Fisherbrand II Single Channel User Manual 2002
Contents
1. Finnpipette l is made of mechanically durable and chemically resistant materials PIPETTE OPERATION SETTING THE DELIVERY VOLUME the delivery volume using the push button on top of the pipette To increase the delivery volume turn the push button counterclockwise To decrease the delivery volume turn it clockwise 2 Make sure that the desired delivery volume clicks into place and that the digits are completely visible in the display window Do not set volumes outside the pipette s specified volume range Using excessive force to turn the push button outside the range may jam the mechanism and eventually damage the pipette TIPEJECTION To help eliminate the risk of contamination each pipette is fitted with a tip ejectorsystem The tip ejector system consists of a soft touch tip ejector and specially designed gearing mechanism To release the tip point the pipette at suitable waste receptacle and press the tip ejector with your thumb SAFETY LABEL You can mark the pipette application your initials the calibration date etc on the safety label Remove the clear plastic window on the edge closest to the push button use the service tool that comes with the pipette or a screwdriver Mark the adhesive label with a felt tipped or other pen and snap the window back in place SHELF HANGER En You can attach the pipette shelf hanger on a counter pipette stand or anywhere where you want to hang your pipette Cl2. 0029 1 0030 1 0030 21 0028 1 0029 0030 1 0030 1 0031 1 0031 Bi 0030 1 0030 1 0031 1 0031 0032 1 0032 22 0031 0031 1 0032 1 0032 0033 1 0033 2215 0032 1 0032 0033 1 0033 1 0034 1 0035 23 0033 1 0033 1 0034 1 0035 1 0035 1 0036 2810 0034 1 0035 1 0035 1 0036 1 0036 1 0037 24 0035 1 0036 1 0036 1 0037 0038 1 0038 24 5 0037 1 0037 1 0038 1 0038 1 0039 1 0039 25 0038 1 0038 0039 1 0039 1 0040 1 0041 2515 0039 1 0040 1 0040 1 0041 0041 1 0042 26 0040 1 0041 0042 1 0042 0043 1 0043 26 5 0042 1 0042 0043 1 0043 1 0044 1 0045 27 0043 1 0044 1 0044 1 0045 1 0045 1 0046 215 0044 1 0045 0046 1 0046 1 0047 1 0047 28 0046 1 0046 0047 1 0048 1 0048 1 0049 28 5 0047 1 0048 0048 1 0049 1 0050 1 0050 29 0049 1 0049 0050 1 0050 1 0051 1 0052 235 0050 1 0051 1 0051 1 0052 0052 1 0053 30 0052 1 0052 1 0053 1 0053 1 0054 1 0055 Fisher Scientific For customer service call 1 800 766 7000 To fax an order use 1 800 926 1166 To order online www fishersci com 2002 Fisher Scientific Litho in U S A BN 1507910 02
3. 2 ul mg at 22 C and 95 kPa See the conversion table on page 15 Accuracy systematic error Accuracy is the difference between the dispensed volume and the selected volume of a pipette A V V A accuracy V mean volume V nominal volume Accuracy can be expressed as a relative value 100 x A V Precision random error Precision refers to the repeatability of the pipettings It is expressed as standard deviation s or coefficient of variation cv _py S standards deviation Hi mean volume n 1 n number of measurements Standard deviation can be expressed as a relative value CV CV 100 x S V MAINTENANCE When the Fisherbrand Finnpipette Il is not in use make sure it is Stored in an upright position We recommend a Finnpipette stand for this purpose SHORT TERM CHECKING The pipette should be checked at the beginning of each day for dust and dirt on the outside surfaces of the pipette Particular attention should be paid to the tip cone No other solvents except 70 96 ethanol should be used to clean the pipette LONG TERM MAINTENANCE If the pipette is used daily it should be checked every three months The servicing procedure starts with the disassembly of the pipette DISASSEMBLING 0 2 50 ul PIPETTES lt Won gi 1 Press the tip ejector 2 Insert the maintenance pliers under the ejector bar to release tl tip ejector 3 Remove the tip cone by pressing with maintenance pl
4. 20 12 0080 80 into the openings of the 2 20 ul 20 020 10 010 05 calibration nut at the 2 0 20 100 0 10 50 top of the handle 5 50 ul 50 050 10 020 04 2 Turn the service tool 5 050 10 020 40 10 100 ul 100 0 80 08 0 30 03 clockwise to increase 10 080 80 030 30 or counterclockwise to z0 20041 200 160 08 060 03 decrease the volume 20 160 80 060 30 3 After adjustment check 200 1000 1000 80 08 30 03 the calibration according 200 80 40 30 105 to the instructions above 100 1000 1000 80 08 30 03 100 8 0 8 0 3 0 3 0 FORMULAS FOR 1 5 ml 5000 400 08 150 03 1000 400 40 160 15 CALCULATING 2 10 ml 10000 600 06 300 03 RESULTS 2000 600 30 300 15 Conversion of mass to volume V wte xZ V volume ul w weight mg e evaporation loss mg Z conversion factor for mg ul conversion Evaporation loss can be significant with low volumes To determine mass loss dispense water to the weighing vessel note the reading and start a stopwatch See how much the reading decreases during 30 seconds e g 6 mg 0 2 mg s Compare this to the pipetting time from taring to reading Typically pipetting time might be 10 seconds and the mass loss is 2 mg 10 s x 0 2 mg s in this example If an evaporation trap or lid on the vessel is used the correction of evaporation is usually unnecessary The factor Z is for converting the weight of the water to volume at test temperature and pressure typical value is 1 003
5. 2520 18 b 1033110 19 1131800 14 10593670 9 10593360 20 10593360 d 2207400 20 1132120 21 1090170 1 5ml 10 10593620 0592540 12 10993110 95 1001 6 2207440 13 2206600 5 10592500 10 10593680 14 1131810 6 2207370 11 2206720 15 10593340 0 10593090 12 1030230 16 1030510 1 10593630 13 10593130 2 10593110 14 10593690 5 50 1 2 10593120 15 1058180 16 1131940 5 10592510 bi 6 2207390 CE m 100 1000 10 10593430 de 10293330 200 100011 11 10593630 12 10293310 12 10593110 5 10592530 13 2206430 8 1030170 6 2207420 100ul 14 1131810 9 1030060 6 2207430 200 15 10593340 20 10593290 10 10593410 16 10593330 21 131800 11 10592630 17 1030500 22 0593360 12 10593100 18 1033060 23 1030170 13 10589450 19 10593500 14 1130560 20 1132000 15 1130550 Shelf hanger 16 1054260 17 1030020 Temperature Air pressure hPA mbar 800 853 907 960 013 1067 15 0018 1 0018 1 0019 1 0019 1 0020 1 0020 155 0018 1 0018 1 0019 1 0020 1 0020 1 0021 16 0019 1 0020 0020 1 0021 0021 1 0022 16 5 0020 1 0020 1 0021 1 0022 0022 1 0023 17 0021 0021 0022 1 0022 0023 1 0023 175 0022 1 0022 0023 1 0023 1 0024 1 0024 18 0022 1 0023 1 0024 1 0024 1 0025 1 0025 18 5 0023 1 0024 0025 1 0025 1 0026 1 0026 19 0024 1 0025 0025 1 0026 1 0027 1 0027 195 0025 1 0026 0026 1 0027 0028 1 0028 20 0026 1 0027 0027 1 0028 1 0029 1 0029 20 5 0027 1 0028 1 0028 1
6. Fisherbrand 9 Finnpipette Digital INSTRUCTIONS FOR USE uS Fisher Scientific 2000 PARK LANE Pittsburgh PA 15275 800 766 7000 CONTENTS PRODUCT DESCRIPTION PIPETTE OPERATION PIPETTING TECHNIQUES CALIBRATION MAINTENANCE TROUBLE SHOOTING PACKAGE SPARE PARTS 12 13 EE OC BA PRODUCT DESCRIPTION The Fisherbrand Finnpipette Il is a manual digital pipette It operates on the air displacement principle i e an air interface and uses detachable disposable tips The adjusted delivery volume is displayed digitally on a readout window on the handle The eleven different models of Fisherbrand Finnpipette Il pipettes cover a volume range from 0 2 ul to 10 ml Fisher Catalog Number Volume Range Tip Size 21377814 0 2 ul 2 ul micro 10 21377815 0 5 ul 10 ul micro 10 21377816 0 5 ul 10 ul 250 Universal 200 Ext 21377817 2 ul 20 ul 250 Universal 300 200 Ext 21377818 5 ul 50 ul 250 Universal 300 200 Ext 21377819 10 ul 100 pl 250 Universal 300 200 Ext 21377820 20 200 ul 250 Universal 300 200 Ext 21377821 100 ul 1000 ul 1000 1000 Ext 21377822 200 ul 1000 ul 1000 21377823 1 ml 5 ml 5 ml 21377824 2 ml 10 ml 10 ml DIGITAL DISPLAY he adjusted delivery volume is clearly indicated in the large digital display on the handle RAW MATERIALS The Fisherbrand
7. ean the area where you plan to attach the shelf hanger Apply two stickers to the underside of the shelf hanger Press the shelf hanger firmly into place on a shelf countertop or pipette stand To use hang the grippy finger rest on the shelf hanger PIPETTING TECHNIQUES Push and release the push button slowly at all Figures 4 7 times particularly when working with high A Ready position viscosity liquids Never allow the push button to snap back ake sure that the tip is firmly attached to the C Second stop tip cone Check for foreign particles in the tip Before you begin your actual pipetting work fill and empty the tip 2 3 times with the solution that you will be pipetting Hold the pipette in an upright position while aspirating liquid The grippy should rest on your index finger Make sure that the tips pipette and solution are at the same temperature FORWARD TECHNIQUE En Fill a clean reagent reservoir with the liquid to be dispensed 1 Depress the push button to the first stop 2 Dip the tip under the surface of the liquid in the reservoir to a depth of about 1 cm and slowly release the push button Withdraw the tip from the liquid touching it against the edge of the reservoir to remove excess liquid 3 Deliver the liquid by gently depressing the push button to the first stop After a delay of about one second continue to depress the push button all the way to the second stop This act
8. he pipette Volume range readable graduation under 10 ul 0 00 1 mg 10 100 0 01 mg above 100 ul 0 1 mg Test liquid Water distilled or deionized grade 3 water conforming ISO 3696 Tests are done in a draft free room at a constant 0 5 C temperature of water pipette and air between 15 C to 30 C The relative humidity must be above 50 Especially with volumes under 50 the air humidity should be as high as possible to reduce the effect of evaporation loss Special accessories such as the evaporation trap are recommended PROCEDURE TO CHECK CALIBRATION The pipette is checked with the maximum volume nominal volume and with the minimum volume A new tip is first pre wetted 3 5 times and a series of ten pipettings is done with both volumes A pipette is always adjusted for delivery Ex of the selected volume Procedure 1 Do 10 pipettings with the minimum volume 2 Do 10 pipettings with the maximum volume 3 Calculate the accuracy A and precision cv of both series 4 Compare the results to the limits in the Table 1 If the calculated results are within the selected limits the adjustment of the pipette is correct TABLE 1 Maximum permissible errors according 1508655 Range Volume Accuracy Precision Ho d w Adjustment is done with 0 2 2 ul 2 0 080 4 0 040 2 0 the service tool 02 0080 40 0 020 200 0 5 10ul 10 0 120 1 2 0 080 0 8 1 Place the service tool 1 01
9. iers 4 Pull out the piston and the spring 5 Keep the tip cone vertically and push out with piston the rest of the piston assembly Then keep the tip cone upside down and tap all parts from tip cone Remember keep all parts in order on table for reassembly 6 Clean the piston the piston spring and the O rings with a dry napless cloth Check the tip cone for foreign particles Grease the cleaned parts with the lubricant that comes with the pipette Reassemble the pipette components All 0 2 50 ul First slide the spring 14 spring support 15 and Es 16 back on the piston Compress the spring with fingers by pressi piston and spring support 15 agains each other 5 50 ul Slide bigger O ring 17 smaller O ring 18 2 20ul bigger O ring 18a smaller O ring 18b spring support 19 sharp edges against spring and small spring 20 on the piston EO 9 0 5 10 ul First slide O ring tube 17 larger hole first bigger O ring 18 smaller O ring 19 and O ring support 20 on the piston Then slide small spring 21 spring support 22 sharp edges against spring and O ring 22 on the O ring support 20 0 2 2 ul First slide O ring tube 17 larger hole first and sealing combination 18 on the piston Then slide small spring 19 spring support 20 sharp edges against spring and O ring 21 on the sealing combination 18 All 0 2 50 pl Carefully slide the entire assembly into the tip cone and release your fingers 10 With the push butto
10. ion will empty the tip 4 Release the push button to the ready position If necessary change the tip and continue pipetting REVERSE TECHNIQUE The reverse technique is suitable for dispensing liquids that have a high viscosity or a tendency to foam easily The technique is also recommended for dispensing very small volumes Fill a clean reagent reservoir with the liquid to be dispensed 1 2 4 REPETITIVE TECHNIQUE Depress the push button all the way to the second stop Dip the tip under the surface of the liquid in the reservoir to a depth of about 1 cm and slowly release the push button This action will fill the tip Withdraw the tip from the liquid touching it against the edge of the reservoir to remove excess liquid Deliver the preset volume by gently depressing the push button to the first stop Hold the push button at the first stop Some liquid will remain in the tip and this should not be included in the delivery The remaining liquid should either be discarded with the tip or pipetted back into the container The repetitive technique offers a rapid and simple procedure for repeated delivery of the same volume Fill a clean reagent reservoir with the liquid to be dispensed 1 2 Depress the push button all the way to the second stop Dip the tip under the surface of the liquid in the reservoir to a depth of about 1 cm and slowly release the push button This action will fill the tip Withdraw the tipf
11. is designed to allow easy in lab service If you would prefer to have us or your local representative service your pipette please make sure that the pipette has been decontaminated before you send it to us Please note that the postal authorities in your country may prohibit or restrict the shipment of contaminated material by mail 0 2 2ul 0 5 10p1 5 50pl 100 1000yl 20 200p1 Ce MINI qu e nm 3 e N e a E GENE 0 e N 0 2 e 2 0 2 am 23 e e lt Ge 8 0 CD t To S o e N e e Y 10 10 12 17 18 19 20 21 2 E 15 13 SPARE PARTS 15 lists spare parts and reorder numbers a 20 20011 2 20yl 0 2 2 0593720 5 10592520 5 10592510 5 10592560 2 2900580 6 2207410 6 2207380 6 2207360 3 10593050 10 10593420 10 10593090 10 10593090 4 1527200 11 10593630 11 10593630 11 10593630 2 ni 12 10593110 12 10593110 12 10593120 13 1053840 3 2206440 13 2205700 0592550 14 1130510 4 1131810 14 1131810 3 2207450 15 1053860 5 10593340 15 10593340 10 10593660 16 1030160 6 10593330 16 10593330 11 2206730 7 10593320 17 10593300 12 1033050 10 1001 8 1030380 18 2205730 13 10593440 5 1059
12. n depressed all the way carefully attach the tip cone to the handle so that the adapter opening is on the tip ejector side Do not bend the thin piston wire when assembling Press in the snap joints 11 Assemble the tip ejector and check the calibration according to the instructions DISASSEMBLING 50 1000 ul PIPETTES ress the tip ejector insert the pliers under the ejector bar to release the tip ejector Remove the tip cone using the maintenance pliers Pull out the piston Remove the O ring O ring support and spring from the tip cone Clean the piston the piston spring and the O ring with a dry napless cloth Check the cylinder for foreign particles Grease the cleaned parts with the lubricant that comes with the pipette Slide parts over the piston pressing down the large spring Attach the tip cone to the handle so that the adapter opening is on the tip ejector side and press in the snap joints 10 Check the calibration according to the instructions DISASSEMBLING 1 10 ml PIPETTES ess the tip ejector ert the pliers under the ejector bar to release the tip ejector Remove the part 2 from part 1 of the tip ejector using the maintenance pliers to release the snap joint Remove the cylinder by pressing part 1 of the tip ejector firmly owards the cylin der This action releases the snap joint so you can remove the cylinder 5 Clean the O ring and cylinder Regrease the O ring and the cylinder 6 Assemble the parts in
13. rom the liquid touching against the edge of the reservoir to remove excess liquid Deliver the preset volume by gently depressing the push button to the first stop Hold the push button at the first stop Some liquid will remain in the tip and this should not be included in the delivery 4 Continue pipetting by repeating steps 2 and 3 7 BIBiPETTING WHOLE BLOOD deproteinization in blood glucose determination for example Use steps 1 and 2 of the forward technique to fill the tip with blood Wipe the tip carefully with a dry clean tissue 1 2 Immerse the tip into the reagent depress the push button to the first stop making sure the tip is well below the surface Release the push button slowly to the ready position This will fill the tip Keep the tip in the solution 3 Depress the push button to the first stop and release slowly Keep repeating this procedure until the interior wall of the tip is clear 4 Finally depress the push button all the way to the second stop to completely empty the tip CALIBRATION All Finnpipettes are factory calibrated and adjusted to give the volumes as specified with distilled or deionized water The pipettes are constructed to permit re adjustment for liquids of different temperature and viscosity DEVICE REQUIREMENTS AND TEST CONDITIONS An analytical balance must be used The scale graduation value of the balance should be chosen according to the selected test volume of t
14. the opposite order of disassembly All joints are snap fit and can be pushed together by hand Be careful not to bend the pipette during assembly because this could damage the snap joints or the piston 7 Check the calibration according to the instructions 5 6 7 8 9 10 TROUBLE SHOOTING The table below lists possible problems and their solutions Defect Possible reason Solution Leakage Tip incorrectly attached Attach firmly Foreign particles Clean tip cones attach between tip and tip cone new tips Foreign particles between the Clean and grease O ring piston the O ring and the and cylinder cylinder Insufficient amount of grease Grease accordingly on cylinder and O ring O ring damaged Change the O ring Inaccurate Incorrect operation Follow instructions carefully dispensing incorrectly attached Attach firmly Calibration altered caused Recalibrate according to by misuse for example instructions Inaccurate Unsuitable calibration Recalibrate with dispensing High viscosity liquids may the liquids in question with certain require recalibration liquids PACKAGE The Fisherbrand Finnpipette Il is shipped in a specially designed package containing the following items 1 The Finnpipette 4 Tube of grease 7 Shelf hanger 2 Service tool 5 Instruction manual 8 Two stickers 3 Maintenance pliers 6 Calibration certificate CAUTION The Fisherbrand Finnpipette 1
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