SHUTTLE BOX LEARNED HELPLESSNESS
Contents
1. 8 8 3 Sl N Initiate Trials 2 Start CS IF L 1 LeftTrial Next LeftTrial lt S5 Next IF L 2 GRightTrial Error RightTrial gt S10 Error lt SX S5 Trial Sequence Starting from Left Chamber CS Period Activate Shock Left Side A CS Time T ON LeftGrid IF A OverLapTag gt 1 OverLap NoOverLap QOverLap ON ShockOperate S87 NoOverLap ON ShockOperate Z End CS lt S7 Z EnterRight ADD D I 7 B TotalAvoids ADD D I 3 B TotalCrossings Z Avoid Z End CS lt 1 Sys UCS Period Left Chamber A UCS Time T OFF ShockOperate LeftGrid ADD D I 4 B TotalFailures Z Start ITI Z End CS lt 1 Z EnterRight ADD D I 9 B TotalEscapes ADD D I 3 B TotalCrossings Z Escape Z End CS lt S8 S8 Shock OFF Delay to Force a Complete Crossing l 5 i OFF ShockOperate 28150910 lt SI crc See S10 S13 are identical in function to S5 S8 Above 2 2 2 2 22 22222222 222222002 S10 Trial Sequence Starting from Right Chamber CS Period Activate Shock Right Side A CS Time 4T ON RightGrid IF A OverLapTag gt 1 OverLap NoOverLap QOverLap ON ShockOperate 12 NoOverLap ON ShockOperate Z End CS lt S12 Z2 EnterLeft ADD D I 7 B T2. CK OK eK CK KO ok Kok OG Ge 8 8 2 Set Location Flag L and issue Location Z Pulse Sl R LeftIR 1 amp R LeftIR 2 R LeftIR 3 R LeftIR_4 SET L 1 gt 2 R RightIR 1 amp R RightIR 2 R RightIR_3 R RightIR_4 SET L 2 lt S4 S2 Animal on Left Grid wait for Beam break on Right Side R RightIR 1 amp R RightIR 2 R RightIR_3 R RightIR 4 gt S3 Z EndSession gt S6 S3 Check to see if any Left Side Beams are being broken if not then count it 88 a crossing to the Right Side R LeftIR 4 R LeftIR 3 amp R LeftIR 2 R LeftIR 1 2 0 02 SET L 2 Z EnterRight lt 4 Z EndSession 6 S4 Animal on Right Grid wait for Beam break on Left Side R LeftIR 1 amp R LeftIR 2 R LeftIR 3 R LeftIR_4 lt 5 Z EndSession 6 85 Check to see if any Right Side Beams are being broken if not then count it as a crossing to the Left Side R RightIR 1 R RightIR_2 R RightIR_3 R RightIR 4 gt 4 0 02 SET L 1 Z EnterLeft gt S2 Z EndSession S6 S6 Holding State at End of Session Pu SX 20 DESCRIPTION OF STATE SET 3 State Set 3 controls the trial sequence for when the subject starts the trial on the left S1 S5 S8 and right S10 S13 side of the chamber NCOKCKCk KK kk KK KK kk KKK kk KK kk ck Ok ck k ck ck N TRIAL SEQUENCE AND DATA COLLECTION
3. Movement following a Beam 2 Break Z EndSession 1 2 ADD B LeftMovement D I 1 lt 4 2 ADD B LeftMovement D I 1 lt S6 Z One ADD B LeftMovement 2 1 1 lt S7 S6 Movement following a Beam 3 Break Z EndSession gt 1 Z Four ADD B LeftMovement D I 1 gt S4 Z Three ADD B LeftMovement D I 1 lt S5 Z One ADD B LeftMovement D I 1 gt S7 S7 Movement following a Beam 4 Break tz EndSession 1 Z Four ADD B LeftMovement D I 1 lt S4 Z Three ADD B LeftMovement D I 1 lt S5 Z Two ADD B LeftMovement D I 1 gt S6 NCOKCKCk KK kk KR KK kkk kkk kk k k kk k KK kk ck KK KK KK k ck k k k k k k RIGHT MOVEMENT ACTIVITY 8 9 11 S1 START lt S2 82 N Wait for Start of First Trial Z Start A S3 3 Wait for first Beam Break Z Five gt 4 Z Six 85 Z Seven lt S6 Z Eight 7 S4 Movement following a Beam 5 Break Z EndSession gI ADD B RightMovement D I 2 lt 5 Z Seven ADD B RightMovement D I 2 lt S6 Z Eight ADD B RightMovement D I 2 lt S7 85 Movement following a Beam 6 Break Z EndSession SI Z Five ADD B RightMovement 2 1 2 lt
4. lt S2 82 FfR LeftIR 1 Z Four gt S3 S3 PRR LeftIR 1 gt SX 0 01 2 ck k ck k k ck k k k k ck k k k k kk N BEAM 5 BREAK 8 8 25 81 START lt S2 S2 PR RightIR 1 Z Five lt S3 35 PRR RightIR 1 gt SX D DEUS ce 82 Beam exactly like Toggle Mode records BORK RK kk Ck Ck kk kk Ck KR kk Ck KK kk ck ck ck kk ck ck ck k k ck k ck k kk k ok k ko k k N BEAM 6 BREAK 5 8 26 81 START lt S2 82 PRR RightIR 2 Z Six gt S3 3 PRR RightIR 2 gt SX DD 3 8 2 NCOKCKCk RK kk Ck Ck KK KKK KK Ck kk ko kk ck KK 2 BEAM 7 BREAK NCOKCKCk KK kk Ck kk kk kk kk kkk kkk ck ck ck kck ck k ck k kk k ok k k ck k KK k S 8 27 S1 START gt S2 S25 fR RightIR 3 Z Seven lt S3 S3 FR RightIR 3 SX 0 01 82 BORK RK kk kk kk kk Ck KR kk Ck KR kk ck ck ck k ck ck ck ck k kk k ck k k k ck k k k ck ck X BEAM 8 BREAK NCKCKCKCKCKKCKCKKCKCKKCKCK EO II k kc k Kk kok ck kk kk ok kk S SUB 81 START lt S2 82 PR RightIR 4 Z Eight lt S3 3 PRR RightIR 4
5. gt SX 0 01 2 DESCRIPTION OF STATE SET 32 State Set 32 counts the time for the session NCOKCKCk Ck kk kk kk ke kk kk kk kk kk kk ck ck ck kck ck k ck k kk k k k k ck k k ko kck k N SESSION TEST TIMER 8 8 32 S1 START lt 2 S2 Wait for Start of First Trial 2 Start CS S3 S3 Increment and Display time only Session End is not triggered in S S 1 S5 until Current Trial is completed 1 ADD E SHOW 5 Elapsed Time E 60 SX Z EndSession lt 1 CHAPTER 5 Understanding the Data Files Data can be saved manually by selecting FILE SAVE DATA MANUALLY or FILE SAVE DATA FLUSH The file name that is used to save the data in depends on the option that was chosen in the Hardware Configuration Utility and may also be dependent on the Subject Experiment and Group name provided in the MED PC load wizard Within each data file the headings are created for each Subject Experiment Group Box etc see below Data files may be opened with note pad word pad or any word processor or spreadsheet however be sure they are always saved unformatted in case a data extraction utility such as MED PC to Excel might ever be used Data file formats are explained in detail in the MED PC User s Manual CHAPTER 6 D
6. When the ITI Period ends the Door opens the Tone turns on on the side of the chamber that the subject is on and the Trial begins The trial begins with the Avoid Interval default five seconds If the subject crosses over to the other side of the chamber during the Avoid Interval an Avoid is recorded to the data file and the Trial ends If the subject does not cross over to the other side of the chamber before the end of the Avoid Interval the Escape Interval default ten seconds begins and if the CS UCS overlap is set to the default value of 1 the Tone will stay on until the aversive stimulus turns off When the Escape Interval begins the aversive stimulus turns on If the subject crosses over to the other side of the chamber during the Escape Interval and Escape is recorded to the data file the aversive stimulus turns off and the Trial ends At the end of the Escape Interval if the subject has not cross over to the other side of the chamber the aversive stimulus is turned off a Failure to Escape is recorded in the data file and the Trial ends When the Trial ends the Door closes and the Tone turns off Then another ITI Period followed by another Trial begins until the Number of Trials to Run default 30 trials has been reached When the Number of Trials to Run is reached the experiment is over the Lights turn off and the Door closes Independent Variables 7 Default Variable Name Description
7. Z2 Six ADD B ITIActivity D I 11 gt S10 Z Eight ADD B ITIActivity D I 11 S12 S12 Movement following a Beam 8 Break Z EndSession lt S1 Z Start CS 82 Z Five ADD B ITIActivity D I 11 lt S9 Z Six ADD B ITIActivity D I 11 gt S10 Z Seven ADD B ITIActivity D I 11 gt S11 DESCRIPTION OF STATE SETS 5 amp 6 State Set 5 calculates the response latency variables and prints the information in the data file State Set 6 prints the summary information to the MED PC Run Time Screen FAKK kkk Ck ck KK kk kk kk kkk kkk ck Ok ck kck ck k ck k kk k k k k ck k k kk k k N RESPONSE LATENCY DETERMINATION 8 8 5 S1 2 Start CS S2 S2 Z Avoid SET B AvoidLatency B AvoidLatency D I 8 D I 10 0 SET B EscapeAdjusted B EscapeAdjusted 2 1 12 lt S1 Z Escape SET B EscapeLatency B EscapeLatency D I 10 D I 8 2 SET B EscapeAdjusted B EscapeAdjusted 2 1 12 lt S1 Z Start_ITI SET B EscapeAdjusted B EscapeAdjusted D I 12 SET D I 8 0 D I 10 0 gt S1 No Response 0 01 SET D I 8 D I 8 qe ule N Avoid Latency SET D I 10 D I 10 0 01 Escape Latency SET D I 12 D I 12 0 01 gt SX Adjusted Escape Latency SHOW TRIAL AND SUMMARY DATA A KK CK KC KO KK KK K K KK KK KO KK KO KK OK CK KG S S 6 S1 START CLEAR 1 60 lt S2
8. 82 1 SHOW 1 Trial B TrialCount 2 Avoids B TotalAvoids 3 Escapes B TotalEscapes SHOW 4 Failures B TotalFailures 6 ITI Activity B ITI Activity 9 Crossings B TotalCrossings SHOW 10 Adj Escape Lat B EscapeAdjusted SHOW 11 Left Movement B LeftMovement 12 Left Time B LeftTime SHOW 16 Right Movement B RightMovement 17 Right Time B RightTime lt SX Z EndSession lt 1 DESCRIPTION OF STATE SETS 7 amp 8 State Sets 7 and 8 print avoid and escape latency data to the Run Time Screen BORK RK RR KR KK RK KKK KOK KK RK KK ck ck KK kkk kk kk ck k k kk k k SHOW MEAN AVOID LATENCY 5 7 81 START lt S2 82 1 IF B TotalAvoids gt 1 gt S3 3 1 SHOW 7 Avoid Latency B AvoidLatency B TotalAvoids gt SX Z EndSession lt S1 NCC CKCkCk ok Ck Ok kk kk OK kk Ck Ck Ck Ck Ck kck ck Ck ck k k Ck ck ck k k k kk k k kck k ok ok kk N SHOW MEAN ESCAPE LATENCY 8 8 8 SI START lt 2 S2 1 IF B TotalEscapes lt 1 lt S3 53 1 SHOW 8 Escape Latency B EscapeLatency B TotalEscapes gt SX Z E
9. Excel Nt 29 Using a Pre Formatted Table Profile MTP file 29 Editing the MEP 32 MED ASSOCIATES INC SOF 700RA 28 SHUTTLE BOX LEARNED HELPLESSNESS CHAPTER 1 Introduction The purpose of this manual is to give an explanation of the MED State Notation Procedures that comprise the SOF 700RA 28 Shuttle Box Learned Helplessness Procedures The files in this package can be found on the disk provided by MED Associates Inc These procedures are intended to be run in MED Associates MED PC software The latest version of MED PC gives researchers the ability to use pre programmed procedures such as these to make hardware control and data collection easy These pre programmed procedures can also be modified to meet the evolving demands of a research protocol Again it is the intent of this manual to explain exactly what these procedures implement and provide guidance into how to interpret what the program code achieves in order to let the user determine how to modify them to match their research protocol demands The manual provides some examples of editing and modifying the procedure s programming code The manual also defines the elements in the raw data file produced by these procedures In addition to this manual refer to the MED PC User s Manual for the installation of the MED Associates interface drivers the MED PC Software and the Delphi Compiler Also refer to the User s Manual for instructions on deve
10. SETS 21 28 State Sets 21 28 record the IR inputs for each inputs NCOKCKCk KK kk Ck Ck KK kk kk kk kk KK kk ck ck ck k ck ck k ck k kk k ok k k ck k k k k kk N BEAM 1 BREAK 8 8 21 SL START gt 2 S2 fR LeftIR 4 Z One S3 S3 FR LeftIR 4 SX 0 0 1 s IE B2 A E E E a ok CK kk Ck Ok E E kk Ck Ck Ck Ck I ck k k k kk k k kck ck kok kk X BEAM 2 BREAK 8 8 92 81 START lt S2 82 PRR LeftIR 3 Z Two gt S3 S3 PRR LeftIR 3 gt SX 0 01 S2 A E E E a ok Ck Kk Ok kk Ok kk Ck kk Ck II k k k k k k ck kck k ok ok ko X BEAM 3 BREAK Sos dy 81 START lt S2 82 fR LeftIR 2 Z Three gt S3 3 PRR LeftIR 2 gt SX 0 01 2 gt ok Ck Ok kk kk Ok kk Ck Ck Ck Ck I ck k k k kk k k kck k ok ok ko X BEAM 4 BREAK NCKCKCKCkCk Ck Ck Ck kk Ck Ck Sk kk ke kk kk kk Ck Ck Ck kk Ck kk kc kk kk kc k kc k kk ke k 8 898 24 81 START
11. This screen allows the user to alter the parameters by which a procedure executes Make any desired changes to the session parameters and click Next to proceed Figure 3 7 Alter Session Parameters Screen Acclimation Period min Number of Trials to Run Avoid Interval sec Escape Interval sec CS UCS Overlap 1 Yes 0 No The next screen to appear is the Send Start Command screen The options available on this screen vary depending upon the number of boxes entered in the Hardware Configuration Utility refer to the MED PC IV User s Manual for more information regarding the Hardware Configuration Utility In this example there is only one box attached to the interface so Figure 3 8 will appear next If more than one box is attached to the interface then Figure 3 9 will appear next Figure 3 8 Send Start Command Screen for Single Box Configuration 11 MED ASSOCIATES INC SOF 700RA 28 SHUTTLE BOX LEARNED HELPLESSNESS Figure 3 9 Send Start Command Screen for Multiple Box Configuration In both cases Figure 3 8 and Figure 3 9 this screen is where the user decides to either load more boxes send a start signal to boxes that are already loaded or enter the MED PC run time environment without sending a start signal by selecting I am finished with the wizard Make a selection and click Continue For the purposes of this example I am finished with the wizard was selected This opt
12. and the ITI Period begins when either the Animal crosses to the other side or the Escape Interval ends When the Trial ends the Shock will be turned off After the ITI Period randomly drawn from a list ends the Door will open again and the next Trial will start EE GO EGO GP GP GE PG GEO GPO GPG ut Program runs only in Level Mode The 1 Beam is the Beam Closest to the Door or Hurdle on each Side Inputs are Assigned from Left to Right as viewed from front of Box Inputs LeftIR 4 LeftIR 3 LeftIR 2 LeftIR 1 RightIR 1 RightIR 2 RightIR 3 RightIR 4 Uo gy gw dg wg dw dH og co 41 0 01 ES Outputs LeftLight RightLight LeftTone RightTone Door ShockOperate LeftGrid RightGrid Uo wg gw gp dw dw dg 10 01 5 00 S Control Variables with Assigned Aliases as Defined Var Alias Acclimation Period min A 0 Default 2 minutes Var Alias Number of Trials to Run A 1 Default 30 Var Alias Avoid Interval sec A 2 Default 5 seconds Var Alias Escape Interval sec A 3 Default 10 seconds Var Alias CS UCS Overlap l Yes 0 No A 4 Default Yes Tone is On During Shock MED ASSOCIATES INC SOF 700RA 28 SHUTTLE BOX LEARNED HELPLESSNESS Subscript Constants for Control Variables Acclimation 0 Trials CS Time UCS_Time OverlapTag BWN IH List Data Variables Here B Summary Data Array B 0 Tot
13. of the programs in case of disk failure Having copies of the original programs may be useful in the future should modifications be made to the existing programs CHAPTER 3 Beginning amp Running an Experiment Translating the MED PC mpc File Programs written in MedState Notation must be translated using Trans IV before they can be executed in this application Open Trans IV icon and select Translation Translate and Compile Select the program s to use for the experiment and click Make Click OK to start the translator and it will automatically parse the MedState Notation and then open to a DOS screen to compile the Pascal code Depending on the speed of the computer each of these steps may not be seen If any problems are encountered during this process refer to the on screen help menu or the MED PC User s Manual or contact MED Associates Inc for assistance Figure 3 1 Trans IV Control Panel for Translating and Compiling MedState Notation Code Gs Specify Files to Jranslate BAE Helpl T Shuttle Box Learned Helplessness Training mpc Make Build No Trans Exclude X Cancel Help MED ASSOCIATES INC SOF 700RA 28 SHUTTLE BOX LEARNED HELPLESSNESS Using the MED PC Load Wizard MED PC is designed to help the researcher run an experiment by guiding selection choices through its Experiment Loading Wizard This section will describe how to initiate the Shuttle Box Learned Helplessness Test mp
14. the products and services we provide and look forward to meeting your future experimental needs as your research program evolves Overview of Shuttle Box Learned Helplessness Training mpc The subject should be loaded into either side of the chamber When a START command is issued the program detects whether or not the subject has been loaded into the chamber If so the experiment begins with the Left and Right lights turning on and the door opening Then the ITI Period begins which is randomly drawn from a list and will be between two and 15 seconds in duration When the ITI Period ends the Tone turns on for the Tone Duration default five seconds then the Aversive Stimulus duration is randomly selected from a list and varies between one and ten seconds will be delivered The program will alternate between the ITI and the Tone Aversive Stimulus until the specified Number of Trials to Run default 180 has been delivered Overview of Shuttle Box Learned Helplessness Test mpc The subject should be loaded into either side of the chamber When a START command is issued the program detects whether or not the subject has been loaded into the chamber If so the Left and Right lights turn on the door opens and the Acclimation Period default two minutes begins Following the Acclimation Period the door closes for the length of the ITI Period which is randomly drawn from a list and will be between two and 15 seconds in duration
15. wizard setup application S2 starts the procedure on animal entry S3 times the acclimation period S4 waits for the end of each trial S5 times the ITI S6 sets up a new trial S7 and saves the data S10 13 A A E E a KK kk Ck KK Ck kk Ck Ck Ck kk kck Ck Ck ck k k k ck ck k k k ok k k k ck Ck k ok ck ck ck ko k Shuttle Box Learned Helplessness Test Schedule 81 Set Default Values N Acclimation Period 2 minutes N Number of Trials to Run 30 N Avoid Interval 5 seconds N Escape Interval 10 seconds N CS UCS Overlap Yes Ok kk Ck ck k k kk ck k k k ok k k ck ck ck ck ck ck ck ck ck k S S 1 Setup Adaptation amp ITI Interval Timers S1 0 001 SET A Acclimation 2 A Trials 30 A CS Time 5 SET A UCS Time 10 A OverlapTag 1 gt 2 S2 START SET A CS Time A CS Time 1 SET A UCS Time A UCS Time 1 SET D I 13 987 987 ON LeftLight RightLight gt 3 1 SHOW 1 Acclimation A Acclimation 2 Trials A Trials 3 Avoid Time A CS Time SHOW 4 Escape Time A UCS Time 5 CS UCS Overlap A OverlapTag lt SX 83 Verify Detection of Animal 0 01 IF L 1 OR L 2 GStartNow Wait Start ON Door Z Start A gt S4 Wait gt SX S4 Time Acclimation Period 1 ADD E SHOW 5 Acclimation Time E 60 IF E 60 gt A Acclimation Done Cont Done OFF Door RAND
16. 22m Subject 0 dat 3 2007 09 28 1 5h50m Subject Subject 1 csv Desktop E Graphics_Subject1_Experiment1 5 Graphics Window Subjecti Groupi 7 SI_Experiment_Subject1_Group1 s Two Chamber Place Pref Auto Doors mtp gt Two Chamber Place Pref Man Doors mtp My Documents My Computer File name 2008 08 22 dat Files of type am File Cancel Open as read only 31 Editing the MTP file The MTP file can be edited to customize the transfer process and display the data of most interest See the User s Manual for MPC2XL for explicit instructions about how to modify the MTP file using the Edit Table Profiles tab shown in Figure 6 5 Header Titles are user defined and can include any information that will help label the data listed below the title Header Elements are the data points that will get transferred from the raw data file into Excel The raw data file will list the elements that can be included in the MTP file e g A Z Figure 6 5 Edit Table Profiles MED PC to Excel File Help Transfer Data Edit Row Profiles Edit Table Profiles Edit Mode 1 2 8 4 H Header Titles Em subject Experimer Group Comment Header Elements IStartDate Subject Experimer Group Comment Header Titles Total Trial Left Mvmt Right Mvrr Crossings Failures Left Time Header Elements B0 B 1 B 2 B 3 B 4 B 5 Column Titles Trial Numt Left Mvmt Right Mvrr Crossings Failures Left
17. 6006 Trial Data Array ITI Intervals For both the Training and Test programs the ITI Intervals are randomly chosen from a list in the program The list must be defined as real numbers times and can be user defined The default values are shown below Available ITI Intervals LIST Y 2 9g 4 6 p 8 9 10 11 12 13 14 15 For the Training program the aversive stimulus durations are also randomly chosen from a list in the program The list must be defined as real numbers times and can be user defined The default values are shown below Available Aversive Stimulus Lengths LIST T ET 2 3 4 DT 6 a pim 9 10 Array Columns and Number Format Set the Array Columns and Number Format in ASCII Data File where the columns are 6 and the number format is DISKCOLUMNS 6 DISKFORMAT 6 2 CHAPTER 2 Getting Started Software Installation Please refer to the MED PC User s Manual for a complete guide to installing the MED PC software building a valid Hardware configuration with the Hardware Configuration utility and opening and compiling a MSN procedure in the Trans IV utility To install the Shuttle Box Learned Helplessness Procedures insert the CD into the CD ROM drive and click Install the Shuttle Box Learned Helplessness Software The procedures are copied into the indicated folder Backing Up the Software Med Associates strongly encourages creating backup copies
18. D M Y SET E 0 Z Start ITI gt S6 Conti gt S4 Z EnterLeft Z EnterRight ADD D I 3 B TotalCrossings lt SX S5 N Wait for End of Trial Z2 Avoid Z Escape Z Start ITI OFF Door lt S6 S6 Time ITI Interval End Session following completion of ITI if Session Time or Number of Trials completed is equal to or greater than the set values M T IF B TrialCount gt A Trials EndSession NewTrial End Z EndSession gt S10 New ON Door gt S7 S7 Set up new Trial 0 01 ADD B TrialCount SET I I 13 SET D I B TrialCount D 1I 13 987 987 SHOW 1 Trial D I RANDD M Y Z Start CS lt S5 S10 V End of Session Calculations Check for Zero Avoids 0 01 IF B TotalAvoids 0 True False True gt S11 False SET B AvoidLatency B AvoidLatency B TotalAvoids gt S11 Sil Check for Zero Escapes 0 01 IF B TotalEscapes 0 True False True gt S12 GFalse SET B EscapeLatency B EscapeLatency B TotalEscapes gt 12 12 Calculate the Adjusted Mean Escape Latency 0 01 SET B EscapeAdjusted B EscapeAdjusted B TrialCount gt 3 513 Delay for Screen Update 2 OFF Door STOPABORTFLUSH DESCRIPTION OF STATE SET 2 State Set 2 tracks the location of the animal in the chamber 0200 RK KK KR KOK KK KOK KK RK KK k ck ck k ck k kk k ok k k ck k k k k kk N TRACK ANIMAL LOCATION OK OK ok CK kk
19. S4 Z Seven ADD B RightMovement 2 1 2 lt S6 Z Eight ADD B RightMovement D I 2 S7 S6 Movement following a Beam 7 Break Z EndSession 25 gt 1 Z Five ADD B RightMovement 2 1 2 lt S4 A sd ADD B RightMovement 2 1 2 lt S5 Z Eight ADD B RightMovement 2 1 2 lt S7 S7 Movement following a Beam 8 Break Z EndSession cm 1 Z Five ADD B RightMovement 2 1 2 lt S4 Z Six ADD B RightMovement 2 1 2 lt S5 Z Seven ADD B RightMovement 2 1 2 lt S6 DESCRIPTION OF STATE SET 12 State Set 12 records the amount of time spent on each side of the chamber BORK RK kk Ck Ck kk kk Ck KK kk Ck KR kk ck ck ck kk ck ck ck k kk k ck k k k ko k k k ck ck X COUNT TIME ON EACH SIDE 9 85 12 Sie Verify Detection of Animal START lt 2 2 0 01 IF L 1 Left Next Left gt S3 Next IF L 2 Right Wait Right gt 4 Wait gt BX S3 N Animal is on the Left 0 01 SET B LeftTime B LeftTime 0 01 SET D I 5 D I 5 SO 0T m c53 Z EndSession lt 1 Z EnterRight lt S4 S4 Animal is on the Right 0 01 SET B RightTime B RightTime 0 01 SET D I 6 D I 6 0 01 4 2 EnaSession lt 1 Z EnterLeft S3 DESCRIPTION OF STATE
20. SHUTTLE BOX LEARNED HELPLESSNESS MED STATE NOTATION PROCEDURE SOF 700RA 28 Manual DOC 235 Rev 1 0 Copyright 2010 All Rights Reserved MED Associates Inc P O Box 319 St Albans Vermont 05478 www med associates com Trademarks MedState Notation MED Associates Inc Registered Trademark MED PC MED Associates Inc Delphi Borland International Inc MED ASSOCIATES INC SOF 700RA 28 SHUTTLE BOX LEARNED HELPLESSNESS TABLE OF CONTENTS Chapter 1 mi 1 1 Overview of Shuttle Box Learned Helplessness Training mpPC ss 2 Overview of Shuttle Box Learned Helplessness Test mMpC 2 Inp ts and Outputs 4 MISC 5 Chapter 2 0 6 Getting Started sa iva ss p una abies oe ENAP EEU PEATA NE tasted 6 Software Installation 6 Backing Up the 9oftWware reiner pa eR ERE PARRA SERRE X ERR EAKEKE SE ERRARE FAR ERE EE 6 Chapter 3D 7 Beginning amp Running an Experiment ns 7 Translating the MED PC mpc File tt 7 Using the MED PC Load Wizard 8 Viewing Changing Variable Values Ne 13 MACOS code aa EAEEREN AA TAERAA RREA 15 Modifying the MedState Notation Code 4 16 Chapter 4 om 17 Modifying the Existing Program sei ms 17 Introduction Shuttle Box Learned Helplessness Test mpc sseseseeesesse 17 Chapter Dai 28 eee 28 Chapter Ov a ONU NR EAR ER AR RR EER ARR ER E ER AERE REA ARR NER ANKE EEK KE 29 Data Analysis Using MED PC to
21. Shuttle Box Learned Helplessness Test mtp will be used Figure 6 2 Select File to Open Look in Data 2 Shuttle Box Learned Helplessness Test mtp Shuttle Box Learned Helplessness Training mtp My Recent Documents 3 f 2 My Documents My Computer File name Shuttle Box Learned Helplessness Test mtp ked My Network Files of type MED PC Table Profile Places Open as read only 3 Note that MTP file is listed under the Table Transfer Profile Select Labels and Data because selecting these options will print data labels as well as import data Click Transfer Figure 6 3 Transfer Data MED PC to Excel File Help Transfer Data Edit Row Profiles Edit Table Profiles Row Transfer Profile NO PROFILE ASSIGNED Select Transfer Orientation Column Labels Horizontal Rows iv Data C Vertical Columns Table Transfer Profile CAMED PC IV Data Shuttle Box Learned Helplessness Test mtp Transfer labels Transfer SS iv Data 30 MED ASSOCIATES INC SOF 700RA 28 SHUTTLE BOX LEARNED HELPLESSNESS 4 Specify the raw data file to transfer and then click Open This step performs the transfer and now the data has been sent to Microsoft Excel Figure 6 4 Specify Data Files to Transfer Specify Data Files to Transfer l Look in O Data Be E3 E 2006 09 01 t 2006 09 05 My Recent 7 Documents 2006 09 13 3 t 2007 07 16 14h
22. Time 1st Column Element D0 D 1 D 2 D 3 D 4 D 5 Increment 13 13 13 13 13 13 Last Column Element D 5984 D 6995 D 5996 D5997 D 5998 D 5999 Profile in Editor CAMED PC IMData Shuttle Box Learned Helplessness Test mtp Create Table Create Records Create Freestyle To edit either the Header Titles or Header Elements click on the appropriate cell in the Edit Table Profiles window Rows and columns can be added to the file First select the desired location then right click to add either the desired row or column Use the right click option titled Paste an Identifier to include subject or session identifying information Note that when using the Paste an Identifier function Header Titles and Header Elements are edited and pasted automatically To save the edited MTP file select Save and create a new filename in the Data subfolder To use this newly edited and saved MTP file verify that the file is selected in the Table Transfer Profile display Figure 6 3 and then click Transfer
23. Value Acclimation Period Amount of time for the subject to acclimate to the 2 minutes chamber Number of Trials to Run Variable defines the number of trials A trial ends and 30 the ITI period begins when either the subject crosses to the other side of the chamber OR the Escape Interval ends whichever happens first Avoid Interval seconds Maximum duration of the conditioned stimulus CS 5 prior to the delivery of the unconditioned stimulus UCS In this case the CS is the tone Escape Interval Maximum duration of the UCS In this case the UCS is 10 seconds the aversive stimulus The UCS will terminate after the subject has crossed over to the other side of the chamber or when this time has elapsed whichever happens first CS UCS Overlap If this variable is set to 0 the CS tone stimulus is 1 1 Yes 0 No terminated at the onset of the UCS aversive stimulus If this variable is set to 1 then the CS and the UCS will terminate in unison Dependent Variables Avoids The number of times that the subject crosses over to the other side of the chamber before the end of the Avoid Interval Escapes The number of times that the subject crosses over to the other side of the chamber before the end of the Escape Interval Crossings A crossing is defined as the subject moving from one side of the chamber to the other Failures to Escape The number of times that the subject does not cross over to the other
24. al Trials Run B 1 Total Movement in Left Chamber B 2 Total Movement in Right Chamber B 3 Total Number of Crossings Total Failures to Escape Total Time spent in Left Chamber B 6 Total Time spent in Right Chamber B 7 Total Number of Avoids B 8 Mean Avoid Latency N N N N N N N t Xo B45 N N N N N N N B 10 1 2 B 9 Total Number of Escapes Mean Escape Latency B Total ITI Activity B Adjusted Mean Escape Latency Subscript Constants for Summary Data Array TrialCount 0 LeftMovement 1 RightMovement 2 TotalCrossings 3 TotalFailures 4 LeftTime 5 RightTime 6 TotalAvoids 7 AvoidLatency 8 TotalEscapes 9 EscapeLatency 10 ITIActivity 11 EscapeAdjusted 12 D Trial by Trial Data Array D I Trial Number D I 1 Left Movement Activity D I 2 Right Movement Activity D I 3 Crossings D I 4 Failure to Escape Tag D I 5 Left Time N D I 6 Right Time D I 7 Avoid Tag D I 8 Avoid Latency D I 9 Escape Tag D I 10 Escape Latency D I 11 ITI Activity D I 12 Adjusted Escape Latency Defined as no crossing while the Shock is Divided by Total Avoids for Mean Latency Divided by Total Escapes for Mean Latency Trial 0 is the Acclimation Period 18 Et 1 Working Variables Here E Elapsed Time in Seconds for Acclimation Period and Session 1 Subscrip
25. ata Analysis Using MED PC to Excel Using a Pre Formatted Table Profile MTP file MED PC to Excel MPC2XL is a program that helps to import data from MED PC the raw data file format previous section to a spreadsheet program such as Microsoft Excel MPC2XL needs to be installed separately from MED PC Please refer to the User s Manual for MPC2XL for installation instructions Once MPC2XL is installed open the folder that the program was saved to and the MTP files have been saved in the C MED PC IV Data folder Follow the step by step instructions below for importing data 1 Open Microsoft Excel and then minimize the window Open MED PC to Excel and the display shown in Figure 6 1 will appear The uppermost file display should be titled Transfer Data Under the Table Transfer window at the bottom of the screen click on Select Figure 6 1 Table Transfer MED PC to Excel BEI File Help Transfer Data Edit Row Profiles Edit Table Profiles Row Transfer Profile NO PROFILE ASSIGNED Transfer Orientation Column Labels Horizontal Rows Tranefen Data C Vertical Columns Table Transfer r Profile NO PROFILE ASSIGNED set umm Transfer 34 T Labels Transter 2 Choose the MTP file in the C MED PC IV Data folder that corresponds to the MED State Notation Procedure that was run and click Open For this example
26. by 1 Click the Issue button for the change to take effect Click Named Variables to produce the display in Figure 3 13 Change variables here as needed MED ASSOCIATES INC SOF 700RA 28 SHUTTLE BOX LEARNED HELPLESSNESS Figure 3 12 Displaying Array A from Box 1 Displaying Array A from Box 1 A AQ AG A 4 E Named Vars 2 30 000 500 000 1000 000 1 000 Yars Refresh Issue Close rd a Help Additional Boxes to Update JACO from Box 1 1 Select All Deselect All P 000 Figure 3 13 Displaying Named Variables from Box 1 Displaying Named Variables from Box 1 Acclimation Period min 1 Named tare Number of Trials to Run 30 000 Avoid Interval sec 5 000 Yars Escape Interval sec 10 000 CS UCS Overlap 1 Yes 0 No 1 000 Issue Close Help Mul Additional Boxes to Update Display Data from Box rea Select All Deselect Al gr E sige Macros The simplest way to initially create a macro is to record keyboard functions while performing the steps manually Once the commands are in the macro it is easy to create a number of macros with the macro editor The following example illustrates the process of loading Box 1 and changing the Number of Trials to Run to 20 To begin open MED PC and going directly to the run time screen Close the load wizard if present Before loading or
27. c application however the following steps apply to all other mpc procedures Open MED PC and the MED PC Experiment Loading Wizard s Welcome screen shown in Figure 3 2 will appear Figure 3 2 The MED PC Loading Wizard Welcome Screen To avoid this load wizard deselect the checkbox labeled Run this experiment automatically when starting MED PC Close this screen by clicking the Close button Closing this screen immediately reveals the MED PC Run Time Screen shown in Figure 3 10 If the choice to continue with the Loading Wizard is made then click the Next button MED ASSOCIATES INC SOF 700RA 28 SHUTTLE BOX LEARNED HELPLESSNESS The Box Selection screen will appear next as shown in Figure 3 3 From this screen the researcher chooses which boxes will be used in the experiment Select the boxes that will run the experiment by clicking in the radio button next to the box number The figure shows that the Hardware Configuration included only 1 box which was selected Click Next to continue Figure 3 3 The Box Selection Screen The Select a Procedure screen appears next shown in Figure 3 4 This screen displays a list of all the currently compiled procedures Highlight the desired procedure and click Next Figure 3 4 The Select a Procedure Screen SHUTTLE BOX LEARNED HELPLESSNESS TEST SHUTTLE BOX LEARNED HELPLESSNESS TRAINING MED ASSOCIATES INC SOF 700RA 28 SHUTTLE BOX LEARNED HELPLESSNESS Next the Ent
28. er Experiment Data Screen will appear shown in Figure 3 5 This screen allows the user to add annotations to the data file that is produced by MED PC These annotations will help identify the Subject Experiment and Experiment Group from which data was collected Comments can be added here as well and the data file can be given a customized file name to help identify it from other data files Enter the information desired and click Next Figure 3 5 Enter Experiment Data Screen Subject lect Experiment Fe 8 G roup Group 1 Comments Shuttle Box Leamed Helpessness Test Optional Custom Filename Subjectt_Group _ShutleBoxTes GE The next screen to appear is the Review Choices screen shown in Figure 3 6 This screen allows the user to confirm that the information that was entered on previous screens If it is not correct click Previous to go back to the previous screen and edit the information If it is correct click Next to proceed Figure 3 6 Review Choices Screen Review the data below Box 1 Procedure SHUTTLE BOX LEARNED HELPLESSNESS Subject Subject 1 Experiment Experiment 1 Group Group 1 Comment Shuttle Box Learned Helpessness Test Filename Subjectl Groupl ShuttleBoxTest Click Next to finish loading the box or Previous to correct errors 10 MED ASSOCIATES INC SOF 700RA 28 SHUTTLE BOX LEARNED HELPLESSNESS The next screen to appear is the Alter Session Parameters Screen shown in Figure 3 7
29. for a specified number of trials default 30 Shuttle Box Learned Helplessness Test mpc is listed below in its entirety followed by a brief explanation of each state set See the MED PC IV User s Manual for details regarding the processing of commands in MED PC The MED PC IV Programmer s Manual and online tutorial provides necessary information on Med State Notation and these resources will be very helpful when making adjustments to Shuttle Box Avoidance Level mpc or creating a custom mpc program Shuttle Box Learned Helplessness Test mpc Copyright C 2010 MED Associates All Rights Reserved Shuttle Box Learned Helplessness Test mpc When the program is STARTed the Left and Right lights will be turned on The Animal can be loaded into the chamber either before or after the START command has been issued Once the program has detected that the Animal has been loaded and the START command has been issued the Door will open and the Acclimation Period will begin The program will start with the Acclimation Period After the Acclimation Period ends the Door will be closed for the length of the ITI Period randomly drawn from a list When the ITI Period ends the Door will be reopened and the Tone will be turned on on the side that the Animal is located The Animal will have the amount of time specified in the Avoid Interval to Avoid the shock and the amount of time specified in the Escape Interval to Escape from the Shock The Trial ends
30. huttle Box Learned Helplessness procedures can be made to the MedState Notation code To make the same change to the Number of Trials to Run as shown above do the following Open Trans IV and select File Open to place Shuttle Box Learned Helplessness Test mpc into the text editor Scroll down to approximately line 167 note the line counter on the left side of the editor to reveal the code shown in Figure 3 15 Figure 3 15 Shuttle Box Learned Helplessness Test mpc Line 167 EX TRANS IV C MED PC IV MPC Shuttle Box Learned Helplessness Test mpc A File Edit Search Options Translation Window Help ee x e ole Qla Alas S 8 1 Setup Adaptation 6 ITI Interval Timers 1 0 001 SET A Acclimation 2 A Trials Hg ics Time 5 1 82 SET A UCS Time 10 A OverlapTag 2 HSTIRT SFT i CS Time bi CS Time 1 Change A Trials 230 to A Trials 220 and save the changes with the same or a new file name such as Shuttle Box LH Test 20 Trials mpc Remember if creating a new mpc file name and are using a macro to load boxes the file name in the macro also must be changed Translate and compile the new or changed file as described previously and run MED PC Use the Change Variables screen to view confirm the new values CHAPTER 4 Modifying the Existing Program Introduction Shuttle Box Learned Helplessness Test mpc The Shuttle Box Learned Helplessness procedure was written to run
31. ion results in the MED PC IV run time screen appearing as shown in Figure 3 10 Figure 3 10 The MED PC Run Time Screen MED PC IV ojx File Configure View Macros Help Aloe Ax alt Ja om 4 sista A 9 1 2010 9 06 13 AM EMULATION MOC 4 12 Viewing Changing Variable Values Once a session is in progress the session parameters can be changed by selecting Configure Change Variables or click the 4th tool bar item AX In the lower left hand corner of the Change Variables window find the Display Data from Box display and choose the chamber s to modify By clicking additional boxes in the Additional Boxes to Update section changes made to a single box are automatically loaded to all of the selected boxes Figure 3 11 Changing Variables Screen Displaying Variables from Box 1 A Array B Array oe D Array E o0 T Nanedvas F G H k n oye 0 000 0 000 0 000 0 000 0 000 000 T6000 icis 11000 0000 000 Issue 0 000 000 000 000 Y Array Close 6000 Help Display Data from Box Additional Boxes to Update C from Box 1 t eH C2 1 r2 0000 Select All Deselect All The value of any simple variable may be viewed from this screen by clicking an array on the table and each element in that array can be viewed as shown in Figure 3 12 To change a value simply highlight and replace the value in the lower right hand box or use the up down arrows to increment
32. loping a Hardware Configuration Data file structure file saving format and other related options are also determined by the Hardware Configuration Running the Hardware Configuration software utility that accompanies MED PC sets the Hardware Configuration Its purpose is to assign the inputs and outputs on the interface cards in the interface cabinet for each task controlled by MED PC The particular type of interface card that is supplied in the interface cabinet may vary please refer to the User s Manual provided for instructions on how to configure the type of card that is in the cabinet A valid Hardware Configuration must exist in order for MED PC to interface correctly with the MED Associates Inc hardware This means that one should take the time to create a valid Hardware Configuration before attempting to run the procedures included in this package Should there be any problems the staff at MED Associates Inc is available to answer any questions that may arise Please e mail us at support med associates com with a detailed description of the problem or desired goals so that concise and detailed information may be provided The Shuttle Box Learned Helplessness procedures are designed to be as easy to use as possible MED Associates Inc understands that researchers do not have the time to devote to programming and hardware design and for that reason we have undertaken that burden for you We sincerely hope that you are satisfied with
33. lt S1 Z Start CS 82 A CFOUT ADD B ITIActivity D I 11 gt 4 Z Two ADD B ITIActivity D I 11 lt S6 Z One ADD B ITIActivity D I 11 lt S7 S6 Movement following a Beam 3 Break Z EndSession lt S1 Z Start CS 2 Z Four ADD B ITIActivity 2 1 11 gt S4 Z Three ADD B ITIActivity D I 11 lt S5 Z One ADD B ITIActivity D I 11 lt S7 S7 Movement following a Beam 4 Break Z EndSession lt S1 Z Start CS 2 Z Four ADD B ITIActivity D I 11 lt S4 Z Three ADD B ITIActivity 2 1 11 gt S5 Z Two ADD B ITIActivity D I 11 lt S6 Animal on Right S8 N Wait for first Beam Break Z EndSession lt S1 Z Start CS 5 82 End of ITI Z Four 9 Z Three mcm 810 Z Two cec 1 Z One gt S12 S9 Movement following a Beam 5 Break Z EndSession lt S1 Z Start CS 2 Z Six ADD B ITIActivity D I 11 lt S10 Z Seven ADD B ITIActivity D I 11 gt 1 Z Eight ADD B ITIActivity D I 11 lt S12 S10 Movement following a Beam 6 Break Z EndSession lt S1 Z Start CS S2 Z Five ADD B ITIActivity D I 11 gt S9 Z Seven ADD B ITIActivity D I 11 lt 1 Z Eight ADD B ITIActivity D I 11 lt S12 22 Sit Movement following a Beam 7 Break Z EndSession lt S1 Z Start CS gt 2 Z Five ADD B ITIActivity D I 11 lt S9
34. ndSession lt S1 DESCRIPTION OF STATE SET 9 State Set 9 operates the tone stimulus S3 waits for either the escape avoid response or the end of the escape interval to turn off the stimuli NOKCKCk Ck Ck kk Ck ck KK kk KKK Ck kk kc kk ck ck ck k ck ck ck k k kk k k k k ck k k k k kk N TONE CONTROL 8 8 9 S1 START lt S2 82 Wait for Signal to turn Stimuli ON 2 Start CS IF L 1 Left Right Left ON LeftTone gt S3 Right ON RightTone gt S3 S3 Wait for Signal to turn Stimuli OFF Z End CS OFF LeftTone RightTone lt 2 DESCRIPTION OF STATE SETS 10 amp 11 State Sets 10 and 11 record left and right movement within the chamber respectively E kk Ok kk Ck kk ck k k Ck ck I k k k ck k k kck k ok ok ko N LEFT MOVEMENT ACTIVITY 8 8 10 S1 START lt 2 S2 Wait for Start of First Trial Z Start A S3 S3 N Wait for first Beam Break 2 S4 2 Three lt S5 2 S6 Z One gt S7 S4 Movement following a Beam Break Z EndSession gt 1 Z Three ADD B LeftMovement D I 1 lt S5 2 ADD B LeftMovement D I 1 lt S6 Z One ADD B LeftMovement D I 1 gt S7 S5
35. opening the procedure click Macro on the main menu and select Turn On Macro Recorder or click the 8th tool bar item with the cassette tape icon on top A note on the bottom of the display indicates that the recorder is running Open Shuttle Box Learned Helplessness Test mpc by clicking File Open Session Change the variables using any of the methods described above When all settings have been made turn the recorder off again by using the main menu or tool bar Save the macro with a distinctive name The example in Figure 3 14 was named Shuttle Box LH 20trials mac since the Number of Trials to Run was changed to 20 Figure 3 14 Shuttle Box LH 20trials mac C MED PC IVMacrolShuttle Box LH 20trials MAC File Edit Search Insert 5 LOAD BOX 1 SUBJ 0 EXPT 0 GROUP 0 PROGRAM SHUTTLE BOX LEARNED HELPLESSNESS TES SET Number of Trials to Run VALUE 20 MAINBOX 1 BOXES 1 gt Once this macro is built use the macro editor in MED PC IV to make simple changes such as replacing reward or correct lever values Open the macro editor by selecting Macros Editor Review the Help file on screen or the MED PC Users Manual for more information on macros and the features offered A START command or message box followed by a START command could be added to the macro it was left off here so changes could be verified before starting the procedure Modifying the MedState Notation Code Permanent changes to the S
36. otalAvoids ADD D I 3 B TotalCrossings Z Avoid Z End CS lt 1 12 UCS Period Right Chamber A UCS Time T OFF ShockOperate RightGrid ADD D I 4 B TotalFailures Z Start ITI Z End CS lt 1 Z EnterLeft ADD D I 9 B TotalEscapes ADD D I 3 B TotalCrossings Z Escape Z End CS gt 3 5135 Shock OFF Delay to Force a Complete Crossing 1 5 OFF ShockOperate RightGrid lt 1 DESCRIPTION OF STATE SET 4 State Set 4 counts the ITI activity of the subject NCOKCKCk KK kk kk kk kk kk kk kk KK kk ck Ck ck kck ck k N COUNT ITI ACTIVITY 8 4 S1 START lt S2 S2 Wait for Start of ITI Z Avoid Z Escape 2 Start ITI IF L 1 Left Right Left soc 63 Right gt S8 cnc Lc LM Animal on Left 83 Wait for first Beam Break Z EndSession lt 1 Z Start CS gt 82 End of ITI Z Four lt S4 Z Three gt 5 Z Two gt 6 Z One gt 8 S4 Movement following a Beam 1 Break Z EndSession lt S1 Z Start CS 82 Z Three ADD B ITIActivity D I 11 lt S5 Z Two ADD B ITIActivity D I 11 gt S6 Z One ADD B ITIActivity D I jo d 85 Movement following a Beam 2 Break Z EndSession
37. side of the chamber before the end of the Escape Interval Inputs and Outputs Shuttle Box Learned Helplessness is intended to be used with shuttle box systems that include eight I R Photobeam sensors separate Left and Right Lights a Tone and may include an optional Auto Guillotine Door All Inputs must be set to LEVEL MODE The 1 Beam is the beam closest to the Door or Hurdle on each side counting up to the Z4 beam as beams reach the outside of the chamber Viewing from the front of the chamber the inputs are assigned as shown below Figure 1 1 IRs Numbered Front of the Chamber Ea RIGHT i 3 2 1 1 2 3 g e e e e e 0 e e 0000000000000000000000000000 e Figure 1 2 Interface Panel Connections Labeled SG 716B a OUTPUTS INPUTS OUTPUTS LEFT LIGHT ol LEFT IR 4 RIGHT LIGHT ee LEFT IR 3 a LEFT TR 2 Camii o om o Me LEFT IR RIGHT TONE 4 5 Eehm ai an RIGHT IR DOOR Tor Gan 3 Fowl G 1 SHOCK OPERATE RIGHT IR 2 LILILL NX MALA RIGHT IR 3 srr eno ea e RIGHT GRID stessi spes feos RIGHT IR 4 lep 9r 28 Volts Misc Information Data Array Dimensions The dimensions of the data arrays are shown below They are intended to allow the user to collect a maximum amount of data DIM A 4 Named Variables DIM B 12 Summary Data Array DIMD
38. t for Data Array D L M ct Location Flag 1 Left 2 Right Trial ITI Drawn from List Y Y List of ITI times 2 15 seconds This list may be edited as needed These numbers appear in MED time units i e 1 second 100 MED Ticks given BO adi P PE Gg a Resolution setting of 10 ms Z Pulses Used in This Procedure Start CE 1 Z1 Signal Start of CS Avoid 2 22 Signal Avoid Escape 3 Z3 Signal Escape Start A 4 Z4 Signal Start of Acclimation Period Start ITI 5 25 Signal Start of ITI EnterLeft 6 26 Entrance to Left Compartment must break beam 2 4 EnterRight 7 Z7 Entrance to Right Compartment End CS 8 Z8 Signal no CS UCS Overlap Stim Off EndSession 9 Z9 End of Session One 21 221 Beam Break Two 22 222 Beam 2 Break Three 23 223 Beam 3 Break Four 24 224 Beam 4 Break Five 25 225 Beam 5 Break BIX 26 226 Beam 6 Break Seven 27 VN 227 Beam 7 Break Eight 28 228 Beam 8 Break Dimension Array Sizes DIM A 4 Named Variables DIM B 12 Summary Data Array DIM D 6006 Trial Data Array Available ITI Intervals ST Y 2 3 4 5 6 7 8 9 10 11 12 13 14 15 E DISKCOLUMNS 13 DISKFORMAT 6 2 DESCRIPTION OF STATE SET 1 State Set 1 is the core of program and sets the default values for all independent variables S1 programs the load
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