USER`S MANUAL - Metertech Inc.
Contents
1. Figure 11 1 Lamp compartment CAUTION Switch off and disconnect the instrument from the mains supply and allow the lamp to cool down at least 15 minutes before proceeding WARNING Do not touch lamps with bare hands Use glove or cloth to prevent staining of the bulb WARNING Pay extreme attention not to touch parts other than those as instructed Replacement of tungsten halogen lamp Remove the cover of lamp compartment Unscrew the retaining screws screws a and b in Figure 11 1 of the holder of tungsten halogen lamp And pull the holder straightly upward Pull out the damaged lamp from the socket to remove Use lens paper to hold the new lamp for replacement Put the whole set back to the original position and screw it tightly Remount the cover of lamp compartment Replacement of deuterium lamp Remove the cover of lamp compartment Find the rapid connector with red black and blue wires and disconnect it Unscrew the retaining screws screws 1 and 2 in Figure 11 1 on the white aluminum seat for the 49 Metertech Inc deuterium lamp Pull the deuterium lamp straightly upward to remove Put anew deuterium lamp back to the holder and screw it tightly Reconnect the rapid connector of the deuterium lamp2. Metertech Inc Chapter 9 QUANTITATIVE This is the mode in which the concentration of an unknown sample under a specific wavelength can be determined by creating a calibration curve from standard samples Four methods for curve fitting can be selected straight line with or without passing through origin and 2 order polynomial with or without passing through origin In System Main Menu selecting item 5 will get into this mode Instrument and analysis parameters are set up in the QUANTITATIVE Parameter Setup screen Figure 9 1 Move the cursor to required parameter by using arrow keys and change setting of parameters Once parameters have been set press F5 Next Screen to get into the QUANTITATIVE Standard Table screen When standard samples are prepared ready press READ to proceed the measurement Once the standard table has been built up press function Manipulate to move on Users then can view the corresponding equation or measure new samples as needed Measuring results will be shown in the Measure Sample screen 12 00 00 OUANTITATIVE 500 0nm 0 000A Parameter Setup 1 Wavelenath nm 2 Standard number Conc S 1 0 0 S 2 0 0 S 3 0 0 3 Have Unit Yes No 4 Method L ord Oriain 5 Kevin ABS 6 Print everv cvcle Numgp rom U TO je Param Param Data Control Figure 9 1 Quantitative Parameter Setup screen Five functions are available in the first screen of this mode for users to select Load Param Save Param Pr
3. USERS MANUAL SP 8001 UV VIS Spectrophotometer Metertech Inc Version 1 08 IN Meterfech 63 2 Cheng Gong Road Sec 1 Nangang Taipei Taiwan R O C Tel 886 2 2783 2854 Fax 886 2 2783 1764 www metertech inc com info metertech com tw Metertech Inc provides this publication as is without warranty of any kind either express or implied including but not limited to the implied warranties of merchantability or fitness for a particular purpose All rights are reserved including those to reproduce this publication or parts thereof in any form without permission in writing from Metertech Inc Information in this publication is subject to change without notice and does not represent a commitment on the part of the vender Any errors or omissions which may have occurred in this publication despite the utmost care taken in its production will be corrected as soon as possible but not necessarily immediately upon detection Copyright Metertech Inc 1999 Metertech Inc 63 2 Cheng Gong Rd Sec 1 Nangang Taipei Taiwan R O C E Mail info metertech inc com Web Site www metertech inc com Tel 886 2 2783 2854 Fax 886 2 2783 1764 Metertech Inc Contents Chapter 1 INTRODUCTION Tune 4 81 1 Specification nun 4 81 2 Safety Notes aana 5 Chapter 2 INSTALLATION nun 6 2 1 Unpacking nun 6 2 2 Installation teen een eeeen 7 Chapter 3 GENERAL aenn 8 83 1 User Interface annan 8 83 2 General Functi
4. 2 Measure mode Have unit Yes No Unit Factor default 1 3 Cvcle number 4 Cvcle interval sec 5 Print everv cvcle NUMIDE O Param Param Data Control Figure 5 1 Photometric Parameter Setup screen Five functions are available in the first screen of this mode for users to select Load Param Save Param Print Data Sample Control and Next Screen 85 1 Photometric Parameter Setup Input the Number of For entering the number of wavelength 1 to 5 and the value s of wavelength to be used in the Photometric mode Measure Mode Selecting measurement results in ABS as for absorbance in T as for transmittance or in Conc as for concentration Cycle Number For entering the number of cycles 1 to 999 that a sample is required to be measured One sample being measured through all desired wavelengths is called as one cycle Cycle Interval sec For entering the time 0 to 9999 between the end of previous cycle 18 Metertech Inc and the beginning of next cycle Print Every Cycle Selecting Yes or No by pressing left right arrow keys to print at the end of every cycle 85 2 Photometric Function Structure Photometric Load Param Save Param Print Data Sample Control Next Screen rer Data Prev Screen For functions not described below please refer to General function description section 85 3 Basic Photometric Measurement The following is a description of the basic procedure for carrying out measurement in the SP
5. 3 and 2 99 for ABS and between 0 and 119 9 for T respectively High Value For entering the upper limit of measurement result between 0 29 and 3 00 for ABS and between 0 1 and 120 0 for T respectively The High Value must be at least 0 1 greater than the Low Value regardless the mode Scan Speed nm min For entering the Scan Speed in Spectrum mode between 100 and 5000 nm min Overlay Screen Selecting Yes or No to overlay the current measurement with previous measurements Note that only data for the current measurement can be manipulated 86 2 Spectrum Function Structure Load Data Spectrum Load Param Save Data Save Param es Access Data Print Data Prev Page Sample Control Smooth Data View Data Next Page Next Screen Manipulate Zoom Data Data Print Data Show Track Print Data Prev Screen Peak Valley View Data Prev Page Original Search Peak Next Page Clear Graph Search Valley Print Data Prev Screen Less Point Prev Screen More Point For functions not described below please refer to General function description section Access Data To load existing data from memory or to save data of current measurement into memory Smooth Data To smooth the data of current measurement in the screen Note that only the current measurement will go through this function and will be shown after completion of this function Zoom Data To zoom in the selected range of data in the current screen Key in proper values at blinking c
6. packing materials in a proper place in case that the instrument may need to be repacked or to be returned for service Metertech Inc 8 2 2 Installation Before the first installation please inspect carefully whether any damages or suspicious defects you may be aware of Do the followings before turn on the instrument Contact your local agent for help if encountering any difficulties during installation and operation A Always place the instrument on a stable and vibration free table Allow two inches behind the rear panel of the instrument for air circulation and power cord and protect the instrument from being located indirect sunlight in drought from window air conditioner or heater near copy machine near refrigerator or oven near ultrasonic cleaner near X ray equipment near centrifuge B Check and set the voltage The SP 8001 can be performed with selective power supply at 100 120 220 or 240Vac 50 60Hz from a properly wired and grounded single phase power outlet Check the voltage shown in the frame above power inlet If it is not the desired use flat screw driver to open the protective plastic cover Push inside the voltage wheel and turn it into the desired position accordingly Close the protective plastic cover C The room temperature of operating site should be between 15 30 C and humidity less than 80 D Install all desired accessories and connect necessary wires and tubes properly
7. 8001 Photometric measurement mode For example five samples are ready to proceed photometric measurement in 4 wavelengths 400 450 500 and 550 Step 1 In System Main Menu select 1 then press ENTER to get into the Parameter Setup screen Step 2 In Parameter Setup screen Figure 5 2 key in the number of and the corresponding values of Each entry is followed by an ENTER key Then go through other items 2 to 5 with proper entries as instructed If any setting needs correction use up down arrow keys to move the cursor 12 00 00 PHOTOMETRIC 500 0nm Parameter Setup 1 1 nput the Number of 40 2 450 3 500 4 550 2 Measure mode Have unit Yes No Unit Factor default 1 3 Cvcle number 4 Cvcle interval sec 5 Print everv cvcle to Number from 1 ae Param Param Data Control Figure 5 2 Setting of parameters 19 Metertech Inc to the proper position When all is done press F5 Next Screen to next step Step 3 Get a sample ready and press READ The reading of that sample will be displayed on the screen Repeat this step for other four samples Readings of measurements will be displayed in the screen Figure 5 3 Step 4 Data obtained in every measurement can be printed by pressing F3 Print Data To get back to Parameter Setup screen press ESC or F5 Prev Screen 12 00 00 PHOTOMETRIC 550 0nm D D gt L Ec AD wnen Data Screen Figure 5 3 Photometric readings of samples A very simple way to p
8. 90 t N o 1100 Param Param Data Control Figure 7 2 Setting of parameters 32 Metertech Inc Step 4 After the measurement functions can be selected in the same way as in Spectrum mode 72 00 08 TIMESCAN 500 0nm 101 0 T 100 99 0 O sec 150 300 Pre SCAD wnen reac fo fom em Ein Data e Graph Screen Figure 7 3 Measurement in TimeScan screen Metertech Inc Chapter 8 KINETIC In this mode the time dependent changes in absorbance ABS at a fixed wavelength are measured and recorded A typical application in this mode is the measurement of an enzyme activity The time changes in absorbance will be displayed on screen and the absorbance can be found at each sampling interval set according to Lag Time plus with Rate Time The segment after measurement has started which is not subject to activity computation is set as Lag Time The amount of change in absorbance is calculated as Rate Time that is evenly divided The rate of change in absorbance is calculated with the least square method and can be recalculated for different segments by resetting the Lag Time and Rate Time for the same graph In System Main Menu selecting item 4 will get into this mode Instrument and analysis parameters are set up in the KINETIC Parameter Setup screen Figure 8 1 Move the cursor to required parameter by using arrow keys and change values of parameters Once parameters have been set press function View Graph to get into the K
9. ATIVE 500 0nm Measure Sample Conc G L 1 E 5 2100 2 F 11 370 3 RED NT read Data Control Screen Figure 9 5 Quantitative Measure Sample screen Chapter 10 ACCESSORIES 12 00 00 OUANTITATIVE 500 0nm Linear Fittina Conc K1 ABS KO K1 10 00000 KO 0 00000 r 1 00000 BE pe Eea Data Screen Figure 9 6 quantitative Linear Fitting screen Metertech Inc designed several optional accessories to meet users specific purposes while the Single cell holder is a standard accessory When an optional accessory is to be used users should turn off the instrument first and then do the hardware installation as well as the cable connection The SP 8001 will automatically detect attached accessory When the instrument is turned on the detected accessories will be shown during initialization After initialization pressing function Sample Control will get into screens to set up the parameters of accessories Whenever function Sample Control shows up in various screen it can be pressed to run the accessory accordingly A function named Prev Screen will close up the operation of accessories and back to 43 Metertech Inc the previous screen The optional accessory can be disabled in the System Setup by selecting item 5 Setup Hardware however it has to be detected during the initialization process Hot plug is not recommended and it may cause damage 810 1 Multi Cell Holder The Multi Cell Holder is an optional acc
10. E Plug the power cord into the instrument inlet and to an appropriate wall outlet F Now the system is ready for performance Turn power on and refer details of operation to specific sections Metertech Inc Chapter 3 GENERAL The SP 8001 is designed to be a self contained instrument and can work as a standalone machine or can work with other peripherals together The SP 8001 has one membrane type keyboard and a 320X240 LCD display as the user interface User may key in data through the keyboard and proper messages will show on the display A lamp compartment facilitates a tungsten halogen lamp and a deuterium lamp that users are allowed to replace those lamps whenever needed READ SAFETY NOTES in section 1 2 carefully and instructions in section 11 2 before changing lamps A sample compartment is the area for users manipulating their samples Also it allows users to install proper accessories for specific operations 3 1 User I nterface The SP 8001 keyboard with LCD display module is shown in Figure 3 1 Operations of those keys are designed to be user friendly In most of cases users press keys according to instructions shown in the corresponding screens Otherwise refer to specific sections of different operation modes for more details Figure 3 1 Keyboard with LCD display module For operations with optional accessories refer to proper sections in Chapter 10 General explanation of keys is given below Function k
11. INETIC measuring graph screen When samples are prepared ready press READ to proceed the measurement Measuring results will be shown in the screen 12 00 00 KINETIC 500 0nm 0 000A Parameter Setup 1 Wavelenath nm 2 Samplina Number 3 Laa Time sec 4 Rate Time sec 5 Delav Time sec 6 Have unit Yes No 7 Factor default 1 8 Low Value ABS 9 Hiah Value ABS 10 Measure Temperature 11 Overlav Screen 12 Print everv cvcle to 90 Number from 2 N Param Param Data Control Figure 8 1 Kinetic Parameter Setup screen Five functions are available in the first screen of this mode for users to select Load Param Save Param Print Data Sample Control and Next Screen 34 Metertech Inc 88 1 Kinetic Parameter Setup Wavelength nm Sampling number Lag Time sec Rate Time sec Delay Time sec Have Unit Yes No Factor default 1 Low Value ABS High Value ABS Measure Temperature Overlay Screen Print Every Cycle For entering the value of wavelength to be used in the Kinetic mode The value should range from 190 to 1100 For entering the sampling number of a measurement in the Kinetic mode This number of data is used for the activity calculations The value should range from 2 to 20 For setting the time in second from the start of measurement during which data will not be acquired for activity calculations The value should range from 2 to 9999 For setting the time in second after
12. JE Device Manager Fie Action View Help e E Efa f A er E E USER E y Computer a Disk drives H Display adapters Y DYD CO ROM drives H Floppy disk controllers Cl Floppy disk drives IDE ATAJATAPI controllers H Keyboards pT Mice and other pointing devices 6 9 Monitors Eg Network adapters I Ports COM amp LPT S wen a Communications Port COM1 Z a F Communications Pork COZ oo ECP Printer Port LPT1 L he USE Serial Port COM P Processors Y Sound video and game controllers System devices E Universal Serial Bus controllers IE Es Figure C 2 USB Serial Port COM 3 Properties Port Settings Bits per second al Data bits Parity Stop bits Flow control None Advanced Restore Default Figure C 3 57 Metertech Inc Advanced Settings for COM3 Figure C 4 58
13. P 8001 UV VIS Spectrophotometer is an instrument designed and manufactured by Metertech Inc It can meet the wide ranging needs of prospective users The main unit features fundamental photometric functions that are ready expandable using various application software packages and attachments for enhanced convenience and productivity It is designed to be user friendly and require as little engineering knowledge as possible to adopt in users application In order to obtain maximum performance and trouble free operation with the SP 8001 It is recommended that you carefully read this manual before using the SP 8001 and thereafter keep it close at hand for future reference 81 1 Specifications Wavelength Bandwidth pass Wavelength accuracy Monochromator Wavelength repeatability Photometric range Photometric accuracy Stability Baseline flatness Stray light Light source Source lamp change Detector Wavelength scanning speed Path length Display Interface Power fuse Dimension Weight Ambient temp humidity 190 1100nm 2nm inm split beam 0 2nm 0 300 3 000Abs 0 005Abs at 1 000Abs by NIST SRM930e drifting lt 0 0003Abs hr at 500nm after 1 hour warm up 0 002Abs at 210 1000nm lt 0 05 at 340nm amp 220nm by NIST SRM2031 deuterium lamp UV range amp halogen lamp Vis range automatic Silicon photodiodes 100 5000nm min user programmable variable at 10 100 mm backlight LCD a
14. Remount the cover of lamp compartment WARNING For replacing deuterium lamp or halogen lamp be sure to contact your local agent and use vendor s designated light sources Light source specifications Halogen lamp 12V 20W and lifetime 2000 hrs Deuterium lamp ignition voltage 500V operating voltage 75 95V operating current 300mA and lifetime 500 hrs 50 Metertech Inc Appendix A THERMAL CONTROLLER OPERATION 8 A 1 Installation of thermal controller on SP8001 machine Power off SP8001 machine The thermal controller can be applied to SP8001 in two approaches It can be an independent unit for rectangular cuvette or accompanied with sipper for flow through cell For a standard alone thermal controller a 24 pin to 9 pin cable adapter is required For operating with sipper please refer to the operation instruction of sipper Power off SP8001 machine Plug the cable adapter into receptacle at the bottom right wall of SP8001 cell compartment Remove single cell holder by loosing three M3 screws locate the cell holder of thermal controller at the same position as single cell holder did Then tighten it with three M3 screws Connect the 9 pin connector of thermal controller with that of cable adaptor if sipper is required connect 9 pin connector of thermal controller to that of sipper A 2 Operation of thermal controller Power on SP8001 machine For a proper thermal controller installation a message D
15. ad B Line is available 500 0nm 0 000A Svstem Setup Menu 1 Setup Clock 2 Setup Lamp 3 Setup Printer 4 Setup RS 232 5 Setup Hardware 6 Measure Bandwidth 7 Measure Base line 8 Search Zero Position Select Number 1 Figure 4 1 System Setup Menu screen Load B Line Will reload the results of Base line measurement 4 1 System Setup Menu Setup Clock To set values Figure 4 2 for Month Date Year Hour Minute and 12 00 00 500 0nm 0 000A Clock Parameter Setup 1 Month 2 Date 3 Year 4 Hour 5 Minute 6 Second 1 to 1 Number from Ques Data Screen Figure 4 2 Clock Parameter Setup screen 14 Metertech Inc Second as necessary Setup Lamp Use function keys to set up parameters for visible and UV lamps Figure 4 3 Switch UV is for switching ON OFF of UV lamp Reset Vis Hr is for resetting the usage time of the tungsten lamp Reset UV Hr is for resetting the usage time of the UV lamp Energy Vis is for 12 00 00 500 0nm 0 000A Lamp Parameter Setup 1 Visible lamp status ON 2 Visible lamp used hours 20 3 Visible lamp enerav 100 5 UV lamp used hours 20 6 UV lamp enerav 100 4 UV lamp status ON Switch Reset Reset Enerav Enerav UV Vis Hr UV Hr Vis UV Figure 4 3 Lamp Parameter Setup screen measuring the power of the tungsten lamp Energy UV is for measuring the power of the UV lamp Setup Printer Four printers SII DPU 41X Epson 9 24 Pin Epson Stylus and HP Deskjet Laserjet
16. alleys in the current graph The corresponding data can be viewed under View Data function Original To redraw the graph from data obtained in the current measurement 6 3 Basic Spectrum Measurement The following is a description of the basic procedure for carrying out measurement in the SP 8001 Spectrum measurement mode For example a spectrum of sample is to be measured from 400nm to 800nm and the results should be bounded between 0 and 1 5Abs Scanning speed is set at 1000nm min Step 1 In System Main Menu select 2 then press ENTER to get into the Parameter Setup screen Step 2 In Parameter Setup key in desired values or select proper choice in those 7 items step by step followed by an ENTER Figure 6 2 If any setting needs correction use up down arrow keys to move the cursor to the proper position When all is done press F5 Next Screen to get into the Spectrum screen 25 Metertech Inc 12 00 00 SPECTRUM 500 0nm Parameter Setup 1 Start wavelenath nm 2 Stop wavelenath nm 3 Measure mode 4 Low value ABS 5 Hiah value ABS 6 Scan speed nm min 7 Overlav screen Numd rom D TO LOIG Param Param Data Control Figure 6 2 Setting of parameters Step 3 Make the sample ready and press READ The measurement result will be displayed in the Spectrum screen Figure 6 3 in real time mode The upper right corner of the screen will show the current wavelength and the current reading Step 4 When parameter Overla
17. aram and you may see what has just been done Print Data To print data or graph from the current screen Sample Control To be functioning only when certain accessory being used 10 Metertech Inc 500 0nm 0 000A 6 4 5 7 8 9 Figure 3 4 Filename input screen Next Screen Accept the current settings and switch into the measuring display screen Once get the sample s ready press READ for proceeding the measurement according to the setup Sometimes it is used for tracing back to the previous level of functions or for showing the previous graph on the screen Prev Screen Leave the current display screen and switch back to a previous display screen Manipulate For further manipulation of current data either from new measurement or from existing data files Clear Graph To clean up the current screen This will result in loss of data in previous measurement Only those data for the most current measurement can be rebuilt with function Original under function Manipulate Load Data To load data with determined parameter setup from the associated Data File List Save Data To save the data of current measurement or to save the data of chosen point in function Show Track This will also save the settings of parameters in that measurement Message No data present will show if there is no data in the screen l1 Metertech Inc 8 3 3 Initialization Turn on the power of the SP 8001 a start up screen Figure 3 5
18. ata 0 0000 A Pre amp kevin wavelenath Data Data A C T Control Digits Figure 5 6 Five digit reading 21 Metertech Inc Step 6 After data saved pressing F1 View Data can get into the Data File List screen Figure 5 8 It will show the first 15 sets of data being saved In this screen functions F1 Prev Page is for viewing Pe LAMBDA 500 0nm 500 0 nm Data 0 000 A Figure 5 7 Saving data of measurement previous page and F2 Next Page is for viewing next page Pressing F3 Print Data will print out all sets of data 60 sets the maximum The saved data will be erased once users exit from this mode LAMBDA 600 0nm Data File List Data0l 500 0nm 0 000A Data02 500 0nm 100 0 T Data03 400 0nm 1 200A Data04 500 0nm 72 0 T Data05 600 0nm 0 000A Page Page Data Screen Figure 5 8 Data File List screen 22 Metertech Inc Chapter 6 SPECTRUM This is the mode in which spectral measurement is performed Measured spectra can be saved to memory and be subjected to various data manipulation described in details below The measured spectra can be zoomed by changing the interested range Interested point of the spectra can be tracked manually and the corresponding data will be shown in the screen Peaks or valleys of measured spectra can be automatically picked up and corresponding data can be shown in the screen The number of interested peaks or valleys can be changed in the same spectra Multiple spectra can be vi
19. can be selected through arrow keys as instructed Figure 4 4 15 Metertech Inc 12 00 00 500 0nm 0 000A Serial Port Parameter Setup 1 Baud rate 2 Bit lenath 3 Paritv check 4 Stop bit 5 Remote status Data Screen Figure 4 5 Serial Port Parameter Setup screen Setup RS 232 Parameters for RS 232 can be set up through instructions shown in the screen Figure 4 5 Setup Hardware In this option three adjustable items are shown Figure 4 6 Use arrow keys to move cursor to proper position and proceed as 12 00 00 500 0nm 0 000A Accessorv Parameter Setup 1 UV VIS chanae wavelenath eee 2 Switch to Sinale Cell 3 Scan base line at POWER ON No 12 00 00 500 0nm 0 000A Printer Parameter Setup 1 Printer Tvpe EPSON STYLUS Figure 4 4 Printer Parameter Setup screen instructed at the bottom of this screen UV VIS Change Wavelength 16 Metertech Inc 12 00 00 BANDWIDTH 676 0nm 100 0 1 50 0 0 0 636 656 676 nm 1 655 59nm 657 44nm Bandwidth 1 85nm max 656 12nm Mez ament compl i Data Screen Figure 4 7 Bandwidth measurement screen allows users to set switching point for UV VIS lamps while 363nm is set as default Switch to Single Cell allows users to switch accessories between Single Cell and accessories e g Multi Cell or Sippe Scan Base Line at Power On allows users to determine whether to execute base line measurement or not right after turning on the power Measu
20. creen Manipulate Sample Measure Print Data sampi Control Prev Screen View Curve u Data Prev Screen Prev Screen Prev Screen For functions not described below please refer to General function description section Access Data To load existing data from memory or to save data of current status into memory Here data refers to those factors of fitted equation with parameter setup in the Quantitative mode Use ESC key to get back to the function level View Equati To view the fitted equation and associated factors under determined fitting method View Curve To view the fitted equation in graph representation with tiny squares representing data for standard samples Keyin Factor For entering factor values for the fitted equation Sample Measure To get into the QUANTITATIVE Measure Sample screen in order to 40 Metertech Inc read samples whenever ready 89 3 Basic Quantitative Measurement The following is a description of the basic procedure for carrying out measurement in the SP 8001 Quantitative measurement mode In this mode users use standard samples to determine unknown samples For example there are three standard samples with known concentration and absorbance If the absorbance values are unknown users can also use the SP 8001 to find out the corresponding absorbance values Using those data the relationship between concentration and absorbance can be build up through curve fitting technique Once the equation being constru
21. cted users can use it to determine concentrations of unknown samples by reading the absorbance from the instrument Step 1 In System Main Menu select 5 then press ENTER to get into the Parameter Setup screen Step 2 In Parameter Setup screen Figure 9 2 key in required values in the corresponding parameters Each entry is followed by an ENTER If any setting needs correction use Up Down arrow keys to move the cursor to the proper position Here set wavelength at 500 nm In item 2 input 3 followed by an ENTER key Then in the corresponding fields key in concentration values followed by ENTER keys Select U L as the unit in item 3 Select 1 ord Origin in item 4 Method In item 5 set Yes for later keying in absorbance value manually After all parameters set up press F5 Next Screen to next step Step 3 In the Quantitative Standard Table screen Figure 9 3 press READ to start to key in absorbance value for corresponding concentration After finishing the key in operation press ESC to exit Now 12 00 00 OUANTITATIVE 500 0nm 0 000A Parameter Setup 1 Wavelenath nm 2 Standard number Conc S 2 2 0 3 Have Unit Yes No 4 Method T ord Oriain 5 Kevin ABS 6 Print everv cvcle Number from 190 to 1100 Param Param Data Control Figure 9 2 Setting of parameters press F2 Manipulate to go to next step After F2 Manipulate being pressed the SP 8001 will proceed calculation for the input data and an equation
22. describing the relation of input data will be build up 41 Metertech Inc Step 4 Here different function keys will show in the bottom of this screen figure 9 4 Press F2 12 00 00 OUANTITATIVE 500 0nm Standard Table Number from 0 3 to 3 0 Fe 9 Data oulate Screen Figure 9 3 Standard Table screen Sample Measure to start measurement for unknown samples and it is described in step 5 Pressing F1 View Equati will allow users to view details of the fitted curve and it is described in step 6 Step 5 In the Quantitative Measure Sample screen Figure 9 5 get the unknown sample ready and press READ then the SP 8001 will measure the absorbance of the sample and calculate the 12 00 00 OUANTITATIVE 500 0nm Standard Table Sample No Conc G L 1 1 0000 2 2 0000 3 3 0000 Press hot kev to process Uy ae ne Ee Equati Measure Curve Screen Figure 9 4 More functions in Standard Table corresponding concentration by using the equation obtained before Results will be shown in the screen and can also be printed by pressing F3 Print Data Step 6 In the Quantitative Linear Fitting screen Figure 9 6 details of the fitted curve will be shown The equation describes the relation between concentration and absorbance value Ki and KO are the coefficients in the equation and depend on the selected method of curve fitting r shows the correlation coefficient for the fitted equation 42 Metertech Inc 12 00 00 OUANTIT
23. djustable contrast serial amp parallel AC input 100 120 220 240V 50 60Hz Fuse T2A for 220 240V T4A for 100 120V 506 W X430 D X220 H mm approx 18kgs room temperature 15 35 C humidity lt 80 Specifications subject to change without notice Metertech Inc 81 2 Safety Notes Although this SP 8001 UV VIS Spectrophotometer is user friendly the instrument and its accessories described in this manual are designed to be used by properly trained persons only READ THIS SECTION CAREFULLY BEFORE INSTALLING AND USING THE INSTRUMENT AND ITS ACCESSORIES v Adjustment maintenance and repair of equipment must be carried out only by qualified persons who are aware of possible hazards involved Wherever indicated in this manual certain maintenance may be carried out by users who must be fully understand and apply the following safety precautions v _ For the correct and safe use of this instrument and its accessories it is essential that both operating and servicing persons follow generally accepted safety procedures in addition to the safety precautions specified in this manual v Some of the chemicals used in spectrophotometer are corrosive and or flammable and samples may be radioactive toxic or potentially infective Care should be taken to follow the normal laboratory procedures for handling chemicals v Only the persons who have been trained to avoid the risk of electric shock are qualified to remove the instrument covers and access
24. down the bottom surface of cell holder with a cross type screw driver If you are using flow cell for experiment and want get the best light transmission through cell aperture Please fill pure water into cell and make sure no air bubble inside the aperture At the Lambda mode of SP8001 key in 500nm remove cell from cell holder and AUTOZERO to get 100 T Put in flow cell with water loose three screws of thermal controller and move it forward backward with respect to front panel until getting reading greater than 50 T 32 Metertech Inc Appendix B SIPPER OPERATION 8B 1 Installation of sipper on SP8001 machine Power off SP8001 machine Remove the front panel of cell compartment of SP8001 by loosing two M3 screws behind it A sipper assembly as shown on fig 1 includes one pumping drive one pinch valve one sample input and another air input two connectors small one for connecting to electronically thermal controlled cell assembly large one for connecting to SP8001 machine two flexible tubes and front panel The latter one contains sample solution inlet and outlet thermal controlled water circulation inlet and outlet and one button Locate the sipper assembly above the cell compartment plug its large connector into receptacle on the bottom right wall of cell compartment set connector cable under sipper assembly Move down the sipper until touching bottom surface then tighten the sipper on the bottom surface with o
25. erform photometric measurement if users only need to get readings of samples in a specific wavelength Step 1 In System Main Menu press in the keyboard to get into the Lambda screen Figure 5 4 Step 2 Get the sample ready for measurement In the Lambda screen key in the value for wavelength 12 00 00 LAMBDA 500 0nm 500 0 nm Data 0 000 A Pre amp kevin wavelenath View Save Mode Sample Toggle Data Data A C T Control Digits Figure 5 4 Lambda screen 20 Metertech Inc and press ENTER The reading of the measurement will show up in the screen Step 3 After the measurement press F3 Mode A C T can switch reading into different formats A as for Abs C as for concentration and T as for transmittance Value of factor will be asked for those choosing Mode in C Figure 5 5 Step 4 When the reading is in ABS format user may press F5 Toggle Digits to show five digits for more precise reading Figure 5 6 This function can only work under the ABS format Step 5 After the measurement pressing F2 Save Data can save the current reading The saved data 12 00 00 LAMBDA 500 0nm 500 0 nm Data 100 0 T Factor 1 000 From 1 to 9999 Pre avin wavele e amp k lenath View Sav Mode Sample Toggle Data Data A C T Control Digits Figure 5 5 Reading in T format will be displayed at the bottom of the screen Figure 5 7 Up to 60 sets of data can be saved 12 00 00 LAMBDA 500 0nm 500 0 nm D
26. ers want to trace readings of measurement result function Show Track is designed for this purpose Pressing F3 Show Track will make a dotted vertical line showing up in the graph Figure 6 6 Use left right arrow keys to move the dotted line one pixel in the corresponding direction Use up down arrow keys to move dotted line ten pixels in right and left directions respectively 21 Metertech Inc 12 00 00 SPECTRUM 533 2nm Press lt gt kev to move Data Data Data Figure 6 6 Graph under Show Track function During the tracing process corresponding values for traced point will be displayed at the up right corner in the screen Step 8 Under Show Track mode data of the traced point can be saved by pressing F2 Save Data And a number will be assigned automatically to that traced point The number will be displayed at the lower right corner in the screen Figure 6 7 Total 60 sets of data can be saved In this mode pressing F3 Print Data will print out this screen with the dotted line Pressing Fi View Data will show the Data File List screen that displays all saved data in the Show Track mode 12 00 00 SPECTRUM 562 4nm 600 Data No 05 Pre lt gt KE move Data Data Data Figure 6 7 Saving data in Show Track mode Step 9 In the Spectrum screen pressing function Manipulate and then function Peak Valley will get into the mode that peaks and valleys of the graph can be picked up automatically In this searching mode
27. essory that enables up to 6 cells to be presented for measurement sequentially Put the Multi Cell Holder into the sample compartment and fix it with 4 screws as shown in Figure 10 1 Plug the 25 pin male connector into the corresponding female connector in the sample compartment Turn on the instrument and it will be automatically detected Slider Cell holder L S 25 pin male connector _ IT Figure 10 1 Multi Cell Holder When the Multi Cell Holder is installed pressing Read will perform measurements of all cells being set according to Drive Cell Number Also at the right bottom corner of every screen a note shows the current Cell number that is in the measurement position A Multi Cell Parameter Setup screen Figure 10 2 will show up when function Sample Control being pressed Detailed settings and respective functions will be described below 44 Metertech Inc 12 00 00 500 0nm 0 000A Multi Cell Parameter Setup 1 Driver cell Numbert 1 6 2 2 Reacdent blank corr cell 1 Yes all from 1 toa all l She Senan San Ein Home Standby Blank Scree Figure 10 2 Multi Cell Parameter Setup screen Driver Cell Number 1 6 For entering the number of cells to be measured The value should range from 1 to 6 Reagent Blank Corr Cell 1 Selecting Yes to confirm that Cell 1 is used as Blank and No for using base line as blank Three functions are available in this screen Cell Home Cell Standb
28. etect Accessory Single cell TC or Sipper TC under the company logo window of LCD display will be shown If it displays Detect Accessory Single Cell please power off machine and check thermal controller installation until TC message is appeared The default temperature setting is 25 C when the machine is started If you can not get the correct reading at the bottom right corner of LCD after 5 minutes from the machine was powered please turn off machine and inspect the cause of problem Select 6 under System Main Menu of LCD display and go to the System setup menu Then pick 5 and go to Accessory parameter setup Using up down arrow key to select 4 setup temperature here you can choose one temp setting from 37 C 30 C 25 C and none no heating or cooling action by left and right arrow key Finally press ENTER key the temp controller starts to climb up or fall down to your last temp setting The settling time from one temp to another is less than 5 minutes and temp will be stabilized within O 1C Return to system main menu pick 4 kinetic mode Pick 10 measure temp of the parameter setup you can also select the temp setting for your experiment 51 Metertech Inc 8 A 3 Important notes The thermal controller is designed to adapt for two types of flow cell aperture height with 8 5 mm and 15 mm The default height is 8 5 mm If 15 mm height is needed please remove an aluminum block right
29. ewed in the same graph for comparison purpose In System Main Menu selecting item 2 will get into this mode Instrument and analysis parameters are set up in the SPECTRUM Parameter Setup screen Figure 6 1 Move the cursor to required parameter by using arrow keys and change values of parameters as needed Once parameters have been set press F5 Next Screen to get into the SPECTRUM measuring graph screen When samples are prepared ready press READ to proceed the measurement Measuring results will be shown in the screen Five functions are available in the first screen of this mode for users to select Load Param Save Param 12 00 00 SPECTRUM 500 0nm Parameter Setup 1 Start wavelenath nm 2 Ston wavelenath nm 3 Measure mode 4 Low value ABS 5 Hiah value ABS 6 Scan speed nm min 7 Overlav screen NUMIDE O OG Param Param Data Control Figure 6 1 Spectrum Parameter Setup screen Print Data Sample Control and View Graph 6 1 Spectrum Parameter Setup Start Wavelength nm For entering the Start Wavelength in Spectrum mode between 190nm and 1098nm Stop Wavelength nm For entering the Stop Wavelength in Spectrum mode between 192nm and 1100nm The Stop Wavelength must be at least 2nm greater than the Start Wavelength 23 Metertech Inc Measure Mode Selecting measurement results in ABS as for absorbance or in T as for transmittance Low Value For entering the lower limit of measurement result between 0
30. eys Selecting and performing supporting functions shown in the screens Numerical keys For entering numbers minus and decimal point For entering desired wavelength CLEAR To clean up unwanted input digits in the entry field ESC To quit the current screen and go back to the previous screen or to quit from the current process or to go back to upper level of function key screen QUICK RUN To pop up a Parameter File List Figure 3 2 for users to select operation settings 8 Metertech Inc stored in memory In this file list users can view all parameter settings in different operation modes that is distinguished by the first 2 letters in the first column For example PH represents for Photometric mode Filenames are shown in the second column while the corresponding time in the third column READ To start measuring process according to the current settings AUTO ZERO To perform blank correction when needed It sets the photometric value to OABS 12 00 00 OUICK RUN 500 0nm 0 000A Parameter file list HOLMI UM 07 12 99 09 30 12 LI GHTO1 06 24 99 13 22 43 LIGHTO2 06 25 99 15 32 33 03 21 99 16 37 21 Figure 3 2 Quick Run Parameter File List screen 100 T after AUTO ZERO being pressed ENTER To confirm selections or entries of fields or parameters Arrow keys To move the cursor between entry fields or parameters Left right arrow keys are also used for switching Yes No as instructed CELL To move sa
31. hite roller while wheel is rotating turn the stop fig 1 45 90 degrees cw cww until the tube returning to center position The peristaltic tube at abnormal condition may not be tightened enough across pumping wheel such that the sipping force is too small to displace proper amount of solution into the cell Please check the tube fitness into the stop or cut the tube shorter 1mm off at one time to generate more elastic force If flow through cell is used for measurement cell alignment for max light to pass through aperture is required Fill the flow cell with water either by sipping or other method then go to Lambda mode and set wavelength 500 nm remove flow cell from cell holder enter AUTOZERO and obtain 100 T Put cell back to cell holder and cover the cell compartment a good reading would be larger than 50 T for aperture diameter 2 mm If reading is smaller than 40 T please loose three M3 screws around the cell holder and move it forward backward with respect to front panel there is only 2 mm max moveable displacement until finding the max reading Finally tighten these screws 54 Metertech Inc flow cell amp tubes peristaltic tube pinch valve button stop 9 pin small connector screw hple sample inlet 24 pin large connector sample outlet pumping wheel thermal water inlet amp outlet Figure 1 Sipper Assembly with flow cell connected 55 Metertech Inc Appendix C SETTING OF USB SERIAL PORT If y
32. hown in the screen 12 00 00 TIMESCAN 500 0nm Parameter Setup 1 Wavelenath nm 2 Scan time sec 3 Delav time sec 4 Measure mode 5 Low value ABS 6 Hiah value ABS 7 Overlav screen MUITIDE Or vie Param Param Data Control Figure 7 1 TimeScan Parameter Setup screen Five functions are available in the first screen of this mode for users to select Load Param Save Param Print Data Sample Control and Next Screen 8 7 1 TimeScan Parameter Setup Wavelength nm For entering the value of wavelength to be used in the TimeScan mode The value should range from 190 to 1100 Scan Time sec For setting the time in second over which the sample will be measured The value should range from 5 to 3000 Delay Time sec For setting the time in second between pressing READ and the start of the measurement The value should range from 0 to 50 Measure Mode Selecting measurement results in ABS as for absorbance or in T as for transmittance 30 Metertech Inc Low Value For entering the lower limit of measurement result between 0 3 and 2 99 for ABS and between 0 and 119 9 for T respectively High Value For entering the upper limit of measurement result between 0 29 and 3 00 for ABS and between 0 1 and 120 0 for T respectively The High Value must be at least 0 1 greater than the Low Value regardless the mode Overlay Screen Selecting Yes or No to overlay the current measurement with previous measurements Note that onl
33. int Data Sample Control and Next Screen 89 1 Quantitative Parameter Setup Wavelength nm For entering the value of wavelength to be used in the Quantitative mode The value should range from 190 to 1100 Standard Number Conc For entering the number of standard samples used in the process The value should range from 1 to 10 Concentration for each standard sample can also be entered in the corresponding fields 39 Metertech Inc Have Unit Yes No Selecting the unit option for measurement results When Yes is selected pick the right unit out of the thirteen build in units in table Method Four methods of data fitting are available 1 ord Origin for straight line through origin 1 ord No Org for straight line not passing through origin 2 ord Origin for 2 ordered polynomial through origin and 2 ord No Org for 2 ordered polynomial without passing through origin Keyin ABS Selecting Yes for users to manually key in values of ABS Selecting No for instrument to read ABS values of standard samples automatically When first time get into the QUANTITATIVE Standard Table screen press READ to initiate this procedure Print Every Cycle Selecting Yes or No to print at the end of every measurement 9 2 Quantitative Function Structure Quantitative Load Param Load Data Save Param Print Data Save Data l Keyin Factor Print Data Print Data Access Data Sample Control View Equati Prev Screen Next S
34. mple holder one position up as needed CELL v To move sample holder one position down as needed Metertech Inc 83 2 General Function Description Functions being used in the SP 8001 are listed in the corresponding modes and are shown in structured maps For those functions being widely used in most of the modes will be described below Load Param For loading existing parameter settings stored in memory When this function is pressed a parameter file list screen Figure 3 3 shows up and users can choose from the list by using arrow keys and followed by an ENTER key Only the mode relevant parameter setting files will appear when function Load Param is pressed Save Param For saving a current settings into memory Filename is entered 12 00 00 PHOTOMETRIC 500 0nm 0 000A Parameter file list Figure 3 3 Parameter file list in photometric mode through a 4 by 9 array type table Figure 3 4 By using up down arrow keys for choosing rows and then numerical keys for choosing column the desired alphabet or number will be picked up 10 alpha numerical keys are the maximum for a filename Using left arrow key to delete the input An ENTER key will close the input procedure and the Parameter File List screen will show up Using up down arrow keys to choose a place for the filename and followed by an ENTER key will complete the Save Param procedure A time tag will be automatically attached to the saved filename Press function Load P
35. ne M3 screw located at opposite side of sipper s front panel and one M4 screw beside the pumping wheel Connect two flexible tubes of sipper to those of flow through cell make sure the length of cell s both tubes are cut properly with length 55 mm B 2 Operation of sipper Power on SP8001 machine For a proper sipper installation a message Detect Accessory Sipper under the company logo window of LCD display will be shown If it displays Detect Accessory Single Cell please power off machine and check sipper installation until Sipper message is appeared Press F4 Sample Control under System Main Menu of LCD display and you are going to the sipper parameter setup mode For a first time using sipper there is a set of default parameters shown on the LCD these numbers are suitable for a 10 mm long flow through cell with aperture diameter 2mm and aperture height 8 5 mm It gives minimum sipped sample volume around 700 ul with reasonable carryover depends on the sample viscosity Key Fi wash cell is for continuously cleaning the cell and tube for 20 seconds F2 sipper test is to test the sipper operation with latest parameters setting F3 for printing out the whole parameter setup F4 for retrieving default parameter saved in ROM F5 amp Esc for going back to last screen The keyed in parameters will be saved in memory 53 Metertech Inc There are six parameters to be set for the sipper to be prope
36. om this function View Data To review data being saved under function Show Track or being found under function Peak Valley Prev Page To review data in the previous page Only 15 sets of data can be displayed in one screen Next Page To review data in the next page Only 15 sets of data can be displayed in one screen Peak Valley For users to find peaks or valleys in the current graph Original To redraw the graph from data obtained in the current measurement 8 7 3 Basic TimeScan Measurement The following is a description of the basic procedure for carrying out measurement in the SP 8001 TimeScan measurement mode For example users want to know the measurement change of a sample in a time span of 300 seconds at wavelength 500nm Step 1 In System Main Menu select 3 then press ENTER to get into the Parameter Setup screen Step 2 In Parameter Setup screen Figure 7 2 key in required values in the corresponding parameters Each entry is followed by an ENTER key If any setting needs correction use up down arrow keys to move the cursor to the proper position When all is done press F5 Next Screen to next step Step 3 Make the sample ready and press READ The measurement result will be displayed in the TimeScan screen Figure 7 3 in real time mode 12 00 00 TIMESCAN 500 0nm Parameter Setup 1 Wavelenath nm 2 Scan time sec 3 Delav time sec 4 Measure mode 5 Low value ABS 6 Hiah value ABS 7 Overlav screen mber from 1
37. on Description ennenen ennn e 10 3 3 Initialization eee ee neeeee 12 Chapter 4 SYSTEMSETUP On 14 84 1 System Setup Menu nun 14 Chapter 5 PHOTOMETRIC nun 18 85 1 Photometric Parameter Setup OO tne nenennnnncncene 18 85 2 Photometric Function Structure tenn ne nnnnnnncneens 19 85 3 Basic Photometric Measurement essen ennenen e 19 Chapter 6 SPECTRUM nun 23 86 1 Spectrum Parameter Setup oO mn 23 86 2 Spectrum Function Structure eenn 24 86 3 Basic Spectrum Measurement enesenn nnn nnn nee 25 Chapter 7 TTMESCAN nun 30 87 1 TimeScan Parameter Setup ennenen 30 87 2 TimeScan Function Structure eenn 31 87 3 Basic TimeScan Measurement enesenn nenn nne 32 Chapter 8 KINETIC On 34 85 1 Kinetic Parameter Setup nun 34 88 2 Kinetic Function Structure eenn 36 88 3 Basic Kinetic Measurement enen nnn nee 37 Chapter 9 QUANTITATIVE aana 39 89 1 Quantitative Parameter Setup enesenn nnn nne 39 89 2 Quantitative Function Structure enesenn nnn nne 40 89 3 Basic Quantitative Measurement enesenn nenn nee 41 Chapter 10 ACCESSORIES gt nun 44 810 1 Multi Cell Holder nun 44 810 2 Sipper a 46 Chapter 11 MAINTENANCE Tune 48 811 1 Routine Maintenance wenn enn nn en enennne 48 811 2 Replacement of Lamps ennenen 49 Appendix A Thermal Controller Operation o 51 Instruction Appendix B Sipper Operation Instruction _ _ 53 Appendix C Setting of USB Serial Port um 56 Metertech Inc Metertech Inc Chapter 1 INTRODUCTION The S
38. or failure can usually be attributed to simple failure of the tungsten lamp Therefore as detailed below replace the old one with a spare lamp provided before seeking further assistance 811 1 Routine Maintenance Very little maintenance is required to keep the instrument in good working condition The interior should be kept as dust free as possible and the sample compartment cleaned regularly wipe off spilt chemicals immediately CAUTION Do not allow moisture to leak into the instrument The exterior of the instrument can be cleaned periodically as follows SWITCH OFF first the instrument and disconnect from the mains supply Using a lint free cloth dampened with a weak solution of detergent and water wipe the exterior surface of the instrument as necessary Wipe over with a soft cloth dampened with clean water Dry the surface with another soft cloth 48 Metertech Inc 811 2 Replacement of Lamps This instrument is designed for easy access in lamp replacement Minimum adjustment is required for the replacement The tungsten halogen lamp is seated in a specially designed holder that is located in the right half part in the lamp compartment Figure 11 1 However the deuterium lamp that comes up with a ring typed aluminum seat is located in the left half part respectively screw screw 2 screw a screw b a
39. ories The mains electricity supply to the instrument must be disconnected at the mains supply connector and at least three minutes allowed for capacitors to discharge v The UV radiation from a deuterium lamp can be harmful to the skin and eyes Always view the lamp through protective glasses that will absorb the UV radiation and avoid looking directly at the deuterium arc Do not expose the skin to direct or reflected UV radiation v Whenever it is likely that safety protection has been impaired the instrument and or accessories must be made inoperative and secured against any unintended operation The matter should then be referred to appropriate service authority Metertech Inc Chapter 2 INSTALLATION BEFORE installation please read safety notes in previous page carefully Failing to abide by the regulations may cause damages to the instrument and may obtain incorrect results 82 1 Unpacking The SP 8001 Figure 2 1 and Figure 2 2 should be delivered with intact packaging that contains one carton with items listed in the packing list Unpack the carton and inspect carefully If any part is damaged or missing contact your local dealer immediately lamp compartment sample compartment De q fi _ Figure 2 1 Top view of the SP 8001 power receptacle RS 232 port printer port LCD contrast adjuster power switch I r C _______ Figure 2 2 Back panel of the SP 8001 Save the carton with
40. our Windows XP 98SE PC has USB ports only please install the attached USB to RS232 driver on PC Connecting the USB to RS232 cable between PC and the machine then power it up Please set up your Windows XP computer as follow Under Systems Tasks of My Computer screen press View system information In System Properties select icon Hardware and press button Device Manager figure C 1 In Device Manager double click USB Serial Port figure C 2 to enter port setting figure C 3 Please remember the com port number for further use Fill in 57600 8 None 1 None in those blanks Press button Advanced to enter advanced COM setting figure C 4 USB transfer size is to be selected as max number 4096 Latency timer is to be smallest number 1 System Properties S Eg _ System Restore Automatic Updates Remote General Computer Name Hardware Advanced Device Manager The Device Manager lists all the hardware devices installed e On MOU computer Use the Device Manager to change the properties of any device Device Manager Drivers Driver Signing lets you make sure that installed drivers are compatible with Windows Windows Update letz you set up how Windows connects to Windows Update for drivers Hardware Protiles 20 Hardware profiles provide a way for you to set up and store a different hardware configurations Hardware Profiles Figure C 1 56 Metertech Inc E
41. owing is a description of the basic procedure for carrying out measurement in the SP 8001 Kinetic measurement mode For example users want to know the reaction of a reagent in a time span of 60 36 Metertech Inc seconds after 60 second lag time by sampling 5 times at wavelength 405nm assuming that factor is 3253 and unit is set as U L Step 1 In System Main Menu select 4 then press ENTER to get into the Parameter Setup screen Step 2 In Parameter Setup screen Figure 8 2 key in required values in the corresponding parameters Each entry is followed by an ENTER If any setting needs correction use up down arrow keys to move the cursor to the proper position In item 6 select Yes followed by an ENTER key and in the unit list choose 3 U L followed by an ENTER key When all is done press F5 Next Screen to next step Step 3 Make the sample ready and press READ The measurement result will be displayed in the Kinetic screen Figure 8 3 in real time mode The measuring curve shows the measurements taken by the instrument After completion of the measurement the instrument will take the pre set number of sampling measurements for calculation to perform linear fitting The small blocks shown on the curve represent sample measurements during the rate time Those blocks are used for linear 12 00 00 KINETIC 500 0nm 0 0004 Parameter Setup 1 Samplina Number 5 2 Wavelenath nm 405 0 3 Laa Time sec 60 4 Rate Time sec 60 5 Dela
42. pressing F2 Search Peak will mark all peaks in the graph Figure 6 8 Functions F4 and F5 will let users to adjust the number of points being picked Detailed data of those points can be 28 Metertech Inc 12 00 00 SPECTRUM 800 0nm Press hot kev to processina era ety io Go Data Peak Valle Point Point Figure 6 8 Searching peaks of the spectrum viewed by pressing F1 View Data to see the wavelength and the corresponding reading Figure 6 9 SPECTRUM 800 0nm Data File List Data01 413 2nm 0 344A Data02 498 8nm 1 415A Data03 587 1nm 0 352A Data04 714 3nm 1 065A Data05 787 5nm 0 534A Page Page Data Screen Figure 6 9 Data File List for peaks 29 Metertech Inc Chapter 7 TIMESCAN This is the mode in which absorbance versus time measurement of a sample is performed for the accurate study of continuous changes in reaction rates Delay Time can be set before the start of Scan Time Results can be shown in a graph and also be manipulated in various ways as needed In System Main Menu selecting item 3 will get into this mode Instrument and analysis parameters are set up in the TIMESCAN Parameter Setup screen Figure 7 1 Move the cursor to required parameter by using arrow keys and change values of parameters Once parameters have been set press function Next Screen to get into the TIMESCAN measuring graph screen When samples are prepared ready press READ to proceed the measurement Measuring results will be s
43. re Bandwidth To perform bandwidth measurement by pressing READ This measurement detects spectrum on UV lamp and the result is used for calibration Figure 4 7 If the calibration for this instrument is correct there is a peak shown around wavelength 656 1nm and the bandwidth is less than 2nm After calibration two functions are available for users to choose Print Data for printing the results and Prev Screen for going back to the System Setup Menu screen Measure Base line To execute the base line measurement immediately after the ENTER key is pressed Search Zero Position To perform Zero Position test for hardware 17 Metertech Inc Chapter 5 PHOTOMETRIC This is a simple measurement mode with fixed wavelengths According to the parameter setup users can repeat a measurement and a table of the measurement results will be shown The results can be printed with proper settings In System Main Menu selecting item 1 will get into this mode Instrument and analysis parameters are set up in the PHOTOMETRIC Parameter Setup screen Figure 5 1 Move the cursor to required parameter by using up down arrow keys and change values of parameters Once parameters have been set press F5 Next Screen to get into the PHOTOMETRIC array typed screen When samples are prepared ready press READ to proceed the measurement Measuring results will be shown in the screen 12 00 00 PHOTOMETRIC 500 0nm Parameter Setup 1 1 nput the Number of 1 500 0
44. rly operated 1 is pump speed selection for sampling solution at low medium or high speed 2 and 4 are for air purge times setting the pump is always preset at high speed If you do not need air purge between two samplings just key in zero value it is Suggested to have air purge on during operation to remove the possible accumulated air bubble inside the cell 3 and 5 is for sample sipping time setting These two numbers added together gives the total sampling time for a complete sipping operation 6 is the delay time to read the sample after the sipping action is stopped In normal operation of the sipper a button on the sipper front panel has the same function as key READ When in the sipper parameter setup mode key READ AUTOZERO and front panel button are not active They function after leaving this mode Sometimes you do not want do measurement with sipper operated you can pick 6 system setup under system main menu Then pick 5 setup hardware then pick 2 switch to single cell The machine will operate like only one cell installed without actually removing sipper unit You can also switch it back to sipper mode by selecting NO B 3 Important notes At the first time running sipper checking the centering of flexible tube sitting on white roller of pumping wheel is very important Please enter F1 under sipper setup mode to start the pump If the tube gradually moves towards one side of w
45. shows up and the initialization of system will be automatically done The version of software coming up with the instrument is also shown in the start up screen User installed accessory will be automatically detected during the initialization 0 0nm 0 000A Metertech Metertech Inc SP 8001 UV VIS SPECTROPHOTOMETER All riahts reserved Version 1 3 Detect Accessorv Sinale Cell Initial Svstem Figure 3 5 Start up screen Following the initialization the System Main Menu screen will show up Figure 3 6 Six items representing six operation modes are available for users to select as required Users may use numerical keys or arrow keys followed by an ENTER key to complete the selection of desired mode 500 0nm 0 000A Svstem Main Menu 1 Photometric 2 Spectrum 3 Timescan 4 Kinetic 5 Quantitative 6 Svstem Setup 7 Apnplications Select Number 1 Control Figure 3 6 System Main Menu screen Two functions Quick Run and Sample Control are available in this screen Quick Run is a function the 12 Metertech Inc same as described before by using the QUICKRUN key Sample Control will be functioning only when the multi cell holder or the sipper is used 13 Metertech Inc Chapter 4 SYSTEM SETUP In System Main Menu selecting item 6 will get into the System Setup Menu screen Figure 4 1 In this screen users are allowed to alter several parameters described below In this screen only one function Lo
46. t Clear Graph Show Track Print Data Original Print Data Prev Screen Data List Switch to the KINETIC Data File List screen for users to review data obtained in previous measurements after setup of parameters Change Scale For users to perform measurements with different values of Lag Time and Rate Time Pressing this function key will switch input fields for users to key in values Measurement results can still been shown in the same KINETIC Data File List screen Repeat Measure To repeat the measurement once under the current parameter setup Linear Fit To perform linear fitting for the current measurement Show Track A vertical dotted line will show up as a tracking cursor Left right arrow keys can be used to move the cursor and the corresponding data will be displayed at the upper right of the screen In this function users press F2 Save Data to save data of the points being tracked Those data can only be used as F1 View Data or F3 Print Data being pressed and will be vanished once jumping out from this function View Data To review data being saved under function Show Track or being found under function Peak Valley Prev Page To review data in the previous page Only 15 sets of data can be displayed in one screen Next Page To review data in the next page Only 15 sets of data can be displayed in one screen Original To redraw the graph from data obtained in the current measurement 88 3 Basic Kinetic Measurement The foll
47. the Lag Time during which data will be acquired for activity calculations The value should range from 3 to 9999 For setting the time in second between pressing READ and the start of the measurement The value should range from 0 to 50 Selecting the unit option for measurement result When Yes is selected pick the right unit out of the thirteen build in units in table For entering the value of Factor This factor is used for converting the absorbance rate of change into the activity value Activity Value Factor X Abs Rate of Change ABS min The value should range from 0 to 9999 For entering the lower limit of measurement result between 0 3 and 2 99 for ABS For entering the upper limit of measurement result between 0 29 and 3 00 for ABS The High Value must be at least 0 1 greater than the Low Value Selecting temperature control over the measurement Four selections are available None 25 30 and 37 in Celsius It can be functional only when a temperature controlled accessory being installed Selecting Yes or No to overlay the current measurement with previous measurements Note that only data for the current measurement can be manipulated Selecting Yes or No to print at the end of every cycle 35 Metertech Inc 88 2 Kinetic Function Structure Kinetic Load Param Save Param Change Scale Print Data Repeat Measure Sample Control Print Data Next Screen Data List Sample Control Prev Screen Manipulate Linear Fi
48. urge operation for the previous sample The value should range from 0 to 99 9 Pre Sipper Time sec For entering the value of time during which Sipper will draw the residual of previous sample passing through the flow cell The value should range from 0 to 99 9 Air Purge Time sec For entering the value of time during which Sipper will do the air purge operation as required This usually applies to those samples that need segmentation by air The value should range from O to 99 9 Sipper Time sec For entering the value of time for the sipping operation Samples will be drawn into the flow cell for measurement essentially in this operation of the Sipper The value should range from 0 to 99 9 Dwell Time sec For entering the value of time after the sipping operation This will allow samples getting into a stable condition for measurement The value should range from 0 to 99 9 Two functions are available in this screen Wash Cell and Sipper Test Wash Cell To drive peristaltic pump running for about 15 seconds to wash flow cell whenever needed Sipper Test To execute one cycle of all operations sequentially according to the settings 47 Metertech Inc Chapter 11 MAINTENANCE The information given in this section deals only with those parts of maintenance or service which can be safely carried out by users Work other than those detailed later should be carried out by a trained service engineer Poor instrument performance
49. ursor followed by ENTER keys and the selected range of data can be zoomed Show Track A vertical dotted line will show up as a tracking cursor Left right 24 Metertech Inc arrow keys can be used to move the cursor and the corresponding data will be displayed at the upper right of the screen In this function users press Save Data to temporarily save data of the points being tracked Those data can only be used as pressing F1 View Data or F3 Print Data and will be vanished once jumping out from this function View Data To review data being saved under function Show Track or being found under function Peak Valley Prev Page To review data in the previous page Only 15 sets of data can be displayed in one screen Next Page To review data in the next page Only 15 sets of data can be displayed in one screen Peak Valley For users to find peaks or valleys in the current graph Search Peak To search peaks in the current graph Tiny squares show in the graph to represent the peaks found under this function The corresponding data can be viewed under View Data function Search Valley To search valleys in the current graph Tiny squares show in the graph to represent the valleys found under this function The corresponding data can be viewed under View Data function Less Point To reduce the number of peaks or valleys found in the current graph The corresponding data can be viewed under View Data function More Point To find more peaks or v
50. v Time sec 70 6 Have unit Yes No Yes 7 Factor default 1 3253 8 Low Value ABS 0 30 9 Hiah Value ABS 1 00 10 Measure Temperature None 11 Overlav Screen Yes 12 Print everv cvcle No Koad ood e Data somes Figure 8 2 Setting of S Seren fitting and the equation of regression is shown at the bottom of this screen T shows the rate time ABS T shows the absorbance rate of change CI T shows the concentration rate of change after conversion by using the factor In this screen users can to view the data of sampling point by pressing F1 Data List Pressing F2 Manipulate will go to next step Step 4 In this screen press F2 Linear Fit to get the regression line and it will show on the screen Figure 8 4 Here only the regression line and the sampling blocks will show up Users can also switch back to the original measuring curve by pressing F5 Original 37 Metertech Inc 12 00 00 KINETIC 405 0nm_ 0 540A 90 120 sec Factor 3253 000 AABS AT 0 11308 ABS min AC AT 367 86414 U L min ABS 0 00188T 0 27194 12 00 00 KINETIC 405 0nm 0 540A 90 120 AT 60 0 Factor 3253 000 AABS AT 0 11308 ABS min AC AT 367 86414 U L min ABS 0 00188T 0 27194 Z NOT KE D Droce NO BE a ee Fit Track Figure 8 4 Kinetic Manipulate screen Step 5 In the Kinetic Manipulate screen users can press F3 Show Track to view data of the measuring curve or the regression line by moving the dotted tracing line 38
51. y and Cell Blank Cell Home This will make the Multi Cell Holder in such a position that Cell 1 is ready for the measurement Cell Standby This will make the Multi Cell Holder in such a position that it is convenient for users to exchange cells Cell Blank not available for the time being 45 Metertech Inc 10 2 Sipper The Sipper Figure 10 3 is an optional accessory that enables samples to be drawn into a flow cell for automatic measurement After the measurement is complete the sample may be sent to a waste bottle or returned to its original vessel a Z B S From cell a P To cell 25 pin male connector Sample inlet To waste bottle Figure 10 3 Sipper A Sipper Parameter Setup screen Figure 10 4 will show up when function Sample Control being pressed Detailed settings and respective functions will be described below 12 00 00 500 0nm 0 000A Sipper Parameter Setup 1 Pump speed 2 Pre air purae time sec 3 Pre siper time sec 4 Air purae time sec 5 Sipper time sec 6 Dwell time sec press lt gt KE D Sele ae me O T e Cell Test Screen Figure 10 4 Sipper Parameter Setup screen Pump Speed Three speeds Slow Medium and Fast can be chosen by using arrow keys to set up the speed of peristaltic pump This setting only 46 Metertech Inc applies on the sipping operation for Sipper Time Pre Air Purge Time sec For entering the value of time during which Sipper will do the air p
52. y Screen is set Yes every measurement result will be displayed on the Spectrum screen Pressing F3 Clear Graph can clear all graphs in the screen Ben SPECTRUM 800 0nm 1 50 ABS 0 75 0 00 400 nm 605 800 Press READ when readv Se ham Sh Ein Data x Graph Screen Figure 6 3 Measuring result in Spectrum screen Step 5 If users want to manipulate data of results press F2 Manipulate to get into another layer of function keys Figure 6 4 Then press Fl Smooth Data and it will smooth the current graph to reduce the effect of noise To zoom data go to Step 6 to show track go to Step 7 and to search peaks and valleys of the graph go to Step 9 26 Metertech Inc 12 00 00 SPECTRUM 800 0nm Pre SCAD wnen reac se eer ey Data Data Track Valle Figure 6 4 Functions for manipulating graph Step 6 If users want to view part of the graph function Zoom Data is designed for this purpose Press F2 Zoom Data then cursor will sequentially move through 1 50 High Value 0 00 Low Value of the vertical axis and 400 Start Wavelength 800 Stop Wavelength of the horizontal axis in the graph Key in the desired value followed by an ENTER key cursor will move on to the next input position After all inputs done the instrument will display a new graph subject to the newly defined parameters Figure 6 5 12 00 00 SPECTRUM 800 0nm h t Key t Data ZE Track Valley Figure 6 5 Graph with zoomed data Step 7 If us
53. y data for the current measurement can be manipulated 8 7 2 TimeScan Function Structure TimeScan Load Param Load Data Save Param l Save Data Print Data l ccess Data Print Data Sample Control Prev Page Smooth Data View Data Next Page Next Screen anipulate Zoom Data Data Print Data Show Track Print Data Prev Screen Peak Valley View Data Prev Page Original Search Peak Next Page Clear Graph Search Valley Print Data Prev Screen Less Point Prev Screen More Point For functions not described below please refer to General Function Description section Access Data To load existing data from memory or to save data of current measurement into memory Smooth Data To smooth the data of current measurement in the screen Note that only the current measurement will go through this function and will be shown after completion of this function Zoom Data To zoom in the selected range of data in the current screen Key in proper values at blinking cursor followed by ENTER keys and the selected range of data can be zoomed Show Track A vertical dotted line will show up as a tracking cursor Right left arrow keys can be used to move the cursor and the corresponding data will be displayed at the upper right of the screen In this function users press Save Data to temporarily save data of the points 31 Metertech Inc being tracked Those data can only be used as View Data or Print Data being pressed and will be vanished once jumping out fr
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