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1. 24 ADA 9904000 9904300 24 ADA w Self Closing Doors 18 ADA 9904100 9904400 12 ADA 9904200 9904500 3 Base Stands and Mobile Stands 3746702 4 Telescoping Base Stand with Fixed Feet 48 w x 29 25 d x 27 5 to 33 5 h 3746712 4 Telescoping Base Stand with 5 Casters 48 w x 29 25 d x 27 5 to 33 5 h 18 Product Service 1 800 522 7658 CHAPTER 8 TROUBLESHOOTING Refer to the following table if your enclosure fails to operate properly If the suggested corrective actions do not solve your problem contact Labconco for additional assistance PROBLEM Lights do not work CAUSE CORRECTIVE ACTION Circuit breaker s Reset or replace circuit breaker tripped Lamp not installed properly Inspect lamp installation Lamp Wiring disconnected Inspect lamp wiring Defective lamp Replace lamp Light switch is defective Replace light switch UV light timer does not work PCR only No power Power cable to UV light timer circuit board is disconnected No lights on Verify connection No display Replace circuit board for UV light timer if it malfunctions Product Service 1 800 522 7658 19 20 APPENDIX A REPLACEMENT PARTS The following illustrations indicate the replacement parts Product Service 1 800 522 7658 Appendix A R
2. N O 10 10 12 14 15 15 15 16 CHAPTER 7 ACCESSORIZING YOUR ENCLOSURE Work Surfaces Storage Cabinets Base Stands amp Mobile Stands CHAPTER 8 TROUBLESHOOTING APPENDIX A REPLACEMENT PARTS APPENDIX B DIMENSIONS APPENDIX C SPECIFICATIONS DECLARATION OF CONFORMITY 17 17 18 18 19 20 23 24 27 CHAPTER 1 INTRODUCTION Congratulations on your purchase of a Labconco Purifier Passive PCR Enclosure or Purifier Tissue Culture Enclosure Your non ventilated enclosure is designed to reduce cross contamination in laboratory applications The Purifier Passive PCR Enclosure and Purifier Tissue Culture Enclosure offer a UV light for disinfecting the interior and an interior switched electrical outlet The Purifier Passive PCR Enclosure UV light has the added feature of a digital timer to accommodate PCR protocol To take full advantage of all the features please acquaint yourself with this manual and keep it handy for future reference Polymerase Chain Reaction Definition PCR Polymerase Chain Reaction PCR is a laboratory process in which a particular DNA segment from a mixture of DNA chains is rapidly replicated producing a large readily analyzed sample of a piece of DNA In PCR DNA is immersed in a solution containing the enzyme DNA polymerase unattached nucleotide bases the subunits that DNA is composed of and primers short sequences of nucleotides designed to bind with an end of the desi
3. Avoid direct exposure to ultraviolet UV radiation NEVER work in the non ventilated enclosure with the UV light on Always use the UV sash closure in the closed position when using the UV light to provide added protection Product Service 1 800 522 7658 13 CHAPTER 5 Usinc Your ENCLOSURE Planning e Thoroughly understand procedures and equipment required before beginning work e Arrange for minimal disruptions such as room traffic or entry into the room while the enclosure is in use Start up e Clean and surface disinfect the inside of the enclosure Use UV light as required for protocol e Turn on the fluorescent light e Only raise the sash for loading e Wear a long sleeved lab coat and rubber gloves Use protective eyewear Wear a protective mask if appropriate Consult your Safety Officer for additional personal protective equipment recommendations Work Techniques e Keep all materials inside and perform all operations inside the work area Unloading Materials and Equipment e All open trays weigh vessels and containers should be covered before being removed from the enclosure Shutdown e Turn off the fluorescent light and close the UV sash closure e Clean and surface disinfect the interior surfaces after completion e Turn on the UV light to decontaminate the work area as determined by your protocol 14 Product Service 1 800 522 7658 CHAPTER D MAINTAINING YOUR ENCLOSURE Now that you
4. Outlet Power 19B 1 1306000 Power Cord Outlet Power 20A 1 1230700 Receptacle Interior Switched Outlet 115V 20B 1 1294400 Receptacle Interior Switched Outlet 230V 21A 1 1327201 Circuit Breaker 10 Amp 115V 21B 2 1327204 Circuit Breaker 5 Amp 230V 22 8 1591606 Rubber Bumper UV Sash Closure Product Service 1 800 522 7658 21 Appendix A Replacement Parts te a i3 oe Light 3 Pos 20 Interior Figure A 2 22 Product Service 1 800 522 7658 Appenpix B Dimensions See the following dimensions Product Service 1 800 522 7658 23 Appenpix C SPECIFICATIONS This appendix contains electrical specifications environmental conditions and wiring diagrams Electrical Specifications e 10 Amps 115V 60 Hz or 5 Amps 230V 50 60 Hz Purifier Passive PCR Enclosure or Purifier Tissue Culture Enclosure The auxiliary outlet load is 8 Amps on 115V and 4 Amps on 230V Environmental Conditions Indoor use only Maximum altitude 6562 feet 2000 meters Ambient temperature range 41 to 104 F 5 to 40 C Maximum relative humidity 80 for temperatures up to 88 F 31 C decreasing linearly to 50 relative humidity at 104 F 40 C e Main supply voltage fluctuations not to exceed 10 of the nominal voltage e Transient over voltages according to Installation Categories II Over voltage Categories per IEC 1010 Temporary voltage spikes on the AC input lin
5. have an understanding of how to work in the non ventilated enclosure we will review the suggested maintenance schedule and common service operations Routine Maintenance Schedule Weekly e Clean the enclosure interior appropriate for the application Monthly or more often as reguired e Using a cloth and glass cleaner clean the exterior surfaces of the non ventilated enclosure particularly the front to remove any accumulated dust e All weekly activities Annually e Replace the UV lamp e All monthly activities UV Light Replacement rd 1 Disconnect power 2 Locate the UV lamp inside 3 Rotate and remove the old UV lamp 4 Reinstall the new UV lamp 5 Switch unit on and try the new UV lamp Product Service 1 800 522 7658 15 Chapter 6 Maintaining Your Enclosure Fluorescent Light Replacement 1 2 3 4 Disconnect power Locate the two Phillips machine screws on top of the light and remove Lift up the light assembly Rotate and remove the old fluorescent lamp Reinstall the new fluorescent lamp light and screws in reverse order Switch unit on and try the new fluorescent lamp Product Service 1 800 522 7658 CHAPTER 7 ACCESSORIZING YOUR ENCLOSURE There are several ways to accessorize the enclosure for your individual requirements These include the addition of accessory work surfaces storage cabinets base stands and mobile stands 1 Work Surfaces An optional dished
6. work surface is available Dished work surfaces are contoured to fit the enclosure to contain spills Epoxy is chemical resistant Catalog Description Dimensions W x D x H 3908402 Black 4 foot wide 48 x 26 66 x 1 3908405 Gray 4 foot wide 48 x 26 66 x 1 Product Service 1 800 522 7658 17 Chapter 7 Accessorizing Your Non Ventilated Enclosure 2 Storage Cabinets SOLVENT ACID 48 9902000 9901000 36 9902100 9901100 30 9902200 9901200 24 9902300 9902400 9901300 9901500 18 9901400 9901600 12 48 w Self Closing Doors 9903000 36 w Self Closing Doors 9903100 30 w Self Closing Doors 9903200 24 w Self Closing Doors 9903300 9903400 24 ADA 9906000 9906100 9905000 9905200 24 ADA w Self Closing 9906200 9906300 Doors 18 ADA 9905100 9905300 12 ADA STANDARD BASE VACUUM PUMP Size Description Dual Doors Right Hinge Left Hinge Dual Doors Right Hinge Left Hinge 48 9900000 36 9900100 30 9900200 24 9900300 9900600 18 9900400 9900700 9907000 9907100 12 9900500 9900800 48 w Self Closing Doors 36 w Self Closing Doors 30 w Self Closing Doors 24 w Self Closing Doors
7. User s Manual Purifier Non Ventilated PCR amp Tissue Culture Enclosures Models 3951400 3951420 3952400 3952420 Register your product online at www labconco com productreg html gt 4 or return the attached card and E receive FREE LabbyWear Labconco s Mascot Labby the LABster Protecting your laboratory environment L gt Z LABCONCO LABCONCO CORPORATION 8811 PROSPECT AVENUE KANSAS CITY MO 64132 2696 800 821 5525 816 333 8811 FAX 816 363 0130 E MAIL labconco labconco com HOME PAGE www labconco com Copyright Information Copyright O 2004 Labconco Corporation All rights reserved Labconco Corporation 8811 Prospect Avenue Kansas City Missouri 64132 The information contained in this manual and the accompanying product is copyrighted and all rights are reserved by Labconco Corporation Labconco Corporation reserves the right to make periodic design changes without obligation to notify any person or entity of such change Purifier Passive PCR Enclosure and Purifier Tissue Culture Enclosure Warranty Labconco provides a warranty on all parts and factory workmanship The warranty includes areas of defective material and workmanship provided such defect results from normal and proper use of the equipment The warranty for all Labconco products will expire one year from date of installation or two years from date of shipment from Labconco whichever is sooner except the following e Pur
8. __ Appearance __ Reputation __ Service Program __ Performance Specifications __ Ease of Operation __ Safety Features __ Price __ Dealer Recommendation __ Availability __ Colleague Recommendation _ __ Other please specify From whom did you purchase your enclosure DEALER Please fold card over tape edges and mail No postage is required AD dei dn HL NON VENTILATED ENCLOSURES 4Z LABCONCO 800 821 5525 or 816 333 8811 Fax 816 363 0130 REV 09 04 LabbyWear is the official uniform of LABsters everywhere Be a LABster As a token of our thanks for returning your Product Registration Card we would like to send you the LabbyWear of your choice Please select from one of the three items listed or collect Labby Bucks and redeem for other LabbyWear merchandise Then just complete the information reguested on the reverse side fold tape edges and mail O T shirt 100 cotton short sleeved white with Labby The LABster embroidered on the front left side Specify unisex size A Large A XLarge IS Twill cap Pigment dyed two color with six panels and Labby The LABster embroidered on the front Q Lunch bag Insulated teal with royal blue accent and Labby The LABster embroidered on the front Q 10 Labby Bucks and LabbyWear Catalog RL L266 cELV9 OIN ALIO SVSNV ANNAAV LOAdSOUd 1188 NOLLIVHOdHOO OONOOGgV 1 HE H pg 2295520 00Y Ad GlVd 39 TIM ADVLSOd I OW ALIOSVSNVM 99lFON LllNH3
9. be nearby for connecting the Purifier PCR or Purifier Tissue Culture Enclosures Please review Chapter 2 for electrical prereguisites All wiring for the building electrical outlets SHOULD be performed by a licensed electrician and conform to all local codes Sealing the Enclosure to the Work Surface When the enclosure has been set in place it may be sealed at the work surface to prevent spilled materials from collecting under the walls A bead of silicone sealant is recommended to seal it to the work surface Product Service 1 800 522 7658 9 CHAPTER 4 FEATURES AND SAFETY PRECAUTIONS Features Labconco engineered the Purifier Passive PCR Enclosure and Purifier Tissue Culture Enclosure to provide a non ventilated circulation free work area to reduce cross contamination in laboratory applications A spacious interior and convenience outlet give ample room for media preparation and eguipment used for procedures A UV light with digital timer included on the Purifier PCR Enclosure provides decontamination control after PCR protocols are conducted The Purifier Tissue Culture Enclosure may be used for technigues such as tissue staining culture growth and virus harvesting A built in UV light on the Purifier Tissue Culture Enclosure provides secondary surface decontamination while the enclosure is not in use Product Service 1 800 522 7658 Chapter 4 Features and Safety Precautions Figure 4 1 Features 1 E
10. ces to secure the enclosure Product Service 1 800 522 7658 7 Chapter 3 Getting Started MM Work Surface Specifications The work surface should be smooth rigid and durable such as a chemically resistant epoxy resin The surface should be non porous and resistant to the materials used in conjunction with the Purifier PCR or Purifier Tissue Culture Enclosures The work surface should also contain a dished recessed area for containing primary spills Work Surface and Non Ventilated Enclosure Installation 1 Level the base cabinets and the work surface Work surface should be placed flush with the front of the base cabinet as shown in Figure 3 1 2 Position the work surface in its intended location and with the front of the work surface wide flange towards you 3 Secure the work surface to the base cabinet with a structural adhesive or silicone sealant 4 Insert the supplied mounting screws in the four holes Allow a minimum of 1 8 clearance under the head of the screw for positioning the enclosure 5 Place the enclosure on the work surface and slide the rear flange and lower front bracket flanges under the mounting screw heads 6 Tighten the four screws to complete the installation FEM RCR A OFTE EP L Ho Figure 3 1 Side View of Typical Installation 8 Product Service 1 800 522 7658 Chapter 3 Getting Started Connecting the Electrical Supply A grounded receptacle should
11. ch you are engaged Q Quality Control Q Teaching lnstructional O Testing O Other please specify Q Clinical Diagnostic O Research amp Development Q Production Of what type organization is your work a part Q Industrial Manufacturing Utility Q Government except Medical Q Educational Institution except Medical School Q Independent Foundation Consulting Q Medical Medical School Hospital Public Health QO Pharmaceutical Research Q Other please specify Which comes closest to describing your scientific discipline Q Biological Science Q Inorganic Chemistry O Physical Chemistry Q Bio Chemistry O Analytical Chemistry R amp D O Engineering or Physics Q Polymer Chemistry Q Analytical Chemistry Quality Control Q Other please specify Q Organic Chemistry Which product did you purchase Q Purifier PCR Enclosure Q Purifier Tissue Culture Enclosure Model Number Serial Number Date of Installation Model and serial numbers are located on the top and on the back Indicate Labconco accessories purchased with your enclosure O Work Surface O Other Q Base Stand Q Base Cabinet How did you learn about the enclosure O Dealer Sales Rep O Dealer Catalog Q Colleague O Advertisement Q Labconco Sales Rep O Labconco Literature Trade Show Olnternet WWW Q Other please specify What factors most influenced your decision to purchase the enclosure Number up to 3 1 being the most important
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13. e that may be as high as 1500V for 115V models and 2500V for 230V models are allowed e Used in an environment of Pollution degrees 2 i e where normally only non conductive atmospheres are present Occasionally however a temporary conductivity caused by condensation must be expected in accordance with IEC 664 24 Product Service 1 800 522 7658 Appendix C Specifications WAIN CHITAC ad T OF aci 16901541 Boas ou sm MT NYMHOYIO 2NISIM 09N098gV An SHAS GL Yee Janis Mwd AGHI LOMMDOSE NING YAN BEDDAU n d DELA 91345 Im nen ieri Wn WE HD nL Mid WLAD 15 FRM LER Se 1 594 LA HEL UA I Er rt ir DOC da ____ l DEI Mid i vyti RESHMI a ADtv 301 a NULS L 574908 LINO WHEL 25 Product Service 1 800 522 7658 Appendix C Specifications YAS Dhire 8 4 Te 60 06 ADE TOTS Wd SL ee HOLIIICHA WWM2WIO DNISIM O9NO09O8V 1 TT TTS TE UL Hadd 338005 HWD Med 125390190 NING Y M E Deli Aya y p b p p b b E 4 B LI B B E E B E E L ga Tar A ym i interer dla i oa asa itbat def L F Kera Brata MMOD cos KL THO MOL WE STICA TR 9 ED1FDA EOW Bou b U STAL L INEST EU YR OAT AD DW 13150 WARES Product Service 1 800 522 7658 26 DECLARATION OF CONFORMITY Application Council Directiwe s TNINWEEC 89 336 EEC Standards to which conformity is declared EN61010 EN55022 EN50082 1 Manufacturer s Name Labconco Corporatio
14. eplacement Parts Item Qty Part Number Description 1 1 3906500 Glass Side Short 2 1 3906102 Glass Sash 4 Short 3 1 3905602 Lower Foil 4 4 2 3 Ft 6913700 Wiper Sash 5A 1 1927403 Spring Compression Sash Latch 5B 1 3906700 Latch Bracket 5C 1 6916500 Latch Sash 5D 2 1893206 Screw 8 32 x 38 Phil SS Type F 6 1 3945200 Sash Assembly 4 7A 1 3907400 Bracket Air Foil Right 7B 1 3907401 Bracket Air Foil Left 7C 2 1932401 Washer Shoulder Plastic 7D 2 1912108 Washer 194 ID x 38 OD x 03 thick plastic 7E 2 1909217 Cap Nut 10 24 SS 8 1 3905302 Header 4 9A 1 1934601 Bushing Heyco with Flex Shutter 1 50 dia 9B 1 1936800 Bushing Heyco Closed 1 50 dia 10A 2 7868402 Spacer Bushing 10B 2 1912108 Washer 194 ID x 38 OD x 03 T Plastic 10C 2 1889316 Screw 10 24 x 1 00 PH SS 11A 1 3929400 Label Tissue Culture Enclosure 11B 1 3929500 Label PCR Enclosure 12 1 3945300 Timer Digital UV PCB 13A 1 1307000 Switch 2 position Aux Outlet 13B 1 1306300 Switch 3 position Light 14 1 9721901 Lamp Fluorescent FT25T8 25 Watt 15 1 1271300 Lamp UV G30T8 30 Watt 16 1 1294000 Ballast Fluorescent 17A 1 1233400 Ballast UV 115V 17B 1 1233600 Ballast UV 230V 18A 2 1333800 Inlet Power IEC 18B 1 1334500 Power Cord 115V 18C 1 1334100 Power Cord 230V 19A 1 1333900 Receptacle
15. he UV light is activated 9 Switched Outlet is located inside the non ventilated enclosure for powering electrical equipment 10 Accessory Work Surface is dished to contain spills and is contoured to fit the dimensions of the non ventilated enclosures Safety Precautions Use good housekeeping in the enclosure at all times Clean up spills immediately Periodically clean all interior surfaces Do not work with hazardous volatile 1 chemicals in this enclosure All acid use in this enclosure is prohibited Radioisotope materials are prohibited in this enclosure 12 Product Service 1 800 522 7658 _ T_ _ ___ F ERR RcE E OE E SFF y VYVyVYE E amp _ Chapter 4 Features and Safety Precautions The use of safety goggles protective clothing gloves and any other personal protective eguipment recommended by your safety officer should be used The sash should remain in the down position while using the enclosure Ensure the enclosure is connected to an electrical service in accordance with local and national electrical codes Failure to do so may create a fire or electrical hazard Do not remove or service any electrical components without first disconnecting from electrical service Ensure only trained operators use the non ventilated enclosure All users should review the User s Manual and become familiar with the operation
16. ifier Delta Series Biological Safety Cabinets and PuriCare Procedure Stations carry a three year warranty from date of installation or four years from date of shipment from Labconco whichever is sooner e FlaskScrubber SteamScubber and FlaskScrubber Vantage Series Glassware Washers carry a two year warranty from date of installation or three years from date of shipment from Labconco e Carts carry a lifetime warranty e Glass is not warranted from breakage due to accident or mishandling This limited warranty covers parts and labor but not transportation and insurance charges In the event of a warranty claim contact Labconco Corporation or the dealer who sold you the product If the cause is determined to be a manufacturing fault the dealer or Labconco Corporation will repair or replace all defective parts to restore the unit to operation Under no circumstances shall Labconco Corporation be liable for indirect consequential or special damages of any kind This statement may be altered by a specific published amendment No individual has authorization to alter the provisions of this warranty policy or its amendments Lamps and filters are not covered by this warranty Damage due to corrosion or accidental breakage is not covered Limitation of Liability The disposal and or emission of substances used in connection with this equipment may be governed by various federal state or local regulations All users of this equipment are re
17. luded Chapter 1 Introduction provides a brief overview of the non ventilated enclosure explains the organization of the manual and defines the typographical conventions used in the manual Chapter 2 Prereguisites explains what you need to do to prepare your site before you install the enclosure Electrical and service reguirements are discussed Chapter 3 Getting Started contains the information you need to properly unpack inspect and install the enclosure Chapter 4 Features and Safety Precautions explains how the enclosure operates and the appropriate precautions you should take when using it Chapter 5 Using Your Enclosure discusses the basic operation of how to prepare use and turn off your non ventilated enclosure is included Chapter 6 Maintaining Your Enclosure explains how to perform routine maintenance Product Service 1 800 522 7658 Chapter 1 Introduction Chapter 7 Accessorizing Your Enclosure explains acceptable modifications or how to add accessories Chapter 8 Troubleshooting contains a table of problems you may encounter while using the product including the probable causes of the problems and suggested corrective actions Appendix A Components contains labeled diagrams of all of the components of the enclosures Appendix B Dimensions show all of the dimensions for the enclosures Appendix C Specifications contains the electrical reguirements Wiring diagrams are also included in this sec
18. n Manufacturera Address 8811 Prospect Avenue Kansas City MO 64132 USA Importer s Name See Shipping Customs Documents Importer s Address See Shipping Customs Documents for your equipment Type of Equipment Purifier PCR Enclosure and Purifier Tissue Culture 393 1400 Series 4 Tissue Calbare Enclosane 39372410 Series Passive PCR Enclosure Serial No Various See Individual Declaration Year of Manufacture 2004 and subsequent I the undersigned hereby declare that the equipment specified above conforms to the above Directive s and Standards See individual Declaration of Conformity which will be signed by the importer for your country Signature Full Name Position An individual version of this declaration is included with your shipping customs documentation Laboonco P N 36960 54 REY SI TTI IRT _ rrr irrew me lt s _ l1t _ Product Service 1 800 522 7658 27 Register Online Go to P RO D U CT R EG ISTRATI O N CAR D www labconco com productreg html By registering your product you will receive these important benefits e Warranty Confirmation e Product Registration e Product Protection e Free LabbyWear Merchandise NAME TITLE DEPARTMENT INSTITUTION ADDRESS CITY STATE ZIP COUNTRY TELEPHONE EXT FAX E MAIL Which of the following comes closest to describing the type of activity in whi
19. ne the location You must be certain that the area is level and of solid construction In addition a dedicated source of electrical power should be located near the installation site to power the enclosure Carefully read this chapter to learn the reguirements for your installation site The support reguirements The location and air current reguirements The electrical power reguirements The space reguirements Support Reguirements At a minimum the supporting structure usually consists of a base cabinet and chemically resistant work surface A mobile stand or cart is allowable See Chapter 7 for accessory work surfaces stands mobile stands and cabinets Location and Air Current Reguirements The Purifier Passive PCR and Purifier Tissue Culture Enclosures have been designed to reduce cross drafts and air circulation with the sash and UV sash closure shut However as a precautionary measure of safety and a higher level of guality management it is recommended that the enclosure be placed in such an area away from Product Service 1 800 522 7658 Chapter 2 Prereguisites e High traffic areas where walking might cause an air disturbance e Overhead or wall HVAC diffusers fans radiators or other lab equipment producing air currents e Next to doorways or windows that may be opened Electrical Requirements Standard duplex electrical receptacles should be nearby for connecting the non ventilated Purifier Passi
20. nterstate Commerce inspect it for damage that may have occurred in transit If Commission rules damaged notify the delivery carrier immediately and retain the MM O be filed with the delivery entire shipment intact for inspection by the carrier amoan ince C5 days of delivery UNAUTHORIZED RETURNS WILL NOT BE ACCEPTED IF ENCLOSURE WAS DAMAGED IN TRANSIT YOU MUST FILE A CLAIM DIRECTLY WITH DO NOT RETURN GOODS WITHOUT THE PRIOR AUTHORIZATION OF LABCONCO THE FREIGHT CARRIER LABCONCO CORPORATION AND ITS DEALERS ARE NOT RESPONSIBLE FOR SHIPPING DAMAGES Do not discard the packing material until you have checked all of the components and tested the enclosure We recommend that you do not remove the enclosure from its shipping container until it is ready to be placed into its final location Move the unit by using a floor jack Do not move the enclosure by tilting it onto a hand truck Installing the Enclosure ona Supporting Structure and Work Surface When installing the enclosure onto a chemically resistant work surface or benchtop ensure that the structure can safely support the combined weight of the enclosure and any related equipment The work surface should be at least as wide and deep as the non ventilated enclosure to properly support it The front of the non ventilated enclosure should be aligned within 1 8 of the front of the work surface Mounting holes are provided in the Labconco accessory work surfa
21. quired to become familiar with any regulations that apply in the user s area concerning the dumping of waste materials in or upon water land or air and to comply with such regulations Labconco Corporation is held harmless with respect to user s compliance with such regulations Contacting Labconco Corporation If you have questions that are not addressed in this manual or if you need technical IS assistance contact Labconco s Customer Service Department or Labconco s Product Service Department at 1 800 821 5525 or 1 816 333 8811 between the hours of 7 00 a m and 6 00 p m Central Standard Time Visit Labconco s web site at http www labconco com or email Labconco at labconco labconco com Part 3939200 ECO B730 TABLE Or CONTENTS CHAPTER 1 INTRODUCTION Polymerase Chain Reaction Definition PCR About This Manual Typographical Conventions CHAPTER 2 PREREQUISITES Support Requirements Location and Air Current Requirements Electrical Requirements Space Requirements CHAPTER 3 GETTING STARTED Unpacking Your Enclosure Installing the Enclosure on a Supporting Structure amp Work Surface Connecting the Electrical Supply Sealing the Enclosure to the Work Surface CHAPTER 4 FEATURES AND SAFETY PRECAUTIONS Features Safety Precautions CHAPTER 5 USING YOUR ENCLOSURE CHAPTER 6 MAINTAINING YOUR ENCLOSURE Routine Maintenance Schedule UV Light Replacement Fluorescent Light Replacement Dn Lh LR E E LM HA e
22. red DNA segment Two primers are used one primer binds at one end of the desired segment on one of the two paired DNA strands and the other primer binds at the other end but on the other strand The solution is heated to break the bonds between the Product Service 1 800 522 7658 Chapter 1 Introduction strands of the DNA When the solution cools the primers bind to the separated strands and DNA polymerase guickly builds a new strand by joining the free nucleotide bases to the primers When this process is repeated a strand that was formed with one primer binds to the other primer resulting in a new strand that is restricted solely to the desired segment Thus the region of DNA between the primers is selectively replicated Further repetitions of the process can produce billions of copies of a small piece of DNA in several hours PCR was developed in 1985 by Kary B Mullis who was awarded the 1993 Nobel Prize in chemistry for his work It is used in a broad range of applications from DNA fingerprinting to medical tests to identify diseases from the infectious agent s DNA See also nucleic acid The Concise Columbia Encyclopedia is licensed from Columbia University Press Copyright O 1995 by Columbia University Press All rights reserved About This Manual This manual is designed to help you learn how to install use and maintain your non ventilated enclosure Instructions for installing optional eguipment on your enclosure are also inc
23. rgonomic Slope and Front of 20 provides maximum visibility and comfort reduces glare thereby minimizing operator fatigue 2 Internal Depth of 23 and interior height of 22 provides necessary depth and height to support procedures and other auxiliary eguipment without extending outside the non ventilated enclosure 3 Flush Sash has a wiping seal and features a spring loaded latch for loading auxiliary equipment The sash pivots down for normal operation 4 Electrical Pass Through Iris allows electrical cords and data cords to pass through the back of enclosure The unit ships with solid plugs and the iris plugs are included with the User s Manual for your convenience Product Service 1 800 522 7658 11 Chapter 4 Features and Safety Precautions 5 Fluorescent Light provides illumination for the interior of the enclosure 6 Ultraviolet Germicidal Light provides surface decontamination and maintains sterility inside the enclosure It is located above the work area 7 Variable PCR Digital UV Light Timer Purifier PCR PCR Enclosure only allows the operator to time the surface disinfection of the work area for PCR protocols The UV light timer has eight settings of 5 10 15 30 60 120 240 minutes and infinitely ON It is easily changed by selecting the time necessary on the control panel 8 UV Sash Closure provides added safety to prevent access and air currents inside the non ventilated enclosure when t
24. tion Typographical Conventions Recognizing the following typographical conventions will help you understand and use this manual Book chapter and section titles are shown in italic type e g Chapter 3 Getting Started e Steps required to perform a task are presented in a numbered format e Comments located in the margins provide suggestions reminders and references e Critical information is presented in boldface type in paragraphs that are preceded by the exclamation icon Failure to comply with the information following an exclamation icon may result in injury to the user or permanent damage to the enclosure e Critical information is presented in boldface type in paragraphs that are preceded by the wrench icon A trained certifier or contractor should only perform these operations Failure to comply with the information following a wrench icon may result in injury to the user or permanent damage to your hood e Important information is presented in capitalized type in paragraphs that are preceded by the pointer icon It is imperative that the information contained in these paragraphs be thoroughly read and understood by the user S Mk e The PCR icon indicates the text is specific to the Purifier PCR PCR Enclosure e The TC icon indicates the text is specific to the Purifier Tissue TC Culture Enclosure Product Service 1 800 522 7658 3 CHAPTER 2 PREREOUISITES Before you install the enclosure carefully exami
25. ve PCR Enclosure or Purifier Tissue Culture Enclosure For your convenience the enclosures have a switched auxiliary outlet receptacle The auxiliary switched outlet is rated for either 115V 8 amps or 230V 4 amps Space Requirements The dimensions for the non ventilated enclosures are shown in Appendix B Enclosure Dimensions Product Service 1 800 522 7658 5 CHAPTER 3 GETTING STARTED Now that the site for your non ventilated Purifier PCR or Purifier Tissue Culture Enclosures is properly prepared you are ready to unpack inspect and install your non ventilated enclosure Read this chapter to learn how to e Unpack and move the enclosure e Set up the enclosure with the proper supporting structure and work surface e Connect the electrical supply e Seal the enclosure to the work surface Depending upon which model you are installing you may need wrenches ratchets sockets a nut driver set a flat blade screwdriver a Phillips screwdriver and a carpenter level to complete the instructions in the chapter The enclosures weigh 100 Ibs 45 kg The shipping container allows for lifting with a mechanical lift truck or floor jack If you must lift the enclosure manually follow safe lifting guidelines Do not lift by the front lower support Product Service 1 800 522 7658 Chapter 3 Getting Started Unpacking the Enclosure The United States Carefully remove the shrink wrap or carton on the enclosure and I

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