YMC-Triart C18, C8, Phenyl, PFP
Contents
1. about 50 mM or less buffer salts additives could be replaced directly with about 60 acetonitrile aqueous solution General proposals O Flushing with 100 water after using the column around the pH limit may shorten column lifetime Flush the column with water organic solution described above such as 60 acetonitrile aqueous solution O Once macromolecules like proteins or polysaccharides adsorbed onto the gel they are hardly removed even if solvents with high eluting ability are used In order to avoid contamination of the column by them conduct sample pretreatment carefully prior to introducing to the column or use a guard column YMC Co Ltd YMC Karasuma Gojo Bldg 284 Daigo cho Karasuma Nishiiru Gojo dori Shimogyo ku Kyoto 600 8106 Japan Tel 81 75 342 4515 Fax 81 75 342 4550 www ymc co jp sales ymc co jp YMC America Inc YMC Europe GmbH YMC India Pvt Ltd YMC Korea Co Ltd www ymcamerica com www ymc europe com www ymcindia com www ymckorea com YMC Shanghai Rep Office YMC Singapore Tradelinks Pte Ltd YMC Taiwan Co Ltd www ymcchina com www ymc sg www ymctaiwan com UM130111AE2. YMC HPLC COLUMN USER S MANUAL YMC Tnart C18 C8 Phenyl PFP HPLC 5 um 3 um UHPLC 1 9 um Column Care and Use Instructions 1 Introduction Thank you for purchasing a YMC high performance ultra high performance liquid chromatography HPLC UHPLC column YMC Triart is a multipurpose reversed phase column utilizing hybrid silica gel The column is designed to work under various analytical conditions and deliver exceptional selectivity and stability YMC Triart columns are manufactured under highly controlled conditions and must pass a series of strict tests before being accepted for shipment Please refer to the column inspection report In order to ensure optimal performance and durability of the column please read these instructions carefully before using this column 2 Specifications 5 Particle Pore Usable temperature range Packing aye E Functional group End Usable pH aaa material capped range eguiar use Upper limit Lmg nm recommended Peel Triart C18 C18 Yes o 10 12 2400c PH107 FOC Triart C8 C8 Yes pH 70 12 0 C 1 9 3 5 12 Triart Phenyl Phenyl Yes 10 10 200 40 C 50 C Triart PFP Pentafluorophenyl No 108 200 40 C 50 C 3 Recommendations for column connections detector settings and data processing considerations O The PT or WT at the end of the product code indicates the style of column endfittings see below for details Consideration of connector and endf
3. ctor Make sure not to have void in connection gt Use a detector equipped with low volume flow cell designed for narrow bore column gt Use an injector for narrow bore column and low volume sample loop O A sampling rate and a detector response time constant should be optimized to acquire more than 10 data points across a peak For UHPLC analysis using 1 9 um columns we recommend a sampling rate of about 10 points per second or higher and a detector response of 0 1 seconds or faster to detect earliest eluting sharp peak properly 4 Shipping solvent 100 Acetonitrile Replace with this solvent for storage When replacing a mobile phase containing buffer or salts take extra care to prevent precipitation of salt UM130111AE YMC HPLC COLUMN USER S MANUAL 5 Precautions for use O The correct direction of solvent flow is indicated by an arrow on the column identification label O Do not disconnect a column from LC system before the pressure drops to zero O Column pressure limit and recommended flow rate is as follows Particle size Pressure limit Column I D and recommended flow rate Mobile phase condition acetonitilefaqueous solution Column length of 150 mm and less 20 MPa 2 0 mml D 0 2 mL min 5 um 3 um Column length of 250 mm 25 MPa 3 0 mml D 0 4 mL min Inner diameter of 10 mm and larger 10MPa 4 6 mml D 1 0 mL min 2 0 mml D 0 2 0 8 mL min 12pm 100 MPa 3 0 mml D 0 4 1 6 mL min Avoi
4. d using a column repeatedly near the pressure limit or abrupt change in pressure to prevent from shortening column life Adjust flow rate appropriately as pressure changes depending on column length temperature types of organic solvent etc O Aqueous or non aqueous solvents can be used as a mobile phase Repetitive replacement among solvents with large difference in polarities may result in degradation of column performance In general acetonitrile methanol and tetrahydrofuran THF are recommended for regular use Please also see above recommendation under alkaline mobile phase conditions When using THF as a mobile phase mind the solvent resistance of your system or tubing Especially PEEK parts are not suitable for use with THF O Recommendations of pH and temperature for column use are shown in the specifications table in section 2 Also please note the following Column lifetime varies depending on conditions of use such as pH temperature and mobile phase composition In general using under higher temperature higher concentration of buffer salts additives and or lower concentration of organic solvents may result in shorter column lifetime When using the column under alkaline conditions for a long term a column should be used with a low concentration about 1 to 10 mM of organic buffer like triethylamine glycine etc at a lower temperature less than 30 Q The mobile phase containing methanol would give longer column lifetime unde
5. ittings Tubing and connector Column Mae in Port depth Style of endfittings in zl PT ca 2 mm 0 09 inch Parker style UPLC compatible WT ca 3 mm 0 13 inch Waters style A Port depth UPLC is a registered trademark of Waters Corporation O Tubing must have flat ends and must bottom out in the column endfitting Tubing must be connected to the column correctly to avoid creating a void between column frit and tubing which can cause a leak and or result in poor column performance e g peak tailing loss of theoretical plate number O 1 9 um column for UHPLC gives high operating pressure compared to conventional 5 um 3 um columns Pay attention to the usable maximum pressure of tubing and LC system Generally UHPLC system which has 60 MPa 8 700 psi or higher pressure tolerance is recommended for use with 1 9 um column We have a connector with re adjustable ferrule which provides flawless sealing at 137 MPa 20 000 psi Please inquire us for the detail O Extra column volume has great impact on band spreading In order to minimize the influence of band spreading on chromatographic performance especially when using columns in 2 0 mmI D and smaller LC system should be optimized as follows gt The shortest possible length of tubing with narrow inner diameters tubing less than 0 15 mm 0 006 inch I D is recommended should be used for connection from the injector to the column and from the column to the dete
6. r alkaline condition compared to other organic solvents O0 Make sure of miscibility among the organic solvents and take care to prevent the precipitation of buffer salts additives to avoid over pressuring the column O When possible the sample should be dissolved in a solvent in the same composition as the initial mobile phase Using the stronger solvent than the initial mobile phase for sample dissolution may result in distortion of peak symmetry and degradation of resolution O In order to prevent exposure of the column to excessive pressure the mobile phase and sample solution should be filtered through a 0 2 um membrane or smaller to remove particulates 6 Column cleaning general method After using mobile phase not containing buffer salts additives O Flush the column with solution containing higher ratio of organic solvent for washing out the compounds that have a great capacity to retain in the column O0 Usable concentration of organic solvent is up to 100 A cleaning solution containing THF may be effective when removing highly hydrophobic lipid soluble substances adsorbed onto the gel After using mobile phase containing buffer salts additives O Firstly replace with water organic solution that do not contain buffer salts additives A ratio of water to organic solvent should be set at the same proportions as a mobile phase Then flush the column in accordance with the method mentioned above O0 The mobile phase containing
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