User Manual
Contents
1. VI Hermle Labortechnik GmbH Z216M_V1 14 EG Conformity Declaration EG Konformit tserkl rung EC Conformity Declaration CE Hermle Labortechnik GmbH Siemensstr 25 D 78564 Wehingen Germany Das bezeichnete Produkt entspricht den einschl gigen grundlegenden Anforderungen der aufgef hrten EG Richtlinien und Normen Bei einer nicht mit uns abgestimmten Anderung des Produktes oder einer nicht bestimmungsgem en Anwendung verliert diese Erkl rung ihre G ltigkeit The Product named below fulfills the relevant fundamental requirements of the EC directives and standards listed In the case of unauthorized modifications to the product or an unintended use this declaration becomes invalid Produkttyp Product type Laborzentrifugen mit Zubeh r nach IVD sonstige Produkte Laboratory centrifuge with accessories to IVD other device Typenbezeichnung Typ designation Z 206 A Z 233 M 2 Z 216 M Z 306 Z 326 Z 366 Z 383 Z 400 Z 446 Z 513 Z216 Z 32 HK Z 326 K Z 366 K 236 Z 383 2 446 K 2400 Z513 K Einschl gige EG Richtlinien Normen Relevant EC directives standards 98 79 EG Anhang Annex Ill 2006 95 EG 2004 108 EG EN 61010 1 EN 61010 2 020 2007 03 EN 61010 2 101 DIN EN ISO 14971 DIN EN ISO 13485 HERMLE e Wehingen den 01 05 2014 Alexander Hermle Gesch ftsf hrer Managing Director Z216M V1 14 Hermle Labortechnik GmbH DE C Table 1 Technical Data2. HERMLE e Labnetzze Instruction Manual for Microcentrifuge Z 216 M Hermle Labortechnik GmbH Z216M V1 14 PRODUCT DESCRIPTION Senna een 1 1 1 Salely INSITUCHONS 1 1 2 Mendea 1 1 3 zb ee nennen 1 1 4 mE 1 1 5 Operating and display cl ments REI E 2 E 3 1 6 Signs and indications of the centrifuge msn 3 IF a 3 162 Prod ccnameplaue Example een 4 1 6 3 Warning and information signs nnne nennen nnn 5 1 6 4 Danger precautions anda warranty 5 1 65 Following rules must strictly be adhered to nn 5 VAN E 6 1 7 eC 7 tl oce droite ui EE Omm mmm 7 INS ME e Miei Mz BURNER Tm 7 1 7 3 MING AO 7 18 M 8 ME eid I EE 8 1 8 2 Access to mode Operating Data nnne 9 IS RETO ae de en a 10 1 84 Volume pre selection of sound signal nn 10 1 85 Song selection for sound signal end of FUN ccscececssessssssessssscsssseesessessseceeesaseceecesacaceteeesesaeeeeesasaeerersasatess 11 1 8 6 Keyboard sound turn Off nennen aeter 11 1 8 7 Call up operating data by
3. the word programm Set the display to programm M10 with the adjusting knob 1 All with number marked passages refer to figure 25 2 6 Starting and stopping the centrifuge Starting the centrifuge You can start the centrifuge either with the start key 9 or the quick key 8 By the start key 9 you can start stored runs or runs with manually pre selected parameters When the respective pre selected running time has ended then the centrifuge will stop automatically By the quick key 8 you can start runs which will last just a few seconds By pressing the quick key 8 the centrifuge accelerates up to the pre selected revolution In the display time A 3 the passed running time is indicated from the date of pressing the quick key 8 By releasing the quick key 8 the centrifuge stops and the running time is indicated until the opening of the lid All with number marked passages refer to figure 26 Z216M V1 14 Hermle Labortechnik GmbH Figure 26 2 6 2 The STOP key By the stop key 10 see figure 27 you can interrupt the run at any time After pressing the key the centrifuge decelerates with the respective pre selected intensity down to stand still Figure 27 2 7 Imbalance detection In case of the rotor not being equally loaded the drive will turn off during acceleration The rotor decelerates to stand still When in the display time A 3 the word error M11
4. together with the number 01 appear the weight difference of the samples is too huge Weigh out the samples exactly Load the rotor as described in chapter 2 1 2 and 2 1 3 When inside the display time A 3 the word error together with the number 02 see figure 28 appear there could be following reasons The imbalance switch is defective Hermle Labortechnik GmbH Z216M V1 14 21 Figure 28 22 Z216M V1 14 O Hermle Labortechnik GmbH 3 MAINTENANCE 3 1 Maintenance and cleaning 3 1 1 General Care Maintenance of the centrifuge is confined to keeping the rotor the rotor chamber and the rotor accessories clean as well as to regularly lubricating the rotor insert bolts of a swing out rotor if available Lubricants containing molycote and graphite are not allowed Please pay special attention to anodized aluminum parts Breakage of rotors can be caused even by slightest damages In case of rotors buckets or tube racks getting in touch with corrosive substances the concerned spots have to be cleaned carefully Corrosive substances are for instance alkalis alkaline soap solutions alkaline amines concentrated acids solutions containing heavy metals water free chlorinated solvents saline solutions e g salt water phenol halogenated hydrocarbons A Cleaning units rotors accessories Turn the device off and disconnect it from the power supply before you begin any cleaning or disinfecting Do n
5. 14 Hermle Labortechnik GmbH 4 2 2 Description of the error message system The error message error M11 is shown in the time A 3 display see figure 30 Detailed information about possible error messages are in table 5 error messages see Appendix P V Figure 30 O Hermle Labortechnik GmbH Z216M V1 14 27 28 5 RECEIPT OF CENTRIFUGES TO REPAIR Health risk from contaminated equipment rotors and accessories In case of returning the centrifuge for repairing to the manufacturer please notice the following The centrifuge must be decontaminated and cleaned before the shipment for the protection of persons environment and material Decontamination certificate at goods return delivery see APPENDIX P VIII We reserve the right to accept contaminated centrifuges Further on all costs occurred for the cleaning and disinfection of the units will go to the debit of the customer s account Z216M V1 14 Hermle Labortechnik GmbH 6 TRANSPORT STORAGE AND DISPOSAL 6 1 Transport Before transporting take out the rotor Only transport the unit in the original packaging Use a transport aid for transporting over longer distances to fix the motor shaft 25 bis 60 C 10bis 75 30 bis 106 kPa 6 2 Storage During storage of the centrifuge the following environmental conditions must be observed in transport packaging 25 bis 55 C 10 bis 75 96 70 bis 106 kPa 6 3 Disposal In the
6. 2 Open the rotor nut by turning the rotor key counter clockwise 3 Remove the rotor 4 For cleaning and disinfection of the unit and the rotor chamber using the above mentioned cleaner 5 Clean all accessible areas of the device and its accessories including the power cord with a damp cloth 6 Wash the rubber seals and rotor chamber thoroughly with water 7 Rub the dry rubber seals with glycerol or talc to prevent these to becoming brittle Other components of the unit e g the lid lock motor shaft and rotor must not be greased 8 Drythe motor shaft with a soft dry and lint free cloth 9 Control the unit and accessories for damage Remove at least every six months adherent dust from the ventilation slots in the centrifuge by using a soft brush Before doing that please switch off the unit and disconnect the unit from the power supply 3 1 3 Cleaning and disinfection of the rotor Clean and disinfect the rotors rotor lids and adapters with the above mentioned cleaner Use a bottle brush to clean and disinfect the rotor bores Rinse the rotors rotor lid and adapter with clear water Particular the drillings of angle rotors For drying of the rotors and accessories set them on a towel Place the angle rotors with bores down to dry them to 5 Dry the rotor cone with a soft dry and lint free cloth and look for damage Do not grease the rotor cone 3 1 4 Disinfection of aluminum rotors In case of in
7. M6 rof M3 hms M7 accel 16 Signs and indications of the centrifuge 1 6 1 General Instructions for disposal see 29 gt Direction of rotation clockwise rotation for the rotor drive 63 Reference for loading rotors Hermle Labortechnik GmbH Z216M V 14 3 Company address TYPE REF SN MAX Drehzahl KIN EN an 1 6 2 Product nameplate Example Hermle Labortechnik GmbH Siemensstr 25 D 78564 Wehingen TYPE Z36HK REF 302 00 V01 SN 58105001 r 2010 MAX DREHZAHL 30000 1 min KIN EN 50880 Nm 120 15 8A 50 60Hz 1 6KW ce Made in Germany C Hermle Labortechnik GmbH Siemensstr 25 D 78564 Wehingen Type designation of the product Order no of the product Serial number of the product Date of manufacture max allowed speed of the unit max kinetic energy with corresponding rotor Allowable voltage max current frequency Electrical input power Before operating read the operating manual Labeling standards and guidelines that are considered Z216M V1 14 Hermle Labortechnik GmbH 1 6 3 Warning and information signs Warning Four carrier must be used at ali Four carrier must be used at all times on four place swing out rotors or nn damage will occur to the centrifuge Such damage will not be covered rotors or damage will occur to the centrifuge Such damage will not be under the
8. adapters and rotors may be treated with the usual liquid disinfectants ATTENTION Before applying any other cleaning resp Decontamination method than recommended by the manufacturer contact the manufacturer to ensure that it will not damage the unit or the rotor 3 1 6 Glass breakage With high g values the rate of glass tube breakage increases Glass splinters have to be removed immediately from rotor buckets adapters and the rotor chamber itself Fine glass splinters will scratch and therefore damage the protective surface coating of a rotor If glass splinters remain in the rotor chamber fine metal dust will build up due to air circulation This very fine black metal dust will extremely pollute the rotor chamber the rotor the buckets and the samples If necessary replace the adapters tubes and accessories to avoid further damages Check the rotor bores regularly for residues and damages ATTENTION Please check the relevant specifications of the tubes centrifuges with the manufacturer 3 2 Life time of rotors round and rectangular buckets accessories Rotors and rotor lid made of aluminum or stainless steel have a operating time of max 7 years from first use Transparent rotor lids and caps made of PC or PP as well as rotors tube racks and adapters of PP have a maximum operating time up to 3 years from first use Condition for the operating time Proper use damage free condition recommended care Hermle Labortec
9. product warranty covered under the product warranty Attentian Check the fastening of the rator nut before each run Achtung Attention Check the fastening of the rotor nut before each run Vor jedem Lauf Befesti gungsschraube auf festen Sitz pruefen Vor manueller Entriegelung oder ffnen des Geh uses Netzstecker Ziehen TAKE OFF MAINS PLUG before opening Take off mains plug before opening the housing or the emergency release the housing or the emergency release RETIREZ LE CORDON avant toute intervention a l interieur de l appareil 1 6 4 Danger precautions and warranty A This device may only be operated by trained specialist stuff They must have carefully read the operating manual and be familiar with the function of the device To protect people and environment the following precautions should be observed During centrifugation the presence of people and the setting up of hazardous materials is prohibited within 30 cm around the centrifuge according to the regulations of EN 61010 2 020 The HERMLE Z 216 M is non explosion proof and must therefore not be operated in explosion endangered areas or locations Centrifugation of flammable explosive radioactive or such substances which chemically react with high energy is strictly prohibited The final decision on the risks associated with the use of such substances is the responsibility of the user of the centrifuge Never spin toxic or pathogenic m
10. same time and hold them In the display time A 3 appears the word radius M9 By the adjusting knob 1 you can preselect then the respective radius correction see table 6 APPENDIX P VI in steps of 0 1 cm As soon as you have set a radius correction the word radius M9 appears This hint is as long visible as you put the radius correction back to 0 again All with number marked passages refer to figure 21 Figure 21 2 5 Program 2 5 1 Storage of programs You can store up to 99 runs with all relevant parameters incl the used rotors You can use any free program number and call it up again Put the needed rotor into the centrifuge By pressing the key prog 11 in the display time A 3 appears the word program see figure 22 With the adjusting knob 1 you can chose the desired program number If a program number is already occupied in the display rpm rcf 1 will appear the words rotor and 22 M4 see figure 22 In case of free program numbers it appears 0 Figure 22 Z216M V1 14 Hermle Labortechnik GmbH Close the lid of the centrifuge Now proceed as already described to set all important run parameters If the lid isn t closed when storing the programm in the display rpm rcf 1 flashes alternately the word FirSt and CLOSE Lid see figure 23 If you want to start the run without storing the programm in the display rpm rcf 1 fl
11. skilled or service engineer only seen 12 2 OPERATION e M 13 2 1 Mounting and loading angle rotor M 13 VAN TOOS a a sad 13 2 1 2 Loading angle rotors RINT een 13 2 1 3 Loading ANG overloading Of rotors nennen aa 14 ZR REON LOTS 14 2 2 IE o 15 ETO E E 15 OR ne 15 2 3 gini e 16 2 3 1 Preselecuoror speed HOPSIalUe nee 16 252 Preselection of running 16 2 3 3 Preselection of brake intensity and acceleration 17 2 4 Radius COFTOCTION 18 2 5 mee 18 291 18 252 RecallOlSsiored LOOT AIS nn 20 Hermle Labortechnik GmbH Z216M V1 14 238 MES NG ONO CT AM ee rer 20 2 6 Starting and stopping the CENIFITUQR nets nn 20 261 een 20 ee 21 2 7 Imbalange u a 21 MAINTENANCE rennen 23 3 1 Maintenance and 23 ee 23 3 1 2 Cleaning and disinfection of the unit 23 3 1 3 Cleaning and disinfection Of ann
12. word store in the display rpm rcf A 1 Only then the pre selections are valid see figure 4 Figure 4 Hermle Labortechnik GmbH Z216M V 14 10 1 8 3 Signal turn on off optional Proceed as described under point 1 8 2 to enter this program mode and then press the key accel decel 5 In the display accel decel 2 flashes the word service Now select the letter L with the adjusting knob 1 As a result appear in the display rpm rcf 4 the words On Sound If you press the key rpm rcf 4 now the word On flashes and you can switch off the sound with the adjusting knob 1 see figure 5 After you have stored the settings see 1 8 2 you changed to the normal program mode again by switch off the centrifuge for a short while Figure 5 Volume pre selection of sound signal optional Proceed as described under point 1 8 2 to enter this program mode and then press the key accel decel 5 In the display accel decel 2 flashes the word service Now select the letter U with the adjusting knob 1 As a result appear in the display rpm rcf 1 the words Vol 0 9 Sound After pressing the key rpm rcf 4 you can adjust the desired volume between 0 low and 9 loud with the adjusting knob 1 see figure 6 After you have stored the settings see 1 8 2 you changed to the normal program mode again by switch off the centri
13. Manfacturer HERMLE Labortechnik GmbH Type Z 216 M Dimensions Width Depth Height max speed 15000 min max volume 44 x 1 5 2 ml max RCF 21380 x g allowable density 1 2 kg dm allowable kinetic energy 7060 Nm Mains power connection AC 230 V 50 60 Hz 1 ph 120 V 50 60 Hz 1 ph Voltage fluctation 10 10 Current consumption 1 3A 2 3A Power consumption 270 W 280 W Radio interference IEC 61326 1 Audit requirement BGR 500 no Ambient conditions EN IEC 61010 1 Environement High Ambient temperature for indoor use only Use up to an altitude of 2000 m above MSL 2 C up to 35 C Max relative humidity Max relative humidity 80 9o for temperatures up to 31 C decreasing linearly to 50 relative humidity up to 35 C 2 Overvoltage category IEC 60364 4 443 Degree of contamination Not suitable for use in hazardous environements EMV EN IEC FCC Class B EN IEC FCC Class B Interference emission noise 61326 1 61326 1 Category B Category B Noise level depending on the rotor lt 60 2 dB A Write from operator Inventory No Monitoring No Environement Maintenance contract Siemenssirasse2s _ Te 974267862207 OOOO 245 responsible dealer Hermle Labortechnik GmbH Z216M V1 14 220 87 V14 220 87 V13 221 17 V06 220 88 V08 221 35 V03 220 92 V07 221 38 V03 220 58 V13 Table 2 Permissible net weight 15000 mi
14. Over current frequency converter Timeout between control unit and frequency converter Other error frequency converter N Timeout between controler and RS232 interface 33 98 _ FALSE Inserted rotor does not exist in the programm Hermle Labortechnik GmbH Z216M V1 14 Table 6 part 1 Radius correction Rotor No Adapter Tuberack Order no cm Angle rotor o ele 220 87 Omoa oa aa Angle rotor Lum 7005 84 40 _ _ a rotor 704 004 704 005 F non rotor Angle rotor Angle rotor 701 015 701 017 Table 7 Abbreviations used Symbol Abbreviations U m m rp in revolutions per minute RZB rcf rc x gl relative centrifugal force Polypropylen Polyca deceleration program VI Z216M V1 14 Hermle Labortechnik GmbH Redemption form Decontamination certificate Decontamination certificate at goods return delivery Enclose at all returns of equipment and assemblies absolutely The completely full declaration about the decontamination is prerequisite for the assumption and further processing of the return If no corresponding explanation is enclosed we carry out decontamination with costs at your expense Surname last name xX Organization company 9 Street 8 ZIP CODE place pum Telephone fax E Mail Crowd Decontaminated Description Comment object Are these pa
15. ashes alternately the word First and PrESS StoreE see figure 24 Figure 23 Figure 24 For adaption of data press the key store 12 for approx 1 second If the programm is stored correctly the word StorE appears in the display 1 As a result the word program M10 disappears As soon as the key store 12 is no longer anymore it reappears the word programm xx M10 the xx stands for the chosen program place If all program numbers are occupied you can take an old number that is not necessary anymore and just put in the new parameters Hermle Labortechnik GmbH Z216M V1 14 19 20 2 5 2 2 5 3 2 6 1 Recall of stored programs To recall stored programs press the key prog 11 while the lid is already closed Inside the display time appears programm M10 With the adjusting knob 1 you pre select the desired program number In the respective displays there will appear the stored values for that program If there is not the right rotor inside the centrifuge for the pre selected program in the display rom rcf 1 flashes the word rotor At the same time the word FALSE and the stored rotor number 22x xx M4 will flashing by turns All with number marked passages refer to figure 25 Figure 25 Leaving program mode To leave the program mode just press the key prog 11 Then inside the display time appears
16. aterial without adequate safety precautions i e centrifugation of buckets tubes without or with defective hermetic sealings is strictly prohibited The user is obliged to perform appropriate disinfection procedures in case dangerous substances have contaminated the centrifuge and or its accessories When centrifuging infectious substances always pay attention to the General Laboratory Precautions If necessary contact your safety officer It is prohibited to run the centrifuge with rotors other than listed for this unit Under no circumstances open the lid of the centrifuge while the rotor is still running or rotating with a speed of 2m s 1 6 5 Following rules must strictly be adhered to Hermle Labortechnik GmbH Do not operate the centrifuge in case it is not installed correctly Do not operate the centrifuge when dismounted e g without housing Z216M V 14 Do not run the centrifuge when mechanical or electrical assembly groups have been tampered with unauthorized persons Do not use accessories such as rotors and buckets which are not exclusively approved by HERMLE Labortechnik GmbH except commercially available centrifuge tubes made of glass or plastic Do not spin extremely corrosive substances as they may cause material damages and impair mechanical resistance Do not operate the centrifuge with rotors or buckets which show any signs of corrosion or mechanical damage The manufacturer is res
17. e 24 3 1 4 Disinfection of aluminum rotorS usesseeseeneeneeneenennennennennennnnnnnnennnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnsnnnnsnnsnnsnnnnnnnnnnsensenenn 24 SNEOMEEP riunioni Nm 24 3 60 lass DIE RETO mmm 25 3 2 Life time of rotors round and rectangular buckets accessories eese 25 4 TROUBLE 5 26 4 1 Error message Cause Eli m 26 4 2 Survey of possible error messages and their solutions nennen 26 4 2 1 Lid release during power failure Emergency Lid Release 26 4 2 2 Description of the error message system nn 27 5 RECEIPT OF CENTRIFUGES TO REPAIR eere 20 6 TRANSPORT STORAGE AND DISPOSAL eene 29 6 1 Dn 29 SIDA aq 29 6 3 DISHOS an een ee 29 l APFPENDIA qe EG Conort Decard OR OON TET A na ea a ne or nai ee E Table 2 Permissible net weight inner IV Table 3 Max speed and RCF values for permissible rotors cccccccsssssescscsssssssssscssssssescessessessssssssseeeseseseseeesesesess IV Table 4 Acceleration and deceleration times ss IV D ee V Table 6 part 1 Radius correction VI Table 7 Abbreviations VI Redemption form Decontaminati
18. e key stop 10 The time countdown as soon as the set speed is reached The display shows always the remaining running time see figure 19 All with number marked passages refer to figure 19 Figure 19 Preselection of brake intensity and acceleration Through the key accel decel 5 this function is activated By pressing the key once the word accel M7 flashes in the display accel decel 2 The desired acceleration can be pre selected by the adjusting knob 1 The value 0 is equivalent to the lowest and the value 9 to the highest acceleration By pressing the key accel decel 5 twice in the display accel decel A 2 indicates the word decel M8 Now the desired brake intensity can be pre selected by the adjusting knob 1 The value 9 is equivalent to the shortest and the value 0 to longest possible brake time All with number marked passages refer to figure 20 See table 4 acceleration and deceleration times APPENDIX P IV There are shown the acceleration and deceleration times for the acceleration and deceleration stages 0 to 9 for permissible rotors Abbildung 20 O Hermle Labortechnik GmbH Z216M V1 14 17 18 2 4 Radius correction If you use adapters or reducers it could change the centrifugal radius of the respective rotor In that case you can correct the radius manually Please proceed as follows Press the key time 6 and the key prog 11 at the
19. e label on rotor must not be exceeded The liquids the rotors are loaded with should have an max homogeneous density of 1 2 g ml or less when the rotor is running at maximum speed In order to spin liquids with a higher density the speed has to be reduced according to the following formula 1 2 Reduced speed Porc x speed nmax of the rotor Example 1 2 Nred 2 4 000 3 360 rpm If In case of any questions please contact the manufacturer Removing the rotor Untighten the rotor fixing nut complete and lift the rotor vertical out of the centrifuge see figure 11 Z216M V1 14 Hermle Labortechnik GmbH 2 2 Lid 2 2 1 2 2 2 Lid release After the run respectively closing the lid of the centrifuge it appears in the display rpm rcf A 1 the word close M1 If there is a rotor in the centrifuge it appears additional the word rotor as well as the code number of the respective rotor which is in the centrifuge i e 220 72 M4 If there is no rotor in the centrifuge it flashes the word rotor and additional the word M4 By pressing the key lid 7 you can release the lid of centrifuge As soon as the motor driven lid lock is completely released it appears the word open M2 Now you can open the lid of the centrifuge All with number marked passages refer to figure 17 Figure 17 During the run you can call up the rotor type at any
20. ed with angle rotors All parameters are accessible via buttons and selected with the central adjuster All pre selected and current values will be shown permanently on the LCD display The centrifuge is powered by a maintenance free induction motor Detailed technical data are in table 1 Technical data see APPENDIX 5 111 1 4 Delivery package e 1 Centrifuge Z 216 M e 1 Operating Manual Z 216 M e 1 Rotor key Rotor s Accessories will be packed separate Hermle Labortechnik GmbH Z216M V 14 1 5 Operating and display elements cO OO N O N D central adjuster run parameters 0 1 LCD rpm rcf accel decel time lid quick start stop prog store power switch control panel display speed g force acceleration deceleration intensity centrifugation time lid release short running start centrifugation stop centrifugation calling stored programs program store Z216M V1 14 Hermle Labortechnik GmbH 1 5 1 LCD Display The following picture shows the individual elements of the LCD display M1 2 M8 M7 M10 M11 M9 Figure 1 Display fields A 1 Display field rom rcf A 2 Display field acc dec A 3 Display field time Messages logos of the display fields M1 close M8 M2 open M9 radius M3 rotor M10 program M4 Rotor No M11 error M5 rpm M12 service
21. event of disposing of the product please observe the applicable legal regulations Information on the disposal of the electrical and electronic devices in the European Community The disposal of the electrical devices is regulated within the European Community by national regulations based on EU Directive 2002 96 EC pertaining to waste electrical and electronic equipment WEEE In accordance with this any devices delivered after 13 08 2005 on a business to business basis which includes the product may no longer be disposed of in household waste To document this they have been marked with the following identification Because disposal regulations may differ from one country to another within the EU please contact your supplier if necessary Hermle Labortechnik GmbH Z216M V1 14 29 7 EG Conformity Declaration nn TECHNICAL DAA a a a a ia it Table 2 Permissible net weight nn IV Table 3 Max speed and RCF values for permissible rotors IV Table 4 Acceleration and deceleration times IV Table 5 Error messages 4 eese eee ee enne nena tna nn anna ana tna sn asa a anas V Table 6 part 1 Radius correction eren nnns VI Table 7 Abbreviations used M VI Redemption form Decontamination certificate
22. fectious material spilling into the centrifuge the rotor and rotor chamber have to be disinfected right after the run Rotors may be autoclaved at a maximum temperature of 121 C 3 1 5 Disinfection of PP rotors Autoclaving The recommended time for autoclaving 15 20 min at 121 C 1 bar ATTENTION The sterilization time of 20 min must not Sterilization again and again will cause reduction of the mechanical resistance of the plastic material Before the autoclaving the PP rotor and adapter must thoroughly be cleaned to avoid the burning in of dirty residues You can disregard the consequences of some chemical residues to plastic materials at ambient temperatures But at the high temperatures of the autoclaving those residues may corrode and destroy the plastic The objects must be thoroughly washed up with distilled water after the cleaning but before the autoclaving Residues of any cleaning liquids may cause fissures whitening and stains Gas sterilization 24 Z216M V1 14 O Hermle Labortechnik GmbH Adapters bottles and rotors may be gas sterilized with Ethylenoxyd According to the duration of the application you may give long enough an airing to the items after the sterilization and before using them again ATTENTION Because the temperature may rise during the sterilization rotors adapters and bottles must not be closed respectively must be totally unscrewed Chemical sterilization Bottles
23. fuge for a short while rpmircf Figure 6 Z216M V1 14 Hermle Labortechnik GmbH 1 8 5 Song selection for sound signal end of run optional Proceed as described under point 1 8 2 to enter this program mode and then press the key accel decel 5 In the display 2 flashes the word service Now select the letter Q with the adjusting knob 1 As a result appears in the display rpm rcf 1 the word SonGo Sound After pressing the key rpm rcf 4 you can select a song with the adjusting knob 1 see figure 7 After you have stored the settings see 1 8 2 you changed to the normal program mode again by switch off the centrifuge for a short while rpm rcf Figure 7 Keyboard sound turn on off optional Proceed as described under point 1 8 2 to enter this program mode and then press the key accel decel 5 In the display accel decel 2 flashes the word service Now select the letter with the adjusting knob 1 As a result appears in the display rom rcf 1 the word After pressing the key rpm rcf 4 you can turn the keyboard sound On or Off with the adjusting knob 1 see figure 8 After you have stored the settings you changed to the normal program mode again by switch off the centrifuge for a short while rpm rcf Figure 8 Hermle Labortechnik GmbH Z216M V 14 1 8 7 Call u
24. hnik GmbH Z216M V1 14 25 4 TROUBLE SHOOTING 4 1 Error message Cause Solution The error messages are listed to help localize possible errors faster The diagnose referred to this chapter may not always be the case as they are only theoretically occurring errors and solutions Always please keep us informed about any kind of error occurring which is not listed in this chapter Only through your information we are able to improve and complete this operation manual Many thanks in advance for your support HERMLE Labortechnik GmbH 4 2 Survey of possible error messages and their solutions 4 2 1 Lid release during power failure Emergency Lid Release In case of power failure or malfunction the lid of the centrifuge can be opened manually in order to protect your samples Please proceed as follows see figure 29 Switch the centrifuge off and unplug the power cord wait until the rotor stands still this may take several minutes e At the left side of the centrifuge housing there is a plastic stopper Remove this stopper and behind it there is a hexagon nut Take the delivered box spanner put him in the hole and lock the box spanner with hexagon nut see figure 29 Nowturn the box spanner to the right side clockwise up to the limit ATTENTION Just turn to the limit don t tighten the nut Now open the lid of the centrifuge e Switch the centrifuge on again for go on working Figure 29 26 Z216M V1
25. le the used rotor now see figure 2 To adopt the data in the unit please press the start 9 key Inside the display A 1 the stroke store appears to the confirmation Now all important rotor parameters for the centrifuge are stored Figure 2 Attention Set rotor must always match with the used rotor otherwise you might damage the unit The rotor type can be checked during the run by pressing the key lid 7 At commissioning of the centrifuge you have the options to make the following basic settings Acoustic signal turn on off Keyboard sound turn on off Volume pre selection of sound signal Song selection of sound signal end of run Z216M V1 14 Hermle Labortechnik GmbH 1 8 2 Access to mode Operating Data If the centrifuge is still turned off press simultaneously the keys time 6 and lid 7 and turn on the main switch of the centrifuge Now release both keys again As a result a display test is executed for approx 5 seconds All possible indications will appear at the same time see figure 3 Figure 3 ATTENTION Please notice that you must enter the program as described under point 1 8 2 to change the adjustments of the points 1 8 3 1 8 7 After you have stored the settings you change the normal program mode again by switch off the centrifuge for a short while changed settings must be confirmed by the key start 9 As an optical confirmation appears the
26. n 1 15000 min 1 13500 min 1 13500 min 1 14500 min 1 13500 min 1 15000 min 1 12000 min 1 permissible weight Table 3 Max speed and RCF values for permissible rotors Rotor Number max speed permissible weight 220 87 V14 15000 min 1 21380xg 220 87 V13 15000 min 1 21380x g 221 17 V06 13500 min 1 19153xg 220 88 V08 13500 min 1 17113xg 221 35 V03 14500 min 1 15342xg 220 92 V07 13500 min 1 16298xg 221 38 V03 15000 min 1 15342xg 220 58 V13 12000 min 1 14970xg Table 4 Acceleration and deceleration times Acceleration values Deceleration Rotor Number level 0 9 level 0 9 220 87 V14 220 87 V13 221 17 V06 220 88 V08 221 35 V03 220 92 V07 221 38 V03 220 58 V13 in seconds Acceleration time Deceleration time 1 from Umax gt min 1 from min gt Umax IV Z216M V1 14 Hermle Labortechnik GmbH Table 5 Error messages Description Imbalance arose Imbalance sensor is defective Transponder in the rotor is defective 14 Leap of speed is too big between two mesaurements CLOSE lid pen lid while motor is running id contact defective id motor is blocked ommunication with frequency converter distrubed during start Communication with frequency converter distrubed during stop hort circuit in the frequency converter Undervoltage frequency converter Overvoltage frequency converter Over temperature frequency converter Over temperature motor N
27. on certificate LR VI Z216M V1 14 Hermle Labortechnik GmbH 1 PRODUCT DESCRIPTION 1 1 Safety instructions This symbol indicates safety instructions and points to potential dangerous situations Before using the centrifuge the first time please read the operating manual Failure to follow these instructions can result in personal injury an property damage Intended use includes the observance of all instructions in the instruction manual and carrying out inspection and maintenance 1 2 Intended purpose This Hermle centrifuge was designed only for the separation of materials or mixtures with different density specifically for the preparation and processing of samples from the human body in context of an in vitro diagnostic use to allow the use of in vitro diagnostic in accordance to its intended purpose The designated device and its accessories listed in the technical documentation for this device are in accordance with Directive 98 79 EC on in vitro diagnostic medical devices Hermle centrifuges are intended exclusively for indoor use and for use by qualified personnel Only Hermle original rotors and accessories might be used Any other use or intended use is considered improper From the resulting damage the company Hermle Labortechnik is not liable 1 3 Brief Description The unit type Z 216 M is a microlitre centrifuge which we offer in two voltage variations 230V or 120V The centrifuge can be us
28. or in the centrifuge the speed can only be pre selected until the maximum permissible revolution of that rotor It is the same with the pre selection of the RCF value The setting range is between 20 xg and the maximum permissible centrifugal force of the rotor See table 3 max speed and RCF values for permissible rotor see APPENDIX P IV There are listed all important values ATTENTION Please also check the maximum permissible revolutions of your test tubes Producer Indication Preselection of running time The running time can be pre selected in three different ranges from 10 seconds up to 99 hours 59 minutes 1 Range from 10 seconds up to 59 minutes 50 seconds in steps of 10 seconds 2 Range from 1 hour up to 99 hours 59 minutes in steps of 1 minutes 3 Range continuous run cont which can be interrupted by the key stop 10 The running time can be pre selected with the lid open or closed To activate the setting of the running time press the key time 6 Z216M V1 14 Hermle Labortechnik GmbH 2 3 3 In the display time A 3 flashes the indication m s or h m depending on the previous setting To set the desired value use the adjusting knob 1 After exceeding of 59 min 50 sec the indication changes automatically into h m After exceeding of 99 hours 59 min the word appears in the display time A 3 That continuous run can only be interrupted by pressing th
29. ot pour liquids into the housing interior Do spray disinfectant on the device Thorough cleaning not only has its purpose in hygiene but also in avoiding corrosion based on pollution In order to avoid damaging anodized parts such as rotors reduction plates etc only pH neutral Detergents with a pH value of 6 8 may be used for cleaning Alkaline cleaning agents pH value gt 8 must not be used After cleaning please ensure all parts are dried thoroughly either by hand or in a hot air cabinet max Temperature 50 C It is necessary to coat anodized aluminium parts with anti corrosion oil regularly in order to increase their life spans and reduce corrosion predisposition Due to humidity or not hermetically sealed samples condensate may be formed The condensate has to be removed from the rotor chamber with a soft cloth regularly A The maintenance procedure has to be repeated every 10 to 15 runs but at least once a week Connect the unit to the power supply after the equipment is completely dry Do not carry out disinfection with UV beta and gamma rays or other high energy radiation Metal rotors can be autoclaved Rotor lid and adapters can also be autoclaved max 121 C 20 min The tube racks are made of PP and cannot be autoclaved at 134 C 3 1 2 Cleaning and disinfection of the unit 1 Open the lid before you turn off the unit Disconnect it from the power supply Hermle Labortechnik GmbH Z216M V1 14 23
30. p operating data by skilled or service engineer only In tne mode Basic Adjustments you can call up the operating data of the centrifuge Please proceed as described under point 1 8 2 to enter this program mode Press the key accel decel 5 In the display accel decel A 2 flashes the word service With the adjusting knob 1 the different information can be called up A previous starts of the centrifuge H previous operating hours 5 software version r Converter software E list of previous error massage h running time of the motor The list of the last 99 error messages can be looked over by pressing the key rpm rcf 4 and scroll through it by the adjusting knob 1 The respective error codes appear in the display rpm rcf 1 Please look up in Table 5 error messages see APPENDIX 5 V Here as well you must shortly switch off the centrifuge for changing to the normal program mode again rpm rcf Figure 9 Z216M V1 14 Hermle Labortechnik GmbH 2 OPERATION 2 1 Mounting and loading angle rotor 2 1 1 2 1 2 Installation of rotors Clean the drive shaft as well as the rotorshaft with a clean grease free piece of cloth Place the rotor onto the drive shaft see figure 10 Take care that the rotor is fully installed onto the motor shaft 10 11 Hold the rotor with one hand and secure the rotor to the shaft by turning the fi
31. ponsible for safety and reliability of the centrifuge only if the unit is operated in accordance with this instruction manual modifications repairs or other adjustments are performed by HERMLE authorized personnel and the electrical installation of the related location corresponds to the IEC regulations 1 6 6 Warranty The centrifuge has been subjected to thorough testing and quality controls In the unlikely case of any manufacturing faults occurring the centrifuge and rotors are covered by warranty for a period of two years from date of delivery This warranty becomes invalid in case of mishandling damage and negligence and further in case of usage of inappropriate spare parts and or accessories or unauthorized modification of the unit Technical modification rights are reserved by the manufacturer in respect to technical improvement Z216M_V1 14 Hermle Labortechnik GmbH 1 7 Installation of the centrifuge 1 7 1 Unpacking the centrifuge Model Z 216 M is supplied in a carton Remove the strap retainer open the carton and remove the centrifuge The instruction manual must always be kept with the centrifuge 1 7 3 Space requirements The centrifuge should be installed on an even solid surface if possible on a laboratory cabinet table or some other solid vibration free surface During centrifugation the centrifuge must be placed in a way that there is a minimum space of 30 cm on each side of the unit according
32. rts listed above in touch with the following substances Health endangering watery solutions buffers acids alkalis O Yes No Potentially infectious agents enn Yes Organic reagents and solvent rre O Yes O Radioactive substances 0 y O Yes No Health endangering proteins un O Yes BuU Yes These substances have reached the equipment assembly Yes Which one if yes Description of the measures for the decontamination of the listed parts confirm the proper decontamination Company dept place and date Signature of the authorized person Hermle Labortechnik GmbH Z216M V1 14 VII Labnet International Inc 31 Mayfield Ave Edison 08837 USA US toll free 888 LABNET 1 fax 732 417 1750 International phone 1 732 417 0700 fax 1 732 417 1750 tp www labnetinternational com Website ht Email labnetinfo corning com Technical modification rights reserved HERMLE Labortechnik GmbH 2014 Hermle Labortechnik GmbH Z216M V1 14
33. time by pressing the key lid 7 Lid lock The lid must only be lay down slightly A motor driven lid lock closes the lid at the same time disappears the word open M2 As a sign that the centrifuge is ready for starting it appears in the display rpm rcf A 1 the word close M1 Simultaneously it appears in that display the word rotor M3 as well as the code number of the rotor which is in the centrifuge i nr 22x xx M4 With that all rotor specifically data like e g max speed acceleration etc are adopted All with number marked passages refer to figure 17 ATTENTION Don t grip your fingers between lid and device locking mechanism when closing the lid Hermle Labortechnik GmbH Z216M V1 14 2 3 Preselection 2 3 1 2 3 2 Preselection of speed RCF value Through the key rpm rcf 4 this pre selection is activated By pressing the key once the word rpm M5 flashes By pressing the key once again the pre selection of the centrifugal forces may be chosen Then it appears the flashing word ref M6 You can set the desired values with the adjusting knob 1 In the display A 1 the regulated value is shown permanently before during and after the run All with number marked passages refer to figure 18 mo Figure 18 As long as no rotor is inserted the speed is adjustable between 200 rpm and maximum revolution of the centrifuge If there is a rot
34. to the standards EN 61010 2 020 Do not place the centrifuge next to a window or a heater where it could be disposed to excessive heat as the performance of the unit is based on an ambient temperature of 23 C 1 7 3 Installation Follow these steps e Check whether power supply corresponds with the one named on the manufacturer s rating label which is mounted on the rear panel The line voltage circuit braker is max 10 A type K slow release for commonly used instruments n case of emergency there must be an emergency switch off installed outside the room in order to disconnect the power supply of the unit Connect the centrifuge with the mains The socket for the power cord must be easy to reach respectively easy to disconnect Switching it on using the mains power switch 1 Open the lid by using the button LID Remove the transport securing device of the motor Hermle Labortechnik GmbH Z216M V 14 1 8 Grundeinstellung 1 8 1 Adjustment of the rotortype Before the first operation resp after each rotor change you have to settle the respective rotor type You find each rotor type in the printed order number on the rotor Example Angle rotor 220 87 V11 rotortype 87 Switch on the unit and open the lid Now press simultaneously the keys lid 7 and stop 10 In the display rpm rcf 1 then appears the old settled rotor type i e 96 With the potentiometer 1 you can sett
35. xing nut clockwise Tighten fixing nut with enclosed allen key see figure 11 Figure 12 ATTENTION For safety always ensure that rotor fixing screw is tightened before each run see figure 11 Loading angle rotors Rotors must be load symmetrically and with equal weight see figure 13 14 The adapter may only be load with the appropriate vessels The weight differences between the filled vessels are as low as possible to keep Therefore we recommend to weighting with a balance This reduces the wear of drive and the acoustic operating noise On each rotor is designated how large the maximum load per hole is It is allowed to operate e g a 12 place rotor with 2 or 4 loaded tubes only But the loaded borings must be opposite each other Hermle Labortechnik GmbH Z216M V1 14 13 Figure 13 CORRECT Figure 14 WRONG A ATTENTION 2 1 3 2 1 4 Do not operate the centrifuge with rotors or buckets which show any signs of corrosion or mechanical damage Do not operate with extremely corrosive substances which could damage the rotor and buckets In case of any questions please contact the manufacturer Loading and overloading of rotors All approved rotors are listed with their maximum speed and maximum filling weight in table 2 permissible net weight see APPENDIX P IV The maximum load permitted for a rotor which is determined by the manufacturer as well as the maximum speed allowed for this rotor se
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