Nichipet F/V(08.8
Contents
1. 0 3 NPV L 200 500 10004 0 7 0 5 lt 0 3 lt 0 2 Nichipet F FIXED VOLUME Standard Volume Cat No Volume Accuracy Precision NPF 5 Sub 1 5 lt 0 5 5u 2 5 10 20 25 50 10u2 41 2 lt 0 4 to 100 200 250 300 20u 6 25u2 0 8 lt 0 3 NPF 1000 500 amp 1000p 50u 6 300u 2 0 6 lt 0 3 1000u 500u 6 1000u 0 5 lt 0 2 OPERATION 1 Select the desired volume on Nichipet V by turning the thumb knob until the desired volume figures on the thumb knob corre spond to the arrow mark printed on the top of the pipette handle Fig 1 2 Place a disposable tip firmly onto the end of the pipette Depress the thumb knob to the first stop Dip the tip into the liquid to a depth of 3mm Step 1 of Fig 2 3 Smoothly return the thumb knob to its origi nal position The liquid will then be drawn Fig 1 into the tip Step 2 Arrow Mark 4 Touch the tip against the inside wall of the receiving vessel Step 3 5 Smoothly depress the thumb knob to the first stop step4 pause then depress the knob to the second stop step5 Note When dispensing serum and other viscous fluids it is necessary to pause about two seconds before moving to the secondary stop 6 Remove the used tip from the pipette by depressing the tip ejector knob Step 6 Note Depress and release the thumb knob at constant speed to ensure good pre cision RELEASE FIRST STOP SECO2. 6 pipettes can be stored Inspection and Calibration Statement The enclosed pipette was tested and calibrated under closely controlled environmental conditions to ensure that it meets published calibration specifications The precision and accuracy results obtained for this pipette are provided on the enclosed calibration certificate Because temperature and humidity conditions affect the calibration results of liquid measurement devices your pipette should be calibrated under conditions of use The calibration results obtained in your laboratory may vary from our results due to differences in environmental testing conditions For repair service or information you may contact MANUFACTURER NICHIRYO Tokyo office 2 Kandamikura cho Chiyoda ku Tokyo 101 0038 Japan TEL 81 3 6661 2602 English FAX 81 3 6661 2604 Homepage http Awww nichiryo co jp E mail info nichiryo co jp 2008 8 Ver 2 Printed in Japan
3. 2 Unscrew the barrel assembly from the Turn the screw bolt counterclockwise to remove a tip ejector pipe Gently pull the tip ejector pipe off the end of tip ejector pipe during this procedure handle and carefully pull directly away from the handle The plunger assembly and spring can be removed from the pipette Refer to Figure 4 for the internal configuration of the pipette being serviced Take special care not to bend the piston rod especially in the smaller volume units Fig 3 Tip Ejector Pipe Screw Bolt 3 Replace the O ring 4 Reassemble the unit by reversing the above procedures Refer to Figure 4 when reassembling to ensure that all parts are placed in the correct position 5 To reassemble the tip ejector pipe gently push on the tip ejector pipe so that the pipe mates with the handle connection Turn the screw clockwise to secure 6 After the above maintenance procedure should the feeling of a smooth piston stroke seem different from what it was before this procedure immediately disassemble the pipette as in the above procedure and reassemble per the schematics in Figures 3 and 4 7 After the above maintenance procedure should the tip ejector knob stroke seem different and lack the smoothness of its normal stroke immediately disassemble the tip ejector portion of the pipette and reassemble ensuring the spring and spring holder are installed properly a Handle Bartel P
4. ND STOP POSITION Calibrated Blow out Care Step 1 Step2 Step3 Step4 Step5 Step6 ipi ELE Fig 2 AIDS TO PRECISION AND ACCURACY Listed below are some techniques found to improve sampling precision PLEASE READ THIS SECTION CAREFULLY 1 Try to effect the same speed for both the intake and delivery of all samples Smooth depression and release of the plunger knob will give the most consistent results Never allow the plunger to snap back Consistency of technique is a key to precision Always depress the thumb knob to the proper stop before insertion of the tip into the solution Depression of the thumb knob after insertion may cause the formation of an air bubble in the tip and result in filling error Try to insert the tip to approximately the same depth in the sample each time never going deeper than 3mm Hold the instrument as vertically as possible 10 maximum from vertical When sampling hot or cold material the tip temperature should be equalized to that of the solution to prevent contraction or expansion of solution SERVICE AND MAINTENANCE INFORMATION It is recommended that the following servicing procedures be performed at regular intervals Heavy usage or usage with corrosive fluids will require more frequent servicing Disassembly Reference Figure 3 amp 4 1
5. Nichipet F amp V Fixed amp Triple Volume Micro Pipettes User s Manual CERTIFIED ISO9001 e Thank you for your purchase of our Nichipet F amp V e Before proceeding to work with your Nichipet F amp V carefully read this manual for proper use of it Always Pursuing Originality NICHIRYO Since 1944 FEATURES Thumb Plunger Select Volume Tip Ejector Knob Body Design Seal Ring Tip Ejector Pipe Cylinder Smooth effortless movement of the plunger reduces the operator s fatigue Volume change is simply achieved by just turning the thumb knob The selected volume remains fixed Nichipet V Built in tip ejector for the safe disposal of used tips Knob location eliminates the possibility of accidental tip ejection Lightweight slender design reduces the operator s fatigue Thermal insulation prevents body heat from contributing to measurement error during prolonged handling Teflon seal and Rubber O Ring eliminate the need for routine maintenance and lubrication Non Metallic Tip Ejector Body permits easy access to the bottom of commonly used test tubes Designed to minimize dead air space for increased pipette accuracy SPECIFICATIONS ORDERING INFORMATION Nichipet V TRIPLE VOLUME Cat No Volume Accuracy Precision NPV S 10 20 50u 2 1 2 40 7 lt 0 6 lt 0 3 NPV M 50 100 200u 0 7 0 5 lt 0 4 lt
6. lunger Tip Assembly Ea 1st Spring Screw Screw ot 1st Sprin Bolt Holder 9O Tip Seal Ring lt gt Ejector O Ring amp D gt Pipe Fig 4 1st Spring Holder Seal Ring O Ring I Fig 5 VL F 250 F 700 600u 1000p 2 ORDERING INFORMATION Nichipet F Fixed Volume Pipette Cat No Volume Accessories NPF 5 5u 5 10 20 25 50 to 100 200 250 300 Appropriate tips NPF 1000 1000p 500 amp 10004 Nichipet V Triple Volume Pipette Cat No Volume Accessories NPV S 10 20 50u NPV M 50 100 200u Appropriate tips NPV L 200 500 1000u Tips Autoclavable Cat No Volume u Color Usable Model Q ty bag BMT SS 0 5 10 Clear NPF 5 10 1000 NPV S M NPF 15 200 BMT SE 2 200 Yellow 00 NP7 8S 12S 8L 12L 1000 BMT SG 2 200 Yellow NPV S M NPF 15 200 1000 BMT L 100 1000 Blue NPV L NPF 250 1000 1000 Racked Tip Autoclavable Cat No Volume u Color Usable Model Q ty box BMT SSR 0 5 10 Clear NPF 5 10 960 96pcs x10c s NPV S M NPF 15 200 BMT SER 2 200 Yellow 00 NP7 88 128 8L 12L 960 96pcs x10c s BMT SGR 2 200 Yellow NPV S M NPF 15 200 960 96pcs x10c s BMT LR 100 1000 Blue NPV L NPF 250 1000 1000 100pcs x10c s Stand Cat No Description BM STD Acryl resin
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