Z233M - Phenix Research Products
Contents
1. INSTRUCTION MANUAL MICROCENTRIFUGE Z 233 M2 National Labnet Company PO Box 841 Woodbridge NJ 07095 Phone 732 417 0700 Fax 732 417 1750 INDEX L General Information Deal 2 3 4 1 5 6 7 2 1 2 2 2 3 Precaution and hazards Description Do not operate the centrifuge uncer the following conditions Safety standards Technical data Accessories supolied with each centrifuge mit Warranty Installation Unpacking the centrifuge Required space How to install and load a rotor 3 1 3 2 3 3 3 4 Mounting and securing an angle rotor Set or change rotor code Overloading rotors Removing the rotor Operation 4 4 4 4 o uoun WwW 4 Preselection of speed RCF Preselection of operating time Preselection of brake intensity and acceleration Keyboard Starting the centrifuge quick key Temperture 5 1 Temperture Safety facilities 6 Imbalance Service and Maintenance 6 1 6 2 6 3 6 4 TL 7 2 Service and inspection of centrifuge Cleaning the centrifuge Cleaning of centrifuce after breakage of glass tubes bottles Disinfection Breakdown Emergency lid release Check list Trouble shooting General Information 1 1 Precautions and hazards Before putting the centrifuge into operation please read this instruction manual carefully The centrifuge must not be operat2. alkaline amino strong acids solutions containg heavy metals waterfree and chlorinated solvents S salt solutions e g seawater Cleaning The purpose of a thorough cleaning is beside hygienic reasons the avoidance of corrosion by soiling In orcer to avoid the damage of anodized parts such as rotors reduction plates ect only neutral cleaning agents with a ph value 6 8 should be used Never use an alkaline cleaning agents ph gt 8 After cleaning please ensure that all parts are dried thoroughly by hand or in a wamrair cabinet max temperature 50 C It is recommended that all anodized aluminum parts are regularly treated with corrosion oil so that their durability will be increased and the corrosiom reduced Usually when used in a cold room humidity and non hermetically closed samples may cause condensation to form The condensation should be removed regulary with a cloth from the rotor har Unplug the centrifuge when it is not being used allow the chamber to reach roan temperature and dry out the chamber with a cloth Cleaning of centrifuge after breakage of glass tubes glass bottles With high g values there is a possibility that tube breakace will occur Shoul happen the centrifuge rotor buckets adapters and the rotor chamber must thoroughly cleaned and all broken particles removed inmediately If this is no core they co
3. during the run the speed can be changed as follows Press the preset key 4 hold it and at the same time chance speed or g valu with the speed knob The max speed for all rotors is 14 000 rpm mnagdgoganmnaad HOoo0aqoocanpoeaUL Preselection of operating time You can adjust the desired operating time between 1 and 60 minutes or hold With the centrifuge lid open you can preset the operating time with the knob During a run and with the centrifuge lid closed you have to press the preset key 1 hold it and change cperating time by tuming the knco 3 The preselected running time will be indicated on the time display 2 At the ed of a nn the preset operating time will be k for further nns d oooagamdn na ooaopoaUL HBouunuHUwvaAaDaAg anaoaoononnournuugd oa ou c NDAagooaecaaADg Figure 7 For continuous runs tum the knobo clockwise to the limit stop The continuous run will be indicated on the digital display with two minus signs You can check the operating time during a continuous run as follows Press the start botton 4 After this the qoerating time will be automatically displayed in the following sequence For example 4 hours and 13 minutes operating time min sec min sec min sec min sec gt H gt gt m gt gt continuous run symbol h for ho
4. soon as the rotor has stopped the error message can be reset by opening and closing the trifv lid Error no 50 99 Emergency stop If this occurs the frequency converter will be switched off This means that the rotor will be stopped brakeless The error message can only be reset by unpluging the power cord and then plug back in If the unit stops due to an error you should restart the unit to check if the error oxurs again The error nurbers which are not listed in this chapter are not in use at the tine of publication and they are reserved for future use in widening the error recognition program Figure 11 Error no 1 Imbalance Reason Tnoorrect leading of the rotor Balance your samples Reason Incorrect adjustment of the imbalance switch Action Imbalance switch has to be readjusted call service Error no 2 Permanent imbalance signal Reason Position of the imbalance switch not correct Action Imbalance switch has to be readjusted call service Reason Imbalance switch is defective Action Imbalance switch has to be replaced call service Error no 25 Power failure Reason Power failure while rotor is in motion Action Q n and reclose the lid restart the centrifuge Error no 36 Relay for the frequency converter cannot be released R
5. 230 V 50 Hz 120 V 60 Hz Current 6A 6 5A Connected load 460 Watt 610 Watt Interference suppression DE 0871 interference degr Loe dept at Lalnet 732 417 0700 National Labnet Company 162 Fernwood Avenue Edison NJ 08837 Accessories supplied with each centrifuge unit 2 Fine wire fuses 6 3 AT 1 Instruction manual 1 Tool for removing the rotor Warranty This centrifuge has been subjected to thorough testing and quality control during the production process In the unlikely event of any manufacturing faults occuring the centrifuge and rotors are covered by warranty for a period of one year from cate of celivery This warranty becares invalid in case of improper operation use of non appropriate spare parts or accessories and non authorized modification of rotor or centrifuge The manufacturer reserves the right to make technical modifications to the product for the purpose of technical improvement Unpacking the centrifuge The Z 233 M2 is supplied in a carton with corrugated cardboard inserts Open the carton and remove the corrugated cardboard inserts then gently remove centrifuge from carton The instruction manual and the accessories mentioned under 1 6 should be kept with the centrifuge Please keep all packaging in safe storage for at least 1 year for warranty purposes Required space The centrifuge should be installed on a rigid even surface The centri
6. display for speed rm ref g x 10 the mmber cock for the set rotor type apars On the digital display time min sec the letters ro rotor appears Turn the speed knob 1 see figure 4 till the required rotor coc appears c the display Rotor Rotor code C0230 2A C0200 95 C0230 9A 44 x 1 5m C0230 43A 64 x 05ml C0230 55A 24 x 1 5 24 x 0 5ml C0230 TSA Strip tube 4 x 8 0 2m After doing that disconnect the power supoly and put it back and the new rotor number is stored Overloading rotors The max load permitted for a rotor which is determined by the manufacturer as well as the max speed allowed with the rotor se indicatios cn the rotor itself must not be exceeded The liquids with which the rotors are loaded should have an average homogeneous density of 1 2g per ml or less when the rotor is running at maximm speed To spin liquids of a higher density the speed has to reduced according to the following fomula 1 2 Reduced speed eN ERA ET x max speed N aax value x 4000 3360 rpm In case you have any questions please contact the National Labnet Removing the rotor Take off the rotor lid and hold the rotor with me hand Tum the rotor nut clockwise until the rotor is loose and take the rotor vertically off the shaft ATTENTION Never operate the centrifuge with rotors or buckets which show any signs of corrosion or mechanical damage Never operat
7. e with strongly corrosive materials which could damage rotor and buckets 4 Operation Power You can switch on the centrifuge by plugging in the power supply The digital indications on the display will light up when the power is on Lid release When the green control lamp of the lid key 1 lights the rotor is not moving ard the centrifuge lid is ready to g n Press the Lid key 1 see figure 5 to g n the lid Te green omtrol larp 2 will extinguish as soon as the lid is qpered or the unit started d uouuooong anaaoaoononnournuygd oeroanodredaedda bDoaoeroaagd oooecoaad pDoaneoadga ooagogo0gcaca NDAaAgoocaaDg HERE 9g 22220124 poaoomoascooo tonuan cron TT eee ee ves gooqoeaqga ognin L va Lid lock Close the centrifuge lid after the rotor has been fixed correctly as scribed The centrifuge can only be started when the lid is closed correctly The green control lap of the lid key will light as soon as the lid is closed correctly When the rotor starts accelerating the control lamp of the lid key extinguishes and the lid cannot be opened Preselection of speed RCF When the centrifuge lid is open you can preset the required speed or rcf with the speed knob l With the speed rcf key 2 you can change the display fran speed to g value The green control lames 3 show which m is activated If the centrifuce lid is closed and
8. eason Defect on the power board Action Call servi Reason Centrifuge lid is jaming Action Q n the centrifuge lid manually Grease the lid lock lightly If this failure coours agin call service Error no 50 51 Memory failure Reason Internal or external memory failure Action Restart the unit if the failure coours again call service Error no 55 Overspeed Reason Overspeed sensor or engine speed sensor defective Action Call service Error no 60 Engine speed sensor signal is missing Reason Engine speed sensor cefective or parting of a cable at the sensd Action Call service Error no 82 Cutoff power board frequency converter Reason Overcurrent or undervoltage due to power supply fluctuations Action Restart the unit take care that the power supply is stable Error no 83 Preset speed cannot be reached Reason Preset speed cannot be reached Action Call service Error no 90 Emergency lid release Reason Lid has been geened during centrifuge run Action Close the lid Danger of accident Qly open centrifuge lid when rotor stands still Reason Control switch of lid lock cefective Acticn Call service
9. ed by unqualified persons not familar with the correct use and intended purpose of the machine Please use only the original spare parts For personal and enviromental safety pay special attention to the following precautions The Hermle Z 233 M2 is neither explosion proof nor inert gas shielded and should therefore never be operated in explosive hazardous locations Never stay in the safety zone of 30 cm around the centrifuge or deposit dangerous materials inside this zone during centrifugation The centrifugation of flammable explosive or radioactive samples is not allowed Do not spin samples which can chemically react with each other when exposed to air Never spin toxical or pathological material without adequate safety precautions i e centrifuging of buckets tubes without or with defective hemetic sealing is not allowed The end user should perfom appropriate disinfection procedures in the event of dangerous materials contaminating the centrifuge or its accessories General universal laboratory precautions should be ooserved if infectious materials are centrifuged If necessary please contact a health safety officer Tt is prohibited to nn the centrifuge with rotors not suited for this centrifice m l Uncer no circumstances should the centrifuge lid be opened while the rotor is still turning running with a speed of more than 2 meters per second The foll
10. f The unit is nt installed correctly Tre unit is partly dismantled The unit was serviced by non authorized or non qualified personnel The rotor is not installed securely o the motor shaft Rotors or accessories will be used which are not authorized for this unit by Hermle or Labnet Exception Commercially available tubes or bottles made of glass or plastic The unit is in explosive atmospheres Combustible or explosive samples are used Samples are used which can mix and react chemically with each other Safety standards The centrifuge corresponds to the general requirements set by German law for medical apparatus MedGV group 3 The following standards have been considered for the production of our centrifuges Accident prevention rules for centrifuges UVV VBG 7z Accident prevention rules for electrical equipment amp installations UW VBG 4 International Standard IEC 1010 1 and IEC 1010 2 D European Standard PR EN 61010 1 and PR EN 61010 2 2 Electrical interference suppression according to interference degree as per VDE 0871 Manufacturer Hermle Labortechnik GmbH Type Z 233 M2 Dimensions Width Depth Height Weight Max speed 14 000 rpm Max volume 44 x 2 0mL Max RCF 18 620 x g Admiss density 1 2 kg dm Acmiss kinetic energy 3761 Nm Electrical connection AC
11. ft ensuring that the pins align correctly with the rotor SS Figure la Figure Ib Hold the rotor with one hand and secure the rotor to the shaft by tuming the rotor nut 1 counter clockwise see figure 2 ATTENTION If using a rotor that was not originally supplied with the centrifuge you have to change the standard rotor code which is programmed see chapter 3 2 page 7 E Load the rotor symmetrically by inserting tubes into spaces opposite from each other The rotor must always be balanced in this fashion prior to operation ATTENTION Before operation secure the rotor lid to the rotor by pressing the snap connector lightly on rotor mut see figure 3 3 2 Set or change rotor code With a new unit the centrifuge is programmed with the rotor coce for the rotor that has been ordered with the machine If you wish to use another rotor you have to change the rotor cock Please proceed as follows Qoen centrifuge lid and unplug the centrifuge Press the lid key 2 see figure 4 hold it and at the sare time plug in th centrifig cop oocad 1P zooacoaod E paqcocnng oog0anoacca opnocnonoand goaaadgugndeouy NDAaAgoOoecaaDd xm ep 2233M 2 q poaocaaascoua Bn pono 02ra n 7 a ee p E a T G 1 2 Note To check rotor code turn power on plug in open lid and press the Quick button Rotor code is now displayed Figure 4 Qn the digital
12. fuge should only be operated on a stable laboratory table cabinet etc Balance the centrifuge with a spirit level To guarantee the recessary heat dissipation the unit must be situated so that there is a space of at least 15 cm cm each sic of the unit Never place the centrifuge in an area subject to excessive heat e g strong sunlight as the performance of the unit is based upon an anbient temperatur of 23 C Attention New safety standards require a safety margin space of 30 cm around the centrifuge Mark this area to indicate that personnel and dangerous material e g flammable or infectious liquids should be kept outside this area during operation TnstaLlatim that the power supply corresponds to that on the manufacturer s rating label which is mounted on the rear panel then connect the power cord to the centrifuge and the socket The line voltage circuit breaker has a maxinum of 16 Amp type K slow release for commonly used instruments An emergency switch is installed outside the room to disconnect the power supply in case of a troubled run The digital indications on the display are Lighting up Press the Lid key You can open the centrifuce lid now 3 How to install and load a rotor 3 1 Mounting and securing an angle rotor Clean the motor shaft as well as the rotor mounting hole with a piece of cloth and place the rotor on the motor sha
13. gh the centrifuge housing is restricting the temperature rise of the samples to the standard value of 40 C with each rotor even at maximum speed Imbalance In case of unequal loading of opposite buckets tube racks or tube holes the operation will be interrupted during the acceleration phase The rotor will he decelerated to a standstill Additionally the message ERROR appears on the preset speed display If the actual spec display shows error no 1 the difference in weight of the samples is too big Fill the tubes and load the rotor as described uncer point 3 If the actual speed display shows error no 2 there can be several reasons The inbalance switch is not adjusted correctly The imbalance switch is defective 7 1 Service and inspection of centrifuge Centrifuge service and inspection should be done regularly and only by authorized and qualified personnel Use only original spare parts Maintenance and cleaning Maintenance The maintenance of the centrifuge involves essentially keeping the rotor chanber the rotor and the accessories clean Please pay special attention to anodized aluminium parts Breakage of rotors can be caused even by slight damages In the case of rotor buckets or tube racks contacting corrosive liquids the respective spots and parts have to be cleaned carefully Corrosive liquids are for example alkaline soup solution
14. owing rules must be strictly adhered to Do not operate the centrifuge if it is not installed correctly Never operate the centrifuge in a disassembled state e g without sheet metal cover Do not run the centrifuge if electrical or mechanical systems have been tampered with unauthorized persons Never use accessories such as rotors and buckets which are not exclusively approved by Hermle Labortechnik GmbH or National Labnet Company exot camercially available centrifuge tubes of glass or plastic Do not spin corrosive samples which may cause damage to the centrifuge and impair the mechanic resistance Never operate the centrifuge with rotors or buckets which show any signs of corrosion or mechanical damage The manufacturer is only responsible for the security and reliability of the centrifuge if The unit is operated according to the instruction manual Modifications repairs and new adjustments are performed by HERMIE or Labnet authorized persons and the electrical installation of the location where the centrifuge is operated corresponds to the IHC regulations Description Mocel Z 233 M2 is a microcentrifuge Four rotors for microcentrifuge tubes are available 24 x 1 5ml 44 x 1 5ml 48 combo 24x1 5 24 x O 5ml and 64 x 05m1 The Z 233 M2 is microprocessor controlled and has a maintenance free induction drive motor Do not operate the centrifuce i
15. rting the centrifuge quick key Lid key To qoen the centrifuge lid Tf the green IED of the lid key is ht the lid is closed correctly Quick key For short time runs Start key To start the centrifice Stoo key To stop the centrifuge befor the preset operating time has expired or to stop the centrifuge during a continuous run Starting the centrifuge The rotor has to be fixed correctly and completely loaded Close the centrifuge lid As soon as the green IED of the Lid key is lit the centrifuge can be started Then press the start key quick key Short time nns For short centrifuge runs you can start the run with the quick key Press the quick key The centrifuge starts and keeos running as long as you press the quick key The operating time will be indicated in seconds on the digital time display Stop key Press the stop key if you want to interrupt a centrifuge nn The centrifuge decelerates according to the adjusted brake intensity You can change the brake intensity during deceleration a Temperature Features Temperature During centrifugation heat is generated by air friction between the rapidly spimning rotor and the air inside the rotor chanber The temperature rise depends on the rotor swing out or angle rotor bucket type ambient temperature running time and the speed of the rotor The continous air flow throu
16. uld scratch the protective coating of the rotor If the rotor chanber has not been properly cleaned this will produce a fine black dust which can cause significant damage to the centrifuge chamber rotor buckets and the samples Disinfection If for example through tube breakage infectious material is spilled into the centrifuge the rotor and the rotor chamber should be disinfected Rotor is autoclavable Rotor and rotor chamber should then be treated with a neutral disinfection agent for example 10 bleach solution A disinfectant spray should be used to thoroughly clean the chamber rotor adapters etc amp Breakdown Emergency lid release In case of power failure or any malfunction the lid can be opened manually to protect your samples Please proceed as follows Unplug the power cord There is a plastic plug at the left si of the certrifire housing Behi lastic plug there is a red coord tic plug and pull the red cord The lid can then be opened 8 2 Check list Trouble shooting The error message will be indicated by an error mmber on the digital speed display sce figue 11 There is a distinction between two different kinds of errors The digits on the indication speed have the following meaning Error no 1 49 Forced stop If oe of those errors cocurs the rotor will be braked from the preset speed to 0 As
17. urs number of hours symbol m for number of minutes press start minutes running time indication end continuous run Attention to check the qperating time you have to wait one minute after start You can stop a continuous run with the stop key 4 6 Preselection of brake intensity and acceleration With the fast slow key 1 you have the choice between two steps of brake and acceleration intensity rpm speed Post ref slow Figure 8 If the green fast control lap is lit the unit accelerates and cecelerates fast If the green slow control lap is lit the unit accelerates and c amp celerates very softly below 2 000 rpm Acceleration times fast slow in seconds Rotor C0200 95 18 x 1 5mL 10 29 9 2 Rotor C0230 2A 24 x 1 5m1 10 20 15 26 Rotor C0230 43A 64 x 05mL 15 25 17 2 Rotor C0230 9A and C0230 55A 44 x 1 5ml and 24 x 1 5 24 x 05mL 15 25 17 235 Deceleration times fast slow in seconds Rotor C0200 95 18 x 1 5ml 0 13 11 18 Rotor C0230 2A 24 x 1 5m1 13 23 15 28 Rotor C0230 43A 64 x 05mL 16 27 19 34 Rotor C0230 9A and C0230 55A 44 x 1 5ml and 24 x 1 5 24 x 05mL 16 27 19 3 The numbers in brackets for 220V 50 60Hz Non brackets numbers for 120V 50 60Hz 4 7 Keyboard Sta
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