SpinCon PAS-450-10
Contents
1. INNOVAPREP SpinCon PAS 450 10A Portable Air Sampler User Manual DISCLAIMER OF WARRANTIES YOU EXPRESSLY UNDERSTAND AND AGREE THAT A INNOVAPREP WARRANTS THAT THE PRODUCT HAS BEEN MANUFACTURED AND SHALL CONFORM TO THE PROVIDED SPECIFICATIONS NO OTHER WARRANTY OR GUARANTEES ARE PROVIDED B YOUR USE OF THE PRODUCT IS AT YOUR SOLE RISK THE PRODUCT IS PROVIDED ON AN AS IS BASIS INNOVAPREP EXPRESSLY DISCLAIMS ALL WARRANTIES OF ANY KIND WHETHER EXPRESS OR IMPLIED INCLUDING BUT NOT LIMITED TO THE IMPLIED WARRANTIES OF MERCHANTABILITY FITNESS FOR A PARTICULAR PURPOSE AND NON INFRINGEMENT C NO ADVICE OR INFORMATION WHETHER ORAL OR WRITTEN OBTAINED BY YOU FROM INNOVAPREP SHALL CREATE ANY WARRANTY NOT EXPRESSLY STATED HEREIN SpinCon PAS450 10A Manual 7 19 05 TABLE OF CONTENTS Section 1 MEOT CHON ri Op l nUT O PT TI l n ka Kone ekl ee ee ar Ke ae ann e Y ebe ew Ee ee Intended ADpDlIcall OS i L l acetate ii DCS CRD a N o ro lt lt lt rr bbn SPECHICATONS saab Sectlon 2 5 1 p and Ussesi anene eria Ar LA k DA di kaka b 331 lak n aer lak a akn BS 00 PPC e AE Eek O K e lose cece A WE ne NADIN SOMO Malo oc SECON ag Dar nc Operator Maintenant Onir ll yr A ds Cleaning E Xtermal COmDOH mt cuasi Eleanme Inter al Pluid kin xe En ico Contactor Clica SUPPI atra aa Yearly Prey entive Maitena as Section O DECON AMI NANON A A Section 5 Environmental Limitations2. and are firmly seated The connectors and bags can be removed after operations are complete by depressing spring on top of female connector and pulling out the male connectors Place sample bottle in holder Push in slide on left side of bottle holder Insert bottle without cap Release slide Press ON OFF button When on button lamp will illuminate secondary cooling fan will activate Confirm that all four status lights are illuminated one on each module When using remote unit the screen message will be Ready Set the timer to the desired collection time Push the COLLECT button on either the air sampler or the remote unit F1 Following the collection cycle after the liquid has been pumped from the contactor remove the bottle by pressing the release spring and replace the cap Place another bottle in the holder 10 Press the COLLECT button on either the air sampler or the remote unit 11 If a rinse is desired or required depress RINSE button on F1 to end the collect cycle either remote unit F2 or sampler control panel Action is confirmed by a button lamp Since water is being pumped into contactor only the pump lamp will pulse Contactor will fill and drain again Push RINSE button off remove bottle and dispose of waste Replace with new sample bottle 12 Be sure that last operation before shut down was a RINSE if not go to STEP 11 Depress the ON OFF b
3. bagged and a more complete gas decontamination of the system should be preformed using Formaldehyde or Hydrogen Peroxide in an approved gas chamber Users may coordinate with Innovaprep to provide a SpinCon loaner during the decontamination process 1f required There will be a fee for the loaner and availability of loaners at times may be limited due to unforeseen circumstances A Decontamination Decision Tree is provided to assist the user Recommended is to treat all verified hot zones as needing a complete gas decontamination of the system to minimize the possibility of internal contamination Ho Further Decon Necessary i Potential Hot 1 Perfonni Run sternal Fone Sample i Surface Decon Collection Procedure Run Field Decon Procedure or Double Bag Unit Wore Sampling Req d Run Decon Solution Rinse and Water Rinse SpinCon PAS450 10A Manual 7 19 05 19 Section 5 Environmental Limitations Keep in a dry environment The unit is not rainproof or waterproof Operate in a low dust environment Excessive dust can cause premature wear and could cause fluid level control to fail over time without proper preventive maintenance The operating temperature range 1s 36 to 120 F 2 to 50 C The operating humidity range is O to 100 Relative Humidity non condensing Chemical compounds used for decontamination of the unit may limit the life of some components If the unit has been decontaminated
4. bleach to remain in SpinCon PAS450 10A Manual 7 19 05 15 the lines for too long can reduce the lifetime of the system s fluid injection pump and cause sample degradation Alternatively if only one source of bleach water solution is available a collect and rinse may be run in succession by connecting the source to the left port and running a short collection and then placing the source on the right most port and running a rinse Ensure that both ports are rinsed thoroughly with water and that several collections and rinses are run with water to remove any residual bleach As an option an additional rinse could be performed using a sodium thiosulfate solution after the bleach rinse and before the water rinses This may be useful for prolonging the life of the internal components that had been in contact with the corrosive bleach solution as well as providing extra certainty that residual bleach will not affect any subsequent samples Rinse both lines several times with sterilized water after performing a sodium thiosulfate rinse If the unit has been unused for a period of time it 1s recommended that the same flush procedure be performed prior to use This is recommended in case any growth has occurred in the lines Note As noted in the Use section of this manual the unit should always be put through a RINSE cycle after every sample collection This is considered part of the normal operation of the unit and 1s include
5. flash of the LED indicates a 250uL sterile water injection At the end of the manually set time the blower will slow and the unit will pump the sample fluid into the sample bottle The timer can also be manually turned to 0 minutes to end the sampling cycle Note If a bottle is not present in the extraction port bottle holder the unit will wait The unit will automatically transfer the sample once a bottle is put in place Once the sample collection has completed press the COLLECT button to end the cycle Pressing the COLLECT button returns the unit to its READY status and ends the collection cycle Remove the sample bottle from the bottle holder and replace the lid sampling pressing the MANUAL EXTRACT button will turn on the extraction pump Press the MANUAL EXTRACT button again to stop the pump Beware SpinCon PAS450 10A Manual 7 19 05 10 that while in this cycle the unit will not check for the presence of a collection bottle in the bottle holder before starting 6 Perform a rinse cycle by inserting a rinse collection bottle into the bottle holder and pressing the RINSE button When the RINSE button is pressed the following rinse sequence will automatically occur e The RINSE button will illuminate and the PUMP LED will flash e After the initial injection is done the PUMP LED will stop flashing e After a timed rinse period expires the rinse
6. right front of the unit below the power status LED DC Power The connection terminal for DC power hookup is located on the back of the unit next to the transport handle Two DC power breakers are located on the upper left corner of the face one for 24 Volt DC and one for 12 Volt DC A backup breaker switch is located on the left front of the unit just below the DC supply LED status light Timer and Button Panel The Timer and Button Panel is located on the bottom left of the front It consists of an analog timer and five control buttons The analog timer is used by manually turning the dial clockwise to set the desired sampling time The maximum sampling time available is 6 hours The timer also has several adjustment screws located on the face around the timer dial for changing the resolution of the timing The user can adjust the timing units hrs min sec 10h by adjusting the screw in the lower right and the timer scale by adjusting the screw in the lower left A mode indicator is in the upper right of the timer and must always remain set to mode A The Button Panel consists of a red status LED the analog timer and buttons that control various stages of the sampler operation Lamps in each of the buttons indicate the current cycle SpinCon PAS450 10A Manual 7 19 05 Control buttons for the sampler are ON OFF COLLECT RINSE PUMP and MANUAL EXTRACT Remote Control Panel The R
7. the clear polycarbonate window using the four attaching screws Cleaning of the contactor should always be done using an oil free cloth or paper towel Water or rubbing alcohol can be used to aid in the removal of any dirt or dust from the contactor Contactor cleaning is discussed further in later sections Minor repairs and parts replacements should be advised by the technical support team Replacing components recalibration and major repairs are performed by Innovaprep Cleaning External Components The unit can be wiped down using a damp cloth with a 10 bleach water or mild cleaning solution Cleaning Internal Fluid Lines After use both lines should be flushed with a 10 bleach water solution followed by several water rinses Flushing is necessary to help minimize biological growth in the fluid lines The unit should be flushed if it will not be used for more than a day Flush Procedure Connect a source of bleach water solution to each fluid port set the sample time to 0 minutes and press the SAMPLE button This will flush the fluid lines from the sample fluid port to the C C To flush the fluid lines from the rinse fluid port to the C C press the RINSE button after the zero duration sample cycle has completed Wait for 5 minutes before continuing with any follow up rinses in order to allow the bleach to act Then rinse the bleach from both lines of the unit by repeating these steps using sterile water NOTE Allowing
8. N OFF button has been pressed Module connectors may be Check that all green wiring harness loose connectors are firmly seated During sampling Elapsed Timer does not count ending a cycle Certain functions do not seem to work properly when buttons are pressed Fluid does not extract after sampling is complete loose Unit was shut down without Module connectors may be Clogged fluid extract line Re initialize unit by turning off the RINSE and SAMPLE cycles and the turning power off then back on Check that all green wiring harness connectors are firmly seated Inspect and remove debris from fluid extract fittings or tubing between contactor and extract pump Sample bottle not present Insert sample bottle into bottle holder Defective bottle presence sensor Lights should be lit on bottle sensor and should flash red when an object passes within half an inch of the face of the sensor If no lights are illuminated or do not flash contact Innovaprep for repair Defective extract pump Contact Innovaprep for repair Sample fluid does not inject into contactor empty Clogged or leaking fluid lines Defective fluid injection pump Blockage in air path Check for obstructions in air flow path both inlet and outlet and clear Sample volumes high Dirty contactor Fluids may not be connected Fluids must be connected to BOTH the red properly or fluid bags are and blue fluid conne
9. after collecting infectious material it should be sent to Innovaprep for preventative maintenance immediately thereafter SpinCon PAS450 10A Manual 7 19 05 20 Section 6 Safety Precautions This unit is not a closed system Some air leakage from the collection chamber may occur Use caution and treat all collected samples as if they actually contain the materials for which they are being sampled Be aware that the unit should be electrically grounded and not used in situations where electrical shock is likely do not place or operate the unit in standing water Warnings DO NOT operate the unit with the covers removed DO NOT operate the unit in the rain DO NOT place or operate the unit in standing water DO NOT soak the unit when cleaning wipe with a damp cloth containing cleaning solution water or alcohol only DO NOT restrict the inlet or outlet during operation as this will reduce performance DO NOT drop the unit as it can damage the electronics and the glass C C SpinCon PAS450 10A Manual 7 19 05 21 Section 7 Troubleshooting Troubleshooting information for the SpinCon bioaerosol sampler is listed below In most cases when trouble is encountered a review of the operating instructions will provide a solution The unit does not come on The system electrical when plugged in and on off breakers may not be button is pressed activated Make sure all breakers on the unit are pushed in and the O
10. ariety of analytic methods Compatible analysis methods include HAPSITE FTIR PCR Immunoassay Strips Culturing Microscopy Mass Spectroscopy Gas Chromatography Liquid Chromatography and Flow Cytometry among others Intended Applications The SpinCon PAS 450 10 sampler is used to sample biopathogens particulates and chemicals suspended in the air It has been successfully used to find Biological Organisms Particulates Vapors amp Aerosols Anthrax Spores Military Grade Military Nerve Agents Viruses and Coliphage viral material Military Blister Agents Bacteria and Coliform bacterial material Psychotic Agents Spores amp fungal allergens Explosives Mold pollen and spores Pesticides SpinCon PAS450 10A Manual 7 19 05 Concentrated liquid samples are ideal for many detection technologies Depending upon the sampling time the particles or compounds of interest in air can be concentrated by several orders of magnitude Concentrating the constituents of interest is an effective way to overcome sensitivity problems encountered due to the detection limit of the analytical method being used Therefore concentrations in the air which would be undetectable using a conventional air sampler are now easily detected The SpinCon PAS 450 10A biological aerosol sampler is capable of sampling air at a rate of 450 liters per minute and is configured for approximately 10 milliliters of liquid per sample It operates in a batch mode colle
11. cting a single sample with each use Description The SpinCon PAS 450 10A sampling system is approximately 18 inches by 8 inches by 15 inches The system is equipped with wheels and pull handle to enhance portability The main components of interest are the power supply timer button panel the collector concentrator C C the air inlet outlet and the operating fluid and sample extraction ports 24 8 12V DC Breakers Main Power Status LED Main Power Breaker Power Application after power has been converted Mode Status LEDs Collector Concentrator C C AC Breaker C C Backlight Button Remote Pendant Operating Fluid Ports Timer Button Panel Collector Concentrator Bottle Holder Status LED Extract Port Illustration 1 SpinCon PAS 450 10A sampler SpinCon PAS450 10A Manual 7 19 05 Power Supply The main power switch is located on the button panel This is a pushbutton that is used to activate powering of the internal components and start collecting sample as long as power is connected The unit can be powered by 120 Volt AC power or by DC power using either 24 or 12 Volts A power status LED located on the upper right of the front panel will indicate which AC or DC power source is in use The unit defaults to AC power when both power supplies are attached AC Power The power cord for 120 Volt AC power is a retractable cord reel accessible on the upper right side of the unit An AC power breaker is located on the
12. ctors Check fluids referencing the manual for proper connection if needed Inspect fluid lines for clogs and clear if necessary If lines are leaking check to make sure fittings are tight When rinse cycle is activated a faint clicking noise approximately twice per second should be heard if no clicking is audible contact Innovaprep for repair Clean contactor interior and exterior per preventive maintenance procedures Clean contactor interior and exterior per preventive maintenance procedures Blower not tightly Check to make sure blower is securely connected to contactor box attached to the contactor box SpinCon PAS450 10A Manual 7 19 05 22 Section 8 Contact Information Innovaprep LLC 132 E Main St Drexel Missouri 64114 Customer Service For supplies and service Phone 816 619 3375 E mail info innovaprep com Website http www innovaprep com SpinCon PAS450 10A Manual 7 19 05 23 Section 9 Quick Operating Instructions il Plug the retractable cord into an 110 volt AC outlet or establish power with 12 or 24 volt batteries Cooling fan will turn on if using AC power Decide on method of controlling operation from either the remote unit and or from the switches on the front of the panel Install Collection Fluid and Sterile Water bags to hooks on black panel above blower motor connect male connectors to appropriate connector on sampler The connectors are properly installed when they click
13. d here to stress its importance Contactor Cleaning Note It is critical that the contactor glass in front of the level sensors stays clean An indicator that the contactor should be cleaned is if the operator begins to notice that sample volumes are significantly more or less than 10 ml Also visible coatings on the interior or exterior of the contactor should be cleaned periodically before it becomes a larger problem Cleaning Exterior of Contactor STEP 1 Remove four screws from plexi glass covering contactor box STEP 2 Carefully clean exterior of glass contactor with a cloth dampened with water or alcohol Pay particular attention to not disturb the positioning of the contactor injector nozzle or level sensors Always use an oil free cloth or paper towel for cleaning the contactor STEP 3 Most importantly ensure that glass in front of level sensors is cleaned SpinCon PAS450 10A Manual 7 19 05 16 Cleaning Interior of Contactor STEP 1 Remove contactor blower module Unscrew two knurled fasteners at the sides of the blower mounting plate Unscrew inlet and outlet flanges from the sides of the outside of the case being careful to apply pressure to the front of the blower module to prevent binding The inlet and outlet once initially loosened should be freely rotated by finger pressure If not binding is occurring and correct pressure on the blower module is not being applied Check for clearance between the
14. emote Control Panel consists of an LCD display buttons and LEDs that control and provide information throughout various stages of the sampler operation When in use a red LED will illuminate indicating the current cycle and the LCD interface screen will display status information The Remote Control Panel plugs into the left side of the unit Buttons found on the Remote Pendant sampler are SAMPLE RINSE and FLUID INJECT Trigger Port A trigger port is located directly above the connector for the Remote Control Panel on the left side of the unit This port allows the user to provide a switch closure to begin or stop a collection cycle The function of the switch closure mirrors the function of the collect button on the unit Collector Concentrator C C The Collector Concentrator C C is where the air sample is routed through the unit and the target material concentrated in the liquid phase As air passes through the C C cyclonic mixing transfers a high portion of the target particles or compounds in air to the liquid medium The liquid medium remains in the C C as air is sampled to continuously concentrate the constituents of interest into the liquid The mixing of air with water also causes liquid evaporation during sampling Evaporated water is replaced by injecting makeup water throughout the sampling process A red LED located just to the left of the C C should be illuminated when the unit has power ap
15. flange being removed and the system case and lessen or increase pressure as needed to allow clearance If excessive binding occurs the connection threads may gall potentially resulting in seizing of the inlet or outlet flanges to the module and damaging the system blower Once the inlet and outlet tubes are removed slide the contactor blower module STEP 2 STEP 3 STEP 4 STEP 5 forward and out of the case If necessary unplug the wiring harness from the green 12 pin receptacle on the contactor box to allow additional flexibility Unscrew four knurled fasteners on top of blower plate There are two knurled fasteners on the front of the module and two on the back Separate the contactor box from the blower DO NOT remove the four flathead screws securing the contactor to the box Carefully clean interior of glass with a cloth dampened with water or alcohol Always use an oil free cloth or paper towel for cleaning the contactor Most importantly ensure that glass in front of level sensors is cleaned Knurled Knobs 4 Contactor _ Cover lt Screw 4 Fluld Fluid Level Injector Nozzle Sensors SpinCon PAS450 10A Manual 7 19 05 17 Illustration 2 SpinCon PAS450 10A Contactor Box Supplies The refrigerated shelf life of sampling fluids is two months It is recommended that sampling fluids be kept refrigerated Yearly Preventive Maintenance The unit should be sent in annually for pre
16. fluid will be deposited into the waiting bottle e Ifa bottle is not present in the extraction port bottle holder the unit will wait The unit will automatically extract the rinse water once a bottle is put in place e If the rinse fluid is not completely extracted from the sampler pressing the MANUAL EXTRACT button will turn on the extraction pump Pressing the MANUAL EXTRACT button again will turn off the pump 7 After the rinse cycle has completed press the RINSE button again to end Pressing the RINSE button once again completes the rinse cycle and returns the unit to the READY mode Starting Collection Using the Remote Control Panel 1 Set the sample timer to the desired sampling time by manually turning the time dial clockwise The maximum sampling time that should be set is 6 hours 2 Sample collection is started by pressing the SAMPLE button on the keypad found beneath the pendant LCD screen The SpinCon sampling system will initiate sampling and the following events will be observed e The SAMPLE LED will illuminate the PUMP LED will flash and the interface screen initially displays Collection Cycle charging e There will be an initial injection of approximately 10 milliliters of the sampling solution This takes about 45 seconds SpinCon PAS450 10A Manual 7 19 05 11 e After the unit is done charging the PUMP LED will pulse periodically and the t
17. imer will start The screen will display Collection Cycle while showing elapsed sampling time in seconds e The unit will periodically inject water to compensate for fluid evaporation as indicated by the PUMP LED 3 At the end of the manually set time the screen will display Transferring Sample The blower will slow and the unit will pump the sample fluid into the sample bottle The timer can also be manually turned to 0 minutes to end the sampling cycle Note If a bottle is not present in the extraction port bottle holder the unit will wait and the pendant will display Waiting for Bottle The unit will automatically transfer the sample once a bottle is put in place Once the sample collection has completed the LCD screen displays Transfer Complete Press SAMPLE to end 4 Visually verify that the entire sample has been transferred and then press the SAMPLE button This returns the unit to its READY status as displayed on the screen and ends the collection cycle Remove the sample bottle from the bottle holder and replace the lid 5 If the fluid is not completely extracted at the end of sampling pressing the MANUAL EXTRACT button will turn on the extraction pump Press the MANUAL EXTRACT button again to stop the pump Beware that while in this cycle the unit will not check for the presence of a collection bottle in the bottle holder before starting 6 Perform a r
18. inse cycle by inserting a rinse collection bottle into the bottle holder and pressing the RINSE button When the RINSE button is pressed the following rinse sequence will automatically occur e The RINSE LED will illuminate the PUMP LED will flash and the interface screen initially displays Rinse Cycle Injecting rinse e After the initial injection is done the PUMP LED will stop flashing and the interface screen displays Rinse Cycle Rinsing Contactor Time Remaining while showing the remaining time e After the count down on the rinse cycle is complete the interface screen displays Rinse Cycle Transferring Rinse The rinse fluid will be deposited into the waiting bottle e Ifa bottle is not present in the extraction port bottle holder the unit will wait and the pendant will display Waiting for Bottle The unit will automatically extract the rinse water once a bottle is put in place SpinCon PAS450 10A Manual 7 19 05 12 e If the rinse fluid is not completely extracted from the sampler pressing the MANUAL EXTRACT button will turn on the extraction pump Pressing the MANUAL EXTRACT button again will turn off the pump 7 After the rinse cycle has completed the interface screen displays Transfer Complete Press RINSE to end Pressing the RINSE button once again completes the rinse cycle and returns the unit to the READY mode which will be disp
19. is removed by unlatching it from the port The system uses disposable polyethylene collection bottles Additional collection bottles may also be reordered from Innovaprep LED Status Lights There are four LED Status Lights located across the front of the unit that indicate if power is correctly applied All four LED Status Lights should be illuminated when the sampler is turned on SpinCon PAS450 10A Manual 7 19 05 Specifications Power Requirements 120 VAC or 12 24 VDC Power Consumption approximately 450 W Airflow Rate 450 L min Size 18 inches x 15 inches x 8 inches Weight 46 pounds Sampling Time Selectable between 5 minutes and 6 hours Operating Temperature Range 2 to 50 C 36 to 120 F Sample Size 10 milliliters Portability Easily transportable in protective carrying case Sample Extraction Automatic into sample bottle features bottle presence detection Solution Uses phosphate buffered saline in combination with sterile water for sample collection Controls Integral control with LCD status display sample rinse sample time and manual extract and inject controls Decontamination Exterior surfaces can be wiped with dilute bleach solution Fluid lines and contactor can be flushed with bleach solution followed by rinses with sterile water SpinCon PAS450 10A Manual 7 19 05 Section 2 Setup and Use The SpinCon PAS 450 10 sampling system operates on 120 Volts AC or 12 24 Volts DC power It should n
20. kek NECUON OF Salety Tecal On aa a Section 7 TrOUDlESDOO NNE usada eae de KEN pois desde sections Contact Info TI Tat oN Rand SpinCon PAS450 10A Manual 7 19 05 Section 1 Introduction The SpinCon PAS450 10A is a commercially available portable and energy efficient wet concentrator air sampler Tested and proven in user sponsored tests it is ideally suited for the collection of bio aerosols particulate matter and soluble vapors including submicron sized particles airborne molecular contamination biological particulates and volatile and semi volatile chemical compounds The system has been developed to address a broad range of advanced air sampling requirements and is rapidly becoming the gold standard for collecting large quantities of airborne pathogens Operating Principle A blower on the SpinCon PAS 450 10A sampler pulls air through a glass collector concentrator C C which contains a sampling liquid As air passes into the C C entrained solids impact on the sampling liquid droplets and are captured Gaseous materials are likewise dissolved in the fluid droplets The sampling liquid remains in the C C to sample more and more air As a result particles and compounds residing in the air are continuously concentrated in the sampling fluid medium After the chosen sampling interval the liquid in the C C can be drawn off as a discrete liquid sample This sample can then be analyzed for the constituents of interest using a v
21. layed on the LCD screen Manual Functions The MANUAL EXTRACT and PUMP control buttons are used as backup options Manual Extraction As also discussed in Steps 5 and 6 above fluid can be manually extracted from the C C If the sample or rinse fluid is not completely extracted or for some other reason you want to collect the fluid in the C C use this option Pressing the MANUAL EXTRACT button will turn on the extraction pump Note that the MANUAL EXTRACT cycle will not check for the presence of a collection bottle so this needs to be in place first Pressing the MANUAL EXTRACT button again will turn off the pump Manual Fluid Injection The PUMP is used to add more fluid to the C C but should not be needed during normal use Pressing the PUMP button will inject one 250 microliter pulse of water into the C C This button can also be used to add extra fluid during a rinse cycle which is useful to ensure that the fluid level rises high enough in the C C to rinse off dirt above the collection water line or to ensure that any bleach in the contactor is removed after a bleach rinse Shutdown Remove the sampling and rinse fluids from the Fluid Injection ports Make sure that the COLLECT RINSE PUMP and MANUAL EXTRACT buttons are not illuminated Then push the ON OFF button Unplug the unit from its power source The unit is ready for whatever care and cleaning a
22. ot be turned off unless all cycles are completed as indicated by the illuminated ON OFF button or by the remote pendant showing READY If power is lost or the unit is shut down before it is in the READY mode some minor adjustments will need to be made as described in the Shutdown section later in this manual The sampling system uses about a 10 milliliters charge of sampling fluid during the initial fluid injection and about 1 to 2 milliliters of sterile water per minute of sampling depending on environmental conditions Expect 2mL per minute at high ambient temperatures gt 80 F and low humidities lt 15 RH whereas less than 1 mL per minute evaporation can be expected at high humidities gt 80 RH and low temperatures lt 60 F Additionally about 12 milliliters of sterile water will be used per rinse cycle Running a rinse cycle after every sample cycle is highly recommended Setup Connect the unit to an appropriate 120 Volts AC or 12 24 Volts DC power source and make sure sampling fluids are placed in their proper ports Powering On 1 Connect the unit to an appropriate power source 2 Make sure all breakers are pressed in and push the ON OFF button 3 The ON OFF button and four red status LEDs will illuminate indicating that power is applied to all the hardware boxes Connecting Fluids 1 Hang the fluids bags on the hooks provided above the ports and connect the bag outlet to the
23. plied After the chosen sampling interval the liquid in the C C can be drawn off as a discrete liquid sample This sample can then be analyzed for the constituents of interest SpinCon PAS450 10A Manual 7 19 05 Air Inlet Outlet The blower provides an airflow of 450 liters per minute by drawing air from the left side of the unit and then through a slit cut into the C C After passing through the C C and blower scrubbed air is exhausted through the right side of the unit near the top Operating Fluid and Sample Extraction Ports The Operating Fluid Ports are found on the lower front of the unit The blue port on the right side of the C C is used for the sterilized water connection The red port on the left is used for the sampling solution The SpinCon PAS 450 10 system is shipped with an initial supply of sampling fluids a sampling solution for sample collection and sterile water for makeup liquid and rinsing Additional sampling fluids may be reordered from Innovaprep LLC 132 E Main St Drexel MO 64742 The refrigerated shelf life of the fluids is 2 months 1 month unrefrigerated The Sample Extraction Port or bottle holder is found on the front of the unit near the bottom This is where the sample collection bottle is inserted by removing the lid and latching it in place After sampling is complete the sample bottle will contain all the fluid that had been in the C C during sampling At the end of sampling the sample bottle
24. proper port Make sure that the correct fluids are connected to their corresponding fluid ports as described in Section 1 and on the system labels 2 Place the sample bottle in the sample extraction port bottle holder by simply removing the lid pressing in the locking plate inserting the bottle and releasing the locking plate SpinCon PAS450 10A Manual 7 19 05 Use Starting Collection Using the Button Panel 1 If the fluid is not completely extracted at the end of Set the sample timer to the desired sampling time by manually turning the timer dial clockwise The maximum sampling time that should be set is 6 hours Sample collection is started by pressing the COLLECT button on the button panel The SpinCon sampling system will initiate sampling and the following events will be observed e The COLLECT button and the filling LED will 1lluminate and the PUMP LED will flash e There will be an initial injection of approximately 10 milliliters of the sampling solution This takes about 45 seconds e After the unit is done filling the filling LED will turn off the PUMP LED will pulse periodically and the timer will start as indicated by a green flashing LED on the timer e The unit will periodically inject water to compensate for fluid evaporation as indicated by the illuminated PUMP LED Monitoring the PUMP LED can give a good indication of the current fluid usage as each
25. re needed For information on care and cleaning see the following Section 3 SpinCon PAS450 10A Manual 7 19 05 13 Unintended or Premature Shutdown If power is lost or the unit is turned off without ending a system cycle 1 e COLLECT or RINSE buttons are illuminated on the Button Panel or not in the READY mode on the Remote Control LCD panel the unit will resume from the same state it was in at shutdown once power is turned back on The elapsed time will read 00000 however the manual timer will reset to its full value and the sample will continue for the full duration listed on the timer If you do not wish to resume the cycle at this point press the appropriate use button e g the SAMPLE button or the RINSE button to return the unit to the READY mode Cycle the power off then on again to re initialize the system SpinCon PAS450 10A Manual 7 19 05 14 Section 3 Care WARNING Before any maintenance action unplug AC or DC power supplies Operator Maintenance Maintenance involves weekly surface cleaning checking for loose connections decontamination and rinsing of the collector cleaning around the collector and cleaning of the air passage ways Cleaning around the contactor should be performed if dust or oil is visible between the contactor and photoeyes the photoeyes are mounted on the C bracket surrounding the contactor To clean around the contactor requires removing
26. utton to shut down light will extinguish SpinCon PAS450 10A Manual 7 19 05 24
27. ventative maintenance First clean the external components and internal lines with a 10 bleach solution as previously described in Section 2 Contact Innovaprep at 816 619 3375 for a Return Materials Authorization RMA and shipping instructions If the unit has collected infectious material you must notify Innovaprep of this when requesting the RMA A certificate of decontamination will be required prior to shipping the unit SpinCon PAS450 10A Manual 7 19 05 18 Section 5 Decontamination The Field Decontamination Procedure 1s Step 1 Remove the sampler from the hot zone to an area where decontamination can be safely performed Step 2 Wipe the exterior of the case air inlets handles water PBS bags external hoses and platform around the sampler with 10 bleach solution Step 3 Run the Decontamination Fluid 10 bleach solution through the collect and one rinse cycle Cover the intake port with bleach soaked wipe during cycles Allow to stand for 10 minutes Step 4 Conduct two rinse cycles with sterile water following a 5 minute wait time Step 5 Conduct third rinse cycle with sterile water and collect sample for testing Step 6 Run an analysis on the rinse water sample to determine status of decontamination NOTE These procedures may vary according to the specific situation in which collections are being made or tailored to a specific pathogen of interest For certain pathogens the SpinCon needs to be double
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