AmpliFire User Manual
Contents
1. AmpliFire Mas User Manual 222 Version 2 0 DNAble 8 well Reader October 2014 Amplifire TABLE OF CONTENTS as Ia a mmmmmmmmmmmmmmmmmmmmmmmmmmmiu 2 SI EEEE EENE EE E EENEN I EEEE EEEE 3 Component ldentification 0000 5 Sea ees 6 ONE hun a 6 Technical SUPPO mmmmmmmmmmmmrmrmrrr rr ri 6 Operation 7 IE YT TSS mi 9 Exporting Deleting Test Results 10 Exporting Test Results 10 Deleting Test Results 10 Am pl FI re performs nucleic acid amplification and detection in 15 minutes or less Eguipped with data storage touch screen interface USB keyboard port manufactured by Douglas Scientific Unauthorized use of any kind including without limitation to modifications reformatting or transfer of information other than the first user the Buyer is not permitted without the express written consent Any unauthorized use may void the instruments warranty All drawings and designs are and shall remain the property of Douglas Scientific No rights or obligations other than those expressly recited herein are to be implied and no license hereby granted 2 of 10 ENVIR LOGIX SAFETY Read and understand this User Manual before operating or performing maintenance Failure to do so could result in serious injury or damage to equipment General e Before starting AmpliFire each time o Ensure Sample Station Cover is in working order o Ensure instrument is2. for extended periods Charging Process o Follow instructions exactly to control charging and discharging current o Never charge batteries unattended Always monitor charging process to assure batteries are being charged properly Failure to do so may result in fire Charge battery with AmpliFire charger only Let battery cool down to ambient temperature before charging Only charge battery when in AmpliFire unit Never store or charge battery pack inside car in extreme temperatures extreme temperature could ignite fire o lf battery starts to balloon swell up smoke or gets hot discontinue charging process immediately This may cause battery to leak and reaction with air may cause chemicals to ignite Disconnect battery and observe in a safe place and observe for approximately 15 minutes O O O O o Charge battery every two months when not in use Operating Charging Temperature 32 F 1 13 F Discharge 32 F to 140 F o During discharge and handling of batteries do not exceed 60 F Battery Life o Batteries that lose 20 of their capacity must be removed from service and disposed of properly Discharge battery making sure output wires are insulated then wrap battery in a bag for disposal N OTI CE Disposal of battery must be in accordance with national regulations 4 of 10 ENVIR LOGIX COMPONENT IDENTIFICATION Description Description lithium ion rechargeable battery included DNAble Reade
3. in good operating condition e To shut down AmpliFire turn power switch to off Storage and Transport e Transport only by carrying handle Pack securely for long term transportation shipping paying particular attention to protecting the touchscreen e Store in a dry location 20 F 140 F 29 C 60 C e Disconnect battery if stored for over one month Before Starting Instrument e Do not operate instrument unless you have been trained to do so Do not operate instrument until you read and understand operating instructions Thoroughly familiarize yourself with AmpliFire and its controls e Always wear safety glasses and any other required safety equipment as required by your company s Personal Protective Equipment PPE policy e Never remove warnings displayed on instrument Replace any worn or damaged labels Contact EnviroLogix for replacement labels e Recognizing Safety Labels o Warning Read User Manual Before Operation Fig o Caution Burn Hazard Fig 2 Operating e Only authorized personnel should operate this instrument s Never operate an instrument with a compartment lid removed e Operation environment temperature is 32 F 120 F 0 C 49 C e The AmpliFire is not designed to support weight of a person Do not step on any part of the instrument e Process only materials identified in sample and assay protocols with AmpliFire General Maintenance EnviroLogix is responsible for instru
4. ct Code scan QR Code Cancel e Scan assay bar code with bar code camera 1 Hold bar code approx 6 from camera to scan Unit will beep once when scan is complete DNAble Reader User Manual 2 0 e Once scan is complete protocol name is displayed 3 Press Next 4 Note Press 1 to add another reaction and scan next bar code if desired Note Press Re scan 2 to rescan code if necessary 56 0 C I Reaction 1 Step 1 Click Scan button to scan OR Code 3 pp 5Min 001 Cancel Enter a Reaction Name by pressing gray box 1 and entering with popup keypad File name will start with this description Enter custom field name in Custom Field 1 and in Custom Field 2 2 if applicable Note To remove keypad touch anywhere outside keypad or press keypad button 3 on left of keypad e Press Next 4 6 0 C Reaction 1 Step 2 Enter a name for this p ath Reaction Name L 1 Step 3 Enter a custom field label Cust jeld 1 in Custom Field 1 and in erin creme hy lt Custom Field 2 if 2 applicable Custom Field 2 ju a 7 of 10 AmpliFire Enter Sample ID for the first well and e Open sample station cover 1 and identify custom fields if applicable insert tubes 2 into sample station 3 Press Next 1 to advance and enter e Close sample station cover information for subsequent wells 2 8 Bia a Na Notice Place AmpliFire on flat surface Alte
5. d reaction 1 1 2006 o 1 1 2006 3 26 08 PM j SMin LJ Att12006 12 41 52 PM 0 Att12006 12 20 20 PM o 41112008 12 17 29 PM 2 DNAble Reader User Manual 2 0 9 of 10 AmpliFire EXPORTING DELETING aj A Ea ie TEST RESULTS e Select desired reaction date 1 Exporting Test Results e Insert USB into either USB port 1 e Select reaction file 2 to delete e Press Delete Selected 3 1 1 2006 a January 2006 5 Li r w T F 5 C 17172006 3 26 08 PM 25 26 7 58 29 30 31 49112006 12 41 52 PM m 3 45 6 T 101 2006 12 20 20 PM i 6 9 10 11 12 13 14 1 49112008 12 17 29 PM 15 16 17 18 19 20 21 122 23 24 25 26 27 28 3 29 30 3112 3 4 Today 1 1 2006 e Select desired reaction date 1 elete Selector e Select reaction file 2 to export e Press Export Selected 3 1 1 2006 C 171 2006 3 26 08 PM 55 26 27 28 20 30 31 Si assin ee Delete 1 Reaction s L 1 2006 12 20 20 PM C 111 2006 12 17 29 PM 22 23 24 25 26 Lance bo 3031123 4 cancel OK Today 1 1 2006 Export Selected Delete Selected Export Selected e Press OK 1 Note Files will be exported to USB in CSV format e Press Back 2 to exit to main screen e Note Checking Remove from device TECHNICAL SUPPORT Da POA fo Call Toll free US 1 866 408 4597 ext 2 l or 207 797 0300 ext 2 www envirologix com Saturday January 21 2006 Export 1 Reactions y 3 Remove from d
6. evice cs Delete Selected Export Selected 10 of 10 i ENVIR LOGIX NOTES
7. ment repairs always contact EnviroLogix before performing any repairs or maintenance to AmpliFire e Do not operate faulty or damaged eguipment e Always follow EnviroLogix guidance regarding service and maintenance to AmpliFire e Never open covers that house electrical components Cleaning e Use caution if using toxic or flammable solvents to clean instrument e Always wear safety glasses and any other required safety equipment as required by your company s PPE policy DNAble Reader User Manual 2 0 i 3of 10 Am pliFfre Heat Do not touch heated surfaces Sample Station Chemical Follow all SDS chemical sheet recommendations Do not touch ingest or inhale samples Battery Failure to read and follow instructions may result in fire personal injury and damage to property if charged or used improperly Use only AmpliFire unit to charge battery Do not use a a NiMH or NiCd charger Failure to do so may a cause fire which may result in personal injury and property damage Keep Lithium lon Li ion battery pack away from children Li ion battery amp packs may explode and cause fire if misuse or defective Li ion batteries have a finite life cycle replace old battery with new one when it reaches its service life or when it is two year old whichever comes first Use caution to avoid puncture of cell Puncture of cells may cause a fire Never drop batteries Do not expose battery pack to direct sunlight heat
8. r User Manual 2 0 5 of 10 Am pihe SET UP e Turn on AmpliFire using On Off switch e Press Settings button 1 56 0 C Execute Reaction View Results Enter instrument name by pressing gray box 1 and entering data with popup keypad Note To exit keypad press anywhere outside keypad Enter date 2 using drop down arrow Enter time 3 using drop down arrow Enter time zone using drop down arrow Press Save 4 Press Back 5 to return to main menu Note Delete Data 6 will clear all reactions from device MAINTENANCE IMPORTANT Over moistening cotton swab may result in liquid entering instrument which may result in damage Weekly cleaning instruction e Swab all areas of sample station with a cotton swab moistened with a 10 bleach to water solution or 70 30 Isopropyl Alcohol solution e Swab all areas of sample station using a cotton swab moistened with water Note Instrument use in dirty dusty conditions may require more frequent cleaning TECHNICAL SUPPORT Call Toll free US 1 866 408 4597 ext 2 or 207 797 0300 ext 2 www envirologix com OPERATION e Turn on AmpliFire using On Off switch Note Unit will warm to 56 C e Prepare sample according to directions e Press Execute Reaction 1 56 0 C Execute Reaction View Results e Press Scan Product Code 1 56 0 C Reaction 1 Step 1 Click Scan button to Scan Produ
9. rnatively if no further sample Do not open lid move unit or remove identification is required press tubes during test Finish 2 to skip past wells 2 8 Press Back 3 to return to main menu Note If setting up more than one test click on Reaction 1 4 to get to start test menu Only first test will be allowed to run Sample Id Well One e Press OK 1 Custom Field 1 Custom Field 2 Reaction 1 Weil Seven 2 2 2 4 2 2 2 e Press Start 1 Reaction 1 Well One e Sample test will start to run Press Well Two a Cancel 1 to stop test Time 1 Well Three ei Remaining 2 will count down 2 WellFour Well Five nn 56 0 C I i Reaction 1 ls Well Six Protocol protocol 1 min 56 C Time R ining 0 1 Well Seven SIM ok gfe 2 Well One Wel Eight 1 Well Two Well Three Well Eight Well Four Well Five dy Well Six Protocol protocol 1 min 56 C Ti ining 0 Well Seven ime Remaining 0 15 VIEWING RESULTS e Press Close 1 or 2 to e Press View Results 1 add view multiple test results Reaction 1 Well One GFAM MCA Red Well Two al Execute Reaction Well Three Well Four View Results Well Five Well Six Reaction 5Min 001 5 min 56 C Well Seven Time Remaining Complete _ e Select desired reaction date 1 e Select reaction file 2 to view e Press View 3 to view selecte
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