User manual - Texas Scientific Instruments, LLC
Contents
1. If an electrode without cables such as petridish and needle electrodes is used please go to step 6 Otherwise please go to step 8 A C115CB 6 Connect the male connector of a hook cable C117 to the Ss Baaler connector connector cables C115CB Refer to Fig 3 4 7 Hook up a hook of a hook cable C117 to the electrode Ze Refer to Fig 4 b C117 Male connector 8 Connect the electrodes such as tweezers electrode to the connector cable C115CB Refer to Fig 5 Fig 3 C115CB and C117 C117 Hook connecter C117 and chopstick type electrode D P i T 2 By S N y Pull up the top cover of C117 when hooking it WN A up to an electrode E o C117 and Petridish type electrode Fig 4 Connection of C117 hook connecter C115CB Female connecter ao BN d CUY650P5 e Fig 5 C115CB and tweezers type electrode CUY21SC USER MANUAL 5 Getting Started 5 1 Front Panel Model CUY21 Single Cell Parameter windows 12 Name Description 1 Set V Display the programmed voltage in 0 1V resolution 2 Measurement voltage Display the actual voltage in 0 1V resolution If the problem occurs during the measurement the measurement voltage will be displayed as a sign 3 Measurement current Display the actual current in 1mA resolution when current lt 1 00A or in 10mA resolution when current 21 00A If the problem o2. the short beep sounds twice Note If the long beep sounds once the entered value is out of range and will not be saved Enter the appropriate value again 10 Repeat step 6 9 if other parameters should be edited 12 CUY21SC USER MANUAL 6 Safety function For safety operation CUY21SC offers the safety limit function which protects one from electrical accident during the experiment As CUY21SC is designed for in vivo electroporation one must grab and manipulate electrodes by hand so there is high risk of electrical accident during the experiment To avoid such an unfortunate accident safety function is built in CUY21SC When current exceeds the specified limit 1 60A the device shuts off DC pulses immediately and automatically 13 CUY21SC USER MANUAL 7 Contact NEPA GENE CO LTD 3 1 6 Shioyaki Ichikawa Chiba 272 0114 Japan Tel 81 47 306 7222 Fax 81 47 306 7333 Email info nepagene jp Web http www nepagen jp Nepa Gene Co Ltd 2006 9 29 14
3. Sguare wave pulse electroporator CUY21SC User manual Cycle NEPA GENE CO LTD THIS PAGE LEFT INTENTIONALLY BLANK d WW Aan NO 1 Ree EE 2 JAJAN EE 3 31 Unpacking sasada aaa aa Ak ng Ng TA Ga LA Ka aa ee ee ee 3 3 2 Components and part NAMES asas eaaa eaaa eaaa eaaa eaaa eaaa anana anaa anaa anara nnee 4 4 PrecautionS titi abra A KAG KENE ya BANG dai 5 AA TE EE 6 E EE 8 Ov Front Eat EE PG Ai 8 e LTE 10 53 Operation a 11 5 3 1 Create new program cccococcccnnccccnoncnonnncnnnnncnnn nn nan cnc aaa aane naa anane anana nana ran 11 5 3 2 Impedance measurement aaa aaanaea aaa ntt trst aan anaa anana anana aana e nane n enere 11 5 3 3 Recall Electroporation ccccccccececceeeeceeeceececeeeeeceeeeeeeeeaeeeeeceeeseseaeeeeeeeieeeeeteas 12 SoA Edit the Program saves kaka aksa ia air E eee Meta 12 6 Safety TUM ON as aka Sede il KA dee lea AKA E Na na NA a aa aas 13 Teer El EE 14 CUY21SC USER MANUAL 1 Warranty Limited warranty for products Nepa Gene Co Ltd warrants that its products as delivered shall conform to its specification and be free of defects in materials and workmanship when used in accordance with their intended use This warranty is limited to twenty four 24 months from the product s original date of shipment from Nepa Gene Co Ltd The warranty for non durable products is limited to thirty 30 days from the product s original date of shipment from Nepa Gene Co Lt
4. ccurs during the measurement the measurement current will be displayed as a sign CUY21SC USER MANUAL 4 On Time Display the programmed pulse length of each square wave pulse in 0 01ms resolution When the entered value is more than 10 00ms only the first decimal place is displayed on On Time parameter window ie If pulse length is set to 50 25ms it is displayed as 50 2ms on the parameter window 5 Off Time Display the programmed pulse interval between square wave pulses in 0 1ms resolution When the entered value is more than 100 0ms the integer number is displayed on Off Time parameter window ie If pulse interval is set to 100 1ms it is displayed as 100ms on the parameter window 6 Cycle Display the programmed number of square wave pulses up to 99 cycles Control panel Name Description 7 Indicators Indicate the mode or parameter to be edited See 5 2 Indicator 8 Start Execute the impedance measurement or program 9 Main display Display the measured impedance value current value of selected parameter or program number 10 Stop Clear Delete the entered value and cancel the edit 11 Numerical key Enter the numerical value 12 Select Select the impedance measurement mode or parameter to be modified 13 Set Enable the edit of the parameters When Set button is pressed the selected parameter can be edited 14 Ente
5. clusive remedy for nonconforming goods shall be replacement of defective goods or at Nepa Genee option refund of the purchase price to customer The parties acknowledge that the price of Nepa Gene Co Ltd s products would be much greater if Nepa Gene Co Ltd undertook more extensive liability Any action by customer for any alleged breach of the warranty stated in this section must be brought within 90 calendar days after the end of the warranty period Notice Products returned to Nepa Gene Co Ltd for repair or replacement shall be received prepaid If found not to be defective under the terms of warranty a charge will be made for repair or replacement and freight costs will be at customer s expense Specifications are subject to change without notice For this reason and at its sole discretion Nepa Gene Co Ltd reserves the right to upgrade products during a repair process Prices for out of warranty repairs are subject to change without notice User name Invoice date Description Model Serial number 2 Specification CUY21SC USER MANUAL DC pulse Waveform Square Voltage 0 1 99 9V in 0 1V resolution Pulse length 0 05 99 9ms in 0 01ms resolution Pulse interval 0 1 999ms in 0 1ms No of pulses 1 99 Miscellaneous Impedance measurement Up to 39 9KQ Voltage measurement 0 1 99 9V Current measurement 0 001 1 60A When current lt 1 00A 1mA resolution When curr
6. d Further this warranty dose not apply to modifications made to the products by customer or damage to products resulting from modifications or repairs effected by customer or other personnel not authorized by Nepa Gene Co Ltd to perform such work This warranty shall not apply to damage resulting from loss or damage in transit II unreasonable use II customer s negligence or IV accident Nepa Gene Co Ltd reserve the right to examine the alleged defective goods to determine whether the warranty is applicable Limited warranty and disclaimer Nepa Gene Co Ltd warrants that all services supplied hereunder will be performed in a workmanlike manner except for the express warranties provided to customer under the above limited warranty for products Nepa Gene Co Ltd makes no other warranties or conditions expressed or implied as to the services or parts supplied hereunder and Nepa Gene Co Ltd expressly disclaims all warranties or conditions of merchant ability or fitness for a particular use Limitation of liability Nepa Gene Co Ltd liability under this agreement is limited to the expense of providing product repair neither Nepa Gene Co Ltd nor its employees or agents shall be liable for indirect special incidental or consequential damages including without limitation business interruption lost profits or revenues wages or injury to persons or property customer and Nepa Gene Co Ltd agree that the sole and ex
7. e Programmed parameters will be displayed on parameter windows 4 Press Start button 5 Electroporation program will be executed The short beep sounds twice and the word End is displayed on the main display after the program is completed Note Make sure that the indicators other than impedance measurement mode should be selected If the impedance measurement mode is selected the impedance measurement will be executed Do not touch the uninsulated connections and components of an electrode WARNING S and cable when power is present 6 The actual current and voltage will be displayed on parameter windows respectively a 3 4 Edit the program 1 Turn the power on Select memo by pressing Select button LED light beside memo will be turned on Press Set button once LED light beside memo will blink and main display will be blank Enter the program number to be edited by using the numerical keypad Press Enter button once The short beep will sound twice Select the parameter to be edited by Select button E OS Press Set button once LED light for the selected parameter will blink and main display will be blank Note If you would like to cancel the programming press Stop Clear button once 8 Enter the desired value by using the numerical keypad Note If you would like to cancel the entered value press Stop Clear button once 9 Press Enter button once When the value is approved and saved
8. e used in this user manual in order to make use of the instrument and prevent the instrument from being damaged Follow the instructions marked with the symbol N CAUTION Failure to follow the guidelines marked with this symbol could result in minor personal injury or product and or peripheral damage A WARNING Failure to follow the guidelines marked with this symbol could result in severe personal injury or death A WARNING Do not bend the power cable forcibly or place heavy object on the cable because it might damage the cable and cause fire or electric shock If the power cable is damaged discontinue use and replace it immediately Do not open the cover of the device when the power is present Only qualified personnel should perform service procedure Keep the inflammable materials away from the device Do not touch the uninsulated parts of cables and electrodes when power is on AN CAUTION Do not drop or hit the device Do not drop any metallic objects or liquids on to the device Be sure to hold the connector part of the power cable when disconnecting the cable Pulling on the cable portion may cause it to fray and break Do not operate the device in any of the following cases There is physical damage to the device Smoke strange noise or smells erupt from the device Contact the nearby supplier immediately Disconnect power cable from the device if the device is not
9. ent 21 00A 10mA resolution Safety limit 1 60A See 6 Safety function Memory Up to 99 programs Power 115 0r220V 2 5A 50 60Hz Fuse 5A Dimensions W360mm x L380mm x H180mm Weight 12 3Kg CUY21SC USER MANUAL 3 Introduction Thank you very much for purchasing CUY21SC square wave pulse electroporator Before using this product please read this Instruction Manual thoroughly so that you can use this product at its full functionality After reading retain this manual in an easily accessible location so that you can refer to this manual whenever you have a problem or trouble during use 3 1 Unpacking Remove all packing materials and components from a carton CUY21SC should arrive complete with the following components 1 x Main unit 1 x Footswitch cat No C200 1 x Connector cables red and black cat No C115CB 1 x Hook cables red and black cat No C117 1 x Power cord 1 x Dust cover 1 x Operation manual If any item of the above list is not included contact the local supplier nearby CUY21SC USER MANUAL 3 2 Components and part names FRONT Parameter windows See 5 1 Front panel Control panel See 5 1 Front panel Voltage output Connector for footswitch remote switch REAR Fuse Terminal for oscilloscope Input output 10 1 Power cable connector Ventilation fun RS232C unused CUY21SC USER MANUAL 4 Precautions The following symbols ar
10. r Save the entered value and execute the recall of the program 5 2 Indicator CUY21SC USER MANUAL o gel A Impedance measurement mode B Parameters J meng C Memory Indicator Description A Impedance measurement mode Q Impedance of sample and buffer can be measured prior to electroporation B Parameters Applied voltage can be entered and edited in the range from 0 1V to 99 9V in 0 1V resolution When this indicator is selected the current value of this parameter is displayed on the main display Pon Pulse length can be entered and edited in the range from 0 05ms to 99 9ms in 0 01ms resolution When this indicator is selected the current value of this parameter is displayed on the main display Poff Pulse interval can be entered and edited in the range from 0 1ms to 999ms in 0 1ms resolution When this indicator is selected the current value of this parameter is displayed on the main display Cycle No of pulses can be entered and edited from 1 up to 99 cycles C Memory Memo The specific number of the program can be selected and recalled Program number is displayed on the main display i e Ch 5 10 CUY21SC USER MANUAL 5 3 Operation 5 3 1 Create new program 1 2 3 4 EA 10 11 12 13 14 15 Turn the power on Select memo by pressing Select button LED light beside memo will be turned on Press Set b
11. used Use proper fuse specified for this product CUY21SC USER MANUAL 4 1 Installation 1 Place CUY21SC on a level dry and clean surface near an appropriate electrical outlet and N CAUTION Do not install CUY21SC in areas that are 1 damp humid or very dusty 2 exposed to direct sunlight 3 poorly ventilated 4 subject to extreme temperature or humidity changes e g near an air conditioner or heater Be sure to allow the required space behind the instrument for proper ventilation 2 Check that the voltage indicated on the rear panel of the unit meets the local power requirements AN CAUTION Use the proper power cable specified for the device 3 Connect the power cord to the receptacle on the rear panel of the unit and plug the unit into the appropriate electrical outlet AN CAUTION Make certain the outlet is properly grounded sent before operation 4 Connect the foot switch C200 to the connector on the front of the unit Refer to Fig 1 4 WARNING Ensure that the main power of the device is turned off Fig 1 BNC connecter of Footswitch 5 Attach the connector cables C115CB to the output connecter on the front of the device Refer to Fig 2 Note We recommend that the cable should be connected to the output connector whose color is matched with that of the cable d Fig 2 C115CB male connecter CUY21SC USER MANUAL
12. utton once LED light beside memo will blink and main display will be blank Enter the program number by using the numerical keypad where the new program will be saved Press Enter button once The short beep will sound twice Select V by Select button Press Set button once LED light beside V will blink and main display will be blank Note If you would like to cancel the programming press Stop Clear button once Enter the applied voltage by using the numerical keypad Note If you would like to cancel the entered value press Stop Clear button once Press Enter button once When the value is saved the short beep sounds twice Note If the long beep sounds once the entered value is out of range and will not be saved Select Pon by Select button Repeat step 6 8 Select Poff by Select button Repeat step 6 8 Select cycle by Select button Repeat step 6 8 5 3 2 Impedance measurement 1 2 3 Turn the power on Press Select button until the LED light beside Q is turned on Press Start button once Impedance measurement will begin and value will be displayed on the main display Note If the impedance is beyond the range 39 9K 2 make sure that the cables are properly connected 11 CUY21SC USER MANUAL 5 3 3 Recall Electroporation 1 Select memo by Select button 2 Enter the program number to be recalled 3 Press Enter button once The short beep will sound twic
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