5 1 4 1 6 1 Lymph 38 7 30 6 50 6 i i i Exp Date 07 10 2004 Mid TA D f Gran 54 1 46 1 60 1 1007 ee aoti Total 1 Mode Whole Blood Delete current QC results Time 01 25 2004 13 58 ves E D 150 250 FL RDW CY 14 2 Mode Whole Blood RDW 5D 40 9 PLT 242 220 280 MPY 9 1 PDW 15 7 PCT 0 220 0 65 10 15 20 25 fL Reagents for gt 99 Counts CEEE Pressure low Figure 4 3 Dialog Box to Confirm the Deletion 2 Press lt or to move the cursor to Yes and press ENTER if you want to confirm the deletion 3 Pre
140. nts MENU 7 Move cursor 7 Select setting item F1 Select setting group Figure 7 36 Selecting how to mute the beeper 7 2 7 2 Setting LCD contrast Follow the steps given below to adjust the LCD contrast Atthe Setup screen press F1 to select the Other group Press T or J to move the cursor to LCD contrast as Figure 7 37 shows Setup Ready 23 51 Settings Reagent Print amp comm Date amp time Gain Any key to mute Count Ref range Other Enabled LCD contrast 204 0000000 Alarm time s 10 PMB color Black X Note PMB means Prediluted mode background Reagents for gt 99 Counts MENU lt 7 Move cursor T Select setting item F1 Select setting group Figure 7 37 Setting LCD contrast Press PgUp or PgDn to adjust the contrast When you are done adjusting the contrast press T or 4 to move the cursor to other settings you want edit 7 2 7 3 Setting alarm time Follow the steps given below to set how long the error messages should be displayed on the screen 2s 120s Auto Hematology Analyzer Operation Manual V1 0 7 27 Setup At the Setup screen press F1 to select the Other group Press f or J to move the cursor to Alarm time as Figure 7 38 shows Setup Ready 23 56 Settings Reagent Print amp comm Date amp time
141. o move the cursor to the interested item and press ENTER to select it Press T or J to move the cursor to the next search condition to be specified To specify the ID of the sample you may Press T or J to move the cursor to the check box left of ID Press ENTER to tick the box Press 1 to move the cursor to the edit box right of ID Press or gt to move the cursor within the box and press PgUp or PgDn or the numeric keys of an external keyboard to enter the digit at the position where the cursor is located When you are done entering the sample ID press T or J to move the cursor to the next search condition to be specified To specify the bed number of the patient you may Press f or J to move the cursor to the check box of Bed No Press ENTER to tick the box Press J to move the cursor to the edit box right of Bed No Press or gt to move the cursor within the box and press PgUp or PgDn or the numeric keys of an external keyboard to enter the digit at the position where the cursor is located When you are done entering the bed number press f or 4 to move the cursor to the next search condition to be specified Auto Hematology Analyzer Operation Manual V1 0 6 13 Sample Review 6 e e e e N To specify the chart number of the patient you may Press T or J to move the cursor to the check box of Chart No Press ENTER to tick the box Pre
142. ock from the bottom as Figure 8 34 shows Auto Hematology Analyzer Operation Manual V1 0 8 29 Maintenance Figure 8 34 Using localizer 8 Insert the probe into the wipe block until it reaches the localizer as Figure 8 35 shows Figure 8 35 Inserting sample probe into wipe block 9 Retighten the screw to fix the probe and remove the localizer to wrap up the adjustment 8 8 2 Replacing Probe Wipe Block Biohazard Wear standard laboratory attire glove and follow safe laboratory procedures when handling any material in the laboratory Warning The probe is sharp and may contain biohazard materials including controls and calibrators Avoid any unnecessary contact with the probe and probe area Follow he steps given below to replace the probe wipe block 1 Refer to Chapter 8 7 1 and do the steps 1 6 8 30 Auto Hematology Analyzer Operation Manual V1 00 Maintenance 2 Pull the loosen sample probe upward to remove the wipe block and disconnect the tubes from the wipe block pay attention to the correspondence between the tubes and the connectors as Figure 8 36 shows 3 Refer to Chapter 8 7 1 and do the steps 7 9 of fix the sample probe Figure 8 36 Installing wipe block Auto Hematology Analyzer Operation Manual V1 0 8 31 Troubleshooting Chapter 9 Troubleshooting The chapter deals with the codes possible causes and solutions of the errors If the error remai
143. or Lower sensor 3 Counting start when the 4 Counting end when the liquid liquid surface pass the upper surface pass the lower sensor sensor Figure 1 3 How sample volume is controlled 1 4 4 Derivation of Parameters 1 4 4 1 WBC WBC 10 L is the number of leukocytes measured directly by counting the white blood cells passing through the aperture Note that when you observe in the microscope NRBCs nucleated red blood cells which do not react with the lyse and can be mistaken by the analyzer for white cells be sure to correct the system generated result by the following formula 100 WBC WBC x ____ 100 NRBC where WBC represents the system generated white cell number NRBC the number of NRBCs counted in 100 white cells and WBC the corrected white cell number 1 4 4 2 WBC differential With the help of the diluent and lyse this analyze can size the white cells into three sub populations lymphocytes mid sized cells and granulocytes Lymphocytes are the largest white cells whose sizes are 30fL 85fL Granulocytes are the smallest white cells whose sizes are above 125fL Between them are the mid sized cells whose sizes are 85fL 125fL 1 8 Auto Hematology Analyzer Operation Manual V1 0 Introduction Based on the WBC histogram this analyzer calculates Lymph Mid and Gran as follows PL Lymph x 100 pias oX
144. or changeable setting groups Press F1 to select the desired group The selected group is preceded bya 10 Setting area You can change the settings of the items of the selected group in this area 11 Help area This area displays useful information to help the user to move to the next step At this screen if you want to acquire help information press HELP If you want to return to the system menu press MENU 7 2 1 Reagents You can change the settings regarding the reagents and the waste at the Setup screen as Figure 7 7 shows Setup Waiting 21 10 Settings Reagent Remains Remains Print amp comm Date amp time Diluent Gain Rinse Waste Count Container 00 00 Ref range Lyse Other Exp Dat Diluent Rinse Lyse Rinse out Reagents for 0 Counts MENU lt 7 Move cursor T Select setting item F1 Select setting group Figure 7 7 Reagent settings 7 2 1 1 Setting remaining volumes for reagents You may set the remaining volumes for the diluent rinse and lyse When any of the entered volumes is counted down to zero the system will remind the user to replace the corresponding reagent 1 Atthe Setup screen press F1 to select Reagent group 2 Press f or J to move the cursor to the edit boxes right of Diluent Rinse or Lyse in the Remian
145. or to General Male adults Female adults Pediatric patients or Neonates and press ENTER to confirm the selection When you are done selecting the patient group press T or J to move the cursor to the parameter whose reference range you want to view or change 7 2 6 2 Setting reference ranges administrators only For the General Male adults Female adults and Pediatric patients groups the manufacturer recommended references are available for all the 19 parameters As for the Neonates the manufacturer recommended references are only available for the following 5 parameters WBC Lymph RBC HGB and PLT You may enter or change the references to your own need Follow the steps given below to do so Obtain the administrator authority as instructed in Chapter7 1 1 Atthe Setup screen press F1 to select the Ref range group Press ft or J to move the cursor to General Male adults Female adults Pediatric patients or Neonates and press ENTER to confirm the selection Press ft or to move the cursor to the upper or lower limit of the parameter for instancet WBC whose reference range you want to change See Figure 7 32 Setup Ready 23 50 Settings Reagent Para Lower Upper Para Lower Upper Print amp comm WBC j 0 ian aor 010 0 MCV 82 i 95 0 Sain Lymphit 4 0 MCH 7 0 31 0 Count Mid 0 9 MCHC 360 Ref range Gran s 7 0 RDW CY 14
146. or to No and press ENTER to ignore the message Auto Hematology Analyzer Operation Manual V1 0 8 13 Maintenance Count Whole Blood All Ready 00 18 Current sample 121 01 100 200 Reagents for gt 99 Counts MENU Figure 8 15 Recommending probe cleanser cleaning 8 1 13 Preparing to Ship If this analyzer is not to be used for over two weeks or is to be shipped perform the Prepare to ship procedure to flush and drain it Follow the steps given below to do so At the Maintenance screen press the appropriate arrow keys T gt to move the cursor to Prepare to ship Remove the diluent rinse and lyse tubes from their containers Press ENTER and a dialog box will pop up to ask you to confirm this operation as Figure 8 16 shows Service Ready Diluent prime Rinse prime Lyse prime Flush aperture Probe cleanser c E Z cleanser cle Remove the tubin Prepare to ship Lyse test Clean bath Drain bath Z aein i E block Yes No e and lyse containers 00 13 Reagents for gt 99 Counts MENU Figure 8 16 A dialog box of confirmation Move the cursor to Yes and press ENTER if you want to proceed with this procedure or 8 14 Auto Hematology Analyzer Operation Manual V1 0 Maintenance to No and press ENTER if you wan to abort this op
147. pected value to it similar to enter the lot number Press F2 to quit editing 5 4 3 Calibration 1 When you are done editing the calibration settings go to the Auto calibration screen to analyzer the prepared calibrator Auto Hematology Analyzer Operation Manual V1 0 5 9 Calibration Biohazard Consider all materials specimens reagents controls calibrators or components that contain or have contacted human blood as being potentially infectious Wear standard laboratory attire glove and follow safe laboratory procedures when handling any material in the laboratory Warning The probe is sharp and may contain biohazard materials including controls and calibrators Avoid any unnecessary contact with the probe and probe area 2 Press the START key to start the analysis and the progress bar is displayed 3 When the analysis ends lf the obtained result is valid a dialog box will pop up to ask you confirm the calibration as Figure 5 11 shows Calibration Ready 15 58 Is this calibration valid 1 Whole Blood O Prediluted Factor Reagents for gt 99 Counts MENU Figure 5 11 The dialog box to confirm the calibration is valid To save the result press gt to move the cursor to Yes and press ENTER and the result will be displayed on the screen otherwise move the cursor to No and press ENTER to disc
148. per Print amp comm lwec 003 0 10 0 MCV 82 0 95 0 ee time Lympht 08 40 MCH 7 0 31 0 Beas Mid 0 1 0 9 MCHC 320 360 Ref range Grant 2 0 7 0 _RDW CV 14 5 Other D 35 0 56 300 7 0 11 Yes I 15 0 17 HCT 37 0 50 0 Group General j Save changes F2 Save F4 Default Reagents for gt 99 Counts MENU Figure 7 35 Saving changes 7 2 7 Other Settings There are also other settings you can view or change Follow the instructions given below to do so 7 2 7 4 Muting beeper This analyzer beeps when errors occur You can mute the beeper by pressing any key or leave the beeper beeping unit the errors are removed Follow the steps given below to do so Atthe Setup screen press F1 to select the Other group Press fT or to move the cursor to the edit box right of Pressing any key to mute the beeper Press ENTER to display the pull down menu as Figure 7 36 shows Press T or J to move the cursor to other settings you want to edit 7 26 Auto Hematology Analyzer Operation Manual V1 0 Setup Setup Settings Ready 23 51 Reagent Print amp comm Date amp time Gain Count Ref range Other Any key to mute LCD contrast Alarm time s Disabled Enabled PMB color Note PMB means Prediluted mode background Reagents for gt 99 Cou
149. play Auto Hematology Analyzer Operation Manual V1 00 1 6 5 System Menu See Figure 1 10 for the structure of the system menu Count Revi ew Quality control Control s X B analysis Setup Password Settings Servi ce Mi nt enance Stat us Sel f test Log Oonfi g Pri nt Gali bration Mnual Auto Auto fresh bl ood Shut down Figure 1 10 System menu Introduction Auto Hematology Analyzer Operation Manual V1 0 Introduction 1 7 Operation Summary The BC 2800 can analyze two types of blood specimens whole blood samples and prediluted blood samples See Table 1 7 Table 1 7 Operation summary Do preliminary checks reagent and waste containers tubing connections power connections and the like Turn on the analyzer power The system begins self test and does the startup procedure and then enters the Count screen if every thing is normal Select appropriate count mode and prepare the samples to be analyzed Present the sample to the sample probe and press the START key for aspiration The system will automatically analyzes the sample and displays the results Edit patient information optional Print out analysis result Do the shutdown procedure Turn off the power 1 22 Auto Hematology Analyzer Operation Manual
150. power when the message Turn off the analyzer appears Reagents for gt 99 Counts Figure 3 18 Shutdown screen Biohazard Consider all materials specimens reagents controls calibrators or components that contain or have contacted human blood as being potentially infectious Wear standard laboratory attire glove and follow safe laboratory procedures when handling any material in the laboratory Warning The probe is sharp and may contain biohazard materials including controls and calibrators Avoid any unnecessary contact with the probe and probe area Auto Hematology Analyzer Operation Manual V1 0 3 27 Sample Analysis 4 Present the E Z cleanser to the sample probe and press the START key to aspirate 1 6mL cleanser The screen then displays the count down to turn off the analyzer as Figure 3 19 shows Shutdown Shutdown EL 26 Reagents for gt 99 Counts fee O RN Figure 3 19 Count down to turn off the analyzer 5 When the Shutdown procedure is done the screen displays Turn off the analyzer Turn off the analyzer as instructed 6 Dispose of the waste Biohazard Consider all materials specimens controls calibrators or components that contain or have contacted human blood as being potentially infectious Wear standard laboratory attire glove and follow safe laboratory procedures when handling any material in the laborator
151. presence of platelets coagulate large platelet nucleated red cell insolvable red cell protein lipoid debris in sample or electrical noise B R2 indicates abnormality between the lymphocyte hump and the mononuclear area and Auto Hematology Analyzer Operation Manual V1 0 3 21 Sample Analysis possible presence of atypical lymphocyte original cell in the sample and increased eosinophil or increased basophil C R23 indicates abnormality between the mononuclear leukocyte and the neutrophilic granulocytes and possible presence of immature granulocytes abnormal sub population in the sample or increased eosinophil D R4 indicates abnormality on the right side of the neutrophilic granulocytes hump and increased absolute number of neutrophilic granulocyte E RaO indicates at least two R flags 2 Abnormal PLT histograms will be flagged by one of the markings PaO Ps and P A Pm indicates blur demarcation between the platelet and red blood cell area and possible presence of large platelet platelet coagulation small red blood cell cell debris or fibrin B Ps indicates excessive small PLTs C P O indicates excessive large PLTs rc 3 6 5 Adjusting Histograms Manually You can adjust the histogram discriminators if you are unhappy with the current WBC differential or RBC PLT results Note that you cannot adjust the dis
152. pt as instructed by Chapter 8 4 4 The error will be cleared if the test result is normal Otherwise call Mindray Customer Service Department or the distributor 9 2 2 Dynamic Memory Error Something is wrong with the system memory Turn off the analyzer and call Mindray Customer Service Department or the distributor 9 2 3 HGB Error HGB gain is not correct and HGB blank voltage is within OV 3 2V or 4 9V 5V Solution Access Setup gt Password to gain the administrator authority as instructed by Chapter 7 1 1 Access Setup gt Settings gt Gain to adjust the HGB blank voltage to 3 4 4 8V 4 5V recommended as instructed by Chapter 7 2 4 3 If the error still remains turn off this analyzer and call Mindray Customer Service Department or the distributor 9 2 4 HGB Adjustment HGB gain is not correct and HGB blank voltage is within 3 2 3 4V or 4 8 4 9V Solution Access Setup gt Password to gain the administrator authority as instructed by Chapter 7 1 1 Access Setup gt Settings gt Gain to adjust the HGB blank voltage to 3 40 4 8V 4 5V recommended as instructed by Chapter 7 2 4 3 If the error still remains turn off this analyzer and call Mindray Customer Service Department or the distributor 9 2 5 RBC Clog This error message occurs when the actual RBC count time is greater than the preset RBC count time by 2 seconds Auto Hematology Analyzer Operatio
153. r PgDn or the numeric keys on a keyboard to enter a digit at the position where the cursor is located Note only the digit at which the cursor is located displays the true value and others are covered by 4 When you are done entering the password press ENTER to save the password to the system as Figure 7 3 shows Setup Ready 21 06 Password geet PW level Administrator Reagents for gt 99 Counts MENU Figure 7 3 Obtain administrator authority 5 Press MENU to return to the system menu as the administrator 7 2 Auto Hematology Analyzer Operation Manual V1 0 Setup 7 1 2 Giving Up Administrator Authority 6 Press MENU to enter the system menu Press the appropriate arrow keys T JL gt to move the cursor to Setup Password 7 Press ENTER to enter the Password screen in which the default common user password is displayed 8 Press ENTER to save this password to the system as Figure 7 4 shows Setup Ready 21 07 Password ott PW level User Reagents for gt 99 Counts MENU Figure 7 4 Obtaining common user authority 9 Press MENU to return to the system menu as the common user Auto Hematology Analyzer Operation Manual V1 0 7 3 Setup 7 2 Editing Settings You nany change the systemsettings to your ow need as introduced in the followng Chapters Press MENU
154. r Message Area The Error Message area displays error messages one by one switching every two seconds Auto Hematology Analyzer Operation Manual V1 0 3 5 Sample Analysis 3 3 8 Reagents Status Area The Reagents Status area displays how many counts the remaining reagents are enough for Note that when it displays 99 counts it indicates both the reagents are enough for over 99 counts and there is also enough space left in the waste container for the counts when it displays 0 counts it indicates either at least one of the reagents is insufficient or the waste container is full 3 3 9 Menu Area When you press MENU this area displays the system menu 3 3 10 Help Area The Help area reminds you how to proceed to the next step 3 6 Auto Hematology Analyzer Operation Manual V1 0 Sample Analysis 3 4 Entering Sample Information You may enter sample information in any of the three modes ID only All info and Batch edit depending on the configuration of your analyzer Note that the All info mode cannot co exist with the other two modes 3 4 1 ID Only Mode If your analyzer is configured as ID only it means you can only enter the sample IDs into this analyzer Follow either of the ways given below to do so 1 If you have a barcode scanner connected to your analyzer you may enter the bar coded sample ID at the Count screen or the Next sample screen simply by presenting the bar code to the scanner see the user
155. ray Customer Services Department or the distributor Otherwise the service life of this analyzer may be shortened 8 1 Regular Maintenance To keep this analyzer in a good shape regular maintenance is demanded 8 1 1 Special Notes The parts in contact with blood are potentially infectious Be sure to wear standard laboratory attire including rubber gloves when maintaining or operating this analyzer and wash your hands with detergent when you are done Be sure to keep you hair clothes cuff or hands away from the moving parts of this analyzer Be sure to use specified tools or parts to maintain this analyzer and be sure to clean the used tools as instructed by their instruction manual when you are done Be sure to use soft and clean cloth or neutral detergent soaked cloth twisted dry or soft cloth washed by ethanol to clean the surface of this analyzer Be sure to pay attention to the marks or symbols on this analyzer Be sure not to touch the power plug at the back of this analyzer with wet hands or wet rags Be sure not use organic solvent or acid alkaline detergent to wash the surface of this analyzer Otherwise the surface may fade or become corrupted Be sure to avoid direct contact with the reagents that will hurt your eyes skin and diaphragm In case you spill the reagents on you skin be sure to wash them off with much water In case you spill the reagents into your eyes be sure to immediately wash your eyes wit
156. remove the right plate of this analyzer 2 Access the Setup Password screen and enter the administrator password to obtain the administrator authority see Chapter 7 1 1 Access the Service Self test screen 3 Press F1 to select the Machine group and press f or J to move the cursor to Elevator motor as Figure 8 30 shows Auto Hematology Analyzer Operation Manual V1 0 8 27 Maintenance Service Ready 00 37 Machine O Valve O Circuit Item Syringe motor Rotation motor Elevator motor Reagents for gt 99 Counts MENU F1 Select test group f Select test ENTER Execute Figure 8 30Testing elevator motor 4 Press ENTER and an elevator motor screen will pop up as Figure 8 31 shows Service Ready 00 37 O Tubing Item Result Machine O Valve O Circuit Syringe motor Rotation motor Reagents for gt 99 Counts MENU Figure 8 31 Elevator motor screen 5 Press T to move the sample probe upward and press gt to move the probe to above the bath as Figure 8 32 shows 8 28 Auto Hematology Analyzer Operation Manual V1 0 Maintenance Figure 8 32 Sample probe above the bath 6 Loose the retaining screw by a screwdriver as Figure 8 33 shows Figure 8 33 Removing screws 7 Remove the probe from the wipe block and insert the localizer into the wipe bl
157. ric keys on a keyboard to enter a digit at the position where the cursor is located When you are done entering the target location press ENTER to return to the Review screen to see the selected result Review Ready 20 38 200 300 fL B Location Reagents for gt 99 Counts MENU Figure 6 3 Goto screen Auto Hematology Analyzer Operation Manual V1 0 6 3 Sample Review 6 1 2 Editing Sample Information if configured In the Histogram mode and at the Review screen you may edit the sample information of the currently displayed sample Press F2 to enter the Edit sample info screen as Figure 6 4 shows Follow the steps introduced in Chapter 2 5 1 to edit and save the sample information except for the ID which is for reviewing only and should not be changed Review Ready 20 38 Edit sample information Gender 000 Years Chart No 00000000 Dept Sender Tester Checker 0 199 Reagents for gt 99 Counts MENU Figure 6 4 Sample edit screen 6 1 3 Adjusting Histograms In the Histogram mode and at the Review screen you can also follow the steps introduced in Chapter 2 7 5 to adjust the displayed histograms manually 6 4 Auto Hematology An
158. rmally 3 Elevator motor The elevator motor controls elevation of the sample probe This test checks whether the motor functions normally 4 Print This test checks whether the recorder or printer functions normally If normal when you can press ENTER the recorder or printer will print out Printer recorder test if abnormal the screen will display the corresponding error message and you can see Chapter 9 Troubleshooting for solutions 8 3 3 Testing Valves Malfunctioning valves will lead to malfunctions of the tubing Therefore testing the valves is a major way to remove fluidics errors At the Self test screen press F1 to select the Valve group as Figure 8 22 shows The following test items will be displayed on the right of the screen Press the appropriate arrow keys T to select the valve you want to check and press ENTER to test it If the valve goes through the Off On Off sequence without making abnormal sound it passes the 8 20 Auto Hematology Analyzer Operation Manual V1 0 test Otherwise something may be wrong with the valve Maintenance Service Ready 00 33 O Tubing O Machine Valve O Circuit Item Result Item Result Off Off Off Off Off Off Vi Off Va Off vg V10 Off Off Reagents for gt 99 Counts MENU F1 5elect test group T Select test ENTER Execute 8 3 4 Testing Circuits Figure 8 22Testing valves
159. rsor to Mean or Range of the parameter to be included in the QC analysis Auto Hematology Analyzer Operation Manual V1 0 4 5 Quality Control Press or gt to move the cursor to the desired position within the column Press PgUp or PgDn to enter a digit at the position where the cursor is located as Figure 4 7 shows Note that this analyzer adopts a fixed decimal point Therefore you can just enter the digits without paying attention to the decimal point WBC File No 1 Lymph Lot N File No 1 Lot No 7645 Exp Date 07 27 2004 Exp fL Para Mean Range Para Mean Range Total WBC 9 4 0 6 Gran 0 Mode RBC 0 00 Gran HGB HCT Time E PLT MCV fL Made Lymph MCH Lymph MCHC PDW l T T T T T PCT 0 6 10 15 20 25 fL Reagents for gt 99 Counts Eere N EN Figure 4 7 Entering Mean and Range D To modify a digit at a certain position just move the cursor to that position and re enter the desired digit E When you are done entering the means or ranges press f or to move the cursor to the next item to be viewed or edited Note that if the entered mean is less than or equal to the entered range the system when exiting the QC Edit screen will warn the user that the entered values are invalid and automatically clear the corresponding Mean and Range columms 5 Exiting the QC Edit screen When you are done editing the QC settings fol
160. rsor to other settings you want to change 7 2 7 5 Exiting editing other settings When you are done changing all the printing and transmission settings you want to change you may press F1 to select other setting group you want to change or press MENU and a dialog box will pop up to remind you to save the changes as Figure 40 shows Press lt or gt to move the cursor to Yes and press ENTER to save the changes and exit to the system menu or move the cursor to No and press ENTER to exit to the system menu without saving the changes Setup Ready 23 56 Settings Reagent Print amp comm Date amp time Gain Any key to mute Enabled Count Ref range Other LCD contrast 204 Save changes ves EM mode background Reagents for gt 99 Counts MENU Figure 7 40 Saving changes Auto Hematology Analyzer Operation Manual V1 00 7 29 Maintenance Chapter 8 Maintenance Regular cleaning and maintenance are demanded to guarantee this analyzer operating properly This chapter introduces how to take care of this analyzer and check the system status Liquid overflow or leak during the operation of this analyzer will degrade the accuracy of the analysis results Once it occurs immediately wipe off the spills If it occurs inside this analyzer be sure to shut down the power immediately and call Mind
161. s MENU Figure 8 28 Print screen Auto Hematology Analyzer Operation Manual V1 00 8 25 Maintenance You can perform the following operations at the Print screen Press ft or to select the desired task Press DEL to delete the selected task Press HELP to display the help information Press MENU to return to the system menu 8 26 Auto Hematology Analyzer Operation Manual V1 0 Maintenance 8 8 Adjusting Sample Probe and Probe Wipe Block The relative position between the sample probe and probe wipe block has influence on the analysis results In the accessory box there is a sample probe localizer as Figure 8 30 shows You need to use the localizer to adjust the position of the sample probe if you have replaced wipe block or observed motor error or wrong analysis result Also as required by regular maintenance you should use the localizer to adjust the position of the sample probe monthly Figure 8 29 Probe localizer 8 8 1 Adjusting Sample Probe Position Biohazard Wear standard laboratory attire glove and follow safe laboratory procedures when handling any material in the laboratory Warning The probe is sharp and may contain biohazard materials including controls and calibrators Avoid any unnecessary contact with the probe and probe area Follow the steps given below to adjust the sample probe position 1 Refer to Chapter 8 1 8 and follow the steps 1 and 2 to
162. s a eR Rede OR RR oa ww aah 7 1 TA PassWord oera pret cubated breed teh oteee tug AEE OAE EE abd E ERE oteck canes 7 1 Ta Editing Settings 2 2 40 nee ia Ata ata ha eat ad aya teete a a 7 4 Chapter 8 Maintenances 2ciiy So i belo ie ttida te aee telex sausas 8 1 821 Regular Maintemance aeniea aeieea elite ce RSKR ENa AAEN elite Hib eek ieee ety 8 1 8 2 System Maintenance 00 ccccccccccececeeeeeceeeee cence eeseaeeeeeeeseeeeeseaeeseaeeseeeeessaeeesaeeneaes 8 3 8 3 System Status n r atti e e tera tea ati ene eee 8 16 8 4 System Self Test cccccccc cece eeeneeceeeeeceeeeeeeaeseeeee scenes caeeeeeaesseaeeeseeeeeaeseeaeeseeeees 8 18 tSo LOG AEEA EE A E EE E 8 22 8 6 System Configuration eeir ee eceeeeeeeeseeeeeeaeeseeeeseeeeeseaeessaeeseeeseeeees 8 24 8 7 Printing Management cece eeeeee ee eeeeeeeeeeeeeaeeeseaaeeeseeaaeeeeeeaaeeeeeeaaeeeenenaees 8 25 8 8 Adjusting Sample Probe and Probe Wipe Block c cccccesseeessesteeeseeneeees 8 27 Chapter 9 Troubleshooting s crs mareen ran a ee ee eee eee e eens 91 SAIM EFRON COGCS EA EA EA A E TETA 9 2 oT o a EE EE eee E E ee tee ee A a A A 9 3 Appendix A Communication 0 0 0 cece cece eee eee eens A Aad GONNE HOM ales aaar aara aaa aa tbh pst T AA Ea a EAE aKT TASER A A 1 A 2 Setting Transmission Parameters cccccccseseeceeeeeeeeeeeeaeeseeeeesaeeeeaeeeeneeeeas A 1 Ao IFANSMISSION enea a a e ate ae a a anid EE A N A 2 A 4 Transmissio
163. s field 3 Press lt or gt to move the cursor within the edit box right of the desired reagent Press PgUp or PgDn or the numeric keys on a keyboard to enter a digit at the position where the Auto Hematology Analyzer Operation Manual V1 0 7 5 Setup cursor is located Note that this analyzer has an internal fixed decimal point and you can just enter the digits ignoring the decimal point See Table 7 1 for the valid reagent volumes Table 7 1 Valid reagents volumes Diluent Rinse Lyse Allowed range 0 30 00 L 0 30 00 L 0 999 mL 4 When you are done entering the remaining volume press T or 4 to move the cursor to other settings to be changed 7 2 1 2 Entering usable volume of the waste container You may enter the usable volume of the waste container When the system counts down the entered volume to 0 it will alert the user to empty the waste container Follow the steps given below to set the volume 1 Atthe Setup screen press F1 to select the Reagent group 2 Press f or J to move the cursor to the edit box right of Container 3 Press or gt to move the cursor within the edit box Press PgUp or PgDn or the numeric keys on a keyboard to enter a digit at the position where the cursor is located Note that this analyzer has an internal fixed decimal point and you can just enter the digits ignoring
164. s for the aspirated fluid flows from the first sensor to the second 2 Count Time RBC S It measures the duration of a RBC count namely how many seconds it takes for the aspirated fluid flows from the first sensor to the second 3 Aperture v It measures the voltage v over the aperture 4 Vacuum It checks whether the vacuum system functions normally 5 Pressure It checks whether the system flushes the aperture at a normal pressure 6 Filter It checks whether the filter functions normally 8 3 2 Testing Motors and Recorder Printer At the Self test screen press F1 to select the Machine group as Figure 8 21 shows The following test items will be displayed on the right of the screen Press T or J to select the desired item and press ENTER to conduct the test Auto Hematology Analyzer Operation Manual V1 0 8 19 Maintenance Service Ready 00 33 O Tubing Result Machine O Valve O Circuit Rotation motor Syringe motor Elevator motor Print Reagents for gt 99 Counts MENU F1 Select test group f Select test ENTER Execute Figure 8 21 Testing mechanic parts 1 Syringe motor The syringe motor controls the aspiration volume This test checks whether the motor functions normally 2 Rotation motor The rotation motor rotates the sample probe inside the analyzer This test checks whether the motor functions no
165. s results this analyzer needs to work in a normal status Be sure to run the Self test items regularly to check the status of this analyzer When this analyzer gives alarms for clogging you can perform the Flush aperture or Zap aperture procedure or press F2 at the Count screen to unclog the system If you see other error messages see Chapter 9 Troubleshooting for solutions Auto Hematology Analyzer Operation Manual V1 0 8 2 System Maintenance Maintenance Press MENU to enter the system menu and press the appropriate arrow keys T JL to move the cursor to Service gt Maintenance as Figure 8 1 shows Count Whole Blood All Ready 23 57 Review Quality control gt Status Setup gt Self test Log Calibration gt Config Shutdown Print 100 200 300 fL Reagents for gt 99 Counts MENU T 1 lt 7 Select sub menu ENTER Enter sub menu Figure 8 1 Entering maintenance screen Press ENTER to enter the Maintenance screen as Figure 8 2 shows Service Ready Rinse prime Lyse prime Zap aperture Flush aperture Probe cleanser cleaning E Z cleanser cleaning Lyse test Clean bath Drain bath Drain tubing Clean wipe block Prepare to ship Prime the tubing with diluent Reagents for gt 99 Counts 23 57 MENU T 1 lt 7 Select service item ENTER Execute Fi
166. s trend represents Mean the mean value of the saved results Diff standard deviation of the saved analysis results CV Coefficient of Variation If the selected samples are less than 3 the three indices will all be 0 If the analysis result of certain parameter is invalid the three indices will also be invalid Under these two circumstances the three values on the left of the trends are the parameter s means and expected ranges set by the user see Chapter 7 2 6 gt x Mean n l Diff _ Diff Mean CV x 100 where n represents how many sample results are selected and X is the result of the in analysis 6 Every point in the graph is interpreted as follows The darkened square W that falls between the upper dash line and the lower dash line is within the expected range Otherwise it is not The blank square represents the sample analysis either ran into errors or the result is out of the display range 6 18 Auto Hematology Analyzer Operation Manual V1 00 Setup Chapter 7 Setup This BC 2800 Auto Hematology Analyzer is already set when delivered to you by the customer service engineers of Mindray or the distributor But you can still change the settings according to your own needs Note that you need the administrator password 2826 to change some of the settings 7 1 Password This analyzer divides the users into two categories common users and adminis
167. setting item F1 Select setting group Figure 7 15 Setting parity Press T or J to move the cursor to Odd Even or None and press ENTER to Auto Hematology Analyzer Operation Manual V1 0 7 11 Setup confirm the selection When you are done setting the parity check press T or J to move the cursor to other printing settings you want to change 3 Enabling or disabling Handshake If the Handshake function is enabled this analyzer send a handshake signal to the external computer that the data is to be sent to and waits for the response If the computer does not respond this analyzer aborts the transmission and gives an alarm for the transmission error If the Handshake function is disabled this analyzer transmits the data to the external computer regardless of the response This function is disabled by default Follow the steps given below to enable or disable it Atthe Setup screen press F1 to select the Print amp comm group Press T or 1 to move the cursor to the combo box right of Handshake in the Transmit field Press ENTER to display the pull down menu as Figure 7 16shows Setup Settings Ready 23 41 Reagent Print amp comm Date amp time Gain Count Ref range Other Print Transmit Device Farmat Auta Recorder Format 1 Off Baud 9600 Parity
168. smit them to the external computer as instructed by Chapter 6 2 4 A 3 3 Transmission at QC Table Screen Transmit the results as instructed by Chapter 4 1 5 A 4 Transmission Date Format A 4 1 Description A 4 1 1 Symbols Table A 1 Symbols ENQ 0x05 STX 0x02 EOT 0x04 EOF 0x1A ETX 0x03 ACK 0x06 NACK 0x15 A 0x41 B 0x42 ou 0x43 nae 0x30 0x39 ne Ox2A A 4 1 2 Programming If the Handshake is off BC 2800 will transmit the body of the text without acknowledging the presence of an external computer If the Handshake is on BC 2800 will communicate with the external computer in following A 2 Auto Hematology Analyzer Operation Manual V1 0 procedures Communication 1 BC 2800 sends an ENQ 05 Hex then waits up to 4 seconds for the external computer to respond If the external computer does not respond then one more ENQ 05 Hex is tried before giving up 2 The external computer must respond by sending an ACK 06 Hex If any other response is received another ENQ 05 Hex will be sent by the analyzer a maximum of only two ENQ 05 Hex will be sent 3 The analyzer then sends Body of text EOT 04 Hex ETX 03 Hex 4 Disconnection A BC 2800 sends an ETX 03 Hex then waits 4 seconds for the external computer to respond If no response is received one more ETX 03 Hex is sent BC 2800 waits 4 seconds before giving up and gives alarm of communication error B
169. ss lt or to move the cursor to No and press ENTER if you want to cancel the deletion 4 1 2 Selecting a QC File This analyzer provides 9 QC files for the user to store the QC settings and results Press F1 to select the desired QC file Each QC file can save the results of maximum 31 QC analyses When the file is full the result of the newest run automatically covers that of the oldest After selecting the QC file press F2 to select the Whole Blood or Prediluted mode 4 1 3 Editing QC Settings Before editing the QC settings make sure the QC file you have selected is empty If not you must follow either of the two ways given below to delete the existing results first 1 Atthe Controls screen press DEL to delete the existing results one by one Or 2 Go to the QC Table screen to delete all the existing results once and for all see section 4 1 5 2 QC Table for detailed steps When you are sure the selected QC file is empty press F3 to enter the QC Edit screen as Figure 4 4 shows Follow the instructions given below to edit the lot number and expiration Auto Hematology Analyzer Operation Manual V1 0 4 3 Quality Control date for the control material to be used and to assign the mean and expected ranges to the parameters to be included in the upcoming QC analysis If you press DEL at this screen you will delete all the entered data and the system will automatically set the current date as the
170. ss 1 to move the cursor to the edit box right of Bed No Press or gt to move the cursor within the box and press PgUp or PgDn or the numeric keys of an external keyboard to enter the digit at the position where the cursor is located When you are done entering the chart number press T or J to move the cursor to the next search condition to be specified To specify the period of time you may Press T or J to move the cursor to the check box left of Date Press ENTER to tick the box Press T or J to move the cursor to the combo box right of Start and press PgUp or PgDn to enter the start date in the YYYY MM DD format Press f or J to move the cursor to the combo box right of End and press PgUp or PgDn to enter the end date in the YYYY MM DD format When you are done specifying the period of time press T or 4 to move the cursor to the next search condition to be specified See Figure 6 19 for what the screen looks like if all conditions are selected Review Ready 20 55 Name Gender Dept ID ooooo00 Bed No 00000000 Chart No 00000000 Date Start 12 1 Reagents for gt 99 Counts MENU Figure 6 19 All conditions are selected for the search 8 To exit th
171. ssed by pressing ENTER Totally 30 items can be saved in the pull down menu and you may press f or to move the cursor to the interested item and press ENTER to select it C When you are done entering the name press f or 1 to move the cursor to the next field to be edited 11 When you are done entering the patient information you may A Move the cursor to Yes and press ENTER to save the changes and exit to the Count screen or B Move the cursor to No and press ENTER to exit to the Count screen without saving the changes Auto Hematology Analyzer Operation Manual V1 0 3 13 Sample Analysis 3 4 3 Batch Edit Mode If your analyzer is configured as Batch edit you can continuously enter information of a batch of samples Follow the steps given below to do so 1 Atthe Count screen press F4 to enter the Batch edit screen as Figure 3 8 shows Whole Blood All Batch edit Loc Total 0 0 ID A ID 00000001 Gender z Time Name i k Mode Age 000 Years oo Months foo Days pO fL Chart No 90000000 Bed No 00000000 Name Dept m Gender Sender Age Tester 0 fL Ne Checker ID 1 Next Prev Save Delete Mode 1 mm PCT 0 199 0 gt Se 10 15720256L Reagents for gt 99 Counts gt Move cursor
172. stogram F1 Goto F2 Select F3 Transmit F4 Search Figure 6 11 Five samples selected Review Ready 20 47 Reagents for gt 99 Counts MENU f Searched Histogram F1 Goto F2 Select F3 Transmit F4 Search Figure 6 12 Five samples de selected Example 2 You can also select or de select the results of multiple discontinued ranges If you want to select the sample results from Location 1 to Location 5 and from Location 7 to Location 8 in Figure 6 12 follow the steps given below to do so Follow the steps 1 5 introduced in Example 1 to select or de select the sample results from Location 1 to Location 5 Press T or J to move the cursor back to From and follow the steps 1 5 introduced in Example 1 to select or de select the sample results from Location 7 to Location 8 Press ft or to move the cursor to Exit and press ENTER to return to the Review screen to view the selected or de selected sample results as Figure 6 13 or Figure 6 14 shows Auto Hematology Analyzer Operation Manual V1 0 6 9 Sample Review Review Ready 20 48 Reagents for gt 99 Caunts MENU Searched Histogram F1 Goto F2 Select F3 Transmit F4 Search Figure 6 13 Discontinued samples selected Review Ready 20 49 Reagents for
173. sults In the Table mode you can select the results of several samples for such operations as deletion transmission and the like 1 To select de select single result Press lt or gt to move the cursor to the desired sample result Press ENTER to select or de select that result The selected result will be marked above its ID as the sample 117 shown in Figure 6 6 The disappears once the sample result is de selected as the sample 117 shown in Figure 6 7 Review Ready 20 41 Reagents for gt 99 Counts MENU 1 Searched Histogram F1 Goto F2 Select F3 Transmit F4 Search Figure 6 7 De select a sample 6 6 Auto Hematology Analyzer Operation Manual v1 00 2 To select de select multiple results Sample Review Example 1 Assuming you want to select or de select the sample results from Location 1 to Location 5 ID 118 114 in Figure 6 7 follow the steps given below to do so Press F2 to enter the Select screen as Figure 6 8 shows Ready 20 41 Location From 00001 To 00001 Select 8 De Select Reagents for gt 99 Counts Figure 6 8 Select screen To enter the start location 00001 press 1 or J to move the cursor to the edit box right of From press lt or gt to move the cursor within the box
174. t should be decreased 0 50 100 150 200 250 300 fL Figure 7 24 Too large WBC gain Follow the steps given below to set the WBC gain Obtain the administrator authority as instructed in Chapter7 1 1 Atthe Setup screen press F1 to select the Gain group Press T or J to move the cursor to the edit box right of WBC WB or WBC PB in the Gain field Press PgUp or PgDn to enter the desired gain When you are done entering the gain press T or 1 to move the cursor to other settings you want to change 7 2 4 2 Setting RBC gain When the difference between the actual MCV analysis result and the expected result exceeds 6 you need to change the RBC gain For example assuming the expected MCV result is 90 0fL while the actual analysis result is 82 0fL then Bape ieeMey 90 0 1 098 ActualMCV 82 0 Auto Hematology Analyzer Operation Manual V1 00 7 17 Setup You should adjust the RBC gain to 109 8 as close as possible Follow the steps given below to do so Obtain the administrator authority as instructed by Chapter 7 1 1 At the Setup screen press F1 to select the Gain group Press Tt or J to move the cursor to the edit box right of RBC in the Gain field Press PgUp or PgDn to enter the desired gain When you are done entering the gain press T or to move the cursor to other settings you want to change 7 2 4 3 Setting HGB gain You may adjust the HGB gain to change the H
175. ted ground line Note that it is imperative that this analyzer be grounded Be sure to connect the Equipotentiality at the back of the analyzer to a dedicated ground line Note Make sure the input power meets the requirements and an appropriate fuse is installed on it 2 2 Auto Hematology Analyzer Operation Manual V1 00 Installation 2 3 Installation 2 3 1 Tubing Connection Biohazard Consider all materials specimens reagents controls calibrators or components that contain or have contacted human blood as being potentially infectious Wear standard laboratory attire glove and follow safe laboratory procedures when handling any material in the laboratory 2 3 1 1 Connecting the diluent container Take out the diluent inlet tubing with a green connector from the accessory kit Take out the diluent container in which there should be enough diluent Insert the tubing end that has no connector into the diluent container as Figure 2 1 shows and turn the container cover clockwise until secure Connect the tubing connector to the DILUENT inlet green on the back of BC 2800 and turn it clockwise until secure Green tubing connector Figure 2 1 Diluent container Do the following steps when the installation is done 1 Enter the diluent volume as instructed by section 7 2 1 1 Setting remaining volumes for reagents 2 Enter the expiration date as instructed by section 7 2 1 3 Setting expiration dates of rea
176. ted range specified by the data sheet of the calibrator or control If not contact Mindray customer service department 5 22 Auto Hematology Analyzer Operation Manual V1 0 Sample Review Chapter 6 Sample Review This analyzer automatically saves the analysis results You can review the saved results in two modes histogram and table Auto Hematology Analyzer Operation Manual V1 0 6 1 Sample Review 6 1 Histogram Mode Press MENU to enter the system menu Press the appropriate arrow keys T J to move the cursor to Review as Figure 6 1 shows Ready 20 36 Whole Blood All 100 200 Quality control Setup Service Calibration gt Shutdown Reagents for gt 99 Counts MENU 1 1 7 Select sub menu ENTER Enter sub menu Figure 6 1 Entering Review screen Press ENTER to enter the Review screen as Figure 6 2 shows Review Ready 20 37 ID 118 01 200 300 fL 10 L Reagents for gt 99 Counts MENU 1 Searched Table F1 Goto F2 Edit gt Previous next sample Figure 6 2 Review screen This analyzer saves analysis results to two databases the Sample database which stores the sample analysis results and the Searched database which only stores the sample results matching the search conditions see Chapter 6 2 5 Search for detailed information To 6 2 Auto Hemato
177. that when you enable the Autoprint function make sure there is enough printing paper in the recorder or printer 3 6 1 5 Recount If the system detects clog or bubbles during the analysis or you press F5 a dialog box will pop up to ask you whether you want to have a re count analyze this sample again as Figure 3 11 shows Count Whole Blood Alll Ready 12 36 Current sample WBC 9 1 x 10 L Lymph 3 7 x 10 L i Mi d 0 7 xao WI poe iss Gran a7x ior Bl Time 01 24 2004 13 55 Lymph H 41 0 i Mid 8 0 WB All Mode AYESA Gran 51 0 Note Name Re count Gender Yes ie t No Next sample RDW CV 13 6 i RDW SD 40 2 fL P i ID 119 PLT 222 x 10 L i MPY 9 0 fL i Mode WB All PDW 157 PCT 0 199 0 65 10 15 20 25 fL Reagents for gt 99 Counts MENU Figure 3 11 Re count dialog box If you want to re analyze this sample move the cursor to Yes and press ENTER to enter the Recount screen otherwise move the cursor to No and press ENTER This Recount screen is similar to the Count screen except the lower sub area of the Sample Information area is tiled Recount as opposed to Next sample The sample ID remains unchanged Follow the previously introduced procedure to re analyze the sample in question The new result will overwrite the old result while the sample information keeps unchanged 3 6 1 6 Saving Analysis Results This analyzer automatically saves the ana
178. trators Some of the settings such as gain parameter units count time and etc can only be changed by the administrators 7 1 1 Obtaining Administrator Authority 1 Press MENU to enter the system menu and press the appropriate arrow keys T l i to Setup Password as Figure 7 1 shows Whole Blood All Current sample WBC 9 1 x 10 L Lymph 3 7 x 10 L Mi ct 0 7 x 10 L Wh ID 3118 Grane 47x tol Bl Time 01 24 2004 13 55 Lymph H 41 0 i i Mid 8 0 Mode WB All 0 100 200 300 fL Fo Gran 51 0 l HGB 124 g L Name RBC 4 08 x 1012 L HOT L 36 0 Count V 88 3 fL i i 4 30 3 pg 0 50 150 250 fL Review HC 344 g L F WY CY 13 6 aua ity control gt 12 fL setup M22 x 10 L Service Settings 3 0 fL gt F 1 7 i Calshratinn gt F 0 199 0 5 10 15 20 25 fL Shutdown Reagents for gt 99 Counts MENU T 1 7 Select sub menu ENTER Enter sub menu Figure 7 1 Entering the password screen 2 Press ENTER to enter the Password screen as Figure 7 2 shows The displayed password is the default common user password set automatically by this analyzer Auto Hematology Analyzer Operation Manual V1 00 7 1 Setup Setup Ready 21 05 Password Reagents for gt 99 Counts MENU lt 7 Move the cursor Figure 7 2 Conmon user password 3 Press lt or to move the cursor within the edit box right of Password Press PgUp o
179. ts and then press F5 to enter the Special Functions screen as Figure 6 21 shows If you want to print out the displayed data press PRINT If you want to acquire help information press HELP If you want to exit the screen press MENU Auto Hematology Analyzer Operation Manual V1 00 6 15 Sample Review Review Ready 21 02 Reprodu 2 1 1 5 3 a 6 3 3 3 4 co NMORPHONH HOH HOO wMH NOoOH 1 5 1 o n Reagents for gt 99 Caunts MENU Figure 6 21 Special functions screen open to all users Two special functions are included in this screen Reprodu and Trend The former is open to all users as Figure 6 21 shows while the latter to administrators only as Figure6 22 shows Review Ready 21 02 Reprodu O Trend WBC Lymph Mid Gran Lymph Mid Gran HGB RBC HCT MCV MCH MCHC RDW CY RDVV SD PLT MPV PDW Soooco0o0oo0g ooog oO ooc00c00ce000 oO O n WO NONH BOSS OO U HNO cCoOCO cOmMmMo000 c0oc00000 200000 Reagents for gt 99 Counts MENU F1 Select the desired function Figure 6 22 Special functions screen open to only administrators 6 2 7 1 Reproducibility See Figure 6 22 for the Special Functions screen open to all users The screen cons
180. ts of 18 keys as Figure 1 9 shows Figure 1 9 Keypad E1 2 3 4 65 Eee Ceao gu GES E O Se se A PS 2 keyboard can be connected to the PS 2 interface of this analyzer and it is useful when you have to edit complicated information See Table 1 6 for the major functions of the keys Table 1 6 Key functions START i Keypad Keyboard Functions key MENU Esc Enters exits the system menu PRINT P or p Prints by the recorder or printer HELP H or h Displays help information DEL Del or Delete Deletes data or task DILUENT Enters the Add diluent screen Switches to another analysis mode MODE Ctrl A f works only in the Count screen ENTER Enter Confirms a certain operation Switches to another screen enters PgUp and PgDn PgUp or PgDn digits NN MN Moves the cursor to a certain Auto Hematology Analyzer Operation Manual V1 00 1 19 Introduction SIC SII position F1 F2 F3 F4 F1 F2 F3 F4 Function keys F5 F5 Other keys Other functions START Starts aspiration 1 6 4 Screen Saver To extend the service life of the LCD this analyzer will enter the screen saver if no any operation was done in the past 10 minutes When it happens the LCD will be dark and the power indicator will be flickering You can press any key to resume the dis
181. unt t WBC Second RBC Second Reagents for gt 99 Counts MENU 7 Move cursor f Select setting item F1 Select setting group Figure 7 28 Selecting parameter unit Press T or J to move the cursor to the desired unit and press ENTER to confirm the selection When you are done selecting units press 1 or J to move the cursor to other settings you want to change 7 2 5 2 Setting count time If the WBC or RBC count time is inappropriately set the system may give false alarms for clogs or bubbles When this happens follow the steps given below to change the WBC or RBC count time Refer to the actual count time see Chapter 8 3 1 for details when editing the settings here Obtain the administrator authority as instructed in Chapter7 1 1 At the Setup screen press F1 to select the Count group Press T or J to move the cursor to the edit box right of WBC or RBC in the Count time field Press PgUp or PgDn to enter the desired time When you are done entering the count time press T or J to move the cursor to other settings you want to change 7 2 5 3 Exiting setting parameter units and count time When you are done changing all the printing and transmission settings you want to change you may press F1 to select other setting group you want to change or press MENU and a dialog box will pop up to remind you to save the changes
182. up Figure 7 20 Setting date format Press T or J to move the cursor to YYYY MM DD MM DD YYYY or DD MM YYYY and press ENTER to confirm the selection When you are done enabling or disabling the auto transmission press T or 4 to move the cursor to other settings you want to change 7 2 3 3 Setting time Follow the steps given below to enter the system time At the Setup screen press F1 to select the Date amp Time group Press f or J to move the cursor to the edit box right of Hour minute or second in the Time field Press PgUp or PgDn to enter the desired time When you are done entering the system time press T or J to move the cursor to other settings you want to change 7 2 3 4 Exiting setting date and time When you are done changing all the printing and transmission settings you want to change Auto Hematology Analyzer Operation Manual V1 0 7 15 Setup you may press F1 to select other setting group you want to change or press MENU and a dialog box will pop up to remind you to save the changes as Figure 21 shows Press or gt to move the cursor to Yes and press ENTER to save the changes and exit to the system menu or move the cursor to No and press ENTER to exit to the system menu without saving the changes Setup Settings Ready 23 43 oo0oo0o00o0o0 Reagent Print amp comm Date amp time Gain Count Ref range Other
183. ve the new factors press gt to move the cursor to Yes and press ENTER to save the factors to the Manual calibration screen in another calibration mode Otherwise move the cursor to No and press ENTER to switch to the Manual calibration screen in another calibration mode without saving the new factors Calibration Lot No Exp Date 01 27 2 Ready Whole Blood O Prediluted Para Mean 1 2 3 4 Factor WBC 4 5 43 4 1 Save new Yes factors 2 73 106 3 Reagents for gt 99 Counts MENU Press MENU and a dialog box will pop up to ask you to save the new factors as Figure5 14 shows To save the new factors press lt gt to move the cursor to Yes and press ENTER to save the factors to the system Otherwise move the cursor to No and press ENTER to switch to the system menu without saving the new factors Figure5 14 Saving changes 5 12 Auto Hematology Analyzer Operation Manual V1 00 18 40 Calibration 6 Other operations f calibration data calibration results CV or new factors exist when you press F2 a dialog box will pop up to warn you as Figure5 15 shows Press ENTER to return to the Auto calibration screen Calibration Ready 18 35 Lot No Exp Date 01 27 2 Whole Blood O Prediluted Para Mean 3 4 Factor WBC 4 5 Results prese
184. w safe laboratory procedures when handling any material in the laboratory Probe Hazard The probe is sharp and may contain biohazardous materials including controls and calibrators Avoid any unnecessary contact with the probe and probe area NOTE Indicate to the operator information of importance in the procedure to be performed This heading is also used to indicate specific sample handling techniques that are helpful in operating the instrument CAUTION Indicate to the operator information of importance that could result in damage to the instrument or affect the test due to improper operation if these instructions are not followed WARNING Indicate to the operator information regarding potential hazards that may cause personal harm to the instructions are not operator if these followed Auto Hematology Analyzer Operation Manual v1 00 1 A oo E eo 2 on ee OS ee p ao A amp D aA gl A l nD aA an an l m eS A ing A nD ea es oS al oC DC AD i A db 2p a2 a D Io a D a D A D a D a D A D A D A D if i O10 I i V o 20 WARNING To avoid electrical shock be sure to disconnect the power supply before maintaining this device BIOHAZARD Consider waste that contain or have contacted human blood as being potentiall
185. will pop up to remind the user to perform the Probe cleanser cleaning procedure If you want to do so move the cursor to Yes and press ENTER otherwise move the cursor to No and press ENTER 8 1 7 E Z Cleanser Cleaning Biohazard D 4 Wear standard laboratory attire glove and follow safe laboratory procedures when handling any material in the laboratory You can use the E Z cleanser an enzyme based isotonic cleaning solution and wetting agent to clean the tubing and bath by performing the E Z cleanser cleaning procedure 8 8 Auto Hematology Analyzer Operation Manual V1 00 Maintenance Follow the steps given below to perform the procedure At the Maintenance screen press the appropriate arrow keys T 4 to move the cursor to E Z cleanser cleaning Present the cleanser to the probe and press ENTER to aspirate the cleanser Remove the cleanser after the probe has risen up This analyzer will automatically soak the bath and tubing with the aspirated cleanser The soaking process will last about 10 minutes and you may press ENTER to stop the process any time Note that a shortened soaking process may not present such a good result as a complete one When the soaking is done press ENTER to flush the bath and tubing and the screen will return to the initial state If you analyzer has been running continuously for 24 hours the dialog box will pop up to remind the user to perform the Probe cle
186. y WARNING Handle and dispose of the waste according to acceptable laboratory local state and national standards 3 28 Auto Hematology Analyzer Operation Manual V1 0 Quality Control Chapter 4 Quality Control After being used for a certain period of time the hematology analyzer is subject to errors which may lead to misleading or unreliable analysis results To maintain the accuracy and precision of this analyzer Mindray recommends that the quality control QC program be run daily with controls of low normal and high levels provided the user is familiar with the principle and approach of quality control This analyzer provides two QC programs QC with controls and X B analysis Auto Hematology Analyzer Operation Manual V1 0 4 1 Quality Control 4 1 QC with Controls BC 2800 provides 9 QC files and can include maximum 12 parameters to this QC program 4 1 1 Entering the Controls Screen Press MENU to enter the system menu Press the appropriate arrow keys f lt to move the cursor to the QC Controls as Figure 4 1 shows Current sample WBC 9 1 x 10 L Lymph 3 7 x 10 L ig Mid 0 7 x10 W CASAS Grant 47x10 Time 01 24 2004 13 55 Lymph H 41 0 Mid 8 0 Mode WB All SR Gran 51 0 HGB 124 g L Name RBC Count 150 Review Quality control Setup gt Service gt Calibration gt Shutdown MENU ect sub menu ENTER E
187. y infectious Wear laboratory attire glove and follow safe laboratory procedures when handling any material in the laboratory 2 Auto Hematology Analyzer Operation Manualg v1 00 0 30 Equipotentiality WARNING To avoid being injured do not put hand under the motor when the machine is running Auto Hematology Analyzer Operation Manual v1 00 0 50 O 50 andi 60 A WARNING To avoid electrical shock be sure to disconnect the power supply before maintaining this device LOT FR IN VITRO DAGNSITC USE PATCH QIE UE BY in inal PPNA 4 Auto Hematology Analyzer Operation Manual v1 00 AUTHOR SED REPRESENIATI VE I NTHE EURCPEANCOMMIN TY IRA NOR Auto Hematology Analyzer Operation Manual v1 00 5 AUTHOR SED REPRESENIATI VE I NTHE EURCPEANCOMMIN TY IRA NOR Auto Hematology Analyzer Operation Manual v1 00 5 Content Content Chapter 1 TL TIEFOGUCE FOU caesen cain Rit ona aur eee ee awa 1 1 Tt FOR GWOW ocn vac dhe stan etapa Ada catien oad eda ban Seed shea dena eee te ee 1 1 12 General 2 2 neiseve idee av hil nila uaa eine ceded lia outlays 1 2 13 System Introduction ccciad sau aai vi cident Ma erties Geen aes 1 3 1 4 Principles of Operation cc cece ccc cece eeeeeeeeeae
188. ys in which analysis count mode the next sample is to be analyzed In the case of Figure 3 2 the next sample is to be analyzed in the WB AlIl mode see section 3 6 1 Selecting Analysis Mode for detailed information 3 3 3 System Status Area The System Status area displays whether this analyzer is ready for the next analysis When it displays Ready as in Figure 3 2 it means this analyzer is ready and you can proceed to analyze the next sample When it displays Waiting it means this analyzer is still preparing for the next analysis When it displays Running it means this analyzer is analyzing a sample 3 3 4 System Time Area The System Time area displays the system time in the 24 hour format 3 3 5 Sample Information Area The Sample Information area has two sub areas the upper titled Current sample and the lower Next sample The Current sample refers to the sample whose analysis result is displayed on the Count screen Its sample ID time of analysis analysis mode and patient information name gender and age are respectively displayed in the ID Time Mode Name Gender and Age fields of the Current sample sub area The Next Sample refers to the sample to be analyzed next Its sample ID and analysis mode are respectively displayed in the D and Mode fields of the Next sample part 3 3 6 Analysis Result Area The Analysis Result area displays the analysis result including histograms of the current sample 3 3 7 Erro

MINDRAY BC-2800 Hematology Analyzer Operaters

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