User`s Guide Standard Dilutor v1.02
Contents
1. Error or malfunctions may be caused by wrong parameter values Restoring the factory settings recovers the standard value for every parameter and lets the instrument in a known must run state Beware that the printer settings are also reinitiated After restoring factory settings when a printer is used the communication parameters must be reset To restore factory settings e Turn the instrument off e Turn on again and when the splash screen appears keep the upper left zone pressed until the default parameters dialog appears e The default parameters dialog screen is displayed Clear All Procedures HO Using the up and down arrows select the desired value Yes No Press the shrimp symbol to return to main menu Changing the Peristaltic Pump Head To change the peristaltic pump head do not need open the instrument Look at the next drawing to take it out and replace it for the new one following the steps in the opposite way CAT No 10101 OS yy CAT No 10100 N ly A O Pa C go y CAT No 10102 Contact the Technical Service in your country or IUL headquarters if any problem persists IUL S A Torrent de l Estadella 22 08030 BARCELONA SPAIN Phone 34 932740232 Fax 34 932740144 hitp www lul inst com E Mail iul iul inst com 9 s ACCESSORIES AND RELATED INSTRUMENTS OPTIONAL ACCESSORIES As follows you can see the list of the optional accessories with their cat2. Paz ni Gr X345664A 3 0 WU 1 In the top region starting from the left there is a symbol showing a bag This sign is flashing when the unit waits for a new bag Next to if there is a push button with a symbol 0 0 to perform the tare Also the instrument asks for a tare action when if is flashing When the stability function has been set to a value other than the default O a shaking weight icon is shown meaning that the instrument is tolerant to vibrations In the right hand side there is the current weight display The grams g character flashes when the welght measurement is not stable 2 In the middle region there is the information related with the sample symbolically represented as a shrimp The left sample symbol is flashing when the unit waits for the sample There is the SAMPLE ID next to it This can be introduced manually or using the Bar Code Reader device connected to the unit In this case alphanumeric information can be accepted In the middle there is the sample weight took at the moment that the arm was turned down there is also the dilution factor used The operator name or number is shown on the right 3 The arm is represented by a flashing icon it indicates if the arm is backward or forwards The pump number as well as the DILUENT ID used also alphanumeric information these are displayed at its right side There is a push button at the right side to open the ufilities dialog
3. 4 s INSTALLING PERIPHERALS The main unit can accept three different kinds of peripherals e Serial Printer e UL Barcode Reader See Chapter 9 e UL 4 pumps accessory See Chapter 9 The last two items do not need any special configuration The main unit recognizes them and work directly once connected Connecting the printer The Smart Dilutor has a standard SUB D 9 pin RS 232 female connector that can be connected to an external printer provided with RS 232 serial interface Look at the chapter 9 for an optional printer cable Configuring the Communication Parameters The serial interface specification on the printer and the Smart Dilutor must have the same values Look at the printer users manual to know what the values of its communication settings are see ESTABLISHING NEW SETTINGS at the chapter 6 to configure the Smart Dilutor Instance of a results ticket IUD Smart Dior 2 5 02 01 2010 120000 Operator Id 002700S402 Sample Id 6 Sample Weight 0009 89 g DEIS Lacio 037010 Dal Los 0000000910 BE I Total Weight 00099 0 q Final accuracy 100 103 Cal brat on 30 12 2009 O DILUTING AND DISPENSING PERFORMING A DILUTION PROCESS PUTTING THE BAG IN THE HOLDER The bag holder is flexible and in its upper part there are two strips of adhesive putty Place the mouth of the bag at the same level of the upper edge of the walls of the holder and with the other hand move the sides of the holder closer
4. generates to the ADC and from that one to the microprocessor which uninterruptedly monitors the weight in the pan and takes the necessary decisions in order to activate the diluent pouring pump proportionally Once the programmed dilution has been reached the system notifies the operator that the process has finished The software also takes into account the use of the instrument as a dispensing device for a previously determined quantity of liquid using any kind of container N B TO OBTAIN A GREAT PERFORMANCE OF THE INSTRUMENT IT WILL NEED TO BE KEPT CLEAN BY THE USER See Chapter 8 2 TECHNICAL SPECIFICATIONS GENERAL FEATURES e Dimensions LxWxH e Weight e Data output input OPERATING CONDITIONS e Mains e Power e Working temperature WEIGHING SPECIFICATIONS e Weighing range e Sample weight resolution e Weight accuracy e Calibration weight DILUTION SPECIFICATIONS e Dilution ratio e Minimum sample weight e Maximum sample weight e Dilution accuracy e Delivery speed DISPENSING SPECIFICATIONS e Dispensing range e Dispensing accuracy e Delivery speed REMARKS 300x325x365 mm 6 9 Kg serial RS232 IUL Barcode see Chapter 9 100 240 ACV 50 60 Hz 40 w 5 C to 40 C 2400 9 tare included 0 01 g 1 for weight 10g 2 for weight gt 59 100 to 2000 g user configurable 100 g steps any fraction with numerator 1 to 99 and denominator 1 to 999 user configurable
5. pincers that holds them are made of stainless steel The Masticator is the instrument that homogenizes the sample with the solvent after the dilution is made by the Smart Dilutor The Bio Dilutor is an instrument with a microprocessor that carries the serial dilution process of the sample needs before analysis 1 0 WARRANTY CONDITIONS LIMITED WARRANTY IUL S A warrants the Instrument disposables and replacement items to the original Purchaser for a period of one 1 year after date of receipt by the end user against defects in material and workmanship IUL S A agrees to correct either by repair or at its election by replacement any such defect found on examination to have occurred under normal use and service during such one 1 year period provided IUL is prompily notified in writing upon discovery of such defect to the adaress of one of its workshops noted in the User s Guide Upon discovery of such defect the instrument should be sent to an IUL workshop noted in this User s Guide postage prepaid EXCLUSIONS IUL S A shall not be liable as to any defect arising from abuse of the Instrument failure to operate and maintain the Instrument in accordance with the Users Guide supplied with the Instrument repair service alteration or modification of the Instrument by any person other than the authorized service personnel of IUL modification change or reuse of the disposables supplied by IUL for use in the Instrument
6. the pump to be used If necessary set the solvent ID if can be entered using barcode reader or the alohanumeric keyboard displayed at the time that this value is selected Note This will be the procedure in the rest of the editable values If the Dilution process is accepted the screen remains as if was The Dilution factor can be changed by editing the second row The limits of the sample weight can be fixed on the third row The fourth row set the way to enter the sample ID by default this ID is a number that will be increased automatically in every operation if the Bar Code Reader is installed optional accessory when an operation starts it lights and the Bar Code read is stored as the sample ID By default the keyboard icon is tagged but if you push its near right button the tag is removed this means that in every new operation this keyboard is showed fm GOO The Sample ID can be entered using this keyboard once entered push the shrimp flask button to begin the operation If the Bar Code reader is installed it lights and the read Bar Code is stored as a Sample ID the instrument will begin the operation automatically E Ifinstep C Dispensing was selected the screen displayed is the following El Select the pump to be used E2 If necessary set the solvent ID E3 Set the weight to be dispensed F To access to other menus such as the Utilities Calibration and User ID use the icons of the last row If the i
7. until they are squeezing the mouth of the bag Letting go the holder the bag will remain opened ready to be filled Press 0 0 Tare to cancel out the bag weight During tare process is displayed on the weight field DILUTING A SAMPLE To get a sample diluted to a fixed ratio e select the pump to be used The instrument has been designed in such a way that the pump has not only the desired solvent or diluent assigned but also the type of work to perform dilution or dispensing as well as the parameters related dilution factor and total weight e Introduce the sample carefully in the bag e Pull the arm tube forward carefully It will prevent a sample weighing error caused by a hit on the instrument when the weight reading is transferred to the memory The pump starts delivering the solvent while the screen shows the current weight continuously changing to reach the final target weight As the weight approaches the end value the speed of the pump is slowed down to reach if in a good approach When the target weight is attained the system produces a short beep and if programmed to do so prints a ticket Moving the tube backward the instrument is ready for the next dilution PERFORMING A DISPENSING DISPENSING A FIXED WEIGHT OF DILUENT To dispense a fixed weight of diluent e select the pump that has the dispensing program with the appropriate weight e Place the container where the diluent must be transferred e P
8. HE PORT RS 232 Once enabled the parameters configurable from Smart Dilutor of the RS 232 port and his values formed by defect are the following Parameter Value Data bits Stop bits 1 bit Flow Cril RTS CTS MODIFY THE PARAMETERS OF THE PORT RS 232 Push the spanner symbol from any screen where if appears to get access to the screen of the configurable parameters A Push the up down arrows to select the parameters of the RS 232 port and push button y B Using the up down arrows all parameter will be showed C Push W to see the value of the parameter selected to change that use the up down arrows Press y to confirm the desired value D Press the shrimp symbol to back to the main menu RS 232 9 Pin Female Socket Pinout 1 NotConnected _______ DSR PAI YA IS SRK CTS 4 DIR Intemally jumpered with DSR 9 Not Connected 7 s e Y 8 MAINTENANCE AND SERVICE CLEANING THE INSTRUMENT Cleaning the screen and other surfaces with a brush hot water and soap Cleaning the pumps Use a brush with hot water and soap to clean the pump body Then place a cloth soaked with water and soap instead of the tube and run an operation to move the rollers DISINFECTION OF THE INSTRUMENT Clean all the surfaces of the device except bag holder with a wet cloth with deionized water and 70 ethanol or isopropyl alcohol For the bag holder you should use water with 3 bleach Restoring Factory Settings
9. SMART DILUTOR W Firmware Versions 2 2X USER S GUIDE Distribu par Z A de Gesvrine 4 rue K pler B P 4125 44241 La Chapelle sur Erdre Cedex France t 33 0 2 40 93 53 53 f 33 0 2 40 93 41 00 commercial humeau com Laboratoires Humeau SMART DILUTOR W Firmware Versions 2 2x USER S GUIDE MANUAL Copyright 2012 2014 IUL S A All rights reserved Cat No 50007662 Doc No 50007662 04 INDEX E INTRODUCTION SR nn OA 1 WHAT IS THE SMART DILUTOR WEINE ie u 1 2 TECHNICAL SPECIFICATIONS sn ie 2 95 INSTALUNG AND STARTING UP co it 3 4 INSTALUNO PERIPHERALS ss weet 6 9 DILUTING AND DISPENSING coi an dents 7 PUTUNG THE BAGINMEIE ROEDER ze ea 7 DILUTING A SAMPLE rs een A AA A a AN 7 DISPENSING A FXEDWEIGA TOF DILJEN T see ee aaa 8 O UIEMESAND CALIBRATION AAA 9 ESIABESEINGANEWBROGRAN ee ete eine 9 EPA 1AE OPERATOR Do een a ra 12 ESTABLISHING NEW SE TIN Sida ee 12 ESIABLBEINSANEWSTBLIFFUNGTON 2 to 13 WARNING taras atado odas 13 Li COMMUNICATIONS Sia 14 VALUES CONFIGURABLES OF THE PORTRS 29 2e sen aa 14 MODIEFTRAEPARAMETERS OPTAEPORTER gt S 2 3121 14 O MAINTENANCE AND SERVICE ne ne am AAA AA 15 9 ACCESSORIES AND RELATED INSTRUMENTS cricoid aieo no ieia EVEN OEA EN TE EAN 17 OPHONALACCESSORIES Heels 17 RELATED INSTRUMENTS Si ae a a 17 10 WARRANTY CONDITIONS nn 18 FRONT VIEW 1 Keyboard amp Display gt 2 Pumps lor 2 DN 3 Arm for pouring tube AS SS O
10. alogue number CAT No DESCRIPTION 90002528 set of tubing 100 cm longer to bottle for use with 90007528 90008181 or 90008332 90002530 Silicone tube 5 650 m for use with 90007528 90008181 or 90008332 90002738 Holder for media bags 90002800 Dilution arm screw 90002806 Multilevel dilution dispensing arm 90002836 support bar code reader 90002837 Bar Code Reader with holder for Dilution Arm 90002838 Weighing platform for beakers flasks 90002853 Tubing set with silicone lid for GL 45 long 90002854 Tubing for Metallic edge for use with 90007528 90008181 or 90008332 90002855 Tubing for metallic edge long 130cm for use with 90007528 90008181or 90008332 90002885 Accesory for 80 ml Bags 90005510 Communications Cable RS 232 with Adapters PC Printer 1 8m 9000570 Bar Code Reader MMJ7 90007528 Edge for Septum bags small 90008165 Autoclavable bottle 5 I PIREX GL 45 90008181 silicone lid with inox tubes for GL 45 90008182 set of 2 autoclavable 0 2 u filters for bottle stopper 90008183 Envelope with sticky putty Blu Tack 90008331 stainless steel edge of dispensing tube 90008332 Edge For Septum Bags big 90008390 Plastic screw for pan with O ring for use with 90001 1103 900011102 Adjustable two pumps arm 900011103 Platform for Smart RELATED INSTRUMENTS The Porta bag is a device that allows holding six bags filled with the sample and the diluent in a vertical position without the worrying of spilling its contents The tray and the
11. default 1 9 compatible with ratio tare and weight range 1 for dilution weight 100 g 2 for dilution weight 50 9 950 mi min 20 g to full weighing range 1 for dispensed weight 100 g 2 for dispensed weight gt 50 g 950 mi min l Specifications above are given for operation with standard tubing bore 5 mm stability function set to O default the instrument lying on a stable surface free of vibrations and in absence of air movement or disturbances 2 Accuracy specifications comply with the requirements of ISO 7218 2007 Ama 1 2013 and ISO 6887 1 1999 3 s INSTALLING AND STARTING UP When you receive the instrument make sure that the package contains The apparatus itself with the mounted pump head one or two according model The rigid arm of the pouring tube The bag holder The 5 liters bottle Two 0 2 micron filters for the bottle stopper spare adhesive to hold up the bags Intake pouring and peristaltic pump tube with stopper WARNING ONCE INSTALLED DO NOT TURN PUSH OR PULL THE BAG HOLDER THE LOAD CELL CAN BE DAMAGED Likewise make sure that none of the parts has suffered any apparent damage A Place the instrument upon a rigid vibration free support remembering that the Smart dilutor contains a weighing cell and must be located and maintained as though it were a balance B Place the bag holder into its position on the weighing disc in the desired alignment and screw it It mu
12. ed to Purchaser by IUL shall be treated by Purchaser as confidential and proprietary and protected by copyright and shall not be disclosed to third parties Purchaser shall not reproduce copy or substantially duplicate any parts of said materials without the written consent of IUL In the event any Instrument is returned to IUL for any reason or transferred to a third party Purchaser shall return all of said materials including copies thereof to IUL Acceptance by IUL of purchaser s order is expressly limited to and conditioned upon purchaser s acceptance of and assent to these terms and conditions referred to herein Any additional inconsistent or different terms and conditions contained in purchasers purchase order or other documents submited by purchaser to IUL at any time are hereby expressly rejected by IUL Purchaser acknowledges that notwithstanding anything to the contrary contained in its purchase order or other documents receipt and acceptance by purchaser of the Instrument shall constitute acceptance by purchaser of these terms and conditions Distribu par Z A de Gesvrine 4 rue K pler B P 4125 44241 La Chapelle sur Erdre Cedex France t 33 0 2 40 93 53 53 f 33 0 2 40 93 41 00 commercial humeau com Laboratoires Humeau
13. lues displayed can be changed by using the arrows and the Y button D Change manually the operator ID or using the IUL Barcode reader if connected ESTABLISHING NEW SETTINGS A Push the spanner symbol B Anew screen with four rows appears EB sus Using the arrows and the ok symbol all the paris of the screen can be changed C Change the time to the correct value Change the date to the correct value D E RS 232 options F Pushing with the up and down arrows Make the buzzer enable or disable ESTABLISHING A NEW STABILITY FUNCTION When an instrument is placed on a surface suffering mechanical vibrations an important delay to reach the end point of operation may occur The tolerance to vibrations can be increased through the modification of the stability function value A Push the shaking weight icon B Using the arrows select the stability that you want to use O is the default value for no vibration surfaces 4 is the highest value for strong vibrations 20 WARNING Values other than O can worsen device precission WARNING SELECTING HIGHER VALUES IN STABLE CONDITIONS MAY REDUCE THE DILUTION TIME BUT THE ACCURACY WILL BE WORSENED COMMUNICATIONS The Smart Dilutor communications with other instruments or computers can be done by its RS 232 input output port and a socket for the IUL Bar Code Reader Cat 90005701 If you want to use it request it to IUL S A VALUES CONFIGURABLES OF T
14. ne pump 9002821 Two pumps 9002822 4 Bag holder 900099639 5 Knurled screw 90007582 REAR VIEW 6 Screw for arm Knurled nut for two pumps arm 90002796 Knurled screw one amp two pumps 90002800 Main switch RS 232 DB9 Socket IUL Barcode Mains cable Tube clamp Pa 5 12 LE To al PUMP HEAD N a 12 Head IT IA LA 13 Door ka SS VAN 14 Rotor PMA 15 Tube Standard 90002825 37 1 INTRODUCTION The repeatability of results in microbiology depends to a great extent upon the sample preparation These days the use of sterile bags to carry out homogenization processes has become essential It is Undeniable that as well as its convenience it brings a greater degree of safety and hygiene than any other kind On the other hand the manipulation of homogenizer bags when they have no consistency is an awkward operation especially if in order to obtain repeated results the sample must be diluted with a proportional amount of diluent liquid WHAT IS THE SMART DILUTOR WHITE The Smart Dilutor White is a diluter especially designed for the carrying out of the dilution of microbiological samples directly in the sterile homogenizer bags Because of the fact that the samples are usually solid semi solid paste etc the gravimetric type of dilution has been chosen and has been incorporated into a weighing cell that is the heart of the system This cell is continuously sending the signal that it
15. nstrument has two or more pumps installed these can be remote a chain icon is displayed if allows to work the pumps together chain bond or separately chain open these icons are displayed in both the disoensing and the dilution screens PERFORM A NEW CALIBRATION WARNING CALIBRATION MUST BE PERFORMED ON A STABLE SURFACE FREE OF VIBRATIONS AND WITH THE STABILITY FUNCTION SET TO 0 DEFAULT A Push the weight icon B Anew screen is displayed C If the bag holder is empty the zero push button is flashing push It D Now the calibration weight icon is flashing Put the calibration weight on the center of the platform the calibration weight can be changed by using the arrows The current measured weight is displayed below the calibration weight E Push the weight button If the weight is wrong the instrument cannot be calibrated Instance of a calibration ticket IUL Smart Dilutor 2 xx 30 12 2009 17 59 59 Operator Id 0027005402 Cal Weight 1000 00 g Zero Offset 1093835 1 Span Gain 678 2 1 Zero Offset is the O gr corresponding value in internal units It has utility for technical service only 2 Span gain is the ratio between the calibration weight in ugr and the distance from its corresponding value in internal units to Zero Offsef It has utility for technical service only REPLACE THE OPERATOR ID A Push the operator symbol B Anew screen appears C Al the va
16. ress 0 0 Tare to cancel out the container weight e Pull the arm tube forward The pump starts to deliver solvent while the screen shows the current weight continuously changing to reach the final target weight PAUSING THE PROCESS There are two ways to pause a running process e Pushing the arm tube backward Pulling the arm tube forward again will continue the addition to the target weight WARNING PUSH THE ARM TUBE BACKWARD SLIGHTLY 1 E WITHOUT TAKING OUT THE POURING NOZZLE FROM THE BAG ONCE THE MOTOR STOPPED PUSH THE ARM TUBE BACKWARD COMPLETELY THIS ACTION AVOIDS SPILL OUT OF THE BAG e Pushing the pause button pushing it again the dispensing will continue za 34567 010 009 E LL gie ce UTILITIES AND CALIBRATION The utilities main screen allows establishing the following e ANEW PROGRAM THE PUMP ICON e ANEW CALIBRATION THE WEIGHT ICON e ANEW OPERATOR THE PERSON ICON e NEW SETTINGS SPANNER ICON e STABILITY FUNCTION SHAKING WEIGHT ICON ESTABLISHING A NEW PROGRAM A Push the pump icon B Anew screen with these icons is displayed 5 C Push the shrimp icon to program a dilution Push the flask icon to program a dispensing D The following screen is displayed if the shrimp icon has been pushed otherwise go to the section E DI D2 D3 DA DS D6 D7 D8 All the values displayed can be changed by using the arows and the Y button Select
17. s operation outside of the environmental specifications for the product listed in the User s Guide or accidental damage to the Instrument NO OTHER WARRANTIES The warranty set forth above is exclusive and no other warranty whether written or oral is expressed or implied IUL and its suppliers specifically disclaim any implied warranties including merchantability and fitness for a particular purpose NO LIABILITY FOR CONSEQUENTIAL DAMAGES IUL or its suppliers shall not be liable as to any damage whatsoever including without limitation special incidental consequential or indirect damages for personal injury loss of business profits business interruption loss of business information or any other pecuniary loss or defect arising from abuse of the Instruments failure to operate and maintain the Instruments in accordance with the Users Guide repair service alteration or modification of the Instruments by any person other than authorized service personnel of IUL or modification change or reuse of the disposables supplied by IUL for use in the Instruments Important information Purchaser shall indemnify IUL and hold it harmless for all expenses damages attorneys fees and costs resulting from claimed infringement from patents trademarks or industrial property rights arising out of compliance by IUL with Purchaser s specifications designs or instructions All software firmware hardware and manuals relating to Instruments suppli
18. st be done without using any tool WARNING WITHOUT THE BAG HOLDER OR ANY OPTIONAL PLATFORM THE INSTRUMENT WILL NOT BE USEFUL THE WEIGHT READINGS MAY BE WRONG C Screw the rigid arm no 3 in the right hand side of the unit facing you and fix it with the screw no 6 Check the front back movement of the arm D To install the tube no 15 into the head of peristaltic pump no 12 make the door open no 13 the rotor no 14 is now accessible E Place the tube clamp in its housing F Install the tube below the rotor G Place the other clamp in its housing WARNING TAKE SPECIAL CARE NOT TO GIVE THE TUBE A 180 TURN OVER ITSELF CREATING A BLOCKAGE THAT WILL PREVENT THE LIQUID FROM COMING OUT H Check that the tube is centered I Close the door J Place the pouring nozzle into its housing on the rigid arm 3 Its shape allows directing the flow against the wall of the bag to prevent splash K Install a filter into the free tube of the stopper it cleans the air which is introduced into the bottle when if empties L Connect the power cord and with the rigid arm in the backward position turn on the main switch M The instrument shows its firmware version in the screen while performing a fast auto test which leaves it in the main screen smari dilutor iy IUL In the main screen the instrument is idle and ready to go The screen is divided in three horizontal regions o ya 3 567 000 009
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