Typhoon™ FLA 9000 - GE Healthcare Life Sciences
Contents
1. My Documents Y My Compter lt a Loca Dik C s gt STORE NGO L Sheed Docurnerts O My Documents 12 13 Look je C3 USB Control 14 Click the OK button in the Install from disk dialog 15 Click the Next button in the Add hardware wizard 16 Click the Next button again 28 Getting Started with Typhoon FLA 9000 28 9607 66 AB Step 3 Installation 3 5 Software installation 3 5 1 Install Typhoon FLA 9000 Control Software under Windows XP Action 17 18 Click the Continue Anyway button Hardware Installation The software you are installing for this hardware Fujifilm USB Device Managment Driver has not passed Windows Logo testing to verify its compatibility with Windows XP Tell me why this testing is important Continuing your installation of this software may impair or destabilize the correct operation of your system either immediately or in the future Microsoft strongly recommends that you stop this installation now and contact the hardware vendor for software that has passed Windows Logo testing Continue Anyway STOP Installation Click the Finish button to complete the driver installation Install the USB function driver Windows XP Step Action 1 4 Connect the computer and the Typhoon FLA 9000 with a USB cable and turn ON the power switch of Typhoon FLA 9000 The scanner is automatically detected by the com puter In the Found New Hardware Wizard dialog cho2. Radiation safety This instrument is not equipped with any radioisotope RI or radiation generating unit and is therefore not regulated by radiation hazard prevention laws However the instrument is capable of reading Storage phosphor screens which may be polluted by radioisotopes RI CAUTION If radioisotope RI pollution occurs stop use of the instrument immediately and follow the instructions of your radiation administrator Personal protection CAUTION Always wear gloves protective glasses and a lab coat or similar when handling samples CAUTION Always wear cotton gloves when handling storage phosphor screens Getting Started with Typhoon FLA 9000 28 9607 66 AB 17 2 Safety instructions 2 1 Safety precautions Installing and moving the instrument WARNING The Typhoon FLA 9000 instrument must always be connected to a grounded power outlet WARNING Do not block the ventilation inlets or outlets on the system WARNING HEAVY OBJECT Use suitable lifting equipment when moving the unit gt gt gt gt WARNING Power cord Only use power cords delivered or approved by GE Healthcare System operation WARNING Do not use the equipment with a power supply other than that recommended Fire and electric shock could result WARNING Do not use the equipment within or near a sink or in humid or dusty environments Fire and
3. Reagent name Excitation wavelength Emission light wavelength Filter DY 682 690 709 BPFR700 IRDye 680 683 710 BPFR700 IRDye 700 689 700 BPFR700 Krypton Infrared 690 718 BPFR700 785 nm laser Reagentname Excitation wavelength Emission light wavelength Filter Alexa Fluor 750 746 775 BPFR800 Alexa Fluor 790 784 814 BPFR800 DY 781 783 800 BPFR800 IRDye 800 778 806 BPFR800 Getting Started with Typhoon FLA 9000 28 9607 66 AB 73 E Specifications Appendix E Specifications Scanning specifications Parameter Data Scanned image size 40 x 46 cm Pixel size 10 25 50 100 or 200 um and a 1000 um prescan Gradation bit depth 16 bit Dynamic range Five orders of magnitude Image capacity e 3510 MB 10 um e 561 52 MB 25 um e 140 35 MB 50 um e 35 09 MB 100 um e 8 77 MB 200 um Detection sensitivity e Storage phosphor screen C Detectable to 0 9 dom mm e Fluorescent DNA SYBR Green 7 pg band e Enzyme multiplied fluorescence sensitivity pBR328 AttoPhos 100 fg spot Outside dimensions amp weight Dimensions amp weight Unit Dimension mm Weight kg wxdxh Reading block 900 x 800 x 400 97 projections not includ ed Storage phosphor screen cassette 460 x 430 x 20 Approx 2 5 Phosphor stage 502 x 538 x45 Approx 2 5 Multi stage 502 x 538 x45 Approx 2 8 Fluor stage 502 x 538 x45 Approx 2 3
4. 1 Introduction 1 3 The Typhoon FLA 9000 laser scanner Glass slide holder Suction rod Digitization plate Membrane weight 1 4 Typhoon FLA 9000 Control Software Overview of the main window The Typhoon FLA 9000 Control Software is used to control use and supervise the Typhoon FLA 9000 he Typhoon FLA 9000 CONTROL SOFTWARE imagination at work A 12 Getting Started with Typhoon FLA 9000 28 9607 66 AB 1 Introduction 1 4 Typhoon FLA 9000 Control Software Part Function 1 Phosphorimaging button click to read a storage phosphor screen N Fluorescence button click to read a fluorescent sample Chemiluminescence button click to read a chemiluminescent sample FW Digitization button click to perform digitization nn Method selection button click to register or erase a combination of laser and filter Filter module click to change or register a filter Preferences click to select file format and correction mode Filter displays the loaded filters Oo OO N Dv Laser displays the status of the loaded laser units PMT displays the status of the loaded photo multiplier tube Overview of the reader settings window 8 9 Fa tat no Ts Condition File Save Condition Load 2 image Folder proye rete remmer r rros FLA 10 Oe 2 bere File Name _ ae 7 jamaa 4 w Lent J lt
5. FLA Image Eraser 603 x 512 x 164 14 5 74 Getting Started with Typhoon FLA 9000 28 9607 66 AB Power supply E Specifications Parameter Data Input voltage 100 to 240V AC single phase Allowable variations in volt 10 age Frequency 50 to 60 Hz Rated current 1 5 to 3 0 A Environmental conditions Parameter Data Operating conditions Temperature 15 C to 30 C Humidity 20 to 75 RH no dew condensation Non operating conditions Temperature 10 C to 40 C Humidity 20 to 70 RH no dew condensation Transportation amp storage temperature Temperature 25 C to 70 C Humidity 10 to 80 no dew condensation Heat radiation 151 W h reader block FLA Image eraser Lighting It is recommended to lower the lighting level to about 20 lux when moving a sample from the cassette into Typhoon FLA 9000 after exposure Where to use Indoor use only Maximum altitude for use 2 000 m above sea level Overvoltage category Transient overvoltage category II Applicable rated pollution degree Pollution Degree 2 Getting Started with Typhoon FLA 9000 28 9607 66 AB For local office contact information visit www gelifesciences com contact GE Healthcare Bio Sciences AB Bj rkgatan 30 751 84 Uppsala Sweden www gelifesciences com quantitative_imaging imagination at work GE imagination at work an
6. CE Conformity This product complies with the European directives listed in the table by fulfilling the corresponding harmonized standards A copy of the Declaration of Conformity is available on request Directive 2006 42 EC Machinery Directive MD Getting Started with Typhoon FLA 9000 28 9607 66 AB 7 1 Introduction 1 2 Regulatory information Directive Title 2006 95 EC Low Voltage Directive LVD 2004 108 EC ElectroMagnetic Compatibility EMC Directive CE Marking The CE marking and the corresponding Declaration of Conformity is valid for the instrument when it IS e used asa stand alone unit or e connected to other CE marked GE Healthcare instruments or e connected to other products recommended or described in the user documentation and e used in the same state as it was delivered from GE Healthcare except for alterations described in the user documentation Note Note Laser standards This equipment has been tested and found to comply with the limits for a Class A digital device pursuant to Part 15 of the FCC Rules These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a commercial environment This equipment generates uses and can radiate radio frequency energy and if not installed and used in accordance with the instruction manual may cause harmful interference to radio communications Operation of this
7. Control Software under Windows XP Install the USB Control Driver Windows XP Step Action 1 2 3 2 10 Disconnect Typhoon FLA 9000 from the computer Open the control panel and select Printers and Other Hardware Click Add Hardware Click the Next button in the Add hardware wizard Select Yes have already connected the hardware and click the Next button Select Add a new hardware device and click the Next button From the tat below select an intatied hardware device then click Nest to chech popeta a boubleshoot a protiem you might be having To add hademe not hoen n the kat click Add a now hardware device Select Install the hardware that manually select from a list Advanced and click the Next button Select Show All Devices and click the Next button From the bit beirm select the type al hardware pou are mstaliong If you do not see the hademe Category you mart cick Show All Devices Common hardewe typer N Ccckay adapters Fetes USB Device RICE ATAATA corisslers CLL 1204 Da hon cormaters Click the Have Disk button Insert the Typhoon FLA 9000 Control Software CD and click the Browse button Getting Started with Typhoon FLA 9000 28 9607 66 AB 27 3 Installation 3 5 Software installation 3 5 1 Install Typhoon FLA 9000 Control Software under Windows XP Step Action 11 Select to install the driver from the Typhoon FLA 9000 Control Software CD My Flecert Documerts
8. Note Keep the radio imaging sample away from the edge of the storage phos phor screen Otherwise the recorded data may become corrupted 5 Place the storage phosphor screen in the cassette with the exposure surface of the storage phosphor screen facing the sample Make sure that the notch of the storage phosphor screen is in the front left corner of the cassette as shown below 6 Close the cover of the cassette Press on the cover until it clicks into place Getting Started with Typhoon FLA 9000 28 9607 66 AB 53 8 Reading phosphorimaging samples 8 Reading phosphorimaging samples 8 1 Set the reading conditions Start the system 1 Turn on Typhoon FLA 9000 and the computer 2 Start the Typhoon FLA 9000 Control Software Set the reading conditions Click the Phosphorimaging button to reach the Reader Settings window for the phosphorimaging mode Perform the tasks specified in the numbered table below Note All parts in the window are described in Section 1 4 Typhoon FLA 9000 Control Software on page 12 8 9 1 2 3 4 5 6 7 Rocke Veal RAAR AS E Start Scan Part Description 1 Image folder specify where to save the file after the reading 2 File Name enter the name of a file to save image data 54 Getting Started with Typhoon FLA 9000 28 9607 66 AB 8 Reading phosphorimaging samples 8 1 Set the reading conditions Part Description 3 Comment enter an optional comment The comment is em
9. electric shock could result gt gt gt WARNING Connect the power supply directly to a grounded wall power outlet The use of extension cords or multiple loads on one electrical outlet could result in fire and electric shock Getting Started with Typhoon FLA 9000 28 9607 66 AB 2 Safety instructions 2 1 Safety precautions CAUTION Do not use the same power supply as that of large equipment such as an air conditioner or centrifuge Malfunction could result gt NOTICE Avoid exposing storage phosphor screens in places where the environmental radiation may be increased for example rooms with concrete walls or in basements O Maintenance WARNING Do not attempt to modify the equipment or fire and electric shock could result CAUTION Take care when connecting the power supply cable Do not tug on the cable and do not handle the connection plugs with wet hands CAUTION Connect the computer hardware on the same power circuit otherwise the equipment may be influenced by electrical nosie CAUTION Turn off the power switch and remove connecting cables before moving the equipment CAUTION Turn the power switch off before cleaning the inside of the equipment CAUTION Unplug the equipment if it will not be used for an extended period gt e e eo gt Getting Started with Typhoon FLA 9000 28 9607 66 AB 19 2 Safety instru
10. in any clinical procedures or for diagnostic purposes Getting Started with Typhoon FLA 9000 28 9607 66 AB 5 1 Introduction 1 1 Important user information Safety notices This user documentation contains WARNINGS CAUTIONS and NOTICES concerning the safe use of Typhoon FLA 9000 See definitions below Warnings WARNING WARNING indicates a hazardous situation which if not avoided could result in death or serious injury It is important not to proceed until all stated conditions are met and clearly understood Cautions CAUTION CAUTION indicates a hazardous situation which if not avoided could result in minor or moderate injury It is important not to proceed until all stated conditions are met and clearly understood Notices NOTICE NOTICE indicates instructions that must be followed to avoid damage to the product or other equipment Notes and tips Note A Note is used to indicate information that is important for trouble free and optimal use of the product TIP A tip contains useful information that can improve or optimize your procedures Typographical conventions Software items are identified in the text by bold italic text A colon separates menu levels thus File Open refers to the Open command in the File menu Hardware items are identified in the text by bold text e g Power switch 1 2 Regulatory information This section lists the directives and standards
11. installing operating or maintaining the equipment you must be aware of the hazards described in the user documentation Follow the instructions provided to avoid personal injury or damage to the equipment General precautions WARNING Do not use the equipment if smoke strange noises or strange odors can be perceived or if the equipment becomes unusually hot This may result in fire or electric shock Stop using immediately turn off the power switch and unplug the equipment from the power outlet Contact your local GE Healthcare representative to request repair WARNING Do not damage the power supply cord by bending twisting heating or allowing them to become pinned under the equipment Using damaged power cords could result in fire or electric shock If the power supply cords are damaged contact your local GE Healthcare representative for replacements WARNING Do not place the equipment on unstable tables or on inclined surfaces as the equipment could be dropped or fall resulting in injury 16 Getting Started with Typhoon FLA 9000 28 9607 66 AB 2 Safety instructions 2 1 Safety precautions Laser safety WARNING Never detach the inner cover screwed to this instrument If it is detached laser beam may leak with a risk of loss of vision CAUTION Using procedures or adjustments other than those specified in this manual may result in hazardous exposure to laser radiation
12. the side of the phosphor stage Getting Started with Typhoon FLA 9000 28 9607 66 AB 55 8 Reading phosphorimaging samples 8 2 Place the storage phosphor screen on the phosphor stage Step Action s 4 Pick up the storage phosphor screen and move it to the phosphor stage E J e Press one end of the suction rod against the storage phosphor screen then place your finger on the other end and lift the suction rod e Release the storage phosphor screen by releasing your finger from the suction rod Place the storage phosphor screen on the back of the phosphor stage with the white or blue reading surface of the storage phosphor screen facing up 8 3 Place the phosphor stage in the Typhoon FLA 9000 Step Action 1 2 Open the lid of the Typhoon FLA 9000 Position the phosphor stage with the storage phosphor screen face down Push the phosphor stage inwards until it stops Close the lid of the Typhoon FLA 9000 Getting Started with Typhoon FLA 9000 28 9607 66 AB 8 Reading phosphorimaging samples 8 4 Read the sample 8 4 Read the sample Click the Start Scan button to start reading Note If you click the Stop button during reading the part that has not been read yet will be saved as an image with a data value of 0 light intensity of 0 Note When you click the Stop button the reading is aborted You cannot start reading again from the location where reading stopped Adjust the d
13. v 280 1000 E r19 pm _ _ Area Fluorescence Stage sre z 5 am a E u 7 Foi r ma 38 ras Eo 6 10 File Format Gel Getting Started with Typhoon FLA 9000 28 9607 66 AB 5 Reading fluorescent samples 5 1 Set the reading mode and reading conditions Part Description 1 Image folder specify where to save the file after the reading 2 File Name enter the name of a file to save image data 3 Comment enter an optional comment The comment is embedded in the file where the image is saved and can be viewed with the analyzing software 4 Method set the method to use in the scan Up to 4 scans can be performed in a row all with individual methods 5 PMT set the voltage of the photo multiplier tube The higher the value the higher the sensitivity Click to increase the number of scans Click to decrease the number of scans 6 Set the scanning area 1 Select a method in the drop down menu 2 Drag the red square to the desired position of the scanning area 3 Drag the sides of the red square as needed to adjust the size of the scanning area 7 Pixel Size set the pixel size Choose a small pixel size for high quality images Note that a small pixel size setting increases the reading time and the size of the image file 8 Save Condition click this button to save the current reading conditions in a file if desired For details refer
14. 66 AB Parameter Explanation Color 6 Reading chemiluminescent samples Select the colors used to display the data Value Color of low Color of high values values Negative Gray White Black Positive Gray Black White Red Black Red Green Black Green Blue Black Blue Color32 Blue Red Getting Started with Typhoon FLA 9000 28 9607 66 AB 6 4 Read the sample 49 7 Exposing storage phosphor screens 7 Exposing storage phosphor screens 7 1 Handling precautions Check the exposure environment NOTICE Avoid exposing storage phosphor screens in places where the environmental radiation may be increased for example rooms with concrete walls or in basements Do not stack cassettes during exposure Wear gloves CAUTION Always wear cotton gloves when handling storage phosphor screens Use a suction rod when taking the storage phosphor screen out of the cassette Prying the storage phosphor out may result in the edge peeling off making the storage phosphor screen unusable Handle the storage phosphor screen carefully e Do not scratch or bend the storage phosphor screen e Keep the storage phosphor screen free from dust Protect the storage phosphor screen from water and volatile solvents e Dry the sample thoroughly before exposing it e Wrap wet samples in plastic film and make sure that no liquid exits Use double layers if the sample contains volatile solvents e f w
15. 8 LPB FAM 490 520 LPB FITC 494 520 LPB DBR1 Flamingo 512 535 LPB Pro Q Emerald488 510 520 LPB Qdot 605 603 LPG Qdot 655 655 LPR Qdot 705 702 LPR Qdot 800 792 LPR SYBR Gold 495 537 LPB SYBR Green 494 521 LPB SYBR Green II 492 513 LPB SYBR Safe 502 530 LPB SYPRO Orange 472 570 LPB SYPRO Ruby 450 610 LPG SYPRO Orange 490 640 LPG Getting Started with Typhoon FLA 9000 28 9607 66 AB 71 D Default sample detection methods 532 nm laser Reagent name Excitation wavelength Emission light wavelength Filter Alexa Fluor 53X 532 554 LPG Alexa Fluor 555 553 568 LPG Cy3 550 570 LPG DGR1 BPG1 Deep Purple 528 591 LPG DY 520XL 520 664 LPR EtBR 518 605 LPG HEX 535 553 LPG HNPP 550 562 LPG Pro Q Diamond 555 580 LPG RITC 554 577 LPG ROX 535 567 LPG SYPRO Red 547 631 LPR TAMRA 542 568 LPG 635 nm laser Reagent name Excitation wavelength Emission light wavelength Filter Alexa Fluor 647 653 669 LPR Cy5 649 670 LPR DBR1 DGR1 DDAO Phosphate 634 665 LPR DY 635 647 671 LPR 685 nm laser Reagent name Excitation wavelength Emission light wavelength Filter Alexa Fluor 680 679 702 BPFR700 Alexa Fluor 700 689 700 BPFR700 Cy5 5 675 694 BPFR700 DY 676 674 699 BPFR700 72 Getting Started with Typhoon FLA 9000 28 9607 66 AB D Default sample detection methods
16. FLA 1000 02 O0 Type Daremot Loutan Rnd ee lt Cr Getting Started with Typhoon FLA 9000 28 9607 66 AB 31 3 Installation 3 5 Software installation 3 5 2 Install Typhoon FLA 9000 Control Software under Windows Vista Step Action 11 12 13 14 15 16 Select the file DevMng and click the Open button lee fae mn USE Cron of 7m So aun E amp a Compas F Manes rene a Ca Click the OK button in the dialog Install from disk Click the Next button in the wizard Add hardware Click the Next button once again Click Install this driver software anyway E Windows Securty OOO n E Click the Finish button to complete the installation Install the USB function driver Windows Vista Note Step During software installations you may be asked to confirm your actions in a dialog with the text Windows needs your permission to continue Enter an administrator password if prompted then click Continue to proceed with the installation Action 32 Connect the computer and the Typhoon FLA 9000 with a USB cable and turn ON the power switch of Typhoon FLA 9000 Result The scanner will automatically be detected by the computer and the Plug and Play function in Windows Vista starts In the Found New Hardware dialog select Locate and install driver software recom mended Insert the Typhoon FLA 9000 Control Software CD and click the Next button in the Found New Hardwa
17. GE Healthcare Getting Started with Typhoon FLA 9000 Original instructions Table of Contents DRM ec cecocctgeesscce EE eset 1 1 Important user INFOPMATION uu ssssesssssssscseessseessssesceceesssssnseeeseeeseessnnnenesssseseseseenssnseseseeeeeeesenee LZ Regulatory IMPS TIGU ON cixcaieauienieniciteseneGranierneidiauane natal inan LS THE Typhoon FLA 9000 laser scanner ssasesecassesdssvevecscsesneciiasexdoviaertapscssslonsvevdosssnonsaettaconvesse 1 4 Typhoon FLA 9000 Control Software sessssscssssessassaieetsvanrraaseacieyeennsaiernaaioreivianaton wanes 2 Safety WCU CRNA ccs aoe adie laced ed ecceta de lias ceiascas cea raek ca ease adc ated RET kiak 21 Satety precautions serens RER caressa ARSE IRSN 2 2 Labels on Typhoon FLA 9000 s ssssssssssssssssssssssssssssssssstssssssssttssstnssnttanstssstntanntnnnnnannosntsn neess Co Emergency procedure series cones tee eee 24 Recycling iM on AtON pisssesssssasSssssassssasncesncnsecencecasesseiyoenhestgestascasssssssotanadinsaatuestseatas beasasecseanaene 3 mstallation Reece one ne ie ere ere irae aaa a i a a iaa ery nr ner Bid SIE TEQUMEMESNS sicsscsssessssssccstgasscsssnsssccanssacetanascetayssengeuatazessnascetazadacn onsbscrdassteccazadcetebetencszsas atai a 3 2 Transporting the Typhoon FLA 9000 o nnnsecessssssssssssessssscecessnesteseseccesssssnnnnseseseeeeeessnnneteee 3 3 Unpacking the Typhoon FLA 9000 poss oc assessiessesiestenesenieeasies cise cesuienent 34 CONN CHONS sser E E A NE
18. UTION AN Activate the Typhoon FLA 9000 at least once every 30 days to execute calibra tion This calibration ensure that the SHG laser operates properly Failure to calibrate the Typhoon FLA 9000 every 30 days reduces the lifespan of the SHG laser Operation procedures 1 Turn on the Typhoon FLA 9000 and the computer 2 Wait until warm up and self diagnosis is completed and the scanner is ready Only the power lamp on the upper left panel is lit The automatic calibration has now been performed 3 Turn off the Typhoon FLA 9000 and the computer Replacing the filters in the Typhoon FLA 9000 See the instruction in Appendix C Replacing the filters on page 66 Getting Started with Typhoon FLA 9000 28 9607 66 AB 65 C Replacing the filters Appendix C Replacing the filters Remove the filter module from the scanner Step Action 1 Click the Filter Module button in the main window 2 Press the button on the filter change door 3 Grip the knob and open the filter change door 66 Getting Started with Typhoon FLA 9000 28 9607 66 AB C Replacing the filters Step Action 4 Pull out the filter module Remove and replace filters in the filter module Step Action 1 Pull the green locking lever to the left aano ALNO 28966408 Getting Started with Typhoon FLA 9000 28 9607 66 AB 67 68 C Replacing the filters Step PATER TRAY aano Tilt the filter backwards an
19. ater enters the Storage phosphor screen the sensitivity of the storage phosphor screen is reduced e Volatile solvents may deform the protective film on the storage phosphor screen 50 Getting Started with Typhoon FLA 9000 28 9607 66 AB 7 Exposing storage phosphor screens 7 1 Handling precautions Protect exposed storage phosphor screens from light Protect exposed storage phosphor screens from light until the reading is finished Store the storage phosphor screen correctly Remove the storage phosphor screen from the Typhoon FLA 9000 main unit when not in use Store the storage phosphor screens in a horizontal position protected from moisture 7 2 Preparing the storage phosphor screen Procure necessary tools and items Necessary items Storage phosphor screen cassette Radio isotope labeled samples Gloves Plastic film Soft lint free tissues Ethanol Clean the storage phosphor screen and the cassette Clean the surface of the storage phosphor screen and the inside of cassette with a soft tissue to remove dust and stains Erase the storage phosphor screen The FLA Image Eraser can erase the storage phosphor screen in around 15 minutes if it is not overly exposed For details refer to the FLA Image Eraser User Manual Dim the lighting e Dim the ambient light to 20 lux or less before opening or moving the storage phosphor screen without a cover Getting Started with Typ
20. bedded in the file where the image is saved and can be viewed with the analyzing software 4 Displays the laser and the filter used In phosphorimaging mode the laser is auto matically set to 635 nm and the filter is automatically set to IP Note To use the phosphorimaging mode the 635 nm laser must be loaded and the IP filter must be set If these conditions are not fulfilled the Reader Settings window for the phosphorimaging mode cannot be accessed 5 PMT set the voltage of the photo multiplier tube The higher the value the higher the sensitivity 6 Set the scanning area 1 Select a method in the drop down menu 2 Drag the red square to the desired position of the scanning area 3 Drag the sides of the red square as needed to adjust the size of the scanning area 7 Pixel Size set the pixel size Choose a small pixel size for high quality images Note that a small pixel size setting increases the reading time and the size of the image file 8 Save Condition click this button to save the current reading conditions in a file if desired For details refer to the User Manual 9 Load Condition click this button to load previously saved reading conditions if desired For details refer to the User Manual 8 2 Place the storage phosphor screen on the phosphor stage Step Action il Turn down the lighting in the room 2 Place the cassette with the exposed storage phosphor screen on
21. calchidcea tect crudioaeccd ss etlact ia deeeabiaathl EAEE RA Aei 64 PEE OUI PERCU INNO E AA E E iaaceasentitei at TE EE 65 Replacing the Miter Sisauna GGE 66 Default sample detection methods ss sssssessesssssssessresssssessssessseseesreseesessseseesseseessestsnesssoreesseseeseseesnesssesees 71 o e CH NE EE E E AE TAAI A EAEE ANENE A AIE AO EAA 74 Getting Started with Typhoon FLA 9000 28 9607 66 AB 1 Introduction 1 Introduction Purpose of the Operating Instructions The Operating Instructions provide you with the instructions needed to handle the Typhoon FLA 9000 system in a safe way Prerequisites In order to operate the Typhoon FLA 9000 in the way it is intended the following prerequisites must be fulfilled e You have read and understood the safety instructions outlined in this manual e The instrument is installed according to the instructions in this manual In this chapter This chapter contains important user information and a general description of the Typhoon FLA 9000 and its intended use 1 1 Important user information Read this before using the Typhoon FLA 9000 All users must read this entire manual to fully understand the safe use of Typhoon FLA 9000 Intended use Typhoon FLA 9000 is a fast laser scanner optimized for quantitative phosphorimaging ECL Plus Westerns visible single fluorescence and gel documentation Typhoon FLA 9000 is intended for research use only and shall not be used
22. cs a E aa ERETT 3 5 Software in tallAUON sensers enen 3 5 1 Install Typhoon FLA 9000 Control Software under Windows XP waeccccssssssssccssseeee 3 5 2 Install Typhoon FLA 9000 Control Software under Windows Vista 3 6 Preparations before starting Typhoon FLA 9000 sscvsssesessessosssesesessesesesesesssseseressee 4 OPeEroti M sess e aa E a a E e e ES 4T SDE POOR OV CNV sil ssena a N E EERE 4 2 Starting the instrument and the Typhoon FLA 9000 Control Software 5 Reading fluorescent SaMpleS saci cccs ssastSasdsicsesatectectasiesathateatstissesctesieeivemeelaeciciiaaia 5 1 Set the reading mode and reading Conditions oeeeeeeeecsssseeeeseseccessessssnsneseseeeeeeennnneeees 52 Place the sample on th Stige sasieenirisinnsinenunienenmanin ieis 5 3 Place the stage in Typhoon FLA 9000 assoc ccsescccoctassvasencensbondosssvsvervevendoncassdseoei eeccesestonvves 54 Regd thesompl 2 ee ee eee ee eee eee EE EER 6 Reading chemiluminescent samples scccssesececsscscsceccesesssscssscscececseesceeececsceees 6 1 Set the reading Mode and reading Conditions onneeessesssssesessscccsssssseessececeessnsneeees Ge Place the sample on the stage 6 nsccmnarinacenemanereentornaaannnen 6 3 Place the stage in the Typhoon FLA 9000 ccc isssssccsicscsesckcosssbasesensestsacsbensosssenesenaesssensdonds 64 Readthe samplessssinneenn a Ea EI TaN ToT 7 Exposing storage phosphor screens ccssssscscecsscsscessscsecssscsessceceessss
23. cscceessceceeees 7 1 Handling precautions cca ezacsoococtvossesicensosessanssnacsstbctesssssscsysoievcocrasnernsaacseeeeeeoosente 7 2 Preparing the storage phosphor Screen sssssesssssccsssssssssseecsscecssssnseesseeeeesessntseeeess 7 3 Exposing the storage phosphor Screen ou essssssssssssesescsscessneetsstsesceseessssnnesseseeeeeeessnttttees 8 Reading phosphorimaging samples s s ssesssssssessssesessssessessesessssssesseseosesresesssseseesese 8 1 S tthe redding CORIIN ONG xis sessecsterstdeserscesi directed eteihiessetedinus eto ae ence 8 2 Place the storage phosphor screen on the phosphor stage esssccccsssssseeeeeeseeeee Getting Started with Typhoon FLA 9000 28 9607 66 AB Table of Contents Table of Contents o a OO gt m 8 3 Place the phosphor stage in the Typhoon FLA 9000 ueesccsssssssscsssssessccsssssseescsssssseecsessssnuescessssnusscssssnneceesssseess 56 84 RECITES SOI DIG nsiisnnesguniasanianneun mu E R S iR 57 R gdding digitization Sample S sessies arrian E E E 59 9 1 Set the reading mode and reading Conditions ss cassiasccsddsssasdstesessearsisseicceeaeiantwasienceamacehaenenbsGieses 59 g2 Placethe sample on the Stage sereine a AE i ERAT RINE RNS 60 9 3 Place the stage in the Typhoon FLA 9000 s sssssssssssssssssssssssssssssssstsssstsssttnssstssnnsannnttsnntsnntnsanntannornanntsnnnssntnennnnrnnee 61 TI 1181 it VTS A 8 e a E a NCRTE eer TS eer Te ener ee 62 Daily MAIMTENANCE occas tesla sd eh
24. ctions 2 2 Labels on Typhoon FLA 9000 2 2 Labels on Typhoon FLA 9000 Typhoon FLA 9000 serial number The Typhoon FLA 9000 serial number is located on a label on the back of the instrument 96 GE Healthcare Bio Sciences AB 751 84 Uppsala Sweden FLUORESCENT IMAGE ANALYZER Model Typhoon FLA 9000 Serial No 123456 Frequency 50 60 Hz Supply Voltage 100 240 V c US Max Current 3 0A 1 5A Protection Class IP20 Manufacturing Year ACERS Mandanos FUJIFILM Corporation TOKYO 106 8620 JAPAN MADE IN JAPAN 405N101494 LABORATORY EQUIPMENT 19NS Symbols used in safety labels Label Meaning Warning Read the user documentation before using the system Do not open any covers or replace parts unless specifically stated in the user documentation The system complies with the requirements for electromagnetic compliance EMC in Australia and New Zealand C The system complies with applicable European directives 20 Getting Started with Typhoon FLA 9000 28 9607 66 AB 2 Safety instructions 2 2 Labels on Typhoon FLA 9000 Labels concerning use of hazardous substances Label Meaning This symbol indicates that the waste of electrical and electronic equipment must not be disposed as unsorted municipal waste and must be collected separately Please contact an authorized representative of the manufacturer for information concerning the decommissioning of equipment This symb
25. d GE monogram are trademarks of General Electric Company Typhoon is a trademark of GE Healthcare companies All third party trademarks are the property of their respective owners 2009 General Electric Company All rights reserved First published Oct 2009 All goods and services are sold subject to the terms and conditions of sale of the company within GE Healthcare which supplies them A copy of these terms and conditions is available on request Contact your local GE Healthcare representative for the most current information GE Healthcare Europe GmbH Munzinger Strasse 5 D 79111 Freiburg Germany GE Healthcare UK Limited Amersham Place Little Chalfont Buckinghamshire HP7 9NA UK GE Healthcare Bio Sciences Corp 800 Centennial Avenue P O Box 1327 Piscataway NJ 08855 1327 USA GE Healthcare Japan Corporation Sanken Bldg 3 25 1 Hyakunincho Shinjuku ku Tokyo 169 0073 Japan 28 9607 66 AB 11 2009
26. d pull it out from under the metal lip ant No 28066408 Place the new filter under the rear metal lip and lower the front edge of the filter Push the green locking lever to the right Make sure all filters are locked Getting Started with Typhoon FLA 9000 28 9607 66 AB C Replacing the filters Place the filter module in the scanner Step Action 1 Open the filter change door 2 Insert the filter module in the filter module holder 3 Close the filter change door Register the filter change in the Typhoon FLA 9000 Control Software Step Action 1 Click the Filter Module button in the main window U Fiteroduie Getting Started with Typhoon FLA 9000 28 9607 66 AB 69 C Replacing the filters Step Action 2 Click the desired filter position on the filter tray 3 Cancel OK 4 Click the Insert button to register the filter gt Insert TIP You can also drag the filter from Filter List to the desired filter position 5 Click the OK button 70 Getting Started with Typhoon FLA 9000 28 9607 66 AB D Default sample detection methods Appendix D Default sample detection methods 473 nm laser Reagent name Excitation wavelength Emission light wavelength Filter Alexa Fluor 488 495 519 LPB AttoPhos 482 560 LPB Cy 2 489 506 LPB DBR1 BPB1 DY 485XL 485 560 LPB ECL Plus 420 460 LPB EGFP 489 50
27. e the file after the reading 2 File Name enter the name of a file to save image data Getting Started with Typhoon FLA 9000 28 9607 66 AB 59 9 Reading digitization samples 9 1 Set the reading mode and reading conditions Part Description 3 Comment enter an optional comment The comment is embedded in the file where the image is saved and can be viewed with the analyzing software 4 Method Set the method to use in the scan 5 PMT set the voltage of the photo multiplier tube The higher the value the higher the sensitivity 6 Set the scanning area 1 Select a method in the drop down menu 2 Drag the red square to the desired position of the scanning area 3 Drag the sides of the red square as needed to adjust the size of the scanning area 7 Pixel Size set the pixel size Choose a small pixel size for high quality images Note that a small pixel size setting increases the reading time and the size of the image file 8 Save Condition click this button to save the current reading conditions in a file if desired For details refer to the User Manual 9 Load Condition click this button to load previously saved reading conditions if desired For details refer to the User Manual 10 Prescan Click to quickly prescan the sample at a resolution of 1000 um 9 2 Place the sample on the stage Placing a gel sample on the Fluor stage Note Step The sample must not be thick
28. equipment in a residential area is likely to cause harmful interference in which case the user will be required to correct the interference at his own expense This Class A digital apparatus complies with Canadian ICES 003 Cet appareil numerique de la class A est conforme a la norme NMB 003 du Canada This is a class A product In a domestic environment this product may cause radio interference in which case the user may be required to take adequate measures This instrument meets the laser radiation safety requirements specified in the Code of the Federal Regulations 21 CFR Chapter 1 Subchapter J This equipment is a class 1 Laser Product IEC60825 1 2001 EN60825 1 2002 Getting Started with Typhoon FLA 9000 28 9607 66 AB 1 Introduction 1 2 Regulatory information GE Healthcare Bio Sciences AB 751 84 Uppsala Sweden FLUORESCENT IMAGE ANALYZER MODEL Typhoon FLA 9000 SERIAL No MANUFACTURED FFTPT Complies with FDA performance standards for laser products except for deviations pursuant to Laser Notice No 50 dated June 24 2007 405N101495 This instrument conforms to the above mentioned Code of the Federal Regulations and is designed to be safe against laser radiation The laser leak level is suppressed to one that does not cause harm to the human body Class 1 for sufficiently safe operation provided that the user carries out operation including user maintenance of the instrument properly Reg
29. er than 30 mm For solutions the maximum height is 4mm Make sure there are no bubbles or gaps on the contact surface Action 60 Place the sample on the Fluor stage Getting Started with Typhoon FLA 9000 28 9607 66 AB 9 Reading digitization samples 9 2 Place the sample on the stage Step Action 2 Place the fluorescent plate for digitization on top of the sample 9 3 Place the stage in the Typhoon FLA 9000 Step Action 1 Open the lid of the Typhoon FLA 9000 2 Place the stage on the Typhoon FLA 9000 with the printed side of the frame facing up 3 Press the stage all the way in _ 4 Close the lid Getting Started with Typhoon FLA 9000 28 9607 66 AB 61 9 Reading digitization samples 9 4 Read the sample 9 4 Read the sample Click the Start Scan button to start reading Note If you click the Stop button during reading the part that has not been read yet will be saved as an image with a data value of 0 light intensity of 0 Note When you click the Stop button the reading is aborted You cannot start reading again from the location where reading stopped Adjust the display parameters Parameter Explanation Curve Adjust the gradations in the image Exponential Exponential gradation adjustment Linear Linear gradation adjustment Sigmoid Sigmoid gradation adjustment Magnification Zoom in or out Auto range scope Check this option to optimize the tones automatical
30. ere to save the file after the reading 2 File Name enter the name of a file to save image data Getting Started with Typhoon FLA 9000 28 9607 66 AB 45 6 Reading chemiluminescent samples 6 1 Set the reading mode and reading conditions Part Description 3 Comment enter an optional comment The comment is embedded in the file where the image is saved and can be viewed with the analyzing software Method set the method to use in the scan No laser or filter is used in the chemilu minescence mode PMT set the voltage of the photo multiplier tube The higher the value the higher the sensitivity Set the scanning area 1 Select a method in the drop down menu 2 Drag the red square to the desired position of the scanning area 3 Drag the sides of the red square as needed to adjust the size of the scanning area Pixel Size set the pixel size Choose a small pixel size for high quality images Note that a small pixel size setting increases the reading time and the size of the image file Save Condition click this button to save the current reading conditions in a file if desired For details refer to the User Manual Load Condition click this button to load previously saved reading conditions if desired For details refer to the User Manual 10 Prescan Click to quickly prescan the sample at a resolution of 1000 um 6 2 Place the sample on the stage Place
31. from the Start menu or use the desktop shortcut 36 Getting Started with Typhoon FLA 9000 28 9607 66 AB 4 Operation 4 2 Starting the instrument and the Typhoon FLA 9000 Control Software Step Action 5 The main window of the Typhoon FLA 9000 Control Software is displayed CONTROL SOFTWARE imagination at work O10 GANTRAL UICTING COMPANY ALL OMT RSID Technical Support hip daww gettes clences comvcontacy The condition is displayed in the Status area of the main window Status messages are as follows Message Explanation Disconnected Cannot recognize Typhoon FLA 9000 Please check connection and power Warm up Typhoon FLA 9000 is in self diagnosis Please wait Ready The unit is ready to use Getting Started with Typhoon FLA 9000 28 9607 66 AB 37 5 Reading fluorescent samples 5 Reading fluorescent samples 5 1 Set the reading mode and reading conditions Start the system 1 Turn on Typhoon FLA 9000 and the computer 2 Start the Typhoon FLA 9000 Control Software Set the reading conditions 38 Click the Fluorescence button in the main window to reach the Reader Settings window for the fluorescence mode Perform the tasks specified in the numbered table below All parts in the window are described in Section 1 4 Typhoon FLA 9000 Control Software on page 12 8 9 Pie tat Neb e ee i ais 2 ee prea o e a 7 Pee S ore ze Pixel Size i Je a Soe pea
32. he Multi stage Step Action 1 Place a gel sample with glass on the Multi stage 2 Carefully fold down the spring loaded glass holders on the glass 3 Tighten the screws on the glass holders as necessary Getting Started with Typhoon FLA 9000 28 9607 66 AB 41 5 Reading fluorescent samples 5 3 Place the stage in Typhoon FLA 9000 5 3 Place the stage in Typhoon FLA 9000 Placing the Fluor or Multi stage in the Typhoon FLA 9000 Step Action 1 2 4 Open the lid of the Typhoon FLA 9000 Place the stage on the Typhoon FLA 9000 with the printed side of the frame facing up ppor Close the lid 42 Getting Started with Typhoon FLA 9000 28 9607 66 AB 5 Reading fluorescent samples 5 3 Place the stage in Typhoon FLA 9000 Placing the LF glass plate stage in the Typhoon FLA 9000 Step Action 1 Open the lid of the Typhoon FLA 9000 2 Place the LF glass plate stage in the scanner 3 Push the LF glass plate stage in until it stops 4 Close the lid 5 4 Read the sample Click the Start Scan button to start reading Curve funeor 3 s P due Range Scope bo Bisi kal i fete foem Montesa a JA an Stop Manes Renting MAIS se rm armen Note If you click the Stop button during reading the part that has not been read yet will be saved as an image with a data value of 0 light intensity of 0 Note When you click the Stop button the reading is aborted You ca
33. he Typhoon FLA 9000 2 Turn off the computer and any peripheral devices 3 Disconnect the power cords and the USB connection 3 3 Unpacking the Typhoon FLA 9000 e Check the equipment for any apparent damage before starting installation e Document any damage carefully and contact your GE Healthcare representative Getting Started with Typhoon FLA 9000 28 9607 66 AB 25 3 Installation 3 4 Connections 3 4 Connections Communication Step Action 1 Connect a USB cable to the USB port on the rear side of the Tyohoon FLA 9000 2 Connect the other end of the USB cable to a USB port on the computer CAUTION e Do not connect any USB devices other than the Typhoon FLA 9000 to the computer in which the Typhoon FLA 9000 Control Software is installed e Use only the Typhoon FLA 9000 Control Software during reading Electrical power Connect the power cord of the Typhoon FLA 9000 to a grounded power outlet WARNING Use only power cords delivered or approved by GE Healthcare 3 5 Software installation Installation sequence Software installation is performed in the following sequence 1 Install the USB control driver 2 Install the USB function driver 3 Install the Typhoon FLA 9000 Control Software 26 Getting Started with Typhoon FLA 9000 28 9607 66 AB 3 Installation 3 5 Software installation 3 5 1 Install Typhoon FLA 9000 Control Software under Windows XP 3 5 1 Install Typhoon FLA 9000
34. hoon FLA 9000 28 9607 66 AB 51 7 Exposing storage phosphor screens 7 2 Preparing the storage phosphor screen e Protect the phosphor stage with the protective cover when removing an exposed storage phosphor screen from the cassette and placing it on the Phosphor stage Set the exposure time Set the exposure time so that the exposure finishes right before the reading starts Note TIP The exposure time of the storage phosphor screen is approximately one twentieth of the time required for X ray film Take this into consideration when setting the initial exposure time For increased image quality minimize the time between exposing the storage phosphor screen and reading it with the Typhoon FLA 9000 7 3 Exposing the storage phosphor screen Step Action 1 Zz Erase the storage phosphor screen completely Wrap the radio isotope sample with plastic film Make sure not to wrinkle the wrapping film Note When using a tritium storage phosphor screen to detect tritium place the sample directly on the storage phosphor screen A tritium storage phosphor screen can be used only once Open the top cover of the cassette Unlock the cassette by sliding the buttons on both sides up Getting Started with Typhoon FLA 9000 28 9607 66 AB 7 Exposing storage phosphor screens 7 3 Exposing the storage phosphor screen Step Action 4 Place the sample on the cassette Make sure that the sample surface faces up
35. illeur du Canada Freauer 50 60 Hz Supply Voltage 100 240 V 0A 1 5A Protection Clase IP20 usonarony govevenr Monufacturng Yaar TOYODA IPRS MADE IN JAPAN 405101494 USB Labels on left side of Typhoon FLA 9000 22 Getting Started with Typhoon FLA 9000 28 9607 66 AB 2 Safety instructions 2 2 Labels on Typhoon FLA 9000 Labels inside the Typhoon FLA 9000 Interlock parts Never insert any foreign object into the interlock parts to avoid potential laser exposure and injury 2 5 Emergency procedure This section describes how to do an emergency shutdown of the Typhoon FLA 9000 instrument The section also describes the results of a power failure Emergency shutdown Step Action 1 Click the Stop button in the Typhoon FLA 9000 Control Software 2 Turn off the Typhoon FLA 9000 by pressing the power switch on the right side of the in strument body 3 Disconnect the power cord from the power outlet Getting Started with Typhoon FLA 9000 28 9607 66 AB 23 2 Safety instructions 2 3 Emergency procedure Power failure The results of a power failure depends on the unit or units affected Unit affected by power failure Results Typhoon FLA 9000 instrument e The reading is interrupted immediately The instrument is in an undefined state e The data collected up to the time of the power failure is available in the file created when starting the scan Co
36. ions in a dialog with the text Windows needs your permission to continue Enter an administrator password if prompted then click Continue to proceed with the installation Step Action 1 Disconnect Typhoon FLA 9000 Control Software from the computer 2 Open the control panel and click Classic View in the upper left corner 3 Open Add Hardware Getting Started with Typhoon FLA 9000 28 9607 66 AB Step 3 Installation 3 5 Software installation 3 5 2 Install Typhoon FLA 9000 Control Software under Windows Vista Action 10 In the Add Hardware dialog click the Next button Select Install the hardware that manually select from a list Advanced and click the Next button Select Show All Devices and click the Next button Da eel From the bst belom select the type ol haedman pou are ost alee I you do ret nee the hindame category you mart chh Show All Devices Comman hadas types Ccghay stagiar IDE ATAMTAA conteclines SPIELE 1204 4 competi porter ARICLE 1304 4 eve IELE 124 Dux host cortotens a faet E lt Back Net Coes Click the Have Disk button Insert the Typhoon FLA 9000 Control Software CD and click the Browse button Select to install the driver from the Typhoon FLA 9000 Control Software CD Lock re GA DVO AW Dae Tretenn FLA 9000 io 02 onm fa emote Lacan ue jsm vee D ota m m SORENTO E a Compar as Open the USB Control folder lakn G OVD RW Dae Tohon
37. isplay parameters Parameter Explanation Curve Adjust the gradations in the image Exponential Exponential gradation adjustment Linear Linear gradation adjustment Sigmoid Sigmoid gradation adjustment Magnification Zoom in or out Auto range scope Check this option to optimize the tones automatically Getting Started with Typhoon FLA 9000 28 9607 66 AB 57 8 Reading phosphorimaging samples 8 4 Read the sample 58 Parameter Explanation Color Select the colors used to display the data Value Negative Gray White Positive Gray Red Green Blue Color32 Color of low values Black Black Black Black Blue Color of high values Black White Red Green Blue Red Getting Started with Typhoon FLA 9000 28 9607 66 AB 9 Reading digitization samples 9 Reading digitization samples 9 1 Set the reading mode and reading conditions Start the system 1 Turn on Typhoon FLA 9000 and the computer 2 Start the Typhoon FLA 9000 Control Software Set the reading conditions Click the Digitization button of the main window to reach the Reader Settings window for the digitization mode e Perform the tasks specified in the numbered table below Note All parts in the window are described in Section 1 4 Typhoon FLA 9000 Control Software on page 12 O Oo FPF WNB Fite Size about 1439 MB x2 Part Description 1 Image folder specify where to sav
38. ly 62 Getting Started with Typhoon FLA 9000 28 9607 66 AB 9 Reading digitization samples 9 4 Read the sample Parameter Explanation Color Select the colors used to display the data Value Color of low Color of high values values Negative Gray White Black Positive Gray Black White Red Black Red Green Black Green Blue Black Blue Color32 Blue Red Getting Started with Typhoon FLA 9000 28 9607 66 AB 63 A Daily maintenance Appendix A Daily maintenance Cleaning the stage 64 CAUTION Wear gloves to prevent direct contact with chemical substances Step Action 1 Remove the stage from the main body of the instrument 2 Wipe the stage with a sponge moistened with a fluorescence free neutral detergent 3 Thoroughly rinse the stage with water and dry with a lint free cloth 4 Place the stage in the main body of the instrument Getting Started with Typhoon FLA 9000 28 9607 66 AB B Periodic maintenance Appendix B Periodic maintenance Cleaning the outside of Typhoon FLA 9000 Clean the outside with a moist soft cloth and a mild detergent Wipe afterwards with a dry soft cloth Maintenance of the SHG laser If you use the SHG laser in the Typhoon FLA 9000 it will require periodical calibration When the Typhoon FLA 9000 is switched on it automatically executes calibration You do not need to do a manual calibration if the Tyohoon FLA 9000 is used at least once a month CA
39. mputer running the Typhoon FLA e The computer shuts down immediately 9000 Control Software e The run continues but no data is saved 2 4 Recycling information General instructions for disposal When taking the Typhoon FLA 9000 out of service the different materials must be separated and recycled according to national and local environmental regulations Specific instructions for disposal Measure the superficial radio isotope pollution of the instrument body and storage phosphor screen as mentioned in the radiation hazard prevention section of the User Manual If the pollution level exceeds the limit dispose of the instrument body as radioactive waste Otherwise dispose of the materials according to applicable laws and regulations for disposal of industrial waste 24 Getting Started with Typhoon FLA 9000 28 9607 66 AB 3 Installation 3 Installation 3 1 Site requirements Note The Typhoon FLA 9000 is intended for indoor use only Power supply 100 to 240 VAC 3 0 to 1 5 A Line frequency 50 to 60 Hz Placement Stable horizontal surface out of direct sunlight Ambient temperature 10 to 30 C Humidity 30 to 70 non condensing Maximum altitude 2000 m above sea level 3 2 Transporting the Tyohoon FLA 9000 The Typhoon FLA 9000 weighs approximately 97 kg and requires at least two persons to lift and move Precautions before moving the instrument Step Action 1 Turn off t
40. n FLA 9000 Control Software under Windows Vista Step Action 7 If User Account Control UAC is enabled in Windows Vista a dialog displays the message An unidentified program wants access to your computer Click Allow 8 Click the Finish button The installation of Typhoon FLA 9000 Control Software is now completed 3 6 Preparations before starting Tyohoon FLA 9000 Checklist before starting Typhoon FLA 9000 e Typhoon FLA 9000 is placed on a sturdy horizontal surface e Typhoon FLA 9000 is connected to a grounded wall outlet e The air intake fan on the rear side of Typhoon FLA 9000 is unobstructed e The air exhaust fan on the left side of Typhoon FLA 9000 is unobstructed e There are no objects on top of Typhoon FLA 9000 Note Before turning on the instrument open the lid and make sure that the stage rack is empty Then close the lid securely 34 Getting Started with Typhoon FLA 9000 28 9607 66 AB 4 Operation 4 Operation 4 1 Operation overview Using Typhoon FLA 9000 comprises a series of steps outlined below Detailed explanations are provided in subsequent chapters Turn on the Typhoon FLA 9000 Phosphorimaging samples Start the computer and launch the Typhoon FLA 9000 Control Software Expose the storage phosphor screen Set the reading parameters Place the stage and the sample in the Typhoon FLA 9000 Read the sample 4 2 Starting the instrument and the Typhoon FLA 9000 Control Softwa
41. nnot start reading again from the location where reading stopped Getting Started with Typhoon FLA 9000 28 9607 66 AB 43 5 Reading fluorescent samples 5 4 Read the sample Adjust the display parameters Parameter Explanation Curve Adjust the gradations in the image Exponential Exponential gradation adjustment Linear Linear gradation adjustment Sigmoid Sigmoid gradation adjustment Magnification Zoom in or out Auto range scope Check this option to optimize the tones automatically Color Select the colors used to display the data Value Color of low Color of high values values Negative Gray White Black Positive Gray Black White Red Black Red Green Black Green Blue Black Blue Color32 Blue Red 44 Getting Started with Typhoon FLA 9000 28 9607 66 AB 6 Reading chemiluminescent samples 6 Reading chemiluminescent samples 6 1 Set the reading mode and reading conditions Start the system 1 Turn on Typhoon FLA 9000 and the computer 2 Start the Typhoon FLA 9000 Control Software Set the reading conditions Click the Chemiluminescence button to reach the Reader settings window for the chemiluminescence mode cnt Perform the tasks specified in the numbered table below Note All parts in the window are described in Section 1 4 Typhoon FLA 9000 Control Software on page 12 Aron Fluorescence Stage inn Dn aA FPF WUN e Part Description 1 Image folder specify wh
42. ol indicates that the product contains hazardous materials in excess of the limits established by the Chinese standard SJ T11363 2006 Requirements for Concen tration Limits for Certain Hazardous Substances in Electronics Labels concerning laser light Label Meaning CAUTION Avoid exposure to the laser beam when the lid is open CAUTION Class 3B Laser product when open and interlock defeated Avoid exposure to the beam Do not attempt to defeat the safety interlocks under the sample lid or behind the filter door or otherwise try to gain access to the interior of the instrument through any other opening Exposure to laser light can cause injury Viewing the laser light directly can cause blindness Getting Started with Typhoon FLA 9000 28 9607 66 AB 21 2 Safety instructions 2 2 Labels on Typhoon FLA 9000 Labels on rear and right side of Typhoon FLA 9000 This device complies with part 15 of the FCC Rules Operation is subject to the following two conditions 1 this device may not cause harmful interference and 2 this device must accept any interference received including interference that may cause undesired operation FLUORESCENT IMAGE ANALYZER 3000 Serano This Class A digital apparatus meets all requirements of the Canadian Interference Causing Equipment Regulations Cet appareil numerique de la classe A respecte toutes les exigences du Reglement sur le materiel bou
43. ose No not this time and click the Next button Welcome to the Found New Hardware Wizard Insert the installation CD select Install the software automatically Recommended and click the Next button Click the Finish button to complete the installation Getting Started with Typhoon FLA 9000 28 9607 66 AB 29 3 Installation 3 5 Software installation 3 5 1 Install Typhoon FLA 9000 Control Software under Windows XP Install Typhoon FLA 9000 Control Software Windows XP Step Action 1 Insert the Typhoon FLA 9000 Control Software CD 2 Locate and double click the file Typhoon FLA 9000 msi 3 In the Typhoon FLA 9000 InstallShield Wizard click the Next button 4 Read the license text If the license agreement is not acceptable please contact a GE Healthcare representative see back cover of this manual for contact information Select accept the terms in the license agreement and click the Next button 5 Select destination folder in the dialog Seam e Click the Next button to install the software at the default folder C Program Files e Click the Change button to install to a different folder 6 Click the Install button 7 Click the Finish button so complete the installation of Typhoon FLA 9000 Control Software 3 5 2 Install Typhoon FLA 9000 Control Software under Windows Vista Install the USB Control Driver Windows Vista 30 Note During software installations you may be asked to confirm your act
44. re Note Before turning on the instrument open the lid and make sure that the stage rack is empty Then close the lid securely Getting Started with Typhoon FLA 9000 28 9607 66 AB 35 4 Operation 4 2 Starting the instrument and the Typhoon FLA 9000 Control Software Step Action 1 Push the power switch on the right side of the instrument to the I position 2 When the instrument is turned on the On Off conditions of the indicator lamps change as shown below ee ee Immediately after turning on power D POWER BUSY ERROR 4 takes up to 30 seconds a A During self check or startup adjustment 4 takes up to 10 minutes Ready for operation POWER BUSY ERROR Starting the Typhoon FLA 9000 Control Software CAUTION Do not insert a Storage phosphor screen in Typhoon FLA 9000 before turning on the machine If an imaging plate is detected during the self diagnosis of the Typhoon FLA 9000 the sensitivity of the Storage phosphor screen may deteriorate The scanned data can then not be guaranteed Step Action 1 Turn on Typhoon FLA 9000 2 Turn on the computer 3 Make sure that Typhoon FLA 9000 has completed the warm up after which only the power lamp on the upper left panel on the front of the Typhoon FLA 9000 is lit 4 Start Typhoon FLA 9000 Control Software
45. re dialog Getting Started with Typhoon FLA 9000 28 9607 66 AB 3 Installation 3 5 Software installation 3 5 2 Install Typhoon FLA 9000 Control Software under Windows Vista Step Action 4 Select Install this driver software anyway Gana es v See deta 5 A successful installation message appears Click the Close button Install Typhoon FLA 9000 Control Software Windows Vista Note During software installations you may be asked to confirm your actions in a dialog with the text Windows needs your permission to continue Enter an administrator password if orompted then click Continue to proceed with the installation Step Action il Insert the Typhoon FLA 9000 Control Software CD 2 Locate and double click the file Typhoon FLA 9000 msi 3 In the Typhoon FLA 9000 InstallShield Wizard dialog click the Next button 4 Read the license text If the license agreement is not acceptable please contact a GE Healthcare representative See the back cover of this manual for contact information Select accept the terms in the license agreement and click the Next button 5 Select destination folder in the dialog e Click the Next button to install the software at the default folder C Program Files e Click the Change button to install to a different folder 6 Click the Install button Getting Started with Typhoon FLA 9000 28 9607 66 AB 33 3 Installation 3 5 Software installation 3 5 2 Install Typhoo
46. small pixel size setting increases the reading time and the size of the image file 8 Save Condition click this button to save the current reading conditions in a file if desired For details refer to the User Manual 9 Load Condition click this button to load previously saved reading conditions if desired For details refer to the User Manual 10 File Size the estimated size of the result data file is presented 11 Reading Time the estimated time required for the scan is presented 12 Top return to the main window 13 Start Scan start the scan The sample must be loaded before starting a scan 14 Prescan perform a quick scan at a resolution of 1000 um Table 1 1 Parameter Operating system Windows XP Professional SP2 or later Minimum requirement or Windows Vista Business SP 1 or later Getting Started with Typhoon FLA 9000 28 9607 66 AB Parameter Internal memory Minimum requirement 1GB Processor Intel Core 2 Duo processor Hard drive 80 GB Monitor resolution 1280 x 1024 pixels Other requirements One USB 2 0 port DVD ROM drive Getting Started with Typhoon FLA 9000 28 9607 66 AB 1 Introduction 1 4 Typhoon FLA 9000 Control Software 15 2 Safety instructions 2 Safety instructions 2 1 Safety precautions Introduction The Typhoon FLA 9000 is powered by mains voltage and is used to image samples that may be hazardous Before
47. that are fulfilled by the Tyohoon FLA 9000 system 6 Getting Started with Typhoon FLA 9000 28 9607 66 AB 1 Introduction 1 2 Regulatory information Manufacturing information Requirements Content Name and address of manufacturer GE Healthcare Bio Sciences AB Bj rkgatan 30 SE 751 84 Uppsala Sweden Name and ID of notified body INTERTEK SEMKO AB NB 0413 Place and date of declaration Uppsala Sweden Sept 2009 Identity of person authorized to sign Declaration See EC Declaration of Conformity of Conformity International standards This product fulfills the requirements of the following standards Standard Description Notes EN 61010 1 IEC 61010 1 UL Safety requirements for electrical 61010 1 IEC 61010 2 81 equipment for measurement control CAN CSA C22 2 No 61010 1 and laboratory use EN 61326 1 EMC emissions and immunity require Harmonized with VCCI Class A ments for electrical equipment for 2004 108 EC FCC Part 15 B Class A ai control and laboratory ICES 003 Class A EN ISO 12100 1 12100 2 Safety of machinery Basic concepts Harmonized with general principles for design 2006 42 EC EN ISO 14121 1 14121 2 Safety of machinery Principles of risk Harmonized with assessment 2006 42 EC EN 60825 1 IEC 60825 1 Safety of laser products Harmonized with 2006 95 EC USA 21 CFR Chapter I Subchapter Safety of laser products J Part 1040 10 Laser Products
48. the sample on the Fluor stage with the chemiluminescent side face down AletcloleteiaM ulitstxtcetws 46 APOR STADE 4040 Getting Started with Typhoon FLA 9000 28 9607 66 AB 6 Reading chemiluminescent samples 6 2 Place the sample on the stage Note Tape the corners of membrane samples to the stage This reduces the effect of physical vibrations 6 3 Place the stage in the Typhoon FLA 9000 Step Action al Open the lid of the Typhoon FLA 9000 2 Place the stage on the Typhoon FLA 9000 with the printed side of the frame facing up m 4 Close the lid Getting Started with Typhoon FLA 9000 28 9607 66 AB 47 6 Reading chemiluminescent samples 6 4 Read the sample 6 4 Read the sample Click the Start Scan button to start reading Note If you click the Stop button during reading the part that has not been read yet will be saved as an image with a data value of 0 light intensity of 0 Note When you click the Stop button the reading is aborted You cannot start reading again from the location where reading stopped Adjust the display parameters Parameter Explanation Curve Adjust the gradations in the image Exponential Exponential gradation adjustment Linear Linear gradation adjustment Sigmoid Sigmoid gradation adjustment Magnification Zoom in or out Auto range scope Check this option to optimize the tones automatically 48 Getting Started with Typhoon FLA 9000 28 9607
49. to the User Manual 9 Load Condition click this button to load previously saved reading conditions if desired For details refer to the User Manual 10 Prescan Click to quickly prescan the sample at a resolution of 1000 um 5 2 Place the sample on the stage CAUTION Always wear gloves protective glasses and a lab coat or similar when handling samples Getting Started with Typhoon FLA 9000 28 9607 66 AB 39 5 Reading fluorescent samples 5 2 Place the sample on the stage Placing a gel sample on the Fluor stage Note e The sample must not be thicker than 30 mm For solutions the maximum height is 4mm e Make sure there are no bubbles or gaps on the contact surface Step Action 1 Place the sample on the Fluor stage 2 Place the fluorescent plate for digitization on top of the sample Placing a titer plate on the Multi stage Step Action 1 Place the titer plate frame on the Multi stage d i 2 Place the titer plate in the desired position in the titer plate frame 40 Getting Started with Typhoon FLA 9000 28 9607 66 AB Placing a DIGE gel on the LF glass plate stage Step Action 5 Reading fluorescent samples 5 2 Place the sample on the stage 1 Place one or two DIGE gels on the LF glass plate stage 2 Fasten each glass with the front and rear glass holders 3 Press down each glass holder and tighten the screw Placing a gel sample with glass on t
50. ulatory compliance of connected equipment Any equipment connected to the Typhoon FLA 9000 should meet the safety requirements of EN 61010 1 IEC 61010 1 or relevant harmonized standards Within EU connected equipment must be CE marked 1 3 The Typhoon FLA 9000 laser scanner Major features e Imaging of gel membrane etc dyed or labelled with various fluorescent dyes at high sensitivity and high resolution e Yielding very high resolution images equivalent to X ray film e Features very high sensitivity wide dynamic range reliable linearity and high resolution e Reusable storage phosphor screens e High resolution high sharpness and linearity greatly exceeding film systems e System configuration requiring no darkroom or automatic developer Getting Started with Typhoon FLA 9000 28 9607 66 AB 9 1 Introduction 1 3 The Typhoon FLA 9000 laser scanner Parts and functions Part Function 1 Indicator lamps 2 Lid 3 Handle 10 Getting Started with Typhoon FLA 9000 28 9607 66 AB 1 Introduction 1 3 The Typhoon FLA 9000 laser scanner Part Function 4 Instrument cover 5 Power cord connector 6 Power switch 7 Filter holder 8 Filter door 9 USB connector Accessories included Fluor stage L Multi stage es Phosphor stage LF glass plate stage Titer plate plugin HA Getting Started with Typhoon FLA 9000 28 9607 66 AB 11
51. z Te ali 2 pm Cpm 400 pm A200 pm eer Do vaso ms C19 pm pg Area Fluorescence Stage swe sone 5 wares Bice Bo i8 r Pou r na 7E vason koa 6 2nd File Size about 5 01 MB x2 T 10 Reading Time about 13m 0s f l E 12 E Top File Format Gel E Prescan O Start Scan 11 d 14 13 Part Description 1 Image folder specify where to save the file after the reading 2 File Name enter the name of a file to save image data 3 Comment enter an optional comment The comment is embedded in the file where the image is saved and can be viewed with the analyzing software Getting Started with Typhoon FLA 9000 28 9607 66 AB 13 1 Introduction 1 4 Typhoon FLA 9000 Control Software Part Description System requirements 14 4 Method set the method to use in the scan Up to 4 scans can be performed in a row all with individual methods 5 PMT set the voltage of the photo multiplier tube The higher the value the higher the sensitivity Click to increase the number of scans Click to decrease the number of scans 6 Set the scanning area 1 Select a method in the drop down menu 2 Drag the red square to the desired position of the scanning area 3 Drag the sides of the red square as needed to adjust the size of the scanning area 7 Pixel Size set the pixel size Choose a small pixel size for high quality images Note that a
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