HS2000 Xcalibur Setup Rev D
Contents
1. Stand by temperatures Oven temperature C 4 Analysis time lt HS2000 Sample Speed Sample draw ml fo Filling speed ml min fi HS2000 3 Enrichment fi Injection speed ml min fi Aut l d ale 5 Enrichment delay min fp 3 Injection delay Oven incubation mode Pre injection delay sec o Constant incubation x Post injection delay sec o JES EE Oven incubation settings 2000 i m Syringe C 40 Temperature C Temperature C ao Incubation time min fo F Enable pre filing I Enable flush Progressive incub time min fp EE 0 Shaker time on min fo Filling counts o Shaker time off min o Filling delay sec o Multiple extraction counts 1 v 0 0 Total GC cycle time min fo Syringe temperature C 4 Figure 1 2 HS 2000 Page Sample Group Boxes Sample Group Box This group of controls is used to set parameters for the sample draw and for use with the Multiple Headspace Extraction MHE option The amount of sample volume to be injected The number of MHE enrichments to make Read the HS 2000 operating manual for more detailed information The delay to flush the vial with carrier gas in the MHE technique See the HS 2000 operating manual for more details Speed Group Box This group of controls contains the parameters to control the speed the sample is drawn into the syringe and2. Druivenstraat 33 NL 4816 KB Breda 31 076 587 8722 P R China Thermo Finnigan China Suite 912 916 Ping An Mansion No 28 Jin Rong Street Xicheng District Beijing 100032 86 010 6621 0839 Spain Thermo Finnigan SA Acer 30 32 Edificio Sertram Planta 2 Modulo 3 ES 08038 Barcelona 34 093 223 0918 Spain Thermo Finnigan SA Avenida de Valdelaparra 27 Edificio Alcor Planta 2a ES 28108 Alcobendas Madrid 34 091 657 4930 Sweden Thermo Finnigan AB Pyramidbacken 3 S 141 75 Kungens Kurva Huddinge 46 08 680 0101 United Kingdom Thermo Finnigan Ltd Paradise Hemel Hempstead Herts HP2 4TG 44 01 442 233 555 Notes The country code is enclosed in square brackets The city code or area code is enclosed in parenthesis For countries other than the U S A when you are dialing from within the specified country dial the 0 of the city code For countries other than Italy when you are dialing from outside the country do not dial the O of the city code CopyrightO 2002 Thermo Finnigan a member of the Thermo Electron family of companies All rights reserved Printed in Italy K Contents Contents Chapter 1 AAA o EE 5 AEE SILO orita ET 6 Configuration Screen 6 162 ES ZOO Memo Te GION os TE E E 8 Sample Group Boesak ke ee Oe ses 8 Incubation Grou BOXES Re EE es ie oe ae ee EE Di oe eo 9 Syringe Group BOXES sesse RR ee
3. SEER SR Ge ee Ee RES EE ee Ge ies 10 Stand by Conditions Group BOXES ie ees ee RR EE EE ee ee ee ee ee ee 11 HS 2000 Command Menti sesse aiii bla 12 Lo Seine Up HS 2000 Pataf letS os ER Ee es EE ear 13 Thermo Finnigan HS 2000 Head Space Autosampler iii Contents SSSSSSSSlCl S S SO y Sz K IV HS 2000 Head Space Autosampler Thermo Finnigan Thermo Finnigan Chapter 1 HS2000 Set Up View This chapter contains informations to configure and to set the HS 2000 Head Space Autosampler Parameters This chapter contains these topics Configuration 6 HS 2000 Method Editor 8 Setting Up HS 2000 Parameters 13 HS 2000 Head Space Autosampler User Manual 1 5 HS2000 Set Up View Configuration Serial Port Normal Anticipated Ctrap Norm Ctrap A St K 1 1 Configuration Configuration Screen Items listed under this heading contain definitions to each control you see listed in this Instrument Configuration window H52000 Configuration Communicator DE Serial port A Cancel GC start mode fe Moral f Anticipated Ctrap A St Help FEEL Ctrap norm Springe Syringe volume ml FO wt Firmware Version IY Firmware version 3 3 or above Figure 1 1 HS 2000 Configuration Screen Communication Group Box This parameter specifies the communication port to be used for the Sampler it can be through the GC or alternatively can be any COM Port from to 8 GC Start m
4. 2000 Configuration the Firmware version 3 3 or above box has been checked a check box is displayed as shown below Click on this check to enable disable the control of the temperature HS 2000 Head Space Autosampler User Manual 1 11 HS2000 Set Up View HS 2000 Method Editor Stand by temperatures M Oven temperature Ce 50 M Syringe temperature C 50 Syringe temperature Select a temperature for the syringe to stay while the autosampler is not C running Note Ifin HS 2000 Configuration the Firmware version 3 3 or above box has been checked a check box is displayed as shown below Click on this check to enable disable the control of the temperature Stand by temperatures M Oven temperature Ce 50 M Syringe temperature C 50 HS 2000 Command Menu Take your choice in using your mouse to access the autosampler menu commands Send Method to This function transfers the analytical method from the memory of the GC to Sampler the autosampler and enables the method Get Method from This function allows to transfer method data from autosampler to GC Sampler Import command Loads an autosampler method as from an earlier version of GCQ software 2 1 or earlier 1 12 HS 2000 Head Space Autosampler User Manual Thermo Finnigan Thermo Finnigan HS2000 Set Up View Setting Up HS 2000 1 3 Setting Up HS 2000 Parameters For all the incubation modes l From the Syringe flush group box s
5. S configuration This box enable disable the pre filling mode This function allows the vial to be pressurized before sampling the headspace vapors This function must be set only in cases of sample enrichment that 1s when the number of samplings from the same vial is higher than 1 The volume used to pressurize the vial is the syringe volume Specify the sample volume to intially pull up into the syringe Subsequently the autosampler pushes the plunger down to the actual sample volume injected Specify the number of sample rinses of the syringe Enter a number from 1 to 15 Specify the delay in seconds the plunger is held at the Sample Volume while pre filing the syringe Injection delay Group Box The delays are the amount of time the syringe needle resides in the injector before or after the sample 1s injected Enter the time in seconds to hold the syringe needle in the inlet before injecting the sample A delay of more than a few seconds will preheat the needle Enter the time in seconds to hold the syringe in the inlet after injecting the sample A short delay may be used if disruption of the injection appears to occur when the syringe 1s pulled out too early Stand by Conditions Group Boxes Standby temperatures group box The parameters in the group box permit to enter the stand by conditions of HS2000 Select a temperature for the incubation oven to stay while the autosampler is not running Note Note If in HS
6. Thermo Finnigan TRACE GC HS 2000 Head Space Autosampler User s Manual Published by Technical Publications Thermo Finnigan Italia S p A Strada Rivoltana 20090 Rodano Milan Italy Printing History Revision D printed March 2002 Xcalibur and TRACE GC are trademarks and or product names of Finnigan Corporation Microsoft is a registered trademark of Microsoft Corporation Technical information contained in this publication is for reference purposes only and is subject to change without notice Every effort has been made to supply complete and accurate information however Thermo Finnigan Corporation assumes no responsibility and will not be liable for any errors omissions damage or loss that might result from any use of this manual or the information contained therein even if this information is properly followed and problems still arise This publication is not part of the Agreement of Sale between Thermo Finnigan Corporation and the purchaser of a Thermo Finnigan system In the event of any conflict between the provisions of this document and those contained in Thermo Finnigan Corporation s Terms and Conditions the provisions of the Terms and Conditions shall govern Reference to System Configuration and Specifications supersede all previous information and are subject to change without notice The GC MS and LC MS products of the Thermo Finnigan Division are produced under VISION 2000 accredited quality management sy
7. action steps of head space from the same sample vial repeatedly performing an operation sequence Syringe Group Boxes Syringe Group Box This group box controls syringe parameters Since a heated syringe 1s used to transfer headspace gases from a vial into the injection port of the GC the temperature of that syringe can be controlled The range 1s 40 to 150 C One degree 1s the smallest increment Note If in HS 2000 Configuration the Firmware version 3 3 or above box has been checked a check box 1s displayed as shown below Click on this check to enable disable the control of the temperature Syringe M Syringe temperature EJ 50 Activating this control allows the syringe to enter the vial with the plunger in the top position therefore pressurinzing the vial before withdrawing the sample The syringe enters the vial which is filled with the sample volume of purge gas Use this function only if using sample enrichment When multiple samplings from the same vials are used the vial remains at constant pressure Check this box to download the method to the autosampler The method HS 2000 Head Space Autosampler User Manual Thermo Finnigan K Enable flush Filling volume ml Filling counts Filling delay sec Pre injection delay sec Post injection delay sec Oven temperature C Thermo Finnigan HS2000 Set Up View HS 2000 Method Editor downloads to method IS IP or IM depending on the H
8. is check to enable disable the control of the temperature W Temperature C AL Oven incubation settings This box specifies the total incubation time for all sample to be analyzed with the method being used The time here specified is in minutes 0 1499 min This box specifies the time added progressively to the incubation time for all sample to be analyzed with the method being used The time here specified is in minutes 0 599 min The number of minutes to shake the sample The amount of time for the shaker to be turned off HS 2000 Head Space Autosampler User Manual 1 9 HS2000 Set Up View HS 2000 Method Editor Multiple extraction counts Constant incubation Progressive incubation Multiple head space 1 10 extraction Temperature Enable pre filling K This box is accessible if Multiple Head Space extraction mode has been set Enter here the number of injections and vial headspace extractions that will be carried out from each vial Oven incubation mode This box permits to select the three incubation modes supported by the HS 2000 It allows the sample to be sequentially conditioned at a programmed temperature with a constant conditioning time It allows the sample to be sequentially conditioned at a programmed temperature with a conditioning time that increase for each sample according to a programmed additional time Dt Refer to Progressive delta It allows automatic multiple extr
9. ode group box This parameter specifies the synchronization mode between the Sampler and the GC This start mode permits to start the GC program when the needle enters the Injector This start mode permits to start the GC program when the needle enters the Injector The Ctrap norm and Ctrap A St options are purposely provided for the cold trap synchronism The meaning of Norm and A St remains the same as above HS 2000 Head Space Autosampler User Manual Thermo Finnigan K Syringe volume Firmware Version Thermo Finnigan HS2000 Set Up View Configuration Syringe group box This parameter specifies the volume of the syringe in use This parameter is then used in the HS2000 method editor for maximum volumes check Check the Firmware version 3 3 or above box if your HS 2000 is equipped with the 3 3 or above firmware version Click OK after you are satisfied with your selection Click Cancel if you would like to exit without saving your changes Click Help for this page to display HS 2000 Head Space Autosampler User Manual 1 7 HS2000 Set Up View HS 2000 Method Editor Sample draw ml Enrichment Enrichment delay min K 1 2 HS 2000 Method Editor The Method Editor screen contains all of the parameters used to set up injection events Click the links below to find out more about the parameters E Senza titolo Instrument Setup loj x File H52000 Help osm S x
10. pecify which flush parameter to use One Minute or Permanent In the Stand by temperature group box specify values for the Oven temperature and the Syringe temperature In the Injection Delay group box specify values for the Pre injection delay and the Post injection delay parameters In the Sample Incubation Oven group box specify values in these parameters Temperature Incubation time and Progressive incubation time Shaker time on and the Shaker time off In the Start mode group box select either Normal or Anticipated In the Speed group box specify values in these parameters Filling speed and Injection speed In the Sample group box specify values in these parameters Sample Enrichment Enrichment delay In the Syringe group box specify values in these parameters Temperature Filling Volume Filling counts and Filling delay Check the Enable pre filling parameter if using the Multiple Headspace Extraction M mode HS 2000 Head Space Autosampler User Manual 1 13
11. stems U S A Thermo Finnigan Corporation 355 River Oaks Parkway San Jose CA 95134 1991 1 408 965 6000 Thermo Finnigan Technical Support 3661 Interstate Park Road North Riviera Beach FL 33404 800 685 9535 561 844 5241 Fax 561 881 8431 Australia Thermo Finnigan P O Box 239 Rydalmere Unit 20 Metro Centre 38 46 South Street Rydalmere N S W 2116 61 02 9898 9000 Austria Thermo Finnigan GmbH Wehlistrasse 27b A 1200 Wein 43 01 333 50 34 0 info quest thermo at Belgium Thermo Finnigan BVBA Groenenbrogerlaan 84 B 2610 Wilrijk Antwerpen 32 03 825 0670 Canada Thermo Finnigan Canada 5716 Coopers Avenue Unit 1 Mississauga Ontario L4Z2E8 1 905 712 2258 France Thermo Finnigan France SA Parc Hightec Sud 12 Avenue des Tropiques Z A de Courtaboeuf BP141 F 91944 Les Ulis C dex 33 01 69 18 88 10 Germany Thermo Finnigan Analytische Systeme GmbH Boschring 12 D 63329 Egelsbach 49 06103 408 0 Italy Thermo Finnigan Italia S p A Strada Rivoltana 20090 Rodano Milano 39 02 95059 1 Japan Thermo Finnigan K K Nishi Shinjuku Toyokuni Bldg 3F 2 5 8 Hatsudai Shibuya ku Tokyo 151 0061 81 03 3372 3001 Japan Thermo Finnigan K K Esaka Grand Building 2 3 1 Esaka cho Suita City Osaka 564 0063 81 06 6387 6681 Netherlands Thermo Finnigan BV
12. the speed it is injected into the inlet HS 2000 Head Space Autosampler User Manual Thermo Finnigan K Filling speed ml min Injection speed ml min Total GC Cycle min Temperature C Incubation time min Progressive delta Shaker time on min Shaker time off min Thermo Finnigan HS2000 Set Up View HS 2000 Method Editor The speed the sample is withdrawn from the headspace into the syringe Speed of the injection in milliliters per minute of the sample into the inlet The speed should be at least as fast as the injector flow For split injections a fast speed reduces tailing Analysis Time group box The analysis time group box contains Set this time for the full cycle time of the GC analysis At the end of the run observe the amount of time it takes the GC to cool down and then go ready Add this time to get the GC cycle time After the first cycle this time 1s precisely calculated by the software for subsequent cycles Incubation Group Boxes Oven incubation settings group box This group of controls sets the parameters for incubating the sample The temperature to incubate the sample For any Headspace separation the appropriate incubation temperature 1s the most important parameter Here the temperature is selected from 40 to 150 C in increments of 1 C Note Ifin HS 2000 Configuration the Firmware version 3 3 or above box has been checked a check box is displayed as shown below Click on th
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