User Manual - Core Life Sciences
Contents
1. Block dimension Block dimension 7mmx7mm Unit mm Unit mm R Printi A Printing starting point ae 4 Fig 3 Printing reference for using 4 5 SmartGrid SmartCover b 8 sample SmartCover c 10 sample SmartGrid SmartCover and d 12 sample SmartCover SmartCover 2 SmartGrid adhesion Select the appropriate SmartStick for the SmartGrid being used For 4 sample grid use SmartStick A Cat No 430081 for 10 and 12 sample grids use SmartStick B Cat No 430082 Note To better fit the slide the width of the trough could be adjusted by reposition the lower black strips controlled by the 3 bolts if using the top left point of the slide as the printing starting point End Upper Barrier Strip Left Upper Datumline Datumline Bott Lower Strip 2 1 Remove the protecting kraft paper from the SmartGrid Fig 5 Be careful not to damage the glue layer If the grid sticks to your hand do not pull hard Tweezers can be used to grip the non adhesive edges of the grid and place it into the trough Fig 6 Fig 4 SmartStick features Fig 5 Removing the Kraft paper from Fig 6 Place SmartGrid into the the SmartGrid trough 2 2 Put the grid into the trough with the glue layer facing up Level the left and upper side of the grid to the left and upper datumline respectively2. CapitalBio SmartGrid Cat No 430031 430033 CapitalBio SmartCover Cat No 430041 430044 CapitalBio SmartStick Cat No 430081 430082 CapitalBio SmartPress Cat No 430090 User Manual For Laboratory Research Use Only Not for Diagnostic Purposes CapitalBio Corporation General Introduction CapitalBio SmartGrid and CapitalBio SmartCover have been both developed to aid high throughput processing of multiple samples 4 8 10 and 12 samples together on a single microarray slide under identical conditions Fig 1 SmartGrid safely separates different samples added to individual blocks avoiding cross contamination while SmartCover helps to distribute the reagent solution evenly inside each block enhancing signal repeatability and minimizing variability of hybridization or immunoreaction Using SmartCover can also simplify the manual addition of solutions to the microarray Fig 1 CapitalBio SmartGrid and SmartCover Precise positioning and adhesion of the SmartGrid grid is required to create a compartmented slide and avoids any possible damage to the printed spots during this process CapitalBio SmartStick multi sample grid alignment tool aids aligning the grid to the slide surface in the correct position CapitalBio SmartPress is a grid pressing tool which ensures good adhesion of the grid with slide surface The combined use of these two tools guarantees firm accurate positi
3. Fig 6 2 3 Carefully place the microarray slide into the trough with the spotted side facing down Firstly place the end without the barcode flush to the raised end barrier then slowly lower the slide into the trough aligning the edge with the black guide strips Fig 7 Progressively contact the grid positioned in the trough until the right hand barcoded end of the slide is lowered fully onto the grid 2 4 Important Carefully press on the back of the slide with the forceps to ensure contact with all glued surfaces Fig 8 Be careful not to press too hard as to damage the slide Fig 7 Microarray slide alignment the Fig 8 Pressing with a forceps SmartStick 2 5 Check by eye to see if the grid is aligned correctly on the slide and that the grid is firmly adhered to the slide before removing the slide from the SmartStick 2 6 Place the slide into a trough of the SmartPress with gridded side facing upwards Holding the SmartPress template with one hand press the grid firmly home against the slide surface with 2 3 uniform motions of the roller Fig 9 SmartPress template Roller Fig 9 Press the grid home with SmartPress 3 SmartCover positioning Remove the PE film packing of SmartCover If plastic fragments are found on the SmartCover blow them off with an air puffer Place the microarray slide in an appropriate reaction cassette with the printed side facing up Fig 10 Fi
4. g 10 Positioning of slide with SmartGrid in IncuSet protein incubation cassette Put the SmartCover onto the slide Make sure that the side with the protruding features faces down Fig 11 Solution Port if SmartCover gt A Fig 11 Positioning of SmartCover on the slide in IncuSet 4 Reaction reagent addition Take up the reagent solution using an appropriate pipette Put the pipette tip vertically into the solution port at the corner of each block of SmartCover When the tip just touches the slide surface slowly release the solution to fill the space between the protruding features and the slide surface Fig 12 Take care to not introduce bubbles The recommended reagent volumes are listed in Table I Coverslip J J 2 Chip Incubation Cassette Fig 12 Schematic diagram of manipulation Table Recommended reagent volumes for each block of SmartCover Type of SmartCover Reagent Volume eal arene 1 sample 8 sample 18 10 20430 sample 20430 1 Maintenance 1 CapitalBio SmartGrid and SmartCover are one use consumables Repeated usage is not recommended 2 CapitalBio SmartStick and SmartPress should be cleaned with a clean piece of towel or tissue paper 3 Store all items carefully in a cool and dry place and avoid contact with water oil and other chemical
5. oning and strong adhesion of the multi sample grid on the slide surface This prevents any possible cross contamination caused by liquid sample leakage a b Fig 2 a CapitalBio SmartStick and b CapitalBio SmartPress Recommended Protocol 1 Probe printing Perform probe printing on the microarray before attaching the adhesive grid SmartGrid dimensions are given below Fig 3 for printing reference When laid down on the microarray spotter platform it is recommended to use the top left corner of the slide as the printing starting point SmartGrid Slide 1 f Poi 1 f T 1 l F pa eg 9 EJ H k L Loc Earcode 18 i 15 13 5 19 5 13 6 12 5 13 5 13 13 5 t di ion Largest array dimension 3 3 5mmx3 5mm ere mmx3mm i Protruding feature dimension _ Protruding feature dimension Li 7mmx 7mm 7 5mmx7 5mm Unit mm Unit mm Printing starting point Printing starting point a b SmartGrid Slide SmartGrid Slide GOC a GEEEEES Largest array dimension 2 5mmx2 5mm Largest array dimension 7 2 5mmx2 5mm 16 Protruding feature dimension Protruding feature dimension
6. s 4 After using for a period of time check the SmartStick to ensure the 6 bolts have not loosened Retighten the bolts with an appropriate screwdriver if loose Information Chip Hybridization For convenience and high signal reliability hybridization is best performed using a CapitalBio BioMixer II Microarray Hybridization Station Cat No 120030 and HybSet Microarray Hybridization Cassette Cat No 420010 which both help to reduced edge effects The enhanced quality of hybridization is attested in recent publications such as Patterson et al 2006 Nature Biotechnology 24 1140 1150 and Shi et al 2006 Nature Biotechnology 24 1151 1161 CapitalBio Corporation 18 Life Science Parkway Changping District Beijing 102206 China Tel 86 10 80726868 Fax 86 10 80726782 globalsales capitalbio com www capitalbio com
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