Twinkle - Phenix Research Products
Contents
1. user tries to run the workstation This is because during the installation some of the registry files written to the hard disk will be accessible only for the one that was logged in during the installation To overcome this problem there are three different possibilities Install the workstation with the PC disconnected from the net This is not the best possible way to do it since depending on how the net is constructed there might still be problems with the registry files for the different users Also the icons for starting the workstation might be missing Make a separate installation for each user Install the workstation for each user separately logged into the PC Allthe necessary files and icons will be accessible for each one If there are many users this is time consuming Make a separate user name for the fluorometer users The local net support person creates a single user name and password specially for the fluorometer users The installation is then done when logged in as this user 16 Setup Exit all Windows programs and insert the CD ROM into the PC the setup will start automatically Note If the setup does not start automatically it is probably because the Auto insert notification for the CD ROM drive is not activated Run the setup exe on the CD or activate the auto insert notification in the following way My computer gt Control panel gt System gt Device Manager gt Double Click CD ROM gt Double Click CD ROM2. 30 nm Detector State of the art low noise high sensitivity photon counting system sensitive in the range of 300 to 650 nm Plates 1 to 864 well plates are compatible with the instrument The user can define non standard plate configurations The maximum outer dimensions are 86 0 x 128 2 x 25 mm Scanning Scanning of wells several measuring points within a well is available 21 Repeats Repeated measurements or operations can be defined by plate strip or well Measurement time Detection time per well is software selectable between 0 1 600 seconds Performance Fluorescein detection limit is lt 10 fmol well Physical dimensions Weight 17 kg Height 280 mm Width 465 mm Depth 406 mm Software The instrument program is run under Windows 95 Windows 98 or Windows NT 4 0 on a Pentium computer minimum 16 MB memory equipped with a CD ROM super VGA display minimum resolution 800 x 600 pixels and 256 colours Input Output connections Connection to PC via serial RS 232 interface by standard 9 pin cable zero modem cable The printer is connected to the parallel port of the PC Counting modes Top fluorescence 340 650 nm Bottom fluorescence Scanning Fast or slow kinetic measurements 22
3. be specified and the time between repeats up to 600 s These fast kinetic measurements can only be made if well mode is selected Slow kinetic measurements can also be made using plate repeats Scan measurements Measurements can be made at different points of a well for samples in which the light output is not homogeneous e g in cell culture wells The number of spots to be measured can be defined Up to ten spots can be measured in both the horizontal and vertical directions maximum of 100 points altogether The distance between spots can be set The distribution of the measurement spots can be square or circular 3 Installation Instructions Unpacking Open the box and remove the packing Lift the instrument up and move it to its place of operation Check the fluorometer for possible transport damage Remove the transport protection piece as follows Pull on the red tab sticking out from the plate loading position 10 The plate holder will come out revealing the transport protection piece Undo the two black screws holding the transport protection piece in place 11 Remove the transport protection piece Remove the protection piece from the filter 1 opening The instrument is now ready to be used 12 4 Instrument Installation Environment Although normal clean laboratory conditions are usually satisfactory as an operational environment it is useful to take the following points into consider
4. screw it back in place Replace the cover then switch on the power Compacting the database You should run the Database Maintenance program on a regular basis weekly or monthly depending how much you work with the fluorometer This program will compact your database and will in some situations increase the software performance If you ignore that the access time will increase You can find the program by clicking on the icon in your Window Start menu It is called Database Maintenance 20 8 Specifications Power requirements Power consumption 180 VA Nominal voltage 100 240 V multirange Nominal frequency 50 60 Hz Input voltage range 85 264 V 47 63 Hz Safety standards 1 EN61010 1 1993 or IEC 1010 1 1990 Al modified Safety requirements for equipment for measurement control and laboratory use 2 EMC Directive immunity and emission EN 61326 1 1997 A1 19998 EN61000 3 2 1995 A1 1998 EN61000 3 3 1995 Environmental conditions Temperature 15 35 C Relative humidity 10 85 Excitation Tungsten halogen lamp 75W lifetime typically gt 300h Spectral range 340 700 nm Filter slides 1 Excitation filter slide with 5 filter positions Comes with high quality interference filters for excitation centre wavelength bandwidth 355 30 nm and 485 15 nm 2 Emission filter slide provided with 4 filter positions with the following filters centre wavelength bandwidth 460 30 and 535
5. 4V 40W Horn Id No 34846 Halogen Photo Optic Lamp 12V 75W Typ 64613 Tungsten Id No 34051 2 Functional description Fluorescence Top Counting Excitation Filter Slide Emission Filter Slide IR Filter Inside the fluorometer there is a tungsten halogen continuous wave lamp 75 W This is used as the light source for fluorescence measurements The lamp unit is thermally isolated from the measuring head by using a light guide to channel the light to the sample The actual wavelength used is selected by means of a slide containing up to 5 filters The filter to be used can be selected by means of the software The spectral range of the Excitation module is 340 700 nm Two positions of the standard 5 position excitation filter slide are used for filters of 355 and 485 nm which are specially designed for fluorescence excitation All standard filters are high quality interference filters with a diameter of 15 mm for Excitation filters and 25 mm for Emission filters There are three empty filter positions into which customized filters can be inserted See the figure Light of the selected wavelength having passed through the light guide is collimated through the sample The stabilised excitation energy is user adjustable to help find the optimum linear range for the samples being measured and thus keep the output signal within the linear range of the instrument Fluorescence is collimated from the plate through a mirror sl
6. Drive gt Settings gt Select Auto insert notification gt OK gt OK gt Yes for rebooting the PC for the change to take effect The following pop up menus will appear when the setup is started Read the menus carefully and follow the instructions for first time installations the default values should be used Setup Fluorometer Setup is preparing the InstallShield R Wizard which will quide you through the rest of the setup process Please wait Welcome Welcome to the Fluorometer Setup program This program will install Fluorometer on your computer It is strongly recommended that you exit all Windows programs before running this Setup program Click Cancel to quit Setup and then close any programs you have running Click Next to continue with the Setup program WARNING This program is protected by copyright law and international treaties Unauthorized reproduction or distribution of this program or any portion of it may result in severe civil and criminal penalties and will be prosecuted to the maximum extent possible under law Click on Next 17 Select setup type Select type of installation by clicking one of the buttons Custom Typical is the option to choose in a normal installation Custom lets you choose which components to install It is recommended for advanced users only lt Back Cancel For first time installation click on Next Note When updating the softwar
7. Instrument Manual Twinkle LB 970T Fluorometer Valid for instruments with software version 2 xx Cestr thie im sn a E ar GIES BERTHOLD TECHNOLOGIES GmbH amp Co KG Calmbacher Str 22 D 75323 Bad Wildbad Germany www BertholdTech com Contents Safety Information and Warnings Safety Instructions for Service Special Spare Parts Functional Description Fluorescence Top Counting Fluorescence Bottom Counting Installation Instructions Instrument Installation Environment Electric Power Connection to other units Information about User Instructions Instrument and User Manual On line Help Routine maintenance Software Installation Minimum System Requirements Printer Installation Installation of the Workstation software on a portable computer Setup Routine Maintenance Cleaning the instrument Changing the CW Lamp Compacting the database Specifications 10 11 13 13 14 15 15 15 15 15 15 15 15 16 19 19 19 19 20 1 Safety Information Warnings and Special Spare Parts Caution This symbol alerts you to important operating considerations or a potential operating condition that could damage equipment Refer to the User s Manual or Instrument s Manual for precautionary instructions General e This equipment must be installed and used in accordance with the manufacturer s recommendations Installation must be performed by properly traine
8. ation The temperature should be in a range of 25 C to 35 C the relative humidity should not be excessive and direct sunlight should not be able to reach the instrument Electric Power Three electrical outlets each with a protective earth should be available with if possible a separate power line for the instrument itself having an isolation switch and a fuse box If excessive fluctuations in the mains voltage are anticipated a main stabilizer may be necessary The main switch appliance coupler and the main fuse is on the backside of the instrument Important Please read the Safety Information and Warnings before you start the instrument Put the instrument in such a position that you will always have a good access to the main switch and the appliance coupler Connection to other units 13 This fluorometer is a compact bench top unit designed for measuring prompt fluorescence by top or bottom counting The software is a 32 bit application running under Windows 95 Windows 98 Windows NT 4 0 or Windows 2000 on a PC connected to the instrument Results are saved in a file format for further connection to a laboratory information system Plates are loaded manually Read under point 7 Routine Maintenance Compacting the database The figure below shows the fluorometer with a PC and a printer Connection Connect the fluorometer to the serial port of the PC using the zero modem cable provided Use the power cable to conne
9. ct the fluorometer to the mains Switch on the PC and the fluorometer 5 Information about User Instructions 14 Instrument and User Manual There are two manuals that come with the fluorometer This Instrument Manual is for the hardware installations and the User Manual for more detailed information necessary for operating the fluorometer The Instrument Manual is normally not needed by the normal user only by the engineer who installs the fluorometer However you might need it if you have to reinstall the system in another location in the future On line Help This is supplied with the software and can be accessed by clicking Help in any window This Help gives detailed information about all features of the operation which concern the normal user service information is not provided There is a table of contents giving an overview of the Help including a How to section a topic index in alphabetical order as well as a search facility enabling any word in the help to be located Topics are connected where relevant by hypertext links so that you can easily find all information on a particular subject Routine maintenance This is maintenance intended to be performed by the user and is described in a separate chapter of this instrument manual Any other maintenance than what is described there should be performed by a qualified service person 6 Software Installation Minimum System Requirements Windows 95 Win
10. d and authorised personnel e This fluorometer is equipped with a three wire grounded plug a plug with a third grounding pin e This a safety feature If your outlet does not accomodate the three wire plug have an electrician install the correct outlet or use an adapter to ground the appliance safely Do not defeat the safety purpose of the grounded plug e These instructions contain important operating and safety information The user must carefully read and understand these instructions before using the instrument e Failure to follow these instructions may invalidate your warranty and or the safe functioning of your equipment e The instrument has been designed to optimise function reliability safety and ease of use It is the user s responsibility to install the instrument in conformance with the local electrical codes e To disconnect the instrument from power the appliance coupler must be removed from the mains e Put the instrument in such a position that it is easy to switch on off e Remove the transport protection before switching on the instrument e Don t block the ventilator while the instrument is running e The LB970T may be transported only in its proper transportation package e For cleaning of the instrument read point 7 Routine Maintenance in this manual e Additionally read the notes in the User Manual on page 20 28 and 56 e Since chemical substances are being used when operating the instrume
11. dows 98 Windows NT 4 0 Windows 2000 Intel Pentium Processor 16 MB RAM or more 30 MB free hard disk space or more CD ROM Drive Super VGA Drive minimum setting is 800 by 600 pixels Printer Installation Install the printer according to the instructions accompanying the printer 15 Installation of the Workstation software on a portable computer General For installing the Workstation on a portable computer connect the instrument to the free COM port of your computer using the standard zero modem cable Windows NT When installing and running the software under Windows NT Windows 2000 the user MUST belong to the local Administrators group This because the security system of Windows NT otherwise prevents the correct installation or operation of the software To add a user to the local Administrators group do the following Note This must be done by a user already belonging to the local Administrators group Start the User Manager program found under Administrative Tools Double click on the username to add to the local Adminstrators group Click the Groups button Add the Administrators group to the Member of listing Click OK Click OK in the User Properties window Exit the User Manager program Proceed as in Windows 95 onauRrwun Windows 95 Workstation Installation to a PC connected to the Net If the installation is made with a certain user logged into the PC a problem occurs when another
12. e acitvate the database for update via custom settings Select startup mode Select startup mode by clicking one of the buttons Select Normal if you want to work with the instrument Select Demo only if your computer is not connected to instrument You can later change this setting from workstation software Click Next with no instrument the workstation can be run in demo mode and the software itself simulates the instrument 18 Start Copying Fluorometer Files If all settings are OK installation is finished 19 7 Routine Maintenance Cleaning the instrument The plate carrier surface should be kept clean to avoid dust and dirt entering the optics at the measuring position The plate carrier should be cleaned at least once a week using a soft cloth or tissue paper soaked in a mild detergent solution or alcohol Changing the CW Lamp If the CW Lamp needs replacing switch off the power and pull off the cover on the right side of the fluorometer to get access to the lamp Unscrew the panel holding the lamp and withdraw it through the hole in the body of the fluorometer Attention The metal panel over the lamp and the lamp itself can be hot Make sure the lamp is switched off and give the lamp a chance to cool before removing it Do not touch the lamp since you might damage it Remove the lamp from its mounting and insert a new one Put the lamp and its mounting back into the instrument and
13. ide a lens system and an 4 position emission filter slide to a low noise photomultiplier spectral range 400 700 nm equipped with fast single photon counting electronics In the emission filter slide two positions are used for emission filters of conventional fluorescence at wavelengths of 460 and 535 nm Note when the properties of a label are defined then the filters to be used for measurements with that label are specified When a protocol is selected for a measurement the appropriate filters are moved into position automatically Both excitation and emission light are directed and collected from the top of the well See the next section for information on bottom counting Fluorescence bottom counting Excitation Filter Slide Emission Filter Slide Aperture Z AZ Yyz In the case of bottom counting of prompt fluororescence samples excitation light is directed through a light guide underneath the plate Emission light goes back along the same light guide to the optical block and passes through the emission filter to the PMT Bottom counting means that measurements can be made for samples in which cells are at the bottom of the wells or there are lids on wells Switching between top and bottom counting is done by software and both methods can be used for the same plate Kinetic measurements To investigate the kinetics of a reaction repeat measurements are necessary The number of repeats up to 100 can
14. nt the manufacturer s warning and safety precautions R and S sets have to be observed To avoid personal injury e The interior of the instrument can reach temperatures that can cause burns Avoid contact with the heating plates Some parts of the instrument can remain hot without visual indication for some time after the power is turned off e The metal part over the lamp can be hot Don t start the instrument without the plastic cover over the lamp e Don t use a flammable liquid inside the instrument A fire hazard may result e Don t open the flap while the instrument is running Safety instruction for service e Disconnect the instrument from the main power prior to maintenance and servicing e When the top part of the housing is removed no safety measures are in effect Be careful if the instrument is running e When the top part of the housing is removed take care with any moving parts e High voltage is generated in the multiplier unit Don t touch the unit while the instrument is running e Proper operation is ensured only when using original spare parts Special Spare Parts The following spare parts are safety parts Use always the original part from the manufacturer or direct agent Fuse 2 00A T 5x20 UL 1986 CSA C22 2 Id No 09892 Power Supply JWS 150 24 A 100 240VAC 24V 6 5A Lambda Id No 34846 Power Entry Modul w Switch KD14 1101 151 Schurter Id No 34036 Heating Plate 2
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