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Digital PicoView User Manual: PV Acquire 1.6x

1. NEW OBJECTIVE amp PICOVIEW NANOSPRAY SOURCES DIGITAL PICOVIEW NANOSPRAY SOURCE SOFTWARE MANUAL MODEL DPV 550 PV ACQUIRE 550 1 6X PV CONFIGURE 150 550 1 1 NEW OBJECTIVE 2 Constitution Wav Woburn Ma 01801 1023 USA 800 220 2998 US toll free 781 933 9560 phone 781 933 9564 fax www newobjective com DIGITAL C PICOVIEW DIGITAL PICOVIEW NANOSPRAY SOURCE SOFTWARE MANUAL FOR MODEL DPV 550 PV ACQUIRE 550 1 6 PV CONFIGURE 150 550 1 1 2009 New Objective Inc All rights reserved No portion of this document may be repurposed or reprinted without the prior written consent of New Objective Inc Table of Contents 1 Introduction l 1 Software Overview 1 1 Prior to Installation 1 1 2 Read this First 2 1 Document Conventions 2 1 Safetv Considerations 2 2 Prior to Installation 2 2 Handling Fused Silica Tubing 2 2 Tip Adjustment and or 2 2 Replacement The Transfer Line 2 2 Trademarks 2 3 Limited Warrantv 2 3 Version 2 3 3 Svstem Components 3 1 Host PC Svstem Requirement 3 1 Compatible Operating Svstems 3 1 Software Checklist 3 1 Hardware Checklist 3 1 TABLE OF CONTENTS IVANVW 5 4 TVLIOIG DIGITAL PICOVIEW SOFTWARE MANUAL TABLE OF CONTENTS 4 Software Installation 4 1 PV Acquire Software Installation 4 1 5 Software amp Hardware Initialization 5 1 Connectin
2. ON OFF Divert Ch Figure 8 2 Toggle Digital Divert ON Digital Divert Divert Enable Oorr Divert Channel 1 Divert Input Channel Divert Channel 0 Channel 0 S Divert When Input is True Closed Switch _ Figure 8 3 Digital Divert configuration PIN 1 CONTACT CLOSURE 5V CH PIN 2 CONTACT CLOSURE IN PIN 3 DIGITAL IN CH 1 PIN 4 DIGITAL IN CH 1 45V plug B Figure 8 4 Digital Divert Interface Box 8 2 8 3 Digital Divert Digital Divert is a powerful feature that enables control of the Digital PicoView source from a third party hardware platform Control is via low level interface either a traditional contact closure or digital TTL compatible control signal The interface enables remote toggling of the XYZ stage position between the operat ing and retract positions Another instrument mass spectrometer autosampler liquid chromatograph etc can then be used to control whether the nanospray emitter is in the op erating spray position or the retracted rinse position The combined system ensures that both the mass spec inlet and emitter stay as clean as possible during repetitive LC MS MS runs For example some LC s provide a so called Run out signal that is actively true when a gradient is being delivered This can be used to enable Digital PicoView to position the emitter in the spray position du
3. sees amnes Increasing exposure time wil decrease frame rate Increasing gain will allow for a shortor exposura timo but will nctoace imago noice mage Preview Arculsiin Loop Time rs Apply Laon Time wil decrease image rete but will increase the ns Apply ov of pang ho stage covers er postion Off meene thet updates ies update merit posti Fol te ste tesa vl Us sage conte cix dir Chanya tam default setings oniy foptial tubo or lens is changod Knob Controls Appl Defeats the manual knob contiols on the X Y Z Stags Figure 7 2 PV Acquire Settings Tab 7 2 1 Settings Tabs The Settings tabs found at the top of the main window offer three levels of con trol Settings Source Image and Diagnostics Figure 7 2 Main Window Button Feature Comment Source Image Tab Exposure Gain Apply Controls Camera Exposure Time and Image Gain Source Image Tab Image Magnification Sets the Digital Image Magnification Value Selects Cursor Mouse Pan Source Image Tab Image Tool Mouse Zoom modes Sets image shift values Source Image Tab Pan X Pan Y when using Image Mag Values of 2 or 3X Settings Tab Acquisition Loop Time Controls refresh rate of source image from camera default value of 102 mS Settings Tab Stage Pol
4. and asks if update to Settings file is desired Store Tab Save Pos File Saves a position file to disk which contains the current XYZ Stage and cursor positions Store Tab Load Pos File Loads a previously saved position file and moves the XZY Stage and Cursors to saved positions Go To Tab Direct X Direct Y Direct Z Sets the coordinates for direct stage control Go To Tab Retract amp Home Tab Apply Retract X Retract Moves the Stage to position defined by Direct X Direct Y Direct Z Sets the coordinates of Y Retract Z the Retract Position Moves the Stage to the Retract Position Retract amp Home Tab Retract Tip defined by Retra t X Retract Y Retract Z Retract amp Home Tab Home Stage Performs Home Stage Operation 7 PV ACQUIRE APPLICATION 7 5 IVANVW 5 A3IAOOId 1VLIOIG DIGITAL PICOVIEW SOFTWARE MANUAL 7 PV ACQUIRE APPLICATION EX Figure 7 3 Camera exposure setting too high Figure 7 4 Camera exposure optimized 7 6 7 3 Image Settings Exposure Gain Acquisition Time The camera acquisition controls enable complete control over image quality enabling optimal spray inlet or emitter imaging If the Exposure setting is too low the Source Image will appear black If the setting is too high the image will be washed out Figure 7 3 Figure 7 4 depicts adjustments set for optimal ca
5. 2 Connect the USB cable from the USB to serial converter to the host PC Follow instructions to activate USB driver if required Figure 5 2 5 2 Setting a Port Assignment for USB to Serial Adapter The USB to serial adapter will be assigned a virtual COM port number by the operating system This number needs to be determined and is used by the PV Configure application to establish a default settings file for PV Acquire 1 Open the Windows Device Manager In Windows XP this is typically found under Start 2 Control Panel classic view System Hardware Tab See Figure 5 3 Click on the Device Manager button 3 The Device Manager List will open Click on Ports COM amp LPT Look for Prolific USB to Serial Comm Port in the sub list See Figure 5 4 4 Note the COM port setting e g COM3 COMA etc that will be seen in parenthesis this assignment will be needed when running PV Configure for the first time 5 3 Selecting an Alternate COM Port OPTIONAL If the COM port setting chosen by the operating system is in conflict with an other device the COM number may be assigned manually This may resolve serial port conflicts 1 Right click on the Prolific USB to Serial Comm Port device in the Device Manager list and select Properties from the pop up menu Figure 5 5 2 Click on the Port Settings tab Found New Hardware Wizard This wizard helps you install software for MATRIX VISION mvBlueF X kemel driver versi
6. Always click Update Manual Pos whenever you manually adjust any of the XYZ actuators Failure to do so will result in unpredictable stage move ments It is critical that each knob control is in the central detent position after L N making a manual stage move If the knob control is left slightly off center the stage will continue to slowly move and may result in a tip crash IVANVW 5 M3I1AODId 1VLIOIG DIGITAL PICOVIEW SOFTWARE MANUAL 7 PV ACQUIRE APPLICATION Knob Controls Defeats the manual knob controls on the X Y Z Stage Figure 7 12 Manual stage knob control panel Tune Store GoTo Retract amp Home Retract X L R 2500 Home Stage Retract Y l 0 2500 Retract Z U D Retract Tip 2500 Figure 7 13 Retract amp Home panel 7 11 Using Automated Stage Polling Use of the manual XYZ stage knob controls requires the user to click on the Update Manual Pos so that PV Acquire knows the resulting stage position If the user strongly prefers to use knob controls rather than user stage control com mands for the XYZ stage forgetting to perform Update Manual Pos is problematic Mixing the knob controls and stage control could result in unpredictable stage movement when the arrow buttons are pressed To circumvent this situation PV Acquire can periodically poll the XYZ stage for its current position automatically 1 Click on the Settings tab Click and hold the Poll
7. Out L R 5805 79 Z Up Down 2433 14 Stage Retracted Crash Limit Step Size um 2 12500 Out 500 Digital Divert Divert Enable pivert Channel 0 Divert Input Channel Channel 0 Sx 1 w i OFF Divert Channel 1 Divert When Input is True Closed Switch 7 1 Figure 8 5 Stage Control and Digital Divert operations 8 3 IVANVW 5 M3I1AODId 1VLIOIG DIGITAL PICOVIEW SOFTWARE MANUAL 8 8 4 ADVANCED FEATURES 8 3 4 Digital Divert M Control Buffer The Digital Divert M interface input contains a memory buffer that will toggle the stage position for every change in command state from false true Undesired changes in channel input state should be avoided especially if they occur on a time scale that is shorter than the transit time of the XYZ stage between positions When Digital Divert is activated all stage controls are grayed out and de activated Additionally if manual knob control was activated this is also automatically turned off to prevent any unintentional stage movement 9 Emitter Positioning Procedures PV Acquire M combined with the mechanical design advantages of the magnetic interlock stage system of the Digital PicoView source enables a high level of productivity Positioning a new emitter with incredible precision and reproduc ibility has become rapid and facile with the option of manual or automated software control Built in software and manual limits minimize op
8. Retract Position Repeat steps 3 4 until the final desired position is obtained 6 Go to the Tune tab of the Mode panel and click Restore Tip to return XYZ stage to the operating position 7 13 Setting Home Position The Home Position is defined at the 0 0 0 setting of the XYZ stage The Home Position is an absolute reference position that both PV Acquire and the motor ac tuators use in making XYZ stage movements A Home Position operation must be performed when the Digital PicoView source is used for the first time A Home Position operation must also be performed if the electrical power to the motor actuators has been disrupted or disconnected If you are merely re starting PV Acquire and the power to the Digital PicoView source has remained intact a Home Position operation should not be required 1 Click on the Retract amp Home tab of the Mode panel 2 Click the Home Stage button The stage controls will gray out during the homing operation 3 When the homing operation is finished the Stage Position will read 0 0 0 and a dialog window will appear confirming that the stage has been homed Figure 7 15 The current operating position of the XYZ stage will be lost when the home position operation is performed 7 PV ACQUIRE APPLICATION Stage Position X Left Right 12005 8 Y In Out 2805 81 Z Up Down 1433 12 Stage Retracted Figure 7 14 Stage Position settings I Prompt User for In
9. Stage Position Every OFF Knob Control ON is useful for manual source tuning Cal X Cal Y Default Values of 10 7 for each 5 Click Save to save the settings file A standard dialog window will open The file may be saved under any name to any user selected directory 6 2 6 PV CONFIGURE APPLICATION 6 Note the file location and file name Click the OK button to save the file The PV Configure window will close automatically Configure PV Acquire 1 1 Digital Pico iew for 150 500 550 New Objective Inc Copyright 2005 2007 S P IC EW www newobiective com Chosen Serial Port for X Y Z Stage Control Direct Goto X L R Y IO 2 0 0 COM Port TASRLIQ INSTR com 10 6000 XYZ Stage A ssi n String written to File Corresponding COM Port Retract Position 5 To assign the USB adapter port go to Device Manager gt Ports X L R 2 Y I O 2 0 0 found in Control Panels System Hardware 2500 12500 16000 Exposure uS Acquisition Loop Time mS m je demm 2 5000 200000 5000 ag Eb Camera Gain dB Settings D 5 10 15 20 Increasing exposure time will decrease frame rate Increasing gain will allow for a shorter exposure time but will increase image noise Increasing Acquisition Loop Time will decrease image frame rate but will increase the performance of other Applications Poll Stage Position Every s Knob Control XYZ Stage i Knob Control
10. This is necessary to stop further movement of the actuator Repeat steps 7 and 8 with the Y In Out and Z Up Dn axes actuator knob controls until the emitter has reached a rough approximation of the desired operating position Click the Update Manual Pos button on the Tune tab of the Mode panel Select the Settings tab in the main window De activate the Knob Controls by changing it from On to Off Click Apply 9 1 2 2 Automated Fine Emitter Positioning using the Stage Control Panel 1 2 In the Stage Control panel set the Step Size to 100 um Fine tune the Y axis In Out emitter position by clicking on the In button or the Out button depending on which direction the emitter needs to be moved in the Stage Control panel until the emitter reaches its operating position Repeat the above fine tune procedure for the X axis Left Right and the Z axis Up Dn until the emitter is in the desired position for operation Visually verify the proper Z axis emitter position relative to the inlet by physi cally manually looking at the instrument inlet Adjust the camera focus so emitter visualization is clear Place the inlet measurement cursor blue at the center of the mass spec inlet Refer to Section 7 7 Using Measurement Cursors Place the emitter measurement cursor green at the tip of the emitter Refer to Section 7 7 Using Measurement Cursors Record the position of the emitter green cursor relative to the inlet blue curso
11. XYZ coordinates of the Stage Position in the Stage Control panel into the Retract X L R Retract Y l O and Retract Z U D input boxes The Retract Position is now saved Select the Restore Tip button on the Tune tab in the Mode panel The XYZ stage will restore the emitter to the most recent XYZ Stage Position the spray position Select the Retract Tip button in the Tune tab of the Mode panel or in the Retract amp Home tab of the Mode panel to move the emitter from the spray position back to the saved Retract Position Tune Store GoTo Retract amp Home Retract X L R 2 2500 Home Stage Retract Y 1 0 2500 Retract 7 U D Retract Tip Jj 2500 Figure 9 3 Retract amp Home tab EMITTER POSITIONING PROCEDURES 9 7 IVANVW 5 A3lIAOOId 1VLIOIG DIGITAL PICOVIEW SOFTWARE MANUAL 9 9 8 EMITTER POSITIONING PROCEDURES 9 4 Getting a New Emitter to a Previous Spray Position Reproducing a previous spray position with a new emitter is one of the many benefits of Digital PicoView While a spray position can easily be reproduced a new emitter will require a new XYZ Stage Position to reach the same spray position as a previous emitter This discrepancy in Stage Position values is a result of the manual aspect of loading a new emitter into Digital PicoView hard ware Reproducing a previous spray position is entirely dependent on using the measurement cursors and Cursor Readout available th
12. a high level of productivity for nanospray enabled mass spectrometry The user in terface supplies an interactive digital image of the MS inlet and the necessary controls for XYZ stage positioning Advanced features such as Digital Divert enable the remote control of emitter XYZ position from other instruments such as autosamplers or liquid chromatographs One of the most useful features of the Digital PicoView platform is the smart XYZ motor controls used for stage actuation Each controller is an independent mi croprocessor driven device The Digital PicoView platform can be used without the PV Acquire application It also ensures that the stage position and spray per formance will not be effected if PV Acquire is exited unexpectedly PV Acquire can be readily re started without effecting the stage position See Section 7 for use of the manual stage controls Interface Feature Performance Section Main Window Choose Settings Source 71 Image or Diagnostics Measurement User drag and drop placement Cursor for distance measurement and es tablishing reference positions Cursor Readout Shows X V distance mm be tween cursor centers Image Controls Real Time Camera Exposure 7 3 7 4 7 5 Gain Image Magnification and Image Panning controls Stage Control Panel Buttons enable precise XYZ 7 6 jogging of stage Digital Divert M Panel Remote contact closure or digital input switching between spray and r
13. be installed and the host PC rebooted prior to making the USB connections between the Digital PicoView Camera XYZ actuators and host PC PV Acquire Software Installation Locate the Digital PicoView Software Installation CD Insert the CD into the host PC CD ROM drive The CD contains an autorun application cdstarter exe that will start up with the dialog box at right Accept the dialog box by clicking YES The start up window appears Click on 1 USB to Serial Install to install the USB to serial converter driver Follow the default installation instructions Click 2 Camera Install to install the camera drivers Follow the default instal lation instructions Click 3 PV Configure Install to install the PV Configure application Follow the default installation instructions Click 4 PV Acquire Install to install the PV Acquire application and National Instruments Run Time Engine Follow the default installation instructions Restart re boot the host PC N The host PC must be rebooted to complete software installation Do not connect the USB cables from Digital PicoView Hardware prior to reboot ing as this will casue software failure 9 Two Digital PicoView applications have been installed in the Windows START menu Digital PicoView PV550 Acquirel 6 and PV Configure 10 A successful installation will also result in two icons being added to the desk top These icons point to applications for digital camera diagn
14. controllers to determination the current stage position Click EXIT every time to quit PV Acquire Always click on the EXIT buttion when closing PV Acquire Closing the vf software using other methods may leave memory resources unavailable to other applications 7 10 Using Manual XYZ Stage Knob Controls The manual XYZ stage knob controls are an effective way to drive the emitter tip to the desired spray position They also enable stage movement when PV Acquire is not running 7 PV ACQUIRE APPLICATION 1 Click on the Settings tab 2 Click on the Knob Controls switch to turn it on blue on gray off and click Apply Figure 7 12 3 Each knob control of the X Y or Z actuator can be turned clockwise or counter clockwise from a central detent position to move the stage The speed of movement is proportional to the degree of turning away from the detent position Clockwise rotation extends the motor actuator counter clockwise rotation retracts the motor actuator During movement the moving actuator yellow LED will turn on 4 Once the stage is in the desired position adjust the knob controls to the central detent position The detent position can be verified by making sure the yellow LED on each actuator motor is off Green indicates power is ON and is normal 5 Go to the Tune tab of the Mode panel and click the Update Manual Pos button This ensures that PV Acquire is aware of the newly established stage position
15. 5 1 IVANVW 5 M3I1AODId 1VLIOIG DIGITAL PICOVIEW SOFTWARE MANUAL 5 SOFTWARE amp HARDWARE INITIALIZATION Device Manager Fie Acton Heb omsaesmsma 9 0 Computer Dikdives Display adapters DID CD ROH diver i Floppy disk contoers B Human Interface Devices GIDE ATAJATAPI cortrolers 8 22 Imaging devices Keyboares at ther poring devices HM Processors Scund video ard game controllers Storage volumes 8 2 Syste devies Figure 5 4 Prolific USB to Serial Comm Port COM3 Properties PS General Port Settings Driver Details Bits per second Data bits Parity None Stop bits 1 _ Elow contro None Restore Defaults Figure 5 5 Advanced Settings for COM3 E UseFIFO buffers requires 18550 compatible UART Select lower setings correct connection problems Select higher settings fo faster per Fleceive Bullet Low 1 1 Trane Butler Low 1 06 COM Pot Number cows Figure 5 6 5 2 3 4 5 6 Click the Advanced button Choose an alternate COM Port Number from the menu list Figure 5 6 Click the OK button in the Advanced Settings window Click the OK button in the Properties window 6 PV CONFIGURE APPLICATION 6 PV Configure Application PV Configure must be run once p
16. LIOIG DIGITAL PICOVIEW SOFTWARE MANUAL 9 9 4 EMITTER POSITIONING PROCEDURES 9 1 2 Emitter Positioning Using a Combination of the Manual Knob Controls and Automated Stage Control Commands Employing a combination of manual and automated positioning a new emitter can be precisely positioned rapidly Using the manual knob controls allows you to rapidly move a new emitter into the field of view of the camera for gross positioning For more information on operating the manual knob controls refer to section 7 10 Using Manual Knob Controls of this manual Once the emitter is visible the position of the emitter can be fine tuned using the Stage Control panel to control the stage position and the Step Size until the emitter has reached its desired destination for ESI MS data acquisition 9 1 2 1 Emitter Positioning using the Manual Knob Controls The manual knob controls allow for rapid tip positioning but must be used with CAUTION It is necessary to return the knob controls to their detent position once the destination has been reached to halt movement of the stage Following completion of manual adjustment of the stage the new manual position of the XYZ Stage must be refreshed in the software by clicking the Update Manual Pos button Failure to do so will trigger inaccurate software control of the XYZ stage and potential emitter damage If no change in the Stage Position values is ob served a communication error has occurred and must be in
17. Stage Polling On Off Determines the frequency of polling the stage controllers for position Off means that updates occur only when the user presses update manual position Polling the stage frequently will decrease the response of the user interface stage controls Cal X Cal Y S ave Settings Screen Cursor fo iii g Calibration o File Change from default settings only if optical tube or lens is changed Figure 6 2 6 3 IVANVW 5 A3IAOOId 1VLIOIG PV CONFIGURE APPLICATION 6 TVONVW 5 4 1VLIOIG 6 4 7 PV Acquire 550 Application 7 1 PV Acquire Start up PV Acquire requires user input of the location of the Configuration File each time it is started The XYZ stage also requires a Home Stage operation if it is the first use of the stage or if the power to the stage motors has been interrupted or disconnected The Home Stage operation sets the 0 0 0 point for XYZ stage motion Operation without establishing the proper Home position will result in erratic stage positioning and a loss of range of motion 1 Choose PV Acquire from the Start menu under the Digital PicoView heading A dialog window asking for the location and name of the Configuration File created in section 6 2 will appear Choose the File and click OK Figure 7 1 A second dialog window will open asking if the XYZ stage requires a Home Stage operation The Home Stage operation is require
18. Stage Position Every s drop down menu Select a polling interval time of 5 10 or 30 seconds recommended qc o Click Apply You may verify automated polling operation by manually adjust ing the stage position using one or more knob control s and waiting for the Stage Position window to update vf Using a short stage polling time 5 or 10 seconds can result in poor perfor mance of the PV Acquire user interface on certain host PC s since portions of the interface are unavailable during the update position operation 7 12 Setting Retract Position The Retract Position is typically used in two different operations 1 Moving the emitter far from the MS inlet making it easier to change emitters or clean the inlet etc 2 Establishing the emitter position for use with the Digital PicoView rinsing station The XYZ stage is retracted through the Retract Tip button from the Tune or Retract amp Home tab of the Mode panel The actual XYZ stage position of the Retract Position is set on the Retract amp Home panel Figure 7 13 1 Click on the Retract amp Home tab of the Mode panel 2 Clickthe Retract Tip button The stage will move to the settings set by Retract X Retract Y and Retract Z values The Stage Retracted LED will light up 3 Note the position of the XYZ stage If a different Retract Position is desired type the new value s into Retract X Retract Y and or Retract Z 4 Click on Retract Tip to move to the new
19. UAL 6 PV CONFIGURE APPLICATION of the user interface The default value yields a frame rate of approximately 8 10 images sec X Choose Serial COM Port e Cursor Calibration Cal X Cal Y Values 58 Sets the mm calibration value in um pixel of the on screen Value should ONLY be changed from default settings if the optical tube or Tenant tr tei eat lens is changed from standard configuration Cal X and Cal Y default values are 10 7 um pixel Figure 6 1 UNS Multiple PV Configure settings files may be used Each user can have one or more settings files to accommodate different nanospray configurations e All of the settings with the exception of the COM port value may be changed directly from within PV Acquire 6 2 Creating A Configuration Settings File 1 Select PV Configure from the Windows START menu under the Digital PicoView heading A dialog box asking you to select the proper COM port will pop up Figure 6 1 2 Select the COM port number from the menu selection as reported by the Device Manager in section 4 2 In this example the selected COM port is COM3 The main PV Configure dialog window is now available 4 Select and modify each of the desired configuration parameters Guidelines for typical set up Direct Goto amp Retract default values are good starting points Camera Settings Exposure 20 000 5 Gain 10 dB Acquisition Loop Time 102 mS Poll
20. age If this occurs start the Windows Task Manager ctrl alt del locate and end the PV Acquire 1 exe process Repeat the process in Section 5 2 Setting a Port Assignment to determine the proper COM port assignment 10 4 PV Acquire User Interface Frozen The user interface can freeze or lock up if too many mouse clicks occur be fore the program can respond This is typically problematic when the camera s Acquisition Loop Time parameter found under the Settings tab in the main win dow is set below 50 mS The most efficient way to fully recover from lock up is to go to the Windows Task Manager end the PV Acquire 1 exe process and re start the application It is recommended that after errors occur with PV Acquire that the host PC is rebooted to prevent any additional downstream errors from occuring 10 DIAGNOSTICS amp TROUBLESHOOTING 10 3 IVANVW 5 M3I1AODId 1VLIOIG DIGITAL PICOVIEW SOFTWARE MANUAL TECHNICAL SUPPORT Contact New Objective For Technical Support questions for your Digital PicoView or regarding columns fittings valves etc please contact the New Objective Customer Solutions Group Hours Email Telephone Fax Postal Mail Monday Friday 9 a m 5 p m ET TechSupport NewObjective com 1 781 933 9560 1 888 220 2998 U S Toll Free 1 781 933 9564 New Objective Inc 2 Constitution Way Woburn MA 01801 1023 USA NEW OBJECTIVE 2 Constitution Way Wo
21. burn MA 01801 1023 888 220 2998 Sales 781 933 9560 Tel 781 933 9564 Fax www newobjective com XD DPV550H Rev 0209
22. d if this is the first use of the stage or if power to the stage motors has been interrupted or discon nected Figure 7 2 A Home Stage operation must be performed when the Digital PicoView stage is used for the first time OR if power to the unit s XYZ stage has been disrupted or disconnected When the Home Stage operation is finished a dialog window will appear notifying the end user Once the stage is fully initialized and PV Acquire has established communi cation with the XYZ stage motors a confirmation dialog window will appear Figure 7 3 This message only appears at statup however not each time the stage is homed 5 Click OK in the message box to begin using PV Acquire 7 PV ACQUIRE APPLICATION Open Configuration File B Documents Computer Network Places Documents ument Configuration Desktop My Document My Computer File name PV Settings My Network Files oftype AN Fies C Figure 7 1 Renumbering Stage Units Left Right is First Unit In Out is Second Unit Up Down is Third Unit Home Stage Answer Yes if first use or power was out Figure 7 2 Stage is initialized and Ready to Run Figure 7 3 7 1 IVANVW 5 M3I1AODId IVLIDIQ DIGITAL PICOVIEW SOFTWARE MANUAL 7 7 2 PV ACQUIRE APPLICATION 7 2 Overview PV Acquire is a powerful user interface for Digital PicoView enabling
23. drop mouse operation using the left mouse button They are a powerful tool for establishing long term repeatable emitter positioning Typically the blue cursor is placed at the position of the mass spec inlet and the green cursor is placed at the emitter position The Cursor Readout will then show the precise distance between the inlet and emitter To position a cursor 1 Position the mouse cursor over either screen cursor The mouse cursor will change shape from a solid to hollow cross Note the blue cursor is a center marked line and should be grabbed at the center mark 2 Left click and hold the mouse while dragging the screen cursor to the de sired position 3 Release the mouse button The Cursor Readout will show an updated distance measurement between the two cursors Figure 7 9 7 8 Stage Control Commands amp Parameters You can easily control XYZ position of the emitter tip using the Stage Control commands in the upper right corner of your screen The Stage Control contains two fields and six positioning commands Figure 7 10 Crash Limit defines the maximum forward tip Y distance um before colliding with the mass spec inlet While the default limit is 12 500 you can customize the value for each emitter Step Size is the incremental distance traveled by the tip when you press any of the Stage Control buttons 7 PV ACQUIRE APPLICATION Stage Stage Control Position In X Left R
24. e Control Panel Fine Automated Emitter 9 3 Positioning Using the Stage Control Panel Emitter Positioning Using a 9 4 Combination of Manual and Automated Stage Controls Emitter Positioning Using 9 4 Manual Knob Controls Automated Fine Emitter 9 5 Positioning Using the Stage Control Panel Setting the Crash Limit 9 6 Parking an Emitter 9 6 Setting an Emitter 9 7 Rinse Position Getting a New Emitter to a 9 8 Previous Spray Position 10 Diagnostics Troubleshooting 10 1 No Image Visibile in 10 1 Video Window Serial Connection Failure 10 1 Incorrect COM Port Assignment 10 2 PV Acquire User Interface Frozen 10 2 TABLE OF CONTENTS IVANVW 5 A3IAOOId TVLIOIG TABLE OF CONTENTS TVONYW 34VML14OS M31AODId gt 1 Introduction Welcome to the Digital PicoView Software Instruction Manual This manual describes the installation and operation of the PV Acquire Software module to be used with Digital PicoView hardware from New Objective Inc In addition to being a useful reference this manual can be utilized during user training Storing this manual near the computer operating your Digital PicoView is highly recom mended 1 1 Software Overview The Digital PicoView software package consists of two main modules The first is PV Configure which is used to set up and configure a default settings file The second is PV Acquire which is the application that pro
25. er in a similar fashion as outlined in section 9 1 Positioning a New Emitter This can be approached using either of the two techniques outlined in section 9 1 1 or 9 1 2 9 3 1 Setting the Emitter Rinse Position 1 2 3 4 11 12 14 15 Select the Settings tab in the main window Activate the manual Knob Controls bv changing it from Off to On Click Apply Using the manual knob control on the Y axis actuator In Out turn the knob control from the detent or neutral position cpunterclockwise to move the emitter position out from the mass spectrometer inlet Using the manual Knob Control on the X axis actuator Left Right turn the knob control from the detent or neutral position counterclockwise to move the emitter right towards the rinse station Using the manual Knob Control on the Z axis actuator Up Down turn the knob control from the detent or neutral position clockwise to move the emitter position up towards the dripper outlet Click the Update Manual Pos button on the Tune tab of the Mode panel Activate the dripper so liquid is flowing constantly Check the alignment of the emitter position with the direction of liquid flow Proceed with fine tuning the emitter position using the Stage Control panel as outlined in section 9 1 2 2 until the emitter is in the desired wash position Select the Retract amp Home tab in the Mode panel In the retract amp Home tab of the Mode panel enter the
26. etract rinse position Mode Panel Choose Tune Store Go To or Retract amp 7 1 2 Home Tabs for In depth control features Main Window Settings Source Image Dignostics tabs Measurement Cursors Blue Inlet Green Emitter Cursor Readout Image Controls M PicoView Acquire 1 5 file Edit Operate Tools DG Window Help 7 PV ACQUIRE APPLICATION Source Image Diagnostics Stage Stage Control Position In xiefmign UP L Y In Out Crash Limit 0 J 12500 out Z Up Down 0 Stage Retracted Step Size um Digital Divert Divert Enable OFF Divert Input Channel Divert Channel 0 Chamel vf Divert When Input is Divert Channel 1 True Closed Switch T 1 Image Magnification Image Tool OX O3X select cursors Digital Pico View for 500 550 New Objective Inc Copyright 2005 2007 www newobjective com Figure 7 1 Primary PV Acquire window Tune Store Retract Tip Go To Retract amp Home Restore Tip EXIT Update Manual Pos Press Exit Before Quitting Program Stage Control Panel Digital Divert Panel Mode Panel Tune Store Go To Retract amp Home tabs 7 3 IVANVW 5 A3IAOOId 1VLIOIG DIGITAL PICOVIEW SOFTWARE MANUAL 7 7 4 PV ACQUIRE APPLICATION
27. for working with Digital PicoView Helpful tips provide shortcuts or guidelines for best practice Indicates a warning or essential step to avoid future error or complication Indicates potential for injury by electric shock Indicates an extremely hot surface Indicates an operation requiring eye safety protection Indicates an operation requiring protective gloves poeppba Indicates an operation using fragile material s 2 2 Safety Considerations Electrospray ionization ESI involves the use of potentially lethal high voltage electrical current Observe all manufacturers safety recommenda tions in the use of such equipment No equipment modifications should be made except by trained personnel using methods approved by the manu facturer in accordance with all safety requirements Installation of equip ment should be performed by qualified personnel in accordance with all applicable electrical codes 2 READ THIS FIRST 2 1 TVONYW 33 VAA14OS M3I1AODId TVLISIG DIGITAL PICOVIEW SOFTWARE MANUAL 2 2 2 READ THIS FIRST Never use this product with defective damaged or faulty equipment Seri ous injury or death could result Only qualified personnel should use this product Provide a safe workplace equipped with all necessary safety equipment 2 2 1 Prior to Installation Follow all safety recommendations of the equipment manufacturer s All sys tem voltages must be brought to grou
28. g USB cables 5 1 USB Port Setting Determination 5 1 Choosing an Alternate COM Port 5 1 6 PV Configure Application 6 1 Overview 6 1 Creating a Configuration Settings File 6 2 7 PV Acquire 550 Application 7 1 PV Acquire Start up 7 1 Overview 7 2 The Settings Tab 7 4 The Mode Tab 7 5 Image Settings 7 6 Setting Image Exposure Time HS 7 6 Setting Image Gain dB 7 6 Setting Acquisition Loop Time mS 7 6 Image Magnification 7 7 Image Tools 7 7 Image Panning with the Mouse 7 8 Image Zooming with the Mouse 7 8 Panning Scrollbar 7 8 Saving Images 7 9 Using Measurement Cursors 7 11 Stage Control Commands amp Parameters 7 11 Tune Tab Commands 7 12 Using Manual XYZ Stage Control Knobs 7 12 Using Automated Stage Polling 7 14 Setting Retract Position 7 14 Setting Home Position 7 15 Quitting PV Acquire 7 16 Restarting PV Acquire 7 17 Advanced Features 8 1 Go To Page Direct Coordinate Movement 8 1 Store Page Commands 8 1 Digital Divert 8 2 Configuration Choices 8 2 Activating Digital Divert 8 3 Testing Digital Divert 8 3 Digital Divert Control Buffer 8 4 TABLE OF CONTENTS IVANVW 5 4 TVLIOIG DIGITAL PICOVIEW SOFTWARE MANUAL z TABLE OF CONTENTS 9 Emitter Positioning Procedures 9 1 Positioning a New Emitter 9 1 Automated Emitter Positioning 9 2 Using Stage Control Commands and Parameters Gross Automated Emitter 9 2 Positioning Using the Stag
29. ight UP Do 0 Y In Out 0 Z Up Down 0 Stage Retracted Crash Limit Step Size um 1 12500 500 Figure 7 10 Stage Control panel IVANVW 5 M3I1AODId TVLIOIG DIGITAL PICOVIEW SOFTWARE MANUAL 7 PV ACQUIRE APPLICATION Tune Store Go To Retract amp Home Retract Tip Restore Tip Update Manual Pos Figure 7 11 Tune tab in the Mode panel The L and R arrows Left Right move the tip along the source X axis by an incre ment defined by the selected Step Size and Out arrows move the tip along the source Y axis by an increment defined by the Step Size The Up and Down buttons move the tip along the source Z axis by an increment defined by the Step Size Stage Position reports the current stage position Stage Retracted LED turns on green when the stage is in the Retract Positon 7 9 Tune Tab Commands Tune tab of the Mode panel contains basic emitter actuator positioning functions and allows you to properly exit PV Acquire Click the Tune tab in the Mode panel Figure 7 11 Click Retract Tip to move the emitter to the Retract X Retract Y Retract Z coor dinates as set on the Retract amp Home tab of the Mode panel Click Restore Tip to move the emitter to the last established position before the retract button was pressed Click Update Manual Pos to establish communication between PV Acquire and the XYZ stage motor
30. l to move the stage forward on the Y In Out axis towards the inlet 16 Continue adjusting the emitter position on the Y axis till the emitter is in line with the green emitter measurement cursor 17 Adjust the emitter position by using the XYZ controls in the Stage Control tool box until the emitter is aligned with the green emitter measurement cursor The new emitter is now in the same spray position as your previous emitter 9 EMITTER POSITIONING PROCEDURES 9 9 IVANVW 5 A3IAOOId 1VLIOIG DIGITAL PICOVIEW SOFTWARE MANUAL 9 EMITTER POSITIONING PROCEDURES 10 10 Diagnostics amp Troubleshooting The error detection features of PV Acquire M enable rapid trouble shooting of software and or hardware problems Errors are detected either through pop up dialog windows or in the Diagnostics tab of the main window Recovery from an error generally requires correcting the problem quitting from PV Acquire and re starting the application Fortunately recovering from such errors is often simple and does not require changing the emitter position or interruption of the ESI voltage The unique au tonomous nature of the motor actuators of the Digital PicoView source ensures a crash of the PV Acquire software will not negatively affect your MS acquisition 10 1 No Image Visible in Window 1 In the Settings tab of the main window Check the Exposure 20 000 5 typi cally and Gain control 10 dB typically setti
31. ling Controls how often PV Acquire communicates with Stage Controllers Used when lots of manual knob positioning is used If the default value is off user interface may be come sluggish on some PC s Settings Tab Knob On OFF Controls whether the XYZ Stage Motor controller knobs are active or inactive for manual positioning Settings Tab Cal X Cal Y Cursor measurement calibration values typically these are NOT changed from default values Diagnostics Tab CCD Camera Communication and er ror values with Camera Diagnostics Tab Cursor Displays Raw Cursor informa tion used for recalibration of Cal X Cal Y Values Diagnostics Tab Configuration File Path File path of the Configuration Settings File chosen at start up Diagnostics Tab Com Port Assignment Value of the COM Port assignment 7 2 2 Mode Panel Advanced Feature Controls Mode Panel Button Feature Comment Tune Tab Tune Tab Retract Tip Restore Tip Moves stage from Current Position to the Retract Position as defined on the Retract amp Home page Moves stage from Retract Position to last used Current Position Tune Tab Update Manual Pos Forces a read and update of the XYZ Stage position Used after a manual knob driven stage move Tune Tab EXIT Preferred way to quit PV Acquire applica tion Properly releases all memory resources
32. m Out Figure 7 6 Image Tools drop down menu 7 7 IVANVW 5 M3I1AODId 1VLIOIG DIGITAL PICOVIEW SOFTWARE MANUAL 7 PV ACQUIRE APPLICATION E kom Pan X E Figure 7 7 Pan X and Pan Y scrollbars 7 8 7 5 1 Image Panning with the Mouse Pan converts the crosshair cursor to a hand to allow manual image panning Mouse panning position is reset each time the magnification is changed 1 Select Pan in the Image Tools dropdown 2 Place the hand cursor over image display 3 Click and drag to pull desired image components into view 7 5 2 Image Zooming with the Mouse Zoom In and Zoom Out functions allow image display expansion and contraction 1 Select Zoom In from the Image Tool dropdown 2 Click in the image display 3 To return to the initial magnification click Zoom Out from the Image Tools dropdown 7 5 3 Panning Scrollbar The Pan X and Pan Y scrollbars in the lower left corner of the Source Image tab provide horizontal and vertical scrolling respectively Panning enables you to move the observable image space at 2X and 3X magnifications Unlike the mouse panning option Pan X and Pan Y scrollbar settings are saved when image magnification settings are changed 1 Select either 2X or 3X from Image Magnification 2 Click the arrow on the Pan X or Pan Y slot and drag to the desired position see Figure 7 7 vf The Pan Zoom In and Zoom Out options from the mage Tools dropdo
33. mera exposure 7 3 1 Setting Image Exposure Time 15 Exposure controls image quality with respect to image illumination just like the exposure time of a digital or film camera 1 Place the mouse over the arrow on the Exposure uS scale and drag to the desired position 15 000 20 000 us works in most cases Figure 7 5 2 Click Apply and note changes in image brightness Continue adjustment to attain desired brightness contrast 7 3 2 Setting Image Gain dB Gain controls image enhancement contrast range and noise level Increasing the gain allows for a shorter exposure time e g used to image spray dynamics but increases image noise 1 Click the arrow on the Gain dB scale and drag to the desired position 8 10 dB works in most cases Figure 7 5 2 Click Apply and note changes in image quality Continue adjustment to attain desired image clarity 7 3 3 Setting Acquisition Loop Time mS Acquisition Loop Time controls the image refresh rate A low setting 50 mS can be used to monitor spray dynamics and is useful to observing transient spray behavior Settings below 100 mS can compromise the response time of the PV Acquire user interface and possibly lead to interface lock up 1 Click on the Settings tab in upper left corner of the screen 2 Click the arrow on the Acquisition Loop Time mS scale and drag to the de sired position About 100 mS works in most cases 3 Click Apply and note changes in image q
34. mitter is in the camera field of view and is close to the desired position relative to the MS inlet 9 1 1 2 Fine Automated Emitter Positioning Using the Stage Control Panel 1 2 In the Stage Control panel set the Step Size to 100 um Fine tune the X axis emitter position by clicking on the L Arrow button or the R Arrow button depending on which direction the emitter needs to be moved in the Stage Control panel Repeat the preceding fine tune procedure for Y axis and the Z axis until the emitter is in the desired position for operation Visually verify the proper Z axis emitter position relative to the inlet by physi cally manually looking at the instrument inlet Adjust the camera focus so emitter visualization is clear Place the inlet measurement cursor blue at the center of the mass spec inlet Refer to Section 7 7 Using Measurement Cursors Place the emitter measurement cursor green at the tip of the emitter Refer to Section 7 7 Using Measurement Cursors Record the position of the emitter relative to the inlet using the Cursor Readout Your new emitter is now properly positioned for ESI MS analysis 9 Stage Position X Left Right 0 Y In Out 0 2 Up Down 0 Stage Retracted Stage Control In Up Dn L R Crash Limit Step Size um 2112500 Out J 500 Figure 9 1 Stage Control panel EMITTER POSITIONING PROCEDURES 9 3 IVANVW 5 M3I1AODId 1V
35. mitter to this position This function should be used with caution since once a stage movement is initiated it cannot be stopped until executed 1 Click on the Store tab 2 Enter Direct X Direct Y and Direct Z coordinates specifying the position to which you wish to move the XYZ stage 3 Click on Apply to move the stage to the position you specify vf Direct X Direct Y and Direct Z go to coordinates on the Go To page is a good place to store the last known optimal spray position for a given emitter 8 2 Store Commands Store tab in the Mode panel contains functions for storing and re loading stage and cursor position files you select The position and cursor information is stored as a date and time stamped text file The name of the file may be changed to a title selected by the user 1 Click on the Go To tab 2 Click Save Position to save the current emitter position and cursor setting in a position file 3 Click Load Position to load a position file that will restore the cursor and stage to the position contained in that position file Once the file is loaded the cursors will move to the saved position and the stage will move to the new coordinates 8 ADVANCED FEATURES Tune Store Go To Retract amp Home Save Position Load Position Figure 8 1 8 1 IVANVW 5 M3I1AODId TVLIOIG DIGITAL PICOVIEW SOFTWARE MANUAL 8 ADVANCED FEATURES Divert Enable Divert Ch jw
36. modules included with your Digital PicoView system The Crash Limit allows the user to control the maximum Y In Out axis travel distance in correlation to the manual positioning of a new emitter Due to this aspect it is important to set and validate the Crash Limit for each and every new emitter 1 Follow the instructions highlighted in Section 9 1 1 Automated Emitter Positioning using Stage Control Commands and Parameters or Section 9 1 2 Emitter Positioning using a Combination of Manual Knob Controls and Automated Stage Control Commands to move the new emitter within prox imity of the inlet 2 Note the position of the emitter relative to the inlet Note the value for the Y In Out axis in the Stage Position indicators of the Stage Control panel 4 Setthe Crash Limit in the Stage Control panel accordingly 9 3 Parking an Emitter Parking an emitter allows the user to move the emitter from its designated spray position to a second saved position denoted in the software as the Retract Position This second saved position can be used for a variety of applications most notably for washing the exterior of the emitter in between sample injections by using the dripper feature of the Digital PicoView Once the XYZ coordinates of the Retract Position have been determined and programmed into the software it can be used reproducibly for the same emitter Finding the XYZ coordinates of the Retract Position will require positioning the emitt
37. n how the emitter is manually mounted into the Tip Holder or Coated Tip Modules CTM included with the Digital PicoView source Setting your Image Magnification to 1X allows for the largest camera field of view This provides easier location of the emitter relative to the mass spectrometer inlet for rapid and facile positioning 9 EMITTER POSITIONING PROCEDURES 9 IVANVW 5 A3IAOOId 1VLIOIG DIGITAL PICOVIEW SOFTWARE MANUAL 9 9 2 EMITTER POSITIONING PROCEDURES Optimizing the XYZ Stage position for a new emitter can be executed using one of the following two procedures fully automated emitter positioning 9 1 1 or a hybrid of manual and automated positioning 9 1 2 9 1 1 Automated Emitter Positioning Using Stage Control Commands amp Parameters Using the Stage Control panel located on the Source Image tab of the main win dow a new user can easily adjust a freshly mounted emitter to its desired operat ing position in a fully automated fashion The Step Size designation in the Stage Control panel allows for a wide range of movement 10 um to 5000 um ensuring tight control for fine tuning and maximum flexibility for easy emitter positioning It is important to wait until the stage has completed movement after each mouse click made to advance stage movement in the Stage Control panel before pro ceeding Rapid clicking of the Stage Control buttons may prompt a software fail ure and or crashing of the emit
38. n the spray position vf The desired operating spray and retract rinse positions should be fine tuned before activating the Digital Divert M feature 8 3 2 Activating Digital Divert M 1 From the main PV Acquire window select the ON Digital Divert radio but ton Figure 8 2 The Mode panel will gray out preventing access to other PV Acquire features Select Channel 0 or Channel 1 from the Divert Input drop down menu Fig 8 5 Select True or False from the Divert When Input is drop down menu Digital Divert is now ready for operation gv Suy e Digital Divert may be turned off at any time by clicking the OFF radio button 8 3 3 Testing Digital Divert To test basic operation connect two 6 insulated wires with ends stripped 1 2 bare between pins 1 and 2 of the Digital Divert Control Interface Box Set Digital Divert ON and set to Channel 0 with positive True Twist the two wires into electrical contact The Channel 0 LED should light green If the Channel 0 LED does not light check to see that the Control Interface Box power supply is plugged in The XYZ stage should begin a movement to the retract position The Stage Retract LED will then light green Disconnecting the wires should cause the Channel 0 LED to go dark with a re turn of the XYZ stage to the last used operating position 8 ADVANCED FEATURES Stage Stage Control Position In X Left Right UP Dn 12005 8 Y In
39. nd potential and all high voltage contacts disconnected from the inlet system before installation of the Digital PicoView system Inspect all equipment carefully prior to use Any damaged chipped or cracked components should not be used and must be discarded or repaired 2 2 2 Handling Fused Silica Tubing Handling of fused silica or glass tubing and tips can result in serious per sonal injury Use safety glasses or goggles meeting ANSI Z87 1 1989 re quirements or the equivalent Puncture and chemical resistant gloves should also be worn at all times 2 2 3 Tip Adjustment and or Replacement Do not attempt to adjust or replace the tip unless the ESI high voltage and other applicable voltages are turned off and are at ground potential N Reduce anv applicable backing pressure liquid gas etc to ambient be fore loosening ESI tip fittings and removing the fused silica tip or transfer line from the coupling union Prior to pressurization make sure that com ponents are tightened to specifications to prevent separation during use Failure to adhere to this warning could result in projectile like expulsion of the tip from the coupling union which could cause serious personal injury or damage to surrounding apparatus 2 2 4 The Transfer Line The transfer line connecting to either a tip module or the Micro Injection Valve must not be made from an electrically conductive material It must be fabricated from an electrically i
40. ngs for non zero values 2 Check the Diagnostics tab If the camera is properly connected to the host PC all error codes should read zero All handle OUT variables will have positive values If the camera connection has failed the error code Dev Open or error code Get Dev will read back a negative value Driver Handle OUT will read zero Check the connection cable then quit and restart PV Acquire Simply reconnecting the camera cable without re starting PV Acquire will not correct the problem 10 2 Serial Connection Failure A failure of the serial connection such as unplugging the cable to the XYZ stage controls or a power failure to the stage motors will result in a pop up error mes sage showing a VISA session lost message Check all stage motor cable connections and motor power supply Correct any disconnects quit and then re start PV Acquire DIAGNOSTICS amp TROUBLESHOOTI NG Figure 10 2 Diagnostics tab showing all camera connections are stable Figure 10 3 Diagnostics tab showing USB cable interruption 10 1 IVANVW 5 M3I1AODId IVLIDIQ DIGITAL PICOVIEW SOFTWARE MANUAL 10 DIAGNOSTICS amp TROUBLESHOOTING Figure 10 1 Diagnostics tab 10 2 10 3 Incorrect COM Port Assignment Choosing an incorrect COM port assignment in PV Configure M will result in painfully slowand repetitive at least six pop up window error messages similar to the following showing a VISA Timeout Expired mess
41. nsulating material such as PEEK or fused silica Otherwise the operator may be exposed to potentially lethal voltage In systems where high voltage is applied directly to the ESI tip the liquid sample inside the tip and transfer line tubing is also raised to a high voltage To prevent exposure to potentially lethal voltages a suitable ground point for the liquid inside the line must be provided 4 Do not operate Digital PicoView without the external housing and lid in place Do not defeat any mass spectrometer system software or hardware safety interlocks 2 3 Trademarks The following trademarks are found in this instruction manual Teflon is a registered trademark of du Pont de Nemours and Co MicroTight and SealTight are trademarks or registered trademarks of UpChurch Scientific Inc a division of IDEX PEEK and PEEKSsil are trademarks of Victrex plc LabVIEW is a trademark of national Instruments Windows Windows 2000 Windows NT Windows XP Windows Vista Paint and PowerPoint are trademarks or registered trademarks of Microsoft Corporation PicoView Digital PicoView PicoTip PicoFrit IntegraFrit SilicaTip TaperTip GlassTip PicoClear and PicoTip Powered are trademarks or registered trademarks of New Objective Inc All other trademarks are the property of their respective companies 2 4 Limited Warranty This software under draft release bears no warranty whether implied or other wise 2 5 Ve
42. on 2 If your hardware came with an installation CD SS or floppy disk insert it now What do you want the wizard to do Install the software automatically Recommended O Install hom a list or specific location Advanced Click Next to continue Figure 5 1 Found New Hardware Wizard Welcome to the Found New Hardware Wizard Windows will search for current and updated software by looking on your computer the hardware installation or on the Windows Update Web site with your permission Bead our pivacy polic Can Windows connect to Windows Update to search for software Yes this time only O Yes now and every time connect a device No not this time Click Nest to continue Figure 5 2 Svstem Properties System Restore _ Automatic Updates Remote General Computer Name Hardware Advanced Device Manager The Device Manager lists all the hardware devices installed 3 on your computer Use the Device Manager to change the properties of any device Drivers E Driver Signing lets you make sure that installed drivers are compatible with Windows Windows Update lets you set up how Windows connects to Windows Update for drivers Driver Signing Windows Update Hardware Profiles Hardware profiles provide a way for you to set up and store different hardware configurations Hardware Profiles pply Figure 5 3
43. ostic software These pointers may be deleted from the desktop at no risk 11 Additional Installation Notes are available on the CD ROM follow these directions should the cdstarter exe application fail to run 4 SOFTWARE INSTALLATION cdstarter 1 Welcome to the 550 Installation Package No Confirm installation DPY550 Installation Package 1 USB to Serial 3 PV Configure Install Install Ez B 2 Camera Install 4 PV Acquire Install Installation Notes Startup dialog box Ka Pv 550 Acquiret_s E MATRIX VISION A EB National Instruments Two Digital PicoView applications will be added under the Windows START menu myDeviceCo wxPropView Two additional icons will be added to the desktop upon installation These are not required and may be deleted without risk 4 1 TVONYW 33 VAA14OS M3IAODId SOFTWARE INSTALLATION 4 TVONYW 5 4 TVIIOIG 4 2 5 SOFTWARE amp HARDWARE INITIALIZATION 5 Software and Hardware Initialization 5 1 Connect the USB cables Prior to using PV Acquire the USB connections between the host PC and Digital PicoView source need to be established This will be followed by the creation of a default configuration file using the PV Configure application 1 Connect the USB cable from the digital camera to the host PC Follow in structions to activate USB driver if required Figure 5 1
44. portunities for damaging fragile emitter tips Saved positions allow you to divert between a programmed spray position and a programmed Retract Position The following procedures will highlight how to execute each procedure and the benefits and limitations of each The Crash Limit is one of the software safety features and must be set properly 9 2 In order to properly set the Crash Limit a new emitter must first be posi tioned using one of the outlined procedures 9 1 Focusing the camera on the MS inlet allows the camera focus to function as a reference of the emitter position in the Z axis Up Down plane similar to the green and blue Measurement Cursors for the relative positioning of the emitter to the MS inlet Once the camera focus is finely tuned on an emitter at the center point of the inlet it will be used as verification of the emitter s Z axis position for bringing a new emitter to a previous spray position 9 4 To mount a new emitter onto the magnetic interlock stage system follow the procedures in the Digital PicoView Hardware Manual Consult the section of the Hardware Manual for mounting PicoTip emitters and PicoFrit columns 9 1 Positioning a New Emitter While Digital PicoView combined with the PV Acquire software module allows you to have full control over and fine tune your emitter position the manual aspect of mounting a new emitter still plays a large role A new emitter will have a new Stage Position dependent o
45. put Stage is at Home Position Figure 7 15 Dialog window confirming that the stage has been reset to the home position 7 15 IVANVW 5 M3IAODId 1VLIOIG DIGITAL PICOVIEW SOFTWARE MANUAL 7 Figure 7 17 When restarting PV Acquire PV ACQUIRE APPLICATION Renumbering Stage Units Left Right is First Unit In Out is Second Unit Up Down is Third Unit Home Stage Answer Yes if First use or power was out the prompt to home the stage can be declined if power to Digital PicoView has remained on 7 14 Quitting PV Acquire The proper way to quit PV Acquire is to always click Exit from the Tune tab of the Mode panel when closing PV Acquire Closing the software using other methods may leave background processes running and memory resources unavailable to other applications While other methods such as directly closing the program window through the Microsoft Windows buttons or using the application stop button will work to terminate the program it is not a best practice with PV Acquire Using the Exit button ensures that all PV Acquire functions are ter mintated properly and that saved settings will be retained 1 Click on the Tune tab in the Mode panel 2 Click on Exit A dialog box will appear asking if you want to update the de fault settings file with the currently selected values 3 Select Yes if you wish to 1 modify the existing settings file or 2 create a new settings file with the mo
46. r using the Cursor Readout Your new emitter is now properly positioned for ESI MS analysis 9 Stage Position X Left Right 12005 8 Y In Out 2805 81 Z Up Down 1433 12 Stage Retracted Stage Control i L E R Crash Limit 112500 pe Step Size um Qut j1000 Figure 9 2 Stage Control panel EMITTER POSITIONING PROCEDURES 9 5 IVANVW 5 M3I1AODId 1VLIOIG DIGITAL PICOVIEW SOFTWARE MANUAL 9 9 6 EMITTER POSITIONING PROCEDURES 9 2 Setting the Crash Limit The Crash Limit feature of the Stage Control panel allows the user to set the maxi mum travel distance of the XYZ Stage on the Y In Out axis from Home Stage position 0 0 0 when using the automated Stage Control commands The Crash Limit feature does not apply when the manual Knob Controls function has been activated for manual emitter positioning When set properly the Crash Limit will help to minimize unnecessary and unexpected damage to emitters The maximum travel distance for all three axes is 12 500 uim from Home Stage position 0 0 0 The X and Z axes will stop movement upon reaching 12 500 with minimal opportunity for emitter damage Adjustment of the Y axis poses the greatest risk for damaging an emitter depending on the manual positioning of the emitter into the Tip Holder or Coated Tip Module see the Digital PicoView Hardware Manual for more information on these two
47. ring gradient elution and in the rinse position for loading column conditioning etc The Digital Divert interface is made available through the Digital PicoView source camera A cable and interface box connected to the camera provides the connection point for the interface Refer to the Digital PicoView Hardware Manual for instructions on making the physical interface connections and setting up the emitter rinsing station 8 3 1 Configuration Choices The Digital Divert M interface gives a choice over the type of control contact closure or TTL compatible and the polarity of the interface The type of control is determined by the selected control channel Channel Selection Channel 0 is for conventional contact closure and requires an actual contact between pins 1 and 2 of the Interface Box Figure 8 8 The input is true when the contact is closed The Channel 0 LED will light when the contact is closed Channel 1 is for a TTL level compatible digital signal The input is true when the voltage between pins 3 and 4 of the interface box is approx 2 3 2 8 V or greater 5 V The Channel 1 LED will light when the voltage is high Divert when input is True will divert the XYZ stage to the retract position when the channel is true When the channel is false the XYZ stage will be in the spray position False will divert the XYZ stage to the retract position when the channel is false When the channel is true the XYZ stage will be i
48. rior to the first activation of PV Acquire The settings file created by PV Configure is required for the operation of PV Acquire 6 1 PV Configure Overview PV Configure generates a default settings configuration file ini file that PV Acquire is required to open each time it runs Think of this much like a tune file for your digital source settings The default settings configuration file saves the following information COM port assignment Number of the COM port for communication with the XYZ Stage through the USB to serial adapter XYZ Stage Poll Stage Position On Off and Timing Controls if PV Acquire should periodically the XYZ stage for changes in manual Knob Control position Standard Value is OFF XYZ Stage Knob Control On Off Turns the manual adjustment knobs on each XYZ controller On Off Standard Value is OFF e Stage Direct Goto Position Sets the default XYZ Stage position for the retract position e XYZ Stage Retract Position Sets the default XYZ Stage position for the direct go to command Used as the retract position for Digital Divert e Camera Exposure and Gain Settings Sets start up values for image brightness and Cain Camera Acquisition Loop Time Sets the acquisition period in milliseconds for image acquisition Settings below the default value 102 mS may result in a slow down 6 1 IVANVW 5 M3IAODId 1VLIOIG DIGITAL PICOVIEW SOFTWARE MAN
49. rough PV Acquire M The inlet and emitter measurement cursors function as the X and Y controls for posi tioning the emitter relative to the MS inlet For more information on how to use measurement cursors refer to Section 7 7 Using Measurement Cursors in this manual The camera focus functions as the control for positioning the emitter in the Z plane 1 Set the manual knob controls to their detent or neutral positions 2 Deactivate the manual Knob Controls by selecting the Settings tab in the main window and changing the value from On to Off Click APPLY 4 Click the Update Manual Pos button in the Tune tab of the Mode panel Move the inlet measurement cursor blue placing it at the center of the mass spec inlet 6 Move the emitter measurement cursor green and place it at the tip of the emitter Adjust the camera focus so the mass spec inlet is clear Set the Step Size in the Stage Control panel to 2500 Click the OUT arrow in the Stage Control panel to retract the stage on the Y In Out axis away from the inlet 10 Follow the instructions in the Digital PicoView Hardware Manual to physi cally disconnect the stage from the Interface Flange and slide it back 11 Follow the instructions in the Digital PicoView Hardware Manual for replac ing an emitter 13 Relock the Digital PicoView source onto the Interface Flange 14 Set the Step Size in the Stage Control panel to 500 15 Click the IN arrow in the Stage Control pane
50. rsion This manual was written to be current with PV Configure version 1 1 and PV Acquire version 1 6x 2 READ THIS FIRST 2 3 TVANVW 5 M3I1AODId READ THIS FIRST 2 TVONYW 5 M3I1AODId TVIIOIG 2 4 3 System Components 31 Host PC System Requirements 3 1 1 PV Acquire is compatible with the following operating systems Windows Vista Window XP Windows NT Windows 2000 Optimal performance has been found on Windows XP A standard CD ROM drive is required for software installation The host PC is required to support two USB 2 0 or better connections 3 2 Software Checklist Digital PicoView Software Installation CD All of the required application software device drivers dll s and the National Instruments Run Time Engine are on this single CD Digital PicoView Operator s Manuals CD 3 3 Hardware Checklist Digital PicoView Nanospray Source with actuators amp camera installed See Digital PicoView Hardware Manual for configuration USB to Serial Port Converter Serial Cable for Connecting Actuators to Converter USB Cable for Camera to Computer connection PCI USB 2 0 Card for PC 3 SYSTEM COMPONENTS 3 1 TVONYW 5 M3IAODId TVLISIG SYSTEM COMPONENTS 3 TVONYW 5 M3I1AODId TVIIOIG 3 2 A Software Installation vf Software from the Digital PicoView Installation CD should
51. st recently used Digital PicoView operating pa rameters If you answer yes you will be prompted with a standard file dialog window 4 Theonly other way to exit the program is through the Windows Task Manager Control Panel CTL ALT DEL but this method is not recommended vf Always click EXIT before closing the PV Acquire window Closing the soft ware using other methods may leave memory resources unavailable to other applications 7 15 Re Starting PV Acquire PV Acquire can be re started with the procedure in section 7 1 However as long as the power to the Digital PicoView XYZ stage has remained intact from the last use there is no need to execute a Home Stage operation The user may safely decline the Home Stage request on re start see Figure 7 17 This way the stage position spray operation etc of the source will not be dis turbed during the PV Acquire re start sequence vf PV Acquire may be exited and re started without disturbing the physical operation stage position spray voltage etc of the Digital PicoView source provided that a Home Stage operation is NOT performed on re start 7 PV ACQUIRE APPLICATION 7 17 IVANVW 5 A3lAOOId 1VLIOIG PV ACQUIRE APPLICATION 7 TVONVW 5 M3I1AODId 1VLIOIG 7 18 8 Advanced Features 8 1 Go To Direct Coordinate Movement The Go To tab contains a dialog box to enter specific X Y Z coordinates for the emitter and move the e
52. ter into the inlet For more information on how to use the XYZ Stage Control buttons refer to section 7 8 Stage Control Commands and Parameters of this manual Before proceeding with controlling movement of the XYZ stage solely through the Stage Control panel the manual Knob Controls must be placed in their detent position and deactivated For more information on operating the manual Knob Controls refer to section 7 10 Using Manual Knob Controls Failure to do so may promote software failure and emitter damage 1 Select the Settings tab at the top of the main window 2 De activate the Knob Controls by changing it from ON to OFF 3 Click Apply 9 1 1 1 Gross Automated Emitter Positioning Using the Stage Control Panel 1 Select the Source Image tab in the main window to return you to the camera image of the mass spectrometer inlet 2 Set Image Magnification to 1X 3 Adjust the camera focus so the center point of the inlet is visible and clear 4 In the Stage Control panel set the Step Size to 1000 um 5 Move the X axis emitter position left from the Home Stage position 0 0 0 by clicking on the L arrow button in the Stage Control panel Move the Z axis emitter position up from the Home Stage position 0 0 0 by clicking on the Up button in the Stage Control panel Move the Y axis emitter position in from the Home Stage position 0 0 0 by clicking on the IN Arrow button in the Stage Control panel Repeat these three steps until the e
53. uality in the Image Preview screen on the right Continue adjustment to attain desired image clarity 1 Decreasing Acquisition Loop Time increases image frame rate but lowers performance of the user interface 7 4 Image Magnification Magnifying the Source Image to 1X 2X and 3X provides digital magnification of fine image details Radio buttons located in the lower right corner of the Source Image screen allow rapid transition from one magnification level to another 1X is the default magnification level To change magnification level click the corresponding radio button and wait for the image to refresh Figures 7 6 7 5 Image Tools Image Tools define available mouse modes when the mouse is placed over the image display Availability of Image Tools depends on magnification level At 1X magnification Select Cursor is the only active Image Tool Select Cursor transforms the mouse into a crosshair cursor allowing the user to move the green reference cursor in the Source Image display screen Additional tools activate upon selecting 2X or 3X magnifications These tools are grayed out at 1X magnification 1 Select a higher magnification level 2 Clickthe dropdown menu immediately below Image Tools and select desired tool Figure 7 6 7 PV ACQUIRE APPLICATION Exposure uS Gain dB 5000 100000 10 20 Figure 7 5 Camera Acquisition Controls Image Magnification Select Cursor Oix Ox Osx Zoom In Zoo
54. vestigated before proceeding If the Poll Stage Position function in the Settings tab of the main window is activated it will automatically update the manual position within the designated time frame Once the emitter has been positioned using the manual knob controls it is recom mended that the manual Knob Controls be deactivated so they can no longer be used for manual tip positioning If the manual Knob Controls are OFF turning the Poll Stage Position function OFF as well minimizes software delays created by the actuators communication loop and the software during the Poll Stage Position function Use the following sequence of steps to bring the emitter from Home Stage 0 0 0 position into the camera field of view manually using the knob controls 1 Select the Settings tab in the main window 2 Activate the manual Knob Controls by changing it from Off to On 3 Click Apply 4 Select the Source Image tab in the main window to return to the camera im age of the mass spectrometer inlet 10 11 12 13 Set Image Magnification to 1X Adjust the camera focus so the center point of the inlet is visible and clear Using the manual knob control on the X axis Left Right actuator turn the knob control from the detent or neutral position clockwise This will move the emitter position left towards the mass spectrometer inlet Once the emitter has reached its destination return the knob control to the detent or neutral position
55. vides the graphical user interface for the source PV Acquire enables remote stage positioning high reso lution spray imaging and advanced features such as Digital Divert This manual guides you through the installation of PV Configure and PV Acquire software and the source software communication hardware The sections guide you through the daily procedures of software initialization image optimization and tip positioning Your separate Digital PicoView Hardware Manual is your reference for source assembly hardware configuration and specific nanoelectrospray applications Additional resources for nanoelectrospray applications can be found in the Tech amp App Notes section of the New Objective website http www newobjective com resources app notes html 1 INTRODUCTION 1 1 TVONYW 5 M3IAODId INTRODUCTION 1 5 M3I1AODId TVIIOIG l 2 2 Read This First vf Software from the Digital PicoView Installation CD should be installed and the host PC rebooted prior to making the USB connections between the Digital PicoView Camera XYZ actuators and host PC 2 1 Document Conventions This manual employs several conventions to describe mouse operations soft ware functions and alert the user with important information For your refer ence these document conventions are delineated below Helpful Tips amp Precautions This manual contains notes to the user
56. wn and the Pan X and Pan Y scrollbars are only active at the 2X and 3X image magnifications 7 6 Saving Images The Source Image is readily captured with a copy and paste operation into an other application such as Windows Paint The background image and cursors will be saved the cursor distance readout however is not 1 Right clicking in the image window will bring up a pop up menu 2 Select Copy Data Figure 7 8 Use a paste operation in another suitable application such as Paint or Microsoft Powerpoint 7 PV ACQUIRE APPLICATION Description and Tip Visible Items b Clear Graph Create Annotation y AutoScale X qf AutoScale Y AutoScale Z Ignore Color Array Interpolate Color Smooth Updates Figure 7 8 Use the Copy Data feature to copy images to the clip board for use in other applications 7 9 IVANVW 5 A3lIAOOId TVLIOIG DIGITAL PICOVIEW SOFTWARE MANUAL 7 PV ACQUIRE APPLICATION Settings Source Image Diagnostics Source Cursor blue Emitter Cursor green Cursor Readout Exposure uS Gain dB Image Magnification Image Tool TT 500000 0 40 20 1X O2X OSX Select cursor x Figure 7 9 Measurement cursors provide precise reporting on distance between the two cursor positions 7 7 Using Measurement Cursors There are two measurement cursors Blue Cursor inlet cursor Green Cursor emitter cursor Cursors are placed with a drag and

Digital PicoView User Manual: PV Acquire 1.6x

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