PIPETMAN® P
Contents
1. n Ux The data given in the table conform to the ISO 8655 2 Standard 23 EN PIPETMAN P EN 15 SPARE PARTS A pU P2 and P10 only u 24 P2 F144801 and P10 F144802 Description P2 P10 A Push button F144781 F144782 C Connecting nut F123654 123654 H Tip holder F144816 144819 1492 Seal O ring F144861 F144862 X Adaptor set of 5 F144879 144879 E X F144876 144876 Coloris clips mixed colors set of 10 F161301 F131301 P20 F123600 and P100 F123615 Description P20 P100 A Push button F144783 F144784 C Connecting nut F123654 F123654 E Tip ejector F123657 144605 H Tip holder F123353 F144602 51 52 Seal O ring set of 5 F144863 F144864 Coloris clips mixed colors set of 10 F161301 F131301 P200 F123601 and P1000 F123602 Description P200 P1000 A Push button F144785 F144786 C Connecting nut F123654 123654 E Tip ejector F123658 123659 H Tip holder F123305 123371 1492 Seal O ring set of 5 F144865 144866 Coloris clips mixed colors set of 10 F161301 131301 P5000 F123603 and P10ml F161201 Description P5000 P10ml Push button F144787 F161281 H Tip holder F123608 161263 1482 Seal O ring set of 5 F144867 161829 Coloris clips mixed colors set of 10 F161301 131301 Filters set of 10 F161280 161280 25 EB PIPETMAN P EN EC DECLARATION O2. C or with a soft cloth and brushes Small round brushes with soft plastic bristles may be used to clean the interior of the tip holder 4 Rinse the individual components with distilled water 5 Leave the parts to dry by evaporation or wipe them with a clean soft cloth or lint free tissue 6 Reassemble the pipette as described in Maintenance 20 Decontamination Autoclaving The upper part body and the piston assembly of the pipette are not autoclavable Only the follow ing parts may be autoclaved tip ejector tip holder and connecting nut The O ring and seal are not autoclavable they may be cleanedor replaced with the one specified in Spare Parts 1 Clean the parts to be autoclaved especially the tip holder 2 Putthe parts in an autoclaving sack 3 Autoclave for 20 minutes at 121 C 0 1 MPa 4 Check that the parts are dry before re assembling the pipette 5 Setthe pipette aside to stabilize at room tem perature Chemical Decontamination You may choose to decontaminate your pipette chemically in accordance with your own proce dures Whatever decontaminant you use check with the supplier of the decontaminant that it is compatible with the plastics used in the construc tion of the pipette and does not attack any of the following plastics PA Polyamide PC Polycar bonate POM Polyoxy methylene or PVDF Polyvinylidene Fluoride Upper Part body 1 Wipe the upper part body of
3. contact your local Gilson authorized Service Center Tip ejector and Tip holder These parts must be changed if they are ac cidentally damaged or attacked chemically You should also remove these parts for cleaning or decontamination purposes A cloth dampened with ethanol may be used to routinely clean the outside of the pipette Changing the Tip ejector 1 To remove the tip ejector keep the tip ejector button depressed and pull down on the flanged upper part of the tip ejector with the other hand moderate force is required 16 A Color coded push button C Connecting nut E Tip ejector H Tip holder P Piston assembly 1 Seal 52 O ring EN PIPETMAN P EN 2 Torefitthe tip ejector keep the tip ejector button depressed slide the end of the tip ejector over the end of the tip holder and push the plastic end of the tip ejector back into the body of the pipette until itis gripped firmly by the tip ejector rod metal Changing the Tip holder 1 Remove the tip ejector see above 2 Unscrew the connecting nut by turning it coun terclockwise by hand 3 Carefully separate the lower and upper parts 4 Remove the piston assembly O ring and seal 5 Clean autoclave or replace the tip holder 6 Reassemble the pipette refer to the figure 7 Tighten the connecting nut turn by hand clockwise 8 Refit the tip ejector see above Servicing the Piston Assembly You may remove t
4. eoejday Jepjou diy 79110018 03 ayjadid uunjau nu Buroeuuoo Ajjealueyoaw 10 jeorueuo si uoisid 9500 SI Japjou dij Are the O ring and seal undamaged and free of chemical damage Ifansweris NO clean orreplacethe damaged part If the answer is YES refer to the table below you may need to clean or replace the piston assembly O ring and seal Pipetman must be adjusted reca gt f you see a bubble in the aspirated liquid librated after the piston assembly is changed and free of chemical damage sued 00 908099 eas 0 Wom ayeuldse j uoM ayadig sued y oq eoejdau eas 10 Buu Q Woy ejduues si ayjadig uonoy asne a qissod wojdwis EB PIPETMAN P EN 14 Before returning any pipette ensure that itis completely free of chemical biologi cal or radioactive contamination Refer to chapter Cleaning and decontamination 11 LEAK TEST This test may be performed at any time to check that the pipette does notleak especially after performing a maintenance or decontamination procedure If a pipette fails this test you should replace the O ring and seal and repeat this test after making sure that the pipette is correctly reassembled P2 to P200 fita Gilson Diamond Tip set the pipette to the maximum volume given the specifications and pre rinse aspirate the set volume from a beaker of
5. the pipette with a soft cloth or lint free tissue impregnated with the chosen decontaminant 2 Wipe the upper part of the pipette with a soft cloth orlint free tissue impregnated with distilled water or sterile water Lower Part The following components only can be im mersed in a decontaminant solution tip ejector tip holder connecting nut piston assembly 21 EN PIPETMAN P EN 1 Disassemble the pipette as described in Maintenance 2 Immersethe components inthe de contaminant solution or wipe them according the instructions given by the manufacturer or supplier of the decontaminant 3 Rinse the individual components with distilled or sterilized water 4 Leave the parts to dry by evaporation or wipe them with a clean lint free tissue or a soft cloth 5 Reassemble the pipette as described in Maintenance 14 SPECIFICATIONS Pipetman P is a high quality pipette that offers excellent accuracy and precision The figures given in the Gilson Maximum Permissible Errors table were obtained using Gilson Diamond Tips These figures are only guaranteed by using genuine Gilson Diamond Tips Refer to Chapter 7 Gilson Diamond Tips Each pipette is inspected and validated by qualified technicians accordingto the Gilson Quality System Gilson declares that its manufactured pipettes comply with the requirements of the ISO 8655 Standard by type testing The adjustmentis carried out under strictly defined and moni
6. EN PIPETMAN P EN Other languages can be consulted and downloaded on our website www gilson com EN PIPETMAN P EN CONTENTS page 1 INTRODUCTION 2 2 PARTS CHECK LIST 3 3 DESCRIPTION 3 4 OPERATING RANGES 4 5 SETTING THE VOLUME 5 6 PIPETTING 6 7 GILSON DIAMOND TIPS 9 8 PERSONALIZING YOUR PIPETTE 11 9 GLP FEATURES 12 10 TROUBLESHOOTING 13 11 LEAK TEST 15 12 MAINTENANCE 16 13 CLEANING AND DECONTAMINATION 19 14 SPECIFICATIONS 22 15 SPARE PARTS 24 EC DECLARATION OF CONFORMITY 25 1 INTRODUCTION Congratulations on acquiring your new Pipetman P Enjoy the following features Continuously adjustable designed to dispense precise volumes of liquid safely Large range of volumes eight models cover a range from 0 1 to 10 mL for many applications P2 and P10 measurement and transfer of micro volumes DNA sequencing and enzyme assays P20 P100 P200 P1000 measurement and transfer of general aqueous solutions acids and bases P5000 P10ml measurement and transfer of large volumes Volumeis continuously adjustable within the use ful volume range using the digital volumeter Tip ejector and disposable tips mean no need to handle tips protecting the user from contamination Carry over between samples eliminated by using a new tip from a Tipack box for each transfer Nominal volume i e pipette model is shown on the color
7. F CONFORMITY The company GILSON S A S 19 avenue des Entrepreneurs BP 145 95400 Villiers le Bel France Tel 33 0 1 34 29 50 00 Fax 33 0 1 34 29 50 20 www gilson com Hereby certifies on its sole responsibility that the products listed below Pipetman P2 P10 P20 P100 P200 P1000 P5000 and P10ml comply with the requirements of the following European 98 791 EC i Directive on In Vitro Medical Diagnostic Annex Ill self declared Villiers le Bel October 5th 2004 S Solotareff R Pascal General Manager Quality Director 26 NOTES 27 LT801117 X 2005 Gilson SAS All right reserved May 2005 English Printed in France World Headquarters Gilson Inc 3000 W Beltline Hwy P Box 620027 Middleton WI 53562 0027 USA Telephone 1 800 445 7661 or 1 608 836 1551 Fax 1 608 831 4451 Gilson SAS 19 avenue des Entrepreneurs B P 145 95400 Villiers le Bel France Telephone 33 1 34 29 50 00 Fax 33 1 34 29 50 20
8. coded push button Personalize your Pipetman by fitting a Coloris identification clip to the connecting nut 5 colors 2 PARTS CHECK LIST Just take a moment to verify that the following items are present Pipetman P User s Guide Sample pack of tips Safety bag Adhesive id tags strip of 4 Certificate of conformity including bar code sticker 3 DESCRIPTION UG Please refer to the schematic on the next page to see the items A Colorcoded push button for setting the volume aspirating and dispensing B Body or handle EN PIPETMAN P EN C Connecting nut to upper part body or handle of removable tip holder D Diamond tip E Tip ejector F Tip ejector button H Tip holder removable for cleaning and de contaminating T Thumbwheel for setting the H volume V Volumeter D 341 4 OPERATING RANGES Model Range 2 0 2 2yL P10 1 10 pL 20 2 20 2n Fr P100 20 100 uL P200 50 200 uL 1000 200 1000 uL P5000 1 5mL e P10ml 1 10 mL Ux With a precise pipetting technique see Gen eral Guidelines for good pipetting P2 may be used to aspirate volumes down to 0 1 uL P10 at 0 5 uL and P200 at 30 uL 5 SETTING THE VOLUME The volume of liquid to be aspirated is set using the volumeter The volumeter consists of three number dials which are read from top most sig nificant digit to bottom least signi
9. distilled water maintain the pipette in the vertical position and wait for 20 seconds if a water droplet appears at the end of the tip there is a leak if you see no droplet re immerse the tip below the surface of water the water level inside the tip should remain constant if the level goes down there is a leak P1000 to P10ml fit a Gilson Diamond tip set the pipette to the maximum volume given in the specifications aspirate the set volume from a beaker of distilled water maintain the pipette in the vertical position and wait for 20 seconds 15 EB PIPETMAN P EN if a water droplet appears at the end of the tip there is a leak 12 MAINTENANCE Routine maintenance will help keep your pipette in good condition ensuring a continued high level of performance Maintenance is limited to cleaning or autoclaving the parts specified under Cleaning and Decontamination or to replacing the push button connecting nut tip ejector tip holder seal and O ring Pipetman P2 and P10 should notbe disassembled so you may only replace the push button tip ejector dual position tip ejector and its adapter with these pipettes if the tip holder is damaged the piston may also be damaged After replacing any parts you should verify A the performance of your pipette following the verification procedure available on the Gilson website www gilson com If the pipette needs to be readjusted please
10. e Diamond Tips Volume Range P2 D10 DL10 DF10 DFL10 0 1 uL to 2 uL P10 D10 DL10 DF10 DFL10 0 5 uL to 10 pL P20 DF 30 D200 2 uL to 20 uL DF30 20 uL to 30 uL P100 D200 DF100 20 uL to 100 uL DF100 30 uL to 100 uL P200 D200 DF200 D300 DF300 30 uL to 200 uL P1000 D1000 DF1000 D1200 200 pL to 1 mL P5000 D5000 1mLto5mL P10 ml D10 ml 1mLto 10 mL 8 PERSONALIZING YOUR PIPETTE Personal Label Application Name Tag In addition to the four round adhesive Stickers supplied with your pipette you can identify your pipette with a Coloris clip Made of poly acetal these clips accept permanent ink and wipe clean with ethanol Ideal for recording service or calibration dates Perfect for quick visual identification Also useful for identifying pipettes dedicated to specific ap plications or as a warning when there is risk of contamination 11 EN PIPETMAN P EN These brightly colored clips snap quickly over the connecting nut of Pipetman models P2 to P1000 Becausethey don trequire dismantling or changing original parts there s no danger of the pipette s calibration being affected Tip ejector Dual position Adaptor for P2 and P10 The component parts of a tip ejector assembly are a metallic rod and a blue plastic connector which goes into the body of the pipette For P2 and P10 a dual position adaptor plastic is also required the metallic rod is shaped so that the adaptor may be clipped to i
11. ed and decontaminated However because Pipetman P2 and P10 contain miniaturized parts it is best not to disassemble these pipettes your self please contact your local Gilson authorized Service Center I amp You may refer to the Decontamination proce dure available on the Gilson website www gilson com Liquid must never enter the upper part body of any pipette 19 EB PIPETMAN P EN Cleaning The pipette must be cleaned as described below before it is decontaminated Soap solution is recommended for cleaning Pipetman P External 1 Remove the tip ejector 2 Wipethe tip ejector with a soft cloth or lint free tissue impregnated with soap solution 3 Wipe the entire pipette with a soft cloth or lint free tissue impregnated with soap solution to remove all dirty marks Ifthe pipette is very dirty a brush with soft plastic bristles may be used 4 Wipe the entire pipette and the tip ejector with a soft cloth or lint free tissue impregnated with distilled water 5 Refit the tip ejector and allow the pipette to dry Internal The following components only can be im mersed in a cleaning solution connecting nut tip ejector tip holder piston assembly seal and O ring 1 Disassemble the pipette as described in Maintenance 2 Set aside the upper part in a dry and secure location 3 Clean the individual components of the lower part of the pipette using an ultrasonic bath 20 minutes at 50
12. ficant digit A markeris used to setexactor intermediate volumes using the scale on the bottom dial The dials are colored either black or red to indicate the position of the decimal point according to the model see examples The volume is set by turning Push button the thumbwheel or the push button The push button makes it easier and quicker to set volumes especially when wearing gloves The thumb wheel may be turned to slowly reach the required setting To obtain maximum accura cy when setting the volume proceed as follows when decreasing the volume setting slowly reach the required setting making sure not to overshoot the mark when increasing the volume setting pass the required value by 1 3 of a turn and then slowly decrease the volume to reach the volume mak ing sure not to overshoot the mark Model Color of volumeter numbers Black Red P2toP200 P1000 P5000 0 1 mL and 0 01 mL mL P10ml mL 0 1 mL EB PIPETMAN P EN Example for each pipette P10 P20 P100 P2 1 2 1 125uL 75 125 75uL 200 1000 P5000 10 aJ 125uL 0 75mL 125mL 7 5 mL Toavoid parallax errors make sure thatthe volume indicator and the selected volume marking are in your direct line of vision At close range you may find it helps to close one eye zs 1 0 0 0 LO me O w O0 6 PIPETTING 1 Fit a new Gilson Diamond Tip Plastic tips are fo
13. he piston assembly for cleaning purposes only If the piston assembly is changed the pipette must be adjusted and calibrated in a Gilson authorized Service Center The piston assembly must not be auto claved 1 Remove the tip ejector see above 2 Unscrew the connecting nut by turning it coun terclockwise by hand 5 Carefully separate the lower and upper parts A A Remove the piston assembly O ring and seal Clean and decontaminate the piston assembly Reassemble the pipette refer to the figure Tighten the connecting nut turn by hand clockwise 8 Refit the tip ejector see above Changing the O ring The O ring and seal are normally to be found on the piston they must not be autoclaved if worn or damagedin any way chemical or mechanical they must be replaced The dimensions of the O ring vary according to the model of pipette 1 Remove the tip ejector see above 2 Unscrew the connecting nut by turning it coun terclockwise by hand Carefully separate the lower and upper parts Remove the piston assembly O ring and seal Clean or replace the seal then the O ring Reassemble the pipette refer to the figure page 17 7 Tighten the connecting nut turn by hand clockwise 8 Refit the tip ejector see above 13 CLEANING AND DECONTAMINATION Pipetman P is designed so that the parts normally in contact with liquid contaminants can easily be clean
14. imum volume Does the volumeter operate smoothly without hitching If the answer to any of these questions is NO it is probable the pipette has been damaged as the result of a mechanical shock or chemical dam age in which case it should be returned to your supplier for repair If the answer to these questions is YES continue as follows Is the tip ejector straight Is the tip ejector free of chemical damage Remove tip ejector is the tip holder undamaged 13 J0199 9 di1 ay eae dag Jepjou din aui aoejdau 01 puowelg u0sjI5 esf J0199 9 di Jepjou dn pebeweg 6011 Ayenb moq 11 U0p 10 jJO SC Jepjou din ayy eoejday 7910018 03 ayjadid uunjau nu Buroeuuoo usui sued yjog 928 Buium 10181900 nu Bunoeuuoo usui Jepjou di peDeue Ajeorueuoau s uojsid 8s00 si nu Bunoauuo eas 0 Wom anbiuyoa 10je18do 19911020 8s00 SI J9pjOU di a s d 10 S Y dId nu Bunoeuuoo nu Bunoeuuoo 8s00 si Dunoeuuo epjou di pamaaasu f dispense the liquid into its original vessel then ensure that the tip is properly immersed below the surface ofthe liquid and aspirate more slowly if a bubble appears again replace the tip 995 azenou si nu Buoeuuoo usui 795001 S nu Bunoeuuo Jepjou dn au
15. r a single application they must not be cleaned for reuse Push the tip holder into the tip using a slight twisting motion to ensure a firm and airtight seal 05 1 For P2 and P10 tip ejector extensions are supplied 2 P5000 and P10ml insert a filter into the tip holder before fitting a tip If the filter gets dirty it should be replaced with a clean one 3 P5000 and P10ml these models are not equipped with tip ejectors 2 Pre rinse the tip Some liquids e g protein containing solutions and organic solvents can leave a film of liquid on the inside the wall of the tip pre rinse the tip to minimize any errors that may be related to this phenomenon Pre rinsing consists of aspirating the first vol ume of liquid and then dispensing it back into the same vessel or to waste Subsequent volumes that you pipette will have levels of accuracy and precision within specifications 3 Aspirate Press the push button to the first stop this corresponds to the set volume of liquid Hold the pipette vertically and immerse the tip in the liquid see immersion depth table Release the push button slowly and smoothly to top position to aspirate the set volume of liquid Wait one second time depends on model see table then withdraw the pipette tip from the liquid You may wipe any droplets away from the out side of the tip using a medical wipe however if you do so take care to avoid touching the tip s orifice 4 Di
16. smoothly never turn the pipette upside down never lay the pipette on its side when there is liquid in the tip 7 If you use the same tip with a higher volume pre rinse the tip 8 For volatile solvents you should saturate the air cushion of your pipette by aspirating and dispensing the solvent repeatedly before aspirating the sample 9 When pipetting liquids with temperatures dif ferent to the ambient temperature pre rinse the tip several times before use 10 You may remove the tip ejector see Mainte nance to aspirate from very narrow tubes 11 After pipetting acids or other corrosive liquids that emit vapors removethetip holderand rinse the piston and O ring and seal with distilled water 12 Do not pipette liquids having temperatures above 70 C or below 4 C The pipette can be used between 4 C A and 40 C butthe specifications may vary according to the temperature see Chapter 14 for controlled conditions of use 7 GILSON DIAMOND TIPS Gilson Diamond Tips are made to the highest specifications strict quality control is maintained throughout the manufacturing process Diamond Tips are used to calibrate Pipetman P EB PIPETMAN P EN therefore for optimum performance youare strongly advised to use Gilson s Diamond Tips with your Pipetman P Diamond Tips have the Gilson logo engraved on their collar ensuring that you have a genuine Gilson product Toensure accuracy and precision Gil
17. son s Quality Assurance System focuses onthe following critical parameters DiamondTips are made from pure polypro pylene virgin metal free to avoid the possibility of contamination They are available sterilized Optimized shape revised collar for optimum sealing thin walls and fine point making them easier to mount more flexible with no vortexing and improved precision Diamond Tips are free from even microscopic defects especially atthe orifice All surfaces are smooth and hydrophobic thereby avoid ing the excessive retention of liquids that causes poor accuracy and a lack of precision e Mold and cavity references Gilson ID Number are marked on the collar Loge ensuring the traceability for quality assurance purposes batch numbers appear on all packages bags and boxes They form an air tight seal with the tip holder preventing the leaks that cause poor accuracy and a lack of precision e They may be autoclaved at 121 C for 20 minutes at 0 1 MPa Diamond Filter Tips Filtertips are used when sample to sample sample to pipette or sample to operator contamination must be avoided 10 Because of there unique construction Diamond Filter DF Tips may be autoclaved without risk of damage to the filters Gilson s sterilized Diamond Filter Tips are certified free of detect able RNase DNase DNA RNA and protease Table Tips to Use for Best Results Pipett
18. spense Place the end of the tip against the inside wall of the recipient vessel at an angle of 10 to 40 Press the push button slowly and smoothly to the first stop Wait for at least a second then press the push button to the second stop to expel any residual liquid from the tip Keep the push button pressed fully down and while removing the pipette draw the tip along the inside surface of the vessel Release the push button smoothly EN PIPETMAN P 5 Ejectthetip by pressing firmly on thetip ejector button Top 151 Stop 2nd Stop General Guidelines for Good Pipetting 1 Make sure that you operate the push button slowly and smoothly 2 When aspirating keep the tip at a constant depth below the surface of the liquid refer to the table Table Immersion Depth amp Wait Time Model Immersion Wait Time Depth mm Seconds P2 1 1 P10 1 1 P20 2 3 1 P100 2 4 1 P200 2 4 1 P1000 2 4 2 3 P5000 3 6 4 5 P10ml 5 7 4 5 3 Change the tip before aspirating a different liquid sample or reagent 4 Change the tip if a droplet remains at the end of the tip from the previous pipetting operation 5 Each new tip should be pre rinsed with the liquid to be pipetted 6 Liquid should never enter the tip holder to prevent this press and release the push button slowly and
19. t in either of two positions Pipetman P2 and P10 are deliv ered with the adaptor in place and configured to accept DL10 tips When you use D10 tips which are shorter you must reposition the adaptor as follows 1 Pull the adaptor away from the metallic rod 2 Turn the adaptor through 180 3 Refit the adaptor so that the end of the metallic rod engages the shorter slot of the adaptor 4 Finally check that end of the metallic rod is correctly seated in the appropriate slot of the adaptor the dimple on the metallic rod must be engaged in the corresponding hole on the adaptor 9 GLP FEATURES These are as follows Serial Number engraved on body of the pipette 12 Bar Code Serial Number on the box and with the certificate can be transferred IDTag Application or User 10 TROUBLESHOOTING You may be able to identify and to correct the problem by reference to the following table If you can t solve the problem contact your Gilson representative However a quick inspection of the pipette may help you isolate the problem E3 You may download from the Gilson website www gilson com the 2 minutes inspection which shows how to perform a quick diagnosis of your pipette Is the operating rod straight Is the operating rod free of chemical damage Are the volumeter digits clear and easy to read Can you set any volume in the useful range Can you set the nominal max
20. tored conditions ISO 8655 6 Basis of adjustment Ex Reference temperature 20 C Relative humidity 50 Barometric pressure 101 kPa Use of distilled water grade 3 ISO 3696 Ten measurements for each test volume which are Nominal Volume 50 of Nominal Volume and the minimum or 10 of Nominal Volume 22 Maximum Permissible Errors Model Volume uL Gilson IS0 8655 Reference Accuracy Precision Accuracy Precision Systematic Random Systematic Random error pL error uL error pL error uL P2 Min 02 0 024 lt 0 012 0 08 0 04 F144801 05 0 025 lt 0 012 008 lt 0 04 Max 2 0 030 x0 0014 008 0 04 P10 Min 0 025 lt 0 012 012 lt 0 08 F144802 5 0 075 lt 0 030 012 0 08 Max 10 0100 0 040 012 0 08 Min 2 010 lt 003 020 0 10 P20 5 010 lt 004 020 lt 0 10 F123600 10 010 lt 005 020 lt 0 10 Max 20 020 x006 020 lt 0 10 P100 Min 20 035 lt 010 080 lt 0 30 F123615 50 040 lt 0 12 080 lt 0 30 Max 100 080 lt 015 080 lt 0 30 P200 Min 50 0 50 lt 020 160 lt 0 60 F123601 100 080 lt 025 160 lt 0 60 Max 200 160 lt 030 160 lt 0 60 P1000 Min 200 3 x 0 6 8 x30 F123602 500 4 1 0 8 x30 Max 1000 8 1 5 8 x30 P5000 1000 12 x3 x40 lt 15 F123603 2000 12 x5 40 lt 15 Max 5000 30 lt 8 40 lt 15 Min 1 mL 30 x6 60 30 P10ml 2mL 30 x6 60 30 F161201 5 mL 40 lt 10 60 lt 30 Max 10 mL 60 lt 16 60 x30
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