VC-8T User`s Manual (Rev. 030626.1)
Contents
1. Burton J Warner Position President Warner Instruments A Harvard Apparatus Company VC 8M Manual Rev 050817 1 Declaration of Conformity CE MARKING LVD Application of Council Directive 73 23 EEC Standards To Which Conformity Is Declared Manufacturer s Name Manufacturer s Address Equipment Description Equipment Class Model Numbers EN61010 1 1993 Warner Instruments LLC 1125 Dixwell Avenue Hamden CT 06514 Tel 203 776 0664 Valve Controller Safety requirements for electrical equipment for measurement and laboratory use Class I VC 8 Controller I the undersigned hereby declare that the equipment specified above conforms to the above Directive s and Standard s Place Hamden Connecticut USA Signature Full Name Burton J Warner Position President Warner Instruments A Harvard Apparatus Company VC 8M Manual Rev 050817 1 14 WEEE RoHS Compliance Statement EU Directives WEEE and RoHS To Our Valued Customers Harvard Apparatus is committed to being a good corporate citizen As part of that commitment we strive to maintain an environmentally conscious manufacturing operation The European Union EU has enacted two Directives the first on product recycling Waste Electrical and Electronic Equipment WEEE and the second limiting the use of certain substances Restriction on the use of Hazardous Substances RoHS Over time these Directives will be implemented in th2. a NEMA 5 15P connector is standard Warner Instruments A Harvard Apparatus Company VC 8M Manual Rev 050817 1 9 A fuse holder contains a protective fuse in series with the high side brown or black wire of the mains The holder accepts 5 x 20 mm fuses of the type indicated INSTRUCTIONS FOR USE Flow Adjustment Flow rates can be adjusted by raising or lowering the reservoir holder as well as by adjusting the height of each reservoir within the holder The table below lists the approximate flow rates for a reservoir at the specified height with the supplied PTFE tubing Reservoir height Approximate flow rate 61 cm 24 in 14 ml min 30 cm 12 in 9 ml min 20 cm 8 in o ml min MAINTENANCE Cleaning Do not use alcohol aromatic hydrocarbons or chlorinated solvents for cleaning They may adversely react with the plastic materials used to manufacture the system If salt solution spills on the valve assembly it should be cleaned as soon as possible with a soft cloth dampened with a mild solution of detergent and water The exterior of the CONTROLLER may be cleaned periodically to remove dust grease and other contamination It is not necessary to clean the inside Use a soft cloth dampened with a mild solution of detergent and water Avoid abrasive cleaners Warner Instruments A Harvard Apparatus Company VC 8M Manual Rev 050817 1 10 APPENDIX Warranty The VC 8T Valve Control System is warranted to be free f
3. VC 8T Manual Rev 050817 1 Warner Instruments Perfusion Valve Controller Model VC 8T Warner Instruments 1125 Dixwell Avenue Hamden CT 06514 800 599 4203 203 776 0664 203 776 1278 fax CC VC 8T Manual Rev 050817 1 Table of Contents SETUP nana rn rider irse 4 CT ONC TIS E IO 4 ASSCHID me 4 OD VEEMMOW SENSOR TRETEN I E T T em 5 CONTROL DESCRIPTION iiiiick tetinsiieeesvbs eu ran 6 IQqnuiairirm 6 A mH 6 Command Pul 102 O10 7 o es 7 giga 7 UU C 7 qi 24 DEI E A ee EXA iia S PV CG OU A ec cs EA AP A nn A TESSE ae S INSTRUCTIONS TOR U Ensenada 9 Flow Adjustment eR S O uhiRDseRR BREI 9 NEAINTENANCE OU a 9 CT AMM m 9 ACPPEND Aira EA EEEE AEE EES 10 ATE T11 E A NEENA O NNE E E 10 SPECIEI ON unio E 11 O an0 I E TT E AEN E E E 12 Warner Instruments A Harvard Apparatus Company VC 8M Manual Rev 050817 1 3 The VC 8T Valve Control System lies at the heart
4. Y IN EXTERNAL ANALOG IN and EVENT OUT Valves A 15 pin D type female connector is used to connect the cable from the VALVE BRACKET to the CONTROLLER Warner Instruments A Harvard Apparatus Company VC 8M Manual Rev 050817 1 8 Overflow In This BNC is used to connect the OVERFLOW SENSOR to the controller Place the sensor into position and plug it into this BNC Standby In This BNC is provided to allow the placing of the VALVE CONTROLLER into standby mode Standby mode disables EXT ANALOG IN inputs and closes all valves Activation of this input is selected by placing the FRONT PANEL COMMAND input toggle into external analog mode and a TTL level high 3 5 V places the instrument in standby Ext Analog In This BNC is used to operate valves using analog voltage settings The voltage applied to this input selects the valve to open All other vales are closed Switching voltages closes the open valve and open the other Valve selections are overridden by the OVERFLOW SENSOR and the STANDBY BNC The table below indicates the selection scheme Applied voltage V Valve selection BENE GN Event Out The Event Marker output produces a 500 ms logic level output 5 V each time a valve is turned on At all other times the Event Marker output is low 0 V Power input module A polarized 3 conductor IEC320 CEE 22 connector is used for line mains power input to the instrument A removable cordset terminated with
5. e national laws of each EU Member State Once the final national regulations have been put into place recycling will be offered for those Harvard Apparatus products which are within the scope of the WEEE Directive Products falling under the scope of the WEEE Directive available for sale after August 13 2005 will be identified with a wheelie bin symbol Two Categories of products covered by the WEEE Directive are currently exempt from the RoHS Directive Category 8 medical devices with the exception of implanted or infected products and Category 9 monitoring E and control instruments Most of Harvard Apparatus products fall into either Category 8 or 9 and are currently exempt from the RoHS Directive Do Not Dispose Product with Harvard Apparatus will continue to monitor the application of the RoHS Municipal Waste Directive to ils products and will comply with any changes as they apply Special Collection Disposal Required Warner Instruments A Harvard Apparatus Company
6. from the stopcocks to the input ports on the PTFE valves Attach one 1 blunt end 18 gauge syringe needle to both ends of each length of PTFE tubing to provide Luer connection points Insert a short section of PE 160 onto the needle tip to facilitate a tight seal between the PTFE tubing and the syringe needle Using the Luer ended PTFE tubes formed in step 5 make a connection between the stopcock on each syringe to the associated input port on each PTFE valve Proper tubing lengths Luer connector attachments and tubing placements are shown in the montage above Cut eight 8 pieces of PTFE tubing to run from the output ports of the PTFE valve to the supplied MPP manifold The tubing length needed is left to the discretion of the user but should be sufficient to allow a short connection between the manifold and the sample chamber Attach the remaining blunt end syringe needles to each piece of PTFE tubing Prepare the MPP manifold to accept the PTFE tubing by first sliding a short length of PE 160 tubing over the input ports on the MPP manifold Now make a connection between the output port on each PTFE valve and one input port on the MPP manifold Run a short length of PE 160 tubing from the output port on the MPP manifold to the input port of sample chamber 12 Finally connect the VALVE MANIFOLD to the VC 8 CONTROLLER using the attached cable Overflow sensor The VC 8T Valve Control S
7. more than one valve at the same time from a digital source such as your computer Placing the ON OFF TOGGLE SWITCH into the on position places the associated valve into its open state A lit LED indicates the open state of the valve This setting is overridden by the overflow sensor Warner Instruments A Harvard Apparatus Company VC 8M Manual Rev 050817 1 7 Placing the ON OFF TOGGLE SWITCH into the off position places the valve into its closed state An unlit LED indicates the closed state of the valve Placing the ON OFF TOGGLE SWITCH into the ext position activates the associated BNC inputs A logic level low O V applied to the BNC places the associated valve into the closed state Correspondingly a logic level high 3 5 V places the associated valve into the open state A lit and an unlit LED indicates the open and closed states of the valve respectively These settings can be overridden by the overflow sensor Command input toggle The COMMAND INPUT TOGGLE is used to select between the front panel external digital controls and the rear panel external analog controls Overflow The OVERFLOW LED indicates an overflow condition sensed by the OVERFLOW SENSOR Activation of this circuit by an overflow condition immediately overrides all other settings and closes all valves Rear Panel The rear panel contains the power input module with fuse a 15 pin D connector for the VALVE SET and 4 BNC connections for OVERFLOW IN STANDB
8. of a multi valve perfusion system designed to automate and control the delivery of solutions to Warner Instruments imaging and recording chambers However its flexible design allows the VC 8T to be used in many applications not using Warner equipment Features include 8 channel individually controlled channels PTFE valves Spill sensor Manual and computer control No oS X 0 amp The complete system includes a valve controller a valve bracket with PTFE valves connecting cable an MPP 8 manifold a support stand syringe holder eight 60 cc syringes 25 feet of PTFE tubing 10 feet of PE 160 24 blunt end 18 gauge syringe needles and 8 stopcocks A new and exciting feature to the VC 8 Valve Control System is the Spill Sensor Probe This probe is used to detect the presence of an overflow condition and when activated will automatically shut off all valves protecting your microscope Optics The controller can independently regulate the function of up to eight valves Individual valves can be controlled via manual switch an external analog signal or an external digital TTL signal An event marker pulse generated each time a valve is switched on is provided at the rear of the instrument for recording into your acquisition system Valve transitions opened or closed occur at full power to insure rapid response times and are then held in place at less than half power to prevent heat transfer to solutions Warner Instruments A Ha
9. rom defects in materials and workmanship for a period of two years from the date of shipment If a failure occurs within this period we will repair or replace the faulty component s at our discretion This warranty does not cover failure or damage caused by physical abuse or electrical stress e g exceeding specified input limits Shipping charges to the factory are the customer s responsibility Return shipping of the repaired unit will be paid by Warner Instruments Inc Warner Instruments A Harvard Apparatus Company VC 8M Manual Rev 050817 1 Specifications Valve Bracket Valves Connection Cable Tubing Reservoirs Reservoir Holder Valve Controller front panel Power Input Select Switch Selection External Input Overflow Valve Controller rear panel Overflow In Standby In Ext Analog In Event Marker Manifold Power Operating Temperature Dimensions H x W x D Weight Shipping Weight Operating conditions Equipment is intended to be operated in a controlled laboratory environment Delrin Mounts on 3 8 or 1 2 ring stand PTFE 12 VDC 0 25 A to maintain pinch 2 4 meter 8 ft connecting cable terminated with quick disconnects on valve end and 15 pin male D type connector on controller end PTFE 1 8 OD x 1 16 ID 60 cc capacity syringes Delrin Holds eight syringes with thumb screws for each reservoir Two position on off with power on LED Two position selecting analog or digital inp
10. rvard Apparatus Company VC 8M Manual Rev 050817 1 4 SETUP Components Before beginning setup take inventory of the supplied components You should have a valve bracket with included PTFE valves a syringe holder support stand blunt end syringe needles 24 stopcocks 8 PTFE tubing 25 ft PE 160 tubing 10 ft an MPP 8 port manifold and a VC 8 Valve Controller syringe syringe holder stopcock Teflon tubing VC 8 Valve Controller valve bracket support stand sif The VC 8T is designed to operate as a stopped flow device wherein the valve for each channel is either open allowing solution to flow or is closed In general the shortest response time for delivery of the selected solution will be achieved by keeping the tubing length between the MANIFOLD and sample as short as possible Assembly 1 Begin assembly of the VC 8T by first attaching the VALVE BRACKET to the SUPPORT STAND Place the VALVE BRACKET near the base of the SUPPORT STAND as shown above 2 This is followed by attaching the SYRINGE HOLDER to the top of the SUPPORT STAND Place the SYRINGE HOLDER near the top of the SUPPORT STAND as shown above 3 Remove the plungers from the eight supplied 60 cc syringes and place syringes into the SYRINGE HOLDER Attach STOPCOCKS to each syringe Warner Instruments A Harvard Apparatus Company VC 8M Manual Rev 050817 1 10 11 Cut eight 8 pieces of PTFE tubing long enough to run
11. uts On manual Off or External 5 V TTL compatible BNC Connector LED indicating overflow condition BNC connection to OVERFLOW SENSOR Triggered when sensed resistance falls below 10 MQ BNC connection to activate standby mode TTL high closes all valves BNC connection for valve selection Active valve opened by analog voltage Logic level pulse 500 ms nominal BNC connector 8 1 nominal dead space 110 130 or 200 250 VAC single phase 50 60 Hz 20 watt 10 40 C 50 104 F 89 x 203 x 305 mm 3 5 x 8 0 x 12 in 3 7 kg 8 Ib 4 6 kg 10 Ib Temperature 0 40 C Altitude sea level to 2000 m Relative humidity 0 95 Warner Instruments A Harvard Apparatus Company VC 8M Manual Rev 050817 1 Certifications Declaration of Conformity CE MARKING EMC Application of Council Directive 89 336 EEC Standards To Which Conformity Is Declared Manufacturer s Name Manufacturer s Address Equipment Description Equipment Class Model Numbers EN55022 Class A EN61000 3 2 EN61000 3 3 EN50082 1 1992 EN61000 4 2 EN61000 4 3 ENV50204 EN610000 4 4 EN610000 4 8 EN610000 4 11 Warner Instruments LLC 1125 Dixwell Avenue Hamden CT 06514 Tel 203 776 0664 Valve Controller ITE Class A VC 8 Controller I the undersigned hereby declare that the equipment specified above conforms to the above Directive s and Standard s Place Hamden Connecticut USA Signature Full Name
12. ystem includes a unique overflow or spill sensor designed to assist you in providing a level of protection to your microscope The sensor itself is a small Warner Instruments A Harvard Apparatus Company VC 8M Manual Rev 050817 1 6 Ag AgCl probe which grounds through the chamber bath A resistance of 10 MQ or less between the probe and ground will trigger the overflow circuitry Place the probe adjacent your perfusion chamber such that the maximum solution height say just prior to an actual overflow is achieved when the solution contacts the tip of the probe Contact between the probe and your solution will activate the overflow circuitry and close all the valves The OVERFLOW SENSOR plugs into the OVERFLOW IN BNC on the rear of the controller CONTROL DESCRIPTION Front panel The front panel of the VC 8 Valve Controller contains TTL inputs for each valve an associated 3 position toggle switch for manually setting the state of each valve and an LED displaying the active status for each valve There is also a 2 position toggle switch for selecting the command input mode a SPILL SENSOR indicator and a power switch with power on LED TTL Inputs TTL inputs front panel BNC s and ON OFF TOGGLE SWITCHES are provided for all 8 channels of the VC 8 Controller Operation of the front panel TTL inputs is selected by placing the 2 position COMMAND INPUT TOGGLE into external digital mode Use of these inputs can allow the opening of
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