SPME Fiber Conditioning Station for Agilent GC Injector User Manual
Contents
1. The gas flow for the two positions is pneumatically separated Both channels can be used independently of each other The restriction is a frit installed in the SwageLok union at the conditioning station housing connection to the gas tube In case of blockage clean the frit with an intense flow of gas from the reverse side or wash in an ultrasonic bath with an appropriate solvent An aqueous mixture of an alcohol is recommended for washing as good starting point 30 SPME Fiber Conditioning Station for Agilent GC Sampler 80 120 PAL SPME Fiber Conditioning Station Operation 3 SPME Fiber Conditioning Station Operation After completing the installation it is necessary to restart the PAL system With this warm start the Agilent GC Sampler 80 120 software recognizes and references the SPME Fiber Conditioning Station Flammable and explosive gas Hydrogen is highly flammable and explosive at certain concentrations gt Use an inert gas such as helium or nitrogen for the flush gas The local SPME Cycle operated standalone contains the parameter Fiber Bakeout If this value is selected time gt 0 the Agilent GC Sampler 80 120 System Injection Unit will go to the conditioning station after the timer for Desorb Time has reached zero The fiber will be exposed for the length of time remaining for the GC Runtime A time setting of zero for Fiber Bakeout will automatically ignore the use of the SPME Fiber Con2. a LHS Upgrade j Maldi Option Firmware 3 x x j Multi Position Valve Drives Firmware 2 Output Expansion Box Firmware 2 4 0 D Solvent Reservoirs j SPME Upgrade D Stack Coolers D Stacks m Send selected Object Lists to Pal 7 Figure 4 PAL Object Manager Choose Object List Folder 2 Select the folder SPME Fiber Conditioning Station and choose the SPME Fiber Conditioning Station Option according to the available AUX Interface s SPME Fiber Conditioning Station for Agilent GC Sampler 80 120 23 2 Installation of PAL SPME Fiber Conditioning Station PAL Firmware and PAL Object Installation for PAL SPME Fiber Conditioning Station Option 3 Send the selected PAL Firmware Object List to PAL NOTE It is assumed that the PAL Firmware has also been prepared for the SPME technique If this point has been missed complete the following steps Select the class SPME Upgrade in the Object List Folder then select PAL SPMEOpt from the Object List C Program Files PAL Object ect Lists Rev JNSPME Upgrade Choose Object List Folder Object Lists LHS Upgrade p Maldi Option Firmware 3 x x SPME Out of Tray for GC PAL Multi Position Valve Drives Firmware 2 Output Expansion Box Firmware 2 4 0 Solvent Reservoirs SPME Fiber Conditioning Stations Stack Coolers YF Stacks Syringes Tray Coolers Tray Holders Tray Types Valve Drives m i Send selected Obj
3. if not correctly per formed or adhered to could result in damage to the product or loss of important data Do not proceed beyond a CAUTION notice until the indicated condi tions are fully understood and met A WARNING notice denotes a hazard It calls attention to an operating procedure practice or the like that if not correctly performed or adhered to could result in personal injury or death Do not proceed beyond a WARNING notice until the indi cated conditions are fully under stood and met SPME Fiber Conditioning Station for Agilent GC Sampler 80 120 Contents Contents 1 General Information 5 Safety Information 6 How to Use this Manual 13 2 Installation of PAL SPME Fiber Conditioning Station 15 General System Overview 16 Installation 18 PAL Firmware and PAL Object Installation for PAL SPME Fiber Conditioning Station Option 22 3 PAL SPME Fiber Conditioning Station Operation 29 Operation and Temperature Setting 30 4 Appendices 33 Definition of Terms 34 Naming Convention 37 Spare Parts 38 SPME Fiber Conditioning Station for Agilent GC Sampler 80 120 3 Contents 4 SPME Fiber Conditioning Station for Agilent GC Sampler 80 120 SPME Fiber Conditioning Station for Agilent GC Sampler 80 120 1 General Information Safety Information 6 How to Use this Manual 13 ee Agilent Technologies 1 General Information Safety Information General Considerations A The SPME Upgrade System U
4. of electrical damage Instead disconnect the power cord and contact a Agilent Technologies representative for a product evaluation Do not attempt to use the instrument until it has been evaluated Electrical damage may have occurred if the SPME Upgrade System shows visible signs of damage exposure to any liquids or has been transported under severe stress Damage can also result if the instrument is stored for prolonged periods under unfavorable conditions e g subjected to heat water etc AN In any case disconnect the power cord s from the power supply or from the different power supplies if optional devices are installed before attempting any type of maintenance Capacitors inside the instrument may still be charged even if the instrument is turned off AN To avoid damaging electrical parts do not disconnect an electrical assembly while power is applied to the SPME Upgrade system Once the power is turned off wait approximately 30 seconds before you disconnect an assembly The instrument includes a number of integrated circuits These circuits may be damaged if exposed to excessive line voltage fluctuations and or power surges A Never try to repair or replace any components of the instrument that is not described in this manual without the assistance of a Agilent Technologies representative There are no operator serviceable or replaceable parts inside the power supply ies or in the SPME Upgrade System If a power s
5. the Agilent GC Injector 80 120 Follow the instructions described in the Agilent GC Injector 80 120 Operating Manual Chapter Object Positioning Path Menu Setup Objects Injector NdlHeatr SPME Fiber Conditioning Station for Agilent GC Sampler 80 120 25 2 Installation of PAL SPME Fiber Conditioning Station Use the rear well with the larger diameter as the teaching point The lower needle guide preferable if a red magnetic ring is installed must be positioned in the rear well of the SPME Fiber Conditioning Station The metallic plate of the injection unit needle guide bridge will be positioned on the knob of the gas valve From this point turn the Z axis 2 0 mm further down The motor current for the injection unit used for teaching is not high enough to press down the spring loaded valve Position the lower needle guide bridge exactly on the knob of the valve Changing the Z axis value manually by 2 0 mm further down then defines the correct position for automated routine The value of Needle Penetr has been optimized it is not necessary to change this value Cycle Composer Control The SPME Fiber Conditioning Station Option can be used in standalone mode or software controlled The SPME Fiber Conditioning Station Option can be operated together with the PAL control software Cycle Composer or the Cycle Editor for ICC interpretation in another data handling software Make sure the hard and software requi
6. 20 SPME Fiber Conditioning Station for Agilent GC Sampler 80 120 4 Appendices Definition of Terms 34 Naming Convention 37 Spare Parts 38 ee Agilent Technologies 33 4 Appendices Definition of Terms 34 Job Queue A Job Queue is a list of sample processing Jobs Jobs are executed in the order displayed on the JOB QUEUE menu screens New Jobs may be added to the queue while samples are being processed Job A Job contains the information needed by the SPME Upgrade to process multiple samples by the same processing steps The elements of a Job are a Method and a Tray that define the location of the samples to be processed For identification Jobs are automatically numbered from 01 to 99 and then restarting with 01 when they are added to the Job Queue Cycle A Cycle consists of the specific operations necessary to process one sample The Cycle operations are repeated for each sample within a Job Cycles are designed for specific applications Method A Method defines how the samples are processed The elements of a Method are a Cycle a Syringe and a Parameter List Methods have names with up to eight characters and can be edited copied and deleted Method Parameters Method Parameters are associated with the Cycle operations User assigned Parameter values define how a processing operation is performed A zero Parameter value will disable a Cycle operation Cycle Parameters are application specific Tray Ho
7. Composer Control 26 Installation of Macros for Cycle Composer 27 BE Agilent Technologies 15 2 Installation of PAL SPME Fiber Conditioning Station General System Overview General System Overview 16 Figure 1 SPME Fiber Conditioning Station The SPME Fiber Conditioning Station is an optional device used for the SPME technique Solid Phase Micro Extraction The purpose is to clean or condition a fiber between chromatographic runs A flow of inert gas protects the fiber from degradation if exposed to elevated temperatures The temperature for conditioning or cleaning is selectable A spare fiber can be conditioned in the front well to ensure a spare fiber is always ready for use SPME Fiber Conditioning Station for Agilent GC Sampler 80 120 Installation of PAL SPME Fiber Conditioning Station 2 Specifications Table 2 Specifications Article Number PAL FibCond Description SPME Fiber Conditioning Station Temperature Control 30 C to 350 C in steps of 1 C Dimensions L 50 mm D 175 mm H 170 mm Std bracket holder H 144 mm Short bracket holder Weight 820 g PAL Hardware Requirements The SPME Fiber Conditioning Station can be used with any PAL System A gas supply has to be provided Consequently a PAL System equipped for headspace or SPME technique would be a logical choice The electrical heating is provided via an Aux interface typically AUX2 Software Requirements The heating for t
8. SPME Fiber Conditioning Station for Agilent GC Injector User Manual Sinj Agilent Technologies Notices Agilent Technologies Inc 2010 No part of this manual may be reproduced in any form or by any means including elec tronic storage and retrieval or translation into a foreign language without prior agree ment and written consent from Agilent Technologies Inc as governed by United States and international copyright laws Manual Part Number 66504 90000 Edition 08 2010 Printed in Germany Agilent Technologies Hewlett Packard Strasse 8 76337 Waldbronn This product may be used as a com ponent of an in vitro diagnostic sys tem if the system is registered with the appropriate authorities and com plies with the relevant regulations Otherwise it is intended only for gen eral laboratory use Warranty The material contained in this docu ment is provided as is and is sub ject to being changed without notice in future editions Further to the max imum extent permitted by applicable law Agilent disclaims all warranties either express or implied with regard to this manual and any information contained herein including but not limited to the implied warranties of merchantability and fitness for a par ticular purpose Agilent shall not be liable for errors or for incidental or consequential damages in connection with the furnishing use or perfor mance of this document or of any information c
9. ary equipment pick up and recycling Lithium battery A An onboard lithium battery buffers the electronic memories when the instrument is turned off Replace it only with the same or equivalent type recommended by the equipment manufacturer Battery Panasonic VL 2330 soldered directly on the electronic board Discharged lithium batteries shall be disposed off locally according to national waste disposal regulations for batteries There are no operator serviceable parts on the electronic boards If an electronic board fails contact a Agilent Technologies representative SPME Fiber Conditioning Station for Agilent GC Sampler 80 120 11 1 General Information Table 1 Commonly Used Symbols Symbol Description Caution or refer to User Manual Caution Risk of Needle Stick Puncture Caution Hot Surface or High Temperature Ibe e Direct Current Alternating Current Protective Conductor Terminal Ground Fuse BLURO Electrical Power ON Used with Main SPME Upgrade Power Supply Electrical Power OFF Used with Main SPME Upgrade Power Supply Electrical Power ON for Only Part of the System Used with Optional Device s Electrical Power OFF for Only Part of the System Used with Optional Device s Caution Risk of Electrical shock high voltage Disposal Do not dispose in municipal waste Follow local waste regulations to reduce electrical and electronic waste WEEE be gt O 12 SPME Fib
10. d for safe operation A To avoid injury observe safe laboratory practice when you handle solvents change tubing or operate the SPME Upgrade System Know the physical and chemical properties of the solvents you use See the Material Safety Sheets from the manufacturer for the solvents in use A Use caution when working with any polymer tubing under pressure Always wear eye protection when near pressurized polymer tubing Do not use polymer tubing that has been severely stressed or kinked Do not use polymer tubing in particular not PEEK or Tefzel tubing with Tetrahydrofuran THF Dimethylsulfoxid DMSO chlorinated organic solvents concentrated mineral acids such as Nitric Phosphoric or Sulfuric acids or any related compounds to above listings A Do not use vials without a sealing cap microtiter or deepwell plates without a plate seal Vapor phase from organic solvents can be hazardous and flammable Acidic vapor phase can cause corrosion to critical mechanical parts SPME Fiber Conditioning Station for Agilent GC Sampler 80 120 General Information 1 Disposal om Do not dispose of this equipment or parts thereof unsorted in municipal waste Follow local municipal waste ordinances for proper disposal provisions to reduce the environmental impact of waste electrical and electronic equipment WEEE European Union customers Call your local customer service representative responsible for the PAL System for compliment
11. ditioning Station Software controlled operation behaves the same way Depending on the macro used the same or similar method variable names are used The automated cycle will go to the conditioning station after the first injection The conditioning station is ignored by the software before the first injection The injection unit needle guide will sit on the spring loaded gas valve At this point gas flow is activated Leaving this position will close the gas valve Gas consumption is reduced to a minimum SPME Fiber Conditioning Station for Agilent GC Sampler 80 120 31 3 32 PAL SPME Fiber Conditioning Station Operation Using the Front Position to Condition a Spare Fiber The front position is designed to condition a spare SPME Fiber The fiber is manually placed into the hole and the fiber is brought to the exposed position If this second position is used open the needle valve for gas flow in the second channel front The needle valve is the metallic knob in front of the conditioning station After the spare fiber has been removed from the front position it is necessary to close the needle valve in front of the conditioning station metallic knob Letting gas flow through this open second channel would reduce the gas flow in the channel for the automated conditioning to a minimum This could result in damage to the fiber as it is not protected by inert gas SPME Fiber Conditioning Station for Agilent GC Sampler 80 1
12. ect Lists to Pal SY Figure 5 PAL Object Manager Send Object PAL SPMEOpt to PAL 24 SPME Fiber Conditioning Station for Agilent GC Sampler 80 120 Installation of PAL SPME Fiber Conditioning Station 2 It is important to understand the meaning of the two Object Lists Table 3 Two object list PAL SPMEOpt This PAL Firmware Object List loads the Objects SPME Fiber and Position FiberExp The two Objects are required to enable the automated SPME technique either in standalone mode from local terminal or by an integration using ICC cycles The Agitator will be used for this SPME cycle SPME Out of Tray This PAL Firmware Object List loads the same for GC PAL Objects but will turn OFF the use of the Agitator Out of Tray means that the fiber goes directly to the selected vial in the Tray not using the temperature stability function from the Agitator Load only the required Object List do not load both types In case the Object List SPME Out of Tray for GC PAL has been added by mistake add the PAL Firmware Object List for the Incubator Agitator to the PAL System With this step the Agitator is again activated For details see also the dedicated User Manual for SPME Technique which is part of the SPME Option Kit The PAL SPME Fiber Conditioning Station has the PAL Firmware Object name NdlHeater and is located in the class Injectors The Object NdiHeater must be positioned in
13. er 80 120 See Figure 2 on page 20 AUX 2 INTERFACE 1 INTERFACE Ke o o 2 Figure 2 SPME Fiber Conditioning Station Electrical and Gas Connections 2 Power up the System again With this warm start the system again recognizes and references the module connected to AUX Interface 20 SPME Fiber Conditioning Station for Agilent GC Sampler 80 120 Installation of PAL SPME Fiber Conditioning Station 2 Gas Line Connections One end of the supplied black gas tube is connected to the flush gas regulator supplied with the Combi PAL see Chapter Installation in the Agilent GC Sampler 80 120 manual 1 Remove the blind plug at the gas regulator and connect the corresponding reducing union supplied with the installation kit The other side of the gas tube is connected with a 1 8 inch Swagelok nut to the Fiber Conditioning Station The installation of this second gas line is displayed in Figure 3 on page 21 Figure 3 Gas Tube Connection from Pressure Regulator to SPME Fiber Conditioning Station SPME Fiber Conditioning Station for Agilent GC Sampler 80 120 21 2 Installation of PAL SPME Fiber Conditioning Station PAL Firmware and PAL Object Installation for PAL SPME Fiber Conditioning Station Option 22 PAL Firmware Installation for SPME Fiber Condit
14. er Conditioning Station for Agilent GC Sampler 80 120 General Information 1 How to Use this Manual The major sections of this Addendum are Safety Information e SPME Fiber Conditioning Station Installation e SPME Fiber Conditioning Station Operation e Appendices This Addendum is intended for frequent or new PAL System users who are experienced at using automated systems to run existing analytical methods The Appendices provide information on PAL SPME Fiber Conditioning Station and Spare Parts The Agilent GC Sampler 80 120 must be installed and set up properly before the SPME Fiber Conditioning Station Operating instructions can be used SPME Fiber Conditioning Station for Agilent GC Sampler 80 120 13 1 General Information How to Use this Manual 14 SPME Fiber Conditioning Station for Agilent GC Sampler 80 120 SPME Fiber Conditioning Station for Agilent GC Sampler 80 120 2 Installation of PAL SPME Fiber Conditioning Station General System Overview 16 Specifications 17 PAL Hardware Requirements 17 Software Requirements 17 Installation 18 Unpacking the Components 18 Assembling the SPME Fiber Conditioning Station 19 Electrical Connections 20 Gas Line Connections 21 PAL Firmware and PAL Object Installation for PAL SPME Fiber Conditioning Station Option 22 PAL Firmware Installation for SPME Fiber Conditioning Station 22 PAL Firmware Object List Installation for the SPME Fiber Conditioning Station 23 Cycle
15. es of the type and current rating specified Do not use repaired fuses and do not short circuit the fuse holder A The supplied power cord must be inserted into a power outlet with a protective earth contact ground When using an extension cord make sure that the cord also has an earth contact A Do not change the external or internal grounding connections Tampering with or disconnecting these connections could endanger you and or damage the SPME Upgrade System The instrument is properly grounded in accordance with these regulations when shipped You do not need to make any changes to the electrical connections or the instrument s chassis to ensure safe operation A The combination of a SPME Upgrade System with a LC MS System does require the safety measure as described by the LC MS System manufacturer Detailed instructions for the safety grounding on the LC MS system are outlined in the corresponding operating installation manual Agilent Technologies recommends to use a grounding cable connected on one side at the Injection Valve Loop or any other suitable direct metallic contact and the other side at an appropriate grounding point at the LC MS System This supplementary grounding measure will support the safety strategy of the LC MS System manufacturer SPME Fiber Conditioning Station for Agilent GC Sampler 80 120 7 1 General Information AN Do not turn the instrument on if you suspect that it has incurred any kind
16. he SPME Fiber Conditioning Station is controlled directly by the PAL System Temperature control via software such as Cycle Composer or certain data handling systems that control the PAL using Cycle Editor for PAL ICC interpretation is not possible The SPME Fiber Conditioning Station is compatible with PAL Firmware level 2 0X or higher However the minimum firmware level for the SPME technique should be 2 2 7 or higher SPME Fiber Conditioning Station for Agilent GC Sampler 80 120 17 2 Installation of PAL SPME Fiber Conditioning Station Installation Unpacking the Components The SPME Fiber Conditioning Station is shipped in one box Check for the following items e SPME Fiber Conditioning Station e PAL SPME Fiber Conditioning Station Gas tube Teflon black Swagelok Union 1 8 inch includes flow reduction Reducing Unit M5 PAL Zub SPME Short Bracket 14 mm with 2 screws and 2 serrated washers Disk PAL Object Manager Software Software to add new Objects to the Agilent GC Sampler 80 120 18 SPME Fiber Conditioning Station for Agilent GC Sampler 80 120 Installation of PAL SPME Fiber Conditioning Station 2 Assembling the SPME Fiber Conditioning Station Before beginning the assembly process determine approximately where the SPME Fiber Conditioning Station will be located Consider the height of the station in order to be able to move a 20 ml vial across from the Tray to the Agitator and visa versa If there is not eno
17. ioning Station The PAL SPME Fiber Conditioning Station option requires PAL Firmware level 2 0 X or higher If a lower firmware level is installed on the unit in use an upgrade of the firmware to the current level is recommended However the minimum firmware level for the SPME technique should be 2 2 7 or higher To backup the configuration see the Agilent GC Sampler 80 120 manual SPME Fiber Conditioning Station for Agilent GC Sampler 80 120 Installation of PAL SPME Fiber Conditioning Station 2 PAL Firmware and PAL Object Installation for PAL SPME Fiber Conditioning Station Option PAL Firmware Object List Installation for the SPME Fiber Conditioning Station If the revision level of the PAL Object Manager List installed on the computer is lower than K copy the provided folder PAL SPME Fiber Conditioning Station from the CD ROM to the Object List folder which has been installed with the Object Manager This software is usually installed in the following path C Program Files PAL Object Manager Object Lists 1 Start Object Manager The PAL SPME Fiber Conditioning Station folder should be visible in the structure shown in the window Choose Object List Folder on the left side of the Object Manager program window C Program Files PAL Object Fiber Conditioning Stations Choose Object List Folder Object Lists d Flow Cells PAL FibCond_Aux1 pr GC Injectors PAL FibCond_Aux2 f Incubators D ITEX 2 j ITEX Option
18. lder A Tray Holder can hold one or more trays Each Tray Holder has a reference position X Y Z coordinates that defines its location SPME Fiber Conditioning Station for Agilent GC Sampler 80 120 Appendices 4 Tray A Tray holds multiple samples Trays are defined by designating the Tray Type see below and the Tray Holder Tray names are used to identify the sample source within a Job Tray Type A Tray Type defines the pattern and sampling sequence of sample locations within a Tray Stack A Stack is a particular type of Tray Holder that is designed to hold micro plates A six drawer Stack holds 12 standard microplates two in each drawer A three drawer Stack holds six deep well micro plates two in each drawer Object Manager Software to load an Object List to an instrument if a Module hardware module has been added to the System In a special mode Object Manager can also be used to create and maintain Object Lists Object List If a Module hardware is added to an instrument several Objects have to be loaded into the firmware These Objects are collected in an Object List and stored in a file with the extension pol Object Lists are delivered together with Object Manager Software and are grouped into folders for the different kind of Modules e g Syringes Tray Holders Valve Drives The name of an Object List starts with the Module part number with variants added e g first or second Stack The na
19. me of the root folder includes the revision which is dependent on the firmware version e g Object Lists Rev K for firmware 2 X and 3 X Object Class Each Object belongs to an Object Class e g Syringes Trays Injectors The Object Class defines the Items of an Object SPME Fiber Conditioning Station for Agilent GC Sampler 80 120 35 4 36 Appendices Object Item An Object contains several Items which can be numerical values with a physical unit e g X Y Z Position Penetration Syringe Scale Length Syringe Volume or references to other objects The term Parameter is reserved for ATOM Parameter Firmware commands to be used for a Cycle Objects Objects are data structures describing the properties of physical modules Certain modules e g a Stack require several objects Module Hardware module either part of a standard system configuration e g Agilent LC Injectors HTC HTS Agilent GC Sampler 80 120 or an optional addition e g SPME Upgrade for Agilent GC Injector MT DW Tray Stack and Cooler Upgrades for Agilent LC Injectors HTC HTS The term Module is intentionally used to differentiate from Object which is reserved for the Firmware Object SPME Fiber Conditioning Station for Agilent GC Sampler 80 120 Naming Convention Naming Convention This section recommends standard naming convention for SPME Upgrade Trays Tray Types and Tray holders Following these conven
20. ontained herein Should Agilent and the user have a separate written agreement with warranty terms covering the material in this document that conflict with these terms the warranty terms in the sep arate agreement shall control Technology Licenses The hardware and or software described in this document are furnished under a license and may be used or copied only in accor dance with the terms of such license Restricted Rights Legend If software is for use in the performance of a U S Government prime contract or subcon tract Software is delivered and licensed as Commercial computer software as defined in DFAR 252 227 7014 June 1995 or as a commercial item as defined in FAR 2 101 a or as Restricted computer soft ware as defined in FAR 52 227 19 June 1987 or any equivalent agency regulation or contract clause Use duplication or dis closure of Software is subject to Agilent Technologies standard commercial license terms and non DOD Departments and Agencies of the U S Government will receive no greater than Restricted Rights as defined in FAR 52 227 19 c 1 2 June 1987 U S Government users will receive no greater than Limited Rights as defined in FAR 52 227 14 June 1987 or DFAR 252 227 7015 b 2 November 1995 as applicable in any technical data Safety Notices CAUTION A CAUTION notice denotes a hazard It calls attention to an operating procedure practice or the like that
21. rements meet those of your installation before operating the SPME Fiber Conditioning Station See PAL Hardware Requirements on page 17 and Software Requirements on page 17 26 SPME Fiber Conditioning Station for Agilent GC Sampler 80 120 Installation of PAL SPME Fiber Conditioning Station 2 Installation of Macros for Cycle Composer In order to use the SPME Fiber Conditioning Station in an automated manner for the SPME technique no dedicated Cycle Composer macro is required The conditioning station is only a tool to clean the fiber The SPME method either as used from the local terminal standalone or software controlled such as by Cycle Composer makes use of the conditioning station if required by the application The activation of the use is a SPME method parameter selectable by the user If the SPME technique is to be used via software control Cycle Composer or certain data handling systems that control the PAL using Cycle Editor for PAL interpretation then it will be necessary to load the corresponding macros for the SPME technique not for the conditioning station as such In case the SPME macro s has have not been added yet install the software as described in the PAL Cycle Composer Software Manual and add the SPME macros located in the Combi PAL folder The provided macros for SPME are installed with this option To verify this step navigate in Explorer to the Cycle Composer folder This is usuall
22. ser Manual and the corresponding Addendum for a specific module must be consulted by the user under all circumstances before a unit is put in use Changes or modifications to this unit not expressly approved by the party responsible for compliance could void the user s authority to operate the equipment The user shall be made aware that if the equipment is used in a manner not specified by the manufacturer the protection provided by the equipment may be impaired When using the SPME Upgrade System follow the generally accepted procedures for quality control and methods development When you use the SPME Upgrade System in the field of chromatographic analysis and you observe a change in the retention of a particular compound in the resolution between two compounds or in peak shape immediately determine the reason for the changes Until you determine the cause of a change do not rely on the separation results SPME Fiber Conditioning Station for Agilent GC Sampler 80 120 General Information 1 Electrical Every analytical instrument has specific hazards so be sure to read and Hazards comply with the following precautions They will help ensure the safe long term use of your SPME Upgrade System The Installation Category Over voltage Category for this instrument is Level II The Level II Category pertains to equipment that receives its electrical power from the local level such as an electrical wall outlet Only use fus
23. st the SPME Application CD SPME Fiber Conditioning Station for Agilent GC Sampler 80 120 Index Index A assembling 19 Cc components 18 condition spare fiber 32 connections gas line 21 control cycle composer 26 E electrical connections 20 F firmware installation 22 G gas flow setting 30 M macros installation 27 N naming convention 37 0 object list installation 23 R requirements hardware 17 software 17 S safety information 6 spare parts 38 specifications 17 system overview 16 T temperature setting 30 terms definition 34 SPME Fiber Conditioning Station for Agilent GC Sampler 80 120 39 www agilent com In This Book This manual contains technical reference information about the SPME Fiber Conditioning Station for Agilent GC Injector The manual describes the following e safety information how to use this manual installation operation Agilent Technologies 2010 Printed in Germany 08 2010 G6504 90000 ee Agilent Technologies
24. tions will allow SPME Upgrade to be pre configured for certain applications will simplify software backups and application development and will improve technical support and training Table4 Naming Convention Tray Type Tray Description VT200 Vial Tray 200 positions 10 x 20 For 7 mm micro vials 1 ml VT98 Vial Trays 98 positions 7 x 14 For 12 mm vials 2 ml VT78 Vial Tray 78 positions 6 x 13 For 7 mm micro vials 1 ml opposite side of 98 positions Tray VT54 Vial Tray 54 positions 6 x 9 For 12 mm vials 2 ml VT21 Vial Tray 21 Positions 7 x 14 For 12 mm vials 2 ml opposite side of 32 positions Tray VT32 10 Vial Tray 32 positions 4 x 8 For 23 mm headspace vials 10 ml VT32 20 Vial Tray 32 positions 4 x 8 For 23 mm headspace vials 20 ml MT96 Standard 96 position shallow microplate DW96 Deep well 96 position microplate MT384 High density 384 position shallow microplate SPME Fiber Conditioning Station for Agilent GC Sampler 80 120 37 4 Appendices Spare Parts 38 Table5 Spare parts Part Number Description G6500 88003 Gas tube fibCond to Pressure regulator incl swageLok fittings and flow restrictor Fibers for the SPME technique are solely sold by Supelco which is today part of Sigma Aldrich For details see following web page www sigmaaldrich com Application notes for the SPME technique are also provided by Supelco Check the web page www sigmaaldrich com spme to reque
25. ugh space between the X axis of the Agilent GC Sampler 80 120 and the GC top cover consider installing the short bracket which is part of the installation kit If the SPME Fiber Conditioning Station is to be installed with an existing Agilent GC Sampler 80 120 System another hardware module may need to be shifted Remember to re position the modules again according the Agilent GC Sampler 80 120 manual 1 Loosen the Torx screw on the mounting clamps located on the top side of the Conditioning Station 2 Be sure that the clamps fit entirely into the grooves Tighten the Torx screw until the mounting clamps are firmly in place 3 Double check whether the SPME Fiber Conditioning Station clamp is correctly attached to the X crossrail see Agilent GC Sampler 80 120 manual The short bracket that is provided is a spacer 14 mm high which could be installed instead of the standard bracket in cases where the total height of the fiber conditioning station does not physically fit the System SPME Fiber Conditioning Station for Agilent GC Sampler 80 120 19 2 Installation of PAL SPME Fiber Conditioning Station Electrical Connections Installing the SPME Fiber Conditioning Station It is important to power off the Agilent GC Sampler 80 120 System before the SPME Fiber Conditioning Station is electrically connected 1 Connect the open end of the cable from the SPME Fiber Conditioning Station to the AUX2 connector on the Agilent GC Sampl
26. upply is not functioning contact a Agilent Technologies representative SPME Fiber Conditioning Station for Agilent GC Sampler 80 120 General Information 1 A The power supply for the SPME Upgrade Instrument has the symbols 1 0 on the label for the power switch to switch ON OFF Any additional power supply for other devices like Cooled Stack or a Valve Module shows the symbols as shown below on the label for the power switch O The symbols shall warn the user that in a emergency case more than one power supply has to be turned OFF or more than one power cord has to be pulled from power supply or from the wall outlet to shut down the complete SPME Upgrade System If the basic SPME Upgrade System is installed than a single power supply is installed only Turning OFF the power supply or pulling this single power cord in an emergency case will stop the complete SPME Upgrade System It is important that the power supply ies are in a location where the power ON and OFF switch is accessible and easy to operate and where it is possible to unplug the AC power cord from the power supply wall outlet in case of emergency SPME Fiber Conditioning Station for Agilent GC Sampler 80 120 9 1 10 General Information Other Hazards A To avoid injury during SPME Upgrade System operation keep your hands away from the syringe A Do not operate the SPME Upgrade System without the safety shield The safety shield must be installe
27. y installed in the following path C Program Files PAL Cycle Composer SPME Fiber Conditioning Station for Agilent GC Sampler 80 120 27 2 Installation of PAL SPME Fiber Conditioning Station PAL Firmware and PAL Object Installation for PAL SPME Fiber Conditioning Station Option 28 SPME Fiber Conditioning Station for Agilent GC Sampler 80 120 SPME Fiber Conditioning Station for Agilent GC Sampler 80 120 3 PAL SPME Fiber Conditioning Station Operation Operation and Temperature Setting 30 Temperature Setting 30 Gas Flow Setting 30 SPME Fiber Conditioning Station Operation 31 Using the Front Position to Condition a Spare Fiber 32 Agilent Technologies 29 3 PAL SPME Fiber Conditioning Station Operation Operation and Temperature Setting Temperature Setting The SPME Fiber Conditioning Station is controlled directly by the Agilent GC Sampler 80 120 firmware Use the Utilities section to set the desired temperature for fiber conditioning Standby Temp Path Menu Utilities Injector NdIHeatr Gas Flow Setting The gas flow is regulated by the Flush Gas Pressure Regulator The setting of the secondary regulator is the same as for Headspace Analysis approximately 0 5 bar The Fiber Conditioning Station has an internal restriction built in The typical flow rate yields approx 6 ml min if only the rear position is used If both positions are used front and rear the flow is divided approximately in half
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