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1. QuickStix Kit for Cry1A Corn Bulk Grain Rev 02 01 13 Highlights Use with Common Extraction method Quantitative results in just over 5 minutes Available as 100 strip kits in bulk packaging or in QuickCombs Contents of Kit 100 QuickStix Strips packed in two moisture resistant canisters 100 transfer pipettes 100 sample cups Items Not Provided Bunn grinder or equivalent Graduated cylinder Tap water QuickScan System For sampling scenarios at different screening or confidence levels refer to the USDA GIPSA Excel spreadsheet described below or call EnviroLogix Technical Support for assistance Corn Common Extraction Grams of Corn x 1 5 mL of water For example 400 kernels with an average seed weight of 0 3 g 400 x 0 3 120 g of corn 120 g x 1 5 180 mL water Catalog Number AQ 003 BG Intended Use The EnviroLogix QuickStix Kit for QuickScan Cry1A detects and quantifies Cry1Ab Bt11 or Mon810 event or Cry1A 105 in MON89034 event proteins at the levels typically expressed in genetically modified corn grain The sensitivity of these QuickStix Strips is 0 8 based on tests conducted with Mon810 corn i e one kernel in 125 For Bt detection in corn and cotton plant tissues and individual seeds please use QuickStix Cat AS 003 CRLS or CTLS How the Test Works In order to detect the Cry1A proteins the sample must first be ground and extracted in water to solubi2. ested Extrapolation as to the condition of the originating lot from which the working sample was derived should be based on sound sampling procedures and statistical calculations which address random QuickStix Kit for QuickScan Cry1A Bulk Grain Page 3 of 4 Rev 02 01 13 After 30 seconds add QuickStix to cup Strip must develop a Control Line to be valid cut where indicated and read in QuickScan System sampling effects non random seed lot sampling effects and assay system uncertainty A negative result obtained when properly testing the working sample does not necessarily mean the originating lot is entirely negative for the analyte or protein in question Protect all components from hot or cold extremes of temperature when not in use Do not leave in direct sunlight or in a vehicle QuickStix Kit for QuickScan Cry1A Bulk Grain Page 4 of 4 Rev 02 01 13 For Technical Support Contact Us At EnviroLogix 500 Riverside Industrial Parkway Portland ME 04103 1486 USA Tel 207 797 0300 Toll Free 866 408 4597 Fax 207 797 7533 e mail info envirologix com website www envirologix com LIMITED WARRANTY EnviroLogix Inc EnviroLogix warrants the products sold hereunder the Products against defects in materials and workmanship when used in accordance with the applicable instructions for a period not to extend beyond a product s printed expirati
3. f samples acceptable quality level and to determine the probability of accepting lots with given concentration levels It also plots the probabilities in graph form for easy interpretation Specific data can be saved for documentation and future analyses Transfer 20 mL extract to cup Either pour or pipette to the 20 mL mark outlined to demonstrate cup size and markings the sample cup to settle for 30 seconds so that any particles will settle at the bottom of the cup 9 To prevent cross contamination thoroughly clean blender parts and jars to remove dust and residue prior to preparation of each sample and use a new sample cup for each If pipetting always use a new pipette for each sample How to Run the QuickStix Strip Test 1 Allow refrigerated canisters to come to room temperature before opening Remove the QuickStix Strips to be used Avoid bending the strips Reseal the canister immediately 2 Place the strip into the sample cup provided being sure to insert the end indicated by the arrows on the protective tape The sample will travel up the strip 3 Allow the strip to develop for 5 minutes before making final assay interpretations 4 Immediately cut off and discard the bottom section of the strip covered by the arrow tape and place in QuickScan Reader Strips must be read while still wet NOTE Use extreme caution to prevent sample to sample cross contamination with grain fluids o
4. lize the protein Each QuickStix Strip has an absorbent pad at each end The protective tape with the arrow indicates the end of the strip to insert into the sample cup The sample will travel up the membrane strip and be absorbed into the larger pad at the top of the strip The portion of the strip between the protective tape and the absorbent pad at the top of the strip is used to interpret the reactions as described under Interpreting the Results Results are scanned and interpreted quantitatively with the EnviroLogix QuickScan System Please avoid bending the strips Sample Preparation 1 Collect a composite sample according to USDA GIPSA instructions found in the reference documents listed in the margin on Page 2 2 Determine the average weight of the grain from the lot to be tested Count and weigh 100 kernels seeds then divide by 100 3 Calculate the sub sample weight g needed for testing number of seeds X average seed weight A sub sample size of at least 100 grams is required to create enough extract for the test Weigh out the sub sample 4 Calculate water volume needed for sample preparation The Common Extraction method calls for a water volume to sample weight ratio of 1 5 to 1 Example Calculation using a 400 kernel sub sample with an average kernel weight of 0 3g 0 3g x 400 120g x 1 5mL 180 mL water for extraction 5 Weigh out the sub sample and grind using the Auto Drip setting on the Bunn grinde
5. omer the warranty it received if any from the maker thereof of such non EnviroLogix made products or components This warranty also does not apply to Products to which changes or modifications have been made or attempted by persons other than pursuant to written authorization by EnviroLogix THIS WARRANTY IS EXCLUSIVE The sole and exclusive obligation of EnviroLogix shall be to repair or replace the defective Products in the manner and for the period provided above EnviroLogix shall not have any other obligation with respect to the Products or any part thereof whether based on contract tort strict liability or otherwise Under no circumstances whether based on this Limited Warranty or otherwise shall EnviroLogix be liable for incidental special or consequential damages This Limited Warranty states the entire obligation of EnviroLogix with respect to the Products If any part of this Limited Warranty is determined to be void or illegal the remainder shall remain in full force and effect EnviroLogix the EnviroLogix logo QuickComb QuickStix QuickComb and Common Extraction are trademarks of EnviroLogix Inc EnviroLogix 2013
6. on date If the Products do not conform to this Limited Warranty and the customer notifies EnviroLogix in writing of such defects during the warranty period including an offer by the customer to return the Products to EnviroLogix for evaluation EnviroLogix will repair or replace at its option any product or part thereof that proves defective in materials or workmanship within the warranty period ENVIROLOGIX MAKES NO OTHER WARRANTIES EXPRESS OR IMPLIED INCLUDING BUT NOT LIMITED TO ANY IMPLIED WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE The warranty provided herein and the data specifications and descriptions of EnviroLogix products appearing in EnviroLogix published catalogues and product literature are EnviroLogix sole representations concerning the Products and warranty No other statements or representations written or oral by EnviroLogix employees agents or representatives except written statements signed by a duly authorized officer of EnviroLogix Inc are authorized they should not be relied upon by the customer and are not a part of the contract of sale or of this warranty EnviroLogix does not warrant against damages or defects arising in shipping or handling or out of accident or improper or abnormal use of the Products against defects in products or components not manufactured by EnviroLogix or against damages resulting from such non EnviroLogix made products or components EnviroLogix passes on to cust
7. r or equivalent The sample should be the consistency of coffee grounds 60 70 of the sample should pass through a 20 mesh sieve 6 Place sub sample into an appropriately sized jar or zip type plastic bag and add the volume of tap water calculated in Step 4 7 Cap the jar or zip plastic bag and shake vigorously for 30 seconds then allow sample to settle for another 30 seconds 8 Transfer 20 mL of the liquid portion from above the settled sample into the sample cup Pour extract into cup to the 20 mL line or use a fresh pipette from the kit to transfer extract until the 20 mL line is reached Important Avoid transferring particles as much as possible and after transfer allow the liquid in QuickStix Kit for QuickScan Cry1A Bulk Grain Page 2 of 4 Rev 02 01 13 USDA References http archive gipsa usda gov reference library handbooks grain insp grbook1 bk1 pdf USDA Grain Inspection Handbook Book 1 Grain Sampling http archive gipsa usda gov biotech sample2 htm Guidance document entitled Sampling for the Detection of Biotech Grains http archive gipsa usda gov biotech sample1 htm Practical Application of Sampling for the Detection of Biotech Grains http archive gipsa usda gov biotech samplingplan1 xls This website provides a simple to use Sample Planner 29K Excel Spreadsheet The planner allows you to enter different assumptions in terms of sample size number o
8. r disposables Interpreting the Results Development of the Control Line within 5 minutes indicates that the strip has functioned properly Any strip that does not develop a Control Line should be discarded and the sample re tested using another strip Results are scanned and interpreted quantitatively with the QuickScan System Place QuickStix Strip into the carrier slide in and press Read Test on the screen QuickScan will return a result as GMO or lt LOD less than the Limit of Detection Please consult the QuickScan User Manual for details Kit Storage QuickStix can be stored at room temperature or refrigerated for a longer shelf life Note the shelf life on the kit box for each storage temperature The kit may be used in field applications however prolonged exposure to high temperatures may adversely affect the test results Do not open the desiccated canister until ready to use the test strips Precautions and Notes This kit is designed to give a quantitative result using the QuickScan System and is not intended to be visually interpreted As with all tests it is recommended that results be confirmed by an alternate method when necessary The assay has been optimized to be used with the protocol provided in the kit Deviation from this protocol may invalidate the results of the test The results generated through the proper use of this kit reflect the condition of the working sample directly t
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