1100 SERIES
Contents
1. Bh Ed Vow Bo Facts VOR UP aa E n Po ee Sane EU TENER nmm ET p Am im BMyComputer 95 4 m po nm oe ae Esha Er cia po m iem p traon oie ime Tma Mikoani La Pintore Carei Core eu E rc iai ei Free Space Cape GUMB E My Cempuler E Figure 2 Locate CD Drive Screen UNICO Application Software EDT ink C LooalDisk My Computer 2000 EIU Local Di HIS ata Figure 3 Open the CD APH Fie rima rio UST hie i or p 3 Size 138KB Figure 4 Click setup exe UNICO Application Software UNICO Application Software For VIS1100RS Setu peres gera poo aea Hiis eene aids vay Figure 5 Setup Screen UNICO Application Software For VI51100R5 Setu L pus caine Figure 6 File Setup Directory Selection UNICO Application Software 3 UNICO Application Software For VIS1200 Choose Pro PowerQuest BostMagic 7 0 PowerQuest ParitionMagic 7 0 Figure 7 File Setup Directory Selection Figure 8 Install Icon UNICO Application Software For VIS 1100 Figure 9 I2. Functions of Drop down Menu Text Field and Icon Button Drop down Menu Two Drop down Menus File Figure 14 and Application Figure 15 are illustrated below 10 UNICO Application Software mei Figure 12 Main Screen Display ce Figure 13 Main Screen Display After User Login Figure 14 File Drop down Menu UNICO Application Software Application A Tine Conc kinetics Standard Curve Figure 15 Application Drop down Menu File Drop down Menu has six functions New User New Load Save as Print and Exit All of them have hot keys except the first one New User change user name New reset Text Parameter and begin new test Load open any saved file tst Save as save the setup or data displayed Print print all information shown in the Data Screen and any data collected Exit quit the VIS1100RS Application Drop down Menu can select three Analytical Methods of the 110085 Spectrophotometer A Trans Cone Kinetics and Standard Curve measure Absorbance Transmittance Concentration Standard or Concentration Factor Kinetics measure a sample s Absorbance change over a selected period of time store the test results in data table and display the results graphically Standard Curve create a calibration curve choice of 4 curve fits with up to 8 standard solutions to determine Concentrations of unknown samples Text Field Three Text Fi
3. button at the right of button to set the wavelength Click on 081100247 Icon Button button the blue cuvette icon to blank the reference DO NOT PUSH ANY BUTTONS ON THE 1100RS THIS WILL CAUSE FOR YOU TO RE START THE SOFTWARE Remove the reference and place your sample cuvette into the Sample Compartment close the lid and click on TEST Button The test results will be displayed in a spreadsheet format in the Data Table You may type in sample name or change the sample name under the Name label inside the Data Table 16 UNICO Application Software EEE Delais aas animat 0898 mem na miar mancam Figure 25 Sample Test Results of Absorbance Transmittance Mode Concentration Standard Mode The purpose of this test is to determine the Concentration of the unknown samples by comparing the samples Absorbance Transmittance to that of the standard solution Repeat Step 1 to 3 in Absorbance Transmittance Mode section Step 4 Type in the known concentration of the standard solution in the Text Field at the right of Cone Std label Step 5A Place the standard in the Sample Compartment and close the lid Step 6A Click the OK Button in Test Parameter section This will measure the Absorbance of the standard and set its conversion Factor for measuring the unknown samples Figure 26 Step 7A Place your sample s in the Sample Compartment and click the TEST Butt
4. indicates Right click the CD Drive Label and click Open as shown in Figure 3 Click setup exe as Figure 4 shown or click SETUP shown in Figure 1 The Setup Screen Figure 5 will then display a Welcome message reminding you to close any open programs If all programs are closed then click OK and go to Step 6 If other programs are open and operating click Exit Setup close the open programs and return to Step 2 Choose the Directory and Program Group where you want the software to be installed as shown in Figure 6 and Figure 7 The File Setup Directory 1 Figure 6 will then appear If the File Path selection is OK then go to Step 7 NOTE if you wish to change the location of the installation then click Change Directory and specify the desired directory If you are unsure and need to examine your computer files click on Exit Setup and go to Windows Explorer to make sure no duplication or improper storage of the files will occur Setp 7 Step 8 Click the Install Icon Figure 8 to start installation VIS1100RS click Continue to finish the installation Wait for the message stating the software was installed successfully as shown in Figure 9 Click OK re start your PC Congratulations You have now installed your UNICO Application Software VIST100RS in your PC for your 110085 Spectrophotometer UNICO Application Software d Products amp Instrum Road Figure 1 Automatic Setup Screen
5. the UNICO Application Software VIS1100RS test data to a Microsoft Excel program Step 1 In Microsoft Excel click on File and click Open 25 Step 2 Step 3 UNICO Application Software Select any saved file you wish to import After the Excel Text Import Wizard appears select Delimited select the row from which you want the import to start and click on the Next button as shown in Figure 39 Uncheck the Tab delimiter and select Comma delimiter as shown Figure 40 then click the Next button Click the Finish button and the test data will be imported into your Excel spreadsheet Here further calculations can be performed from the raw data collected Save the imported file under a DIFFERENT FILE Name if you still want to keep and open the original UNICO data file in VIS1100RS Otherwise the original UNICO data file st file will be modified by the Excel format during importing and the modified file cannot be opened from VIS1100RS E Figure 39 Excel Text Import Wizard 26 UNICO Application Software Figure 40 Excel Text Import Wizard 27
6. If standard is available type the concentration values of the standard into the Text Field at the right of Cone Std label insert the standard into Sample Compartment and click the OK button Spectrophotometer will take measurement of the standard and display factor value The unknown sample concentrations can then be measured If factor value is available enter the factor value into the Text Field at the right of Factor label for the unknown sample concentrations calculation Figure 21 UNICO Application Software TEST Button record the readout of a given sample Figure 23 Data Table record ID Name Abs Trans Conc and Factor related with the test Figure 23 Test Date Test Date will be displayed automatically To alter it simply click it at the left corner of the screen and enter the desired date Figure 24 pre Figure 21 Test Wavelength and Test Parameter Figure 22 Wavelength and Reference Setting UNICO Application Software ii EE Figure 23 TEST Button and Data Table Please Input Test Date Figure 24 Test Date Absorbance Transmittance Mode The following are the basic operations Figure 25 Step 1 Step 2 Step 3 Step 4 Step5 Insert reference cuvette into the Sample Compartment and close the lid Select the desired wavelength by moving the WAVELENGTH dial on the 11008 and click button on the PC screen to match the desired wavelength click the OK
7. UNICO 1100 SERIES SPECTROPHOTOMETER SOFTWARE USER S MANUAL Version SS 1 11 United Products amp Instruments Inc 182 Ridge Road Suite Dayton NJ 08810 USA TEL 792 278 1185 FAX 7324 274 1151 http Awww unico1 UNICO Application Software Table of Contents Introduction Minimum Computer Requirements Software Installation Loading to Computer Connecting Computer to Spectrophotometer Main Screen Display Functions of Drop down Menu Text Field and Icon Button Basic Operation Absorbance Transmittance Concentration Main Screen of A Trans Conc Absorbance Transmittance Mode Concentration Standard Mode Concentration Factor Mode Standard Curve Main Screen of Standard Curve Kinetics Main Screen of Kinetics Data import to Microsoft Excel wo 10 10 13 13 14 16 17 17 19 19 23 23 25 UNICO Application Software Introduction The UNICO Application Software VIS1100RS has been designed to operate with UNICO Spectrophotometer Model 110085 The software runs on a personal computer with Windows 95 98 Me NT 2000 XP operating system installed The UNICO Application Software package includes One CD containing the software VIS1100RS Software User s Manual 6 modem connection cable with 9 pin and 25 pin female connector s on both ends The UNICO Application Software performs the following methods for analysis Absorbance Tra
8. e 30 Standard Sample Setting E re seem we co nalala sleis Ten a E connor Sea aa ra Figure 31 Test Results of Standard Curve UNICO Application Software fe pom Delale alale SE Figure 32 Standard Curve Graph Display Your standards have now completed setup To use the graph and measure the samples with unknown concentrations please be guided by the steps below Step 1 Select the desired Standard Curve by clicking on one of the four buttons Figure 33 Shown in Figure 32 is Linear Squares Least Squares Method Items in the equation next to the Linear Squares title on the graph are Abs the slope and y intercept as well as Correlation Coefficient Abs slope y intercept Corr Coef Correlation Coefficient Step 2 optional Click Print button print the graph and labels of the slope y intercept and Correlation Coefficient as seen on the screen Step 3 When ready to measure samples of unknown concentration press arrow return button to return to the main Standard Curve screen Figure 31 Step 4 To measure the concentration of unknowns place the samples in the Sample Compartment and click on Test button Figure 31 located at the portion of the screen Step 5 To save the data with the Standard Curv
9. e fit selected click on th Save Icon name the file and click Save een eee Figure 33 Buttons for Standard Curve Test 22 UNICO Application Software Kinetics The Kinetics application has the following functions Setup kinetics Test Parameters Obtain kinetics data for a sample at a single wavelength Load and save data files for further studies Main Screen of Kinetics At the Main Screen Figure 13 click Application Drop down Menu click Kinetics Figure 15 to enter the Main Screen of Kinetics Test Figure 34 ai Per ea ra Figure 34 Kinetics Test Main Screen The following are the basic operations Step 1 Insert reference cuvette into the Sample Compartment and close the lid Step 2 Select the desired wavelength by moving the WAVELENGTH dial on the 1100RS and click button on the PC screen to match the desired wavelength click the OK button at the right of button to set the wavelength Step 3 Click on 0 100 Icon Button 20 the blue cuvette icon to blank the reference Step 4 Set the Test Parameters Figure 35 Step 4 Step 8 Set the Total Run Time by typing into the Text Field on the right of the Total Run Time label This is the total time for the reaction to occur Step 5 Set Sampling Interval time similar as Step 4 This is the time interval for which measurements will be recorded i e
10. elds are just below File Drop down Menu and under the label Current WL nm Abs and Trans Figure 16 Ate Iz 000 Figure 16 Text Field Current WL nm Text Field display the last selected wavelength of the spectrophotometer Abs Text Field display the current data in Absorbance Trans Text Field display the current data in Transmittance 12 UNICO Application Software Icon Button Seven Icon Buttons are below the Text Field Figure 17 olem 01059 Figure 17 Icon Button The four Icon Buttons counting from the left end of the Figure 17 have the same functions as New Load Save as Print of the File Drop down Menu 0 100 Icon Button set 0 Absorbance and 100 T Insert reference into Sample Compartment and click lll button blue colored move cursor on to the blue cuvette icon the fifth icon from left end of Figure 17 and right touch the mouse it will show 0A 100 T sign Figure 18 87100 Figure 18 0A 100 T Icon Button 0 clicking Ed button black colored move cursor on to the black cuvette icon the sixth icon from left end of Figure 17 and right touch the mouse it will show 0 T sign Figure 19 Button set 0 T Remove Cuvette from Sample Compartment before Figure 19 0 T Icon Button Reset Button unlock the screen If the screen frozen due to improper operations BLANK may show in the Text Fields below Abs and T
11. et TER New lies Document tla CH Prassi PrimeCD Phir 2 0 rash Deen omes Domeni Y Va Epa 8 Menon inii B wren Ed PaiienMaie 70 LE 5laitup pesca fe 2 B3 TRADOS ZE TRADOS E Frocan oe semen E UNIC OApmieanon Sotware 102 ShutDown CA reren Praes eo Figure 10 Open the Software UNICO Application Software WARNING f you do not have a test tube or something in the Sample Compartment the light shutter will be closed the VIS1100RS will not work If you do not push the ENT the proper connection between the VIS1100RS and 1100RS will not be made and it will also cause problems when using the VIS1100RS CAUTION Do NOT use any button on the 1100RS panel while the VIS1100RS is running and connected with the 1100RS Otherwise the data collected may not be correct F UnicO sonare Please Select Communication Port pa 0 Figure 11 Start up Screen Main Screen Display After your 110085 is connected to your PC and VIS1100RS is running your will show the Main Screen Display like Figure 12 Type your User Name in the Text Field at the right of the User Name U label or leave it blank then click the OK button to login as Figure 13 shown
12. every 10 s or every 3 s etc Step 6 Step 7 Step 8 Step 9 UNICO Application Software Set an Initial Time Delay similar as Step 4 The purpose of this step is to delay the beginning of data collection i e Sample must be injected and reaction will not begin for 20 s Set a Cone Factor multiplication factor dilution factor similar as Step 4 This allows for a factor to be used when calculating the Concentration of the solution Set the High and Low Limits of the graph similar as Step 4 This is the selection of the minimum and maximum Absorbance range for the graph of the data Place your sample in the Sample Compartment and click the Start Button once all the Test Parameters have been set and you are ready to start your measurement Figure 36 Tenn o rA am ii Figure 35 Test Parameters Setting Notes Data Table once measurements have been made clicking this button wil allow you to view the data collected Figure 38 Only Cell 2 of the Data Table is currently used to store the data collected other are for future applications Stop Button stops the data collection at any given moment UNICO Application Software Figure 37 Sample Test Results of Kinetics Figure 38 Kinetics Data Table Data import to Microsoft Excel By referring to the following steps you can transfer any of
13. nsmittance Concentration measure the Absorbance Transmittance Concentration Standard or Concentration Factor at a single wavelength within the range of 335 1000 nm Standard Curve create a calibration curve choice of 4 curve fits with up to 8 standard solutions at a single wavelength to determine concentrations of unknown samples Kinetics Absorbance vs Time Kinetics measure a sample s absorbance change over a selected period of time store the results in a data table and display the results graphically Minimum Computer Requirements To properly install and operate the enclosed software it is required to have the following minimum computer configuration PC with lt 16 RAM Pentium or faster processor 10 MB of free space on memory VGA Color Monitor PS 2 mouse and keyboard NOTE The UNICO Application Software provided will NOT function with a Macintosh Apple or Linux computer UNICO Application Software Software Installation Loading Software to Computer To install the software please close any open programs and disconnect from the Internet if online then follow the instructions below Step 1 Step 2 Step 3 Step 4 Step 5 Step 6 Insert UNICO Application Software CD into the CD drive of your PC If the software can start the Automatic Setup Screen as Figure 1 go to Step 4 If not go to Step 2 Double click My Computer icon of your PC and locate the CD Drive as Figure 2
14. nstallation Success Connecting Computer to Spectrophotometer Step 1 Remove the RS232 Connection Cable double end out of the bag Step 2 Locate the RS 232C port on the back of your PC Connect the female 9 pin small connector one end of the Connection Cable to the male 9 pin of your PC and secure with the built in screws If your PC does not have a ONLY USE The 25 pin connector if your PC does not have a male 9 pin UNICO Application Software male 9 pin connection use the male 25 pin connector connector common for older upgraded computers Step 3 The 1100RS Spectrophotometer has a male 9 pin RS 232C port on the back panel located to its left side Connect the other end of the connection Step 4 Step 5 Step 6 Step 7 cable to your 1100RS and secure tightly Turn on your PC if not already on and your 1100RS if not already on and let it warm up for fifteen minutes Click the Start button on your PC scroll to Programs UNICO Application Software and locate the VIS1100RS Figure 10 and click it The Start up Screen of VIS1100RS will appear as Figure 11 shown Place the Reference test tube etc in the Sample Compartment push the ENT key on the 110085 panel select the proper port and then click on your Computer Screen The VIS1100RS wil initialize and go to the main screen Figure 12 pre E update EA Whats intern
15. on for each sample to be measured Absorbance Transmittance and the Concentration of the samples will be displayed in the Data Table Figure 27 Concentration Factor Mode The purpose of this test is to measure the Concentration of samples with known multiplication factor to calculate the Concentration Repeat Step 1 to 3 in Absorbance Transmittance Mode section Step 4B Type in the desired Factor in the Text Field at the right of the Factor label Step 5B For each sample be sure to place the sample in Sample Compartment and close the lid Click the TEST Button to record Results The Absorbance Transmittance and Concentration will be displayed in the Data Table Figure 28 17 UNICO Application Software Figure 26 Standard Solution Absorbance Measurement Figure 27 Sample Test Results of Concentration Standard Mode 18 UNICO Application Software S pe za aa slaic Wesen us PME e 9 d mazare nenea Figure 28 Sample Test Results of Concentration Standard Mode Standard Curve The Standard Curve Calibration Curve method allows the operator to Measure up to 8 standards Calculate standard curves with 4 curve fits including 1 Linear Thru Zero set the y intercept equal to zero therefore the curve is forced through zero Calculate and display the slope and Correlation Coefficient 2 Linear Squares Linear
16. rans labels Figure 16 click S button to reset them and unlock the screen Basic Operation Three Analytical Methods Absorbance Transmittance Concentration Standard Curve and Absorbance vs Time Kinetics are illustrated below Absorbance Transmittance Concentration The Absorbance Transmittance Concentration method has the following three modes of operation gt Absorbance Transmittance gt Concentration Standard gt Concentration Factor 13 UNICO Application Software Main Screen of A Trans Conc At the Main Screen Figure 13 click Application Drop down Menu and click A Trans Conc Figure 15 to enter the Main Screen of A Trans Conc Figure 20 ee EE pm olimla alai cs p Rer a rest p Figure 20 Main Screen of A Trans Conc The A Trans Conc Analytical Method has five operation functions Test Wavelength Text Field Test Parameter Test Field TEST Button Data Table and Test Date Test Wavelength Text Field select the desired wavelength by moving the dial on the spectrophotometer and clicking 221 button on the PC screen click OK button Figure 21 at the right of button to set the wavelength Figure 22 The wavelength will be shown both in the Text Field below Current WL nm label and Text Field at the right of the Test Wavelength label Test Parameter Text Field
17. regression model Calculate and display the slope y intercept and Correlation Coefficient for the given standards 2 Order second derivative of the Linear Squares model Calculate and display the coefficients This method is used for non linear standard curves or curves with a Correlation Coefficient of less than 0 9 4 Segmented straight line Use the standards as nodes to connect each point No data is displayed or calculated e Select and view existing standard curves Calculate the Concentrations of unknown samples Main Screen of Standard Curve At the Main Screen Figure 13 click Application Drop down Menu and click Standard Curve Figure 15 to enter the Main Screen of Standard Curve Figure 29 UNICO Application Software olimla gie ore RIZ vene cure EM Figure 29 Standard Curve Setup The following are the basic operations Step 1 Step 2 Step 3 Step 4 Step 5 Insert reference cuvette into the Sample Compartment and close the lid Select the desired wavelength by moving the WAVELENGTH dial on the 1100RS and click zz button on the PC screen to match the desired wavelength click the OK button at the right of button to set the wavelength Click on 0A 100 T Icon Button button the blue cuvette icon to blank the reference Under Standard Sample Setting click the Arrow Button and set
18. the number of Samples standards 2 to 8 Figure 30 Place the standards in the Sample Compartment in order of lowest concentration to highest concentration Type the concentration of the standards e g 0 14 here into the Text Field starting below Cone label and at the right of ID 01 as Figure 31 shown Press Enter key on your computer keyboard or click with the mouse to move to the next cell Repeat the same operation until all concentrations of the standards have been entered 20 UNICO Application Software Step 6 Measure the Absorbance of each standard For each measurement insert the standard into Sample Compartment and click Test button The Absorbance and Transmittance values will be recorded into the Data Table then double click in the appropriate Absorbance cell Abs in the left table next to the standard Conc to get the Absorbance for curve drawing Continue until all of the standards have been measured Figure 31 Alternatively if you do not want the Absorbance of the standards to be recorded into the Data Table you may select the appropriate Absorbance cell Abs in the left table next to the standard Cone and double click the mouse to get the Absorbance results Step 7 Press the Define Curve Button Figure 31 to graph the Standard Curve e g WaterS 1 to WaterS 6 are the standards Figure 32 You may save it for future use Standard sample Setting Sx 5 ls d Figur
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