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AZURA Assistant ASM 2.1L User Manual

1. Connecting a Device to a Computer to a Local Area Network LAN This chapter describes how to set up a local area network LAN and how a network administrator can integrate this LAN into a Assistant ASM 2 1L User Manual V6815A version 1 2 48 Note Process Note Prerequisite Procedure KNAUER Initial Startup company network The description applies to the operating sys tem Windows and all conventional routers To set up a LAN we recommend to use a router The following steps are necessary 1 On the computer go to the Control Panel and check the LAN properties 2 Connect the router to the devices and the computer On the computer configure the router to set up the network A w Install the chromatography software from the data storage device 5 Switch on the device and run the chromatography software The port ist set to 10001 at the factory The IP port numbers in the device configuration of the chromatography software must be identical to those in the device otherwise the connection fails Configuring the LAN Settings The LAN uses only one server which is normally the router from that the devices automatically receive their IP address In Windows power saving hibernation standby automatic Windows update and screen saver must be deactived In case you use a USB to COM box the option Allow the computer to turn off this device to save power in the Device Manager must be deac
2. Remote control see spring strip 24 Rotor seal Alignment 71 S Salts 8 Sample Precleaning 41 Solvents 8 Spare parts 18 Spring strip 24 Start IN 25 T Technical Support 22 Terminology 80 Test cell 7 34 Test intervals 55 Transportation damages 82 U UV light 20 V Valve 2 postion valve 15 Dismounting 68 Multiposition valve 15 Types 14 Valve drive 14 Valve position Inject 37 Load 37 WwW Warning signs 21 Warranty 82 KNAUER Assistant ASM 2 1L User Manual V681 5A version 1 2 HPLC SMB Osmometry Ih KNALER IQ for Knauer Devices IQ Installation Qualification AZURA ASM 2 1L VASM21LA Device data Device version Serial number Inventory number Knauer product number Firmware version Location Application software ClarityChrom ChromGate O Version number Place of installation Device manufacturer Wissenschaftlicher Gerdtebau Dr Ing Herbert Knauer GmbH Hegauer Weg 38 14163 Berlin support knauer net Contact Name Street Postcode City Town Telephone E mail Guarantee valid until Date of execution if applicable KNAUER 13 08 2012 ASM 2 1L Rev Q1 9 HPLC SMB Osmometry Ih KNALIER IQ for Knauer Devices Table of contents IQ Installation QUANITICATION iencodcvictcouicruuodiarerdeiuessrcesintandbeducudstaatuntuiuuudetobianedeedas 1 DIAA T EmO f e P EE A EEE EE E E E A Matadeeneneeaacece 1 Rules on reproduction and Auplication sssi
3. Connect valve with Use stainless steel capillaries with an column inner diameter of 0 25 mm Connect the column Use stainless steel capillaries with an to the detector inner diameter of 0 25 mm Connect the detector Use stainless steel capillaries with an to the waste bottle inner diameter of 0 25 mm HPG system Procedure KNAUER 1 2 3 4 5 6 7 Connect the solvent bottles with degasser Connect the degasser with pump Connect the pumps with the mixer Connect the mixer with valve Connect the valve with column Connect the column to the detector Connect the detector to the waste bottle Assistant ASM 2 1L User Manual V6815A version 1 2 46 Initial Startup Tools Tool kit A 1033 Legend Detector Pump with Smart mix Pump with pressure transducer Degasser Valve Column Fig 44 Scheme of a high pressure gradient compact HPLC sys tem Explanation Necessary Tools Connect solvent bot Connect suitable tubing material for tles with degasser the solvent to an inlet of the degas ser Connect degasser with Install suitable solvent material from pump the respective outlet of the degasser to the inlet of the pump Connect mixer with Use stainless steel capillaries with an valve inner diameter of 0 25 mm Use the outlet of the pressure transducer Connect valve with Use stainless steel capillaries with an column inner
4. bined with a fume hood or similar extraction device Legend Gas outlet Fig 42 ASM 2 1L gas outlet on the front Using PEEK Fittings PEEK fittings withstand a maximum pressure of 400 bar CAUTION Damage to the flow cell caused by strongly tightened fittings Check the torque of screw fittings 5 Nm for stainless steel fittings 0 5 Nm for PEEK fittings ASM 2 1L as Compact HPLC Isocratic System Procedure 1 Connect the solvent bottles with degasser Connect the degasser with pump Connect the pump to the manual valve Connect the column to the detector 1 2 3 4 Connect the valve with column 5 6 Connect the detector to the waste bottle KNAUER Assistant ASM 2 1L User Manual V6815A version 1 2 45 Initial Startup Tools Tool kit A 1033 Legend Degasser Pressure transducer Pump Detector Valve Column Fig 43 Scheme of an isocratic compact HPLC system Connect solvent bot Connect suitable tubing material for tles with degasser the solvent to an inlet of the degas ser Connect degasser Install suitable solvent material from with pump the respective outlet of the degasser to the inlet of the pump Connect the pump to Use stainless steel capillaries with an the manual valve inner diameter of 0 25 mm Use the outlet of the pressure transducer For informations about the assignment of the valve ports please refer to sec tion Valve
5. screw fitting Place the biconical seal on the new needle seal and turn everything into the syringe connection at the valve Screw the screw fit ting on the valve moderately tight Insert the injection syringe all the way into the new needle seal Loosen the screw fitting until the injection syringe can still be pulled out straight easily but with noticeable friction Fig 66 Biconical seal needle seal and screw fitting of the syringe connection Fig 67 Schematic display of the syringe connection Degasser If the degasser leaks solvent can reach the motor and from there reach the degasser exhaust and flow out In this case the automatic pump control will switch the device off Inform the technical support of KNAUER SmartMix Mixer in the ASM 2 1L If the flow rate and the gradient composition become unstable it may be due to a blockage of the mixing chamber The mixing chamber can be opened to change the filter system or cartridge KNAUER Assistant ASM 2 1L User Manual V6815A version 1 2 73 Maintenance and Care Opening the SmartMix Mixer Procedure 1 Loosen the four Exterior view screws on the mixer lid with an Allen wrench wrench size 3 0 x 150 2 Lift off the mixer lid Fig 68 Mixer lid Exchanging the Filter System Functional principle Several filter inserts are inserted into the SmartMix mixer in a specific order They can be renewed as needed Prereq
6. screws evenly and alternately to prevent the pump pistons on the inside from jamming Fig 54 Screw fittings on the pump head Assistant ASM 2 1L User Manual V6815A version 1 2 65 Maintenance and Care Replacing the Pump Head Depending on the requirements of the user different pump heads are used Prerequisite The pump head has been flushed with suitable solvent WARNING Aggressive or toxic solvent residue can irritate the skin Wear protective gloves Flush the pump head before exchanging it Removing the Pump Head 1 Remove the tubing from the inlet and outlet of the piston backflushing 2 Unscrew the eluent line 3 Loosen the fittings and remove the capillary 4 Loosen the opposite pairs of fastening screws on the pump head evenly and alternately 5 Hold the pump head by hand and consecutively pull out all fastening screws 6 Remove the pump head Legend Inlet and outlet of the piston backflush ing Fastening screw Inlet screw fitting Pressure transducer Fig 55 Replacing the pump head KNAUER Assistant ASM 2 1L User Manual V6815A version 1 2 66 Maintenance and Care Installing the Pump Head 1 Screw in the opposite pairs of fastening screws evenly and alternately 2 Tighten all fastening screws evenly with an Allen wrench 3 Screw in the capillary with outlet fitting of the pump head and inlet fitting of the pressure sensor and tighten with an Allen w
7. solvent enters the piston backflushing Inform the Technical Support of KNAUER Exchange the pump head Pressure and flow Pump without pressure sensor Pay rate variations attention to the influence of the pressure on the flow rate will not be compensate Purge the pump head to remove the air bubbles Always tighten the inlet screw fit tings and outlet screw fittings on the pump head with a torque wrench and 7 5 Nm Clean the check valves Exchange the check valves Exchange the pump head Inform the Technical Support of KNAUER Pump head leaks Inspect the inlet and outlet screw fittings of the pump head When the pump head seals are defective solvent enters the piston backflushing Inform the Technical Support of KNAUER Exchange the pump head Valves Valve leaks Tighten the connections Pressure variations Exchange the rotor seals caused by leaking valve head Degasser Air bubbles occur in Check the inlet and outlet fittings the solvent tubes of Replace old fittings if necessary the degasser outlet KNAUER Assistant ASM 2 1L User Manual V6815A version 1 2 57 Troubleshooting Poem Somton No eluent flow If a buffer solution stays in the degas ser for an extended period it can cause clogging Use another degasser chamber Carefully let water flow into the clogged degasser chamber to dis solve the buffer If this does not help contact the Technical support SmartM
8. 0 5 1 25 2 5 mm 1 4 1 18 1 16 250 10000 ml min stainless steel and PEEK Assistant ASM 2 1L User Manual V681 5A version 1 2 13 Device Overview U Z View Micro 1 16 Quartz 0 10 ml min 280 um glass 0 01 ml min Pump in the ASM 2 1L The ASM 2 1L can be equipped with a pump P 2 1S P 4 1S Legend Pressure transducer Pump head Fig 4 Pump with pressure transducer in the ASM 2 1L Additionally the pump P 4 1S is equipped with a pressure trans ducer Pump heads The pump heads can be easily removed and replaced via four front accessible screws by the user KNAUER Assistant ASM 2 1L User Manual V6815A version 1 2 14 Device Overview Valve Drives in the ASM 2 1L Prior to delivery the valve drive can be equipped with KNAUER multiposition valves and 2 position valves Legend Valve Fig 5 Valves in the ASM 2 1L Usage 2 positon valves are mainly used for injections Multiposition valves are used to switch fluid streams For example columns and eluents are selected and eluate are fractionated Valves Valve types There are two different types of KNAUER valves Multiposition and 2 position valves Connections and channels of 2 position valves are described by abbreviations Example 6P 3C This is a 2 position valve with 6 ports and 3 channels In case of multiposition valves the central port is not counted The valve drive depends on the number of the valve
9. 56405 eed i oes Pawan ee eee Ui eee ees 19 Definition of Personal and Material Damages dret a o se Mead nies bh eae Rae eee oe Ba aha ee Bl beh bk 20 Symbols and Signs is 4 30h lt 4 Svinte dete s od ain pedo eee EEE 21 Unpacking and Setup 2654 64 3 0cdesene eS oh eee ee Ree Se os 3 O46 BS 22 Contacting the Technical Support 0 0 ee eee eee 22 Location REQUINEINGIS Acie 5 sie th ges na EEEE E E eG EE RRR ORE eps 22 Unpacking osc ig eek ERE ASE EVAR EEA ER REA ORS FO NO RE 23 Connectors on the Rear Side 244 ve 1aviceeteveueeawstpedasatekdwss 23 Controlling with the Pin Header 2 0 eee eee eee eee 24 Plug Connector Assignments 0 eee eee eee cece eee eens 24 Integrator Output 2 c g esearo tee chew ee he na Shee eRe Cee CEES RS 26 Initial Stara e004 ch ihe ied ed th esrar ke sea e aii eE Cea eR 27 Holding Bracket rriei entut euan hiatus ttadag tenis epee teat as 27 Attaching the Holding Bracket to the Side Panel 28 Mounting a KNAUER Valve vais gai Oh ease eee NGG Bed a ea 29 Mounting a VICI Valco Valves oss 0o tao Red ee eR wR Ae he 30 Mounting a VICI Valco valve drive 2 0 eee eee ne 31 Mounting a Flow Cell cc go s 4 apace eens See Ray otk a eee wPR oles 32 Mounting Axial Compressible Columns Vertex Plus AX 20 m 30 mm 32 KNAUER Assistant ASM 2 1L User Manual V6815A version 1 2 DELGClLOR iG Stet Pie ot E eN San eee OE ee hon Os E Ge SS 33 Setting the Optical
10. Aww ORE 45 Leak Management sa bs acgcur da tye See Gaous dag gion shew ek slants 46 Connecting a Device to a Computer to a Local Area Network LAN 47 Configuring the LAN Settings ad soe eee ee SEAS REN OERERER SEEK RRR 48 Conmecting th Cables cscs sorb as eee wk where Sek ees ces 49 Configuring the Routers iiiga SaGyins 25 EWR OAH OES Ok ae Ree eeu es 49 Integrating the LAN into a Company Network 045 50 Controlling Several Systems Separately ina LAN 0 50 Startup Of the Device sapis wleoeey se Ge Wena ae aaah eae we a ate 4 51 Operation with Chromatography Software or Control Unit 52 Control with Chromatography Software 0 eee eee eee 52 Control with Control Unit tact 2 seaves Si5 ee Rees et ee CER eee 52 Meaning Of the LEDS 2 00 eson aa taie er eee ans eee eee whale ies 52 Control with Chromatography Software 20 e ee eee eee 53 Control with Control Units ss elke s SS ONS ES ENE PER ae 53 Installation Qualification IQ 0 eee eee ee eee 54 Operation Qualification OQ cc eee ee cee eee eee 54 TeStIntervals 2445 ote men ea Be Roads Sabah bint Bot ke aa a inal aed Mg ek 4 54 EXGCUUION mreana tienes seu seat a aties seh eed aagus ene bbe gas 54 KNAUER Assistant ASM 2 1L User Manual V6815A version 1 2 Troubleshooting sosseocs ioes Se Sere es BAG OSS HORE ASS oe 5S ee 85 55 System Messages eissa ee emare KARAS a RR PARR ER HERRERO E
11. Chromatography Software or Control Unit Note Left LED Center LED Right LED AZURA Malfunctioning system after repeated standby possible After repeatedly using the standby switch off the power switch and back on again to reset the data storage Color Operating condi Operation tion red Error message Check the sys tem Shortly press the switch to deactivate the error message does not light Device is switched Switch on the off device flashes green Device not ready Wait until the for measuring device is ready on Device active or ready for measur ing Device in standby Press the switch to end the standby A device can be operated in two ways Control with chromatography software Control with Control Unit Control with Chromatography Software To control the device with chromatography software it must be connected to the computer through the LAN interface Devices can be controlled with e g OpenLAB EZChrom edition version A 04 05 or higher ChromGate version 3 3 2 or higher and ClarityChrom version 3 0 7 or higher You will find a detailed description on the chromatography soft ware in the software manual Control with Control Unit You can control the device using the touchscreen on the mobile Control Unit The Control Unit is an optional accessory You will find a detailed description on the Control Unit in its accompany ing user manual KNAUER Assista
12. Fig 52 Analytical flow cell 1 Using the allen wrench loosen the threaded ring 2 Using tweezers remove the compression part or gently tap it on a clean surface 3 The lens is protected by a seal ring This must be renewed every time the lens is disassembled 4 Remove the lens and clean with a clean soft cloth or with an appropriate solvent in an ultrasonic bath 5 Afterwards assemble the flow cell and make sure that the new seal ring does not interrupt the light path 6 Using the wrench tighten the threaded ring The lens is clean If the cleaning does not have the desired effect clean the light guide Assistant ASM 2 1L User Manual V6815A version 1 2 63 Prerequisite Tools Legend Threaded ring Cover Spacer Compression bush ing Light guide with seal ring Cleaning the light guide KNAUER Maintenance and Care Cleaning the Light Guide of a Preparative Flow Cell The preparative flow cells have a rod shaped light guide instead of the concave lens of the analytic flow cells CAUTION Light splitter can get contaminated if it is being touched with bare hands Wear gloves Use a clean soft cloth Device is switched off Power plug has been pulled Flow cell has been disassembled Tweezers Allen wrench size 3 mm Fig 53 Preparative flow cell 1 Using the Allen wrench loosen the threaded ring 2 Remove the cover and the spa
13. PUMp Heads i 4 35450 23 FAC ER VES A ANS FASE A E OS ES A Hes 81 WAVES rarei ue wares EAN RAE n ges sade Sa eae gs audio Bae aa sian 82 Sample Loops for the Valve 0 2 eee ee ee eee ens 83 Holding Bracket for Valves Columns and Flow Cells 2 83 IIIT enarrat 0 s a dh clas tothe ane eae Ogee EEA M2 fou Baek ak Ravens a stats 84 Abbreviations and Terminology eee eee e eee eee 85 Legal Information 4 5 065 66 sss eee wee we ewew baw bw Re eee 87 Warranty Conditions sess 32k Bens aek tes Ged eek eee cern awe FAA 87 Manufacturer 23 eins tau kre Gouda igee ok ae eeu tee eae ees 87 Transportation Damages sic iwry sacs wutenie ieee ws eR AS w Nee 87 Disposal 5 4 4 2 035 35p acne ate RSG S d woe eo Bld do BG eee y ele deal d 88 Declaration of Conformity 265 5 8 0 05 52 6 06 S45 eee eee oS 6h Se SoH ns 89 Table of FIQUres dessaiisn as fe et oe oe ad OSS SSS Sees ees 90 IND OK cess ee SRR Pelee ake RA SIE ae OE a OE ae ES 92 IQ Installation Qualification Functional test 94 KNAUER Assistant ASM 2 1L User Manual V6815A version 1 2 7 Intended Use Note ASM 2 1L ASM 2 1L as compact HPLC Operating range Detector UVD 2 1S Pump P 2 1S P 4 1S Valve Drive V 2 1S KNAUER Intended Use Only use the device for applications that fall within the range of the intended use Otherwise the protective and safety equip ment of the device could fail ASM 2 1L is suit
14. UVD 2 1S Increased noise of the baseline and reduced sensitivity can be a result of a dirty flow cell Often it is sufficient to rinse the flow cell to restore optimal sensitivity Do not touch the lens or the optical fiber with bare hands Dirty lenses or optical fibers could falsify the measurement Wear gloves Rinsing the Flow Cell CAUTION Contamination of the flow cell caused by oil drops Do not use compressed air for drying rw runo ving solvents are recommended for flushing diluted HCI 1 mol L 1 mol L NaOH aq Ethanol Acetone Syringe 1 Fill the syringe with eluent 2 Inject it into the inlet of the flow cell and allow it to act for 5 minutes 3 Fill the syringe with water and inject again 4 Remove the flow cell from the detector and use a nitrogen stream to dry it Assistant ASM 2 1L User Manual V6815A version 1 2 62 Result Next steps Prerequisite Tools Legend Seal ring Lens Compression part Threaded ring Cleaning the lens Result Next steps KNAUER Maintenance and Care Flow cell is clean Check if the baseline noise has disappeared If the rinsing does not have the desired effect all flow cells can be disassembled to clean the lens Cleaning the Lens of an Analytical Flow Cell Device is switched off Power plug has been pulled Flow cell has been disassembled Tweezers Allen wrench size 3 00 oo
15. and switch on the front of the device 53 Analytical flow cell 2 s2 7s4 os saa bw Sek we Me Se NS ee eA 63 Preparative flow cell pox us04 3G ye Rte kee ae CES ER OR Ae EAC 64 Screw fittings on the pump head 222s eee 65 Replacing the pump head 2 0 eee eee ee ee 66 Functional principle of the check valve 0004 67 Exchange the check valves 0 cece eee eee eee eens 68 Open the valve housing 0 cece eee eee teens 69 Remove the top part of the valve cee eee eee ee 69 Deposit the rotor seal true sided with the marking of the position 69 Inner components of the valve 0 0 ee eee ee eee 70 Position of the rotor seal in the multiposition valve 71 Position of the rotor seal in the 6P 2C valve 71 Position of the rotor seal in the 6P 3C valve 71 Mounted VICI Valve on sevtsres eaten se dee eee eee sea ee s 72 Biconical seal needle seal and screw fitting of the Syringe CONNECT ON po scared see aK wales awa ew BE Vee whe eed 73 Schematic display of the syringe connection 4 73 Mixer lid 25s caeai nena e REDENE E tas eee Atos Sab e 74 Remove the filter system from the mixer lid 74 Insert new filter system in the mixer lid 0 004 75 KNAUER Assistant ASM 2 1L User Manual V6815A version 1 2 92 Index Index A Abbreviations 80 Acce
16. doubt contact the Technical Support of the manufacturer Assistant ASM 2 1L User Manual V6815A version 1 2 9 Intended Use Less suitable eluents Not suitable eluents Suitable eluents Acetone at 4 25 C 39 2 77 0 F Acetonitrile Benzene Chloroform Ethyl acetate Ethanol Hexane heptane at 4 25 C 39 2 77 0 F Isopropanol Carbon dioxide liquid 99 999 CO gt Methanol Phosphate buffer solu tions 0 5 M Toluol Dilute ammonia solu tion Dilute acetic acid 10 50 at 25 C 77 0 F Dilute sodium hydrox ide 1M Water Dimethyl sulfoxide DMSO Slightly volatile eluents Methylene chloride Tetrahydrofuran THF Dilute phosphoric acid 1 valid for the specified temperature range KNAUER Halogenated hydrocar bons e g Freon Concentrated mineral and organic acids Concentrated bases Eluents containing parti cles Perfluorinated eluents e g Fluorinert FC 75 FC 40 Perfluorinated polyether e g Fomblin Assistant ASM 2 1L User Manual V6815A version 1 2 10 Device Overview Device Overview Legend Solvent tray Degasser Pump with pressure transducer Detector Valve KNAUER Compact HPLC Isocratic By combination of the following devices a complete isocratic HPLC system is created Pump 4 1S with 10 ml pump head Detector UVD 2 1S Degasser DG 2 1S analytical 2 channel During the configurat
17. fame Order number Valves Holding bracket for KNAUER valve com A9853 plete Holding bracket for VICI Valco valves A9853 1 completely with adapter plate Holding bracket for VICI Valco valves A9853 2 completely with adapter plate Flow cells Holding bracket for KNAUER flow cells A9853 5 completely with adapter plate KNAUER Assistant ASM 2 1L User Manual V6815A version 1 2 84 Spare Parts and Accessories Mixer SmartMix mixer 350 SmartMix mixer 100 Analytical cartridge 350 ul Micro cartridge 100 ul Filter set for SmartMix A0164 1 KNAUER Assistant ASM 2 1L User Manual V6815A version 1 2 85 Abbreviations and Terminology Abbreviations and Terminology KNAUER Here you can find information on the abbreviations and termi nology used in this system manual Explanations Backflush Backflushing of columns or precolumns to sep in arate heavily retarding substances by changing the flow direction Channel The connection of two valve ports by a special form of rotor seal Dead vol The dead volume of an HPLC system is the vol u ume of the mobile phase which is required to fill all hollow spaces of the HPLC system like capillaries mixing chamber valve etc except the column The dead volume should be as small as possible Degasser module for fluids Gradient Time dependent composition of solvent mobile phase on low pressure or high pres sure side of system H Operating mode of HPLC system se
18. flow accuracy Long service life 10 ml and 50 ml pump head selectable With or without pressure transducer Optional pump heads made of titan for bio compatibility There is the valve drive V 2 18 6V 12V 16V whereby the addi tional details refer to the ports of the valves The valves fall into two categories Assistant ASM 2 1L User Manual V6815A version 1 2 Degasser Note KNAUER Intended Use 2 position valves 2 postion valves are used to inject the sample from the sam ple loop in the pumps high pressure flow so that the sample is transported to the column In case of 2 position valves at the valve drive the rotary motions are limited to positions with a rotation angle of 60 Multiposition valves Multiposition valves are used to switch fluid streams e g the selection of eluents columns or for fractionating of eluate Valve heads available in stainless steel and PEEK Valves of the company VICI Valco Instruments Co Inc can be installed The usage of a degasser in front of the pump leads to a stable base line of the chromatogram Eluents must not be degassed before they be used in the HPLC Analytical flow rate up to 10 ml min Eluents Even small quantities of other substances such as additives modifiers or salts can influence the durability of the materials The list of selected solvents was compiled based on research in the pertinent literature and is only a recommendation If there is any
19. ports The ASM 2 1L can be equipped with following valves Valve Switch Material Pressure size a stability 2 position 1 16 Stainless 300 bar valve 1 8 steel PEEK 6P 3C 6P 2C KNAUER Assistant ASM 2 1L User Manual V6815A version 1 2 15 Device Overview Valve Port Switch Material Pressure size positions stability 6 port 1 16 Stainless 300 bar multiposi 1 8 steel PEEK tion valve 12 port 1 8 12 Stainless multiposi steel tion valve 16 port 1 16 16 Stainless 50 bar multiposi steel 100 bar tion valve 2 position Valves In HPLC the 2 position valve is also used for manual injections In this case it is mounted on the device via a holding bracket and operated with a lever The reed contact detects this process i The reed contact causes a short circuit contact This short circuit a contact is set by the user e g as a starting signal for the entire ew system or as a starting signal for a measurement with the detec tor Legend Position number 6 ports with 2 chan nels Adapter plate for mounting on the valve drive 6 ports with 3 chan nels Fig 6 2 position valves with 6p 2C and 6P 3C Multiposition Valves The multiposition valve is used in chromatography for example in the following cases Selection of up to 16 different solvents Fractioning KNAUER Assistant ASM 2 1L User Manual V6815A version 1 2 16 Device Overview Column switching up to
20. the device stands horizontally Connectors on the Rear Side CAUTION Possible damage to the device caused by condensed water Allow device to acclimate for 3 h before it is taken into operation Assistant ASM 2 1L User Manual V6815A version 1 2 24 Legend Label with serial number LAN port Interface for the Technical Support Pin header Fan Integrator output Connection and power switch USB port for the Control Unit KNAUER Unpacking and Setup The USB port is located on the right side All other ports are located on the rear side of the ASM 2 1L Fig 10 ASM 2 1L rear view Fig 11 ASM 2 1L USB port for the Control unit External devices like computers fraction collectors etc can be connected in 2 different ways to the detector Control with pin header Connected to LAN within a network Controlling with the Pin Header CAUTION Short circuit hazard Turn off the device before connecting it to the pin header Pull the power plug Plug Connector Assignments Assistant ASM 2 1L User Manual V6815A version 1 2 25 Unpacking and Setup The single ports exchange start control and error signals with other devices Connection Function EV 3 Event 3 TTL output Levels passive 5 V active 0 V Pulse QV for at least 1000 ms EV 2 Even TTL output Levels passive 5 V active 0 V Pulse O
21. the laboratory director The inspection must be documented with the date DD MM YYYY KNAUER 13 08 2012 ASM 2 1L Rev IQ4 9 HPLC SMB Osmometry Ih KNALUER IQ for Knauer Devices Shipkit list of hardware and software ASM 2 1L Shipkits of Modules C Oe T L a FC a a E eee eee rs eee eae ___ eee vasmate d KNAUER 13 08 2012 ASM 2 1L Rev IQ5 9 HPLC SMB Osmometry Ih KNAUER IQ for Knauer Devices Initial startup cam Identify the device The device is correct Check transport No transport damage is aoe observed Can Check the shipkit The shipkit is complete Connect the device to The device is connected the power supply Connect the device via The device is connected patch cabel or plug in the control unit Connect tubings and Tubings and capillaries aces are connected using the power switch display lights up applicable KNAUER 13 08 2012 ASM 2 1L Rev lIQ6 9 HPLC SMB Osmometry Ih KNALUER IQ for Knauer Devices Installation Qualification Deviations Any deviations that occur must be recorded here Pe pl Pl KNAUER 13 08 2012 ASM 2 1L Rev lIQ7 9 HPLC SMB Osmometry Ih KNAUER IQ for Knauer Devices Appendices 1 Order confirmation o S 2 Deliverynote 3 List of small and wear parts Oo o 4 Delivery logbook ooo S O 5 Knauer maintenance and service authorization 6 Other documents oo S ee KNAUER 13 0
22. the module was manufactured in cal endar week 32 of the year 2010 The last five digits are the identification number for the interface card Interface Serial number Cleaning and Caring for the Device CAUTION Intruding liquids can cause damage to the device Place solvent bottles next to the device or in a solvent tray Moisten the cleaning cloth only slightly All smooth surfaces of the device can be cleaned with a mild commercially available cleaning solution or with isopropanol Maintenance Tasks for Users The following maintenance tasks are recommended by KNAUER and may be performed by the user cell or the fiber optic connectors WARNING Irritation of retina through UV light AN Concentrated UV light can leak out from the flow Switch off the device and pull the power plug Assistant ASM 2 1L User Manual V681 5A version 1 2 61 After 2000 operating hours After 5000 operating hours After 10000 operat ing hours Cleaning the flow cell Note Flushing solution Tools Procedure KNAUER Maintenance and Care Detector Check light intensity of the D2 lamp Inspect the flow cell assembly Clean the flow cell Replace the flow cell Pump Control the seals Check screw fittings for leak loss Check screw fittings for leak loss Replace the pistons Replace the check valves Check screw fittings for leak loss Replace the O rings at the plates Detector
23. tography in following cases Separation of the sample on the precolumn Separation of the sample components on different columns The sample is separated on the precolumn The further separa tion can be carried out on column 1 and be continued on col umn 2 after switching over Fig 38 Column selection with a 6P 2C valve Precleaning of a Sample by a Precolumn The precleaning of a sample is used in chromatography for example in the following cases Assistant ASM 2 1L User Manual V681 5A version 1 2 42 Initial Startup Preseparation or separation of heavily retarding substances Legend Column Detector Precolumn Valve Fig 39 Precleaning of a sample by a precolumn After the injection of the sample the substances to be separated are heavily retarded The main column is switched into the flow system after the valve has been switched over Now the sample will be transported to the main column by using a second solvent Degasser Legend Degasser Fig 40 Front view degasser KNAUER Assistant ASM 2 1L User Manual V6815A version 1 2 a Initial Startup Connecting a Solvent Hose Procedure 1 Slide the fittings onto the hose 2 Afterwards slide on the sealing ring with pointed side first Tighten the fittings by hand Fig 41 Fittings solvent hose Loosen the fittings and check if the hose fits flush with the sealing ring Next steps Use the s
24. 16 columns Legend Position number Channel between the central port and the peripheral port Adapter plate for mounting on the valve drive Fig 7 Scheme of a multiposition valve 6P Degasser in the ASM 2 1L The ASM 2 1L can be equipped with a degasser By using a degasser up to two eluents can be degassed simultaneously in a reliable and comfortable way Legend Degasser Fig 8 2 channel degasser in the ASM 2 1L KNAUER Assistant ASM 2 1L User Manual V6815A version 1 2 17 Legend Blind plug Mixer lid Inlet to the pump Mixer housing Mounting boreholes Micro cartridge in the SmartMix Outlet to the col umn Flow rate Analytical version Micro version KNAUER Device Overview SmartMix Mixer The KNAUER SmartMix mixers are solvent mixers that are suit able for high performance liquid chromatography HPLC The liquids are mixed statically in the SmartMix mixer Fig 9 SmartMix Mixer To differentiate the installed cartridges the SmartMix mixer is supplied with different blind plugs The analytical version with the analytical cartridge has a black blind plug The micro version with a micro cartridge has a red blind plug Overview Mixer 0 1 0 5 ml min lt 1 5 ml min Analytical 350 ul Assistant ASM 2 1L User Manual V6815A version 1 2 18 Scope of Delivery Scope of Delivery Note Only use original parts and accessories made by
25. 77 Technical Data Detector UVD 2 1S photometer with reference beam Wavelength range Flow cell Delivery with flow cell 10 ul flow cell with 10 mm path length Optical bandwidth Wavelength accuracy Wavelength precision Pump P 2 1S P 4 1S Conveying system Dual piston pump with main and auxiliary piston Flow rate range 10 ml pump head 0 001 10 ml min 50 ml pump head 0 01 50 ml min Maximum pressure 10 ml pump head 40 MPa to 10 ml min Imax 70 50 ml pump head 15 MPa to 50 ml min Imax 80 Flow rate accuracy 1 at 1 ml min For pumps without a pressure transducer dependent on pres sure Flow rate precision Relative standard deviation RSD lt 0 5 1 ml min KNAUER Assistant ASM 2 1L User Manual V6815A version 1 2 78 KNAUER Technical Data Valve Drive V 2 1S Operation Power supply Maximum power con sumption IP protection class Weight Switch duration of the valve Valves Material Connectors Seals Diameter valve LAN RS 232 Pin header Buttons on the device Chromatography software ChromGate or ClarityChrom External 24 Volt DC power supply 60 W IP 20 1 86 kg 150 ms Stainless steel 1 16 1 8 PEEK 1 16 1 8 1 16 1 8 Rotor seals made of VESPEL TEF ZEL POM H TF or ETFE 47 5 mm SmartMix Mixer Flow rate of SmartMix Mixer Analytical version 0 5 2 0 ml min 0 1 0 5 mimin lt 1 5 mimin Cartrid
26. 8 2012 ASM 2 1L Rev1 Q8 9 HPLC SMB Osmometry IQ for Knauer Devices Comments lh KNALIER Responsible person Authorized agent of customer Name in block letters Date Signature Name in block letters Date Signature IQ9 9 KNAUER 13 08 2012 ASM 2 1L Rev Wissenschaftliche Gera tebau Dr Ing Herbert Knauer GmbH All rights reserved The information in this document is subject to change without prior notice Translation of the original German edition of this manual version 1 2 2013 08 19 Printed in Germany on environmentally friendly paper from sustainable forests AZURA are registered trademarks of Wissenschaftliche Geratebau Dr Ing Herbert Knauer GmbH gt See up to date manuals online www knauer net downloads HPLC SMB Osmometry Wissenschaftliche Geratebau Phone 49 30 809727 0 Dr Ing Herbert Knauer GmbH Telefax 49 30 8015010 Hegauer Weg 38 E Mail info knauer net 14163 Berlin Germany Internet www knauer net www knauer net Ih KNALIER KNAUER 2013 V6815A 0 1 03 13 Koe
27. Detector Valve Column Basics Note Legend Detector Column Valve Detector KNAUER Initial Startup After switching over the valve heavily retarding substances are eluted in opposite direction fluid from the column and flushed back to the detector Fig 35 Backflushing of the column Backflushing of a Precolumn The precolumn is used in chromatography for example in the following cases Preseparation or separation of heavily retarding substances Protection of the main column Precolumn and main column are aligned in a row behind each other Fig 36 Use of pre and main column From the valve the precolumn is filled with sample through the channel 2 gt 3 From the channel 6 gt 1 the quickly dissolved sub stances on the column are seperated the heavily retarding sub stances remain into the precolumn Assistant ASM 2 1L User Manual V6815A version 1 2 41 Note Legend Detector Column Valve Precolumn Basics Legend Detector Column A Precolumn Valve Column B Basics KNAUER Initial Startup The eluent flushes the precolumn after switchover in opposite direction Fig 37 Backflushing of the precolumn After switching over of the valve the late eluting substances are flushed back from the precolumn to the detector 2 position Valve 6P 2C Column Selection The column selection with a 6P 2C valve is used in the chroma
28. ER as a company is registered by the WEEE number DE 34642789 in the German ElektroAltgerateRegister EAR It belongs to category 8 under which fall all medical devices and laboratory equipment Within the meaning of the WEEE directive all distributors and importers are responsible for the disposal of old devices End users can send their old devices which must have been manu factured by KNAUER back to the distributor the importer or the company free of charge but would be charged for their dis posal Assistant ASM 2 1L User Manual V6815A version 1 2 lh KNALIER Declaration of Conformity Manufacturer name and address ASM 2 1L Wissenschaftliche Geratebau Dr Ing Herbert KNAUER GmbH Hegauer Weg 38 14163 Berlin Germany Product number EY The device complies with the following requirements and prod uct specifications DIRECTIVE 2006 42 EC OF THE EUROPEAN PARLIAMENT AND OF THE COUNCIL of 17 May 2006 on machinery and amending Directive 95 16 EC recast DIN EN 60799 June 1999 Electrical accessories Cord sets and interconnection cord sets IEC 61010 1 2010 Corrigendum 2011 Safety require ments for electrical equipment for measurement control and laboratory use Low voltage directive 2006 95 EC EN 61000 3 2 2005 A1 2008 A2 2009 Electromagnetic compatibility EMC Part 3 2 EMC standard 2004 108 EC IEC 61326 1 2006 Electrical equipment for measurement control and la
29. Ih KNALIER feura Assistant ASM 2 1L User Manual V6815A Hee 1 et __ lla z Table of Contents Note For your own safety read the manual and always observe the warnings and safety information on the device and in the manual Intended Use iera irar e Ses Gd ae oe oe Bee new e eel wh a ein tae a Ak a a s 7 Features of the Individual Micro Devices 0 eee eee eee eee 7 EIGENS cic sannat eane ae VS Fe a Rae ad Oa Ne ny aise ee ae 8 DEVICE OVERVIEW saas nok ei aa eek sea ees Pas 10 Compact HPLC Socratic ans oa ese sa a e a teh als ees a A E AS 10 Compact HPLC for High Pressure Gradients 0000s ee eee 10 Device Overview in the ASM 2 1L 2 0 kee ee ee teens 11 Detector in the ASM 2 7 Leo cae ite eh ghd pak Ke Ree Se eS 12 Pump I thE ASM 2b ee oo ae are ROKR a Nawag nh Mae Ree ae sounded 13 Valve Drives in the ASM 2 1L ia tb 5 3 Mead eee 3 en hed VERS ER etka 14 Valves 2504 fete ncaa Au Marae ene AA Ae taal E Meee alae ORT RE s eye acu thos 14 J POSIMOM VAlVES 3 x ant us eee eG hee ceils eh oh iy ee eee heey ahs 15 Multiposition VaIVGSyiiisnes Gary wae eke BG PaO eas eS ESI RES 15 Degasser in the ASM 2 1L 6 rs4 645 Se G4 SG ae Se OE SS ee 16 SmartMix Mixer vi ttheon te tots eile eaaa Be gf Bas a a bytes Be ohh 17 OVEIMIEW Mixer 2 5 atica nats eae eee Gard nae WE eb ee tones eats Ree ales 17 SEODE OF DELIVERY inn aoa oars eh ek eee Ge wet ow a eae 18 Safety f r Users
30. KNAUER or a company authorized by KNAUER ey ewanwneme o permene fo pramena EE m fo comarne om O i o E ewoo o e cmmcmewe ean eooo o E O Pump Exhaust KNAUER Assistant ASM 2 1L User Manual V6815A version 1 2 19 Safety for Users Safety for Users Professional group The user manual is adressed to persons that have qualification as chemical laboratory technician or comparable vocational trai ning The following knowledge is required Fundamental knowledge of liquid chromatography Knowledge regarding substances that are suitable only to a limited extent for use in liquid chromatography Knowledge regarding the health risks of chemicals If you do not belong to one of these professional groups under no circumstances may you perform the work described in this user manual What must be taken into All safety instructions in the user manual account The environmental installation and connection specifications in the user manual Observe national and international regulations pertaining to laboratory work Original spare parts tools and eluents made or recommen ded by KNAUER More safety relevant information is listed in alphabetical order in the following table Decontamination Contamination of devices with toxic infectious or radioactive substances poses a hazard for all persons during operation repair sale and disposal of a device All contaminated devices must be properly
31. M 2 1L edr VIEW 0 2 ces SPSS ARRAS EEL Ras Coke eeeee ASM 2 1L USB port for the Control unit 4 Valve installed laterally on the device 000 e eee eee Bore holes to attach holding bracket on the side panel Holding bracket is attached 22 eee eee ee nee Front side of KNAUER valve at the holding bracket Back side of KNAUER valve at the holding bracket Back side of VICI Valco valve at the holding bracket Back side of VICI Valco valve at the holding bracket Back side of VICI Valco valve drive at the holding bracket Back side of VICI Valco valve drive at the holding bracket Flow cell with adapter plate at the holding bracket Column holder with holding bracket 2 20005 Preparative flow cell 303 25 h a ne scared pas Shas eed aes Removing the slide wen bit poy ene were eee bee es sah sea gas Assembling the flow cell 0 0 0 eee ee ee eee eee eee Capillary MUNG 22 esre ia een ee a bak wa ha Dark eae ates Capillary and flow GEllisc5 jis ey he Ut atees ales ate ane gee ean aU Connections for 2 position valve 2 2 eee ee eee Valves in the ASM 2 1L ccc 5 043 eS NCA oak RRS EE ER REA Valve position LOAG vaeh i hain ee eme eee baleen wwe ye eel ea Valve position Inject jure evs kas eee eee ad Bae Ae aaa Column selection Usage of Column A 022 eee eee Column selection Usage of Colum
32. Path Length 0 cee eee ee eee 33 Inserting the Flow Cell scc2 3aun sGade eee es aGeedwehse ed ees aeuba 34 Connecting the Capillaries 0 074 0 wieeae SAGES EARS CAE SRR ORES BOWER 35 Bleeding the PUMP iss desarme ta Ra eyed REET ORR RES ERIE 35 Connecting a 2 position Valves va sae s See ee ee Re Sees 36 Application Examples for Valves 02 ee ee ee eee eee ees 36 2 POSILION valve 6P 3C secede settee dais eee so gia th kh ce be esau ee 37 COlUMNSElECHON senses resres da i eae teh dowels eee eh aes 38 Backflushing of a Collins ese e354 4a Hae Ree OEE ed Eee eRe RESET ES 39 Backflushing of a Precolumn 2 2 0 cee eee eee eee eee ees 40 2 position Valve 6P 2C 35 5 6 bles ae Sc he EGS GORE Ree To ioe se Rae BEES 41 COlUMM SECON aseni a EE E E EEEE aera cae 41 Precleaning of a Sample by a Precolumn 0c ee eee eee eee 41 DEGASSED men de dvd Bee he Saw wean eg wg ee ae ew Ge 42 Connecting a Solvent Hose ee ee ee ee ee eens 43 Connecting Degasser and Pump ee eee eee ee eee eee 43 Meaning of the Degasser LEDs cece eee ee ee eee eees 43 Degasser Gas Outlet prere seu oie ese Owe wae wh aR ee ws aes 44 Using PEER PINGS 2 54 5 otek hae RS ER RR PP EGR OER OS RTE ROR 44 ASM 2 as Compact HPLG gag o Me we ta ee eh ae SEEGER EEA RRA 44 ISOCTAUIE Systemi Sitesi nak Ge es Sew kat TAKEN POA Oecd Naat Mele eae 44 HPG System araras niai G ha ea Hee REE PA wae Re EADE
33. RS 57 Maintenance and Cares i scsi oo ese eed a woe See SHEE OWENS 60 Maintenance Contract vig ets Gand So Satin Nine aerate oe taal Wah eat oe gare 60 Required System Information 02 eee ee eee 60 Cleaning and Caring for the Device eee eee eee eee eee 60 Maintenance Tasks for Users v2 goae ofa was ee P EREDAR OReR Oe SEEDER A 60 Detector UVD 2 15 scan we pak BOE e eee eee he Ok Oe ee eee eas 61 Rinsing the Flow Cell g yu scoundrel Ste Mee ee 61 Cleaning the Lens of an Analytical Flow Cell 000 62 Cleaning the Light Guide of a Preparative Flow Cell 63 Pump P ZIS PATS 4 rt sek anwnk aGta kes Oa e Ke aca eee eek aes 64 Tightening the Screw Fittings 15 0s2 dane awa eee Se Gaede eee aes 64 Loosening the Fittings sca 6 venice a posed ews Oe ee a 64 Replacing the Pump Head 4 4 03 ss wsec ewe ee Bee we Baw ee gy 4 65 Removing the Pump Head 2 444 sk Oiees Ae Soe CRIA TRS BARS ee hed 65 Installing the Pump Head sac sees es eG wk ee ee eee whee 66 Exchanging the Check Valves 0 0 e eee eee ee ee eee eees 66 Removing the Check Valves 0 cece eee eee eee eens 66 Cleaning the Check Valves 2 4 lt iki wens kOe mb ke bd REAR Oe eS 67 Installing the Check Valves j 0 suss oases Oks HSS ROK AWR OR Gade 67 V le rerea are font ces ad laa fa rales ares Pactra tated Doe EA ET Cava ds Diao 67 Removing the Valve cxncg nu Pace alee ake a eae OS eae EEE dee 67 Alignmen
34. V for at least 1000 ms t 2 __ Relay contact EVI The contact is on a floating basis Its Event 1 setting depends on the settings in the Control Unit or software Steady rate signal passive open relay contact o a active closed relay contact 6 Pulse Closed relay contact for at least f 1000 ms Permissible load of the relay contact 1 A 24 V DC START IN TTL input Low active Secure switching threshold at least 10 mA After receiving a signal short circuit to ground from an external device the device starts If controlled with soft ware an electronic trigger is send through the LAN ERROR IN TTL input Low active Secure switching threshold at least 10 mA After receiving a signal short circuit to ground from an external device an error message appears and the device stops KNAUER Assistant ASM 2 1L User Manual V6815A version 1 2 26 Unpacking and Setup 5 V Provides a voltage of 5 V with respect to GND This makes it possible to sup ply a consumer that is switched by an EVENT Max current 50 mA Reference point of the voltage at the signal inputs Event controlled switching of 24 V against GND Max current 200 mA Reference point of the voltage at the signal inputs Integrator Output The integrator output sends measuring signals from the detec tor KNAUER non bipolar 1 channel 0 5V DAC 16 bit Scalable Adjustable to offset Assistant ASM 2 1L Us
35. able for the liquid chromatography The ASM 2 1L is a multifunctional device which can be configured with up to three micro devices by the user Up to three micro devices of a total of five different micro devices for the HPLC High performance liquid chromatography can be assembled into an ASM 2 1L The micro devices in the ASM 2 1L are con trolled with chromatography software or optional Control Unit With a suitable selection of micro devices the ASM 2 1L can be used as a supplementary module for the HPLC or it can be com bined to a compact HPLC system for chromatographic analyses in the high pressure range The device can be used in the following areas Biochemistry analyses Chemical analyses Food analyses Pharmaceutical analyses Environmental analyses Features of the Individual Micro Devices Following devices can be integrated A test cell is preassembled to the detector Before the detector can be used the test cell has to be replaced by a flow cell Single channel detector with variable wavelength With the deuterium lamp spectra from 190 nm to 500 nm can be measured Flexible use in the entire range of HPLC Analytical flow cells and preparative flow cells Automatic recognition and storing of device specific informa tion which are important for Good Laboratory Practice oper ation qualifications or repairing the device Dual piston technology Liquid transport with stable flow rate and high
36. al several sealing disks acide hevale Fig 61 Inner components of the valve Note Pay attention to the alignment of the rotor seal in the valve during reassembly KNAUER Assistant ASM 2 1L User Manual V6815A version 1 2 70 Maintenance and Care Alignment of the Rotor Seal in the Valve Valve type Position of the rotor seal during installation Legend Multiposi Marking tion valve Cylinder pin Rotor with seal Rotor Fig 62 Position of the rotor seal in the multi position valve 2 position valve 6P 2C Fig 63 Position of the rotor seal in the 6P 2C valve 2 position valve 6P 3C Fig 64 Position of the rotor seal in the 6P 3C valve KNAUER Assistant ASM 2 1L User Manual V6815A version 1 2 71 Maintenance and Care Exchanging the VICI Valve Head Prerequisite The device has been switched off Werkzeug Tool Allen wrench size 3 mm CAUTION Damage to the valve or the valve drive Exchange the valve head with the device being switched on Loosen the screw at the valve Remove the valve Connect the new valve to the drive Tighten the screw at the valve Fig 65 Mounted VICI Valve Change settings on the Control Unit or in the software KNAUER Assistant ASM 2 1L User Manual V6815A version 1 2 72 Maintenance and Care Inserting a New Needle Seal Remove the old nee dle seal Insert the new nee dle seal into the
37. ans air will be removed from pump head and capillaries Prerequisite Capillary connections are connected KNAUER Assistant ASM 2 1L User Manual V6815A version 1 2 36 Initial Startup Tools Syringe Procedure 1 Start the pump at a low flow rate 2 Open the bleed screw of the pressure sen sor Connect the syringe with the bleed tube by using a piece of hose Suck in fluid at the bleed tube by using a syringe If the sucked fluid flows continuously stop suc tion and close the bleed screw Result No air bubbles in the pump head and in the capillaries Connecting a 2 position Valve Process Procedure Connect the capillary for sample injection to port O Connect the sample loop to the ports Connect the capillary from column to port Connect the capillary from the pump Connect tje capillary from the waste con tainer to port Fig 28 Connections for 2 posi tion valve Application Examples for Valves 2 position valve 6P 3C Injection Selection of a column Backflushing of a column Backflushing of a precolumn KNAUER Assistant ASM 2 1L User Manual V6815A version 1 2 37 Initial Startup Sample accumulation on a precolumn Alternating accumulation of a sample 2 position valve 6P 2C Preseparation and analyses on several columns after each other Sample precleaning by a precolumn Alternating precleaning of a sample Multiposition valve Al
38. boratory use EMC requirements EN 61326 1 Corrigendum 2 2011 Directives for an environmentally sound use of electrical and electronic equipment RoHS directives 2002 95 EC 2003 and 2011 65 EU on the restriction of the use of certain hazardous substances in electrical and electronic equipment WEEE directive 2002 96 EC 2003 and 2012 19 EU on waste electrical and electronic equipment The product was tested with a typical configuration Berlin 2013 06 10 Aje Dr Alexander B nz Managing Director The mark of conformity has been applied to the rear panel of the device CE Table of Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig 5 90 Table of Figures Figures ASM 2 1L in the version compact HPLC isocratic Compact HPLC HPG with UVD 2 1L 0 2 eee eee Detector in the ASM 2 1L esd ae ooee eek eee eet E Gee eee Pump with pressure transducer in the ASM 2 1L Valves in the ASM 2 1L tcces toe es eVaid oaks phan ed eiawekS 2 position valves with 6p 2C and 6P 3C 0085 Scheme of a multiposition valve 6P 0 cee eee eee 2 channel degasser in the ASM 2 1L 0 0 0 0 eee eee SmartMix WIKER lt eus sven a sisatne Se ets oe ae a Ewes eae wes AS
39. cer not part of all flow cells 3 Using the tweezers pull out the compression bushing and the light guide Assistant ASM 2 1L User Manual V6815A version 1 2 64 Result Next step Legend Capillary screw fit ting Outlet screw fitting Inlet screw fitting Fastening screw Inlet screw fitting Outlet screw fitting KNAUER 7 Maintenance and Care Carefully push the light guide out of the holder and strip off the seal The seal ring needs to be renewed during every cleaning of the optical fiber Clean the light guide with a clean soft cloth or with an appropriate solvent in an ultrasonic bath Make sure that the clean light guide is not touched by fingers Afterwards assemble the flow cell and make sure that the new seal ring does not interrupt the light path Using the wrench tighten the threaded ring The lens is clean Insert the flow cell Pump P 2 1S P 4 1S Tightening the Screw Fittings 1 Always tighten the inlet screw 1 and the outlet screw 1 with a torque wrench and 7 5 Nm Always hold the inlet fitting 1 with a wrench when the cap illary fitting is being tightened with a wrench Using an Allen wrench screw in the opposite pairs of fasten ing screws evenly and alternately to prevent the pump pis tons on the inside from jamming Loosening the Fittings Using an Allen wrench loosen the opposite pairs of fastening
40. decontaminated All materials or fluids used for decontamination must be collected separately and dispo sed of properly Flammability Organic eluents are highly flammable Since capillaries can detach from their screw fittings and allow eluent to escape it is prohibited to have any open flames near the analytical sys tem Leak sensor Observe display on the device on the control unit and in the chromatography software Eluent tray Risk of electrical shock or short circuit if liquids get into the device s interior Place all bottles in an eluent tray Eluent lines Install capillaries and hoses so as to liquids can not get into the interior in case of a leak Power strip If several devices are connected to one power strip always consider the maximum power consumption of each device Power supply cable Defective power supply cables are not to be used to connect the device and the mains power KNAUER Assistant ASM 2 1L User Manual V6815A version 1 2 20 Safety for Users Self ignition point Only use eluents that have a self ignition point higher than 150 C under normal ambient conditions Power supply Only connect devices to voltage sources whose voltage equals the device s voltage Toxicity Organic solvents are toxic above a certain concentration Ensure that work areas are always well ventilated Wear pro tective gloves and safety glasses when working on the device UV light Concentrated UV light can disperse from a d
41. diameter of 0 25 mm Connect column to Use stainless steel capillaries with an detector inner diameter of 0 25 mm Connect detector to Use stainless steel capillaries with an waste bottle inner diameter of 0 25 mm Leak Management The leak management consists of the leak sensor and the drain age system The drainage system ensures that escaping liquids flow into a waste bottle If there is to much liquid the red LED KNAUER Assistant ASM 2 1L User Manual V6815A version 1 2 47 Prerequisite Procedure Next steps KNAUER Initial Startup starts flashing Both device and data acquisition from the chro matography software are stopped Front panel has been removed Carefully push the fun nel into the center opening of the capillary N guide Push the long ending of the first nozzle into the hose Connect the nozzle and the funnel Push the other end of the hose onto the noz zle of the leak tray For the bottom device push the short end of the nozzle into the opening in the collec tion point of the leak tray Connect the hose to the nozzle and lead the second ending to the waste bottle Place the waste bottle below the bottom device a Fig 45 Funnel and capillary guide PRCI r gt PTEE Ai OOOO 7 Fig 47 Hose connected to device Fig 48 Leak tray with nozzle Reattach the front panel onto the device
42. e Note The test cell is used during operation qualifications and must be stored Prerequisite Detector has been switched off Power plug has been pulled Procedure Unscrew the 2 knurled head screws Pull out the slide as far as it takes to take out the test cell Fig 24 Removing the slide Remove the test cell Insert the flow cell into the slide Push the slide into the detector Tighten the knurled head screws Fig 25 Assembling the flow cell Result Flow cell is assembled Next steps Connecting the capillaries KNAUER Assistant ASM 2 1L User Manual V6815A version 1 2 35 Initial Startup Connecting the Capillaries CAUTION Damage to the flow cell caused by strongly tightened fittings Check the torque of screw fittings 5 Nm for stainless steel fittings 0 5 Nm for PEEK fittings Prerequisite Flow cell has been assembled Tools Open end wrench Procedure 1 Push the capillary through the fitting Push the clamping ring onto capillary Fig 26 Capillary fitting Manually screw together the capillary and the flow cell To avoid leaks tighten the fitting with a maxi mum torque of 5 Nm by using the open end wrench Fig 27 Capillary and flow cell Result Capillaries have been connected and the detector is ready for operation Bleeding the Pump Before the pump can be used for analyses it has to be bleeded That me
43. e Pump heads 0 5 mm 1 16 0 8 mm 3 ul Stainless steel 250 ml min 200 bar 0 5 mm 1 16 0 8 mm 3 ul PEEK 250 ml min 100 bar 8 mm 1 16 0 15 mm 0 14 ul Quartz glass 0 10 ml min 500 bar 8 mm 280 um 0 015 mm 0 035 ul Quartz glass 0 01 ml min 500 bar 10 ml pump head stainless steel 10 ml pump head titanium 10 ml pump head ceramics 50 ml pump head stainless steel 50 ml pump head titanium Assistant ASM 2 1L User Manual V6815A version 1 2 82 Spare Parts and Accessories Valves Injection syringe 100 ul 2 position valves 6P 3C 1 16 stainless steel 6P 2C 1 16 stainless steel ee a 6P 3C 1 16 PEEK A1358 6P 2C 1 16 PEEK A1358 1 6P 3C 1 8 stainless steel luer lock can A1359 nula 6P 3C 1 8 PEEK luer lock cannula A1360 6P 3C 1 16 stainless steel A1369 6P 2C 1 16 stainless steel A1369 1 6P 3C 1 16 PEEK A1370 6P 3C 1 8 stainless steel luer lock can A1371 nula 6P 2C 1 8 stainless steel luer lock can A1371 1 nula KNAUER Assistant ASM 2 1L User Manual V681 5A version 1 2 83 Spare Parts and Accessories Sample Loops for the Valve Versions of the listed sample loops with 1 16 and in stainless steel Note Sample loops in PEEK are also available Sample loops in PEEK and stainless steel are also available with fittings 1 8 Holding Bracket for Valves Columns and Flow Cells
44. e car tridge KNAUER Assistant ASM 2 1L User Manual V6815A version 1 2 75 KNAUER Maintenance and Care Check the PEEK sealing ring for any possible damage replace sealing ring if necessary filter insert kit AO164 1 2 Take cartridge out of the housing Insert the new cartridge The insertion direction does not play any role hereby Do not remove the filters in the lid Place the PEEK sealing ring on the cartridge Put on the mixer lid with the screw fittings of the capillaries as shown in the picture Put the mixer lid onto the mixer housing with the cartridge and the PEEK sealing ring Tighten the four screws on the mixer lid with an Allen wrench wrench size 3 0 x 150 Assistant ASM 2 1L User Manual V6815A version 1 2 76 Technical Data Technical Data Ambient Conditions Air humidity 10 90 non condensing Assistant ASM 2 1L Maximum weight approx 14 kg depending on con figuration Dimensions 361 x 158 x 523 mm Width x Height x Depth Power supply 100 240 V 50 60 Hz maxi mum 100 W Operation Digital via LAN Control Unit Analog Integrator 2 5 V Pin header Degasser DG 2 15S Function Gas permeation through a fluoro polymer membrane Inner diameter of the 1 14 mm Teflon AF capillaries approx 285 ul per channel Wetted materials PEEK glass filled PTFE Teflon AF KNAUER Assistant ASM 2 1L User Manual V6815A version 1 2
45. e hazard Leak hazard Pull the power plug Wear protective gloves Observe torques KNAUER Assistant ASM 2 1L User Manual V6815A version 1 2 22 Unpacking and Setup Unpacking and Setup Phone Fax E mail Power supply Requirements KNAUER Contacting the Technical Support You have various options to contact the technical support 49 30 809727 111 49 30 8015010 support knauer net You can make your requests in English and German Location Requirements CAUTION Possible damage to the device due to overheating Set up the device so that it is pro tected against exposure to direct sunlight Make sure the room is well ventilated Allow 30 cm space at the rear of the device for air circulation The device is only suitable for alternating current The supplied power cable is to be used to connect the device to the mains supply The location for the device must meet the following require ments Maximum weight about 14 kg Dimensions 361 mm x 158 2 mm x 523 mm Width x Height x Depth Power supply 100 240V 50 60 Hz maximum 100 W Air humidity 10 90 non condensing Temperature 10 40 C 39 2 104 F Space require Laboratory table for safe and com ments fortable working Ventilation and cabling At least 30 cm clearance around the system 1 Depending on the configuration of the device Assistant ASM 2 1L User Manual V6815A version 1 2 23 Tools Pr
46. e motor Replace the motor Pump min motor Replace the motor current detected Check the motor contacts Pump min pres Localize leak in the system and sure detected remove it Vv Valves have differ Correct the settings Both valves have ent types in column to be of the same type switch mode Valve left failure Left valve is stiff detected Drive is defective Valve middle failure Middle valve is stiff detected Drive is defective Valve right failure Right valve is stiff detected Drive is defective W Wrong valve type Correct the settings KNAUER Assistant ASM 2 1L User Manual V681 5A version 1 2 60 Maintenance and Care Maintenance and Care Serial number Year of manufacture KNAUER Proper maintenance of your HPLC device will ensure successful analyses and reproducible results Maintenance Contract The following maintenance work on the device may only be per formed by KNAUER or a company authorized by KNAUER and is covered by a separate maintenance contract Opening the device Removing the hood or the side panels Required System Information The serial number can be founded with the Control Unit Menu gt GLP as well as on the rear side of the device The year of manufacture forms part of the serial number of the module example CDA103252525 The letters designate the device type the year of manufacture is stated as year and calen dar week In the example
47. e of charge This warranty excludes the following 1 Accidental or willful damage 2 Damage or errors caused by third parties that are not con tractually related to the manufacturer at the time the damage occurs 3 Wear parts fuses glass parts columns light sources cuvettes and other optical components 4 Damage caused by negligence or improper operation of the device and damage caused by clogged capillaries 5 Packaging and transport damage In the event of device malfunctions directly contact the manu facturer Manufacturer Wissenschaftliche Ger tebau Dr Ing Herbert KNAUER GmbH Hegauer Weg 38 14163 Berlin Germany Phone 49 30 809727 0 Fax 49 30 8015010 E Mail info knauer net Internet www knauer net Transportation Damages The packaging of our devices provides the best possible protec tion against transportation damage Check the devices for signs of transportation damages In case you notice any damage con tact the technical support and the forwarder company within three workdays KNAUER Assistant ASM 2 1L User Manual V6815A version 1 2 88 Disposal AVV marking WEEE registration KNAUER Disposal Drop off old devices at the certified waste facilities where they will be disposed of properly According to the German Abfallverzeichnisverordnung AVV January 2001 old devices manufactred by KNAUER are marked as waste electrical and electronic equipment 160214 KNAU
48. ector calibra Check lamp tion failed Check piezomotor with filter wheel Detector D2 lamp Change the D2 lamp failed Detector D2 lamp Install a D2 lamp not detected Detector external Error IN is connected to another error detected device which is defective Detector grating Replace step motor of the grating drive failed drive Replace the mainboard Detector low light Check lamp Clean flow cell Detector not cali Activate calibration brated Detector validation Install test cell and activate calibration failed E Error input activated Device error Change the device set tings G GUI communication Replace the display failed L Leak sensor not pres Switch the device off and then on ent If the leak sensor is still not present contact the Technical Support of KNAUER M Maximum pressure Reduce the pressure or adjust the System stopped upper pressure limit Restart the system Minimum pressure Increase the pressure or adjust the System stopped lower pressure limit Restart the system KNAUER Assistant ASM 2 1L User Manual V6815A version 1 2 59 Troubleshooting P Pump external error Localize the external error and detected remove it Replace the motor Replace the mainboard Pump max current Replace the motor detected Replace the mainboard Pump max pres System pressure is too high sure detected Remove the blockage Check the contacts at th
49. en For the 10 ml pump head ceramic can be chosen Pump P 4 1S Pump with pressure transducer with a 10 ml or 50 ml pump head As a mate rial for the pump head interior tita nium or stainless steel can be chosen For the 10 ml pump head ceramic can be chosen Detector UVD 2 15S UV Vis detector with variable wave length 190 nm 500 nm Valve drive V 2 15S Valve drives 6V 12V 16V for manual valves Electrical valves Valves of the company VICI Valco Instruments Co lnc Assistant ASM 2 1L User Manual V681 5A version 1 2 12 Legend Detector Flow cells Analytical flow cells Preparative flow cells KNAUER Device Overview Device Comment KNAUER 2 position valves or multi position valves Manual valves Valves of the company VICI Valco Instruments Co Inc Degasser DG 2 15S By using a degasser a more stable baseline can be obtained Detector in the ASM 2 1L The ASM 2 1L can be equipped with a detector O Fig 3 Detector in the ASM 2 1L The UVD 2 185 will be delivered with a test cell For the use of the detector a flow cell has to be installed Flow cells are available with different layer thicknesses connec tions and also for different maximum flow rates The flow cells are made of stainless steel or PEEK U Z View micro flow cells are made of quartz glass Layer thick Connec ness tors 1 16 Stainless 20 50 ml min steel Layer thick ness 3 mm also in PEEK
50. er Manual V681 5A version 1 2 27 Initial Startup Initial Startup Holding Bracket A holding bracket can be mounted laterally to which valves col umns or flow cells are attached Legend Holding bracket with valve Fig 12 Valve installed laterally on the device Following accessories can be mounted onto the side panel Accessories Comment Valves KNAUER valve Mounting directly at the holding bracket VICI Valco valve Mounting directly at the holding bracket VICI Valco valve drive Mounting by using the adapter plate at the holding bracket Flow cells KNAUER flow cells Mounting by using the adapter plate at the holding bracket Columns Axial compressible col Mounting by using the adapter umns Vertex Plus AX plate at the holding bracket 20 mm and 30 mm Sample loop Preparative sample loops Mounting by using the adapter 11 ml and 40 ml plate at the holding bracket KNAUER Assistant ASM 2 1L User Manual V681 5A version 1 2 28 Initial Startup Attaching the Holding Bracket to the Side Panel Before accessories can be mounted the holding bracket has to be attached to the side panel Procedure Attach the holding bracket with two Allen screws by using a M3 x 6 wrench Tools Allen wrench size 2 5 Procedure 1 Position the holding bracket onto the bore holes at the side panel 2 Using the wrench fasten the screws Fig 13 Bore holes to attach hold ing bracket on the side pa
51. etector without flow cell or from the optical fiber which leads to irritation of the retina When the flow cell or the optical fiber is exchan ged switch the device off and pull the power plug Where is use of the Never use the system in potentially explosive atmospheres device prohibited without appropriate protective equipment For further informa tion contact the technical support of the manufacturer Opening the device The device may only be opened by the technical support of the manufacturer Definition of Personal and Material Damages Possible dangers related to the device are distinguished in perso nal and material damages in this user manual Category Explanations DANGER Lethal or very serious injuries can occur CAUTION Moderate injuries or device defects can occur KNAUER Assistant ASM 2 1L User Manual V6815A version 1 2 21 Symbols and Signs Symbols and Signs The following table explains symbols and labels which are used on the device on the touchscreen of the Control Unit in the software or in the manual Warning signs Mandatory signs Device fulfills the requirements of the Con formit Europ enne which is confirmed by the Declaration of Conformity Damage to the leak tray or front cover pos sible while carrying setting up and install ing a device Grip the device at its sides near the middle when lifting or moving High voltage hazard UV light hazard Electrostatic discharg
52. ge Analytical cartridge 350 ul mixture volume Micro cartridge 100 ul mixture volume Assistant ASM 2 1L User Manual V6815A version 1 2 Compact HPLC HPG 79 Spare Parts and Accessories Device Spare Parts and Accessories Note If a compact HPLC was ordered tools and capillaries are within the scope of delivery The torque wrench for pumps is not within the scope of delivery fame derma ASM 2 1L with micro devices depending on selection User manual German V6815 User manual English V6815A Accessories kit AZURA FZA02 Accessories Compact HPLC Capillary kit 1 16 stainless steel A9849 UVD 2 1L EDAO1 Flow cell A4061 Magnetic column holder A9847 Flow Cells Layer thickness Connection Inner diameter Volume Material Analytical Max flow rate Max pressure Analytical Layer thickness Connection Inner diameter Volume Material Max flow rate Max pressure KNAUER 10 mm A4061V2 1 16 1 1 mm 10 ul Stainless steel with heat exchanger 20 ml min 300 bar 10 mm 1 16 1 1 mm 10 ul Stainless steel with heat exchanger 20 ml min 300 bar Assistant ASM 2 1L User Manual V6815A version 1 2 80 Spare Parts and Accessories Analytical Analytical Analytical Preparative Preparative Preparative KNAUER Technical Data Path length Connection Volume Inner diameter Max flow rate Max pressure Body material Layer thickness Connectio
53. ion as a compact HPLC a valve is mounted laterallly on the ASM 2 1L An eluent tray will be supplied to the compact HPLC Fig 1 ASM 2 1L in the version compact HPLC isocratic Compact HPLC for High Pressure Gradients For a high gradient HPLC system the ASM 2 1L needs to be combined with a detector UVD 2 1L The ASM 2 1L is equipped with following devices Pump 2 1S without pressure transducer with 10 ml pump head Pump 4 1S with pressure transducer with 10 ml pump head Degasser DG 2 1S analytical 2 channel Smartmix 350 A valve is mounted laterallly on the detector UVD 2 1L An elu ent tray is supplied with the compact HPLC system Assistant ASM 2 1L User Manual V681 5A version 1 2 11 Legend Solvent tray Detector Pump with Smart mix Pump with pressure transducer Degasser Valve Devices in the ASM 2 1L KNAUER Device Overview More information about the detector UVD 2 1L can be found in the corresponding user manual Fig 2 Compact HPLC HPG with UVD 2 1L Device Overview in the ASM 2 1L As an additional module for the HPLC system the ASM 2 1L can be equipped with different devices on request Following devices can be mounted in the ASM 2 1L Device Comment Pump P 2 1S Pump without pressure transducer with a 10 ml or 50 ml pump head As a material for the pump head interior titanium or stainless steel can be cho s
54. is connected to the workstation and router The chromatography software has been installed by KNAUER or a company authorized by KNAUER The capillaries in the solvent bottles have a filter insert All capillaries are tightly connected Assistant ASM 2 1L User Manual V6815A version 1 2 51 Initial Startup Startup of the Device CAUTION Possible damage to the device caused by condensed water Allow device to acclimate for 3 h before it is taken into operation Prerequisite Capillaries tubings and cables are connected In case of the detector the flow cell is installed Liquid container is sufficiently filled Washing container is sufficiently filled CAUTION Damage to the pump head in case it runs dry Ensure that liquids runs through pump head and piston backflushing Process Procedure Switch on the device at the power switch on the rear side 2 Wait until the device has completed the self test If the self test has been successfully com E pleted the LED on the right lights up green Fig 50 Power switch on the rear side of the device Note The firmware is successfully initialized if shortly after switching on the device all three LEDs light up red green and blue for approx 1 second Result The device is ready for operation If the test fails an error mes sage will be displayed Contact the technical support of KNAUER if the error occurs several times Practical Tip Regu
55. is mounted KNAUER Assistant ASM 2 1L User Manual V681 5A version 1 2 33 Note Prerequisite Tools Legend Threaded ring Cover Spacer Compression bush ing Light guide with PTFE seal Procedure KNAUER Initial Startup Detector Before the detector is ready to use a flow cell must be installed Before installing the flow cell the optical path length can be changed The optimal path length depends on type and quan tity of the sample Setting the Optical Path Length WARNING Irritation of retina through UV light Concentrated UV light can leak out from the flow cell or the fiber optic connectors Switch off the device and pull the power plug The optical path length is set to 2 mm at the factory You can remove the spacers on one or both sides and hence change the path length to 1 25 mm or 0 5 mm Flow cell has been disassembled 3 mm Allen wrench Fig 23 Preparative flow cell Using the wrench unscrew and remove the threaded ring Remove the cover and spacer Put the spacer aside 1 2 3 Insert the cover 4 Using the wrench tighten the threaded ring Assistant ASM 2 1L User Manual V681 5A version 1 2 34 Initial Startup Enlarging the optical To enlarge the optical path length put in one or both spacers path length Inserting the Flow Cell The test cell has no connectors for capillaries and must therefore be replaced by a flow cell before first usag
56. ix The pump was Exchange the filter system mixer switched off auto matically because Pmax was exceeded Pressure increase Replace cartridge Filter system inserted Exchange the filter system wrong Further measures Inform the Technical Support of KNAUER System Messages If other system messages are displayed besides those listed below please turn the device off and then on Inform the Tech nical Support of KNAUER in case the system message repeats itself The system messages are sorted alphabetically A At least one wave Check whether a channel is on Check length must be whether the wavelengths are within valid permissible range 190 to 900 nm C Calibration failed Switch the device off and on Check whether lamps motor and filter are functioning correctly Inform the Technical Support of KNAUER in case the system message repeats itself Restart calibration on the device or in the chromatography software Cannot initialize Check cables and connections in local area network Cannot proceed D2 Wait for the ignition of the D2 lamp lamp heating Cannot proceed D2 Switch D2 lamp on before RUN lamp is OFF KNAUER Assistant ASM 2 1L User Manual V6815A version 1 2 58 Troubleshooting Cannot proceed Switch the lamps on the device to Lamps are off ON Cannot read RTC Switch the device off and on Inform the Technical Support of KNAUER in case the system message repeats itself D Det
57. larly check the capillaries for leaks KNAUER Assistant ASM 2 1L User Manual V6815A version 1 2 52 Operation with Chromatography Software or Control Unit Operation with Chromatography Software or Control Unit Legend Left LED Center LED Right LED Switch AZURA Standby KNAUER A device can be operated in two ways Control with chromatography software Control with Control Unit Control with Chromatography Software To control the device with chromatography software it must be connected to the computer through the LAN interface Devices can be controlled with e g OpenLAB EZChrom edition version A 04 05 or higher ChromGate version 3 3 2 or higher and ClarityChrom version 3 0 7 or higher You will find a detailed description on the chromatography soft ware in the software manual Control with Control Unit You can control the device using the touchscreen on the mobile Control Unit The Control Unit is an optional accessory You will find a detailed description on the Control Unit in its accompany ing user manual Meaning of the LEDs There are three LEDs and a switch on the front of the device Fig 51 LEDs and switch on the front of the device The LEDs can have different colors depending on the operating conditions To activate the standby keep the switch pressed for 5 seconds Assistant ASM 2 1L User Manual V6815A version 1 2 53 Operation with
58. n Inner diameter Volume Material Max flow rate Max pressure Layer thickness Connection Inner diameter Volume Material Max flow rate Max pressure Layer thickness Connection Inner diameter Volume Material Max flow rate Max pressure Layer thickness Connection Inner diameter Volume Material Max flow rate Max pressure Layer thickness Connection Inner diameter Volume Material Max flow rate Max pressure 10 mm 1 16 10 ul 1 1m 20 ml min 300 bar Stainless steel with heat exchanger 3mm 1 16 1 0 mm 2 ul Stainless steel 50 ml min 300 bar 3mm 1 16 1 0 mm 2 ul PEEK 50 ml min 30 bar 0 5 1 25 2 mm 1 8 2 3mm 25 ul Stainless steel 1000 ml min 200 bar 0 5 1 25 2 mm 1 8 2 3mm 25 ul PEEK 1000 ml min 100 bar 0 5 1 25 2 mm 1 4 4 0 mm 160 ul Stainless steel 10000 ml min 200 bar Assistant ASM 2 1L User Manual V6815A version 1 2 81 Spare Parts and Accessories Preparative Preparative U Z View Micro U Z View Micro KNAUER Technical Data Layer thickness Connection Inner diameter Volume Material Max flow rate Max pressure Layer thickness Connection Inner diameter Volume Material Max flow rate Max pressure Layer thickness Connection Inner diameter Volume Material Max flow rate Max pressure Layer thickness Connection Inner diameter Volume Material Max flow rate Max pressur
59. n B 2 0 00 Backflushing Transport of the sample onto the column Backflushing of the column 0 eee ee eee eee Use of pre and main column 0 ce eee eee eee eee Backflushing of the precolumn 0 0 eee eee eee Column selection with a 6P 2C valve 00 02 ee eee Precleaning of a sample by a precolumn 04 Front view degasser i 4 dessvie s was aN id WS REGS Aa ene Re ged Fittings solvent hose sca 043 2 55 oad cibsady tobe sikrest ASM 2 1L gas outlet on the front 2 eee ee eee KNAUER Assistant ASM 2 1L User Manual V6815A version 1 2 Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 91 Table of Figures Scheme of an isocratic compact HPLC system 45 Scheme of a high pressure gradient compact HPLC system 46 Funnel and capillary guide i 20 00 se4 nok ea etee ey eae eee 47 HOs and nozzle gre wees dod eee SoMa Wes Ge das 47 Hose connected to device 6 eee eee eee 47 Leak tray with nozzle i oc nunun ease dae EAN ag ee ales 47 Cabling system LAN so cetie 22 28a a Boeke dae se eee RS 49 Power switch on the rear side of the device 52 LEDs
60. nauer Devices Objective The objective of the installation qualification is to define requirements and acceptance criteria for the device Proper delivery and installation of the device will be documented Instructions The IQ is carried out by an authorized Knauer agent in accordance with the instructions described below as well as the attached and approved report Any deviations to the specifications and problems encountered during installation shall be recorded as comments at the end in the document Installation Qualification Deviations In addition all courses of action undertaken to resolve the problems and remove the deviations shall be recorded as comments in the document Installation Qualification Deviations If certain items in the report are not applicable these must be indicated by n a not applicable Larger sections that are not used must be crossed out diagonal line through them marked with n a and date DD MM YYYY and signed Enter yes no or n a in the column Within the scope of delivery in the tables In the Comments column enter the reference number for the document Installation Qualification Deviations The deviation is described with the corresponding reference number in the document Installation Qualification Deviations All required documents should be completed promptly on site The document will be reviewed by a person authorized by
61. nel Fig 14 Holding bracket is attached Result Holding bracket is attached Next steps Mount accessories like valves flow cells or columns to the hold ing bracket KNAUER Assistant ASM 2 1L User Manual V681 5A version 1 2 29 Initial Startup Mounting a KNAUER Valve Prerequisite Holding bracket is attached to the side panel Tools Allen wrench size 3 Process Procedure 1 Position the valve to the front side of the holding bracket Fasten the valve with two screws and two washers from the back side Fig 15 Front side of KNAUER valve at the holding bracket Fig 16 Back side of KNAUER valve at the holding bracket Result The KNAUER valve is mounted KNAUER Assistant ASM 2 1L User Manual V6815A version 1 2 30 Initial Startup Mounting a VICI Valco Valve Prerequisite Holding bracket is attached to the side panel Tools Allen wrench size 2 5 Process Procedure 1 Position the adapter plate to the back side of the holding bracket Slide the valve through the hole of the holding bracket and the adapter plate forwards as far as possible Fasten the valve with four screws to the front side Back side of VICI Valco valve at the holding bracket Fig 18 Back side of VICI Valco valve at the holding bracket Result The VICI Valco valve is mounted KNAUER Assistant ASM 2 1L User Manual V6815A version 1 2 31 Initial Startup Mounting a VICI Valco valve dri
62. network do not overlap 2 In case of an overlapping change the IP address range of the router 3 Use the patch cable to connect the router WAN port to the company network 4 Restart all device including the computer Controlling Several Systems Separately in a LAN Devices connected to a LAN communicate through ports which are part of the IP address If more than one HPLC system is con nected to the same LAN and you plan on controlling them sepa rately you can use different ports to avoid interference Therefore the port number for each device must be changed and this same number must be entered into the device configu ration of the chromatography software We recommend to use the same port number for all devices in the same system The port ist set to 10001 at the factory The IP port numbers in the device configuration of the chromatography software must be identical to those in the device otherwise the connection fails 1 Change the port number of the device 2 Enter the port number in the chromatography software The connection is established Refer to the troubleshooting chapter if you experience connec tion problems Checklist prior to switch on Use this checklist to determine whether the system is ready for initial startup The modules are at the desired location The power plugs of the modules are plugged in The LAN connections between the modules and router are connected The LAN cable
63. ni sanaa aN AANS Aaa a ARARE 2 IQ certificate a anana ens eee a E EE ee ert 3 OI aaa sa reia dota mec ei teeeneteeerecieees 4 Shipkit list of hardware and software c0ccceecseeeeeeeeeseeeeeeseeesaneeenseeeseneeenseeesaees 5 Initial StartUp cnnan AEiG A AEAEE orca 6 Installation Qualification Deviations ccccccccceeeeseeeeeeeeeceeeeeeseeesaeeeesenennenensaes 7 App ndiceS wa ceriiacesauitavacsnitadssmnwsucavvcanaunvewsnaustenbuexuyicatauatenteawaveaunee heanavavenalanmesiouans 8 COmMMENUS aeiiae nea eG er E A EAE 9 Rules on reproduction and duplication This document contains confidential information and may not be reproduced without the written consent of Wissenschaftlicher Ger tebau Dr Ing H Knauer GmbH KNAUER 13 08 2012 ASM 2 1L Rev1 IQ2 9 HPLC SMB Osmometry Ih KNALER IQ for Knauer Devices IQ certificate The authorized Knauer agent will inspect the device and perform the necessary tests as described in the IQ document All results are noted using a blue pen and confirmed with the date DD MM YYYY and signature The IQ has been performed to the satisfaction of both parties The users listed below were instructed and are familiar with how to operate the device Responsible person Name in block letters Date Signature Authorized agent of customer Name in block letters Date Signature KNAUER 13 08 2012 ASM 2 1L Rev IQ3 9 HPLC SMB Osmometry Ih KNALER IQ for K
64. nt ASM 2 1L User Manual V6815A version 1 2 54 Installation Qualification IQ Installation Qualification IQ Installation report Certification on the functionality of the device During installa tion of the device an installation report Q document is cre ated upon request in coordination with the Technical Support of KNAUER This installation report needs to be completed in full and signed by both parties It serves as proof of the properly executed installation and functionality of the device Operation Qualification OQ Extensive Extensive test of the detector s functionality A successfully exe functionality test cuted OQ ensures that the detector functions properly Test Intervals Run the extensive functionality test at the following time inter vals 1 to 5 days week Every 6 months More than 5 days week or 24 hours day Every 3 months Operation with buffer solutions or other salt Every 3 solutions months Execution The test can carried out either by KNAUER s Technical Support or by a technical service authorized by KNAUER The OQ documentation required for executing the OQ is with costs once and can be ordered separately from KNAUER KNAUER Assistant ASM 2 1L User Manual V6815A version 1 2 55 Troubleshooting Troubleshooting First measures 1 Check all cabling 2 Check all screw fittings 3 Check whether air has gotten into the supply lines 4 Check device for leaks 5 Pay at
65. ocedure Note KNAUER Unpacking and Setup Unpacking CAUTION Damage to the flow cell caused by carrying the detector incorrectly Lift the detector up with your hands grasping the side panels CAUTION Damage to the leak tray or front cover possible while carrying setting up and installing a device Grip the device around the middle of its side panels In case of the preparative pump ask a second person for help Store all packing materials Included packing list should be kept for repeat orders Utility knife 1 N 8 9 Check for damages caused during transportation In case you notice any damage contact the technical support and the forwarder company Setup the delivery so the label is in the correct position Using the utility knife cut the adhesive tape Open the delivery Remove the foam insert Take out the accessories kit and the manual Open the accessories kit and take out all accessories Check the scope of delivery In case any parts are missing contact the technical support Grip the device at its side panels and lift it out of the packag ing Remove the foam inserts from the device Check for damages caused during transportation In case you notice any damage contact the technical support Set up the device in its location Remove the protective foil The leak sensor may malfunction if the device stands on a tilted surface Use a level to check that
66. paration of sample mixtures using high pressure gradient of pump HPLC High performance liquid chromatography Integration The integration time determines how fast the time detector reacts to changes in absorbance Isocratic Sample mixtures separated with a constant composition of solvent The IQ is the certification on the functionality of the device Lightemitting diode Luer lock Standardized connection system between cannula syringes and cannulaes Comprehensive functionality test of individual components in a system operation qualifica g me GLP Good Laboratory Practice quality assurance for laboratories PG tion Retarding A substance which has a delayed elution caused by an interaction with the column fill material Assistant ASM 2 1L User Manual V6815A version 1 2 86 Abbreviations and Terminology Router Module in computer network that checks data packets and forwards them Solvents Mobile phase eluent or carrier for liquid chro matography KNAUER Assistant ASM 2 1L User Manual V6815A version 1 2 87 Legal Information Legal Information Warranty Conditions The manufacturer s warranty for the device is valid for 12 months after the date of dispatch All warranty claims shall expire in the event that any unauthorized changes are made to the device During the warranty period any components with material or design related defects will be replaced or repaired by the manu facturer fre
67. plied within the scope of delivery STR PRT PR Pe Internet Fig 49 Cabling system LAN Computer is on There is a patch cable for each device and the computer 1 Use the patch cable to connect the router and the computer Repeat this step to connect all devices 2 Use the power supply to connect the router to the mains power system Configuring the Router The router is preset at the factory You can find a label at the bottom side of the router on which IP address user name and passwort are printed These information help to open the router configuration 1 To open the router configuration start your Internet browser and enter the IP address not for all routers 2 Enter user name and password Configure the router as DHCP server A w In the router configuration check the IP address range and make changes if necessary Once the router has assigned IP addresses to all devices the chromatography software can be used to remotely control the system Assistant ASM 2 1L User Manual V681 5A version 1 2 50 Prerequisite Procedure Note Procedure Result Note KNAUER Initial Startup Integrating the LAN into a Company Network A network administrator can integrate the LAN into a company network In this case the WAN port of the router is used There is a patch cable for the connection 1 Check that the IP address range of the router and of the com pany
68. rench 4 Connect the eluent line to the pump head Exchanging the Check Valves Note The check and position of the valves have been harmonized to each other Insert the valves in the direction of flow Legend Check valve Check dotted line Flow direction arrow Fig 56 Functional principle of the check valve Dirty check valves do not open and close properly They cause pressure fluctuations and irregular flow Purge the pump head before changing the check valve Removing the Check Valves Note Loosen the screw fittings of the capillary connections alternately to prevent the capillaries from bending Unscrew the inlet fitting 2 and capillary fitting Unscrew the outlet fitting 1 1 2 3 Remove the check valve 4 Unscrew the inlet fitting 1 5 Remove the check valve KNAUER Assistant ASM 2 1L User Manual V681 5A version 1 2 67 Maintenance and Care Legend Inlet screw fitting 2 Capillary screw fit ting Outlet screw fitting 1 Check valve Inlet screw fitting 1 Fig 57 Exchange the check valves Cleaning the Check Valves 1 Put the valve in a beaker with rinsing solution for example isopropanol or methanol 2 Put the beaker with valve in an ultrasonic bath for at least 10 minutes Installing the Check Valves 1 Insert both check valves 2 Screw in the outlet fitting 1 and tighten to 7 5 Nm with a torque wrench 3 Screw in
69. ssories 18 Additives 8 Ambient conditions 77 Analog port 24 Application examples 36 AVV marking 83 B Backflushing Column 39 Precolumn 40 Ball valve Cleaning 68 Installing 68 Removing 67 Replacing 67 C Capillary Clamping ring 35 Fitting 35 Torque 35 Care 61 Cartridge Replacing 75 CE marking 84 Column Backflushing 39 Selection 38 41 Connectors 23 Control Control Unit 53 54 Chromatography Software 53 54 D Declarationf of conformity 84 Decontamination 19 Degasser 42 Detector Rear panel 24 44 Disposal 83 E Eluent Flushing solution 62 Eluents 8 Flammability 19 Self ignition point 20 Toxicity 20 Environmental protection 83 F Fitting Screwing tight 65 Flow cell 12 Cleaning 62 Flushing 62 Installation 34 Lense 63 Light guide 64 Micro flow cells 12 13 Optical wavelength 33 G GROUND see spring strip 26 Installation Qualification 55 Funktionstest 89 IQ 55 L LAN Port 50 Router 49 Settings 48 Setup 48 Troubleshooting 50 Leak management 46 Location 22 M Maintenance 61 Maintenance contract 61 Maintenance by users 61 Mandatory signs 21 Manufacturer 82 Modifiers 8 N Neelde seal Installing 73 KNAUER Assistant ASM 2 1L User Manual V681 5A version 1 2 93 Index O Operation Qualification 55 OQ 55 P Power supply 20 22 Cable 19 Power strip 19 Precolumn backflushing 40 Pump head Dismounting 66 Mounting 67 Replacing 66 R
70. t of the Rotor Seal in the Valve 00 0 0 70 Exchanging the VICI Valve Head 2 eee ee eee 71 Inserting a New Needle Seal 2 0 0 0 cece ee eee eee eee 72 DEQASSER vatecteh deaelats sa a a diate ok alah ates ha 72 SmartMix Mixer in the ASM 20 o6 3 520 pbs ong neo Fe aes ose wow ewes 72 Opening the SmartMix Mixer 45 ous ee a ee elle Se oe ARO ee aes 73 Exchanging the Filter System oss 4scscuced es baad cee ee se ade ead 73 Replacing the Catnidqe ca saind cance GORE DR ORS SLE RRR EN Ses 74 Technical D ta aoc ese lans feet tee he ieee whee ees eA eee 76 Ambient Conditions sserrs sierosa dane eh dated eee wns dad 76 Assistant ASM 2olL 2 24 250 084 gan teae Gas oe tea See ee ewe eae eee ts 76 Degasser DG 2 15 2 hake eed se oes ial Pee RES ee Nee PONTE RO ae eS 76 Detector UVD 2 15 vied it eh eitee tal Gatti alta ea RE Gis bps boot te sath 2 77 P mp P25 P AAS cst teagan iad tu a bate Wal eae rma OAT 77 WAIVE Drive V 2 TS retiaria oon eee ive tek du towe ws Cia e tla 78 VAIS aisead a nA Aa os Ses BEN RS Shae a ay Relea men eh ax doe 78 SmartMix Mix r pomoiid gn de aioe ae ee aes a a eee eG ae ea aes 78 KNAUER Assistant ASM 2 1L User Manual V6815A version 1 2 Spare Parts and Accessories 5 2 0 or os eatnw di eins oe aes bBo SRN 79 DEVICE sess Sea hee ea Aes Gye oe hie hy Baha Se BED este aetna Ret ee Beni Sao 79 FIG WE Cells turrets area ten at esas Ween rota a rh ilk ae aie Mela Rt Ria ae a arn 79
71. tention to system messages Further measures Inform the Technical Support of KNAUER Possible Problems and Rectifications Problem Solon Assistant Device will not turn Inspect the power cable to ensure that on it is plugged into the power supply Detector Baseline drift Maintain constant temperature conditions during the measure ment Avoid air draft Avoid vibrations Device cannot be Fasten the knurled head screws on calibrated the flow cell to prevent incursion from interfering light or an elec tronics error Insert the test cell Inspect the calibration with a weak absorbing eluent Baseline noise Inspect the flow cell assembly Fasten the knurled head screws on the flow cell to prevent incursion from interfering light or an elec tronics error Exchange the defective flow cell Inspect the service life of the lamp The relationship of Flush the flow cell the signal to the 2 Clean the flow cell window light path reference is very low Pump When purging the Check if the bleed screw on the pres pump switches off sure sensor is turned open Replace the lamps KNAUER Assistant ASM 2 1L User Manual V6815A version 1 2 56 Troubleshooting Pump does not Purge the pump head to remove transport solvent the air bubbles Inspect the eluent filter of the HPLC column and change when blocked Clean checkl valves Exchange the check valves When the pump head seals are defective
72. ternating switching of up to 16 columns Selection of an eluent Sample fractioning Fig 29 Valves in the ASM 2 1L 2 position valve 6P 3C Valve position In the valve position Load the sample is filled into the sample Load L loop Valve position In the valve position Inject the sample is transported to the col Injecting I umn for separation Port 1 Sample injection Port 2 Sample loop Port 3 Column Port 4 Pump Port 5 Sample loop Port 6 Abfall Fig 30 Valve position Load Fig 31 Valve position Inject KNAUER Assistant ASM 2 1L User Manual V6815A version 1 2 38 Initial Startup The sample loop is filled by injecting the sample at port 1 The sample runs into the sample loop port 2 and 5 and excessive sample is transported into the waste container through port 6 The valve will be switched from Load to Inject The pump port 4 transports the sample to the column port 3 The eluent flows through the sample loop in opposite direc tion during the injecting see arrows in the figures Reed contact The reed contact informs the analytical system whether the valve is positioned to LOAD or INJECT Column selection Basics The column selection is used in chromatography for example in the following cases Method development Usage of different columns in the same HPLC system From the valve the sample is transported through the channel 2 gt 1onto the column A From connec
73. the inlet fitting 1 and tighten to 7 5 Nm with a torque wrench 4 Screw in the inlet fitting 2 and capillary fitting and tighten with an Allen wrench Valve Removing the Valve During assembly make sure the rotor seal is in the right position and installed properly because otherwise there may be a mix up of the channels or clogging may occur KNAUER Assistant ASM 2 1L User Manual V6815A version 1 2 68 Maintenance and Care Note During disassembly make sure to mark the position of the rotor seal on the cylinder pins to make the assembly of the valve eas ier 1 Loosen the three screws alternat ingly with a screw driver M3 Hold the housing of valve together when the screws are removed so that the individ ual parts do not fall Open the valve housing out Take off the top part The cylinder pins the inner compo nents and the rotor seal become visi ble Remove the top part of the valve Remove the rotor seal and deposit true sided Mark the position and the installation position of the rotor seal Deposit the rotor seal true sided with the marking of the position KNAUER Assistant ASM 2 1L User Manual V6815A version 1 2 69 Maintenance and Care 4 Hold the inner com ponents of the valve and turn over the bottom part in order to deposit the inner parts orderly Rotor plate with cyl inder pins four disk springs in two groups of two rotor se
74. tion 5 the components of the sample are forwarded separately to the detector Legend Detector Column A Valve Column B Fig 32 Column selection Usage of Column A Note Make sure there is no pressure when switching so that the col umns are not damaged KNAUER Assistant ASM 2 1L User Manual V6815A version 1 2 39 Legend Detector Column A Injection Column B Basics Note Legend Detector Valve Column Note KNAUER Initial Startup After switching the valve the sample is transported through the channel 2 gt 3 to the column B From connection 5 the compo nents of the sample are forwarded separately to the detector Fig 33 Column selection Usage of Column B Backflushing of a Column The backflushing of a column is used in chromatography for example in the following cases Analysis of heavily retarding substances Optimization of analysis times Flushing of a column The eluent flushes the column after switchover in opposite direc tion From the valve the column is filled with sample through the channel 2 gt 3 The quicker part of the substances is separated through the channels 6 gt 7 and 5 gt 4 and transported to the detector Fig 34 Backflushing Transport of the sample onto the col umn Make sure there is no pressure when switching so that the col umn is not damaged Assistant ASM 2 1L User Manual V6815A version 1 2 40 Legend
75. tivated for all USB hosts Only for Windows 7 For the network adapter the option Allow the computer to turn off this device to save power in the Device Manager must be deactivated 1 In Windows 7 chlick on Start Control Panel Network and Sharing Center Double click on LAN Connection Click on the button Properties Select Internet Protocol version 4 TCP IPv4 Click on the button Properties Nn BW N Check the settings in the tab General The correct settings for the DHCP client are a Obtain an IP address automatically b Obtain DNS server address automatically 7 Click on the button OK Assistant ASM 2 1L User Manual V6815A version 1 2 49 Note Legend Modules Router LAN port WAN port Workstation Prerequisite Procedure Procedure Result KNAUER Initial Startup Connecting the Cables To avoid interference we recommend to operate the HPLC sys tem separate from the company network This is why we recom mend using a router A router has several LAN ports and one WAN port that can be used to integrate the LAN into a wide area network WAN e g a company network or the Internet On the other hand the LAN ports serve to set up a network from devices and a computer A patch cable is included in the accessories kit A router is not included in the scope of delivery To connect the router to a WAN an additional patch cable is required which is also not sup
76. uisite The SmartMix mixer has been removed The mixer lid of the SmartMix mixer is opened Replacing the filter system 1 Remove the filter system in the mixer lid with a pair of pointed twee zers rl Fig 69 Remove the filter system from the mixer lid KNAUER Assistant ASM 2 1L User Manual V6815A version 1 2 74 Maintenance and Care Replacing the filter system 2 Insert the new filter system at position in the following order 1 unit filter round 7uUM 1 unit glass fiber filter 1 unit filter round 3umM Fig 70 Insert new filter system in the mixer lid Put on the mixing lid with the fittings of the capillaries Put the mixer lid with the replaced fil ter system onto the mixer housing with the cartridge and the PEEK sealing ring Tighten the four screws on the mixer lid with an Allen wrench wrench size 3 0 x 150 Replacing the Cartridge The design of the cartridges is different depending on the ver sion of the SmartMix mixer Prerequisite The capillaries were loosened The SmartMix mixer has been removed Procedure Loosen and lift off the mixer lid Remove the PEEK sealing ring Replace the cartridges Put PEEK sealing ring back on Note Do not use any metal tools to take the cartridge out of the hous ing Otherwise damage might be done to the seals of the car tridge 1 Take the PEEK sealing ring lying on the cartridge off th
77. upplied blind fittings to seal off all unused inlets and outlets Connecting Degasser and Pump Each degassing chamber features an inlet and an outlet Connection of the chambers in the direction of the arrow Remove the blind fittings from the degassing chamber inlets and outlets that are to be used and keep the filler caps Use blind fittings to close unused inlets and outlets The inlets of the degasser chambers are connected to the sol vent containers by means of tubes Teflon tubes are also connected to the outlets of the degas sing chambers with screw fittings and seal rings These tubes are then connected to the respective pump Meaning of the Degasser LEDs Two LEDs at the front of the degasser in the ASM 2 1L inform about the operation condition of the degasser KNAUER Yellow LED Shows that the vacuum has not yet reached the work range Usually the yellow LED lights up when the device is switched on and goes out as soon as the vacuum drops below 130 mbar Green LED Shows that the vacuum is within the work range Usually this indicator lights up when the vacuum has been gener ated and stays on as long as the degasser is in use Assistant ASM 2 1L User Manual V6815A version 1 2 44 Initial Startup Degasser Gas Outlet If the ASM 2 1L is equipped with a degasser the gas outlet exhaust will be located on the front Air and possibly solvent vapors will be pumped out here If necessary it can be com
78. ve Prerequisite Holding bracket is attached to the side panel Tools 9 64 Allen wrench Procedure Screw the adapter plate to the back side of the holding bracket Slide valve drive from the rear through the hole of the holding bracket and the adapter plate forwards as far as possible Fasten valve with four screws to the front side Fig 19 Back side of VICI Valco valve drive at the holding bracket Fig 20 Back side of VICI Valco valve drive at the holding bracket Result The VICI Valco valve drive is mounted KNAUER Assistant ASM 2 1L User Manual V681 5A version 1 2 32 Initial Startup Mounting a Flow Cell Prerequisite Holding bracket is attached to the side panel Procedure 1 Position the adapter plate to the back side of the holding bracket Position the flow cell to the front of the holding bracket Fasten the flow cell with both knurled head screws in posi tion Fig 21 Flow cell with adapter plate at the holding bracket Result Flow cell is mounted Mounting Axial Compressible Columns Vertex Plus AX 20 m 30 mm Prerequisite Holding bracket is attached to the side panel Tools Allen wrench 4 Procedure 1 Place the column inside the holder Position the holder onto the bore holes of the holding bracket Tighten the screws and washers Fig 22 Column holder with hold ing bracket Result Axially compressible column

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