9110 Instruction Manual
Contents
1. 0ce Cat No 9000 507 Silicone tubing ID 2 00mm1meter running length 0eee Cat No 9000 509 Silicone tubing ID 3 00mm1meter running length 008 Cat No 9000 508 Tube clamp for continuous 1 0mm tube set of 2 ceeeee Cat No 9000 601 Tube clamp for continuous 2 0mm tube set of 2 cceeeeees Cat No 9000 602 Tube clamp for continuous 3 0mm tube set of 2 ceeeeees Cat No 9000 603 Unoverse 9100 wall mounting bracket Stand cceecceeseeeeeeeees Cat No 9100 001 FOO sts sie eee saeco eee E EE eee seee sence neseetsanieen Cat No 6013 8 way dispense comb iss sccssasecidcacasseasaserstscneieanasadarsseasssaanaraaeesiauatiancanaaaes Cat No 3022 Yellow tip 1 200qyl een enn ee eee eee Cat No 3530 TID NOOSE SUDOM esee Aai Cat No 9100 3003 9100 001 9000 601 ej 9000 602 Pj 4 9000 603 47 9100 on mounting bracket available as accessory 9000 502 Page 102. APPLICATION 7 Choosing the correct working range There are 4 working ranges to choose from These working ranges correspond to tube diameters The decimal point is shifted corresponding with the working range R1 ID 0 5mm for lt 9ml R2 ID 1mm for lt 25ml R3 ID 2mm for lt 90ml R4 ID O3mm for lt 190ml max display 9 000ml max display 25 000mI max display 90 000ml max display 190 0ml The single ul digit can not be entered in R2 amp R3 Working with low volume dispenses with large diameter tube will reduce both precision and accuracy of dispenses Using the U D keys scroll to the screen displaying Range Enter Press Enter Using the U D keys choose the range corresponding to your tube diameter and press Enter The display will show the last volume you worked with at that particular range 8 Gravimetric pump calibration procedure Pump flow performance is subject of many factors among them the condition of the peristaltic tube and the viscosity of the reagent It is therefore important that you calibrate the pump before it is commissioned using your reagent and at periodic intervals Carry out the calibration procedure using a volume nearest to the volume yiou are most likely to dispense You can choose any volume 1 Carry out 5 identical dispenses into a vessel and establish the gravimetric volume dispensed in each operation Calculate the mean dispensed volume Using the U D keys scroll to disp
3. un dispensed volume it will display the amount you dispensed so far Press GO and the unit will dispense the remainder of the volume you originally wished to dispense If you prefer the unit to forget the remaining volume press the ESCAPE key after you pressed the STOP key The unit will display the last volume you entered 12 Changing the pumps direction The pump controller can be used for dispensing or aspirating reagents The slide switch on the left side of the unit sets the direction that the pumps rotor rotates You can not change direction when the pump is in dispense mode You can however press the STOP key change direction by moving the slide switch and continue by pressing the GO key allowing the pump to dispense or aspirate the remainder of the entered volume in the reversed direction Page 6 13 Interval Dispense You can set the unit to dispense any volume for a number of times in predefined intervals This feature is useful in repeatable operations either in manual mode or when the dispense head is held by a robotic arm Intervals can be set between 0 5 sec to 100 minutes To set repeat dispensing 1 Using the U D keys scroll to the screen saying Interval Enter 2 Press Enter and you are asked to enter the Delay in minutes and seconds Any number up to 99 59 will be accepted whereby any number bigger than 59 for the seconds will be rejected Using the numeric keys enter the numbers of minutes and se
4. Box contains The following Boxer 9100 pump Controller ccccccccceccceeeeseeeceeesaeeeseeeseeeeaeeeaes Cat No 9110 000 PSU output 24V 1A with universal plug adapters c c6cce Cat No 9200 004 BOXER 91 00 Set tube clips NO 1 a n aannnannnnnnnnnnnnnnsnnnnnrnsnrnsnrnsnreenrnerrnenrrenrnerrnenne Cat No 9000 601 Set tube clips NO 2 0 cccccecccsececseeecceeeeceeeeceaeeseaeesseeeeseeeessneessees Cat No 9000 602 Set tube clips NO 3 0 cecccccececeeeeeceeeeceeeeseecesseeesseeeseeeeseeeessaees Cat No 9000 603 1 ID 2 0mm Silicone tube 1 Metre cc ceececeeeeeeeeeeeeeeeeeeeees Cat No 9000 509 2 In line connector tube to yellow tip cccceeeceeeeseeeeeeeeeeeeeeeeeeeeeeeees Cat No 3923 3 Tip holder SUPP OM iis cx csccceocecceeecccverdsgelcethavctenesdcesebensesietendeseenes Cat No 9100 3003 Electronically controlled 4 Yellow Dispense Tip 1 100U LW1035 n Cat No 3530 dispensing pump This instruction manual cceeeeccceceeeeeeeeeeeeeeeeeeeeeeseeeeeeeesannesess Cat No 9110 003 Accessories amp spares Silicone Tubing ID 1 0mm complete with connectors 008 Cat No 9000 504 Silicone Tubing ID 2 0mm complete with connectors 06 Cat No 9000 505 Silicone Tubing ID 3 0mm complete with connectors 068 Cat No 9000 510 Santoprene Tubing ID 1 0mm complete with connectors Cat No 9000 501 Santoprene Tubing ID 2 0mm
5. PVC tube 83ml min for the 9110 010 which has max rotor speed of 520rpm 3 The peristaltic tube The supplied peristaltic tube is made from Silicone Please make sure the reagents you intend to use are compatible with these material 3 A Clamping the peristaltic tube The pump controller is delivered with a ID 2 0mm Silicone tube Move the lever up to open the clamp and remove the clamp from the pump Insert the tube into the two tube holders on each side of the tube clamp and make sure the tube follows tangent and the arc of the clamp Push the tube clamp back onto the pump and lower the lever the tube is now occluded by the rollers 3 B Releasing the peristaltic tube when you have finished Whenever the pump is not in use for long periods of time it is important to release the pressure off the tube This will reduce tube deformation and ensure that accuracy precision is maximised Release the pressure by lifting the pressure lever 3 C Replacing the tube Tube need replacing when worn out or wherever you intend to change the dispense volume range in which you work for smaller or larger dispense volumes Lift the pressure lever and replace the tube making sure that you have installed the tube centrally and that the tube is fully engaged by the tube guides Page 2 Pressure lever 4 The choice of tubing The unit can work either with fixed length tubing 75mm long within the tube clam
6. complete with connectors Cat No 9000 502 Santoprene Tubing ID 3 0mm complete with connectors Cat No 9000 518 Santoprene tubing ID 1 00mm1meter running lengfth 0 Cat No 9000 521 Santoprene tubing ID 2 00mm1meter running lengfth 0 Cat No 9000 506 Santoprene tubing ID 3 00mm1meter running length 0 Cat No 9000 515 Silicone tubing ID 1 00mm1meter running length ceee Cat No 9000 507 Silicone tubing ID 2 00mm1meter running length c ceee Cat No 9000 509 Silicone tubing ID 3 00mm1meter running length ceee Cat No 9000 508 Bag of 25 Yellow Dispense Tips1 100ul LW1035 eee Cat No 9100 005 Spare dispense set consisting of above items 1 4 ee Cat No 9100 006 Unoverse 9100 wall mounting bracket Stand cccceeeeeeeeeeeees Cat No 9100 001 FOOT ONNO 9 ence en ee ee ne ee ne eee ne ene oe ace Cat No 6013 Product Specification Mains Voltage cccccccseeeeeseeeeeeeeeeeeeeeeeeeseeeeeeseeeeeeas 100V 240V 0 3A MAX 47 63Hz Power SUDDIY Unit OUTPUT wcccceccccecc ce cnescnaccedanccecnersestedacredencesommasteetdeandeceates 24VDC 1A Environmental operating temp ccccccccceecceeeeseeeeeeeeseeeseeeseeeeaeeeaes 10 C to 40 C Environmental Storage temp ccccccccseecceeeeeeeeeeeeeeseeeeeeeeeseeeseeeesaes 4 C to 40 C Environmental operating RH ccccss
7. conds The number 00 00 represents 7 2 second Press Enter to confirm 3 Repeats screen asks you to enter the number of repeats from 1 to 98 Entering 99 repeats will result in continuous number of dispenses which can only be stopped by pressing the STOP button Using the numeric keys enter the number of repeats Press Enter to confirm 4 You can exit the interval mode by pressing the ESCAPE key 14 Pump speed adjustment To adjust the dispense speed scroll the menu using the U D arrows until the Speed Enter Is displayed Press Enter and the screen will display Speed with 8 bars on bottom line Use the U D keys to increase or decrease number of bars More bars represent higher speed Press the Enter key to enter the desired speed into the instruments memory 15 Continuous dispense mode To run the pump continuously scroll the menu using the U D arrows until Constant Enter Is displayed Press Enter and the screen will display Constant Stop Go Press the GO button to start the pump and the STOP button to stop it at any point Press the ESCAPE key to leave the continuous dispense mode Please note running the peristaltic tubes dry over along period will shorten their life Page 7 16 Remote control operation The instrument is fitted with a stereo type 2 5mm socket for remote control Through this socket you can plug in the foot pedal which will provide the unit with a GO signal The un
8. e In these circumstances the required volume input does not divide into stored values and therefore the calculator will round up or down to the nearest whole figure 5 Q Can leave the unit switched ON throughout the day A Yes you can The dispenser requires very little energy when the pump is not running so continuous power into the unit will not harm it 6 Q Can I dispense hot reagents using the dispenser A Yes the dispenser to works safely with media temperatures of up to 100 C Be aware that the internal electronics could be damaged by any ingress of fluids or steam vapours Page 9 19 Accessories amp spares Silicone Tubing ID 1 0mm complete with connectors 006 Cat No 9000 504 Silicone Tubing ID 2 0mm complete with connectors 08 Cat No 9000 505 Silicone Tubing ID 3 0mm complete with connectors 00 Cat No 9000 510 Santoprene Tubing ID 1 0mm complete with connectors Cat No 9000 501 Santoprene Tubing ID 2 0mm complete with connectors Cat No 9000 502 Santoprene Tubing ID 3 0mm complete with connectors Cat No 9000 518 Santoprene tubing ID 1 00mm1meter running length Cat No 9000 521 Santoprene tubing ID 2 00mm1meter running length Cat No 9000 506 Santoprene tubing ID 3 00mm1 meter running length Cat No 9000 515 Silicone tubing ID 1 00mm1meter running length
9. it can be controlled from a computer via a USB relay controller which will convert an signal from your computer to a closed circuit on the remote wire connection A USB relay controller is available from many electronic components suppliers Full control of the unit is then possible using the software supplied with such relay The stereo cable to the unit can be fitted with 3 core wire allowing the equipment to send a busy signal back to the controller 9100 9200 START Work State 3V 24V DC if g PC Busy dispensing Com 0V 17 Care amp maintenance The control unit is maintenance free The peristaltic tubes however require replacement as soon as excessive wear or a large variation in dispense volumes are noticed The operational life of the tubes is a function of the speed load and reagent being dispensed Avoid running the tubes dry for longer than a few minutes Check the peristaltic tubes weekly for signs of excessive wear and replace if required Pump tubes which remain clamped in the pump will deform with time Therefore rotate the tube clip at the top of the pump to open position in order to relieve the pressure whenever the pump is not being used for long periods or overnight Page 8 18 Exclusion If the equipment is used in a manner not specified by the manufacturer the protection provided by the equipment might be impaired This instrument is NOT suitable for use in explosion hazard envi
10. lay saying Calibrate Enter Press Enter The display shows C to indicate that you are in the calibration mode and next to it the range which you calibrate i e 1 to 4 CY 6 Using the U D keys correct the volume to your calculated mean volume T Press Enter and the display shows the volume you intended to dispense The unit is now calibrated with a particular tube within your chosen working range Cee ai Volumes in R2 amp R3 will increment by steps of 10ul The unit will store the calibration you carried out for each individual range Page 5 9 Prime the tube To prime the tube direct the dispense tube towards the reservoir and hold down the GO key As soon as the tube is primed release the key and the pump will stop 10 Entering the dispense volume Press 1 to 0 to enter your required volume Note that the digits move from right to left with the decimal point staying stationary The unit will always display on the top line the range in which you are working To correct an error entry press the back space key Press GO to start dispensing The unit will remember the volume only for this single dispense Following this dispense the unit will revert to the previously displayed volume In order to enter the typed volume into the unit s memory press the Enter button after entering the volume 11 The STOP button The STOP button will stop the dispense immediately The unit remembers the remaining
11. p or with continuous tubing which in effect does not have any length restriction In both cases the correct tube clips have to be installed in the clamp For the continuous tubing there are three sizes of tube clips 1 Marked 1 for ID1 0mm tube 2 Marked 2 for ID 2 0mm tube oe Marked 3 for ID 3 0mm tube Clip No 2 is used for the fixed length tubing U D keys e Scroll menu e Choose range e Increase or decrease value when in calibration mode Page 1 Page 3 6 Preparing the pump for use Plug the supplied PSU into your power socket and the low voltage plug into the socket on the left side of the unit switch it on by pressing the ON button The green LED on the side and the display will illuminate indicating that the unit is operational The display will temporarily read BOXER 9100 and then the display will show the value it has in it s memory Note the direction of flow The pump can be reversed in flow direction using the slide switch so that you can aspirate any unused reagent back into the reservoir Remote pulse socket for connection to foot switch Accessory or pulse from a robotic processor Accepts 2 5mm mono or stereo plug Power socket for 24V DC 1A Pump direction slide switch Front Bottom side dispenswe Back Bottom side aspirate Power ON LED On Off switch Page 4 IMPORTANT CHOOSE THE RANGE THAT MATCHES THE ONE YOU ARE MOST LIKELY TO USE IN YOUR
12. ronments This equipment is not suitable and should not be used for medical applications 19 FAQ 1 Q Why does the pump stalls or runs slower than expected when new tubing is installed A A new tube is stiff when new so has to be shaped for some time before it gains it s elasticity Run the pump in continuous mode for a total of approx 5 minutes with the clamp lever half open to start with gradually moving the clamp lever to fully closed position 2 Q have entered a volume but nothing happens after pressing the GO button A The volume you entered is too small for the tube size you use The motor can not turn accurately a very small amount and the dispense is cancelled Use a smaller tube diameter for low volume dispenses 3 Q What is the life of the tube A The life of a peristaltic tube depends on many variants such as speed the reagent it is pumping and temperature In general you should expect approximately 800 hours operation from one Pharmed tube and approx 150 hours from a Silicone tube 4 Q Why does the displayed value sometime jump in double figures A This happens when dispensing small volumes and instrument in course calibration Following the calibration procedure the instrument s mathematical calculation divides the volume to be dispensed by the number of pulses received from the motor s encoder and stores this value If the volume per rotor rotation is relatively large the ratio of pulses to volume is coars
13. s Retain the packaging so it can be used for future shipping The plug in power supply unit is Switch Mode and automatically adjusts itself to the mains power supply characteristics It will work with any mains voltage supply from 100V to 240V The power supply unit is shipped with three alternative plug adaptors to fit European American and UK power outlets Select the correct adaptor for your needs and clip it into the power supply 2 General information on peristaltic pumps Peristaltic tube pumps are ideal for fluid transfer metering and dispensing In contrary to centrifugal and gear pumps peristaltic pumps handle fluids of various viscosities are self priming and can operate in either flow direction With no valves seals or packing to come in direct contact with the pumped fluid they are ideal for pumping high purity amp corrosive fluids and for contamination free dosing The principle of the peristaltic pump is based on a tube which is occluded by a series of rollers AS a general rule the higher the number of rollers and the smaller the tube diameter the lower are the flow rates but better is the accuracy and precision The 9100 controller is equipped as a standard with 3 roller system and 2 0mm ID tube This tube diameter together with maximum speed delivers a flow rate of 48ml min The highest dispense accuracy is achieved by using a semi rigid tube on the outlet of the pump We recommend thin wall tubing such as FEP or
14. scccssececssceceucecseeeseeecenseeeseuseseueesseesensesenees 90 Environmental storage RH cimsetiservedancicencsesirnclianiuactvastideedcdvaceatianilecinboussinwadavasss 95 It is the stated philosophy of Uno International Ltd to preserve the environment wherever possible Uno International Ltd will only use materials and production techniques that cause least environmental damage User Manual Uno International Ltd 20 Belsize Avenue London NW3 4AU UK The CE sign certifies that the instrument Tel 44 20 7794 4080 meets the requirement of the EEC FAX 44 20 7431 1426 directives and has been tested according Email uno boxerpumps com to the specified test methods ISS1 14 Chemical compatibility chart lade Chlorine wet dry Lead salts Plating solutions GooS S S S S S S fcr i l e Carbon tetrachloride Ethyl chloride Trichloroethylene Perchloroethylene Chlorobenzene Freon Lacquer Toluene Chloroform Gasoline unleaded Naphtha c Som Q O c a c O D O c Som O l Moderate effect Severe 1 A Unpacking Remove the packing materials unpack the pump controller and the power supply unit Make sure that you have got all the components as specified in Box Contains last page of this manual Please contact your supplier if you notice any one of the components is missing or damaged Note Do not attempt to assemble a unit using damaged component
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