View Your SDS-PAGE Results Instantly, No More Gel Stain and De
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1. sample treatment buffer oxidation of reducing reagent such as DTT could reduce sharpness and brightness of some protein bands The recommended storage time of instant Band loading buffer at room temperature is one week If more than one week adding freshly made DTT to Instant Band will restore the sharpness and brightness The final concentration of DTT in Instant Band should not be over 20 mM DTT Other reducing reagents such as TCEP 2 mM will also work well 3 Do post translational modifications affect Instant Bands No 4 Some protein bands get less sharp over time why Oxidation of protein reduces band sharpness Add freshly made DTT to Instant Bands DTT concentration should not be over 20 mM 5 Can I do Western Blot experiments using Instant Bands stained samples Yes Instant Bands do not affect Western Blot results Alternatively you can use Instant bands stained sample as a control lane to check expression level of the target protein and verify the transfer efficiency 6 Why do I see precipitation in Instant Bands that has been stored at 4 degree The precipitation is SDS It will disappear when temperature is raised to at room temperature Related Products e Fluorescent Protein M W Marker e EZ Viewer LED Transilluminator e EZ PAGE the next generation of precast gel EZBiolab Visit us at www ezbiolab com Setting High Standards for Scientific Excellence Contact us at info ezbiolab com2. EZBiolab z lavstant View Your SDS PAGE Results Instantly Bands No More Gel Stain and De Stain User s Manual Instant Band sample treatment buffer sample loading buffer pre stains a protein sample for SDS PAGE Protein samples are stained fluorescently during sample treatment prior to electrophoresis You can observe results directly after electrophoresis on an UV or LED blue transilluminator This product is e Simple Completely eliminate the stain destain procedure without introducing any extra steps e Fast Protein bands in the gel can be visualized and pictured right after electrophoresis e Sensitive The sensitivity of detection of proteins is higher than the conventional Coomassie blue e Convenient The Gel doesn t need to be removed from the gel cassettes when using a LED transilluminator e Flexible The gel pre stained with Instant Band can still be processed by Coomassie Blue staining e Clean No contamination of organic solvents to our environment and no mess of Coomassie Blue to your lab Experiment Scheme of Instant Band Instant Bands Instantly Product Product Catalog Package Amount Instant Band Protein Sample Treatment Buffer PFSOO1 2 x Instant Bands 1 ml tube gt 800 lanes for SDS PAGE 5 ul loading volume each lane Instant Bands User s Manual Directions 1 Mix Instant Band Sample Treatment Buffer with a protein sample in 1 1 ratio 2 Heat the sample at 90 100 C for 10 minutes M
3. PES Buffer pH 6 5 5 4 2 HEPES Buffer pH 7 5 5 5 4 10 mM 20 mM 30 mM 50 mM DTT 5 5 5 4 1 3 5 m DMSO F 5 5 5 2M 4M 6M 8M Urea 5 5 4 3 5 10 20 Glycerol 5 4 3 For protein under extreme conditions such as high concentration of urea or low pH lt 5 please use Enhanced Instant Band EZBiolab Visit us at www ezbiolab com Setting High Standards for Scientific Excellence Contact us at info ezbiolab com Instant Bands User s Manual Application The product is best used for SDS PAGE electrophoresis to check protein expression determine the purity of a protein sample track target protein during purification and western blot as well as to exam immune precipitation results The product is not recommended for experiments to cut protein bands from a gel for sequencing mass spectrometry analysis or antibody production Frequent Asked Questions and Trouble Shooting 1 Why can t ready to use protein M W markers I purchased from other vendors be stained by Instant Band effectively Instant Band is specially formulated to enhance its staining efficiency Most of the ready use protein molecular weight markers are not compatible with Instant Bands EZBiolab provides pre stained fluorescent protein M W marker Alternatively markers can be generated by staining untreated proteins with Instant Bands 2 How about the stability of Instant Band Instant Band dye itself is stable However same as the regular
4. ake sure your heating block is gt 90 C to let the sample be sufficiently heated 3 The sample is ready for gel electrophoresis 4 After electrophoresis place the gel on an UV or LED blue transilluminator to view protein bands and take pictures Because light emitted by LED is transmittable through glass or plastics the gel does not need to be removed from the gel cassette Instant Bands 0 minute Coomassie Blue 4 hours Coomassie Blue 1 2 3 5 68 F7 B 1 23 4 5 68 7 B 3 Hours 1 3 5 and 7 were treated by Instant Band loading buffer E a 2 4 6 and 8 were treated by the regular loading buffer 0 Minutes Storage and Stability e Immediately store the package at 20 C upon receiving e Instant Band Sample treatment Buffer is stable for 12 month at 20 C 8 weeks at 4 degree and 1 week at room temperature e Like regular sample treatment buffer oxidation of reducing reagent such as DTT is the major problem for long term storage of Instant Band at room temperature Some protein bands could become less sharp and less bright if Instant Band is stored at RT for more than a week Add freshly made DTT will restore band sharpness and brightness The final concentration of DTT in Instant Band should not be over 20 mM e Always avoid light EZBiolab Visit us at www ezbiolab com Setting High Standards for Scientific Excellence Contact us at info ezbiolab com Instant Bands User s Manual Additional Features 1 Ge
5. ls stained by Instant Band can still be stained by Coomassie blue if it is wanted Perform Coomassie blue stain experiments according to the standard protocol 2 Instant Band is compatible to Western Blot Western Blot experiments can be performed using the standard protocol Proteins transferred onto the blot membrane can be directly visualized before and after antibody binding which helps to identify the target proteins Alternatively Instant Bands stained samples can be run as a control lane to verify transfer efficiency of the target proteins Conditions and Factors M W Marker _ Instant Band is specially formulated to enhance its stain efficiency Most of ready use protein molecular weight markers are not compatible with Instant Band EZBiolab provides pre stained fluorescent protein M W markers Alternatively markers can be generated by staining untreated proteins with Instant Bands Effect of pH Low pH reduces stain efficiency If pH of a sample solution is below 5 we suggest you to adjust pH to above 7 for the best results High pH gt 7 does not affect Instant Band Other Factors Proteins are effectively stained by Instant Bands under normal conditions Table below summarizes the effect of common reagents found in protein solution Control is 5 0 1 0 5 1 Triton 100 5 5 5 vo a Chaps 5 5 5 Glycoside 5 5 5 Tween 5 5 5 25 mM 50 mM 100 mM 7 Pi Buffer pH7 4 5 5 5 Tris Buffer pH 7 4 5 5 3 Fa PI
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