00:52, 18 February 2009
Contents
1. ln this moe samples are injected nto the Prep Valve and sige is monitored by the detectors via the acuve splits Fraction age collected bythe IRC PAL dete by ChenSttion tho und the Agile Tam AFC System configuration Analytical mode Si Ble nts de samples are inject ato the Anayical Valve The active spite i tuned all fy goes 16 the detects Thee no faction coleton BESSEEE F796 TBS ate Sng ay Ra paS UI GUN wS a CSTE So sscons ne ma anes purpose mesa andor oun S Samples may be run iter analytically or pepraive a because the autosampler can aces al poss he actin collect actions my be analyze anally afer election Agilent can suply A2PREP sotware which can automatically complet 1 Pre Analytical eu which quouesquaiying samples forthe Prep pase 2 Prep whic purifies the sanplesaconding to the pe elcted purification metod 3 ractlon ee amlysis where fsctons ate re iaectod wo the analytical syste fr confirmation Prep Applications Higher Flows upto 100maiaue Wider toes ae requied forall gu paths Everything nthe PAL inthe Prep flow upgrade I9gauge2. This shoul be under the clas of objects called INJECTORS and have the name Delay case sense Wis easy to copy the Waste object and eam tthe reteach th postion Sette Z Tolerance parameter to 0 Aju the XY poston ara pecttion depth very careflly asthe aoe must make 3 goa Seles the detector at quite high ow eats Test the seal wih she rep pumps rung by selecting Move FC to Call Pos inthe ChensStatioa PAL mem This moves the PAL to this point abd opens the aw Tostop the ts select Move FC w Park inthe ChemSiation PAL me Setting up the ChemStation Calheation Method There ate wo ses ta eting up delay eairsion wing the LEAP IFC 1 Mody the Ran Tine Ceci ia he Cnt tad on DGCALPS M math Ener th preand pos Bean macro as shown athe dialogue hee ioatoatatar F brstequin Sneed catenin te foal F Seeirboe pe nCinmad Mae oietoara Santee ES EE Ee 2 Mavy the sequence AFCDelay withthe lcaion ofthe viel containing the calibaon dye sample Performing the eabeation Select the Diagnostic view from ChenStaton When within Dagnowics select delay Volume calito agra ne EP cee Chemstaton will hea compete the elation proved The PAL fraction collector using the re run muro FCP_MoveFCTaCalbPoat will veto he Delay Sas position and raise the plunger to allow ow to pats to he sensor and wo w
3. eran Ta place a ove ne woe xiang Hel nmap Remenir tat les coped fem CD s ave read nly attributes and hes unchecked in he ile properties afer itis copied Nove fore is no User ae le iis posible check if FactPAL wil aus conetly from within ChemSttion by typing inthe above line dec ito the command line of Chen Staton Ieuparating FeactPAL at an exiting site fest go trough tbe Windows Contd Panel ounisall any previous versions Vou may wih backup or ene the signal PractPAL folder before doing 30 Do not simply copy individual les ules specifiy tol to do so by LEAP There ae several ies which mus work together and doing so inconcetly may feat infil ncompatibiie
4. Valve inlets ports and rotor grooves 9p net sleeves ve 10g syringes ml 2 Sm and Sa Injection head comes with ills ut needle guides Larger ote plas capilaios on wash tation Fraction Collection adaptations to the collector PAL Dilute type Side Por syringe assembly seas a valve Needle never touches fa sample no wash tation Special ower needle guide design Trays are defined with no pencuaton nee must ler Vals Grounding wite is essen fo safety wth high Naw rates Delay sensor on Waste station wired to Agilent fraction collector System Requirements Computer Workstation The compute worksttio i the centri forthe wer The FeasPAL sofware Wille ade ono wlcontal he configured componen tbls via pia RS 22 port Te compute shuld be PC and have lof following feaue 1G Mit on Proceso or qualent sinitoc kant COMB Hard dk Mery for appli adtonl asd fo files CDROM bine 2 0 pn CON Ports Windows 2 or XP Opecating Stern Software The Chemtation software i supplied by Agilent and mast be B O1 O1 orate The FracPAL sftwae is incompatible with eae ChemStation versions Agilent ChemStaton controls the Agilent 1200 system nd the injection PAL I below ChemStaion Version B 03 01us the appeopeate CTC diver istaled a ChemStaton log with the CTC ICC Lstament Cato Conpoaents included with te IC PAL Grin te version B03 01 or ater he Agilent CTC Conte Software purchased rms aalea Fraction collection is co
5. continues wo dispense factions when stopping a Chenstation etd ice most kely de wo Delay Calibration being performed and Chemsaion dosed and reopened ther Possibte problems 1 FraetPAl does not see end of ran ands til wating for mex fraction signal us possible ther isan emoe mesape related o snaer device Look under er szens for such exor messages eg alive split Workaround Stop the FractPAL mast and estat it agin Wort cas clase advert PootPAL Message that other COM objects are runnin or sytem hang at lac When Chemtaton abuts orcas the CTC PAL divers usally oot loaded correctly and wil proven e uoching of ChomStation Workaround Inthe Task manager lok tse if the CTCSingle ID peoess still unin aa iso stop a Allentels reboot he syste FractPAL Installation issues 1 The Useranae fle whichis lcsed in the Core oer under ChemStatnis isportat in lunch FrctPAL with Chemiaton This fie used by ChemStauon to launch optional tatu maces ad mayo may aa exist kpeoing on wheter theese any ether applications being handed by the ChenStauon application This ile tbe poset and contain the following line lunch PractPAL op with ChamStaton Macro CAProgram PllesWLEAPTEC PractPAL PractPAL23 mac 0 Whee the Path pias tothe cusenly iastilled versio of FacPAL A default version ofthis files poset onthe PctPAL installation CD which nay be copied and used as requied Do nt use hie le i ere ivan exiting
6. supe ay te etned with ows inthe same manner a a miroir plat Row length sin the ection Noe this only alfests the injection PAL Delay calibration des not move the Collector PAL to he Delay senso Remeber o modify the Run Time Checlist inthe Delay calibration tho vormaly DGCALPS AC Eater tbe pe and ostun macs as shown bee he FCP MoveF CtoCalhPort oss FCP Move CtoPark Delay calibration fils with an out of range error The installed version ofthe Sequence Fle used fr delay calito i ha Its instllod ss abit le Its wecssry to recreate it beloe ican be un To do so find he file in explonerechem 2 lwequencefedlay s Fost change om Res Only thea delete From within CS go NEW SEQUENCE ad single tine wid cation of delay sample check Pourber cote and sample nae Delay Caliban and se as AFCDELAY S Dhe following error has sometimes been seen when ChemStation s launching masatammmirias BF ya ernie centre titania aise ne s s When the furbernfrmation scren i select the fllowing message appt soars SERRATE Base tg TSP Motion tenet everclear pa le This eeor causes ChemStaton to boat apd leaves the CTCSinglID proces unig routing a feo ofthe PC It hs ben dtertined tht hissed by ation inposed in the CTCani fora maximum of 3 Trayholers and the mewage i wiggeed when 4 Traybolder as heen eesed on the PAL The oaly woearud uci sto ai having hs may tay bales fhe collection head
7. Agilent LEAP Purification Solution Based on the CTC IFC PAL us eA vy 2004 Allrights reserved Technologies LEAP IFC User Manual Table of Contents oveRVIEW SYSTEM REQUIREMENTS INSTALLING IFC HARDWARE INSTALLING FRACTPAL SOFTWARE LAUNCHING FRACTPAL CONFIGURING FRACTPAL DELAY CALIBRATION FRACTION REANALYSIS FRACTION COLLECTION INTO STACKS APPENDIX 1 INSTALLATION TROUBLESHOOTING NOTES PAL INTERFACE CABLE 2 3 16 20 Overview The Agileat LEAP purification system consists of an Agilent 1200 prep LC system pup ad sktecies comuoliag 4 CTC IFC faction cllectrlajecto PAL The IFC sample injection Functions are handled within ChemSttion using the standard CTC PAL iver Te fraction collection functions ofthe IFC ate conlled using an aditonal tm sftware packige called PractPAL FactPAL ie Heense software package font LEAP Tecnalogis Tis software must be installed separately afer CheaStaon has bee aslo The softate is unched automatically when ChemStatin is stated afer bah software packager ave installed sod configured comet The FractPAL softwae hanes communications between ChenStton the Collet PAL and the Agleat 1200 Automated Fraction Collector AFC which sends signal whenever a facto tobe cllestl TheIFC PAL The CTC InegatedFrscton Collector IFC ian autosampler ad faction clleeioe His 2 Twin PAL mounted on sinpe horzotal beam Both PAL have their wn lectonc ho
8. ards su compel log wih Separate panels Fractn Collect PAL Injector PAL The Injector PAL The CTC PAL autosampler onthe ight han seo the IBC behaves 2 a independent nit and nay be etulled either with Cyele Canposer inked i Chem Staton o she internal CTC PAL tive within ChemStton There ate no significa changes rogue ta the sftwate for atosmpling The autocunpler ean andl both vis and wel pls rea aces al poutine on the IPC including the collected featon The Fracton Collector The left hand sie ofthe TFC isthe ration collect which has fie njestion Read sccomuodat the side port syage abd colection bing I has special new puie felletion Ides aot Madea sample and doesnot hae awash station Faction collection is inated by raising the plunger ofthe side pot syringe When the plunger is raise above the lower Inlet po Now i ducted downto te collet abe When the plunger is bao his postin wis eet Waste va the pper prt Thee i an O ing seal st the top the plunger to prevent eakage Fraction colons wigered by asic oni signal om the Agilent 1200 AFC The Agilent 1200 AFC is operating normally Fis collecting inn 3 44S0mL tbe wy vita ube volume matched ote TFC abs Af ac ute ARC tay wil be et ia software so thatthe way is mver ile Ran Si Run Bad Wa t stem
9. ate At the end the ChemStatoncalivtion ocean the ost tn macr FCP MoveFCToPatk Wil ower the PAL fraction collec plunger to sepd the system aw del to waste ae wll then move te PAL faction eller ut ack othe pak position on the eft hand sie af the 14s importa close ChemStaton wo pat al of He configuration for errr states Fraction Reanalysis the customer wishes to do ction reanalysis then it wll wobbly be necessary 0 asta be Injector with singe which has a4 sch edie o each te botom ofthe rscton tubes Tis resents some special sues for setup which mus be aldvesse This ong neces exposed at Protected by the noede guide and suscepbl to damage Special ere shouldbe akon when teaching postions Accesso faction tubes by the Injector head ques that his PAL be taught the reference point othe Tesholdr called Slide G or UF and thatthe cllector Tay Types e nl i the ject set This PAL cannot reach the nna Slider ference point its ow fat the ley 0 bia eference point hasbeen elected at the ea rg comer ofthe dip ay CTC will ince acontal reference pia on the dip wasn Fue struments Teaching objects withthe Long needle 1 When checking postions Clear the Z axis ptamter 2 when aot sure i it wil be too aeep Ziolerance seins fr al abjets mst be set to zr 3 Posto for al objects should best the tp of thence imagine the nee guide its noma positon 4 ZRevact must be a value which will ensure the lng ned i etre enough nto the i
10. e aplication Objects which must be aces by the Injection PAL Prop Injection valve Analytical Ijeton vale i using Fast Wash Station Sample Tag s 4 eaction collection ways Objects which must be acenible by the Colleton PAL Fraction collection ays Cater Waste sation Not that bah tbe sample ways athe faction collection ays must be within the Working range of the injection PAL This egies careful placemat ofthe suppwt ess ensue rowril inorder to allow sfc oom forall he accesible components Signal stings onthe Colleton PAL hee are not def TTAnt Active State Low ts active when side i filly foeward TTnz Acive tte Low ts active when side i fll backward TT Ans Active State High Fraction cll signa om Chemstaion Signal stings onthe Injection PAL these are nt dal TMalnt Active State High TTaAnt Active tte Low Tre ison abou lem of slack onthe rai ome hese requirements In deo help inthe posing ofthe support eps which re the Fist step inthe hardware aston te the following pictures as guides Callector side Collector side supa le should ine up wih the sere tthe et ofthe poser connector This Wl eave room onthe jet side Tr the following aangement of As tector ste Note tht the ave picture bows only a Prep vale The independent wash ote teacket shoul alvays be noted between these legs arte the Prop valve cant ened inthe working ange ofthe PAL Ifa snl vale sad test be
11. etn2 asthe Sample stat inp sgn instead of Nave See sees belo Note When PAL signal seings are changed itis necessary to close aul ee apen FactPAL Appendix 1 Installation Troubleshooting Notes Notes on PAL Hardware Setup 1 Sample Tray extenders very tight when mating to the Tay had platform Is Sonnatimes necessity to fle the ee a litt allow i tof 2 Delay sensor board nes 10 be dill fr moustng This wil be dane atthe LEAP oie i ase cun be caine by LEAP ahead of tine 3 Tray types may nz to be uploaded othe PAL fem are sing Object manager 4 The Home positon a the Injector PAL aces tobe made safe so hat if power is turned off the lng nese will ct fall onto ay objects and be damaged 5S For the same reason as ia the Change syeage postion or both PAL needs tobe nade sf 6 The Collection PAL must conan a Wash object in the Fanwae for Cleanup Step purposes Tete willbe an eaor when opening rctPAL tht looks PAL ERROR Err Code 2 RetCode PALzetur eror 11 Objet not found using a Fixed Gilson Rack ad the Collection PAL sill ies 10 move the be then eaove the PATH Back Rest fom th mware onthe Collection PAL 1 Minimum Default Syringe volume wo le for Delay albvatio fr lage yeinges Agilent equtes Sul 100ul ange Lower the minimum syringe volume al oa the Ijector PAL 9 Ifthe Colleton PAL is diverting factions wo waste and wate to featon tubes then ont the Collection PAL fiate and conan thatthe TTL In3 Acti
12. mounted between the support eps of he Sample You wll hen Gnd tat the valve teers with thereat ost positions on the Tay olde This problem is reslved by Snsalling forward extension to the holder as shown belo Spl Ty baler tenn Install the extension by fst removing the uy boklersuppoet es from the tay platfor then easening with th extension inserted betwsen the ny lps and the ty plato ss shown above LEAP FC Ur Mama we Installing FractPAL software Before doing this inallition its acer of remove any preexisting versions af FracPAL To do ts click Start Coatol Panel gt AdMRemove Programs Select FracPAL fom the lis nd move the rogeam Nex Insert the BacPAL CD into the dk ie te After th FractPAL splasscreen thi wll the fst window you se Type in he Hiense key Toul in your CD ease apd Chck OK to ceainue Note tht the hey i Case Sse The following sezoni then displayed which allows the wer to select the operating node ofthe software For the Apa LEAP IFC sstem you MUST selet he second opin This is ot he deft selection The Gis options wed for a standalone version of the saftwae wich s doped for analytes extom ellen and reyes fete hardware Launching FractPAL The program will ad when ChemStatio i started I may also be launched om the PAL Stat FradPAL pull down menu ia ChenStation The program il nally search fr the elect PAL on COM Iiteann establish communication i will
13. mvoled by FatPAL which i ftom LEAP Techpolopes While FrictPAL integrate ighay with ChemStaton itis a separtely licensed package and is bo provided with he IFC PAL Installing IFC hardware The IFC PAL should be installed in accordance withthe CTC Manual The system ay be asl in 2 possible cntsguations An yal to Prep This system has injection valves Vale 6 fo pep injections arg volumes The fast wash sation is mounted 1 the 0a his alte dive Vale is for analytical injections Preponly This system has only 1 injection valve and is dedicated to Prep runs aly Interface connections For either configuration there ate 2otertace cable required see Appendin Injector Connect intesfae ofthe Injector PAL tthe Remote input of any Agilent module Standard Syne cable for Agent I200PAL stem Calter Connect the interface 2 conectr ofthe PAL tothe BCD Connector on Agilent 1200 BCD bose Com ports Each PAL sequts its ova RS232 ull modem cable which is supplied with the system The dealt stings expect the Injector PAL be on Coma andthe caller PAL 1 be Com2 These canbe changed in the soateconiguatio foe Com ok IFC PAL Setup There ae some special considerations when sting up the IFC PAL forthe LEAP Agilent applications The CTC manual cover basi hazdware installation ses this Seton describes the proper aangement of the configured abject the cou al 038 meet the special requests a th
14. njection Read ast not lit any objets Z Revact 1600s a god Sting pia Aft this setup as en extablished ts esto create a peal firmware backup ofthe Injector PAL using the Loader program allo eas siching between long and shot needle configurations When completed thete should bea fntware backup ofthe faction collection PAL and two fimare backups af he injector sie one foreach acd length Fraction collection into Stacks eis requied wo do fraction collection into PAL Stacks then itis necessary to ensure hatte injection oes not take place the coleton head sin place over the appropriate plate Others the Injection took place inet the ine eure to open a deaver abd position the head mabe 19 Tong the ist action maybe massed Ln ode to abieve ts here a seme spol configuration anges which ce te Injector PAL to wal at the vale foe injecting Support a PractPAL Version 22 and above Special Injection cycle in Chemstation Theresa special evel equedealed AFC Cla andi supplied on the FratPAL CD This eee contains seme ext syehtonzation commands and eeqies some sy igal seta obe changed on oth PALS Iisa cesar io change te ittace cae she patways ae lea provided i he exiting cable Signal stings on Collector and Injector PALS Caller eat TTL tn2 wil be wed ActiveState Low Ou Signal ajetd will be used ad is sotto TTL Out Injector Syne Sigua Majer s set TTL n2 Active Sue Low the FractPAL Contiguntion you mus slot TTL
15. pong the wer wo select another COM port oc to run Offline Whes onnectod i will dovnload ll the Tay objets rm ht PAL al display them nthe mala sereen belo ractPAL main serven Traybolder View The postion colored blue i the net election pion Previously collected fractions ae shown soup with diferent color for each sample If anytnys ate exclu fom the collection Soqueace se Ltr thy te paved atu stan in he sexe blo 1 FeatPAL i unig in One moe hen elas avallable ay stor df st fr fis time connection wll be dispajed The lower ight come f te sccea wil show Siulation mode tractive hea in normal operation he LED onthe lover ight of the status line wil Comma indicating the peesene os PAL td the Sats ne will sy Wait CS which indicated 3 FractPAL method is runing and he program is waiting for wseuctioas Hom ChenStation Configuring FractPAL Tray collecting sequence can be changed when inthe Method View The sequence of eolletion twas depends on the stom the eight seo the Fraton Destination Tay ues To e ede hs s tr emove tas rom the collection sequence use he buts between the bases aly 08 he Fight side wl be wed Ga the sequence they ae Ist hemes Sioa Flu me betwen samples isthe length of eto open the flow 1 waste after finishing the collection ofthe last eaeton oa sample This cleans cu any tesidal sample fom the sytnge ad needle of he collecting singe tis po
16. roeate COM prt forthe Callecoe PAL Frac PAL Configuration dialogue Lutes Options These re advanced setngs and sould only be configured by LEAP Pessoal Quick off and Full of option determin whether the clletr plunger is pushed al the way doves between actions o ist a enough wo stop How The former mode ensures all action ie epost inthe tube buts slows down the move between factions FractPAL Options dlalogue In eases were the caller bed hs oevand and backward poston the options under Tey older options need to be set ceeingly fo both positions und saved Access by pth will tray holder Nowe adcutes he beds Hed Once al ofthe Teas ate defined and the stings ate coined itis essential to save the FraiPAL defult nthod FCCHEM ce Otherwise thre wil be menage tha the ys ate ao configured popery a sarap Delay Calibration This calbeaton stp shouldbe done by the Aplent engineer a stallion and again by the asioner whenever the liquid path to the Collect sified Ils away for Chen tanta ermine wit igh accuracy the tine required for lo rom the eolumas othe coleton poi This measurement is imprint wo assure acute and etficiet collection of peaks His Important tht afte the delay clivaton i done that Chenstaton be clousd un sped a Ml al othe seins tobe uplaed Loreto sates A special Apion sear i tounied acar othe Collector waste station on the PAL This nist be define as anew abject in the PAL
17. ssible to change he collection moe fr the ations Using the Tay Sting bans svaiable i the Tey pulldown menu selection hee affect ll a Matile Fl wil pers you to collect factions iat the sme as when you have made null injections ofthe same sample The Penetration in mms the distance that the ace wil penetrate novia The default of 2m wil leave the Beale above the seceiver I your receiver vial has capo LEAD FC Ur Mama ue ap a that acd to be penetated then the Penetration distance in nm wil have to be incest ton desired poeta dep There ate a numberof Pulldown menus items in FracPAL which allow yout change the oafguration stl way the program works There ate TWO basi views a which FscPAL can porate Display Modes Access those rom the Try pulldown ea TrayHolder View This isthe default vow shown above which shows the se the fll bed layout with all configured says Tete tts linc ad tas indicator at the batt ofthe scree Only set afanmation povided Method View Ths view i msinly usd for roublesooting and roves ional infomation about the sta ofthe prograneisaso the oaly place where certain configuration opons ae slab LEAD FC Ur Maa nu Primary configuration fantins canbe sccessed del rom the mai pull down men in her view Thee te wee ilies Cont Conti is where communications stings ae set Des should noxmally ot be mnie fom their deat values except for selecting the upp
18. the file related to contiguig custom way types which wil ok lke the column onthe let Et with he appopiate definition fo the unsceo tay type Dua forget change the Pltesn ne to fleet ttl numberof bes afer your ation Cicini bore Gein ater Pate dl ca dns lt Paeeavreotnza crores rats Voe7 14 C7Cro0 Puctcrisssisctcrrs PieitFeso 25 1 cTcPERR Noe The ast prance ia the lines undrind refers ote graphic representation ofthe tay ype show on the sreen when he uy type le eleced in CheSation For cstom Ways 0 Ba STS he apc TeTS CTCPERI WG pap Plate UNKNOWN Chemttion Troy mapping changes for unexplained reasons or Tray are not mapped in logical ander Ithas toon determined that ChemStaion maps ways in ascending sequence depending othe pysicloctin of the Sot once is rm at eto reat ht Huy pins at changed then mapping wll hagge There iscutenly 0 way to Wak round his When sampling for injection ChemStation hax ben sen to aspirate sample from Incorrect locaton This has ben determined to he cause by the way Chematon converts PAL ty sons it lph umeric coors This conversion sol done cece i the PAL Try spes ar set up sothat the PAL Rows ate in the X det eft sigh Sone PAL Tay ypes eg VIZ and Fraction ways have the Row engi defined ia the Y azecton with nuberingofpositons oat to back Currently ChemStaton not handle those correctly The Workaround so make sue ll
19. ve State Sto High action collect signal iom ChemStton isso fal omar ChemStation Issues Tere nw a plc avaible ho Agen which es he rte robes 1 Delay calibration falls the APC reports Delay sensor i ot detected Consider downgrading the Product type ofthe Agilent feeton collector rom ype B to Type A to void sensing delay senso This effectively changes the way the AFC is eeopnizod by Chemstaio making i bev ikea old ile GLS64A which did have delay sensor and hence would ot look for it ding the delay cliation proces Although hie tp snot eset not doing i equie thatthe AFC dey Sensor must bel place for the calibration to compete Or gi objet such a 8 rolled up busines cat or il with aps taped tot in space Tomake this change yp ia athe command ine print sunduodutes Lafe1 RIPE G1364A csce sensitive Close Chematon power eycle AFC and estat ChemSttion You wil need 10 remove he GTSOTB Tom the coafiguaton seen whan pops up and athe CGISGIA should now be colored geen in the left hand Uist svalable mules Beear messages such as the one shown below are seen during the Chew Station sarap sequence oy lfc artnet This means hat the reported PAL Tray types te not defined inthe CTC fle Most comanen tay tyes ae dined but few ate nt tl ny custom ey types Wil povake this ere Workaround Et the CCA To a 0 te ein the Chemation PALDATA folder and open ita Nop Find he sect in
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