UNICORN® OS version 1.10 - GE Healthcare Life Sciences
Contents
1. run will stop Data will be saved up to that point depending on how AutoSave has been set Action To avoid power failure connect a UPS Uninterruptible Power Supply to both system and computer Computer requirements Computer Pentium 75 MHz minimum with at least 1 free full length ISA expansion slot For the maximum configuration of four systems connected a Pentium 133 M Hz or higher processor is required with four full length ISA expan sion slots About 16 M b addi tional RAM memory for each extra system is also required to run the systems smoothly H owever Amersham Biosciences does not recommend more then one instrument per computer The computer holds the method and if it crashes all runs con nected to the system will stop 32 Mb RAM minimum 24 M b 1 Gb minimum 240 M b 800x600 pixels with minimum 256 colors O S 2 Warp version 3 Any printer supported by OS 2 HP Desk et 660C 690C or 870Cxi and HP Laser et 5M P or 6M P recommended For network installations of UNICORN OS RAM memory H ard disk Graphics O perating system Printer N etwork N ovell N etWare version 4 02 or later Connections Etherlink III network card with 3C5x9 SY S driver version dated M arch 10 1995 or later Amersham e Biosciences2. AMERSHAM B UNICORN OS version 1 10 Important advice on installation The following actions must be taken when installing an instrument or changing Strategy 1 Calibrate the P 50 pump 2 Calibrate the pressure of pump P 6000 Important advice on operation UNICORN OS V1 10 Running a method Loading and Support Weight must be entered in the Variables Other parameters can be the same as default Check that the correct column volume is given When using the FIX FLOT or ADJFLOT templates for flow through coupling only DNA couplings are programmed for flow through RNA or 2 0M eare programmed for recycling System HOLD in method The Strategy instructions VOL_Amid VOL_Cap VOL_Thio and VOL_Oxid set the system on HOLD when turning valves and calculating and activating flow rates If you press PAUSE followed by CONT INUE or just press CONTINUE during system HOLD the flow of reagent will be interrupted and the wrong amount will be added to the column Action Do not press CONTINUE or PAUSE or give any manual commands during a System hold M ethod Editor N ote that a Cross reference list is saved with a speci fic sequence so that all saved sequences have their own unique Cross reference list If an open sequence is deleted and replaced with a new one this new sequence will inherit the Cross reference list Refer to the User M anual for more information Untitled window On some Compaq computers O S 2 will n
3. S some windows are already closed down and it might be impossible to open the M ain menu module to quit UNICORN OS Action N ever select Shutdown in O S 2 when UN I CORN OS is running Too many Watch instructions If a Watch instruction calls a block named e g Peak which contains a new Watch instruction that also calls the block Peak the message Too many Watches appears in the text instruction win dow and a very large number of blocks will be shown Action You can still save and run the method DEFAULT report format If a DEFAULT report format has been defined and File Report is selected the chromatogram layout set tings will be the same as on the screen and not as specified in the DEFAULT report format Action If you want to use the DEFAULT report for mat chromatogram layout you have to manually select the DEFAULT format from the list of available formats O pening or printing results To open or print a large result file can take several minutes depending on the computer Action Wait at least 10 minutes before rebooting the computer if you suspect a program crash Amersham i e Biosciences M ethod and Run To open or download a method to the instrument can take several minutes depending on the computer Action Wait at least 10 minutes before rebooting the computer if you suspect a program crash Lost text on print outs All text tick marks peak labels etc may be missing on your landscape format
4. chromatogram print outs Action If your printer supports Postscript try and change the printer driver to a Postscript driver Template Long runs A combination of very large template methods with up to 250 blocks and a long sequence gt 30 bases may mean that Printout Preview Start of Run and View of log book can take more than 5 minutes OligoPilot Il Communication If the communication between the computer and cer tain valves is not established the SystemC ontrol RunData will display the valve position as 0 Action Reboot both ControlBox and Computer If communication is broken during a run the valve position is not updated and will display the last valve position You will not be able to see the communica tion problem in the LogBook This problem has occurred only a few times The new version has been improved to prevent it H owever if the problem still occurs follow the actions described below Action Reboot both ControlBox and Computer and re run the synthesis Screen and Power saver functions in computers and O S 2 should be turned off to not interfere with the UNICORN OS software Action Disable screensaver and Autodim in O S 2 and disable Powersave in BIOS or Computer manu facturer software Calibration Calibrate the P 50 pump every 2 weeks or before very important runs Usually the calibration value is very stable Power failure If the whole system or just the computer has a power failure the
5. ot start up properly An Untitled window appears and the com puter locks Action Install the latest available display driver for your computer e g Compaq CL5434 Display Driver Support for OS 2 2 1X and 3 0 version 1 23 or Compaq Q Vision 1024 1280 Display Driver Support for OS 2 2 1X and 3 0 version 2 30 Importing edited Evaluation procedures If you try to replace an existing Evaluation procedure in a method with another that has the same name but in which the Report layout has been edited the Evaluation procedure is not always replaced correctly 56 3055 33 Edition AA O S C ENCES Information letter Action Remove the Evaluation procedure from the list in Run setup Then save the method Finally import the edited Evaluation procedure and save the method again Peak Integration W hen the peak area values that are calculated during manual not on line Peak Integration are important perform the integration by using Time base to get the best precision Preview in File Report W hen Preview has been selected in File Report and you click outside the Preview window the window becomes hidden but still open behind the UN l CORN OS desktop This can cause the computer to lock up at later stages Action Do not click outside the Preview window when open Shutdown If UNICORN OS is minimized and you select Shutdown in O S 2 a message asking to Quit UN l CORN OS appears When you then enter UN l CORN O
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