Resolving Communication Problems
Contents
1. NOTE If it is performed properly the three status lights will be lit indicating the firmware has been cleared 6 Start the computer If Data Collection software does not launch automatically manually launch tt 7 A dialog box will appear indicating firmware is being reloaded NOTE If firmware does not automatically load then the preferences may be corrupted or set improperly for a detailed tutorial regarding setting preferences please refer to the appropriate Preferences document for your operating system at 310 Preferences Preferences Module for Macintosh computer 310 Preferences Module for Windows NT 2000 and XP OS 8 From the Window menu select Manual Control which opens a new window 9 From the Function drop down list select Syringe Home and click Execute This will bring the syringe pump up to its homed position NOTE After homing the syringe pump use the Syringe Down command to bring the syringe pump back on top of the syringe plunger 10 Select Autosampler Home X Y and click Execute 11 Select Autosampler Home Z and click Execute If the instrument is able to complete all these steps communication is re established lf the instrument is not able to communicate after all these steps please contact AB Technical Support for further troubleshooting Page 5 of 9 Resolving Communication Problems Module A 488 me COM1 Port Contacting AB Technical Support By Telephone 1 800 82. 31 6844 By Internet http www appliedbiosystems com support Then click on Frequently Asked Questions and then the Ask a Question tab By E mail ABTechnicalsupport appliedbiosystems com Control Data Port Page 6 of 9 Resolving Communication Problems Module BS Be m G3 G4 Computer Serial Port Manually Launching Data Collection Software ei Double click on the shortcuts on the FJ computer desktop eee Alternatively navigate via the Apple aag SBI Pram 310 Menu Start Menu Am Pria FF a Dalian tian Collection bat On the PC On the Macintosh computer Modem Port Page 7 of 9 Resolving Communication Problems Module Syringe Pump at Home Position NOTE After executing the SYRINGE HOME command the syringe pump must be brought back down on top of the plunger Ensure that the pump is close to but not compressing the syringe plunger If the syringe pump is not returned to this position prior to a run the instrument will generate a Leak Detected error Three Status Lights For Research Use Only Not for use in diagnostic procedures Notice to Purchaser License Disclaimer Purchase of this software product alone does not imply any license under any process instrument or other apparatus system composition reagent or kit rights under patent claims owned or otherwise controlled by Applera Corporation either expressly or by estoppel Page 8 of 9 Resolving Communication Pro
3. Applied Biosystems To navigate within this document use the dm OF oO oS keys ABI PRISM 310 Genetic Analyzer Resolving Communication Problems BEFORE PERFORMING ANY TROUBLESHOOTING WORK ON YOUR ABI PRISM 310 GENETIC ANALYZER PLEASE READ THE INSTRUMENT USER S MANUAL FOR SAFETY AND WARRANTY INFORMATION AND FURTHER DETAILS ON USE OF THE SYSTEM NOTE Text in this fashion indicates a link to a picture or another section of this another document Please contact AB Technical Support if you have any questions regarding this procedure About Communication Problems The ABI PRISM 310 Genetic Analyzer system consists of hardware controlled via software installed on either a PC or Macintosh computer Proper communication between the instrument and the computer must exist to e Send commands from the computer to the instrument e Receive data from the instrument to the computer Communication can be lost if e The system is powered up incorrectly e Communication related settings are e Power failure Recovery trom power loss changed e Instrument is shut down during a run e Communication cables are loose e Data Collection Computer is shut down damaged or plugged incorrectly during a run e Communication ports plugs are e Local area network compromised interference interruption e Firmware is corrupted or compromised Before You Begin Since communication loss results from many variables the following approach is a comprehensive albe
4. It is possible for actual power failures to cause severe damage to the ABI PRISM 310 Genetic Analyzer Contact AB Technical Support immediately If any uncharacteristic function appears Running the instrument while the computer workstation is attached to an active network can cause various communication problems including e Loss of communication e Loss of data Compromised data e Termination of runs prematurely e Errors that pause the system mid run To troubleshoot recurring problems of this nature disconnect the system from the network entirely while runs occur Connect to the network only when runs have completed and data is to be transferred Page 3 of 9 Resolving Communication Problems Module Applied Biosystems Step Possible Cause Action 5 Additionally take the following suggestions regarding continued general computer set up into consideration e The computer workstation should be within a firewall protected network rather than having firewall software installed locally Anti virus software should not be set to run on an automated schedule To minimize possible interference anti virus scans should be started manually when a run is not occurring Do not set the computer workstation CPU to sleep or hibernate mode Do perform hard disk defragmentation optimization at least once a month 6 Communication ports On Macintosh systems or plugs are e lf your computer utilizes a Keyspan Serial Card c
5. blems Module Applied Biosystems Applied Biosystems ABI PRISM and GeneScan are registered trademarks and AB Design and Applera are trademarks of Applera Corporation or its subsidiaries in the US and or certain other countries All other trademarks are the sole property of their respective owners PN 4375359 Rev A Stock No 106GU28 01 Page 9 of 9 Resolving Communication Problems Module
6. e On Macintosh computer Modem port e On Mac G3 G4 computer Serial port 1 e OnPC COM1 port 4 Turn on the instrument then turn on the computer 5 If Data Collection software does not launch automatically manually launch it 6 From the Window menu select Manual Control which opens a new window 7 From the Function drop down list select Buffer Valve Close then click Execute 8 If you hear see the buffer valve pin close communication is re established If you do not hear see the buffer valve pin close continue with step 3 NOTE If the cable is compromised these parts can be purchased through AB Mac cable AB p n 4818551 or PC cable AB p n 4316489 or your local retailer 1 If Data Collection software does not launch automatically manually launch it 2 From the Window menu select Preferences then select General Settings 3 In the Communication Port window On the Macintosh computer Click on the Modem icon then click OK ane NOTE On certain Macintosh Operating Systems the icon will not highlight when the Modem icon is depressed but the selection is still being made Qn the PC From the drop down list select COM1 then click OK Page 2 of 9 Resolving Communication Problems Module Applied Biosystems Step PossibleCause Atom OoOO o O 3 continued Power failure Recovery from power loss OR Instrument is shut down during a run Local area ne
7. it lengthy procedure to re establish communication If problems persist after completing these steps please contact AB Technical Support for assistance Resolving Communication Problems Step Possible Cause System is powered up 1 Shut down the computer then shut down the instrument incorrectly 2 Turn on the ABI PRISM 310 Genetic Analyzer Wait until the status lights are on OR 3 Start the computer If Data Collection software does not launch automatically manually launch tt Data Collection 4 From the Window menu select Manual Control which software or the opens a new window computer is shut down 5 From the Function drop down list select Buffer Valve during a run Close then click Execute 6 If you hear see the buffer valve pin close communication is re established If you do not hear see the buffer valve pin close continue with step 2 Page 1 of 9 Resolving Communication Problems Module Step Possible Cause Communication cables are loose damaged or plugged incorrectly Communication related settings are changed Applied Biosystems Action 1 Shut down the computer then shut down the instrument 2 Carefully remove the communication cable between the instrument and the computer Inspect the ports and plugs for dust broken or bent pins frayed wiring etc 3 Carefully reinsert attach the communication cable to the following locations e Onthe 310 Genetic Analyzer oo Control Data port
8. ompromised Adapter you may need to reinstall reconfigure the PortConnector and or KeySX Manager software Refer to the appropriate user documentation e lf you are using a Mac G3 G4 computer of the blue white or grey white configuration you may need to reinstall the driver for the gPort g4Port universal serial adapter Refer to the appropriate user documentation On PC systems 1 If Data Collection software does not launch automatically manually launch it 2 From the Help menu select Communication Diagnostics which opens a diagnostics window 3 View the Send Reply table e Alarge number of retries indicates possible bad cable routing broken contact pins or compromised cable e A long duration for replies Send Reply may indicate a computer serial driver problem 7 Firmware is corrupted Perform a cold boot reset or compromised 1 Ensure that both the computer and the ABI PRISM 310 Genetic Analyzer are turned off If not shut down the computer first then shut down the instrument 2 Open the instrument doors 3 Press and hold the Tray button down with your right hand 4 Keeping the Tray button depressed reach to the left rear of the instrument and turn on the ABI PRISM 310 Genetic Analyzer 5 After turning the instrument on release the Tray button Page 4 of 9 Resolving Communication Problems Module Step Possible Cause 7 continued Further troubleshooting necessary Applied Biosystems
9. twork interference interruption For both systems 4 Restart the computer 5 If Data Collection software does not launch automatically manually launch it 6 From the Window menu select Manual Control which opens a new window 7 From the Function drop down list select Buffer Valve Close then click Execute 8 If you hear see the buffer valve pin close communication is re established If you do not hear see the buffer valve pin close continue with step 4 1 If the instrument suffers an actual power loss or the instrument is accidentally powered down during a run the instrument may perceive it as a power failure 2 On Macintosh systems only the Data Collection software may generate a recovery file that interrupts normal operation sometimes generating a Recovering from Power Failure error 3 From the Macintosh Finder window navigate to the ABI PRISM 310 Data Collection folder and look for a file called Recovery Alternatively perform a search for Recovery 4 If there is a Recovery file drag it to the trash and empty the trash 5 Open the Data Collection software 6 From the Window menu select Manual Control which opens a new window 7 From the Function drop down list select Buffer Valve Close then click Execute 8 If you hear see the buffer valve pin close communication is re established If you do not hear see the buffer valve pin close continue with step 5 NOTE
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