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Agilent 1200 Infinity Series Diode Array Detectors

1. Instant Pilot Solvent cabinet Detector Column compartment Autosampler Pump Figure 9 Recommended stack configuration for 1290 Infinity with binary pump front view Agilent 1200 Infinity Series DAD User Manual 35 3 Installing the Module Optimizing the Stack Configuration LAN to LC ChemStation INAV WINN CAN Bus cable to Instant Pilot Analog detector signal optional AC Power CAN Bus cable Figure 10 Recommended stack configuration 1290 Infinity with binary pump rear view 36 Agilent 1200 Infinity Series DAD User Manual Installing the Module 3 Optimizing the Stack Configuration Two Stack Configuration Two Stack Configuration for Agilent 1260 Infinity LC To avoid excessive height of the stack when the autosampler thermostat is added to the system it is recommended to form two stacks Some users prefer the lower height of this arrangement even without the autosampler thermostat A slightly longer capillary is required between the pump and autosampler See Figure 11 on page 37 and Figure 12 on page 38 Instant Pilot f Irre Detector Column compartment HM A Solvent cabinet Degasser optional Autosampler ALS Fraction collector Thermostat for the ALS Fraction collector optional Figure 11 Recommended Two Stack C
2. Notices Agilent Technologies Inc 2010 2011 2012 2013 No part of this manual may be reproduced in any form or by any means including elec tronic storage and retrieval or translation into a foreign language without prior agree ment and written consent from Agilent Technologies Inc as governed by United States and international copyright laws Manual Part Number 64212 90013 Edition 02 2013 Printed in Germany Agilent Technologies Hewlett Packard Strasse 8 76337 Waldbronn This product may be used as a com ponent of an in vitro diagnostic sys tem if the system is registered with the appropriate authorities and com plies with the relevant regulations Otherwise it is intended only for gen eral laboratory use Warranty The material contained in this docu ment is provided as is and is sub ject to being changed without notice in future editions Further to the max imum extent permitted by applicable law Agilent disclaims all warranties either express or implied with regard to this manual and any information contained herein including but not limited to the implied warranties of merchantability and fitness for a par ticular purpose Agilent shall not be liable for errors or for incidental or consequential damages in connection with the furnishing use or perfor mance of this document or of any information contained herein Should Agilent and the user have a separate written agreeme
3. The Agilent ChemStation B 04 02 and above may not include any maintenance test functions Screenshots used within these procedures are based on the Agilent Lab Advisor software Agilent 1200 Infinity Series DAD User Manual 113 Troubleshooting and Diagnostics Agilent Lab Advisor Software 114 The Agilent Lab Advisor software is a standalone product that can be used with or without data system Agilent Lab Advisor software helps to manage the lab for high quality chromatographic results and can monitor in real time a single Agilent LC or all the Agilent GCs and LCs configured on the lab intranet Agilent Lab Advisor software provides diagnostic capabilities for all Agilent 1200 Infinity Series modules This includes diagnostic capabilities calibration procedures and maintenance routines for all the maintenance routines The Agilent Lab Advisor software also allows users to monitor the status of their LC instruments The Early Maintenance Feedback EMF feature helps to carry out preventive maintenance In addition users can generate a status report for each individual LC instrument The tests and diagnostic features as provided by the Agilent Lab Advisor software may differ from the descriptions in this manual For details refer to the Agilent Lab Advisor software help files The Instrument Utilities is a basic version of the Lab Advisor with limited functionality required for installation use and maintenance No advanced rep
4. 2 Connect the instrument s LAN interface see Figure 56 on page 224 to the PC network card using a crossover network cable point to point or a hub or switch using a standard LAN cable LAN interface MAC label ts Pent HIMNAMN EE mectemmmemenn Figure 56 Location of LAN interfaces and MAC label 224 Agilent 1200 Infinity Series DAD User Manual LAN Configuration 13 TCP IP parameter configuration To operate properly in a network environment the LAN interface must be configured with valid TCP IP network parameters These parameters are e IP address e Subnet Mask Default Gateway The TCP IP parameters can be configured by the following methods e by automatically requesting the parameters from a network based BOOTP Server using the so called Bootstrap Protocol by automatically requesting the parameters from a network based DHCP Server using the so called Dynamic Host Configuration Protocol This mode requires a LAN onboard Module or a G1369C LAN Interface card see Setup DHCP on page 232 e by manually setting the parameters using Telnet e by manually setting the parameters using the Instant Pilot G4208A The LAN interface differentiates between several initialization modes The initialization mode short form init mode defines how to determine the active TCP IP parameters after power on The parameters may be derived from a Bootp cycle non volatile memor
5. 150 bar 2175 psi Maximum Incidential Pressure MIP 2 pH range 1 0 12 5 solvent dependent available as standard and bio inert versions Cartridge type equipped with RFID tags that holds cell typical information B 02 11 or above B 01 03 SP4 or above 1 conditions Maximum Operating Pressure MOP The maximum pressure at which the system can operate continuously under normal 2 Maximum Incidential Pressure MIP The maximum pressure which the system can experience during a short time Agilent 1200 Infinity Series DAD User Manual 27 2 28 Site Requirements and Specifications Specification Conditions ASTM Standard Practice for Variable Wavelength Photometric Detectors Used in Liquid Chromatography Reference conditions Wavelength 230 nm 4 nm with Reference Wavelength 360 nm 100 nm Slitwidth 4 nm TC 2 s or with RT 2 2 TC ASTM Max Light Cartridge Cell 10 mm V o 1 0 uL G4212 60008 with flow of 0 5 mL min LC grade water or Max Light Cartridge Test Cell G4212 60011 Linearity Linearity is measured with caffeine at 265 nm 4 nm with slit width 4 nm and TC 1 s or with RT 2 s with Max Light Cartridge Cell 10 mm V o 1 0 uL G4212 60008 gt 2 0 AU 5 typical 2 5 AU 5 The specifications are based on the standard RFID tag lamp 5190 0917 and may be not achieved when other lamp types or aged lamps are used ASTM drift tests require a temperature change below 2
6. Appendix 14 General Safety Information The following general safety precautions must be observed during all phases of operation service and repair of this instrument Failure to comply with these precautions or with specific warnings elsewhere in this manual violates safety standards of design manufacture and intended use of the instrument Agilent Technologies assumes no liability for the customer s failure to comply with these requirements WARNING Ensure the proper usage of the equipment The protection provided by the equipment may be impaired gt The operator of this instrument is advised to use the equipment in a manner as specified in this manual Safety Standards This is a Safety Class I instrument provided with terminal for protective earthing and has been manufactured and tested according to international safety standards Agilent 1200 Infinity Series DAD User Manual 263 14 Appendix Operation Before applying power comply with the installation section Additionally the following must be observed Do not remove instrument covers when operating Before the instrument is switched on all protective earth terminals extension cords auto transformers and devices connected to it must be connected to a protective earth via a ground socket Any interruption of the protective earth grounding will cause a potential shock hazard that could result in serious personal injury Whenever it is likely that the pr
7. Figure 46 Wavelength Calibration Results Agilent 1200 Infinity Series DAD User Manual 157 8 Test Functions and Calibration Wavelength Recalibration Fails Probable cause Suggested actions 1 Absorbing solvent or air bubble in Max Light Repeat calibration with Max Light Cartridge Cartridge Cell Test Cell and compare results 2 Dirty or contaminated Max Light Cartridge Ensure the Max Light Cartridge Cell is filled Cell with water e Recalibrate 3 Old UV lamp Exchange the UV lamp 4 Dirty or contaminated optical components Run the Cell Test If the test fails flush the flow cell See also Cleaning of Max Light Cartridge Cell on page 178 If the test fails with Max Light Cartridge Test Cell and new UV lamp the optical unit must be replaced 158 Agilent 1200 Infinity Series DAD User Manual Test Functions and Calibration 8 D A Converter DAC Test When Preparations The detector provides analog output of chromatographic signals for use with integrators chart recorders or data systems The analog signal is converted from the digital format by the digital analog converter DAC The DAC test is used to verify correct operation of the digital analog converter by applying a digital test signal to the DAC The DAC outputs an analog signal of approximately 50 mV if the zero offset of the analog output is set to the default value of 5 which can be plotted on an integrator A continuous square wave with
8. ULD Max Light Cartridge Cell 10 mm V o 0 6 pL G4212 60038 Max Light Cartridge Cell Bio inert 10 mm V o 1 0 uL G5615 60018 Max Light Cartridge Cell Bio inert 60 mm V o 4 0 uL G5615 60017 Max Light Cartridge Test Cell G4212 60011 Agilent Instant Pilot G4208A Agilent LabAdvisor Recorder integrator 100 mV or 1 V output range 0 001 2 AU one output Controller area network CAN RS 232C APG Remote ready start stop and shut down signals LAN Extensive support for troubleshooting and maintenance is provided by the Instant Pilot Agilent Lab Advisor and the Chromatography Data System Safety related features are leak detection safe leak handling leak output signal for shutdown of pumping system and low voltages in major maintenance areas Early maintenance feedback EMF for continuous tracking of instrument usage in terms of lamp burn time with user setable limits and feedback messages Electronic records of maintenance and errors Verification of wavelength accuracy with the emission lines of the deuterium lamp All materials recyclable Operating Pressure Mop 150 bar 2175 psi Maximum Incidential Pressure MIP 2 pH range 1 0 12 5 solvent dependent available as standard and bio inert versions Cartridge type equipped with RFID tags that holds cell typical information B 02 11 or above B 01 03 SP4 or above 1 conditions Maximum Operating Pressure MOP The maximum
9. 2 Defective connector or cable Please contact your Agilent service representative UV Lamp Current Error ID 7450 The UV lamp current is missing The processor continually monitors the anode current drawn by the lamp during operation If the anode current falls below the lower current limit the error message is generated Probable cause Suggested actions 1 Lamp disconnected Ensure the UV lamp connector is seated firmly 2 Defective UV lamp or non Agilent lamp Exchange the UV lamp 3 Defective detector main board Please contact your Agilent service representative 4 Defective power supply Please contact your Agilent service representative 126 Agilent 1200 Infinity Series DAD User Manual UV Lamp Voltage Error ID 7451 The UV lamp anode voltage is missing Error Information 7 The processor continually monitors the anode voltage across the lamp during operation If the anode voltage falls below the lower limit the error message is generated Probable cause 1 Defective UV lamp or non Agilent lamp 2 Defective detector main board 3 Defective power supply UV Ignition Failed Error ID 7452 The UV lamp failed to ignite Suggested actions Exchange the UV lamp Please contact your Agilent service representative Please contact your Agilent service representative The processor monitors the UV lamp current during the ignition cycle If the lamp current does not rise above the lower limit within 2 5 sec
10. Optimization Overview Table6 Optimization Overview Parameter Impact 1 Selection of flow cell peak resolution versus sensitivity Choose flow cell according to used column Choosing a Flow Cell on page 80 2 Connection of flow cell chromatographic resolution 3 Setting the peak width response time peak resolution versus sensitivity versus disk space Use peak width according Choosing a Flow Cell on page 80 as starting point Set the peak width close to the width of a narrow peak of interest in your chromatogram 4 Setting wavelength and bandwidth Sample wavelength Never miss a peak by the use of a browser wavelength like 250 nm with e sensitivity versus selectivity 100 nm bandwidth Select specific wavelength with reduced optical bandwidth if you need e sensitivity versus linearity selectivity e g 254 0 nm 4 nm and 360 0 nm 100 nm as reference wavelength Set the sample wavelength to a peak or valley to get best linearity in general select a valley to get best linearity for high concentrations Reference wavelength Select the reference wavelength with broad bandwidth 30 100 nm baseline drift due to RI effects wavelength range where your analytes have little or no absorbance e g sample at 254 nm reference at 320 nm Select the reference wavelength as near as possible to the UV range 5 Setting the slit width G4212A only Use 4 nm slit for normal applications spectr
11. Results Figure42 ASTM Drift and Noise Test Signals Agilent 1200 Infinity Series DAD User Manual 149 8 Test Functions and Calibration Test Failed ASTM Noise Test Evaluation Probable cause Suggested actions 1 Insufficient lamp warm up time Allow detector and UV lamp turned on for at least 2 hours 2 Absorbing solvent or air bubble in flow cell Ensure the flow cell is filled with water and free from air bubbles 3 Dirty or contaminated flow cell Flush flow cell e Clean the flow cell as described in Cleaning of Max Light Cartridge Cell on page 178 4 Old UV lamp Exchange the UV lamp 5 Environment not according to Improve environment specifications 150 Agilent 1200 Infinity Series DAD User Manual Test Functions and Calibration 8 Slit Test Slit Test G4212A The slit test verifies correct operation of the micromechanical slit During the test the slit is moved through all slit positions while the detector monitors the lamp intensity change When the slit position is changed the intensity drop move to smaller slit or intensity increase move to larger slit must be within a defined range If the intensity changes are outside the expected range the test fails When In case of problems Parts required Description 1 Max Light Cartridge Cell filled with water OR 1 Max Light Cartridge Test Cell Preparations Lamp must be on for at least 10 minutes When using a Max Light Cart
12. 33333333 20 10 min 2 0 z reponse tme 25 He Stogtime Pomme w may 4 m Agilent 1200 Infinity Series DAD User Manual 57 4 Using the Module General Method Settings Signals Signals Use Wave Band Reference Reference Signal length width Wavelength Bandwidth Signal A vi 254 0 40 gt v 3600 gt 1000 nm Signal B v 2100 40 gt v 3600 1000 nm Signal C v 2140 40 v 3600 gt 1000 nm Signal D v 2300 gt 40 v 3600 1000 nm Signal E v 2600 40 gt v 3600 gt 1000 nm Signal F nm Signal G nm Signal H nm Peakwidth Peakwidth gt 0 10 min 2 0 s response time 2 5 H2 lv lt 0 0016 min 0 016s response time 160 H2 gt 0 0016 min 0 03s response time 160 Hz gt 0 003 min 0 062 s response time 80 Hz J gt 0 006 min 0 12 s response time 40 Hz gt 0 012 min 0 25 s response time 20 H2 gt 0 025 min 0 5 s response time 10 H2 min gt 0 05 min 1 0 s response time 5 Hz gt 0 20 min 4 0 s response time 1 25 H2 gt 0 40 min 8 0 s response time 0 62 H2 58 gt 0 85 min 16 0 s response time 0 31 H2 Up to 8 individual signals can be set For each of the signals the wavelength and bandwidth can be set for sample and reference Limits Wavelength 190 0 to 640 0 nm in steps of 0 1 nm Bandwidth 1 0 to 400 0 nm in steps of 0 1 nm Setting an appropriate reference wavelength could improve the baseline behavior Alternatively use the 1 6 pL heat exchanger
13. Blue Shut down Low e oa NC 5 Pink Not Ls connected NC 6 Yellow Power on High 5 14 7 Red Ready High 1 8 Green Stop Low NC 9 Black Start request Low 13 15 Not connected Agilent Module to 3396 Series II 3395A Integrators Use the cable Agilent module to 3396A Series I integrators 03394 60600 and cut pin 5 on the integrator side Otherwise the integrator prints START not ready Agilent 1200 Infinity Series DAD User Manual Identifying Cables 11 Agilent Module to 3396 Series III 3395B Integrators p n 03396 61010 Pin 33XX Pin Agilent Signal Name Active module TTL 9 1 White Digital ground NC 2 Brown Prepare run Low 80 i 3 3 Gray Start Low z NC 4 Blue Shut down Low e an NC 5 Pink Not is connected NC 6 Yellow Power on High 14 7 Red Ready High 4 8 Green Stop Low NC 9 Black Start request Low 13 15 Not connected Agilent Module to Agilent 35900 A D Converters p n 5061 3378 Pin 35900 Pin Agilent Signal Name Active A D module TTL 1 White 1 White Digital ground 2 Brown 2 Brown Prepare run Low 3 Gray 3 Gray Start Low 4 Blue 4 Blue Shut down Low 5 Pink 5 Pink Not connected 6 Yellow 6 Yellow Power on High 7 Red 7 Red Ready High 8 Green 8 Green Stop Low 9 Black 9 Black Start request Low Agilent 1200 Infinity Series DAD User Manual 197 11 Identifying Cables Agilent Modu
14. C hour 3 6 F hour over one hour period Our published drift specification is based on these conditions Larger ambient temperature changes will result in larger drift Better drift performance depends on better control of the temperature fluctuations To realize the highest performance minimize the frequency and the amplitude of the temperature changes to below 1 C hour 1 8 F hour Turbulences around one minute or less can be ignored Performance tests should be done with a completely warmed up optical unit gt two hours ASTM measurements require that the detector should be turned on at least 24 h before start of testing Time Constant versus Response Time According to ASTM E1657 98 Standard Practice of Testing Variable Wavelength Photometric Detectors Used in Liquid Chromatography the time constant is converted to response time by multiplying by the factor 2 2 Agilent 1200 Infinity Series DAD User Manual Agilent 1200 Infinity Series DAD User Manual e Installing the Module J Unpacking the Module 30 Damage to the module Delivery Checklist 31 Detector Accessory Kit Contents 30 31 Optimizing the Stack Configuration 32 One Stack Configuration 33 Two Stack Configuration 37 Installing the Detector 41 Flow Connections to the Detector 44 Initial Recalibration 48 This chapter provides information on unpacking checking on completeness stack considerations and installation of the module 3 a
15. Optimizing Selectivity Quantifying Coeluting Peaks by Peak Suppression In chromatography two compounds may often elute together A conventional dual signal detector can only detect and quantify both compounds independently from each other if their spectra do not overlap However in most cases this is highly unlikely With a dual channel detector based on diode array technology quantifying two compounds is possible even when both compounds absorb over the whole wavelength range The procedure is called peak suppression or signal subtraction As an example the analysis of hydrochlorothiazide in the presence of caffeine is described If hydrochlorothiazide is analyzed in biological samples there is always a risk that caffeine is present which might interfere chromatographically with hydrochlorothiazide As the spectra in Figure 25 on page 96 shows hydrochlorothiazide is best detected at 222 nm where caffeine also shows significant absorbance It would therefore be impossible with a conventional variable wavelength detector to detect hydrochlorothiazide quantitatively when caffeine is present WL1 204 nm caffeine WL2 222 nm hydrochlorothiazide WL3 260 nm reference to suppress hydrochlorothiazide WL4 282 nm reference to suppress caffeine WL3 WL4 ia WLI WL2 Wavelength nm Figure 25 Wavelength Selection for Peak Suppression 96 Agilent 1200 Infinity Series DAD User Manual Optimizing the Detector 5
16. Ref 360 0 100 0 nm Signal E 1260 0 4 0 Ref 360 0 100 0 nm Signal F Signal G Signal H ji UV Lamp Required f Spectrum __190 0 nm 400 0 nm step 2 0 nm Slit Width fi Agilent 1200 Infinity Series DAD User Manual 71 4 Using the Module Main Screens of the Detector with Agilent Instant Pilot G4208A Maintenance DAD 1 Calibrate 2 Lamp Info o ErrorEvents m T entenance Entries 3 Caino DIETE DAD PRODI0007 Calibration Test Cell test No Pass Fail result 72 The Maintenance screen allows EMF setup e Maintenance calibrate cell lamp info logging of maintenance activities module identification blinking LED Firmware Updates can be done via the System Maintenance screen The Diagnose screen provides access to module specific tests Lamp Intensity Calibration gt Cell Agilent 1200 Infinity Series DAD User Manual Using the Module 4 Solvent Information Observe the following recommendations on the use of solvents e Follow recommendations for avoiding the growth of algae see pump manuals e Small particles can permanently block capillaries and valves Therefore always filter solvents through 0 4 um filters Avoid or minimize the use of solvents that may corrode parts in the flow path Consider specifications for the pH range given for different materials like flow cells valve materials etc and recommendations
17. about pH 0 1 at room temperature is about 13 um year At room temperature titanium is resistant to concentrations of about 5 sulfuric acid about pH 0 3 The addition of nitric acid to hydrochloric or sulfuric acids significantly reduces corrosion rates Titanium is subject to corrosion in anhydrous methanol which can be avoided by adding a small amount of water about 3 Slight corrosion is possible with ammonia gt 10 Fused silica Fused silica is inert against all common solvents and acids except hydrofluoric acid It is corroded by strong bases and should not be used above pH 12 at room temperature The corrosion of flow cell windows can negatively affect measurement results For a pH greater than 12 the use of flow cells with sapphire windows is recommended Agilent 1200 Infinity Series DAD User Manual Using the Module 4 Gold Gold is inert to all common HPLC solvents acids and bases within the specified pH range It can be corroded by complexing cyanides and concentrated acids like aqua regia a mixture of concentrated hydrochloric and nitric acid Zirconium Oxide Zirconium Oxide ZrO is inert to almost all common acids bases and solvents There are no documented incompatibilities for HPLC applications Platinum Iridium Platinum Iridium is inert to almost all common acids bases and solvents There are no documented incompatibilities for HPLC applications PTFE PTFE polytetrafluorethen is inert to al
18. change and you may click on the button 1 to Make Device Ready Turn device off standby turn on off the lamp Signal Information activated by button 1 shows actual values of all selected signals Signal name A B C Sample wavelength bandwidth Reference wavelength bandwidth Absorbance If more signals are activated the size of the detector GUI will change accordingly 53 4 54 Using the Module Setting up the Detector with Agilent ChemStation S 1290 Infinity DAD Idle M WL BW RefWL RefBW mAU A 250 0 100 off off 11 A right click into the Active Area will open a menu to Show the Control Interface special module settings Show the Method interface similar as via menu Instrument Setup Instrument Method Set Error Method Identify Module Status LED will blink Perform a Balance Switch the UV lamp on off same as click on button Make Device Ready Turn device off standby Module Status shows Run Ready Error state and Not Ready text or Error text Error Red Not ready yellow Ready green Pre run Post run purple Run blue Idle green Offline dark gray Standby light gray Agilent 1200 Infinity Series DAD User Manual Idle E 1290 Infinity DAD Idle B E Agilent 1200 Infinity Series DAD User Manual Using the Module 4 Setting up the Detector with Agilent ChemStation EMF Status shows Offline gray Ok No
19. completed for example immediately after changing a set point or while a self test procedure is running An error condition is indicated when the status indicator is red An error condition indicates the module has detected an internal problem which affects correct operation of the module Usually an error condition requires attention e g leak defective internal components An error condition always interrupts the analysis If the error occurs during analysis it is propagated within the LC system i e ared LED may indicate a problem of a different module Use the status display of your user interface for finding the root cause module of the error A blinking indicator indicates that the module is in resident mode e g during update of main firmware A fast blinking indicator indicates that the module is in a low level error mode In such a case try to re boot the module or try a cold start see Special Settings on page 219 Then try a firmware update see Replacing Module Firmware on page 183 If this does not help a main board replacement is required Agilent 1200 Infinity Series DAD User Manual Troubleshooting and Diagnostics 6 Available Tests vs User Interfaces Depending on the user interface the available tests and the screens reports may vary see chapter Test Functions and Calibrations e Preferred tool should be the Agilent Lab Advisor software see Agilent Lab Advisor Software on page 114
20. one start bit and one stop bit are always used not selectable Agilent 1200 Infinity Series DAD User Manual 213 12 Hardware Information 214 The RS 232C is designed as DCE data communication equipment with a 9 pin male SUB D type connector The pins are defined as Table 16 RS 232C Connection Table Pin Direction Function 1 In DCD 2 In RxD 3 Out TxD 4 Out DTR 5 Ground 6 In DSR 7 Out RTS 8 In CTS 9 In RI Instrument PC DCD 1 1 DCD RX 2 a 2 RX TX 3 PAN 3 1X DTR 4 ay 4 DTR GND 5 X 5 GND DSR 6 6 DSR RTS 7 V 7 RTS CTS 8 A 8 CTS RI 9 9 RI DB9 DB9 DB9 DB9 Male Female Female Male Figure 53 RS 232 Cable Analog Signal Output The analog signal output can be distributed to a recording device For details refer to the description of the module s main board Agilent 1200 Infinity Series DAD User Manual Hardware Information 12 APG Remote The APG Remote connector may be used in combination with other analytical instruments from Agilent Technologies if you want to use features as common shut down prepare and so on Remote control allows easy connection between single instruments or systems to ensure coordinated analysis with simple coupling requirements The subminiature D connector is used The module provides one remote connector which is inputs outputs wired or technique To provide maximum safety within a distributed analysis system one line is dedicated to SHUT DOWN t
21. sensitivity to that molecule The detector is linear up to 2 AU and beyond for many applications This offers a wide linear range for concentration For high concentration analysis the concentration linear range can be extended by setting the wavelength to one with a lower absorbance such as a wavelength minimum or by taking a wider bandwidth which usually includes lower absorbance values The use of wavelength maxima and minima for quantitation dates back to conventional UV detectors which because of mechanical tolerances in moving gratings needed to avoid steeply sloping parts of the spectrum Diode array based detectors do not have this limitation but for reasons of convention maxima and minima are chosen in preference to other parts of the spectrum The reference bandwidth is normally set on a region of the UV spectrum in which the analyte has no absorbance This is shown in the spectrum for anisic acid in Figure 29 on page 104 This spectrum is typical of many small molecules containing a UV chromophore For best results the reference has been set so that it is a wide band as close to the signal wavelength as possible but on a zero absorbance region Reference bandwidths of 60 nm to 100 nm are commonly used The default reference is 360 nm with a bandwidth of 100 nm A wide bandwidth is used because this reduces the noise in the reference signal from statistical theory the error i e noise in this case is reduced by the square root of the num
22. 0 062 80 80 80 80 40 gt 0 006 0 12 40 40 40 40 40 gt 0 012 0 25 20 20 20 20 20 gt 0 025 0 5 10 10 10 10 10 gt 0 05 1 0 5 5 5 5 5 gt 0 10 2 0 2 5 2 5 2 5 2 5 2 5 gt 0 20 4 0 1 25 1 25 1 25 1 25 1 25 gt 0 40 8 0 0 625 0 62 0 625 0 625 0 625 gt 0 85 16 0 0 3125 0 31 0 3125 0 3125 0 3125 1 2 64212A only 88 Values in the User Interface may be rounded The maximum spectra scan rate depends on the data points per scan see Table 8 on page 88 Running at 160 Hz the spectra scan data rate is reduced automatically if the spectra scan data rate is more than 251 points scan Agilent 1200 Infinity Series DAD User Manual Optimizing the Detector 5 Sample and Reference Wavelength and Bandwidth The detector measures absorbance simultaneously at wavelengths from 190 to 640 nm A UV lamp provides good sensitivity over the whole wavelength range If you know little about the analytes in your sample store all spectra over the full wavelength range This provides full information but fills up your disk space rather quickly Spectra can be used to check a peak s purity and identity Spectral information is also useful to optimize wavelength settings for your chromatographic signal The detector can compute and store at run time up to 8 signals with these properties e sample wavelength the center of a wavelength band with the width of sample bandwidth BW and optionally e reference wavelength the center of a wavelen
23. 184 Agilent 1200 Infinity Series DAD User Manual Maintenance 9 Information from Module s Assemblies Lamp and Flow Cell RFID Tag The detector is equipped with a UV lamp and flow cell identification system using RFID radio frequency identification tags attached to the assemblies and RFID tag readers at the optical unit The table below lists all parameters stored in the RFID tag Table 14 RFID Tag Data Lamp information Flow cell information product number product number serial number e serial number production date production date accumulated UV on time in hours nominal path length of the cell in mm actual UV lamp on time in hours cell volume 6 in uL number of ignitions maximum pressure in bar date of last intensity test date of last cell test The pressure value is always displayed in bar even if the user interface uses other units e g PSI Serial Number and Firmware Revision The user interface provides module specific information that is stored in the main board These are for example the serial number firmware revision Agilent 1200 Infinity Series DAD User Manual 185 9 Maintenance Information from Module s Assemblies 186 Agilent 1200 Infinity Series DAD User Manual Agilent 1200 Infinity Series DAD User Manual a e e ee 10 7 e Parts and Materials for Maintenance amp e Overview of Maintenance Parts 188 Kits 190 Accessory Kit 190 Inline
24. 2 s PW gt 0 10 min 2 0 s response time 2 5 Hz PW lt 0 0016 min 0 016 s response time 160 Hz Response time 0 016 s Slit width 4 nm o os 1 15 2 25 3 35 4 45 mia Figure 19 Influence of Response Time on Signal and Noise Table 8 on page 88 lists the filter choices of the detector To get optimum results set peak width as close as possible to a narrow peak of interest in your chromatogram Response time will the be approximately 1 3 of the peak width resulting in less than 5 peak height reduction and less than 5 additional peak dispersion Decreasing the peak width setting in the detector will result in less than 5 gain in peak height but baseline noise will increase by a factor of 1 4 for a factor of 2 response time reduction Increasing peak width response time by factor of two from the recommended setting over filtering will reduce peak height by about 20 and reduce baseline noise by a factor of 1 4 This gives you the best possible signal to noise ratio but may affect peak resolution Agilent 1200 Infinity Series DAD User Manual 87 5 Optimizing the Detector Table 8 Peak Width Response Time Data Rate Peak width at half Response Signaldata Scan data rate Scan data rate Scan data rate Scan data rate height min s rate Hz HZ HZ HZ HZ 126 pts scan 251 pts scan 501 pts scan gt 501 pts scan lt 0 0016 0 016 1602 1602 80 40 20 gt 0 0016 0 03 1602 1602 80 40 20 gt 0 003
25. 3 3 2 4 6 5 5 6 4 7 8 8 7 9 9 5181 1561 RS 232 cable 8 m Agilent 1200 Infinity Series DAD User Manual 193 11 Identifying Cables Analog Cables One end of these cables provides a BNC connector to be connected to Agilent modules The other end depends on the instrument to which connection is being made Agilent Module to 3394 6 Integrators p n 35900 60750 Pin 3394 6 Pin Agilent Signal Name module 1 Not connected 2 Shield Analog 7 oe 3 Center Analog Agilent Module to BNC Connector p n 8120 1840 Pin BNC Pin Agilent Signal Name module O Shield Shield Analog E l Ne Center Center Analog 194 Agilent 1200 Infinity Series DAD User Manual Agilent Module to General Purpose Identifying Cables 11 p n 01046 60105 Pin Pin Agilent Signal Name module 1 Not connected 2 Black Analog 3 Red Analog S e Agilent 1200 Infinity Series DAD User Manual 195 11 Identifying Cables Remote Cables 196 One end of these cables provides a Agilent Technologies APG Analytical Products Group remote connector to be connected to Agilent modules The other end depends on the instrument to be connected to Agilent Module to 3396A Integrators p n 03394 60600 Pin 3396A Pin Agilent Signal Name Active module TTL 9 1 White Digital ground NC 2 Brown Prepare run Low 80 A 3 3 Gray Start Low z NC 4
26. 661 215 re Figure 37 Cell Test Results Agilent 1200 Infinity Series DAD User Manual 143 8 Test Functions and Calibration Cell Test 2 aa 1 1467E 05 Intensity Spectrum with Flow Ce Figure 38 Cell Test Signals Test Failed low ratio value Cell Test Evaluation Probable cause Suggested actions 1 Absorbing solvent or air bubble in flow cell Ensure the flow cell is filled with water and free from air bubbles 2 Dirty or contaminated flow cell Clean the flow cell as described in Cleaning of Max Light Cartridge Cell on page 178 144 Agilent 1200 Infinity Series DAD User Manual Test Functions and Calibration 8 Quick Noise Test The quick noise test measures the noise of the detector with Max Light Cartridge Cell or with Max Light Cartridge Test Cell installed in one minute intervals over a total of 5 minutes The noise of the detector is calculated by using the maximum amplitude for all random variations of the detector signal of frequencies greater than one cycle per hour The noise is determined for 5 one minute intervals and is based on the accumulated peak to peak noise for the intervals At least seven data points per cycles are used in the calculation The cycles in the noise determination are not overlapping If the test is performed with the Max Light Cartridge Test Cell the test results are not influenced by solvent or pump effects When In case of noise and drift problem Parts r
27. 7 nm Autobalance Lamps on required for aquisition V Prerun UV Lamp Postrun Figure 17 Method Parameter Settings The 1260 Infinity DAD G4212B has a fixed slit width of 4 nm 60 Agilent 1200 Infinity Series DAD User Manual Spectrum Settings Store Spectrum Store None he Apex nm Apex Baselines Apex Slopes hy Apex Slope Baselines Allin Peak NERE Every 2nd Spectrum All Nutan 1 None Apex Apex Baselines Apex Slopes Apex Slopes Baselines All in Peak Every 2nd spectrum Range Agilent 1200 Infinity Series DAD User Manual Using the Module 4 Defines at which points on signal A spectra will be taken and saved Signal A is used to control the peak controlled spectra acquisition the other signals have no influence on spectra acquisition Limits 190 0 to 640 0 nm in steps of 0 1 nm for both low and high values The high value must be greater than the low value by at least 0 1 nm No spectra are taken Spectra are taken at the apex of the peak Spectra are taken at the apex and baselines of the peak Spectra are taken at the apex upslope and down slope of the peak Spectra are taken at the apex baselines upslope and down slope of the peak All spectra within the peak are taken Spectra are taken continuously as for All but only every second spectrum is stored other spectra are discarded This reduces the amount of data storage necessary Range defines the wavelengt
28. 7763 Counts Figure 49 Dark Current Test Results Agilent 1200 Infinity Series DAD User Manual 161 8 Test Functions and Calibration Dark Current Test Figure 50 Dark Current Test Signals Test Failed Probable cause Suggested actions 1 Defective slit assembly stray light Run the Slit Test G4212A on page 151 part of the Self Test on page 138 2 Defective detector main board Please contact your Agilent service representative 3 Defective PDA optical unit Please contact your Agilent service representative 162 Agilent 1200 Infinity Series DAD User Manual Agilent 1200 Infinity Series DAD User Manual 9 Maintenance Warnings and Cautions 164 Introduction to Maintenance 166 Overview of Maintenance 167 Cleaning the Module 168 Replacing the Deuterium Lamp 169 Replacing the Max Light Cartridge Cell 173 Cleaning of Max Light Cartridge Cell 178 Storage of Max Light Cartridge Cell 179 Drying the Leak Sensor 180 Replacing Leak Handling System Parts 181 Replacing Module Firmware 183 Information from Module s Assemblies 185 This chapter describes the maintenance of the module ee Agilent Technologies 163 9 Warnings and Cautions WARNING Toxic flammable and hazardous solvents samples and reagents The handling of solvents samples and reagents can hold health and safety risks gt When working with these substances observe appropriate safety procedures for example by wearing
29. 92 Optimizing Spectral Acquisition 94 Margin for Negative Absorbance 95 Optimizing Selectivity 96 Quantifying Coeluting Peaks by Peak Suppression 96 Ratio Qualifiers for Selective Detection of Compound Classes 98 Optimizing the Detector Regarding to the System 100 Delay Volume and Extra Column Volume 100 How to Configure the Optimum Delay Volume 100 How to Achieve Higher Sensitivity 101 Warm up of the Detector 107 This chapter provides information on how to optimize the detector oh Agilent Technologies 77 5 Optimizing the Detector Introduction The detector has a variety of parameters that can be used to optimize performance Depending on whether signal or spectral data need to be optimized different settings are recommended The following sections describe optimization for e signal sensitivity selectivity and linearity e spectral sensitivity and resolution DAD only and e disk space required for storing data The information in this chapter should be seen as a basic introduction to diode array detector techniques Some of these techniques may not be available in the instrument software controlling the detector How to Get the Best Detector Performance The information below will guide you on how to get the best detector performance Follow these rules as a start for new applications It gives rules of thumb for optimizing detector parameters 78 Agilent 1200 Infinity Series DAD User Manual Optimizing the Detector 5
30. Agilent lamp used You may be using the device outside of the guaranteed specification C_m 56 Lamps can be turned ON OFF At Power On automatic lamp on at power on Analog Output Range can be set to either 100 mV or 1 V full scale 1 V default UV lamp Tag Automatic detects a lamp with RFID tag If no RFID tag lamp is used UV lamp not ready is displayed and it cannot be ignited A compatible mode has to be selected based on the used lamp see Non RFID tag lamp information below Automatic Turn On The module can be turned on ata specified date time If Turn UV lamp on at power on is set the lamp is turned on as well Non RFID tag lamp In case a non RFID tag lamp is used the user interface will show this when selecting a compatible mode You may operate the detector outside of the guaranteed specification Agilent 1200 Infinity Series DAD User Manual Using the Module 4 Setting up the Detector with Agilent ChemStation Method Parameter Settings NOTE These settings are available via Menu Instrument Setup Instrument Method or via right click on the active area of the detector GUI The Instrument Curves tab is not shown when opening the method parameter settings via right mouse click on the detector GUI Switches to additional instrument signals for troubleshooting Toggles to Advanced Settings actual window Toggles to Timetable Settings Opens the Time table graph
31. Application Data Agilent BootP LogFile It contains a MAC address entry for each device that requests configuration information from BootP 3 Click OK to save the values or Cancel to discard them The editing ends 4 After each modification of the BootP settings i e EditBootPSettings a stop or start of the BootP service is required for the BootP service to accept changes See Stopping the Agilent BootP Service on page 243 or Restarting the Agilent BootP Service on page 244 5 Uncheck the Do you want to log BootP requests box after configuring instruments otherwise the log file will quickly fill up disk space Agilent 1200 Infinity Series DAD User Manual 239 13 LAN Configuration 240 Determining the MAC address directly from the LAN Interface card label 1 Turn off the instrument 2 Read the MAC address from the label and record it The MAC address is printed on a label on the rear of the module See Figure 55 on page 224 and Figure 56 on page 224 3 Turn on the instrument Assigning IP Addresses Using the Agilent BootP Service The Agilent BootP Service assigns the Hardware MAC address of the instrument to an IP address Determining the MAC address of the instrument using BootP Service 1 Power cycle the Instrument 2 After the instrument completes self test open the log file of the BootP Service using Notepad The default location for the logfile is C Documents and Settings All Users Application D
32. Cell only Both bio inert Max Light Cartridge Flow Cells include PEEK tubing 1 16 0890 1763 and e Fingertight fitting long 5062 8541 Recommendations Assure the following The capillary ends are right angled when cutting capillaries No pliers or wrenches are used to fix the PEEK fittings at the flow cell No metal ferrules are used at the cell unions to prevent contaminations and damage e the flow cell is bypassed when flush procedures at pH gt 12 5 are used Application Information For the analysis and characterization of proteins and large biomolecules for SEC AEX and RP applications add 100 mM salt into mobile phase or 10 organic to prevent secondary interaction For cation exchange chromatography the usage of an Agilent 1260 Infinity Diode Array Detector G1315C D with the respective bio inert flow cell is highly recommended to avoid unspecific interaction of the protein with the flow cell For applications with mobile phases of a pH above 12 5 use an Agilent 1260 Infinity Diode Array Detector G1315C D and the respective bio inert flow cell Agilent 1200 Infinity Series DAD User Manual Optimizing the Detector 5 Optimizing for Sensitivity Selectivity Linearity and Dispersion Flow Cell Path Length Lambert Beer s law shows a linear relationship between the flow cell path length and absorbance Absorbance log T log a exCxd where T is the transmission defined as the quotient of the intens
33. For example when connected to a non negotiating 10 Mbps hub the LAN interface will be automatically set to operate at 10 Mbps half duplex If the module is not able to connect to the network through auto negotiation you can manually set the link operating mode using link configuration switches on the module Table 26 Link Configuration Switches SW3 SW4 SW5 Link Configuration OFF speed and duplex mode determined by auto negotiation ON OFF OFF manually set to 10 Mbps half duplex ON OFF ON manually set to 10 Mbps full duplex ON ON OFF manually set to 100 Mbps half duplex ON ON ON manually set to 100 Mbps full duplex 234 Agilent 1200 Infinity Series DAD User Manual LAN Configuration 13 Automatic Configuration with BootP All examples shown in this chapter will not work in your environment You need your own IP Subnet Mask and Gateway addresses Assure that the detector configuration switch is set properly The setting should be either BootP or BootP amp Store see Table 22 on page 227 Assure that the detector connected to the network is powered off If the Agilent BootP Service program is not already installed on your PC then install it from your Agilent ChemStation DVD located in folder BootP About Agilent BootP Service The Agilent BootP Service is used to assign the LAN Interface with an IP address The Agilent BootP Service is provided on the ChemStation DVD The Agilent BootP Service is in
34. Maintenance required EMF warning Maintenance or check might be required yellow EMF warning Maintenance required red Important The EMF settings can be accessed via the Agilent Lab Advisor or the Instant Pilot only The limit s can be changed Based on the limit the User Interface displays the above status RFID tag information is displayed when moving with the mouse cursor on to the tag attached to the flow cell or lamp The information provides flow cell and lamp related information like e Part number e Production date Serial number and other details Lamp tag information Burn time 93 3h Minimum lifetime 2000 0h Number of ignitions 10 Product Number 5190 0917 Serial Number 824337 Production Date 4 9 2009 8 23 53 AM Tested Date 7 16 2009 1 50 04 PM Intensity at test 37275 counts Cell tag information Cell Name Max Light Cell Product Number 64212 60008 Serial Number 10PP042325 Production Date 2 5 2009 12 49 06 PM Optica path length 10 0mm Cell Volume 0 1 0 pL Maximum pressure 60 bar Tasted Date 7 10 2009 1 44 52 PM Call Revision 0 55 4 Using the Module Control Settings ioixi Lamps At Power On uv On Tum UV lamp on Off Analog Output Range Output 1 01y 1v UV Lamp Tag Automatic use lamp tag 5190 0917 compatible without continuous heating 5190 0916 compatible continuous heating Automatic Tum On Turn on at Ok Cancel Help warmina x A Non original
35. No Yes Yes 1 Yes K4212B DAD Clinical Ed G1315C DAD VL 2 No Yes Yes 2 Yes G1365C MWD G1315D DAD VL G1365D MWD VL G1321B FLD 2 Yes No Yes 2 Yes K1321B FLD Clinical Ed G1362A RID 2 Yes No Yes 1 Yes G4280A ELSD No No No Yes Yes Yes EXT Contact AUTOZERO Others G1170A Valve Drive 2 No No No No No 1 G1316A C TCC 2 No No Yes No Yes K1316C TCC Clinical Ed G1322A DEG No No No No No Yes AUX K1322A DEG Clinical Ed G1379B DEG No No No Yes No Yes G4225A DEG No No No Yes No Yes K4225A DEG Clinical Ed Agilent 1200 Infinity Series DAD User Manual 211 12 Hardware Information Table 15 Agilent 1200 Infinity Series Interfaces Module CAN LAN BCD LAN RS 232 Analog APG Special optional on board Remote G4227A Flex Cube 2 No No No No No CAN DC OUT for CAN slaves 1 G4240A CHIP CUBE 2 Yes No Yes No Yes CAN DC OUT for CAN slaves THERMOSTAT for G1330A B NOT USED K1330B 1 Requires a HOST module with on board LAN e g G4212A or G4220A with minimum firmware B 06 40 or C 06 40 or with additional G1369C LAN Card The detector DAD MWD FLD VWD RID is the preferred access point for control via LAN The inter module communication is done via CAN e CAN connectors as interface to other modules e LAN connector as interface to the control software e RS 232C as interface to a computer e REMOTE connector as interface to other Agilent products Analog output connector s for signal output 212 Agilent 1
36. System Parts When If the parts are corroded or broken Tools required Description Tissue Parts required p n Description 1 5061 3388 Leak funnel 1 5041 8389 Leak funnel holder 1 5062 2463 Corrugated tubing PP 6 5 mm id 5m 1 G4212 40027 Leak downpipe Preparations Turn the pump off 1 Remove the front cover 2 Locate the leak interface area Agilent 1200 Infinity Series DAD User Manual 181 9 Maintenance Replacing Leak Handling System Parts 3 Pull the leak funnel out of the leak funnel holder and slide the Leak downpipe up for removal ane TALITITITET OREIN 4 Insert the leak interface system parts Ensure the tubing is fitted correctly in the bottom 5 Close the front cover 182 Agilent 1200 Infinity Series DAD User Manual Maintenance 9 Replacing Module Firmware When Tools required OR OR Parts required Preparations The installation of newer firmware might be necessary if a newer version solves problems of older versions or to keep all systems on the same validated revision The installation of older firmware might be necessary to keep all systems on the same validated revision or if anew module with newer firmware is added to a system or if third party control software requires a special version Description LAN RS 232 Firmware Update Tool Agilent Lab Advisor software Instant Pilot G4208A only if supported by mod
37. TYPE G1315 66565 SER MAC REV AC MFG 2140 0820 848728 no info Figure 63 LAN Setting on Instant Pilot 2 Set the Configuration Switch to DHCP either on the G1369C LAN Interface Card or the main board of above mentioned modules Table 24 G1369C LAN Interface Card configuration switch on the card SW 4 SW 5 SW 6 SW7 SW 8 Initialization Mode ON OFF OFF OFF OFF DHCP 232 Agilent 1200 Infinity Series DAD User Manual LAN Configuration 13 Table 25 LC Modules inclusive 1120 1220 configuration switch at rear of the instru ment SW6 SW7 SW 8 Initialization Mode ON OFF OFF DHCP 3 Turn on the module that hosts the LAN interface 4 Configure your Control Software e g Agilent ChemStation LabAdvisor Firmware Update Tool and use MAC address as host name e g 003043177321 The LC system should become visible in the control software see Note in section General Information DHCP on page 231 Agilent 1200 Infinity Series DAD User Manual 233 13 LAN Configuration Link configuration selection The LAN interface supports 10 or 100 Mbps operation in full or half duplex modes In most cases full duplex is supported when the connecting network device such as a network switch or hub supports IEEE 802 3u auto negotiation specifications When connecting to network devices that do not support auto negotiation the LAN interface will configure itself for 10 or 100 Mbps half duplex operation
38. V o 1 0 uL G5615 60018 offer highest sensitivity for bio inert reverse phase applications Please note that at low salt SEC or ion exchange chromatography potentially peak tailing might occur and therefore for these applications the universal Bio inert DAD G1315C or D or MWD G1365C or D is recommended Table 1 Bio inert materials used in Agilent 1260 Infinity Systems Module Materials Agilent 1260 Infinity Bio inert Quaternary Pump Titanium gold platinum iridium G5611A ceramic ruby PTFE PEEK Agilent 1260 Infinity Bio inert High Performance Autosampler Upstream of sample introduction G5667A e Titanium gold PTFE PEEK ceramic Downstream of sample introduction PEEK ceramic Agilent 1260 Infinity Bio inert Manual Injector PEEK ceramic G5628A Agilent 1260 Infinity Bio inert Analytical Fraction Collector PEEK ceramic PTFE G5664A Agilent 1200 Infinity Series DAD User Manual 17 1 18 Introduction Table 1 Bio inert materials used in Agilent 1260 Infinity Systems Module Materials Bio inert Flow Cells Standard flow cell bio inert 10 mm 13 pL 120 bar 12 MPa for MWD DAD includes Capillary Kit Flow Cells BIO p n G5615 68755 G5615 60022 for Agilent 1260 Infinity Diode Array Detectors DAD G1315C D Max Light Cartridge Cell Bio inert 10 mm V o 1 0 pL G5615 60018 and Max Light Cartridge Cell Bio inert 60 mm V o 4 0 pL G5615 60017 for Agilent
39. for maintenance 188 P packaging damaged 30 parts identification accessory kit 190 PC and Agilent ChemStation Setup 251 PC setup local configuration 251 peak width response time 87 peak width 105 performance optimization 78 specifications 24 26 photometric accuracy 86 physical specifications 23 273 Index power considerations 20 power consumption 23 power cords 21 power supply indicator 111 programmable slit width 24 26 Q quick noise test 145 radio interference 267 recalibration initial 48 recyclable material 25 27 remote cable 196 repairs cautions and warnings 164 replacing firmware 183 response time peak width 87 response time versus time constant 28 response time 105 RS 232C cable 202 S safety class 263 safety general information 263 standards 23 symbols 262 sample and reference wavelength 89 selectivity optimization 96 self test 138 serial number information 209 209 setup ChemStation 254 274 detector 51 shutdown 120 signal wavelength 103 site requirements power cords 21 slittest 151 slitwidth 24 26 92 slit fix 15 programmable 14 solvent information 73 solvents 269 sound emission 268 special interfaces 216 special settings boot resident 219 forced cold start 219 specification physical 23 specifications analog signal output 25 27 communications 25 27 diode width 24 26 flow cell 25 27 GLP features 25 27 linear range 24 26 noise and drift A
40. give between three and five times increase in signal to noise values depending on the application conditions The dispersion volume is fractionally increased compared to the standard cell Agilent 1200 Infinity Series DAD User Manual 101 5 102 Optimizing the Detector Wavelength and Bandwidth The detector measures absorbance simultaneously at wavelengths from 190 nm to 640 nm using diode array detection A UV lamp provides good sensitivity over the whole wavelength range The diode array detector DAD can simultaneously compute and send to the data system up to eight chromatographic signals and the full range spectra at every time point A UV chromatogram or signal is a plot of absorbance data versus time and is defined by its wavelength and bandwidth e The wavelength indicates the center of the detection band The bandwidth defines the wavelength range over which the absorbance values are averaged to give the result at each time point For example a signal at wavelength 250 nm with a bandwidth of 16 nm will be an average of the absorbance data from 242 nm to 258 nm Additionally a reference wavelength and reference bandwidth can be defined for each signal The average absorbance from the reference bandwidth centered on the reference wavelength will be subtracted from its equivalent value at the signal wavelength to produce the output chromatogram The signal wavelength and bandwidth can be chosen so that they are optimized
41. goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the vendor and follow good laboratory practice gt The volume of substances should be reduced to the minimum required for the analysis gt Do not operate the instrument in an explosive atmosphere Eye damage by detector light 0 Eye damage may result from directly viewing the UV light produced by the lamp of the optical system used in this product gt Always turn the lamp of the optical system off before removing it Electrical shock Repair work at the module can lead to personal injuries e g shock hazard when the cover is opened gt Do not remove the cover of the module gt Only certified persons are authorized to carry out repairs inside the module 164 Agilent 1200 Infinity Series DAD User Manual Maintenance 9 Personal injury or damage to the product Agilent is not responsible for any damages caused in whole or in part by improper use of the products unauthorized alterations adjustments or modifications to the products failure to comply with procedures in Agilent product user guides or use of the products in violation of applicable laws rules or regulations gt Use your Agilent products only in the manner described in the Agilent product user guides CAUTION Safety standards for external equipment gt If you connect external equipment to the instrument
42. in subsequent sections Solvent information for parts of the 1260 Infinity Bio inert LC system For the Agilent 1260 Infinity Bio inert LC system Agilent Technologies uses highest quality materials see Bio inert Materials on page 17 in the flow path also referred to as wetted parts which are widely accepted by life scientists as they are known for optimum inertness to biological samples and ensure best compatibility to common samples and solvents over a wide pH range Explicitly the complete flow path is free from stainless steel and free from other alloys containing metals such as iron nickel cobalt chromium molybdenum or copper which can interfere with biological samples The flow downstream of the sample introduction contains no metals whatsoever However there are no materials that combine suitability for versatile HPLC instrumentation valves capillaries springs pump heads flow cells etc with complete compatibility with all possible chemicals and application conditions This section recommends the preferred solvents Chemicals that are known to cause issues should be avoided or exposure should be minimized for example for short term cleaning procedures After potentially aggressive chemicals have been used the system should be flushed with compatible standard HPLC solvents Agilent 1200 Infinity Series DAD User Manual 73 4 74 Using the Module PEEK PEEK Polyether Ether Ketones combines excellent p
43. instrument with a remote connection to the system 3 Shut down in an external instrument with a remote connection to the system Remote Timeout Error ID 0070 Suggested actions Fix the leak in the external instrument before restarting the module Fix the leak in the external instrument before restarting the module Check external instruments for a shut down condition A not ready condition is still present on the remote input When an analysis is started the system expects all not ready conditions for example a not ready condition during detector balance to switch to run conditions within one minute of starting the analysis If a not ready condition is still present on the remote line after one minute the error message is generated Probable cause 1 Not ready condition in one of the instruments connected to the remote line 2 Defective remote cable 3 Defective components in the instrument showing the not ready condition Suggested actions Ensure the instrument showing the not ready condition is installed correctly and is set up correctly for analysis Exchange the remote cable Check the instrument for defects refer to the instrument s documentation Agilent 1200 Infinity Series DAD User Manual Error Information 7 Lost CAN Partner Error ID 0071 During an analysis the internal synchronization or communication between one or more of the modules in the system has failed The system processo
44. make sure that you only use accessory units tested and approved according to the safety standards appropriate for the type of external equipment Agilent 1200 Infinity Series DAD User Manual 165 9 Maintenance Introduction to Maintenance The module is designed for easy maintenance Maintenance can be done from the front with module in place in the system stack There are no serviceable parts inside Do not open the module 166 Agilent 1200 Infinity Series DAD User Manual Overview of Maintenance Maintenance 9 The following pages describe maintenance simple repairs of the detector that can be carried out without opening the main cover Table 12 Overview of Maintenance Procedure Typical Frequency Notes Cleaning of module If required Deuterium lamp If noise and or drift exceeds your application limits or A wavelength calibration test and an exchange lamp does not ignite intensity test should be performed after replacement Flow cell exchange If leaking or if intensity drops due to contaminated flow Awavelength calibration test should be cell performed after replacement Leak sensor drying If leak has occurred Check for leaks Leak handling System If broken or corroded replacement Check for leaks Agilent 1200 Infinity Series DAD User Manual 167 9 Maintenance Cleaning the Module To keep the module case clean use a soft cloth slightly dampened with water or a solution of water and mild d
45. particular class needs to be analyzed a parent drug and its metabolites in a biological sample for example Another example is the selective analysis of derivatives after pre or post column derivatization Specifying a signal ratio that is typical for the sample class is one way of selectively plotting only those peaks that are of interest The signal output remains at zero so long as the ratio is out of the user specified ratio range When the ratio falls within the range the signal output corresponds to the normal absorbance giving single clear peaks on a flat baseline An example is shown in Figure 27 on page 98 and Figure 28 on page 99 Select 2 characteristic 1287 wavelengths j Signal WL1 WL2 20 oTerphenyl 6e4 5a Biphenyl j 4a4 204 Scaled Wavelength nm Figure 27 Wavelength Selection for Ratio Qualifiers 98 Agilent 1200 Infinity Series DAD User Manual Optimizing the Detector 5 Signals at 250 nm Biphenyl o Terphenyl No selectivity With ratio qualifier 249 224 nm 3 520 Time jimin Figure 28 Selectivity by Ratio Qualifiers In a four component mixture only biphenyl was recorded The other three peaks were suppressed because they did not meet the ratio qualifier criterion and therefore the output was set to zero The characteristic wavelengths 249 nm A and 224 nm A were found from the spectra shown in Figure 27 on page 98 The ratio range was set at 2 2 4 2 2 10 Only w
46. reference Time min Grad 0 02 m KH P0 ACN from 12 ACN to 45 ACN in 12 min Figure 22 Gradient Analysis of PTH Amino Acids 1 pmol each with and without Reference Agilent 1200 Infinity Series DAD User Manual 91 5 Optimizing the Detector Slit Width G4212A The 1290 Infinity DAD G4212A has a variable slit at the entrance of the spectrograph This is an effective tool to adapt the detector to changing demand of different analytical problems A narrow slit provides spectral resolution for analytes with very fine structures in the absorbance spectrum An example of such a spectrum is benzene The five main absorbance bands fingers are only 2 5 nm wide and just 6 nm apart from each other 240 245 250 255 Figure 23 Benzene at 1 and 4 nm slit width principle A wide slit uses more of the light shining through the flow cell This gives lower baseline noise as shown in Figure 24 on page 93 92 Agilent 1200 Infinity Series DAD User Manual Optimizing the Detector 5 DADI A Sige240 100 Rete200 100 G42 1ZAINOL_SwW_1_D mau Slit width 8 nm DADE A Sig 260 100 Ret360 100 G42 12A NOL_SW_8 0 mau Slit width 1 nm Pe er a eah aaisa iiaii Data Rate 160 Hz o os 1 15 2 25 2 2s 4 as min Figure 24 Influence of the Slit Width on Baseline Noise However with a wider slit the spectrograph s optical resolution its ability to distinguish between different wavelengths diminishes Any photodi
47. scan the alpha and beta line deviations in nm are displayed These values indicate how far the detector calibration deviates from the actual positions of the alpha and beta emission lines After calibration the deviation is zero To eliminate effects due to absorbing solvents install the Max Light Cartridge Test Cell before starting the test Improved wavelength calibration algorithm The wavelength calibration algorithm has been changed to higher accuracy in UV wavelength range for G4212A B DAD and K4212B DAD Clincal ed with firmware B 06 33 It has been found that after a wavelength re calibration using the Agilent LabAdvisor or Instant Pilot the measured wavelength in the lower UV might be out of the specified range of 1 1 nm Example Using Caffeine and measuring at 205 nm The higher wavelength range is not effected The detector is calibrated at the factory and under normal operating conditions should not require recalibration However it is advisable to recalibrate after maintenance flow cell or UV lamp after repair of components in the optical unit after exchange of the optical unit or main board e after significant environmental condition changes temperature humidity ata regular interval at least once per year for example prior to an Operational Qualification Performance Verification procedure and when chromatographic results indicate the detector may require recalibration Descr
48. screen shows all modules of the system The Control screen allows e Lamp On Off Get Ready e Reset of Errors Balance Take Spectrum 69 4 Using the Module Main Screens of the Detector with Agilent Instant Pilot G4208A TYPE 64208 66500 SER 001077 REV B 02 00 y DAD PROO100015 B 06 23 0003 B 06 20 0001 Od 03 34h USING STORED 192 168 254 11 0030D317521C TYPE 64212 65800 REV BE MFG ZZ SER M 5190 0917 824337 4212 60011 200PP00015 Use lamp tag settings lov 1V_ output range LAN Subnet Mask LAN Def Gateway Enter up to 30 characters eT 16 43 70 Stays off at power on 192 168 254 11 Mask 255 255 255 000 255 255 255 255 Cancel Done The System Info screen list details of the detector Firmware revision On time LAN settings e Main Board information Lamp RFID tag Information Flow cell RFID tag Information The Configuration screen allows to configure Symbolic name of module Temperature control Lamp and cell RFID tag use Analog Output range UV lamp at power on LAN settings Agilent 1200 Infinity Series DAD User Manual Using the Module 4 Main Screens of the Detector with Agilent Instant Pilot G4208A The Method screen lists all method parameters of the detector These can be edited wv Method DEFAULT all j ef 0 0nm Signal C 214 0 4 0 Ref 360 0 100 0 nm Signal D 230 0 4 0
49. should be less than 3 uV Probable cause 1 Bad cable or grounding problem between detector and external device 2 Defective detector main board 160 Suggested actions Check or replace the cable Please contact your Agilent service representative Agilent 1200 Infinity Series DAD User Manual Test Functions and Calibration 8 Dark Current Test The dark current test measures the leakage current from each diode The test is used to check for leaking diodes which may cause non linearity at specific wavelengths During the test the slit assembly moves to the dark position cutting off all light falling onto the diode array Next the leakage current from each diode is measured and displayed graphically The leakage current represented in counts for each diode should fall within the limits When In case of problem 1 Run the Dark Current Test with the recommended user interface for further information see Online Help of user interface Test Name Dark Current Test Description The test measures the dark current from the detector optic Module G4212A PR00100015 Status Passed Start Time 7 9 2009 3 04 21 PM Stop Time 7 9 2009 3 04 41 PM ENERENENENEEENNEN p Test Procedure Resut i Name Value 1 Check Prerequisites Cet Product Number G4212 60011 i Y 2 PotomDak Curent Test Y 2 Evawe Daa Lamp Type Automatic Mode Dark Current Minimum 7698 Counts Dark Curent Average Dark Current Maximum
50. tests are available Agilent Lab Advisor All tests are available Agilent ChemStation No tests available Adding of temperature lamp signals to chromatographic signals possible Agilent Instant Pilot Some tests are available Intensity Cell WL Calibration Self Test Intensity Quick Noise ASTM Drift and Noise Cell Dark Current D A Converter Slit G4212A only WL Verification WL Calibration Test Chromatogram Tools Spectra Scan Tools Module Infos Tools Diagnostic Tools Temperature main board Temperature optical unit Lamp anode voltage Intensity WL Calibration Cell For details on the use of the interface refer to the interface documentation 134 Agilent 1200 Infinity Series DAD User Manual Test Functions and Calibration 8 Use of Max Light Cartridge Test Cell The Max Light Cartridge Test Cell is recommended to be used for several tests instead of the Max Light Cartridge Cell 10 mm V 1 uL or the Max Light Cartridge Cell 60 mm V 4 uL because it allows running the test s without any influence of the rest of the system degasser pump sampler and others The results of the test cell are comparable with the Max Light Cartridge Cell 10 mm V 1 uL filled with water e g Intensity Profile Only the Absorbance value is higher on the Max Light Cartridge Cell If the profile of the Max Light Cartridge Cell differs in the low UV range then absorbing solvents are in the cell
51. 1 2 9 9 Digital ground NC 15 5V Low 200 Agilent 1200 Infinity Series DAD User Manual CAN LAN Cables Identifying Cables 11 Both ends of this cable provide a modular plug to be connected to Agilent modules CAN or LAN connectors CAN Cables p n 5181 1516 5181 1519 LAN Cables p n 5023 0203 5023 0202 Agilent 1200 Infinity Series DAD User Manual Description CAN cable Agilent module to module 0 5 m CAN cable Agilent module to module 1 m Description Cross over network cable shielded 3 m for point to point connection Twisted pair network cable shielded 7 m for point to point connection 201 11 Identifying Cables RS 232 Cables 202 p n G1530 60600 RS232 61601 5181 1561 Description RS 232 cable 2 m RS 232 cable 2 5 m Instrument to PC 9 to 9 pin female This cable has special pin out and is not compatible with connecting printers and plotters It s also called Null Modem Cable with full handshaking where the wiring is made between pins 1 1 2 3 3 2 4 6 5 5 6 4 7 8 8 7 9 9 RS 232 cable 8 m Agilent 1200 Infinity Series DAD User Manual Agilent 1200 Infinity Series DAD User Manual 12 Hardware Information Firmware Description 204 Electrical Connections 207 Rear view of the module 208 Information on Instrument Serial Number 209 Interfaces 210 Overview Interfaces 213 Setting the 8 bit Configuration Switch 217 Special Settings 219 Instrument Layout 220 Ea
52. 1200 Infinity Series Diode Array Detectors DAD G4212A B Bio inert flow cell 8 uL 20 bar pH 1 12 includes Capillary Kit Flow Cells BIO p n G5615 68755 G5615 60005 for Agilent 1260 Infinity Fluorescence Detector FLD G1321B Bio inert heat exchanger G5616 60050 for Agilent 1290 Infinity Thermostatted Column Compartment G1316C Bio inert Valve heads Bio inert Connection capillaries PEEK ceramic sapphire PTFE PEEK fused silica PEEK fused silica PTFE PEEK steel cladded G4235A G5631A G5639A PEEK ceramic Al 03 based Upstream of sample introduction Titanium Downstream of sample introduction Agilent uses stainless steel cladded PEEK capillaries which keep the flow path free of steel and provide pressure stability to more than 600 bar To ensure optimum bio compatibility of your Agilent 1260 Infinity Bio inert LC system do not include non inert standard modules or parts to the flow path Do not use any parts that are not labeled as Agilent Bio inert For solvent compatibility of these materials see Solvent information for parts of the 1260 Infinity Bio inert LC system on page 73 Agilent 1200 Infinity Series DAD User Manual Agilent 1200 Infinity Series DAD User Manual e00 ee 2 r Site Requirements and Specifications Site Requirements 20 Physical Specifications 23 Performance Specifications 24 Specifications 24 Specification Conditions 28 This chapte
53. 200 Infinity Series DAD User Manual Hardware Information 12 Overview Interfaces CAN The CAN is inter module communication interface It is a 2 wire serial bus system supporting high speed data communication and real time requirement LAN The modules have either an interface slot for an LAN card e g Agilent G1369B C LAN Interface or they have an on board LAN interface e g detectors G1315C D DAD and G1365C D MWD This interface allows the control of the module system via a PC with the appropriate control software Some modules have neither on board LAN nor an interface slot for a LAN card e g G1170A Valve Drive or G4227A Flex Cube These are hosted modules and require a Host module with firmware B 06 40 or later or with additional G1369C LAN Card If an Agilent detector DAD MWD FLD VWD RID is in the system the LAN should be connected to the DAD MWD FLD VWD RID due to higher data load If no Agilent detector is part of the system the LAN interface should be installed in the pump or autosampler RS 232C Serial The RS 232C connector is used to control the module from a computer through RS 232C connection using the appropriate software This connector can be configured with the configuration switch module at the rear of the module Refer to Communication Settings for RS 232C There is no configuration possible on main boards with on board LAN These are pre configured for 19200 baud e 8 data bit with no parity and
54. 2013 To protect the flow cell against overpressure e g in systems with LC MS install Inline Pressure Relief Valve Kit G4212 68001 see Inline Pressure Relief Valve Kit G4212 68001 on page 82 Recommendations For G4212 60007 and G4212 60008 The use of Peek FS capillaries is not recommended In combination with the SST zero dead volume fitting e g at the inlet the capillary could break and the glass particles could block damage the flow cell Agilent 1200 Infinity Series DAD User Manual 81 5 Optimizing the Detector Inline Pressure Relief Valve Kit G4212 68001 When several detectors are installed in a system the connecting capillary and fittings between the detectors must be carefully chosen to keep chromatographic influence on peak shape small On the other hand narrow bore connection capillaries generate a significant pressure drop dependent on flow rate and solvent properties The pressure relief valve is designed to protect the flow cell of a Agilent 1200 Series Infinity Diode Array Detector G4212A DAD and G4212B DAD Agilent strongly recommends installing the pressure relief valve at the outlet of the detector as soon as a second detector is installed like in LC MS applications The pressure relief valve with a low internal volume check valve The dead volume is smaller than 100 nL delay volume inlet to outlet The ball of the check valve is spring loaded and adjusted to open at typically 100 bar On over
55. 3177321 It is the DHCP server s responsibility to forward the hostname address information to the Domain Name Server The card does not offer any services for hostname resolution e g NetBIOS DHCP Active Server Parameter Figure 62 DHCP Principle 1 It may take some time until the DHCP server has updated the DNS server with the hostname information 2 It may be necessary to fully qualify the hostname with the DNS suffix e g 0030d3177321 country company com 3 The DHCP server may reject the hostname proposed by the card and assign a name following local naming conventions Agilent 1200 Infinity Series DAD User Manual 231 13 LAN Configuration Setup DHCP Software required The modules in the stack must have at least firmware from set A 06 34 and the above mentioned modules B 06 40 or above must from the same firmware set 1 Note the MAC address of the LAN interface provided with G1369C LAN Interface Card or Main Board This MAC address is on a label on the card or at the rear of the main board e g 0030d3177321 On the Instant Pilot the MAC address can be found under Details in the LAN section System Info Controller DE12345678 G4208A ae Main Revision B 02 12 0001 Reload DAD DE64260019 G1315D Main Revision B 06 41 0002 a Resident Revison B 06 40 0007 Print On time 3d 01 33h Installed Options Dhcp LAN TCP IP Mode DHCP LAN TCP IP Address 130 168 132 219 0030D314F89E
56. 340 360 380 480 Wavelength nm Figure 20 Optimization of Wavelength Setting A wide bandwidth has the advantage of reducing noise by averaging over a wavelength range compared to a 4 nm bandwidth the baseline noise is reduced by a factor of approximately 2 5 whereas the signal is about 75 of a 4 nm wide band The signal to noise ratio for a 30 nm bandwidth is twice that for a 4 nm bandwidth in our example Bandwidth 30 nm 12 nm Figure 21 Influence of Bandwidth on Signal and Noise 90 Agilent 1200 Infinity Series DAD User Manual Optimizing the Detector 5 Because the detector averages absorbance values that are calculated for each wavelength using a wide bandwidth does not negatively impact linearity The use of a reference wavelength is highly recommended to further reduce baseline drift and wander induced by room temperature fluctuations or refractive index changes during a gradient An example of the reduction of baseline drifts is shown in Figure 22 on page 91 for PTH amino acids Without a reference wavelength the chromatogram drifts downwards due to refractive index changes induced by the gradient This is almost completely eliminated by using a reference wavelength With this technique PTH amino acids can be quantified in the low picomole range even in a gradient analysis 1pmol each PTH ASN PTH ARG PTH ALA PTH PRO PTH PHE Wavelength 267 nm Reference 380nm Wavelength 267nm No
57. AD User Manual 41 3 42 Installing the Module 1 Note the MAC address of the LAN interface rear of the module under the configuration switch see Figure 56 on page 224 It s required for LAN Configuration see Chapter LAN Configuration O CONFIG CAN CAN USB 5 _LAN Ol oO s a m J a 0O ab r H e e wm OOO COMPACT FLASH S 2 agentens G CE Product No G4212A pee TTT a MAUA I00C T mane gt gt gt Serial No DEduext10 ott gt gt gt TT E gt gt gt gt hintanen nnman iene A ta 5 Made in Germany AC we aa aw 2 uy oO ol A Figure 15 Rear View of Detector Electrical Connections and Label Set the Configuration Switch according the required initialization mode default fixed IP or Bootp see Chapter LAN Configuration Place the module in the stack see Optimizing the Stack Configuration on page 32 Ensure the line power switch at the front of the module is OFF Connect the power cable to the power connector at the rear of the module Connect the CAN cable to other Agilent modules Connect the LAN cable e g from an Agilent ChemStation as controller to the detector s LAN connector Connect the analog cable optional Connect the APG remote cable optional for non Agilent instruments 10 Turn on power by pushing the button at the lower left hand side of the module The status LED s
58. Agilent Technologies 29 3 Installing the Module Unpacking the Module Damage to the module CAUTION Damaged Packaging If the delivery packaging shows signs of external damage please call your Agilent Technologies sales and service office immediately Inform your service representative that the instrument may have been damaged during shipment Defective on arrival problems If there are signs of damage please do not attempt to install the module Inspection by Agilent is required to evaluate if the instrument is in good condition or damaged gt Notify your Agilent sales and service office about the damage gt An Agilent service representative will inspect the instrument at your site and initiate appropriate actions CAUTION Condensation Condensation within the module Condensation will damage the system electronics gt Do not store ship or use your module under conditions where temperature fluctuations could cause condensation within the module gt If your module was shipped in cold weather leave it in its box and allow it to warm slowly to room temperature to avoid condensation 30 Agilent 1200 Infinity Series DAD User Manual Delivery Checklist Installing the Module 3 Ensure all parts and materials have been delivered with the module The delivery checklist is shown below Please report missing or damaged parts to your local Agilent Technologies sales and service office Table5 Detector C
59. Dynamic Host Configuration Protocol DHCP 231 General Information DHCP 231 Setup DHCP 232 Link configuration selection 234 Automatic Configuration with BootP 235 About Agilent BootP Service 235 How BootP Service Works 236 Situation Cannot Establish LAN Communication 236 Installation of BootP Service 237 Two Methods to Determine the MAC Address 239 Assigning IP Addresses Using the Agilent BootP Service 240 Changing the IP Address of an Instrument Using the Agilent BootP Service 243 Manual Configuration 245 With Telnet 246 With the Instant Pilot G4208A 250 PC and Agilent ChemStation Setup 251 PC Setup for Local Configuration 251 Agilent ChemStation Setup 254 This chapter provides information on connecting the module to the Agilent ChemStation PC ate Agilent Technologies 223 13 LAN Configuration What You Have to Do First The module has an on board LAN communication interface 1 Note the MAC Media Access Control address for further reference The MAC or hardware address of the LAN interfaces is a world wide unique identifier No other network device will have the same hardware address The MAC address can be found on a label at the rear of the module underneath the configuration switch see Figure 56 on page 224 G4212 65800 Mga Part number of the detector main board B 01 XX 0925 Revision Code Vendor Year and Week of assembly MAC 000001000001 MAC address Made in Germany Country of Origin Figure55 MAC Label
60. IP address comment subnet mask for example in the Tab File If you change the IP address it will be necessary to power cycle the instrument for the changes to take effect Leave Edit BootP Addresses by pressing Close Exit BootP Settings by pressing OK Restarting the Agilent BootP Service 1 2 3 244 In the Windows control panel select Administrative Tools gt Services The Services screen appears see Figure 67 on page 243 Right click Agilent BootP Service and select Start Close the Services and Administrative Tools screens Agilent 1200 Infinity Series DAD User Manual LAN Configuration 13 Manual Configuration Manual configuration only alters the set of parameters stored in the non volatile memory of the module It never affects the currently active parameters Therefore manual configuration can be done at any time A power cycle is mandatory to make the stored parameters become the active parameters given that the initialization mode selection switches are allowing it Non Volatile RAM Stored Parameter Ld Control Module Figure 69 Manual Configuration Principle Agilent 1200 Infinity Series DAD User Manual 245 13 LAN Configuration With Telnet Whenever a TCP IP connection to the module is possible TCP IP parameters set by any method the parameters may be altered by opening a Telnet session 1 246 Open the system DOS prompt window by clicking on Windows STAR
61. LW 4 RT 2s Board Temperature a Figure 31 Detector Warm up 2 hour 108 Agilent 1200 Infinity Series DAD User Manual Agilent 1200 Infinity Series DAD User Manual 6 Troubleshooting and Diagnostics Overview of the Module s Indicators and Test Functions 110 Status Indicators 111 Power Supply Indicator 111 Module Status Indicator 112 Available Tests vs User Interfaces 113 Agilent Lab Advisor Software 114 Intermittent Issues 115 Loose Optical Cable 115 Board Type does not match when replacing the main board 116 Overview about the troubleshooting and diagnostic features Ee Agilent Technologies 109 6 Troubleshooting and Diagnostics Overview of the Module s Indicators and Test Functions Status Indicators The module is provided with two status indicators which indicate the operational state prerun run and error states of the module The status indicators provide a quick visual check of the operation of the module Error Messages In the event of an electronic mechanical or hydraulic failure the module generates an error message in the user interface For each message a short description of the failure a list of probable causes of the problem and a list of suggested actions to fix the problem are provided see chapter Error Information Test Functions A series of test functions are available for troubleshooting and operational verification after exchanging internal components see Test
62. Lamp 12 Max Light Cartridge Flow Cell 13 Slit Assembly 14 Grating and Diode Array 16 Bio inert Materials 17 This chapter gives an introduction to the detector and an instrument overview ee Agilent Technologies 9 1 Introduction Overview of the Module 10 The detector is designed for highest optical performance GLP compliance and easy maintenance It includes the following features Maximum of 160 Hz G4212A or 80 Hz G4212B data acquisition rate Higher sensitivity for conventional LC as well as ultra fast applications by using next generation optical design Increased sensitivity with 60 mm Max Light cartridge flow cell Optimized cell geometry for less peak dispersion for narrow bore applications Max Light cartridge flow cells for standard and bio inert applications are available More reliable and robust peak integration process automated due to less baseline noise drift refractive index and thermal effects especially under ultra fast gradient conditions RFID tracking technology is used for the UV lamp and the Max Light cartridge flow cells Multiple wavelength and full spectral detection at 160 Hz G4212A 80 Hz G4212B sampling rate keeping up with the analysis speed of ultra fast LC Programmable 1 8 nm slit G4212A or fixed 4 nm slit G4212B for rapid optimization of sensitivity linearity and spectral resolution provides optimum incident light conditions Improved Electronic temperature cont
63. Logging BootP Log File ESS ad Sa REG Default Settings Subnet mask GO 0 0 0 Gateway GO 0 0 0 Cancel Help Figure 64 BootP Settings screen 12 In the Default Settings part of the screen if known you can enter the subnet mask and gateway Defaults can be used The default subnet mask is 255 255 255 0 The default gateway is 192 168 254 11 13 On the BootP Settings screen click OK The Agilent BootP Service Setup screen indicates completion 14 Click Finish to exit the Agilent BootP Service Setup screen 15 Remove the DVD from the drive This completes installation 16 Start BootP Service in the Windows services On the Windows desktop click right on Computer icon select Manage gt Services and Applications gt Services Select the Agilent BootP Service and click Start 238 Agilent 1200 Infinity Series DAD User Manual LAN Configuration 13 Two Methods to Determine the MAC Address Enabling logging to discover the MAC address using BootP If you want to see the MAC address select the Do you want to log BootP requests check box 1 Open BootP Settings from Start gt All Programs gt Agilent BootP Service gt EditBootPSettings 2 In BootP Settings check Do you want to log BootP requests to enable logging Logging V Do you want to log bootP requests BootP Log File EEE EEE ee Figure 65 Enable BootP logging The log file is located in C Documents and Settings All Users
64. M The processor periodically checks if the calibration data are valid If the data are invalid or cannot be read from the spectrometer ROM the error message is generated Probable cause 1 Defective connector or cable 2 Defective PDA optical unit Suggested actions Please contact your Agilent service representative Please contact your Agilent service representative Agilent 1200 Infinity Series DAD User Manual Error Information 7 Wavelength Recalibration Lost Error ID 1037 The calibration information needed for your detector to operate correctly has been lost During calibration of the detector the calibration values are stored in ROM If no data is available in the spectrometer ROM the error message is generated Probable cause Suggested actions 1 The detector is new Recalibrate the detector 2 The detector has been repaired Please contact your Agilent service representative Illegal Temperature Value from Sensor on Main Board Error ID 1071 This temperature sensor located on the detector main board delivered a value outside the allowed range The parameter of this event equals the measured temperature in 1 100 centigrade As a result the temperature control is switched off Probable cause Suggested actions 1 Defective sensor or main board Please contact your Agilent service representative 2 Detector is exposed to illegal ambient Verify that the ambient conditions are within conditions the allowed r
65. Pressure Relief Valve Kit G4212 68001 190 This chapter provides information on parts for maintenance ott Agilent Technologies 187 10 Parts and Materials for Maintenance Overview of Maintenance Parts 188 BIO Linert For bio inert modules use bio inert parts only Item 9 10 11 12 p n 5190 0917 G4212 60008 G5615 60018 G4212 60007 G5615 60017 G4212 60032 G4212 60038 G4212 60011 5067 4660 5062 2462 5067 4691 5041 8388 5041 8389 5063 6527 G4212 40027 G4208A Description Long life Deuterium lamp 8 pin with RFID tag Max Light Cartridge Cell 10 mm V o 1 0 uL Max Light Cartridge Cell Bio inert 10 mm V o 1 0 pL includes Peek Capillary 1 5 mi d 0 18 mm 0890 1763 and PEEK Fittings 10 PK 5063 6591 Max Light Cartridge Cell 60 mm V o 4 0 uL Max Light Cartridge Cell Bio inert 60 mm V o 4 0 pL includes Peek Capillary 1 5 m i d 0 18 mm 0890 1763 and PEEK Fittings 10 PK 5063 6591 HDR Max Light Cartridge Cell 3 7 mm V o 0 4 pL ULD Max Light Cartridge Cell 10 mm V o 0 6 pL Max Light Cartridge Test Cell Inlet Capillary SST 0 12 mm I D 220 mm long PTFE Tubing flexible i d 0 8 mm o d 1 6 mm 2 m re order 5 m flow cell to waste Front Panel DAD VWD FLD 1260 1290 Leak funnel Leak funnel holder Tubing assembly i d 6 mm o d 9 mm 1 2 m to waste Leak downpipe Instant Pilot Instant Pilot G4208A requires firmware B 02 11 or above For cables refer
66. R ON H All modules connected to system are switched on Receiver is any module relying on operation of others 7 READY H System is ready for next analysis Receiver is any sequence controller 8 STOP L Request to reach system ready state as soon as possible for example stop run abort or finish and stop injection Receiver is any module performing run time controlled activities 9 START REQUEST L Request to start injection cycle for example by start key on any module Receiver is the autosampler Special Interfaces There is no special interface for this module 216 Agilent 1200 Infinity Series DAD User Manual Hardware Information 12 Setting the 8 bit Configuration Switch The 8 bit configuration switch is located at the rear of the module Switch settings provide configuration parameters for LAN serial communication protocol and instrument specific initialization procedures All modules with on board LAN e Default is ALL switches DOWN best settings Bootp mode for LAN and 19200 baud 8 data bit 1 stop bit with no parity for RS 232 For specific LAN modes switches 3 8 must be set as required e For boot test modes switches 1 2 must be UP plus required mode For normal operation use the default best settings AN CONFIG CAN CAN i USB o k O oof o HH D Agilent Tecdnsioges Figure 54 Location of Configuration Switch example shows a G4212A DAD Agi
67. STM 24 26 noise and linearity 28 programmable slit width 24 26 safety and maintenance 25 27 signal datarate 24 26 spectra data rate 24 26 wavelength accuracy 24 26 wavelength bunching 24 26 wavelength range 24 26 spectra acquisition 94 spectrum settings using 61 stack configuration front view 37 rearview 38 status indicator 112 suppression quantifying 96 system setup and installation optimizing stack configuration 32 system 11 T TCP IP parameter configuration 225 telnet configuration 246 temperature sensor 124 test cell usage 135 test functions 110 test function astm drift 148 celltest 143 conditions 136 D A converter 159 DAC 159 dark current 161 failing 137 intensity test 140 introduction 134 max light cartridge 135 noise 148 quick noise test 145 self test 138 slit 151 test cell cartridge 135 wavelength calibration 156 wavelength verification 154 time constant versus response time 28 time table settings using 64 timeout 119 troubleshooting Agilent 1200 Infinity Series DAD User Manual error messages 110 118 bunching 24 26 status indicators 110 111 range 24 26 WEEE directive 266 U weight 23 unpacking 30 user interfaces 113 using advanced method parameter settings 60 control settings 56 detector 50 general method settings 58 GUI 53 Instant Pilot 69 instrument configuration 67 instrument curves 66 method parameter settings 5 7 other advanced method parameter 63 spectr
68. T button and select Run Type cmd and press OK Type the following at the system DOS prompt c gt telnet lt IP address gt or c gt telnet lt host name gt ev C WINDOWS system32 cmd exe Cz gt telnet 134 40 30 205 Figure 70 Telnet Starting a session where lt IP address gt may be the assigned address from a Bootp cycle a configuration session with the Handheld Controller or the default IP address see Configuration Switch on page 226 When the connection was established successfully the module responds with the following 2A PROO10OALS Figure 71 A connection to the module is made Type and press enter to see the available commands cv Telnet 134 40 30 205 x Agilent Technologies G4212A PR 0100015 gt command syntax description display help info ay t LAN settings Figure 72 Telnet Commands Agilent 1200 Infinity Series DAD User Manual LAN Configuration 13 Table 27 Telnet Commands Value Description displays syntax and descriptions of commands displays current LAN settings ip lt X X X x gt sets new ip address sm lt X X X X gt sets new subnet mask gw lt X X X x gt sets new default gateway exit exits shell and saves all changes 4 To change a parameter follows the style parameter value for example ip 134 40 28 56 Then press Enter where parameter refers to the configuration parameter you are defining and value refers to the defi
69. U The higher the value the greater the baseline noise Set this value only if you expect negative absorbance greater than 100 mAU Slit G4212A You can select the optical bandwidth 1 2 4 or 8 nm of the detector the narrower the slit the smaller the optical bandwidth of the instrument but the lower its sensitivity The 1260 Infinity DAD G4212B has a fixed slit width of The smaller the optical bandwidth the higher the spectral Aira resolution Autobalance Defines whether a balance is performed prior to a run and or after a run has finished Lamp on required for analysis If unchecked the lamp will be turned off after the analysis has finished Agilent 1200 Infinity Series DAD User Manual 63 4 Using the Module Setting up the Detector with Agilent ChemStation Time Table Settings Timetable window 64 You may set up time events to change functions with their parameters over the run time Add lines as required Time Limits 0 00 to 99999 00 minutes in steps of 0 01 min Via the buttons in the bottom area time table lines can be added removed cut copied pasted or completely cleared You may set up time events to change functions with their parameters over the run time Add lines as required Limits 0 00 to 99999 00 minutes in steps of 0 01 min Based on the chosen function a certain parameter can be selected Agilent 1200 Infinity Series DAD User Manual Setting up the Detector with Ag
70. Use the Auto Configuration to define the LAN communication between the Agilent ChemStation and the host module usually the Agilent detector Changing parameters become active after reboot of the IP address 192 168 254 11 ChemStation C Hostname Agilent 1100 1200 1290 Series LE Auto Ge Device name based on the module Type ID based on the module product number Some modules may allow changing the type based on Device name EENE hardware firmware This results in a change of features and functions Serial number based on the module Firmware revision based on the module Firmware revision 8 06 23 0003 Options lists installed options TypeID G4212A Serial number PROO100015 Connection settings 68 Agilent 1200 Infinity Series DAD User Manual Using the Module 4 Main Screens of the Detector with Agilent Instant Pilot G4208A Below the main screens for the use of the detector are shown Welcome Control a DAD ail TEO NIR I 64212A PROO100015 1 Configure 2Maintenance 3Diagnosis Method Sequence Status Logbook morea Welcome 1 System On Off 2 System Get Ready 3 System Clear Errors 4 DAD Balance 5 More 1 System Set Defaults 2 Controller Format USB 3 DAD Spectrum DAD G4212A PROO100015 te earl Method Sequence Status Logbook Morea Agilent 1200 Infinity Series DAD User Manual The Welcome
71. With a UV visible detector based on a diode array and the correct choice of a reference wavelength setting quantitative detection is possible To suppress caffeine the reference wavelength must be set to 282 nm At this wavelength caffeine shows exactly the same absorbance as at 222 nm When the absorbance values are subtracted from each another any indication of the presence of caffeine is eliminated In the same way hydrochlorothiazide can be suppressed if caffeine is to be quantified In this case the wavelength is set to 204 nm and the reference wavelength to 260 nm Figure 26 on page 97 shows the chromatographic results of the peak suppression technique The trade off for this procedure is a loss in sensitivity The sample signal decreases by the absorbance at the reference wavelength relative to the signal wavelength Sensitivity may be decreased by as much as 10 30 Hydrochlorothiazide Hydrochlorothiazide and caffeine and caffeine Wavelength 204 nm Wavelength 222 nm No reference No reference Hydrochlorothiazides Caffeine suppressed uppressed Wavelength 204 nm Wavelength 222 nm Reference 260 nm Reference 282 nm 6 2 8 1 8 1 2 6 2 8 1 2 1 2 Time min Time min Figure 26 Peak Suppression Using Reference Wavelength Agilent 1200 Infinity Series DAD User Manual 97 5 Optimizing the Detector Ratio Qualifiers for Selective Detection of Compound Classes Ratio qualifiers can be used where in a complex sample only one
72. air troubleshooting and monitoring functionality is included Agilent 1200 Infinity Series DAD User Manual Troubleshooting and Diagnostics 6 Intermittent Issues Loose Optical Cable Following intermittent problems may occur Baseline drift noise No peaks Lamp intensity test fails WL verification fails Other effects related to the optics In such a case check the cable connection between optical unit under the cover see Figure 33 on page 115 and the main board If this is loose intermittent or constant effects can be seen Do not start replacements optical or main board before above check Figure 33 Location of Cover at Optical Unit Agilent 1200 Infinity Series DAD User Manual 115 Troubleshooting and Diagnostics Board Type does not match when replacing the main board 116 When replacing a main board in the DAD it may wake up with the wrong type In this case it has been used by another person in another DAD before G4212B DAD boots as G4212A DAD If the Main board was used previously in a G4212A DAD The module stays in resident mode G4212A R and the RED error LED is on Use the latest Agilent Lab Advisor Connect to the DAD Open Service amp Diagnostics Run Board Check and Change Change the type to G4212B Enter the correct serial number as written on the module label Apply the changes Reboot the DAD if not done automatically G4212A DAD boots as G4212B DAD If the Main board was
73. al Test Functions and Calibration 8 Failing a Test If a test fails with the Max Light Cartridge Cell repeat the test with the Max Light Cartridge Test Cell and compare If the test fails also then start with proposed actions mentioned in the details of the tests Agilent 1200 Infinity Series DAD User Manual 137 8 Test Functions and Calibration Self Test When Parts required OR Preparations 138 The self test runs a series of individual tests described on the next pages and evaluates the results automatically The following tests are run Slit Test G4212A only Dark Current Test Intensity Test Wavelength Verification Test ASTM Noise Test a simplified version of the ASTM Drift and Noise Test without testing the Drift For complete detector check 1 1 Description Max Light Cartridge Cell filled with water Max Light Cartridge Test Cell Lamp must be on for at least 10 minutes For noise test a longer warm up time may be required gt 2 hours When using a Max Light Cartridge Cell a flow rate of 0 5 mL min with water is required Agilent 1200 Infinity Series DAD User Manual Test Functions and Calibration 8 Self Test 1 Run the Self Test with the recommended user interface for further information see Online Help of user interface okce ro vest renaty nRange 190 2201 30257 cons west rnenaty Range z1 350mm Selecomns iames irtensty n Range 351 500mm wai coats tone raen
74. al and electronic boards components of the module are placed This pack is then housed in a metal inner cabinet which is enclosed by a plastic external cabinet The advantages of this packaging technology are e virtual elimination of fixing screws bolts or ties reducing the number of components and increasing the speed of assembly disassembly e the plastic layers have air channels molded into them so that cooling air can be guided exactly to the required locations the plastic layers help cushion the electronic and mechanical parts from physical shock and e the metal inner cabinet shields the internal electronics from electromagnetic interference and also helps to reduce or eliminate radio frequency emissions from the instrument itself 220 Agilent 1200 Infinity Series DAD User Manual Hardware Information 12 Early Maintenance Feedback Maintenance requires the exchange of components which are subject to wear or stress Ideally the frequency at which components are exchanged should be based on the intensity of usage of the module and the analytical conditions and not on a predefined time interval The early maintenance feedback EMF feature monitors the usage of specific components in the instrument and provides feedback when the user selectable limits have been exceeded The visual feedback in the user interface provides an indication that maintenance procedures should be scheduled EMF Counters EMF counters increment with
75. al resolution sensitivity and Use narrow slit e g 1 nm if your analytes have narrow absorbance bands and linearity for high concentrations Use a wide slit e g 8 nm to detect very low concentrations Optimizing spectral acquisition DAD only Select spectra acquisition mode according to your needs see Spectrum Settings on page 61 Set the spectral wavelength range for colorless samples 190 400 nm is sufficient Set step to 4 nm for normal use set small step and slit width if high resolution of spectra with fine structure is wanted Agilent 1200 Infinity Series DAD User Manual 79 5 80 Optimizing the Detector Choosing a Flow Cell Several flavors of the Max Light Cartridge Flow Cell are available see Table 7 on page 80 Table 7 Specifications for Max Light Cartridge Flow Cells Cartridge Cells Max Light Cartridge Cell 10 mm V o 1 0 uL G4212 60008 Max Light Cartridge Cell Bio inert 10 mm V o 1 0 pL G5615 60018 Max Light Cartridge Cell 60 mm V o 4 0 pL G4212 60007 Max Light Cartridge Cell Bio inert 60 mm V o 4 0 pL G5615 60017 HDR Max Light Cartridge Cell 3 7 mm V o 0 4 uL G4212 60032 ULD Max Light Cartridge Cell 10 mm V o 0 6 pL G4212 60038 Max Light Cartridge Test Cell G4212 60011 Maximum 70 bar 1015 psi Maximum Operating Pressure MOP Pressure 150 bar 2175 psi Maximum Incidential Pressure MIP 2 pH range 1 0 12 5 solvent dependent 1 Ma
76. alled and once with the Max Light Cartridge Test Cell The resulting intensity ratio is a measure of the amount of light absorbed by the Max Light Cartridge flow cell The test can be used to check for dirty or contaminated flow cell windows When the test is started the 1 nm slit is moved into the light path automatically G4212A only On the G4212B the 4 nm fixed slit is used This test should be performed initially with a new detector flow cell The values should be kept for later reference comparison When In case of low intensity or noise and drift problem Parts required Description 1 Max Light Cartridge Cell filled with water 1 Max Light Cartridge Test Cell Preparations Lamp must be on for at least 10 minutes When using a Max Light Cartridge Cell a flow rate of 0 5 mL min with water is required 1 Run the Cell Test with the recommended user interface for further information see Online Help of user interface Test Name Cell Test Description The test compares the lamp intensity the Max Light Cell and the Max Light Test Cell The intensity ratio is an indicator of Module G4212A PR00100018 the amount of light absorbed by the flow cell Status Passed Start Time 7 14 2009 1 40 44 PM Stop Time 7 14 2009 1 41 46 PM Test Procedure Result Name Value Y 1 Check Prerequisites Cell Product Number 64212 60008 ye eles 3 Scan intensiy Spectum 4 Inve suppoted Cel 5 Scanirtensiy Spectum intensty iteorlwahow cel 15
77. an amplitude of 10 uV anda frequency of approximately 1 cycle 24 seconds is applied to the signal The amplitude of the square wave and the peak to peak noise are used to evaluate the DAC test If the analog detector signal is noisy or missing Lamp must be on for at least 10 minutes Connect integrator chart recorder or data system to the detector analog output Running the test with Agilent LabAdvisor 1 Run the D A Converter DAC Test for further information see Online Help of user interface Test Name DJA Converter Test Description The test switches a test signal to the analog output that can be measured using an integrator or strip chart recorder Module G4212A PRO0100015 Status Passed Start Time 7 9 2009 3 06 30 PM Stop Time 7 9 2009 3 06 53 PM BERRERRRRSRRERREEI Test Procedure 7 1 Check Prerequisites 7 2 Switch on Analog Output 7 3 Switch off Analog Output Figure47 D A Converter DAC Test Results Agilent 1200 Infinity Series DAD User Manual 159 8 Test Functions and Calibration ADC1 A ADC CHANNEL 76 49 76 468 76 555 o2 04 os Figure 48 Running the Test with Instant Pilot i 12 1 4 16 18 D A Converter DAC Test Example of Integrator Plot The test can be started via the command line 1 To start the test TEST DAC 1 Reply RA 00000 TEST DAC 1 2 To stop the test TEST DAC 0 Reply RA 00000 TEST DAC 0 Test Evaluation The noise on the step
78. and should be flushed out See also Cleaning of Max Light Cartridge Cell on page 178 When using the Max Light Cartridge Cell for tests calibrations it should be run at 0 5 mL min constant flow with water This assures that the light path is always flushed Below table gives an idea on the signal height variation of the Max Light Cartridge Cells compared to Max Light Cartridge Test Cell Table 11 Max Light Cartridge Cells compared to Max Light Cartridge Test Cell PartNumber Description Signal Height typical G4212 60011 Max Light Cartridge Test Cell 100 G4212 60008 Max Light Cartridge Cell 10 mmV 1 pL 100 G4212 60007 Max Light Cartridge Cell 60 mmV 4 pL 100 G5615 60018 Max Light Cartridge Cell Bio inert 10 mmV o 1pL 100 G5615 60017 Max Light Cartridge Cell Bio inert60 mmV o 4yL 100 G4212 60032 Max Light Cartridge Cell HDR 3 7 mm V o 0 4 pL 100 G4212 60017 Max Light Cartridge Cell ULD 10 mm V o 0 6 uL 100 Agilent 1200 Infinity Series DAD User Manual 135 8 Test Functions and Calibration Conditions of Detector The test usually should be performed with a detector turned on for at least one hour so that the temperature regulation of the optical unit is working not active during the first 30 minutes after turn on If the detector is on tests can be performed usually 10 minutes after the UV lamp has been turned on 136 Agilent 1200 Infinity Series DAD User Manu
79. ange Agilent 1200 Infinity Series DAD User Manual 129 7 Error Information Illegal Temperature Value from Sensor at Fan Assembly Error ID 1072 This temperature sensor located close to the fan delivered a value outside the allowed range The parameter of this event equals the measured temperature in 1 100 centigrade As a result the temperature control is switched off Probable cause 1 The temperature sensor is defect 2 Defective main board 3 Detector is exposed to illegal ambient conditions Heater at fan assembly failed Error ID 1073 Suggested actions Please contact your Agilent service representative Please contact your Agilent service representative Verify that the ambient conditions are within the allowed range Every time the deuterium lamp or the tungsten lamp DAD only is switched on or off a heater self test is performed If the test fails an error event is created As a result the temperature control is switched off Probable cause 1 Defective connector or cable 2 Defective heater 130 Suggested actions Please contact your Agilent service representative Please contact your Agilent service representative Agilent 1200 Infinity Series DAD User Manual Error Information 7 Heater Power At Limit Error ID 1074 The available power of the heater reached either the upper or lower limit This event is sent only once per run The parameter determines which limit has been hi
80. ant Pilot Analog detector signal tional optional EN i PA CAN Bus cable Thermo cable optional AC Power Figure 14 Recommended two stack configuration for 1290 Infinity with binary pump rear view 40 Agilent 1200 Infinity Series DAD User Manual Installing the Module 3 Installing the Detector Parts required Software required Preparations Description 1 Detector 1 Power cord 1 LAN cable cross over or twisted pair network cable Other cables see below and section Cable Overview on page 192 Instant Pilot and or one of the following software solutions for control and data evaluation with the appropriate revisions 1 Agilent ChemStation for LC B 04 02 DSP3 or above 2 EZChrome Elite 3 3 2 SP2 or above 3 MassHunter B 04 00 and B 03 01 SP2 or above Test and diagnostic software e Agilent LabAdvisor B 01 03 SP4 or above Locate bench space Provide power connections Unpack the module Module is partially energized when switched off as long as the power cord is plugged in Repair work at the module can lead to personal injuries e g shock hazard when the cover is opened and the module is connected to power gt Make sure that it is always possible to access the power plug gt Remove the power cable from the instrument before opening the cover gt Do not connect the power cable to the Instrument while the covers are removed Agilent 1200 Infinity Series D
81. anual Optimizing the Detector 5 Warm up of the Detector Give the optical unit enough time to warm up and stabilize gt 60 minutes The detector is temperature controlled After turn on of the detector it goes through a cycle of different states 0 to 0 5 minutes the heater control is OFF and the heater element runs at 0 duty cycle e 0 5 to 1 minutes the heater control is OFF and the heater element runs at 66 duty cycle This first minute is used as self test of the heater functionality 1 to 30 minutes the heater control is OFF and the heater element runs at 40 duty cycle e After 30 minutes the heater control is ON and is working with optimized parameters to get the optical unit into the optimal temperature window stabilized This cycle starts e when the detector is turned off on e when the lamp is turned off on to ensure that the temperature control operates in a defined control range The times to stabilize the baseline may vary from instrument to instrument and depends on the environment The example below was done under stable environmental conditions The figures below show the first two hours of a detector warm up phase The lamp was turned on immediately after turn on of the detector Agilent 1200 Infinity Series DAD User Manual 107 5 Optimizing the Detector Warm up of the Detector Baseline 230 4 360 100 SLW 4 RT 2s Figure 30 Detector Warm up 1 hour Baseline 230 4 360 100 S
82. any other equipment Agilent 1200 Infinity Series DAD User Manual 21 2 Site Requirements and Specifications Bench Space The module dimensions and weight see Table 2 on page 23 allow you to place the module on almost any desk or laboratory bench It needs an additional 2 5 cm 1 0 inches of space on either side and approximately 8 cm 8 1 inches in the rear for air circulation and electric connections If the bench shall carry a complete HPLC system make sure that the bench is designed to bear the weight of all modules The module should be operated in a horizontal position Environment CAUTION Your module will work within the specifications at ambient temperatures and relative humidity described in Table 2 on page 23 ASTM drift tests require a temperature change below 2 C hour 3 6 F hour over one hour period Our published drift specification refer also to Specifications on page 24 is based on these conditions Larger ambient temperature changes will result in larger drift Better drift performance depends on better control of the temperature fluctuations To realize the highest performance minimize the frequency and the amplitude of the temperature changes to below 1 C hour 1 8 F hour Turbulences around one minute or less can be ignored Condensation within the module Condensation will damage the system electronics gt Do not store ship or use your module under conditions where temperature flu
83. arameters immediately They are not stored to the non volatile memory of the module Therefore the parameters are lost with the next power cycle of the module Bootp D Server Figure 58 Bootp Principle Agilent 1200 Infinity Series DAD User Manual 227 13 LAN Configuration Bootp amp Store When Bootp amp Store is selected the parameters obtained from a Bootp Server become the active parameters immediately In addition they are stored to the non volatile memory of the module Thus after a power cycle they are still available This enables a kind of bootp once configuration of the module Example The user may not want to have a Bootp Server be active in his network all the time But on the other side he may not have any other configuration method than Bootp In this case he starts the Bootp Server temporarily powers on the module using the initialization mode Bootp amp Store waits for the Bootp cycle to be completed closes the Bootp Server and powers off the module Then he selects the initialization mode Using Stored and powers on the module again From now on he is able to establish the TCP IP connection to the module with the parameters obtained in that single Bootp cycle Bootp Active Server Parameter Non Volatile RAM Stored Parameter Figure 59 Bootp amp Store Principle Use the initialization mode Bootp amp Store carefully because writing to the non volatile memory takes time Therefo
84. ark current 161 271 Index data collection rate 105 defect on arrival 30 delay volume 100 delivery checklist 31 detection compound classes 98 detector warmup 107 detector achieving higher sensitivity 101 installation 41 preparation 50 setup with ChemStation 51 DHCP general information 231 setup 232 dimensions 23 diode width 24 26 drift ASTM and noise 24 26 E electrical connections descriptions of 207 electronic waste 266 EMF early maintenance feedback 221 environment 22 error messages compensation sensor open 122 compensation sensor short 123 diode current leakage 126 fan failed 123 heater failed 130 heater power at limit 131 ignition without cover 124 124 illegal temperature value from sensor at fan assembly 130 illegal value from sensor on main board 129 leak sensor open 122 272 leak sensor short 121 leak 124 lost CAN partner 121 remote timeout 120 shutdown 120 timeout 119 uv heater current 128 uv ignition failed 127 uv lamp current 126 uv lamp voltage 127 wavelength calibration failed 128 wavelength recalibration lost 129 extra column volume description 100 F fan failed 123 features safety and maintenance 25 27 firmware description 204 main system 204 resident system 204 update tool 205 updates 205 183 upgrade downgrade 183 flow cell correction factors 86 Max Light cartridge flow cell 101 Max Light high sensitivity cell 101 solvent information 269 specification
85. ase provided with the Max Light Cartridge Flow Cell Agilent 1200 Infinity Series DAD User Manual 175 9 Maintenance Replacing the Max Light Cartridge Cell 9 Remove the black hoods from the cell interfaces in out 10 Slide the cell cartridge holder completely into the and insert the cell into the cell cartridge holder module 12 Remove the plugs from the CELL IN and CELL OUT keep cell them safe 176 Agilent 1200 Infinity Series DAD User Manual Maintenance 9 Replacing the Max Light Cartridge Cell 13 Connect the inlet capillary to CELL IN left and the waste tubing to CELL OUT right Agilent 1200 Infinity Series DAD User Manual 14 Close the front cover 177 9 Maintenance Cleaning of Max Light Cartridge Cell When Low counts on Intensity Test or Cell Test failed tests Tools required p n Description Alcohol lso propanol or Ethanol Lens tissue or Q tips 5062 8529 Cell cleaning fluid 1 L 1 Flush the flow cell with the alcohol for some time 2 Remove the cell from the cartridge holder see Replacing the Max Light Cartridge Cell on page 173 3 Carefully clean the light inlet and outlet of the cell using lens tissue or Q tips with alcohol If Q tips are used ensure that no cotton fluff remains at the inlet or outlet Do not touch the light inlet and outlet of the cell with your fingers This will add a layer of contamination
86. ata Agilent BootP LogFile The logfile will not be updated if it is open The contents will be similar to the following 02 25 10 15 30 49 PM Status BootP Request received at outermost layer Status BootP Request received from hardware address 0010835675AC Error Hardware address not found in BootPTAB 0010835675AC Status BootP Request finished processing at outermost layer 3 Record the hardware MAC address for example 0010835675AC Agilent 1200 Infinity Series DAD User Manual LAN Configuration 13 4 The Error means the MAC address has not been assigned an IP address and the Tab File does not have this entry The MAC address is saved to the Tab File when an IP address is assigned 5 Close the log file before turning on another instrument 6 Uncheck the Do you want to log BootP requests box after configuring instruments to avoid having the logfile use up excessive disk space Adding each instrument to the network using BootP 1 Follow Start gt All Programs gt Agilent BootP Service and select Edit BootP Settings The BootP Settings screen appears 2 Uncheck the Do you want to log BootP requests once all instruments have been added The Do you want to log BootP requests box must be unchecked when you have finished configuring instruments otherwise the log file will quickly fill up disk space 3 Click Edit BootP Addresses The Edit BootP Addresses screen appears 4 Click Add The Add BootP Entry screen app
87. ata files with all spectra may become a burden The detector provides functions to reduce the amount of data yet retaining the relevant spectral information For spectra options see Spectrum Settings on page 61 Range Only the wavelength range where the compounds in your sample absorb contains information that is useful for purity checks and library searches Reducing the spectrum storage range saves disk space Step Most substances have broad absorbance bands Display of spectra peak purity and library search works best if a spectrum contains 5 to 10 data points per width of the absorbance bands For anisic acid the example used before a step of 4 nm would be sufficient However a step of 2 nm gives a more optimal display of the spectrum Threshold Sets the peak detector Only spectra from peaks higher than threshold will be stored when a peak controlled storage mode is selected Agilent 1200 Infinity Series DAD User Manual Optimizing the Detector 5 Margin for Negative Absorbance The detector adjusts its gain during balance such that the baseline may drift slightly negative about 100 mAU In some special case for example when gradient with absorbing solvents are used the baseline may drift to more negative values Only for such cases increase the margin for negative absorbance to avoid overflow of the analog to digital converter Agilent 1200 Infinity Series DAD User Manual 95 5 Optimizing the Detector
88. ation provided with the firmware update tools The firmware update tools firmware and documentation are available from the Agilent web http www chem agilent com _layouts agilent downloadFirmware aspx whid 69761 Agilent 1200 Infinity Series DAD User Manual Hardware Information 12 Electrical Connections The CAN bus is a serial bus with high speed data transfer The two connectors for the CAN bus are used for internal module data transfer and synchronization e One analog output provides signals for integrators or data handling systems The REMOTE connector may be used in combination with other analytical instruments from Agilent Technologies if you want to use features such as start stop common shut down prepare and so on With the appropriate software the RS 232C connector may be used to control the module from a computer through a RS 232C connection This connector is activated and can be configured with the configuration switch The power input socket accepts a line voltage of 100 240 VAC 10 with a line frequency of 50 or 60 Hz Maximum power consumption varies by module There is no voltage selector on your module because the power supply has wide ranging capability There are no externally accessible fuses because automatic electronic fuses are implemented in the power supply Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety o
89. aty Range 9 GaOrm E est itenaty Range 190 350 iSc Figure 34 Self Test Results Agilent 1200 Infinity Series DAD User Manual 139 8 Test Functions and Calibration Intensity Test When Parts required OR Preparations 140 The intensity test measures the intensity of the UV lamp over the full wavelength range 190 640 nm Four spectral ranges are used to evaluate the intensity spectrum The test is used to determine the performance of the lamp and optics see also Cell Test on page 143 When the test is started the 1 nm slit is moved into the light path automatically G4212A only On the G4212B the 4 nm fixed slit is used To eliminate effects due to absorbing solvents the test should be done with water in the Max Light Cartridge Cell or with the Max Light Cartridge Test Cell The shape of the intensity spectrum is primarily dependent on the lamp grating and diode array characteristics Therefore intensity spectra will differ slightly between instruments In case of UV lamp problem drift noise Description 1 Max Light Cartridge Cell filled with water 1 Max Light Cartridge Test Cell Lamp must be on for at least 10 minutes Agilent 1200 Infinity Series DAD User Manual Test Functions and Calibration 8 Intensity Test 1 Run the Intensity Test with the recommended user interface for further information see Online Help of user interface Intensity Test Description The test scans the Int
90. ber of determinations It is important that the reference bandwidth does not extend to a part of the spectrum that has some absorbance as this would then reduce the resulting signal and sensitivity would be reduced The use of a reference wavelength can help to reduce drift or wander in the chromatogram caused by refractive index changes due to room temperature fluctuation or gradient operation The effect of a reference signal can be easily tested by setting two otherwise identical signals one with and one without a reference signal If there is no part of the spectrum with zero absorbance then it will be better to have the reference signal turned off Agilent 1200 Infinity Series DAD User Manual 103 5 Optimizing the Detector signal ini wavelength Anisic Acid reference bandwidth 10 Onm 30 nm reference bandwidth wavelength 350 nm 280 388 Wavelength Figure 29 Spectrum of Anisic Acid 104 Agilent 1200 Infinity Series DAD User Manual Optimizing the Detector 5 Peak Width Response Time and Data Collection Rate The peak width setting response time and data rate in the detector are all linked The available settings are shown in Table 9 on page 106 It is important to set this correctly for optimum sensitivity and to preserve the resolution achieved in the separation The detector internally acquires data points faster than is needed for a chromatogram and processes them to produce the signal seen by the data syst
91. ctuations could cause condensation within the module gt If your module was shipped in cold weather leave it in its box and allow it to warm slowly to room temperature to avoid condensation 22 This module is designed to operate in a typical electromagnetic environment i e where RF transmitters such as mobile telephones may not be used in close proximity Agilent 1200 Infinity Series DAD User Manual Physical Specifications Site Requirements and Specifications 2 Table 2 Physical Specifications Type Specification Comments Weight 11 5 kg 26 Ibs Dimensions 140 x 345 x 435 mm height x width x depth 5 5 x 13 5 x 17 inches Line voltage 100 240 VAC 10 Wide ranging capability Line frequency 50 or 60 Hz 5 Power consumption 160 VA 130 W 444 BTU Maximum Ambient operating temperature Ambient non operating temperature Humidity Operating altitude Non operating altitude Safety standards IEC CSA UL 4 40 C 39 104 F 40 70 C 40 158 F lt 80 rh at 40 C 104 F Up to 2000 m 6562 ft Up to 4600 m 15091 ft Installation category II Pollution degree 2 Non condensing For storing the module For indoor use only Agilent 1200 Infinity Series DAD User Manual 23 2 Site Requirements and Specifications Performance Specifications Specifications Performance Specifications G4212A Table3 Performance Specifications G4212A Type Specif
92. dditional peak broadening might occur The upper limit of concentration Care should be taken in methods where high background absorption of solvents or modifiers are present When using the 60 mm cell the detector will measure 6 times the background absorption as in case of the 10 mm cell which will reduce the remaining dynamic absorbance range for sample peaks Furthermore those UV absorbing modifiers could compromise the sensitivity gain signal noise of 60 mm cell The linearity limit of the detector is seen at about 2 AU for both the 10 mm and the 60 mm Max Light Cartridge Flow Cell Using firmware revision B 06 25 and below the 60 mm Max Light Cartridge Cell linearity limit would be 333 mAU cm Required Detector Firmware For use of the 60 mm Max Light Cartridge Flow Cell a detector firmware B 06 26 introduced December 2009 or later is required If the 60 mm Max Light Cartridge Flow Cell is used with detector firmware B 06 25 and below the detector output digital and analog is normalized to 1 cm This means the peak height would be the same as on the 10 mm Cartridge Flow Cell the noise is reduced by a factor of 6 and the linearity limit would be 333 mAU cm Agilent 1200 Infinity Series DAD User Manual 83 5 84 Optimizing the Detector Special Information for Bio Inert Cartridge Flow Cells Special Information for Bio Inert Cartridge Flow Cells For bio inert applications use the specified BIO Max Light Cartridge Flow
93. e bio molecules in the sample leading to sample degradation and contamination gt For bio inert applications always use dedicated bio inert parts which can be identified by the bio inert symbol or other markers described in this manual gt Do not mix bio inert and non inert modules or parts in a bio inert system The flow cell is shipped with a filling of isopropanol This is to avoid breakage due to subambient conditions In case the flow cell is not used for some time stored then flush the flow cell with iso propanol Agilent 1200 Infinity Series DAD User Manual 173 9 Maintenance Replacing the Max Light Cartridge Cell 1 Remove the front cover 2 Locate the cell area 3 Disconnect the inlet capillary to CELL IN left and the 4 Unlock the cell cartridge holder by pulling the lever to the waste tubing to CELL OUT right front 174 Agilent 1200 Infinity Series DAD User Manual Maintenance 9 Replacing the Max Light Cartridge Cell 5 The lever should be in the final down position 6 Pull the cell cartridge holder completely out towards the front 8 Replace the black hoods A to the cell interfaces in out and insert plugs B for save storage NOTE The hoods and the plugs should be always in place to protect the flow cell For longer storage the flow cell should be flushed and filled with isopropanol to prevent the grow of algae Store it in the plastic c
94. ears Add BootP Entry x Figure 66 Enable BootP logging Agilent 1200 Infinity Series DAD User Manual 241 13 LAN Configuration 5 Make these entries for the instrument e MAC address Host name Enter a Hostname of your choice The Host Name must begin with alpha characters i e LC1260 IP address e Comment optional Subnet mask e Gateway address optional The configuration information entered is saved in the Tab File Click OK Leave Edit BootP Addresses by pressing Close Exit BootP Settings by pressing OK oo N OO After each modification of the BootP settings i e EditBootPSettings a stop or start of the BootP service is required for the BootP service to accept changes See Stopping the Agilent BootP Service on page 243 or Restarting the Agilent BootP Service on page 244 10 Power cycle the Instrument OR If you changed the IP address power cycle the instrument for the changes to take effect 11 Use the PING utility to verify connectivity by opening a command window and typing Ping 192 168 254 1 1 for example The Tab File is located at C Documents and Settings All Users Application Data Agilent BootP TabFile 242 Agilent 1200 Infinity Series DAD User Manual LAN Configuration 13 Changing the IP Address of an Instrument Using the Agilent BootP Service Agilent BootP Service starts automatically when your PC reboots To change Agilent BootP Service settings you must stop the
95. ector of the instrument can be easily reached and unplugged gt Provide sufficient space behind the power socket of the instrument to unplug the cable 20 Agilent 1200 Infinity Series DAD User Manual Site Requirements and Specifications 2 Power Cords Different power cords are offered as options with the module The female end of all power cords is identical It plugs into the power input socket at the rear The male end of each power cord is different and designed to match the wall socket of a particular country or region Absence of ground connection or use of unspecified power cord The absence of ground connection or the use of unspecified power cord can lead to electric shock or short circuit gt Never operate your instrumentation from a power outlet that has no ground connection gt Never use a power cord other than the Agilent Technologies power cord designed for your region Use of unsupplied cables Using cables not supplied by Agilent Technologies can lead to damage of the electronic components or personal injury gt Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations Unintended use of supplied power cords Using power cords for unintended purposes can lead to personal injury or damage of electronic equipment gt Never use the power cords that Agilent Technologies supplies with this instrument for
96. el corrosive solvents Solutions of alkali halides and their respective acids for example lithium iodide potassium chloride and so on High concentrations of inorganic acids like sulfuric acid and nitric acid especially at higher temperatures if your chromatography method allows replace by phosphoric acid or phosphate buffer which are less corrosive against stainless steel Halogenated solvents or mixtures which form radicals and or acids for example 2CHCls Os gt 2COCly 2HCI This reaction in which stainless steel probably acts as a catalyst occurs quickly with dried chloroform if the drying process removes the stabilizing alcohol Chromatographic grade ethers which can contain peroxides for example THF dioxane di isopropylether such ethers should be filtered through dry aluminium oxide which adsorbs the peroxides Solvents containing strong complexing agents e g EDTA Mixtures of carbon tetrachloride with 2 propanol or THF Agilent 1200 Infinity Series DAD User Manual 269 14 Appendix Agilent Technologies on Internet For the latest information on products and services visit our worldwide web site on the Internet at http www agilent com 270 Agilent 1200 Infinity Series DAD User Manual Index 8 bit configuration switch on board LAN 217 A accessory kit parts 190 accessory kit 31 advanced method parameter settings using 60 Agilent Lab Advisor software 114 Agilent Lab Advisor 114 Agi
97. ell RFID tag 185 overview 16 7 replacing firmware 183 replacing leak handling system 181 replacing the deuterium lamp 169 replacing the flow cell 173 serial number and firmware revision 185 manual configuration of LAN 245 materials bio inert 17 message diode current leakage 126 heater failed 130 heater power at limit 131 ignition without cover 124 124 illegal temperature value from sensor atfan assembly 130 illegal value from sensor on main board 129 remote timeout 120 uv heater current 128 uv ignition failed 127 uv lamp current 126 uv lamp voltage 127 wavelength calibration failed 128 method parameter settings using 5 7 negative absorbance 95 noise and drift ASTM 24 26 Noise and Linearity specifications 28 noise 148 non operating altitude 23 non operating temperature 23 0 operating Altitude 23 operating temperature 23 optical 11 Agilent 1200 Infinity Series DAD User Manual Index optimization detector performance 78 detector sensitivity 101 for sensitivity selectivity linearity dispersion 85 how to get the best performance 78 margins for negative absorbance 95 of selectivity 96 overview 79 peak width 87 performance 77 sample and reference wavelength 89 slit width 92 spectra acquisition 94 stack configuration 32 wavelength and bandwidth 102 optimizing 1260 System 100 flow cell 80 other advanced method parameter using 63 overpressure relieve valve kit 82 overview
98. em Part of the processing reduces the data to an appropriate data rate which allows the chromatographic peaks to be accurately drawn As with most analytical determinations groups of readings are effectively averaged to reduce error in the result The detector bunches raw data points and produces the output signal data at the required data collection rate by an electronic filtering process If the resulting data rate is too slow over filtering the peak heights will be reduced and the resolution between them reduced too fast and the data is noisier than it need be to accurately profile narrow peaks The peak width setting in the detector allows the user to correctly set these parameters without needing any knowledge other than sight of the chromatogram integration results to see how wide the peaks are The peak width setting should be set for the narrowest peak width observed in the chromatogram If it is set too wide it will make the peaks appear lower in height and wider and potentially less resolved and if it is set too narrow it will increase the baseline noise unnecessarily Essentially the software uses this value to set the data collection rate such that it collects enough data points over the narrowest peaks and it is aiming for 15 to 25 points across a peak The 1290 Infinity DAD can collect at a maximum 160 Hz if required which would allow enough data points to be collected over a peak that is only 0 1 s wide The response time setting is a
99. ensity spectrum generated by the UV G4212A PROO100015 Passed 71912009 2 14 09 PM 7912009 2 14 30 PM hes Itenstyn Range S01 e0 35651 Cois Figure 35 Intensity Test Results Figure 36 Intensity Test Signals Agilent 1200 Infinity Series DAD User Manual 141 142 Test Functions and Calibration Test Failed Intensity Test Evaluation Probable cause 1 Absorbing solvent or air bubble in flow cell 2 Incorrect calibration 3 Dirty or contaminated flow cell 4 Dirty or contaminated optical components 5 Old UV lamp 6 Defect optical unit Suggested actions Ensure the flow cell is filled with water and free from air bubbles Repeat test with Max Light Cartridge Test Cell and compare results Recalibrate and repeat the test Run the cell test If the test fails flush the flow cell See also Cleaning of Max Light Cartridge Cell on page 178 Please contact your Agilent service representative Exchange the UV lamp If the test fails with Max Light Cartridge Test Cell and new UV lamp please contact your Agilent service representative If only one range fails and the application does not require this range the lamp may not be changed Agilent 1200 Infinity Series DAD User Manual Test Functions and Calibration 8 Cell Test The cell test measures the intensity of the UV lamp over the full wavelength range 190 690 nm once with the Max Light Cartridge Cell inst
100. equired Description 1 Max Light Cartridge Cell filled with water OR 1 Max Light Cartridge Test Cell Preparations Detector and UV lamp must be on for at least 2 hours ASTM measurements based on specifications may require longer stabilization times When using a Max Light Cartridge Cell a flow rate of 0 5 mL min with water is required Agilent 1200 Infinity Series DAD User Manual 145 8 Test Functions and Calibration Quick Noise Test 1 Run the Quick Noise Test with the recommended user interface for further information see Online Help of user interface Quick Noise Test Description The test performs a quick Noise Evaluation without reference G4212A PR00100015 Passed 7 9 2009 2 03 53 PM 7 9 2009 2 09 10 PM C a Testa cette SCSC d OO fame tee fwisneontwe dS Sana tosevabe erm ooo mA _ _ Figure 39 Quick Noise Test Results Figure 40 Quick Noise Test Signals 146 Agilent 1200 Infinity Series DAD User Manual Test Failed Quick Noise Test Evaluation Probable cause 1 Insufficient lamp warm up time 2 Absorbing solvent or air bubble in flow cell 3 Dirty or contaminated flow cell 4 Old UV lamp Agilent 1200 Infinity Series DAD User Manual Test Functions and Calibration 8 Suggested actions Allow detector and UV lamp turned on for at least 2 hours Ensure the flow cell is filled with water and free from air bubbles Flush flow cell Clean the f
101. erium gas the lamp emits light over the 190 nm to approximately 800 nm wavelength range PQ 4 Figure 2 UV Lamp Agilent 1200 Infinity Series DAD User Manual Introduction 1 Max Light Cartridge Flow Cell p n G4212 60008 G4212 60007 G5615 60018 G5615 60017 64212 60032 64212 60038 G4212 60011 The detector allows easy access to flow cells via a cartridge A variety of optional flow cells can be inserted using the same quick simple mounting system Max Light Cartridge Flow Cells for standard and bio inert applications are available For testing of the detector a Max Light Cartridge Test Cell is available Description Max Light Cartridge Cell 10 mm V o 1 0 uL Max Light Cartridge Cell 60 mm V o 4 0 uL Max Light Cartridge Cell Bio inert 10 mm V o 1 0 pL includes Peek Capillary 1 5 m i d 0 18 mm 0890 1763 and PEEK Fittings 10 PK 5063 6591 Max Light Cartridge Cell Bio inert 60 mm V o 4 0 pL includes Peek Capillary 1 5 m i d 0 18 mm 0890 1763 and PEEK Fittings 10 PK 5063 6591 HDR Max Light Cartridge Cell 3 7 mm V o 0 4 pL ULD Max Light Cartridge Cell 10 mm V o 0 6 pL Max Light Cartridge Test Cell The optical principle of the Max Light Cartridge cell is based on opto fluidic waveguides Nearly 100 light transmission is achieved by utilizing total internal reflection in a non coated silica fiber Compromising refractive index and thermal effects are almost completely eliminated
102. es of the Agilent 1260 Infinity LC System in the following configuration See Figure 7 on page 33 and Figure 8 on page 34 This configuration optimizes the flow path for minimum delay volume and minimizes the bench space required il Solvent cabinet Vacuum degasser oD Pump P Q A 888 Instant Pilot Autosampler Column compartment Detector Figure 7 Recommended Stack Configuration for 1260 Infinity Front View Agilent 1200 Infinity Series DAD User Manual 33 3 Installing the Module Optimizing the Stack Configuration INU MAINI PURE TT Remote cable CAN Bus cable to Instant Pilot AC power CAN Bus cable Analog detector signal 1 or 2 outputs partarai LAN to LC ChemStation location depends on detector Figure 8 Recommended Stack Configuration for 1260 Infinity Rear View 34 Agilent 1200 Infinity Series DAD User Manual Installing the Module 3 Optimizing the Stack Configuration One Stack Configuration for Agilent 1290 Infinity LC Ensure optimum performance by installing the modules of the Agilent 1290 Infinity Binary LC System in the following configuration See Figure 9 on page 35 and Figure 10 on page 36 This configuration optimizes the flow path for minimum delay volume and minimizes the bench space required The Agilent 1290 Infinity Binary Pump should always be installed at the bottom of the stack
103. etected when solvent cools the leak sensor causing the leak sensor current to change within defined limits If the current falls outside the lower limit the error message is generated Probable cause Suggested actions 1 Leak sensor not connected to the main Please contact your Agilent service board representative 2 Defective leak sensor Please contact your Agilent service representative 3 Leak sensor incorrectly routed being Please contact your Agilent service pinched by a metal component representative Compensation Sensor Open Error ID 0081 The ambient compensation sensor NTC on the main board in the module has failed open circuit The resistance across the temperature compensation sensor NTC on the main board is dependent on ambient temperature The change in resistance is used by the leak circuit to compensate for ambient temperature changes If the resistance across the sensor increases above the upper limit the error message is generated Probable cause Suggested actions 1 Defective main board Please contact your Agilent service representative 122 Agilent 1200 Infinity Series DAD User Manual Error Information 7 Compensation Sensor Short Error ID 0080 The ambient compensation sensor NTC on the main board in the module has failed short circuit The resistance across the temperature compensation sensor NTC on the main board is dependent on ambient temperature The change in resistance is used by t
104. etector 50 Setting up the Detector with Agilent ChemStation 51 Main Screens of the Detector with Agilent Instant Pilot G4208A 69 Solvent Information 73 5 Optimizing the Detector 77 Introduction 78 Optimization Overview 79 Optimizing for Sensitivity Selectivity Linearity and Dispersion 85 Optimizing Selectivity 96 Optimizing the Detector Regarding to the System 100 Warm up of the Detector 107 Agilent 1200 Infinity Series DAD User Manual Contents Troubleshooting and Diagnostics 109 Overview of the Module s Indicators and Test Functions 110 Status Indicators 111 Available Tests vs User Interfaces 113 Agilent Lab Advisor Software 114 Intermittent Issues 115 Board Type does not match when replacing the mainboard 116 Error Information 117 What Are Error Messages 118 General Error Messages 119 Detector Error Messages 126 Test Functions and Calibration 133 Introduction 134 Use of Max Light Cartridge Test Cell 135 Conditions of Detector 136 Failing a Test 137 Self Test 138 Intensity Test 140 Cell Test 143 Quick Noise Test 145 ASTM Drift and Noise Test 148 Slit Test 151 Wavelength Verification Test 154 Wavelength Calibration 156 D A Converter DAC Test 159 Dark Current Test 161 Maintenance 163 Warnings and Cautions 164 Introduction to Maintenance 166 Overview of Maintenance 167 Cleaning the Module 168 Replacing the Deuterium Lamp 169 Replacing the Max Light Cartridge Cell 173 Cleaning of Ma
105. etergent WARNING Liquid dripping into the electronic compartment of your module can cause shock hazard and damage the module gt Do not use an excessively damp cloth during cleaning gt Drain all solvent lines before opening any connections in the flow path 168 Agilent 1200 Infinity Series DAD User Manual Maintenance 9 Replacing the Deuterium Lamp When Tools required Parts required Preparations If noise or drift exceeds application limits or lamp does not ignite Description Screwdriver POZI 1 PT3 p n Description 1 5190 0917 Long life Deuterium lamp 8 pin with RFID tag Turn the lamp off Injury by touching hot lamp If the detector has been in use the lamp may be hot gt If so wait for lamp to cool down 1 Remove the front cover 2 Locate the lamp area Agilent 1200 Infinity Series DAD User Manual 169 Maintenance Replacing the 3 Unscrew the 3 screws of the lamp housing cover and remove the cover 4 Locate the two screws that fix the lamp and unscrew mer Xe Gaz12 60008 tw yA Agilent sart zt Agilent O 4212 60008 SO men ie 10 at inn Mews SFT Do not touch the glass bulb with your fingers It may reduce the light output 170 Agilent 1200 Infinity Series DAD User Manual Maintenance 9 aci g the Deuteriun 7 Insert the lamp and reconnect the lamp connector 8 Loca
106. exception e Is the LAN Interface using the BootP mode instead of Using Stored or Using Default modes Agilent 1200 Infinity Series DAD User Manual LAN Configuration 13 Installation of BootP Service Before installing and configuring the Agilent BootP Service be sure to have the IP addresses of the computer and instruments on hand 1 2 3 Log on as Administrator or other user with Administrator privileges Close all Windows programs Insert the Agilent ChemStation software DVD into the drive If the setup program starts automatically click Cancel to stop it 4 Open Windows Explorer 5 Go to the BootP directory on the Agilent ChemStation DVD and double click BootPPackage msi If necessary click the Agilent BootP Service icon in the task bar The Welcome screen of the Agilent BootP Service Setup Wizard appears Click Next The End User License Agreement screen appears Read the terms indicate acceptance then click Next The Destination Folder selection screen appears Install BootP to the default folder or click Browse to choose another location Click Next The default location for installation is C Program Files Agilent BootPService 10 Click Install to begin installation Agilent 1200 Infinity Series DAD User Manual 237 13 LAN Configuration 11 Files load when finished the BootP Settings screen appears BootP Settings a BootP Tab File Create Tab File Edit BootP Addresses
107. f a system In an Agilent 1260 Infinity Liquid Chromatograph the detector is located below a G1316 TCC on the bench In an Agilent 1290 Infinity Liquid Chromatograph the detector is located between a G1316 TCC below and the Solvent Compartment above See Optimizing the Stack Configuration on page 32 1 Remove the front cover 2 Remove the black hoods from the cell interfaces in out and store them in the plastic case provided with the Max Light Cartridge Flow Cell 44 Agilent 1200 Infinity Series DAD User Manual Installing the Module 3 Flow Connections to the Detector 3 Locate the flow cell cartridge 4 Unlock the flow cell cartridge by pulling the lever to the front 5 The lever should be in the final down position 6 Pull the flow cell cartridge completely out towards the front Agilent 1200 Infinity Series DAD User Manual 45 3 Installing the Module Flow Connections to the Detector 7 Remove the black hoods from the cell interfaces in out 8 Slide the cell cartridge holder completely into the and insert the cell into the cell cartridge holder module 10 Remove the plugs from the CELL IN and CELL OUT keep cell them safe 46 Agilent 1200 Infinity Series DAD User Manual Installing the Module 3 Flow Connections to the Detector 11 Connect the inlet capillary to CELL IN left and the waste 12 Close the front cover tubin
108. for Broad band universal detection Narrow band selective detection e Sensitivity for a specific analyte Broad band or universal detection works by having a wide bandwidth to detect any species with absorbance in that range For example to detect all absorbing molecules between 200 nm and 300 nm set a signal at 250 nm witha bandwidth of 100 nm The disadvantage is that sensitivity will not be optimal for any one of those molecules Narrow band or selective detection is used most often The UV spectrum for a particular molecule is examined and an appropriate absorbance maximum is selected If possible the range where solvents absorb strongly should be avoided below 220 nm for methanol below 210 nm for acetonitrile For example in Figure 29 on page 104 anisic acid has a suitable absorbance maximum at 252 nm A narrow bandwidth of 4 nm to 12 nm generally gives good sensitivity and is specific for absorbance in a narrow range The narrow band can be optimized for sensitivity for a specific molecule As the bandwidth is increased the signal is reduced but so is the noise and there Agilent 1200 Infinity Series DAD User Manual Optimizing the Detector 5 will be an optimum for best S N As an approximate guide this optimum is often close to the natural bandwidth at half height of the absorption band in the UV spectrum In the anisic acid example this is 30 nm The analytical wavelength is usually set at a wavelength maximum to increase
109. from the G1316C TCC or an optional DAD heat exchanger if available Peakwidth enables you to select the peak width response time for your analysis The peak width is defined as the width of a peak in minutes at half the peak height Set the peak width to the narrowest expected peak in your chromatogram The peak width sets the optimum response time for your detector The peak detector ignores any peaks that are considerably narrower or wider than the peak width setting The response time is the time between 10 and 90 of the output signal in response to an input step function When the All spectrum storage option is selected then spectra are acquired continuously depending on the setting of the peak width The time specified by the peak width is used as a factor in the acquisition of spectra The acquisition time for one spectrum is slightly less than the peak width divided by 8 which is the acquisition time Limits When you set the peak width in minutes the corresponding response time is set automatically and the appropriate data rate for signal and spectra acquisition is selected Do not use peak width shorter than necessary G4212B Do not use 0 025 seconds response time no filtering high noise and no need actually ultra fast LC doesn t deliver peaks lt 0 0025 min lt 0 15 sec The 1290 Infinity DAD G4212A has a data rate of up to 160 Hz The 1260 Infinity DAD G4212B has a data rate of up to 80 Hz Agi
110. g a Max Light Cartridge Cell a flow rate of 0 5 mL min with water is required 154 Agilent 1200 Infinity Series DAD User Manual Test Functions and Calibration 8 Wavelength Verification Test 1 Run the Wavelength Verification Test with the recommended user interface for further information see Online Help of user interface Test Name Wavelength Verification Test Description The test performs a Wavelength Verification Module G4212A DEBAF00917 1290 DAD Status Passed Start Time 6 26 2012 10 41 15 AM Stop Time 6 26 2012 10 41 39 AM Test Procedure Check Prerequisites Accumulated UV Lamp Burn Time Insert Test Cell UV Lamp On Time 4 2 3 Wavelength Verification 4 RRRS Evaluate Data D2 Alpha Line Deviation NL Calibration Limit for Alpha Line 0 D2 Beta Line Deviation 0 179 nm WL Calibration Limit for Beta Line l 0 Figure 45 Wavelength Verification Results Agilent 1200 Infinity Series DAD User Manual 155 8 Test Functions and Calibration Wavelength Calibration When Parts required Preparations 156 The detector uses the alpha 656 1 nm and beta 486 nm emission lines of the deuterium lamp for wavelength calibration The sharp emission lines enable more accurate calibration than is possible with holmium oxide When recalibration is started the 1 nm slit is moved into the light path automatically G4212A The gain is set to zero On completion of the
111. g to CELL OUT right 13 Route the inlet capillary and waste tubing towards their destinations NOTE The detector should be operated with the front cover in place to protect the flow cell area against strong drafts from the outside NOTE If the flow cell is replaced by a different flow cell it should be flushed with isopropanol and the CELL IN and CELL OUT should be closed with the plugs NOTE To protect the flow cell against overpressure please refer to Inline Pressure Relief Valve Kit G4212 68001 on page 82 Agilent 1200 Infinity Series DAD User Manual 47 3 Installing the Module Initial Recalibration The detector has been calibrated with a factory flow cell initially After installation of the detector with the delivered or a new Max Light Cartridge flow cell and an initial warm up of at least 2 hours a recalibration should be performed Wavelength Calibration on page 156 This recalibration will correct slight changes due to e significant environmental condition changes temperature humidity during transport and storage e significant environmental condition changes temperature humidity in final location and variances between factory test cell and the installed flow cell 48 Agilent 1200 Infinity Series DAD User Manual Agilent 1200 Infinity Series DAD User Manual ee 4 Using the Module e Preparing the Detector 50 Setting up the Detector with Agilent ChemSta
112. ge Cell 10 mm V o 1 0 pL G4212 60008 70 bar 1015 psi Maximum Local Control Test and troubleshooting software Analog outputs Communications Safety and maintenance GLP features Housing Max Light Cartridge Cell 60 mm V o 4 0 pL G4212 60007 HDR Max Light Cartridge Cell 3 7 mm V o 0 4 pL G4212 60032 ULD Max Light Cartridge Cell 10 mm V o 0 6 pL G4212 60038 Max Light Cartridge Cell Bio inert 10 mm V o 1 0 uL G5615 60018 Max Light Cartridge Cell Bio inert 60 mm V o 4 0 uL G5615 60017 Max Light Cartridge Test Cell G4212 60011 Agilent Instant Pilot G4208A Agilent LabAdvisor Recorder integrator 100 mV or 1 V output range 0 001 2 AU one output Controller area network CAN RS 232C APG Remote ready start stop and shut down signals LAN Extensive support for troubleshooting and maintenance is provided by the Instant Pilot Agilent Lab Advisor and the Chromatography Data System Safety related features are leak detection safe leak handling leak output signal for shutdown of pumping system and low voltages in major maintenance areas Early maintenance feedback EMF for continuous tracking of instrument usage in terms of lamp burn time with user setable limits and feedback messages Electronic records of maintenance and errors Verification of wavelength accuracy with the emission lines of the deuterium lamp All materials recyclable Operating Pressure Mop
113. gth band with the width of reference bandwidth The signals comprises a series of data points over time with the average absorbance in the sample wavelength band minus the average absorbance of the reference wavelength band Signal A in the detector default method is set to sample 254 0 4 reference 360 0 100 that is the average absorbance from 252 256 nm minus the average absorbance from 310 410 nm As all analytes show higher absorbance at 252 256 nm than at 310 410 nm this signal will show you virtually every compound which can be detected by UV absorbance Many compounds show absorbance bands in the spectrum Figure 20 on page 90 shows the spectrum of anisic acid as an example To optimize for lowest possible detectable concentrations of anisic acid set the sample wavelength to the peak of the absorbance band that is 252 nm and the sample bandwidth to the width of the absorbance band that is 30 nm A reference of 360 100 is adequate Anisic acid does not absorb in this range If you work with high concentrations you may get better linearity above 1 5 AU by setting the sample wavelength to a valley in the spectrum like 225 nm for anisic acid Agilent 1200 Infinity Series DAD User Manual 89 5 Optimizing the Detector cas pS gt sample wavelength 252 nm Anisic acid lt 2 Reference bandwidth 100 nm 5 T 38 o o 224 7 30 nm 2 bandwidth Pd Reference wavelength 360 i 4 220 248 260 280 300 320
114. h range for spectral storage Limits 190 to 640 nm in steps of 1 nm for both low and high values The high value must be greater than the low value by at least 2 nm 61 4 Using the Module Step Threshold 62 Step defines the wavelength resolution for spectral storage Limits 0 10 to 100 00 nm nm in steps of 0 1 nm The threshold is the height in mAU of the smallest expected peak The peak detector ignores any peaks which are lower than the threshold value and does not save spectra Limits 0 001 to 1000 00 mAU in steps of 0 001 mAU Agilent 1200 Infinity Series DAD User Manual Using the Module 4 Other Advanced Method Parameter Settings Analog Output This screen part of the Advanced Method Settings soa shows the default settings Zero Offset 5 x Altenuation 1000 mA Maton for negative Absorbance Sih 100 mAU 4 vj na Autobalance Lamps on required for aquisition J Pieun UVLamp Posteun Analog Output The Range can be set to either 100 mV or 1 V full scale see Control Settings on page 56 Zero Offset 1 to 99 in steps of 1 5 equal to 50 mV Attenuation 0 98 to 2000 mAU at discrete values for either 100 mV or 1 V full scale Margin for Negative Absorbance Use this field to modify the detector s signal handling to increase the margin for negative absorbance Use this option if for example your solvent gradient produces a decreasing baseline absorbance and for GPC analyses Limits 100 to 4000 mA
115. he leak circuit to compensate for ambient temperature changes If the resistance across the sensor falls below the lower limit the error message is generated Probable cause Suggested actions 1 Defective main board Please contact your Agilent service representative Fan Failed Error ID 0068 The cooling fan in the module has failed The hall sensor on the fan shaft is used by the main board to monitor the fan speed If the fan speed falls below a certain limit for a certain length of time the error message is generated Depending on the module assemblies e g the lamp in the detector are turned off to assure that the module does not overheat inside Probable cause Suggested actions 1 Fan cable disconnected Please contact your Agilent service representative 2 Defective fan Please contact your Agilent service representative 3 Defective main board Please contact your Agilent service representative Agilent 1200 Infinity Series DAD User Manual 123 7 Error Information Leak Error ID 0064 A leak was detected in the module The signals from the two temperature sensors leak sensor and board mounted temperature compensation sensor are used by the leak algorithm to determine whether a leak is present When a leak occurs the leak sensor is cooled by the solvent This changes the resistance of the leak sensor which is sensed by the leak sensor circuit on the main board Probable cause Suggested actions 1 Loose fi
116. he system s critical parts in case any module detects a serious problem To detect whether all participating modules are switched on or properly powered one line is defined to summarize the POWER ON state of all connected modules Control of analysis is maintained by signal readiness READY for next analysis followed by START of run and optional STOP of run triggered on the respective lines In addition PREPARE and START REQUEST may be issued The signal levels are defined as e standard TTL levels 0 V is logic true 5 0 V is false fan out is 10 e input load is 2 2 kOhm against 5 0 V and e output are open collector type inputs outputs wired or technique All common TTL circuits operate with a 5 V power supply A TTL signal is defined as low or L when between 0 V and 0 8 V and high or H when between 2 0 V and 5 0 V with respect to the ground terminal Agilent 1200 Infinity Series DAD User Manual 215 12 Hardware Information Table 17 Remote Signal Distribution Pin Signal Description 1 DGND Digital ground 2 PREPARE L Request to prepare for analysis for example calibration detector lamp on Receiver is any module performing pre analysis activities 3 START L Request to start run timetable Receiver is any module performing run time controlled activities 4 SHUT DOWN L System has serious problem for example leak stops pump Receiver is any module capable to reduce safety risk 5 Not used 6 POWE
117. hecklist Description Quantity Detector Power cable Cross over network cable Twisted pair network cable 1 1 1 1 Max Light Cartridge Cell as ordered 1 User Manual Accessory kit on Documentation CD part of the shipment not module specific 1 Detector Accessory Kit Contents Detector Accessory Kit Contents p n G4212 68755 p n 5062 2462 5063 6527 5042 9967 0100 1516 5067 4660 5181 1516 Agilent 1200 Infinity Series DAD User Manual Description PTFE Tubing flexible i d 0 8 mm o d 1 6 mm 2 m re order 5 m flow cell to waste Tubing assembly i d 6 mm o d 9 mm 1 2 m to waste Tubing clip set of 5 clips Fitting male PEEK 2 pk Inlet Capillary SST 0 12 mm I D 220 mm long CAN cable Agilent module to module 0 5 m 31 3 Installing the Module Optimizing the Stack Configuration If your module is part of a complete Agilent 1260 Infinity 1290 Infinity LC System you can ensure optimum performance by installing the following configurations These configurations optimize the system flow path ensuring minimum delay volume For other possible configurations please refer to the Agilent 1260 Infinity 1290 Infinity LC System Manual 32 Agilent 1200 Infinity Series DAD User Manual Installing the Module 3 Optimizing the Stack Configuration One Stack Configuration One Stack Configuration for Agilent 1260 Infinity LC Ensure optimum performance by installing the modul
118. hen the ratio between 249 and 224 nm was within this range is the signal plotted Of all four peaks only the third fulfilled the criterion Figure 28 on page 99 The others were not plotted Agilent 1200 Infinity Series DAD User Manual 99 5 Optimizing the Detector Optimizing the Detector Regarding to the System 100 Delay Volume and Extra Column Volume The delay volume is defined as the system volume between the point of mixing in the pump and the top of the column The extra column volume is defined as the volume between the injection point and the detection point excluding the volume in the column Extra Column Volume Extra column volume is a source of peak dispersion that will reduce the resolution of the separation and so should be minimized Smaller diameter columns require proportionally smaller extra column volumes to keep peak dispersion at a minimum In a liquid chromatograph the extra column volume will depend on the connection tubing between the autosampler column and detector and on the volume of the flow cell in the detector The extra column volume is minimized with the Agilent 1290 Infinity Agilent 1260 Infinity LC system due to the narrow bore 0 12 mm i d tubing the low volume heat exchangers in the column compartment and the Max Light cartridge cell in the detector How to Configure the Optimum Delay Volume To maintain resolution in the Agilent 1290 Infinity Agilent 1260 Infinity Diode array Detect
119. here each combination unambiguously denotes a module there can be more than one code for the same module Serial number 209 12 Hardware Information Interfaces The Agilent 1200 Infinity Series modules provide the following interfaces Table 15 Agilent 1200 Infinity Series Interfaces Module CAN LAN BCD LAN RS 232 Analog APG Special optional on board Remote Pumps G1310B Iso Pump 2 Yes No Yes 1 Yes G1311B Quat Pump G1311C Quat Pump VL G1312B Bin Pump K1312B Bin Pump Clinical Ed G1312C Bin Pump VL 1376A Cap Pump G2226A Nano Pump G5611A Bio inert Quat Pump G4220A B Bin Pump 2 No Yes Yes No Yes CAN DC OUT for G4204A Quat Pump CAN slaves G1361A Prep Pump 2 Yes No Yes No Yes CAN DC OUT for CAN slaves Samplers G1329B ALS 2 Yes No Yes No Yes THERMOSTAT for G2260A Prep ALS G1330B K1330B G1364B FC PS 2 Yes No Yes No Yes THERMOSTAT for G1364C FC AS G1330B K1330B G1364D FC uS CAN DC OUT for G1367E HiP ALS CAN slaves K1367E HiP ALS Clinical Ed G1377A HiP micro ALS G2258A DL ALS G5664A Bio inert FC AS G5667A Bio inert Autosampler G4226A ALS 2 Yes No Yes No Yes 210 Agilent 1200 Infinity Series DAD User Manual Hardware Information 12 Table 15 Agilent 1200 Infinity Series Interfaces Module CAN LAN BCD LAN RS 232 Analog APG Special optional on board Remote Detectors G1314B VWD VL 2 Yes No Yes 1 Yes G1314C VWD VL G1314E F VWD 2 No Yes Yes 1 Yes K1314F Clinical Ed G4212A B DAD 2
120. here it has been observed a leak always causes a shutdown of all modules even outside a method run In all cases error propagation is done via the CAN bus or via an APG remote cable see documentation for the APG interface Agilent 1200 Infinity Series DAD User Manual Error Information 7 General Error Messages General error messages are generic to all Agilent series HPLC modules and may show up on other modules as well Timeout Error ID 0062 The timeout threshold was exceeded Probable cause Suggested actions 1 The analysis was completed successfully Check the logbook for the occurrence and and the timeout function switched off the source of a not ready condition Restart the module as requested analysis where required 2 Anot ready condition was present duringa Check the logbook for the occurrence and sequence or multiple injection run for a source of a not ready condition Restart the period longer than the timeout threshold analysis where required Agilent 1200 Infinity Series DAD User Manual 119 7 120 Error Information Shutdown Error ID 0063 An external instrument has generated a shutdown signal on the remote line The module continually monitors the remote input connectors for status signals A LOW signal input on pin 4 of the remote connector generates the error message Probable cause 1 Leak detected in another module with a CAN connection to the system 2 Leak detected in an external
121. hould be green The module is turned on when the line power switch is pressed and the green indicator lamp is illuminated The module is turned off when the line power switch is protruding and the green light is off Agilent 1200 Infinity Series DAD User Manual Installing the Module 3 The module was shipped with default configuration settings To change these settings see chapter LAN Configuration After turn on of the detector it goes through a cycle of different states of heating up the optical unit and controlling the temperature This is described in Warm up of the Detector on page 107 Give the optical unit enough time to warm up and stabilize gt 60 minutes Agilent 1200 Infinity Series DAD User Manual 43 3 Installing the Module Flow Connections to the Detector FW For bio inert modules use bio inert parts only Parts required Description 1 System 1 Max Light cartridge flow cell 1 Capillaries and tubing from Accessory Kit CAUTION Sample degradation and contamination of the instrument Metal parts in the flow path can interact with the bio molecules in the sample leading to sample degradation and contamination gt For bio inert applications always use dedicated bio inert parts which can be identified by the bio inert symbol or other markers described in this manual gt Do not mix bio inert and non inert modules or parts in a bio inert system This procedure shows the detector outside o
122. ication Comments Detection type Light source Wavelength range Short term noise ASTM Single and Multi Wavelength Drift Linear absorbance range Wavelength accuracy Wavelength bunching Slit width Diode width Signal data rate Spectra Data rate 1024 element photodiode array Deuterium lamp 190 640 nm lt 3 x 10 AU at 230 nm 4 nm with 10 mm Max Light cartridge cell Typically lt 0 6 x 10 AU cm at 230 nm 4 nm with 60 mm Max Light cartridge cell lt 0 5 x 10 AU hr at 230 nm gt 2 0 AU 5 at 265 nm 1nm 2 400 nm 1 2 4 8 nm 0 5 nm up to 160 Hz up to 160 Hz Equipped with RFID tag that holds lamp typical information see Specification Conditions below see Specification Conditions below see Specification Conditions below After recalibration with deuterium lines Programmable in steps of 1 nm Programmable slit 24 Agilent 1200 Infinity Series DAD User Manual Site Requirements and Specifications 2 Table3 Performance Specifications G4212A Type Specification Comments Flow cells Max Light Cartridge Cell 10 mm V o 1 0 pL G4212 60008 70 bar 1015 psi Maximum Local Control Test and troubleshooting software Analog outputs Communications Safety and maintenance GLP features Housing Max Light Cartridge Cell 60 mm V o 4 0 pL G4212 60007 HDR Max Light Cartridge Cell 3 7 mm V o 0 4 pL G4212 60032
123. ilent ChemStation Time Table Graph In this view the activated signals are shown how they change according the time table ES KS 3 3 3 3 C3 c3 4 4 Using the Module Setting up the Detector with Agilent ChemStation Instrument Curves The detector has several signals internal temperatures voltages of lamps that can be used for diagnosing problems These can be baseline problems deriving from deuterium lamps wander drift problems due to temperature changes These signals can be used in addition to the normal baseline signal to determine whether correlation to temperature or voltage current of the lamp These signals are available via the Agilent ChemStation Online Plot Data Signal and or Agilent Lab Advisor Software Setup Method 66 Agilent 1200 Infinity Series DAD User Manual Using the Module 4 Setting up the Detector with Agilent ChemStation Instrument Configuration These settings are available via menu Instrument Instrument Configuration instrument Configuration 11007120071290 Series LC mo 1100 1200 12390 Series LC Qas SH 040 1542124 Pronoms Dros te m GG ouspuro Of camino Figure 18 Menu Instrument Configuration Via the Instrument Configuration screen additional modules can be added to a system Agilent 1200 Infinity Series DAD User Manual 67 4 Using the Module Setting up the Detector with Agilent ChemStation
124. ion Setup 260 Agilent 1200 Infinity Series DAD User Manual Agilent 1200 Infinity Series DAD User Manual 14 Appendix Safety 262 The Waste Electrical and Electronic Equipment Directive 266 Radio Interference 267 Sound Emission 268 Solvent Information 269 Agilent Technologies on Internet 270 This chapter provides addition information on safety legal and web Apg Agilent Technologies 261 14 Appendix Safety Safety Symbols Table 28 Safety Symbols Symbol Description The apparatus is marked with this symbol when the user should refer to the instruction manual in order to protect risk of harm to the operator and to protect the apparatus against damage Indicates dangerous voltages Indicates a protected ground terminal Indicates eye damage may result from directly viewing the light produced by the deuterium lamp used in this product The apparatus is marked with this symbol when hot surfaces are available A and the user should not touch it when heated up WARNING ahaa alerts you to situations that could cause physical injury or death gt Do not proceed beyond a warning until you have fully understood and met the indicated conditions CAUTION A CAUTION alerts you to situations that could cause loss of data or damage of equipment gt Do not proceed beyond a caution until you have fully understood and met the indicated conditions 262 Agilent 1200 Infinity Series DAD User Manual
125. iption 1 Max Light Cartridge Test Cell or 1 Max Light Cartridge Cell Detector lamp must be on for more than 1 hour e When using a Max Light Cartridge Cell a flow rate of 0 5 mL min with water is required Agilent 1200 Infinity Series DAD User Manual Test Functions and Calibration 8 Wavelength Calibration If the detector is operated in a lab environment that differs at average from the final test environment 25 C then the detector should be recalibrated for this temperature NOTE NOTE If the detector was repaired opened covers the wavelength calibration can be done 10 minutes after lamp on A final wavelength calibration should be repeated after complete warm up of the detector 1 Run the Wavelength Calibration with the recommended user interface for further information see Online Help of user interface Test Name Wavelength Calibration Description The wavelength calibration procedure enables you to check the calibration of the diode array in the detector Module G4212A DEBAF00917 1290 DAD Calibration means adjusting the assignment of diodes to specific Status Diss pining rice y oe using the two deuterium emission lines at Start Time 6 26 2012 10 42 44 AM Stop Time 6 26 2012 10 43 13 AM Test Procedure Name Value vt ak Penia Y 2 isot Test Cal 3 Wavelength Verification mla deraan de eal P E ON Cell Product Number G4212 60011 i D2 Alpha Line Deviation 0 199 nm D2 Beta Line Deviation 0 183 nm
126. ity of the transmitted light I divided by the intensity of the incident light Io is the extinction coefficient which is a characteristic of a given substance under a precisely defined set of conditions of wavelength solvent temperature and other parameters C mol L is the concentration of the absorbing species and d cm is the path length of the cell used for the measurement The detector can now output the signal in two forms 1 In Absorbance divide by the path length AU cm that is then similar to x C Advantage samples with same concentration have same peak height also at cells with different path lengths The upper limit of concentration the linearity limit of the detector is then seen at about 2 AU path length so for the 6 cm Max Light Cartridge Cell the linearity limit is 333 mAU cm 2 In AU that is equal to x C x d like normal done in the past now for recalculation to your concentration C the path length must be considered Therefore flow cells with longer path lengths yield higher signals Although noise usually increases little with increasing path length there is a gain in signal to noise ratio When increasing the path length the cell volume could increase Depending on the peak volume this could cause more peak dispersion Agilent 1200 Infinity Series DAD User Manual 85 5 Optimizing the Detector As a rule of thumb the flow cell volume should be about 1 3 of the peak volume at half heigh
127. le to General Purpose p n 01046 60201 Wire Color Pin Agilent Signal Name Active module TTL 7 White 1 Digital ground a Ol ou Brown 2 Prepare run Low 00 pes DE KEY Gray 3 Start Low oo o0 S iE Blue 4 Shut down Low og Pink 5 Not oa connected 5 5 15 Q Yellow 6 Power on High Red 7 Ready High Green 8 Stop Low Black 9 Start request Low 198 Agilent 1200 Infinity Series DAD User Manual BCD Cables Identifying Cables 11 One end of these cables provides a 15 pin BCD connector to be connected to the Agilent modules The other end depends on the instrument to be connected to Agilent Module to General Purpose p n G1351 81600 Wire Color Pin Agilent Signal Name BCD Digit module Green 1 BCD 5 20 pea Violet 2 BCD 7 80 AA Blue 3 BCD 6 40 a ee Yellow 4 BCD 4 10 Black 5 BCD 0 1 Ras Orange 6 BCD 3 8 Red 7 BCD 2 4 Brown 8 BCD 1 2 Gray 9 Digital ground Gray Gray pink 10 BCD 11 800 Red blue 11 BCD 10 400 White green 12 BCD 9 200 Brown green 13 BCD 8 100 notconnected 14 notconnected 15 5V Low Agilent 1200 Infinity Series DAD User Manual 199 11 Identifying Cables Agilent Module to 3396 Integrators p n 03396 60560 Pin 3396 Pin Agilent Signal Name BCD Digit module an 1 1 BCD 5 20 2 2 BCD7 80 Se 15 oO Z 3 3 BCD 6 40 oO So 4 4 BCD 4 10 s je 2 5 5 BCDO 1 fie e9 6 6 BCD 3 8 7 7 BCD 2 4 8 8 BCD
128. lent ChemStation Setup 251 on internet 270 algae 269 269 ambient non operating temperature 23 ambient operating temperature 23 analog signal output 25 27 analog signal 214 analog cable 194 apg remote 215 array diode 16 astm drift 148 ASTM environmental conditions 22 automatic configuration with Bootp 235 bandwidth 103 BCD cable 199 Beer Lambert law 85 bench space 22 bio inert 44 173 materials 17 BootP service installation 237 restart 244 settings 243 stop 243 Bootp amp Store 228 automatic configuration 235 initialization modes 227 using default 229 using stored 229 C cable analog 194 BCD 199 CAN 201 connecting APG remote 38 connecting CAN 38 connecting LAN 38 connecting the ChemStation 38 connecting the power 38 LAN 201 remote 196 RS 232 202 cables analog 192 BCD 192 CAN 193 LAN 193 overview 192 Agilent 1200 Infinity Series DAD User Manual Index remote 192 RS 232 193 CAN cable 201 cartridge cleaning 178 max light 178 storage 179 cautions and warnings 164 celltest 143 ChemStation setup 254 cleaning flow cell 178 compensation sensor open 122 compensation sensor short 123 condensation 22 30 configuration switche 226 configuration one stack 33 33 35 stack 32 two stack front 39 two stack rear 40 two stack 37 39 control settings using 56 correction factors for flow cells 86 D D A converter 159 DAC Agilent LabAdvisor 159 Instant Pilot 160 d
129. lent 1200 Infinity Series DAD User Manual Peakwidth time programmed Peakwidth same as Peakwidth 7 2 times greater than Peakwidth 4 times greater than Peakwidth 8 times greater than Peakwidth Stoptime Posttime Stoptime Posttime As Pump Injector Off min min Agilent 1200 Infinity Series DAD User Manual Using the Module 4 These selections can be made during time programmed operation When used in a timetable Peakwidth changes the filters used for peak controlled spectra acquisition but not the data rate of a chromatographic signal This setting makes sense only with peak controlled spectra it allows you to change the peakwidth setting to account for broadening peaks at the end of the run The stoptime is the time where either the complete system stops As Pump Injector or the module if different from system stop time The data collection is stopped at this time A posttime period can be used to allow module s items to equilibrate e g after gradient change or temperature change 59 4 Using the Module Advanced Method Parameter Settings These settings are available via a click on the Advanced link of the Method Parameter Settings in case the Time Table Settings are open This screen shows the default settings Advanced a Spectrum Store None y fron t n d n Analog Output Output 1 Zero Offset S Attenuation 1000 mau Margin for negative Absorbance Slit 100 m u 4
130. lent 1200 Infinity Series DAD User Manual 217 12 Hardware Information To perform any LAN configuration SW1 and SW2 must be set to OFF For details on the LAN settings configuration refer to chapter LAN Configuration Table 18 8 bit Configuration Switch with on board LAN Mode Function SW 6 SW7 swt sw2 LAN o o Auto negotiation 10 MBit half duplex 10 MBit full duplex 100 MBit half duplex 100 MBit full duplex Bootp Bootp amp Store Using Stored DHCP Using Default TEST 1 1 Boot Resident System Revert to Default Data Coldstart SW 3 Link Configuration SW 4 X 0 0 SW 5 Init Mode Selecti X X SW 8 10n So S NVRAM Legend 0 switch down 1 switch up x any position When selecting the mode TEST the LAN settings are Auto Negotiation amp Using Stored For explanation of Boot Resident System and Revert to Default Data Coldstart refer to Special Settings on page 219 218 Agilent 1200 Infinity Series DAD User Manual Hardware Information 12 Special Settings The special settings are required for specific actions normally in a service case The tables include both settings for modules with on board LAN and without on board LAN They are identified as LAN and no LAN Boot Resident Firmware update procedures may require this mode in case of firmware loading errors main firmware part If you use the fol
131. lit 4 nm First serial number was DEAA301100 Agilent 1200 Infinity Series DAD User Manual 15 1 16 Introduction Optical System Grating and Diode Array The combination of dispersion and spectral imaging is accomplished by using a concave holographic grating The grating separates the light beam into all its component wavelengths and reflects the light onto the photodiode array The diode array is a series of 1024 individual photodiodes and control circuits located on a ceramic carrier It has a wavelength range from 190 640 nm and the sampling interval is 0 5 nm Figure 6 Grating and diode array Agilent 1200 Infinity Series DAD User Manual Introduction 1 Bio inert Materials For the Agilent 1260 Infinity Bio inert LC system Agilent Technologies uses highest quality materials in the flow path also referred to as wetted parts which are widely accepted by life scientists as they are known for optimum inertness to biological samples and ensure best compatibility with common samples and solvents over a wide pH range Explicitly the complete flow path is free of stainless steel and free of other alloys containing metals such as iron nickel cobalt chromium molybdenum or copper which can interfere with biological samples The flow downstream of the sample introduction contains no metals whatsoever Max Light Cartridge Cell Bio inert 60 mm V o 4 0 uL G5615 60017 and Max Light Cartridge Cell Bio inert 10 mm
132. low cell as described in Cleaning of Max Light Cartridge Cell on page 178 Exchange the UV lamp 147 8 Test Functions and Calibration ASTM Drift and Noise Test When Parts required OR Preparations 148 The ASTM noise test determines the detector noise over a period of 20 minutes The test is done with installed Max Light Cartridge Cell or Max Light Cartridge Test Cell This test does also check for the drift It is also part of the Self Test without checking for the drift If the test is performed with the Max Light Cartridge Test Cell the test results are not influenced by solvent or pump effects In case of noise and drift problem Description 1 Max Light Cartridge Cell filled with water 1 Max Light Cartridge Test Cell Detector and UV lamp must be on for at least 2 hours ASTM measurements based on specifications may require longer stabilization times When using a Max Light Cartridge Cell a flow rate of 0 5 mL min with water is required Agilent 1200 Infinity Series DAD User Manual Test Functions and Calibration 8 ASTM Drift and Noise Test 1 Run the ASTM Drift and Noise Test with the recommended user interface for further information see Online Help of user interface ASTM Drift and Noise Test Description The test performs ASTM Drift and Noise evaluation without G4212A PR00100015 es Passed 7 9 2009 3 52 03 PM 7 9 2009 4 12 26 PM Figure 41 ASTM Drift and Noise Test
133. lowing switch settings and power the instrument up again the instrument firmware stays in the resident mode It is not operable as a module It only uses basic functions of the operating system for example for communication In this mode the main firmware can be loaded using update utilities Table 19 Boot Resident Settings On board LAN Mode Select sw1 sw2 SW3 sSw4 SW5 SW6 SW7 SW8 TEST BOOT 1 1 1 0 0 0 0 0 Forced Cold Start A forced cold start can be used to bring the module into a defined mode with default parameter settings CAUTION Loss of data Forced cold start erases all methods and data stored in the non volatile memory Exceptions are calibration settings diagnosis and repair log books which will not be erased gt Save your methods and data before executing a forced cold start If you use the following switch settings and power the instrument up again a forced cold start has been completed Table 20 Forced Cold Start Settings On board LAN Mode Select sw1 sw2 SW3 sw4 SW5 SW6 SW7 sws TEST BOOT 1 1 0 0 0 0 0 1 Agilent 1200 Infinity Series DAD User Manual 219 12 Hardware Information Instrument Layout The industrial design of the module incorporates several innovative features It uses Agilent s E PAC concept for the packaging of electronics and mechanical assemblies This concept is based upon the use of expanded polypropylene EPP layers of foam plastic spacers in which the mechanic
134. mit 131 This chapter describes the meaning of error messages and provides information on probable causes and suggested actions how to recover from error conditions ote Agilent Technologies 117 7 Error Information What Are Error Messages 118 Error messages are displayed in the user interface when an electronic mechanical or hydraulic flow path failure occurs which requires attention before the analysis can be continued for example repair or exchange of consumables is necessary In the event of such a failure the red status indicator at the front of the module is switched on and an entry is written into the module logbook If an error occurs outside a method run other modules will not be informed about this error If it occurs within a method run all connected modules will get a notification all LEDs get red and the run will be stopped Depending on the module type this stop is implemented differently For example for a pump the flow will be stopped for safety reasons For a detector the lamp will stay on in order to avoid equilibration time Depending on the error type the next run can only be started if the error has been resolved for example liquid from a leak has been dried Errors for presumably single time events can be recovered by switching on the system in the user interface Special handling is done in case of a leak As a leak is a potential safety issue and may have occurred at a different module from w
135. most all common acids bases and solvents There are no documented incompatibilities for HPLC applications Sapphire Ruby and Al 03 based ceramics Sapphire ruby and ceramics based on Al Og are inert to almost all common acids bases and solvents There are no documented incompatibilities for HPLC applications Data above were collected from external resources and are meant as a reference Agilent cannot guarantee the completeness and correctness of such information Information can also not be generalized due to catalytic effects of impurities like metal ions complexing agents oxygen etc Most data available refers to room temperature typically 20 25 C 68 77 F If corrosion is possible it usually increases at higher temperatures If in doubt consult additional resources Agilent 1200 Infinity Series DAD User Manual 75 4 Using the Module Solvent Information 76 Agilent 1200 Infinity Series DAD User Manual Agilent 1200 Infinity Series DAD User Manual 5 Optimizing the Detector Introduction 78 Optimization Overview 79 Choosing a Flow Cell 80 Inline Pressure Relief Valve Kit G4212 68001 82 Application Information 82 Special Information of 60 mm Cartridge Flow Cell 83 Special Information for Bio Inert Cartridge Flow Cells 84 Optimizing for Sensitivity Selectivity Linearity and Dispersion 85 Flow Cell Path Length 85 Peak width response time 87 Sample and Reference Wavelength and Bandwidth 89 Slit Width G4212A
136. nection Properties 21x General Advanced Connect using B Broadcom NetXtreme Gigabit Etheme Configure This connection uses the following items Client for Microsoft Networks v 5 File and Printer Sharing for Microsoft Networks M E QoS Packet Scheduler Internet Protocol TCP IP Install Uninstall Description Transmission Control Protocol Intermet Protocol The default wide area network protocol that provides communication across diverse interconnected networks IV Show icon in notification area when connected IV Notify me when this connection has limited or no connectivity Agilent 1200 Infinity Series DAD User Manual 251 13 LAN Configuration PC and Agilent ChemStation Setup 2 You may enter here the fixed IP address of the module or use the Alternative Configuration i Internet Protocol TCP IP Properties 252 Agilent 1200 Infinity Series DAD User Manual LAN Configuration 13 PC and Agilent ChemStation Setup 3 We will use the direct LAN access via Cross over LAN cable with the module s IP address 1 TCP IP Properties 4 Click on OK to save the configuration Agilent 1200 Infinity Series DAD User Manual 253 13 LAN Configuration Agilent ChemStation Setup This procedure describes the Agilent ChemStation B 04 02 setup for the 1290 Infinity system using the 1290 Infinity DAD G4212A as the interfacing module The setup works in the same way for all
137. nitions you are assigning to that parameter Each parameter entry is followed by a carriage return Agilent 1200 Infinity Series DAD User Manual 247 13 LAN Configuration 5 Use the and press Enter to list the current settings cx Telnet 134 40 30 205 gt Init Mode TCP IP Propert TCP IP Statu Controllers gt no connections Figure 73 Telnet Current settings in Using Stored mode information about the LAN interface MAC address initialization mode Initialization mode is Using Stored active TCP IP settings TCP IP status here ready connected to PC with controller software e g Agilent ChemStation here not connected 6 Change the IP address in this example 192 168 254 12 and type to list current settings 255 6 134 46 24 1 Ready Controllers gt no connections Figure 74 Telnet Change IP settings 248 change of IP setting to Initialization mode is Using Stored active TCP IP settings stored TCP IP settings in non volatile memory connected to PC with controller software e g Agilent ChemStation here not connected Agilent 1200 Infinity Series DAD User Manual LAN Configuration 13 7 When you have finished typing the configuration parameters type exit and press Enter to exit with storing parameters cx C WINDOWS system32 cmd exe Agilent Technologies G4212A PRO61400615 gt exit Connection to host lost Ceo Figure 75 Closing the Telnet Sessi
138. njury are present in this instrument Use extreme caution when handling testing and adjusting When working with solvents observe appropriate safety procedures for example goggles safety gloves and protective clothing as described in the material handling and safety data sheet by the solvent vendor especially when toxic or hazardous solvents are used Agilent 1200 Infinity Series DAD User Manual 265 14 Appendix The Waste Electrical and Electronic Equipment Directive Abstract The Waste Electrical and Electronic Equipment WEEE Directive 2002 96 EC adopted by EU Commission on 13 February 2003 is introducing producer responsibility on all electric and electronic appliances starting with 13 August 2005 This product complies with the WEEE Directive 2002 96 EC marking requirements The affixed label indicates that you must not discard this electrical electronic product in domestic household waste Product Category With reference to the equipment types in the WEEE Directive Annex I this product is classed as a Monitoring and Control Instrumentation product Do not dispose off in domestic household waste To return unwanted products contact your local Agilent office or see www agilent com for more information 266 Agilent 1200 Infinity Series DAD User Manual Appendix 14 Radio Interference Cables supplied by Agilent Technologies are screened to provide optimized protection against radio interference All cable
139. nother way of indicating how this filtering is set It is measured in seconds and is about one third of the peak width value which is measured in minutes It effectively shows how quickly the plotted signal responds to a step change in the input signal The full spectra is not available under all conditions Based on the data points the scan data rate is reduced see Table 9 on page 106 Agilent 1200 Infinity Series DAD User Manual 105 5 Optimizing the Detector Table 9 Peak Width Response Time Data Rate Peak width athalf Response Signaldata Scan data rate Scan data rate Scan data rate Scan data rate height min s rate Hz HZ HZ HZ HZ 126 pts scan 251 pts scan 501 pts scan gt 501 pts scan lt 0 0016 0 016 1602 1602 80 40 20 gt 0 0016 0 03 1602 1602 80 40 20 gt 0 003 0 062 80 80 80 80 40 gt 0 006 0 12 40 40 40 40 40 gt 0 012 0 25 20 20 20 20 20 gt 0 025 0 5 10 10 10 10 10 gt 0 05 1 0 5 5 5 5 5 gt 0 10 2 0 2 5 2 5 2 5 2 5 2 5 gt 0 20 4 0 1 25 1 25 1 25 1 25 1 25 gt 0 40 8 0 0 625 0 62 0 625 0 625 0 625 gt 0 85 16 0 0 3125 0 31 0 3125 0 3125 0 3125 1 2 64212A only 106 Values in the User Interface may be rounded The maximum spectra scan rate depends on the data points per scan see Table 9 on page 106 Running at 160 Hz the spectra scan data rate is reduced automatically if the spectra scan data rate is more than 251 points scan Agilent 1200 Infinity Series DAD User M
140. nt with warranty terms covering the material in this document that conflict with these terms the warranty terms in the sep arate agreement shall control Technology Licenses The hardware and or software described in this document are furnished under a license and may be used or copied only in accor dance with the terms of such license Restricted Rights Legend If software is for use in the performance of a U S Government prime contract or subcon tract Software is delivered and licensed as Commercial computer software as defined in DFAR 252 227 7014 June 1995 or as a commercial item as defined in FAR 2 101 a or as Restricted computer soft ware as defined in FAR 52 227 19 June 1987 or any equivalent agency regulation or contract clause Use duplication or dis closure of Software is subject to Agilent Technologies standard commercial license terms and non DOD Departments and Agencies of the U S Government will receive no greater than Restricted Rights as defined in FAR 52 227 19 c 1 2 June 1987 U S Government users will receive no greater than Limited Rights as defined in FAR 52 227 14 June 1987 or DFAR 252 227 7015 b 2 November 1995 as applicable in any technical data Safety Notices CAUTION A CAUTION notice denotes a hazard It calls attention to an operating procedure practice or the like that if not correctly per formed or adhered to could result in damage to the p
141. ode receives light within a range of wavelength determined by the slit width This explains why the fine spectral structure of benzene disappears when using a 8 nm wide slit Furthermore the absorbance is no longer strictly linear with concentration for wavelengths at a steep slope of a compound s spectrum Substances with fine structures and steep slopes like benzene are very rare In most cases the width of absorbance bands in the spectrum is more like 30 nm as with anisic acid Figure 20 on page 90 In most situations a slit width of 4 nm will give the best results Use a narrow slit 1 or 2 nm if you want to identify compounds with fine spectral structures or if you need to quantify at high concentrations gt 1000 mAU with a wavelength at the slope of the spectrum Signals with a wide bandwidth can be used to reduce baseline noise Because digital bandwidth is computed as average of absorbance there is no impact on linearity Use a wide 8 nm slit when your sample contains very small concentrations Always use signals with bandwidth at least as wide as the slit width Agilent 1200 Infinity Series DAD User Manual 93 5 94 Optimizing the Detector Optimizing Spectral Acquisition Storage of all spectra consumes a lot of disk space It is very useful to have all spectra available during optimization of a method or when analyzing unique samples However when running many samples of the same type the large size of d
142. on If the Initialization Mode Switch is changed now to Using Stored mode the instrument will take the stored settings when the module is re booted In the example above it would be 192 168 254 12 Agilent 1200 Infinity Series DAD User Manual 249 13 LAN Configuration Manual Configuration With the Instant Pilot G4208A To configure the TCP IP parameters before connecting the module to the network the Instant Pilot G4208A can be used 1 From the Welcome screen press the More button 2 Select Configure 3 Press the DAD button 4 Scroll down to the LAN settings lt Not Set gt Use lamp tag settings 0V 1V_ output range Stays off at power on 40 28 56 LAN Subnet Mask 255 255 255 0 LAN Def Gateway _134 40 24 1 xX Cancel Valid from 0 to 255 RT 16 08 Figure 76 Instant Pilot LAN Configuration Edit mode 5 Press the Edit button only visible if not in Edit mode perform the required changes and press the Done button 6 Leave the screen by clicking Exit 250 Agilent 1200 Infinity Series DAD User Manual LAN Configuration 13 PC and Agilent ChemStation Setup PC Setup for Local Configuration This procedure describes the change of the TCP IP settings on your PC to match the module s default parameters in a local configuration see Table 23 on page 229 1 Open the Local Area Connection Properties and select Internet Protocol TCP IP Then click on Properties 4 Local Area Con
143. on the window and reduce the light throughput 4 Flush the flow cell with water and repeat the Intensity Test and or Cell Test 5 If tests fail again the flow cell might be replaced if the chromatographic performace cannot be accepted If the cleaning with the alcohol did not improve you may use Cell cleaning fluid 1 L 5062 8529 178 Agilent 1200 Infinity Series DAD User Manual Maintenance 9 Storage of Max Light Cartridge Cell 1 Flush the Max Light Cartridge Flow Cell with iso propanol or methanol and insert the plugs into the cell inlet and outlet see Replacing the Max Light Cartridge Cell on page 173 2 Remove the Max Light Cartridge Cell from the cartridge holder of the detector 3 Replace the black hoods that secure the cell light inlet and outlet 4 Store the Max Light Cartridge Cell in plastic case provided with the Max Light Cartridge Flow Cell Agilent 1200 Infinity Series DAD User Manual 179 9 Maintenance Drying the Leak Sensor Drying the Leak Sensor When If leak has occurred Tools required Description Tissue Preparations Turn the pump off 1 Remove the front cover 2 Locate the leak sensor area 3 Dry the leak sensor and the area around Check loose 4 Close the front cover fittings at the flow cell Note that the leak sensor does not touch the panel clearance of about 1 mm 180 Agilent 1200 Infinity Series DAD User Manual Maintenance 9 Replacing Leak Handling
144. onds the error message is generated Probable cause 1 Lamp too hot Hot gas discharge lamps may not ignite as easily as cold lamps 2 Lamp disconnected 3 Defective UV lamp or non Agilent lamp 4 Defective detector main board 5 Defective power supply Agilent 1200 Infinity Series DAD User Manual Suggested actions Switch off the lamp and allow it to cool down for at least 15 minutes Ensure the lamp is connected Exchange the UV lamp Please contact your Agilent service representative Please contact your Agilent service representative 127 Error Information UV Heater Current Error ID 7453 The UV lamp heater current is missing During UV lamp ignition the processor monitors the heater current If the current does not rise above the lower limit within one second the error message is generated Probable cause 1 Lamp disconnected 2 Ignition started without the top foam in place 3 Defective UV lamp or non Agilent lamp 4 Defective detector main board 5 Defective power supply Calibration Values Invalid Error ID 1036 Suggested actions Ensure the UV lamp is connected Please contact your Agilent service representative Exchange the UV lamp Please contact your Agilent service representative Please contact your Agilent service representative The calibration values read from the spectrometer ROM are invalid After recalibration the calibration values are stored in RO
145. onfiguration for 1260 Infinity Front View Agilent 1200 Infinity Series DAD User Manual 37 3 Installing the Module Optimizing the Stack Configuration LAN to control software RAN Bus cable to Instant Pilot utosampler Fraction Cu llector C TTT IN SI Remote cable Figure 12 Recommended Two Stack Configuration for 1260 Infinity Rear View 38 Agilent 1200 Infinity Series DAD User Manual Installing the Module 3 Optimizing the Stack Configuration Two Stack Configuration for Agilent 1290 Infinity LC In case the autosampler thermostat is added to the system a two stack configuration is recommended which places both heavy modules 1290 Infinity pump and thermostat at the bottom of each stack and avoids high stacks Some users prefer the lower height of this arrangement even without the autosampler thermostat A slightly longer capillary is required between the pump and autosampler See Figure 13 on page 39 and Figure 14 on page 40 Instant Pilot Detector Column compartment J l J 1 Solvent cabinet Pump Al ce ee j d q Autosampler Thermostat for the ALS optional Figure 13 Recommended two stack configuration for 1290 Infinity with binary pump front view Agilent 1200 Infinity Series DAD User Manual 39 3 Installing the Module Optimizing the Stack Configuration LAN to LC ChemStation CAN Bus cable to Inst
146. or the 10 mm Max Light cartridge cell has a low dispersion volume 6 volume 1 0 uL and no further volume optimization is required In situations where the alternative 60 mm Max Light high sensitivity cell is used to get higher sensitivity the cell volume is optimized for the use with 3 mm and 4 6 mm inner diameter columns Agilent 1200 Infinity Series DAD User Manual Optimizing the Detector 5 How to Achieve Higher Sensitivity The detector has a number of parameters that are used to optimize performance The following sections describe how the detector parameters affect performance characteristics e Flow cell affects sensitivity Wavelength and bandwidth affect sensitivity selectivity and linearity Slit Width affects sensitivity spectral resolution and linearity e Peak Width affects sensitivity and resolution Flow Cell The Max Light cartridge flow cell has a standard 10 mm path length and is optimized for minimal volume and dispersion o volume 1 0 uL It has high light transmission minimizing noise to reduce noise due to the optofluidic waveguide It is suitable for use with a wide range of analytical columns from short narrow bore columns to long standard diameter 4 6 mm columns Generally the peak dispersion volume calculated from peak width x flow rate should be greater than about 2 uL for this cell for example 0 02 min x 200 uL min 4 uL The Max Light high sensitivity cell has a path length of 60 mm and this will
147. otection has been impaired the instrument must be made inoperative and be secured against any intended operation Make sure that only fuses with the required rated current and of the specified type normal blow time delay and so on are used for replacement The use of repaired fuses and the short circuiting of fuse holders must be avoided Some adjustments described in the manual are made with power supplied to the instrument and protective covers removed Energy available at many points may if contacted result in personal injury Any adjustment maintenance and repair of the opened instrument under voltage should be avoided whenever possible When inevitable this has to be carried out by a skilled person who is aware of the hazard involved Do not attempt internal service or adjustment unless another person capable of rendering first aid and resuscitation is present Do not replace components with power cable connected Do not operate the instrument in the presence of flammable gases or fumes Operation of any electrical instrument in such an environment constitutes a definite safety hazard Do not install substitute parts or make any unauthorized modification to the instrument 264 Agilent 1200 Infinity Series DAD User Manual Appendix 14 Capacitors inside the instrument may still be charged even though the instrument has been disconnected from its source of supply Dangerous voltages capable of causing serious personal i
148. other systems The LAN must be connected to detector due to high data load on communication to Control Software 1 Open the ChemStation Configuration Editor 5 Configuration itor Lo File Configure Utilities Help Instrment 1 z LC 3D Data Analysis only 2 Select from the menu Configure Instruments 3 Select Modular 3D LC System 4 Give the Instrument a name 254 Agilent 1200 Infinity Series DAD User Manual LAN Configuration 13 PC and Agilent ChemStation Setup 5 Click on OK Select Instrument Initially Start Instrument Session Yes No tem Access Dual Channel Iface 359008 CTCAutoSampler CTC PAL AutoSampler Agilent 1200 Infinity Series DAD User Manual 255 13 LAN Configuration PC and Agilent ChemStation Setup 7 Click on OK The Configuration Editor shows now the new instrument 8 If required change under Configure Path the folder locations 9 Save the current configuration via File Save 5 Configuration editor o File Configure Utilities Help nstrment 1 4 Nodular 3D LC System LC System Access Access Point 10 Exit the Configuration Editor 11 Start the Agilent ChemStation During first startup or when the system configuration has changed a notification shows up 256 Agilent 1200 Infinity Series DAD User Manual LAN Configuration 13 PC and Agilent ChemStation Setup 12 The left column shows the modules that could be configured You ma
149. pressure typically around 100 bar it releases the pressure to waste Application Information For the analysis and characterization of proteins and large biomolecules for SEC AEX and RP applications add 100 mM salt into mobile phase or 10 organic to prevent secondary interaction For cation exchange chromatography the usage of an Agilent Diode Array Detector G1315C D with the respective bio inert flow cell is highly recommended to avoid unspecific interaction of the protein with the flow cell For applications with mobile phases of a pH above 12 5 use an Agilent Diode Array Detector G1315C D and the respective bio inert flow cell 82 Agilent 1200 Infinity Series DAD User Manual Optimizing the Detector 5 Special Information of 60 mm Cartridge Flow Cell Application Information The geometrical volume of the 60 mm cell is 6 times larger than the 10 mm cell However the chromatographic relevant dispersion volume the square roots of variances accounting for cell specific geometrical volume shape and fluidic flow pattern have been determined as oV 4 uL and oV 1 uL in for the 10 mm cell Due to the larger dispersion volume the 60 mm cell is primarily designed for 4 6 mm column applications to achieve highest sensitivity with no additional peak broadening However if sensitivity is important the 60 mm cell will also be advantageous in case of smaller columns 3 mm 2 1 mm but depending on the chromatographic system and method a
150. pressure at which the system can operate continuously under normal 2 Maximum Incidential Pressure MIP The maximum pressure which the system can experience during a short time Agilent 1200 Infinity Series DAD User Manual 25 2 Site Requirements and Specifications Performance Specifications G4212B Table4 Performance Specifications G4212B Type Specification Comments Detection type Light source Wavelength range Short term noise ASTM Single and Multi Wavelength Drift Linear absorbance range Wavelength accuracy Wavelength bunching Slit width Diode width Signal data rate Spectra Data rate 1024 element photodiode array Deuterium lamp 190 640 nm lt 3 x 10 AU at 230 nm 4 nm with 10 mm Max Light cartridge cell Typically lt 0 6 x 10 AU cm at 230 nm 4 nm with 60 mm Max Light cartridge cell lt 0 5 x 10 AU hr at 230 nm gt 2 0 AU 5 at 265 nm 1nm 2 400 nm 4nm 0 5nm 80 Hz 80 Hz Equipped with RFID tag that holds lamp typical information see Specification Conditions below see Specification Conditions below see Specification Conditions below After recalibration with deuterium lines Programmable in steps of 1 nm Fixed slit 26 Agilent 1200 Infinity Series DAD User Manual Site Requirements and Specifications 2 Table4 Performance Specifications G4212B Type Specification Comments Flow cells Max Light Cartrid
151. r EMC regulations Agilent 1200 Infinity Series DAD User Manual 207 12 Hardware Information Electrical Connections Rear view of the module REMOTE ns 292 USB Prouct No G4212A ON Sedal No DEGurxtt0 BNI Agen Necbeategers AII Maran Made in Germany Figure 52 Rear View of Detector Electrical Connections and Label NOTE The CompactFlash Card Slot is not active yet It may be used for future enhancements 208 Agilent 1200 Infinity Series DAD User Manual Hardware Information 12 Information on Instrument Serial Number Serial Number Information 1200 Series and 1290 Infinity The serial number information on the instrument labels provide the following information CCYWWSSSSS CC YWW SSSSS Format country of manufacturing DE Germany e JP Japan e CN China year and week of last major manufacturing change e g 820 could be week 20 of 1998 or 2008 real serial number Serial Number Information 1260 Infinity The serial number information on the instrument labels provide the following information CCXZZ00000 CC ZZ 00000 Agilent 1200 Infinity Series DAD User Manual Format Country of manufacturing DE Germany JP Japan e CN China Alphabetic character A Z used by manufacturing Alpha numeric code 0 9 A Z w
152. r provides information on environmental requirements physical and performance specifications ee Agilent Technologies 19 2 Site Requirements and Specifications Site Requirements A suitable environment is important to ensure optimal performance of the module Power Consideration The module power supply has wide ranging capabilities and accepts any line voltage in the range mentioned in Table 2 on page 23 Consequently there is no voltage selector in the rear of the module There are also no externally accessible fuses because automatic electronic fuses are implemented in the power supply Module is partially energized when switched off as long as the power cord is plugged in Repair work at the module can lead to personal injuries e g shock hazard when the cover is opened and the module is connected to power gt Make sure that it is always possible to access the power plug gt Remove the power cable from the instrument before opening the cover gt Do not connect the power cable to the Instrument while the covers are removed Incorrect line voltage at the module Shock hazard or damage of your instrument can result if the devices are connected to line voltage higher than specified gt Connect your module to the specified line voltage CAUTION Inaccessible power plug In case of emergency it must be possible to disconnect the instrument from the power line at any time gt Make sure the power conn
153. re when the module shall obtain its parameters from a Bootp Server every time it is powered on the recommended initialization mode is Bootp 228 Agilent 1200 Infinity Series DAD User Manual LAN Configuration 13 Using Stored When initialization mode Using Stored is selected the parameters are taken from the non volatile memory of the module The TCP IP connection will be established using these parameters The parameters were configured previously by one of the described methods Non Volatile RAM Active Parameter Stored Parameter Figure 60 Using Stored Principle Using Default When Using Default is selected the factory default parameters are taken instead These parameters enable a TCP IP connection to the LAN interface without further configuration see Table 23 on page 229 Default Active Parameter Parameter Figure 61 Using Default Principle Using the default address in your local area network may result in network problems Take care and change it to a valid address immediately Table 23 Using Default Parameters IP address 192 168 254 11 Subnet Mask 255 255 255 0 Default Gateway not specified Agilent 1200 Infinity Series DAD User Manual 229 13 LAN Configuration 230 Since the default IP address is a so called local address it will not be routed by any network device Thus the PC and the module must reside in the same subnet The user may open a Telnet session using the defaul
154. resulting in significantly less baseline drift Figure 3 Max Light Cartridge Flow Cell For additional information on the Max Light Cartridge flow cell refer to Choosing a Flow Cell on page 80 and Inline Pressure Relief Valve Kit G4212 68001 on page 82 Agilent 1200 Infinity Series DAD User Manual 13 1 Introduction Optical System Slit Assembly Programmable Slit G4212A The micro slit system makes use of the mechanical properties of silicon combined with the precise structuring capabilities of bulk micro machining It combines the required optical functions slit and shutter in a simple and compact component The slit width is directly controlled by the micro processor of the instrument and can be set as method parameter Figure 4 Slit Assembly The slit width influences the spectral resolution and noise 14 Agilent 1200 Infinity Series DAD User Manual Introduction 1 Absorbance 230 240 250 260 270 280 nm 1nm AAAA 20m ghi y 4 nm a aa 8 nm Noise level Figure 5 Influence of slitwidth on resolution and noise level Fixed Slit G4212B The fixed slit combines the required optical functions slit and shutter in a simple and compact component The slit width is directly controlled by the micro processor of the instrument and is fixed to 4 nm In March 2011 the optical unit type of the G4212B DAD changed from Programmable Slit like in the G4212A fixed to 4 nm to a real Fixed S
155. ridge Cell a flow rate of 0 5 mL min with water is required 1 Run the Slit Test with the recommended user interface for further information see Online Help of user interface Test Name Slit Test Description The test performs a slit test Module G4212A PR00100015 Status Passed Start Time 719 2009 2 19 17 PM Stop Time 719 2009 2 19 56 PM ENCRECEECECENENENI Test Procedure m Result 7 Name Value WY 1 Check Prerequisites Cell Product Number 64212 60011 U 2 Ice spoed Ca or Test Cet Area Y 4 Evaluate Data Lamp Type Figure 43 Slit Test Results 151 Agilent 1200 Infinity Series DAD User Manual 8 Test Functions and Calibration Slit Test Figure 44 Slit Test Signals Test Failed Slit Test Evaluation Probable cause 1 Air bubble in Max Light Cartridge Cell 2 Old lamp 3 Defective slit assembly 4 Defective detector main board 5 Defective optical unit 152 Suggested actions Flush the flow cell or use the Max Light Cartridge Test Cell Run the Intensity Test Exchange the lamp if old or defective Please contact your Agilent service representative Please contact your Agilent service representative Please contact your Agilent service representative Agilent 1200 Infinity Series DAD User Manual Test Functions and Calibration 8 Slit Test G4212B There is no dedicated slit test for the G4212B DAD To verify the proper function perform the following tests e Intensity Te
156. rly Maintenance Feedback 221 This chapter describes the detector in more detail on hardware and electronics ott Agilent Technologies 203 12 Hardware Information Firmware Description The firmware of the instrument consists of two independent sections e anon instrument specific section called resident system e an instrument specific section called main system Resident System This resident section of the firmware is identical for all Agilent 1100 1200 1220 1260 1290 series modules Its properties are the complete communication capabilities CAN LAN and RS 232C memory management e ability to update the firmware of the main system Main System Its properties are the complete communication capabilities CAN LAN and RS 232C memory management e ability to update the firmware of the resident system In addition the main system comprises the instrument functions that are divided into common functions like e run synchronization through APG remote error handling diagnostic functions e or module specific functions like internal events such as lamp control filter movements raw data collection and conversion to absorbance 204 Agilent 1200 Infinity Series DAD User Manual Hardware Information 12 Firmware Updates Firmware updates can be done using your user interface PC and Firmware Update Tool with local files on the hard disk e Instant Pilot G4208A with files from a USB Flash Disk Agilent Lab Advisor
157. roduct or loss of important data Do not proceed beyond a CAUTION notice until the indicated condi tions are fully understood and met A WARNING notice denotes a hazard It calls attention to an operating procedure practice or the like that if not correctly performed or adhered to could result in personal injury or death Do not proceed beyond a WARNING notice until the indi cated conditions are fully under stood and met Agilent 1200 Infinity Series DAD User Manual In This Book In This Book This manual covers e the Agilent 1290 Infinity Diode Array Detector G4212A and e the Agilent 1260 Infinity Diode Array Detector G4212B Find information on other Agilent Diode Array Detectors in separate manuals 1 Introduction This chapter gives an introduction to the detector and an instrument overview 2 Site Requirements and Specifications This chapter provides information on environmental requirements physical and performance specifications 3 Installing the Module This chapter provides information on unpacking checking on completeness stack considerations and installation of the module 4 Using the Module This chapter provides information on how to set up the module for an analysis and explains the basic settings 5 Optimizing the Detector This chapter provides information on how to optimize the detector 6 Troubleshooting and Diagnostics Overview about the troubleshooting and diagnostic fea
158. rol ETC provides maximum baseline stability and practical sensitivity under fluctuating ambient temperature and humidity conditions Additional diagnostic signals for temperature and lamp voltage monitoring Easy exchange of flow cell by cartridge design For specifications see Performance Specifications G4212A on page 24 or Performance Specifications G4212B on page 26 Agilent 1200 Infinity Series DAD User Manual Introduction 1 Optical System Optical System The optical system of the detector is shown in Figure 1 on page 11 Figure 1 Optical System of the Detector 1 UV lamp 2 Lamp mirror 3 Flow cell 4 Fold mirror 5 Programmable G4212A or Fixed G4212B slit 6 Grating 7 Array The illumination source is a deuterium arc discharge lamp 1 for the ultraviolet UV wavelength range Its light is focused by a lamp mirror 2 onto the entrance of the Max light cartridge flow cell 3 with optofluidic waveguides The light leaves the Max light cartridge flow cell at the other side and is focused by the fold mirror 4 through the slit assembly 5 onto a holographic grating 6 light being dispersed onto the diode array 7 This allows simultaneous access to all wavelength information Agilent 1200 Infinity Series DAD User Manual 11 1 12 Introduction Lamp The light source for the UV wavelength range is a long life UV lamp with RFID tag As a result of plasma discharge in low pressure deut
159. roperties with regard to biocompatibility chemical resistance mechanical and thermal stability and is therefore the material of choice for biochemical instrumentation It is stable in the specified pH range and inert to many common solvents There is still a number of known incompatibilities with chemicals such as chloroform methylene chloride THF DMSO strong acids nitric acid gt 10 sulphuric acid gt 10 sulfonic acids trichloroacetic acid halogenes or aequous halogene solutions phenol and derivatives cresols salicylic acid etc When used above room temperature PEEK is sensitive to bases and various organic solvents which can cause it to swell As normal PEEK capillaries are very sensitive to high pressure especially under such conditions Agilent uses stainless steel cladded PEEK capillaries to keep the flow path free of steel and to ensure pressure stability to at least 600 bar If in doubt consult the available literature about the chemical compatibility of PEEK Titanium Titanium is highly resistant to oxidizing acids for example nitric perchloric and hypochlorous acid over a wide range of concentrations and temperatures This is due to a thin oxide layer on the surface which is stabilized by oxidizing compounds Reducing acids for example hydrochloric sulfuric and phosphoric acid can cause slight corrosion which increases with acid concentration and temperature For example the corrosion rate with 3 HCl
160. rs continually monitor the system configuration If one or more of the modules is no longer recognized as being connected to the system the error message is generated Probable cause Suggested actions 1 CAN cable disconnected Ensure all the CAN cables are connected correctly Ensure all CAN cables are installed correctly 2 Defective CAN cable Exchange the CAN cable 3 Defective main board in another module Switch off the system Restart the system and determine which module or modules are not recognized by the system Leak Sensor Short Error ID 0082 The leak sensor in the module has failed short circuit The current through the leak sensor is dependent on temperature A leak is detected when solvent cools the leak sensor causing the leak sensor current to change within defined limits If the current increases above the upper limit the error message is generated Probable cause Suggested actions 1 Defective leak sensor Please contact your Agilent service representative 2 Leak sensor incorrectly routed being e Please contact your Agilent service pinched by a metal component representative Correct the routing of the cable If cable defective exchange the leak sensor Agilent 1200 Infinity Series DAD User Manual 121 7 Error Information Leak Sensor Open Error ID 0083 The leak sensor in the module has failed open circuit The current through the leak sensor is dependent on temperature A leak is d
161. s 25 27 flow connections 44 44 frequency range 23 G general error messages 119 general method settings using 58 GLP features 25 27 grating 16 GUI detector 53 H humidity 23 initialization mode selection 227 inline pressure relief valve kit 82 190 installation accessory kit 31 bench space 22 delivery checklist 31 detector 41 environment 22 flow connections 44 44 ofthe detector 41 power considerations 20 Instant Pilot using 69 instrument configuration using 67 instrument curves using 66 instrument layout 220 intensity test 140 interfaces 210 internet 270 L lamp uv 12 LAN configuration ChemStation 254 PC setup 251 LAN automatic configuration with Bootp 235 Agilent 1200 Infinity Series DAD User Manual Bootp amp Store 228 Bootp 227 cable 201 configuration switche 226 first steps 224 initialization mode selection 227 link configuration selection 234 manual configuration with telnet 246 manual configuration 245 PC and Agilent ChemStation Setup 251 TCP IP parameter configuration 225 using default 229 using stored 229 what you have to do first 224 eak sensor open 122 eak sensor short 121 eak 124 ine frequency 23 ine voltage 23 inearrange 24 26 inearity specifications 28 ink configuration selection 234 ocal configuration 251 ost CAN partner 121 M MAC address determine 240 MAC address 224 maintenance definition of 166 drying the leak sensor 180 feedback 221 lamp and flow c
162. s and Calibrations Diagnostic Signals The module has several signals internal temperatures voltages and currents of lamps that can be used for diagnosing baseline problems These can be added like normal signals in the Agilent ChemStation software For details see Instrument Curves on page 66 110 Agilent 1200 Infinity Series DAD User Manual Troubleshooting and Diagnostics 6 Status Indicators Status Indicators Two status indicators are located on the front of the module The lower left indicates the power supply status the upper right indicates the module status Status indicator I I Tr st i _ ower supply indicator Figure 32 Location of Status Indicators Power Supply Indicator The power supply indicator is integrated into the main power switch When the indicator is illuminated green the power is ON Agilent 1200 Infinity Series DAD User Manual 111 6 Troubleshooting and Diagnostics Module Status Indicator The module status indicator indicates one of six possible module conditions 112 When the status indicator is OFF and power switch light is on the module is ina prerun condition and is ready to begin an analysis A green status indicator indicates the module is performing an analysis run mode A yellow indicator indicates a not ready condition The module is ina not ready state when it is waiting for a specific condition to be reached or
163. s are in compliance with safety or EMC regulations Test and Measurement If test and measurement equipment is operated with unscreened cables or used for measurements on open set ups the user has to assure that under operating conditions the radio interference limits are still met within the premises Agilent 1200 Infinity Series DAD User Manual 267 14 Appendix Sound Emission Manufacturer s Declaration This statement is provided to comply with the requirements of the German Sound Emission Directive of 18 January 1991 This product has a sound pressure emission at the operator position lt 70 dB e Sound Pressure Lp lt 70 dB A At Operator Position e Normal Operation e According to ISO 7779 1988 EN 27779 1991 Type Test 268 Agilent 1200 Infinity Series DAD User Manual Appendix 14 Solvent Information Flow Cell To protect optimal functionality of your flow cell The recommended pH range of the cell is 1 0 12 5 solvent dependent If the flow cell is transported while temperatures are below 5 degree C it must be assured that the cell is filled with alcohol e Aqueous solvents in the flow cell can built up algae Therefore do not leave aqueous solvents sitting in the flow cell Add a small of organic solvents e g acetonitrile or methanol 5 Use of Solvents Observe the following recommendations on the use of solvents e Brown glass ware can avoid growth of algae Avoid the use of the following ste
164. service make the changes and then restart the service Stopping the Agilent BootP Service 1 From the Windows control panel select Administrative Tools gt Services The Services screen appears RE e aton yew tep s B DPRI gt r gt s n Select an tem to view ts description BoArtomatic Updates Rated Beadgound inteky DQ chlor coms Evert System S s Rated Rated f Provides th Rated Rated Manages Started Boderrbeted ink Tra Martorsh Rated Byvetrdeted Transac Coordinate a P aesan a j Figure 67 Windows Services screen 2 Right click Agilent BootP Service 3 Select Stop 4 Close the Services and Administrative Tools screen Editing the IP address and other parameters in EditBootPSettings 1 Select Start gt All Programs gt Agilent BootP Service and select Edit BootP Settings The BootP Settings screen appears 2 When the BootP Settings screen is first opened it shows the default settings from installation Agilent 1200 Infinity Series DAD User Manual 243 13 LAN Configuration 3 4 5 6 Press Edit BootP Addresses to edit the Tab File xj 006000111999 Agler l C1 1011 10 Ager LCI igh 2552552550 00 00 005000222863 Agler C2 101 1 1 102 AgkertlC2ket 25525250 0000 Figure 68 Edit BootP Adresses screen In the Edit BootP Addresses screen press Add to create a new entry or select an existing line from the table and press Modify or Delete to change the
165. software B 01 03 and above The file naming conventions are PPPP_RVVV_XXX dlb where PPPP is the product number for example 1315AB for the G1315A B DAD R the firmware revision for example A for G1315B or B for the G1315C DAD VVV is the revision number for example 102 is revision 1 02 XXX is the build number of the firmware For instructions on firmware updates refer to section Replacing Firmware in chapter Maintenance or use the documentation provided with the Firmware Update Tools Update of main system can be done in the resident system only Update of the resident system can be done in the main system only Main and resident firmware must be from the same set Main FW update Resident System Main System Resident FW Update Figure 51 Firmware Update Mechanism Agilent 1200 Infinity Series DAD User Manual 205 12 Hardware Information 206 Some modules are limited in downgrading due to their main board version or their initial firmware revision For example a G1315C DAD SL cannot be downgraded below firmware revision B 01 02 or to a A xx xx Some modules can be re branded e g G1314C to G1314B to allow operation in specific control software environments In this case the feature set of the target type are use and the feature set of the original are lost After re branding e g from G1314B to G1314C the original feature set is available again All these specific informations are described in the document
166. st tests the normal position Dark Current Test tests the dark position Agilent 1200 Infinity Series DAD User Manual 153 8 Test Functions and Calibration Wavelength Verification Test The detector uses the alpha 656 1 nm and beta 486 nm emission lines of the UV lamp for wavelength calibration The sharp emission lines enable accurate calibration When verification is started the 1 nm slit is moved into the light path automatically The test is run with the Max Light Cartridge Cell or with Max Light Cartridge Test Cell installed If the test is performed with the Max Light Cartridge Test Cell the test results are not influenced by solvent or pump effects When The detector is calibrated at the factory and under normal operating conditions should not require recalibration However it is advisable to recalibrate after repair of components in the optical unit after exchange of the optical unit or main board after replacing the Max Light Cartridge Cell or UV lamp after significant environmental condition changes temperature humidity ata regular interval at least once per year for example prior to an Operational Qualification Performance Verification procedure and when chromatographic results indicate the detector may require recalibration Parts required Description 1 Max Light Cartridge Test Cell or 1 Max Light Cartridge Cell Preparations Lamp must be on for at least 10 minutes When usin
167. stalled on a server or PC on the LAN to provide central administration of IP addresses for Agilent instruments on a LAN The BootP service must be running TCP IP network protocol and cannot run a DHCP server Agilent 1200 Infinity Series DAD User Manual 235 13 LAN Configuration 236 How BootP Service Works When an instrument is powered on an LAN Interface in the instrument broadcasts a request for an IP address or host name and provides its hardware MAC address as an identifier The Agilent BootP Service answers this request and passes a previously defined IP address and host name associated with the hardware MAC address to the requesting instrument The instrument receives its IP address and host name and maintains the IP address as long as it is powered on Powering down the instrument causes it to lose its IP address so the Agilent BootP Service must be running every time the instrument powers up If the Agilent BootP Service runs in the background the instrument will receive its IP address on power up The Agilent LAN Interface can be set to store the IP address and will not lose the IP address if power cycled Situation Cannot Establish LAN Communication If a LAN communication with BootP service cannot be established check the following on the PC e Is the BootP service started During installation of BootP the service is not started automatically Does the Firewall block the BootP service Add the BootP service as an
168. t 0 means upper power limit hit excessive ambient temperature drop 1 means lower power limit hit excessive ambient temperature increase Probable cause Suggested actions 1 Excessive ambient temperature change Wait until temperature control equilibrates Agilent 1200 Infinity Series DAD User Manual 131 7 Error Information Detector Error Messages 132 Agilent 1200 Infinity Series DAD User Manual Agilent 1200 Infinity Series DAD User Manual 8 Test Functions and Calibration Introduction 134 Use of Max Light Cartridge Test Cell 135 Conditions of Detector 136 Failing a Test 137 Self Test 138 Intensity Test 140 Test Failed 142 Cell Test 143 Test Failed low ratio value 144 Quick Noise Test 145 Test Failed 147 ASTM Drift and Noise Test 148 Test Failed 150 Slit Test 151 Slit Test G4212A 151 Test Failed 152 Slit Test G4212B 153 Wavelength Verification Test 154 Wavelength Calibration 156 Wavelength Recalibration Fails 158 D A Converter DAC Test 159 Test Evaluation 160 Dark Current Test 161 Test Failed 162 This chapter describes the tests for the module oe Agilent Technologies 133 8 Test Functions and Calibration Introduction All tests are described based on the Agilent Lab Advisor Software B 01 03 Other user interfaces may not provide any test or just a few Table 10 Interfaces and available test functions Interface Comment Available Function Agilent Instrument Utilities Maintenance
169. t To determine the volume of your peaks take the peak width as reported in the integration results multiply it by the flow rate and divide it by 3 This may result in problems when the used peak width is set to large and all peaks are filtered accordingly Traditionally LC analysis with UV detectors is based on comparing measurements with internal or external standards To check photometric accuracy of the Agilent detector it is necessary to have more precise information on path lengths of the detector flow cells Part Number Path Length Cell Volume C G4212 60008 G5615 60018 1 0 cm 1 0 pL G4212 60007 G5615 60017 6 0 cm 4 0 uL 86 Agilent 1200 Infinity Series DAD User Manual Optimizing the Detector 5 Peak width response time Response time describes how fast the detector signal follows a sudden change of absorbance in the flow cell The detector uses digital filters to adapt response time to the width of the peaks in your chromatogram These filters do not affect peak area nor peak symmetry When set correctly such filters reduce baseline noise significantly Figure 19 on page 87 but reduce peak height only slightly In addition these filters reduce the data rate to allow optimum integration and display of your peaks and to minimize disk space required to store chromatograms and spectra DAOI A Sigr240 100 Rem300 100 0 42 12AINOL_PW_1E0H D DADI A Sigr280 100 Rete300 100 0 42 2ANNOI_PW_2EM D mau Response time
170. t IP address and change the parameters stored in the non volatile memory of the module He may then close the session select the initialization mode Using Stored power on again and establish the TCP IP connection using the new parameters When the module is wired to the PC directly e g using a cross over cable or a local hub separated from the local area network the user may simply keep the default parameters to establish the TCP IP connection In the Using Default mode the parameters stored in the memory of the module are not cleared automatically If not changed by the user they are still available when switching back to the mode Using Stored Agilent 1200 Infinity Series DAD User Manual LAN Configuration 13 Dynamic Host Configuration Protocol DHCP General Information DHCP The Dynamic Host Configuration Protocol DHCP is an auto configuration protocol used on IP networks The DHCP functionality is available on all Agilent HPLC modules with on board LAN Interface or LAN Interface Card and B firmware B 06 40 or above When the initialization mode DHCP is selected the card tries to download the parameters from a DHCP Server The parameters obtained become the active parameters immediately They are not stored to the non volatile memory of the card Besides requesting the network parameters the card also submits its hostname to the DHCP Server The hostname equals the MAC address of the card e g 0030d
171. te the two screws and fix the lamp 9 Fit the lamp wires in the lamp house cover so that they are not scratched by the cover Agilent 1200 Infinity Series DAD User Manual 171 9 Maintenance Replacing the Deuterium Lamp 11 Close the front cover 12 Perform a Wavelength Re calibration after lamp warm up 172 Agilent 1200 Infinity Series DAD User Manual Maintenance 9 Replacing the Max Light Cartridge Cell BIO When Tools required Parts required Preparations CAUTION For bio inert modules use bio inert parts only If leaking or if intensity drops due to contaminated flow cell Description Wrench hexagonal p n Description 1 G4212 60008 Max Light Cartridge Cell 10 mm V o 1 0 uL 1 G4212 60007 Max Light Cartridge Cell 60 mm V o 4 0 uL 1 G4212 60011 Max Light Cartridge Test Cell 1 G5615 60018 Max Light Cartridge Cell Bio inert 10 mm V o 1 0 uL includes Peek Capillary 1 5 mi d 0 18 mm 0890 1763 and PEEK Fittings 10 PK 5063 6591 1 G5615 60017 Max Light Cartridge Cell Bio inert 60 mm V o 4 0 uL includes Peek Capillary 1 5 mi d 0 18 mm 0890 1763 and PEEK Fittings 10 PK 5063 6591 1 G4212 60032 HDR Max Light Cartridge Cell 3 7 mm V o 0 4 pL 1 G4212 60038 ULD Max Light Cartridge Cell 10 mm V o 0 6 pL Turn the pump off Sample degradation and contamination of the instrument Metal parts in the flow path can interact with th
172. the Detector with Agilent ChemStation The setup of the detector is shown with the Agilent ChemStation B 04 02 based on the 1290 Infinity DAD G4212A Depending on the controller e g Agilent Instant Pilot EZChrom Elite MassHunter the screens look different For the Instant Pilot refer to Main Screens of the Detector with Agilent Instant Pilot G4208A on page 69 This section describes the detector settings only For information of the Agilent ChemStation or other Agilent 1260 Infinity 1290 Infinity Series modules refer to the corresponding documentation or system manual After successful load of the ChemStation you should see the module as an active item in the graphical user interface GUI Agilent 1200 Infinity Series DAD User Manual 51 4 Using the Module Setting up the Detector with Agilent ChemStation nil D NotReady a Instrument 192 168 254 11 0 00 0 00 Not Ready Figure 16 ChemStation Method amp Run 52 Agilent 1200 Infinity Series DAD User Manual The Detector GUI 1290 Infinity DAD Not Ready Setting up the Detector with Agilent ChemSte WL BWRefWL RefBW mAU 254 0 210 0 214 0 230 0 260 0 Agilent 1200 Infinity Series DAD User Manual 4 4 4 4 4 360 0 360 0 360 0 360 0 360 0 100 100 100 100 100 Using the Module 4 Within the detector GUI there are active areas If you move the mouse cursor across the icons the cursor will
173. tion 51 The Detector GUI 53 Control Settings 56 Method Parameter Settings 57 General Method Settings 58 Advanced Method Parameter Settings 60 Spectrum Settings 61 Other Advanced Method Parameter Settings 63 Time Table Settings 64 Instrument Curves 66 Instrument Configuration 67 Main Screens of the Detector with Agilent Instant Pilot G4208A 69 Solvent Information 73 This chapter provides information on how to set up the module for an analysis and explains the basic settings pe Agilent Technologies 49 4 Using the Module Preparing the Detector 50 For best performance of the detector Let the lamp warm up and stabilize for at least one hour initial turn on of the module requires a longer time depending on the environment and the application needs refer to Specification Conditions on page 28 For high sensitivity measurements a stable environment is required refer to Environment on page 22 Prevent drafts from air condition systems Setting an appropriate reference wavelength could improve the baseline behavior Alternatively use the 1 6 uL heat exchanger from the G1316C TCC Do not work with removed front panels When the front panel of the G1316C TCC typically located below the detector is removed while the TCC is set to high temperatures the up streaming air could influence the stability of the detector baseline Agilent 1200 Infinity Series DAD User Manual Using the Module 4 Setting up
174. to Cable Overview on page 192 Agilent 1200 Infinity Series DAD User Manual Parts and Materials for Maintenance 10 Overview of Maintenance Parts Agilent 1200 Infinity Series DAD User Manual 189 10 Parts and Materials for Maintenance Kits Accessory Kit Accessory kit G4212 68755 contains some specific accessories and materials needed for the installation of the detector p n 5062 2462 5063 6527 5042 9967 0100 1516 5067 4660 5181 1516 Description PTFE Tubing flexible i d 0 8 mm o d 1 6 mm 2 m re order 5 m flow cell to waste Tubing assembly i d 6 mm o d 9 mm 1 2 m to waste Tubing clip set of 5 clips Fitting male PEEK 2 pk Inlet Capillary SST 0 12 mm I D 220 mm long CAN cable Agilent module to module 0 5 m Inline Pressure Relief Valve Kit G4212 68001 To protect the flow cell against overpressure please refer to Inline Pressure Relief Valve Kit G4212 68001 on page 82 190 Agilent 1200 Infinity Series DAD User Manual Agilent 1200 Infinity Series DAD User Manual 11 Identifying Cables Cable Overview 192 Analog Cables 194 Remote Cables 196 BCD Cables 199 CAN LAN Cables 201 RS 232 Cables 202 This chapter provides information on cables used with the Agilent 1260 Infinity 1290 Infinity LC modules Ee Agilent Technologies 191 11 Identifying Cables Cable Overview 192 Never use cables other than the ones supplied by Agilent Technologies to ens
175. ttings Ensure all fittings are tight 2 Broken capillary Exchange defective capillaries Open Cover Error ID 0205 The top foam has been removed Probable cause Suggested actions 1 Foam not activating the sensor Please contact your Agilent service representative 2 Defective sensor or main board Please contact your Agilent service representative 124 Agilent 1200 Infinity Series DAD User Manual Error Information 7 Cover Violation Error ID 7461 The top foam has been removed The sensor on the main board detects when the top foam is in place If the foam is removed while the lamps are on or if an attempt is made to switch on for example the lamps with the foam removed the lamps are switched off and the error message is generated Probable cause Suggested actions 1 The top foam was removed during Please contact your Agilent service operation representative 2 Foam not activating the sensor Please contact your Agilent service representative Agilent 1200 Infinity Series DAD User Manual 125 7 Error Information Detector Error Messages These errors are detector specific Diode Current Leakage Error ID 1041 When the detector is switched on the processor checks the leakage current of each of the optical diodes If the leakage current exceeds the upper limit the error message is generated Probable cause Suggested actions 1 Defective PDA optical unit Please contact your Agilent service representative
176. tures 7 Error Information This chapter describes the meaning of error messages and provides information on probable causes and suggested actions how to recover from error conditions Agilent 1200 Infinity Series DAD User Manual 3 In This Book 8 Test Functions and Calibration This chapter describes the tests for the module 9 Maintenance This chapter describes the maintenance of the module 10 Parts and Materials for Maintenance This chapter provides information on parts for maintenance 11 Identifying Cables This chapter provides information on cables used with the Agilent 1260 Infinity 1290 Infinity LC modules 12 Hardware Information This chapter describes the detector in more detail on hardware and electronics 13 LAN Configuration This chapter provides information on connecting the module to the Agilent ChemStation PC 14 Appendix This chapter provides addition information on safety legal and web Agilent 1200 Infinity Series DAD User Manual Contents Contents 1 Introduction 9 Overview of the Module 10 Optical System 11 Bio inert Materials 17 2 Site Requirements and Specifications 19 Site Requirements 20 Physical Specifications 23 Performance Specifications 24 3 Installing the Module 29 Unpacking the Module 30 Optimizing the Stack Configuration 32 Installing the Detector 41 Flow Connections to the Detector 44 Initial Recalibration 48 4 Usingthe Module 49 Preparing the D
177. ule Description 1 Firmware tools and documentation from Agilent web site Read update documentation provided with the Firmware Update Tool To upgrade downgrade the module s firmware carry out the following steps 1 Download the required module firmware the latest LAN RS 232 FW Update Tool and the documentation from the Agilent web http www chem agilent com _layouts agilent downloadFirmware aspx whid 69761 2 For loading the firmware into the module follow the instructions in the documentation Agilent 1200 Infinity Series DAD User Manual 183 9 Maintenance Table 13 Module Specific Information G4212A G4212B G4212A 1290 DAD G4212B 1260 DAD Initial firmware main and resident Compatibility with 1100 1200 1260 1290 series modules Compatibility with VSA Optical B 06 23 B 06 30 When using the G4212A in a system all other When using the G4212B in a system all other modules must have firmware revision A 06 1x or modules must have firmware revision A 06 3x or B 06 1x and above main and resident B 06 3x and above main and resident Otherwise the communication will not work Otherwise the communication will not work Introduced 07 2012 Firmware B 06 51 B 06 43 or B 06 26 or later depends on the used firmware set Earlier revisions are not compatible with the VSA Optical These revisions are the required versions for the new VSA Optical Unit and Main Boards Conversion to N A N A emulation
178. um settings 61 time table settings 64 V voltage range 23 Ww warm up of detector 107 warnings and cautions 164 waste electrical and electronic equipment 266 wavelength and bandwidth optimization 102 wavelength calibration 156 wavelength recalibration lost 129 wavelength verification test 154 wavelength accuracy 24 26 Agilent 1200 Infinity Series DAD User Manual Index 275 www agilent com In This Book This manual contains technical reference information about the Agilent 1290 Infinity Diode Array Detector G4212A and the Agilent 1260 Infinity Diode Array Detector G4212B introduction and specifications e installation e using and optimizing troubleshooting and diagnose e maintenance e parts identification safety and related information Agilent Technologies 2010 2011 2012 2013 Printed in Germany 02 2013 G4212 90013 EE Agilent Technologies
179. ure proper functionality and compliance with safety or EMC regulations Analog cables p n 35900 60750 35900 60750 01046 60105 Remote cables p n 03394 60600 03396 61010 5061 3378 01046 60201 BCD cables p n 03396 60560 G1351 81600 Description Agilent module to 3394 6 integrators Agilent 35900A A D converter Analog cable BNC to general purpose spade lugs Description Agilent module to 3396A Series integrators 3396 Series II 3395A integrator see details in section Remote Cables on page 196 Agilent module to 3396 Series III 3395B integrators Remote Cable Agilent module to general purpose Description Agilent module to 3396 integrators Agilent module to general purpose Agilent 1200 Infinity Series DAD User Manual Identifying Cables 11 CAN cables p n Description 5181 1516 CAN cable Agilent module to module 0 5 m 5181 1519 CAN cable Agilent module to module 1 m LAN cables p n Description 5023 0203 Cross over network cable shielded 3 m for point to point connection 5023 0202 Twisted pair network cable shielded 7 m for point to point connection RS 232 cables p n Description G1530 60600 RS 232 cable 2 m RS232 61601 RS 232 cable 2 5 m Instrument to PC 9 to 9 pin female This cable has special pin out and is not compatible with connecting printers and plotters It s also called Null Modem Cable with full handshaking where the wiring is made between pins 1 1 2
180. use and can be assigned a maximum limit which provides visual feedback in the user interface when the limit is exceeded Some counters can be reset to zero after the required maintenance procedure Using the EMF Counters The user settable EMF limits for the EMF Counters enable the early maintenance feedback to be adapted to specific user requirements The useful maintenance cycle is dependent on the requirements for use Therefore the definition of the maximum limits need to be determined based on the specific operating conditions of the instrument Setting the EMF Limits The setting of the EMF limits must be optimized over one or two maintenance cycles Initially the default EMF limits should be set When instrument performance indicates maintenance is necessary take note of the values displayed by the EMF counters Enter these values or values slightly less than the displayed values as EMF limits and then reset the EMF counters to zero The next time the EMF counters exceed the new EMF limits the EMF flag will be displayed providing a reminder that maintenance needs to be scheduled Agilent 1200 Infinity Series DAD User Manual 221 12 Hardware Information Early Maintenance Feedback 222 Agilent 1200 Infinity Series DAD User Manual Agilent 1200 Infinity Series DAD User Manual 13 LAN Configuration What You Have to Do First 224 TCP IP parameter configuration 225 Configuration Switch 226 Initialization mode selection 227
181. used previously in a G4212B K4212B DAD The detector will boot as a G4212B DAD no error The DAD has the functionality of the G4212B K4212B DAD Enter the correct serial number as written on the module label The DAD can be reverted back to G4212A with Lab Advisor Board Check and Change Reboot the DAD if not done automatically Normal Routine The normal routine is as follows The main board has no type information During boot of the DAD it retrieves the module type G4212A or G4212B DAD from the installed optical unit and keeps it Via Board Check and Change add the serial number Reboot the DAD if not done automatically Agilent 1200 Infinity Series DAD User Manual Agilent 1200 Infinity Series DAD User Manual 00 ee 7 70 Error Information e e i e i What Are Error Messages 118 General Error Messages 119 Timeout 119 Shutdown 120 Remote Timeout 120 Lost CAN Partner 121 Leak Sensor Short 121 Leak Sensor Open 122 Compensation Sensor Open 122 Compensation Sensor Short 123 Fan Failed 123 Leak 124 Open Cover 124 Cover Violation 125 Detector Error Messages 126 Diode Current Leakage 126 UV Lamp Current 126 UV Lamp Voltage 127 UV Ignition Failed 127 UV Heater Current 128 Calibration Values Invalid 128 Wavelength Recalibration Lost 129 Illegal Temperature Value from Sensor on Main Board 129 Illegal Temperature Value from Sensor at Fan Assembly 130 Heater at fan assembly failed 130 Heater Power At Li
182. x Light Cartridge Cell 178 Agilent 1200 Infinity Series DAD User Manual Contents Storage of Max Light Cartridge Cell 179 Drying the Leak Sensor 180 Replacing Leak Handling System Parts 181 Replacing Module Firmware 183 Information from Module s Assemblies 185 10 Parts and Materials for Maintenance 187 Overview of Maintenance Parts 188 Kits 190 11 Identifying Cables 191 Cable Overview 192 Analog Cables 194 Remote Cables 196 BCD Cables 199 CAN LAN Cables 201 RS 232 Cables 202 12 Hardware Information 203 Firmware Description 204 Electrical Connections 207 Interfaces 210 Setting the 8 bit Configuration Switch 217 Instrument Layout 220 Early Maintenance Feedback 221 13 LAN Configuration 223 What You Have to Do First 224 TCP IP parameter configuration 225 Configuration Switch 226 Initialization mode selection 227 Dynamic Host Configuration Protocol DHCP 231 Link configuration selection 234 Automatic Configuration with BootP 235 Manual Configuration 245 PC and Agilent ChemStation Setup 251 Agilent 1200 Infinity Series DAD User Manual Contents 14 Appendix 261 Safety 262 The Waste Electrical and Electronic Equipment Directive 266 Radio Interference 267 Sound Emission 268 Solvent Information 269 Agilent Technologies on Internet 270 8 Agilent 1200 Infinity Series DAD User Manual Agilent 1200 Infinity Series DAD User Manual 1 Introduction Overview of the Module 10 Optical System 11
183. ximum Operating Pressure MOP The maximum pressure at which the system can operate continuously under normal conditions 2 Maximum Incidential Pressure MIP The maximum pressure which the system can experience during a short time Normal Applications The Max Light Cartridge Cell 10 mm V o 1 0 uL G4212 60008 covers a wide range of applications all column diameter down to at least 2 1 mm ID or even less applications with peak dispersion Peakwidth x flow down to 2 uL example pw 0 04 min at flow 0 1 mL min gives peak dispersion of 0 04 min x 0 1 mL min 0 004 mL 4 uL High Sensitiviy If higher sensitivity is necessary the Max Light Cartridge Cell 60 mm V o 4 0 uL G4212 60007 can be used This cell enhances the detector by lowering the limit of detection LOD by a factor of about 3 depending on the application Agilent 1200 Infinity Series DAD User Manual Optimizing the Detector 5 Ultra Low Dispersion The Max Light Cartridge ULD cell can be used with the G4212A DAD and G4212B DAD The cell is a requirement for the Ultra Low Dispersion Kit solution which currently exists as 1290 Infinity Ultra Low Dispersion Kit 5067 5189 The cell should be part of the ultra low dispersion solution High Dynamic Range The Max Light Cartridge HDR cell can be used with the G4212A DAD and G4212B DAD The cell is required as a part of the High Dynamic Range HDR solution which will be introduced March April
184. y or initialized with known default values The initialization mode is selected by the configuration switch see Table 22 on page 227 Agilent 1200 Infinity Series DAD User Manual 225 13 LAN Configuration Configuration Switch 226 The configuration switch can be accessed at the rear of the module Figure 57 Location of Configuration Switch The module is shipped with all switches set to OFF as shown above To perform any LAN configuration SW1 and SW2 must be set to OFF Table 21 Factory Default Settings Initialization Init Mode Bootp all switches down For details see Initialization mode selection on page 227 Link Configuration speed and duplex mode determined by auto negotiation for details see Link configuration selection on page 234 Agilent 1200 Infinity Series DAD User Manual LAN Configuration 13 Initialization mode selection Table 22 The following initialization init modes are selectable Initialization Mode Switches SW6 SW7 SW8 Init Mode OFF OFF OFF Bootp OFF OFF ON Bootp amp Store OFF ON OFF Using Stored OFF ON ON Using Default ON OFF OFF DHCP 1 Requires firmware B 06 40 or above Modules without LAN on board see G1369C LAN Interface Card Bootp When the initialization mode Bootp is selected the module tries to download the parameters from a Bootp Server The parameters obtained become the active p
185. y select the module manually from the list We use the Auto Configuration mode Click on Yes yen TIERT Qu Qe G sewo Puo GG normo Off cotmecone Agilent 1100 1200 1290 Series LE Auto Configurati 3 14 Click on OK The selected module is shown now in the right window with serial number In addition all other modules connected via CAN to the detector are shown as well Agilent 1200 Infinity Series DAD User Manual 257 13 LAN Configuration PC and Agilent ChemStation Setup 15 Click on OK to continue the ChemStation loading instrument Configuration Agilent 1100712007 1290 Series LC Agilent 1100 1200 1290 Series LC Qs SH 040 1642124 PROMIO0N5 Dros i wre GG oaro of cstnecone 16 You may see the details of the module by selecting the module and clicking on Configure 1100 1200 1290 DAD Configuration Instrument 1 Under Connection Settings you may change the IP Hostname of the module may require a re start of the ChemStation After successful load of the ChemStation you should see the module s as active item in the graphical user interface GUI 258 Agilent 1200 Infinity Series DAD User Manual LAN Configuration 13 PC and Agilent ChemStation Setup Instrument 0 00 0 00 Not Ready Figure 77 Screen After Successful Load of ChemStation Agilent 1200 Infinity Series DAD User Manual 259 13 LAN Configuration PC and Agilent ChemStat

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